Efficient biological synergist for biochemical treatment of integrated circuit wastewater and application of efficient biological synergist

Abstract

The invention discloses a high-efficiency biological synergist for biochemical treatment of integrated circuit wastewater and application of the high-efficiency biological synergist, and relates to the technical field of wastewater treatment. The specific preparation method comprises the following steps: adding the microbial flora, the enzyme preparation, the trace elements, the algae substances, the organic acid, the regulator and the nutrient substances into the solvent, and mixing to obtain the biological synergist. Wherein the microbial flora can oxidize ammonia nitrogen into nitrate and reduce the nitrate into nitrogen, so that the ammonia nitrogen is removed, and the total nitrogen content is reduced; the enzyme preparation can accelerate the biochemical reaction process; the trace elements provide necessary nutrition support for the growth of microorganisms; the algae substances are beneficial to adjusting the ecological balance of the system; the organic acid can regulate the pH value of the wastewater and buffer the toxic action of metal ions, salts and toxic compounds; the regulator is used for promoting the growth and metabolism of microbial flora; and the nutrient substances promote the growth and reproduction of microorganisms. Through the combined action of the substances, the removal efficiency of the biological synergist on ammonia nitrogen and total nitrogen is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of wastewater treatment, and in particular to a high-efficiency biosynergist for biochemical treatment of integrated circuit wastewater and application thereof. Background Art

[0002] With the rapid development of the integrated circuit industry, the treatment of wastewater generated during its production process has become increasingly challenging. IC wastewater has a complex composition, containing large amounts of metal ions, fluorides, and organic pollutants. These substances have a strong inhibitory effect on microorganisms in the biochemical system, making it difficult for traditional wastewater treatment processes to effectively remove pollutants such as ammonia nitrogen and total nitrogen from the wastewater, making it difficult for the effluent to meet discharge standards. Wastewater treatment systems often suffer from poor nitrification, with chronically elevated effluent ammonia nitrogen, a near-absence of denitrification, and little variation between influent and effluent total nitrogen, resulting in poor denitrification efficiency. This makes it difficult to meet total discharge requirements. Some electronic wastewater contains numerous impurities and poor biodegradability. Toxic substances such as fluorides significantly impact the biochemical system, damaging nitrification and hindering microbial growth. To address these issues, the addition of bio-enhancers is currently commonly used to enhance the functionality of wastewater treatment systems. However, existing bio-enhancers, when dealing with the complex composition of IC wastewater, suffer from limited microbial activity enhancement and insufficient resistance to toxic substances, making them inadequate for the efficient and stable operation of IC wastewater biochemical treatment systems. Therefore, there is still a need for an efficient bio-enhancer to effectively remove pollutants such as ammonia nitrogen and total nitrogen in wastewater. Summary of the Invention

[0003] The purpose of the present invention is to provide a high-efficiency biosynergist for the biochemical treatment of integrated circuit wastewater and its application, so as to solve the problem that existing sewage treatment processes are difficult to effectively remove pollutants such as ammonia nitrogen and total nitrogen in wastewater.

[0004] In order to solve the above technical problems, the present invention specifically provides the following technical solutions: The invention discloses a biosynergist, comprising a microbial flora, a functional additive and a solvent; the microbial flora comprises nitrosomonas, nitrobacter and denitrifying bacteria, and the mass proportion of the microbial flora in the biosynergist is 3%-6%.

[0005] Preferably, the functional aids include enzyme preparations, trace elements, algae substances, organic acids, regulators and nutrients.

[0006] More preferably, the enzyme preparation includes at least one of urease, nitrate reductase and nitrite reductase, and the mass proportion of the enzyme preparation in the biosynergist is 1%-2%.

[0007] More preferably, the trace elements include EDTA-Fe, EDTA-Mn, EDTA-Zn and EDTA-Mo, and the mass proportion of the trace elements in the biosynergist is 0.05%-0.15%.

[0008] More preferably, the algae substance is spirulina powder, and the mass proportion of the algae substance in the biosynergist is 0.5%-1%.

[0009] More preferably, the organic acid includes citric acid, malic acid and lactic acid, and the mass proportion of the organic acid in the biosynergist is 0.05%-0.1%.

[0010] More preferably, the regulator comprises a naphthylacetic acid derivative and 2-ethyl-3-methyl-quinoline-4-carboxylic acid. The naphthylacetic acid derivative is prepared from naphthylacetic acid and 4-hydroxy-3-methoxyphenylethanol, and the weight proportion of the regulator in the biosynergist is 0.01%-0.1%. The synergistic use of the naphthylacetic acid derivative and 2-ethyl-3-methyl-quinoline-4-carboxylic acid improves the denitrification efficiency of the microbial flora by regulating the metabolic pathways of Nitrosomonas, Nitrobacter, and denitrifying bacteria in the microbial flora, thereby reducing the ammonia nitrogen and total nitrogen content in the wastewater.

[0011] More preferably, the nutrients include glucose, yeast extract and peptone, and the mass proportion of the nutrients in the bio-enhancer is 1%-2%.

[0012] Preferably, the solvent is water, and the mass proportion of the solvent in the biosynergist is 88.65%-94.39%.

[0013] The invention also discloses the use of any of the above-mentioned biosynergists in the preparation of purified water.

[0014] The present invention discloses a method for preparing a biosynergist, comprising: Microbial flora, enzyme preparations, trace elements, algae substances, organic acids, regulators and nutrients are added into a solvent and mixed to obtain a biological enhancer.

[0015] Preferably, the microbial flora includes Nitrosomonas, Nitrobacter and Denitrifying Bacteria.

[0016] More preferably, the mass ratio of Nitrosomonas to Nitrobacter is 1:1-2.

[0017] More preferably, the mass ratio of the usage amounts of Nitrosomonas to denitrifying bacteria is 1:1-3.

[0018] Preferably, the mass proportion of the microbial flora in the biosynergist is 3%-6%.

[0019] Preferably, the enzyme preparation comprises at least one of urease, nitrate reductase and nitrite reductase.

[0020] Preferably, the enzyme preparation accounts for 1%-2% by mass in the biosynergist.

[0021] Preferably, the trace elements include EDTA-Fe, EDTA-Mn, EDTA-Zn and EDTA-Mo.

[0022] More preferably, the mass ratio of EDTA-Fe to EDTA-Mn is 1:0.5-1.

[0023] More preferably, the mass ratio of EDTA-Fe to EDTA-Zn is 1:0.5-1.

[0024] More preferably, the mass ratio of EDTA-Fe to EDTA-Mo is 1:0.05-0.25.

[0025] Preferably, the mass proportion of trace elements in the biosynergist is 0.05%-0.15%.

[0026] Preferably, the algae material is spirulina powder.

[0027] Preferably, the mass proportion of algae in the biosynergist is 0.5%-1%.

[0028] Preferably, the organic acid includes citric acid, malic acid and lactic acid.

[0029] More preferably, the mass ratio of citric acid to malic acid is 1:0.5-1.

[0030] More preferably, the mass ratio of citric acid to lactic acid is 1:0.5-1.

[0031] Preferably, the mass proportion of the organic acid in the biosynergist is 0.05%-0.1%.

[0032] Preferably, the modulator includes naphthyl acetic acid derivatives and 2-ethyl-3-methyl-quinoline-4-carboxylic acid.

[0033] More preferably, the mass ratio of the naphthylacetic acid derivative to the 2-ethyl-3-methyl-quinoline-4-carboxylic acid is 1:0.5-1.2.

[0034] Preferably, the mass proportion of the regulator in the biosynergist is 0.01%-0.1%.

[0035] Preferably, the nutrients include glucose, yeast extract and peptone.

[0036] More preferably, the mass ratio of glucose to yeast extract is 1:0.5-1.

[0037] More preferably, the mass ratio of glucose to peptone is 1:0.25-0.5.

[0038] Preferably, the mass proportion of the nutrients in the bio-synergist is 1%-2%.

[0039] Preferably, the solvent is water, and the mass proportion of the solvent in the biosynergist is 88.65%-94.39%.

[0040] The present invention discloses a method for preparing a naphthylacetic acid derivative, comprising: Add naphthaleneacetic acid to dichloromethane, followed by N,N'-dicyclohexylcarbodiimide, 4-dimethylaminopyridine, and 4-hydroxy-3-methoxyphenylethanol. Allow to react at 20-30°C for 8-12 hours. After the reaction, filter to obtain a filtrate, concentrate the filtrate, add water, and then extract with ethyl acetate. Separate the organic layer. Wash and dry the organic layer to obtain a naphthaleneacetic acid derivative.

[0041] Preferably, the usage ratio of naphthylacetic acid to dichloromethane is 1 g:5-15 ml.

[0042] Preferably, the mass ratio of naphthylacetic acid to N,N'-dicyclohexylcarbodiimide is 1:1-1.6.

[0043] Preferably, the mass ratio of naphthylacetic acid to 4-dimethylaminopyridine is 1:0.05-0.1.

[0044] Preferably, the mass ratio of naphthylacetic acid to 4-hydroxy-3-methoxyphenylethanol is 1:0.6-1.3.

[0045] Preferably, the usage ratio of naphthylacetic acid to water is 1 g:8-15 ml.

[0046] Preferably, the washing is carried out sequentially with acetic acid solution, sodium hydroxide solution and water.

[0047] More preferably, the acetic acid solution consists of acetic acid and water, and the mass ratio of acetic acid to water is 1:16-23.

[0048] More preferably, the sodium hydroxide solution consists of sodium hydroxide and water, and the mass ratio of sodium hydroxide to water is 1:20-28.

[0049] More preferably, in the process of preparing the biosynergist of the present invention, in addition to using naphthylacetic acid derivatives and 2-ethyl-3-methyl-quinoline-4-carboxylic acid, N-acetyl-L-tyrosine ethyl ester can also be used. The synergistic use of N-acetyl-L-tyrosine ethyl ester can further induce the division and growth of Nitrosomonas, Nitrobacter and Denitrifying bacteria cells in the microbial flora, enhance the absorption and metabolic regulation of nutrients, and thus improve the degradation ability of ammonia nitrogen.

[0050] Preferably, the mass ratio of the naphthylacetic acid derivative to N-acetyl-L-tyrosine ethyl ester is 1:0.8-1.5.

[0051] Compared with the prior art, the present invention has the following beneficial effects: This invention proposes a highly efficient biosynergist for the biochemical treatment of integrated circuit wastewater and its application. The biosynergist is prepared by adding microbial flora, enzyme preparations, trace elements, algae, organic acids, regulators, and nutrients to a solvent and mixing them together. Nitrosomonas and Nitrobacter in the microbial flora oxidize ammonia nitrogen into nitrate, while denitrifying bacteria reduce nitrate to nitrogen gas under anoxic conditions, removing ammonia nitrogen and reducing total nitrogen content. The enzyme preparation accelerates biochemical reactions; trace elements provide essential nutritional support for microbial growth; algae help maintain the system's ecological balance; organic acids adjust the wastewater's pH and buffer the harmful effects of metal ions, salts, and toxic compounds; regulators promote the growth and metabolism of the microbial flora; and nutrients promote the growth and reproduction of microorganisms. These substances work together to improve the biosynergist's efficiency in removing ammonia nitrogen and total nitrogen. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other implementation drawings based on the provided drawings without inventive effort.

[0053] Figure 1 This is the infrared spectrum characterization diagram of naphthylacetic acid derivatives. DETAILED DESCRIPTION

[0054] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0055] The following first describes the concepts involved in this application with reference to the accompanying drawings. It should be noted that the following description of each concept is intended only to make the content of this application easier to understand and does not limit the scope of protection of this application. At the same time, the embodiments and features in the embodiments of this application can be combined with each other unless there is a conflict. The following detailed description of this application will be made with reference to the accompanying drawings and in conjunction with the embodiments.

[0056] Example 1: Preparation of biological enhancer: Nitrosomonas, Nitrobacter, Denitrifying Bacteria, Nitrite Reductase, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Mo, Spirulina powder, citric acid, malic acid, lactic acid, glucose, yeast extract and peptone are added to water and mixed to obtain biological enhancer. The mass ratio of Nitrosomonas to Nitrobacter is 1:1, the mass ratio of Nitrosomonas to Denitrifying Bacteria is 1:3, the mass ratio of Nitrosomonas to Nitrite Reductase is 1:1, the mass ratio of Nitrosomonas to EDTA-Fe is 1:0.024, the mass ratio of EDTA-Fe to EDTA-Mn is 1:0.5, the mass ratio of EDTA-Fe to EDTA-Zn is 1:0.5, the mass ratio of EDTA-Fe to EDTA-Mo is The mass ratio of Nitrosomonas to Spirulina powder is 1:0.1, the mass ratio of Nitrosomonas to spirulina powder is 1:0.5, the mass ratio of Nitrosomonas to citric acid is 1:0.017, the mass ratio of citric acid to malic acid is 1:1, the mass ratio of citric acid to lactic acid is 1:1, the mass ratio of Nitrosomonas to glucose is 1:0.4, the mass ratio of glucose to yeast extract is 1:1, the mass ratio of glucose to peptone is 1:0.5, and the mass ratio of Nitrosomonas to water is 1:91.4.

[0057] Example 2: Preparation of naphthylacetic acid derivatives: Add naphthylacetic acid to dichloromethane to obtain a solution. Add N,N'-dicyclohexylcarbodiimide, 4-dimethylaminopyridine, and 4-hydroxy-3-methoxyphenylethanol to the solution and allow to react at 25°C for 10 hours. After completion of the reaction, filter to obtain a filtrate, concentrate the filtrate, add water, and then extract with ethyl acetate. Separate the organic layer and wash it sequentially with acetic acid solution, sodium hydroxide solution, and water. After washing, dry it to obtain the naphthylacetic acid derivative. The usage ratio of naphthylacetic acid to dichloromethane is 1 g:10 ml, the mass ratio of naphthylacetic acid to N,N'-dicyclohexylcarbodiimide is 1:1.2, the mass ratio of naphthylacetic acid to 4-dimethylaminopyridine is 1:0.07, the mass ratio of naphthylacetic acid to 4-hydroxy-3-methoxyphenylethanol is 1:0.9, the usage ratio of naphthylacetic acid to water is 1 g:10 ml, the acetic acid solution consists of acetic acid and water, and the mass ratio of acetic acid to water is 1:19, and the sodium hydroxide solution consists of sodium hydroxide and water, and the mass ratio of sodium hydroxide to water is 1:24.

[0058] Preparation of the biosynergist: Nitrosomonas, Nitrobacter, Denitrifying Bacteria, nitrite reductase, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Mo, spirulina powder, citric acid, malic acid, lactic acid, naphthylacetic acid derivatives, 2-ethyl-3-methyl-quinoline-4-carboxylic acid, glucose, yeast extract and peptone are added into water and mixed to obtain the biosynergist. The mass ratio of Nitrosomonas to Nitrobacter is 1:1, the mass ratio of Nitrosomonas to Denitrifying Bacteria is 1:3, the mass ratio of Nitrosomonas to Nitrite Reductase is 1:1, the mass ratio of Nitrosomonas to EDTA-Fe is 1:0.024, the mass ratio of EDTA-Fe to EDTA-Mn is 1:0.5, the mass ratio of EDTA-Fe to EDTA-Zn is 1:0.5, the mass ratio of EDTA-Fe to EDTA-Mo is 1:0.1, the mass ratio of Nitrosomonas to Spirulina powder is 1:0.5, and the mass ratio of Nitrosomonas to Nitrobacter is 1:1. The mass ratio of Nitrosomonas to citric acid is 1:0.017, the mass ratio of citric acid to malic acid is 1:1, the mass ratio of citric acid to lactic acid is 1:1, the mass ratio of Nitrosomonas to naphthylacetic acid derivatives is 1:0.02, the mass ratio of naphthylacetic acid derivatives to 2-ethyl-3-methyl-quinoline-4-carboxylic acid is 1:1, the mass ratio of Nitrosomonas to glucose is 1:0.4, the mass ratio of glucose to yeast extract is 1:1, the mass ratio of glucose to peptone is 1:0.5, and the mass ratio of Nitrosomonas to water is 1:91.4.

[0059] Example 3: The preparation of naphthylacetic acid derivatives is the same as in Example 2.

[0060] Preparation of biosynergist: The preparation of the biosynergist in this example is compared with that in Example 2, except that the mass ratio of the amount of Nitrosomonas to the naphthacetic acid derivative used is 1:0.03, and other conditions and parameters are the same as in Example 2.

[0061] Example 4: The preparation of naphthylacetic acid derivatives is the same as in Example 2.

[0062] Preparation of biosynergist: The preparation of the biosynergist in this embodiment is compared with that in Example 2, except that the mass ratio of the amount of Nitrosomonas to the naphthacetic acid derivative used is 1:0.009, and other conditions and parameters are the same as in Example 2.

[0063] Example 5: The preparation of naphthylacetic acid derivatives is the same as in Example 2.

[0064] Preparation of the biosynergist: Nitrosomonas, Nitrobacter, Denitrifying Bacteria, nitrite reductase, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Mo, spirulina powder, citric acid, malic acid, lactic acid, naphthylacetic acid derivatives, 2-ethyl-3-methyl-quinoline-4-carboxylic acid, N-acetyl-L-tyrosine ethyl ester, glucose, yeast extract and peptone are added into water and mixed to obtain the biosynergist. The mass ratio of Nitrosomonas to Nitrobacter is 1:1, the mass ratio of Nitrosomonas to Denitrifying Bacteria is 1:3, the mass ratio of Nitrosomonas to Nitrite Reductase is 1:1, the mass ratio of Nitrosomonas to EDTA-Fe is 1:0.024, the mass ratio of EDTA-Fe to EDTA-Mn is 1:0.5, the mass ratio of EDTA-Fe to EDTA-Zn is 1:0.5, the mass ratio of EDTA-Fe to EDTA-Mo is 1:0.1, the mass ratio of Nitrosomonas to Spirulina powder is 1:0.5, the mass ratio of Nitrosomonas to citric acid is 1:0.1, and the mass ratio of Nitrosomonas to spirulina powder is 1:0.5. The mass ratio of citric acid to malic acid is 1:1, the mass ratio of citric acid to lactic acid is 1:1, the mass ratio of Nitrosomonas to naphthylacetic acid derivatives is 1:0.02, the mass ratio of naphthylacetic acid derivatives to 2-ethyl-3-methyl-quinoline-4-carboxylic acid is 1:1, the mass ratio of naphthylacetic acid derivatives to N-acetyl-L-tyrosine ethyl ester is 1:0.7, the mass ratio of Nitrosomonas to glucose is 1:0.4, the mass ratio of glucose to yeast extract is 1:1, the mass ratio of glucose to peptone is 1:0.5, and the mass ratio of Nitrosomonas to water is 1:91.4.

[0065] Example 6: The preparation of naphthylacetic acid derivatives is the same as in Example 2.

[0066] Preparation of biosynergist: The preparation of the biosynergist in this example is compared with that in Example 5, except that the mass ratio of the naphthylacetic acid derivative to N-acetyl-L-tyrosine ethyl ester is 1:1.1. Other conditions and parameters are the same as those in Example 5.

[0067] Comparative Example 1: The preparation of naphthylacetic acid derivatives is the same as in Example 2.

[0068] Preparation of biosynergist: The preparation of the biosynergist in this comparative example is compared with that in Example 2, except that no naphthylacetic acid derivative is used. Other conditions and parameters are the same as those in Example 2.

[0069] Experimental Example 1: The naphthylacetic acid derivatives prepared in Example 2 were characterized by infrared spectroscopy analysis in the scanning range of 500-4000 cm -1 The results are as follows Figure 1 As shown, 3400cm -1 is the absorption peak of -OH hydroxyl group, 3030cm -1 is the absorption peak of benzene ring CH; 2930cm -1 and 2865cm -1 The absorption peaks of -CH3 and -CH2 are 1725cm -1 is the absorption peak of C=O; 1592cm -1 is the absorption peak of C=C; 1260 cm -1 is the absorption peak of COC; 1030 cm -1 is the absorption peak of CO.

[0070] Experimental Example 2: Determination of ammonia nitrogen concentration. Dosage plan: The biosynergists prepared in Examples 1-6 and Comparative Example 1 were added continuously to the wastewater nitrification tank of an integrated circuit company. The dosage was based on the volume of the nitrification tank and the water inflow: 300 mg / L per day for the first 3 days, and 100 mg / L per day for days 4-10. The effluent ammonia nitrogen concentration was 40 mg / L.

[0071] Operation condition control: The overall DO of the system nitrification tank is controlled at above 3mg / L, and the temperature of the biochemical system is controlled at 20℃-38℃ to ensure the stability of various water quality indicators in the system and maintain appropriate sludge age.

[0072] Effect monitoring: Use Nessler's reagent spectrophotometry to detect the ammonia nitrogen concentration in sewage.

[0073] Table 1 Determination results of ammonia nitrogen concentration

[0074] The results are shown in Table 1. After the biosynergist prepared in Example 2 treats sewage, the ammonia nitrogen concentration is lower than that in Example 1, indicating that the use of naphthylacetic acid derivatives and 2-ethyl-3-methyl-quinoline-4-carboxylic acid can improve the degradation ability of the prepared biosynergist on ammonia nitrogen; compared with Example 3, Example 2 shows that increasing the usage of naphthylacetic acid derivatives within a certain range can improve the degradation ability of the prepared biosynergist on ammonia nitrogen and reduce the ammonia nitrogen concentration in sewage; compared with Example 3, Example 2 shows that increasing the usage of naphthylacetic acid derivatives within a certain range can improve the degradation ability of the prepared biosynergist on ammonia nitrogen and reduce the ammonia nitrogen concentration in sewage; compared with Example 4, Example 2 shows that reducing the usage of naphthylacetic acid derivatives within a certain range can improve the degradation ability of the prepared biosynergist on ammonia nitrogen and reduce the ammonia nitrogen concentration in sewage. Will reduce the degradation ability of the prepared biosynergist for ammonia nitrogen; Example 2 is compared with Example 5, indicating that on the basis of using naphthylacetic acid derivatives, 2-ethyl-3-methyl-quinoline-4-carboxylic acid and bacteria, the use of N-acetyl-L-tyrosine ethyl ester can further improve the degradation ability of the prepared biosynergist for ammonia nitrogen and reduce the ammonia nitrogen concentration in sewage; Example 5 is compared with Example 6, indicating that the increase in the usage of N-acetyl-L-tyrosine ethyl ester within a certain range can improve the degradation ability of the prepared biosynergist for ammonia nitrogen; Example 2 is compared with Comparative Example 1, indicating that naphthylacetic acid derivatives need to be used together with 2-ethyl-3-methyl-quinoline-4-carboxylic acid, and too low a usage amount of naphthylacetic acid derivatives will reduce the degradation ability of the prepared biosynergist for ammonia nitrogen.

[0075] Experimental Example 3: Determination of ammonia nitrogen concentration. Dosage plan: The semiconductor company's wastewater is complex with impurities. The bio-enhancers prepared in Examples 1-6 and Comparative Example 1 were added to the semiconductor company's wastewater aerobic tank. The bio-enhancers were added at a rate of 450 mg / L for 10 days. The effluent ammonia nitrogen concentration was 30 mg / L.

[0076] Operation condition control: maintain stable system load, control the DO of the aerobic tank above 2 mg / L, the pH at 7-9, the temperature of the biochemical system at 20℃-35℃, and supplement phosphorus nutrition to ensure that the C:P ratio of the aeration tank inlet water is 100:1.

[0077] Effect monitoring: Use Nessler's reagent spectrophotometry to detect the ammonia nitrogen concentration in sewage.

[0078] Table 2 Determination results of ammonia nitrogen concentration

[0079] The results are shown in Table 2. After the biosynergist prepared in Example 2 treats the sewage, the ammonia nitrogen concentration is lower than that in Example 1, indicating that the use of naphthylacetic acid derivatives and 2-ethyl-3-methyl-quinoline-4-carboxylic acid can cooperate with the bacteria in the biosynergist to improve the degradation ability of the prepared biosynergist for complex wastewater ammonia nitrogen; compared with Example 3, Example 2 shows that the increase in the usage of naphthylacetic acid derivatives within a certain range can improve the degradation ability of the prepared biosynergist for ammonia nitrogen and reduce the ammonia nitrogen concentration in the sewage; compared with Example 3, Example 2 shows that the increase in the usage of naphthylacetic acid derivatives within a certain range can improve the degradation ability of the prepared biosynergist for ammonia nitrogen and reduce the ammonia nitrogen concentration in the sewage; compared with Example 4, Example 2 shows that the usage of naphthylacetic acid derivatives within a certain range can improve the degradation ability of the prepared biosynergist for ammonia nitrogen and reduce the ammonia nitrogen concentration in the sewage. Compared with Example 5, Example 2 shows that on the basis of using naphthylacetic acid derivatives, 2-ethyl-3-methyl-quinoline-4-carboxylic acid and bacteria, the use of N-acetyl-L-tyrosine ethyl ester can further improve the degradation ability of the prepared biosynergist for ammonia nitrogen and reduce the ammonia nitrogen concentration in sewage; Compared with Example 5 and Example 6, it is shown that the improvement of the usage of N-acetyl-L-tyrosine ethyl ester within a certain range can improve the degradation ability of the prepared biosynergist for ammonia nitrogen; Compared with Comparative Example 1, Example 2 shows that naphthylacetic acid derivatives need to be used together with 2-ethyl-3-methyl-quinoline-4-carboxylic acid, and too low a usage of naphthylacetic acid derivatives will reduce the degradation ability of the prepared biosynergist for ammonia nitrogen.

[0080] Experimental Example 4: Determination of total nitrogen concentration. The dosing scheme and operating conditions are the same as those in Experimental Example 2. Effect monitoring: The total nitrogen concentration of the wastewater is detected by UV spectrophotometry using alkaline potassium persulfate digestion, and the total nitrogen removal rate is calculated.

[0081] Table 3 Determination results of total nitrogen removal rate

[0082] The results are shown in Table 3. After the wastewater is treated with the biosynergist prepared in Example 2, the total nitrogen removal rate is lower than that of Example 1, indicating that the use of naphthylacetic acid derivatives and 2-ethyl-3-methyl-quinoline-4-carboxylic acid can improve the total nitrogen removal ability of the prepared biosynergist; compared with Example 3, Example 2 shows that increasing the amount of naphthylacetic acid derivatives within a certain range can improve the total nitrogen removal ability of the prepared biosynergist; compared with Example 3, Example 2 shows that increasing the amount of naphthylacetic acid derivatives within a certain range can improve the total nitrogen removal ability of the prepared biosynergist; compared with Example 4, Example 2 shows that reducing the amount of naphthylacetic acid derivatives within a certain range can reduce the total nitrogen removal ability of the prepared biosynergist. The removal capacity of the prepared biosynergist for total nitrogen; Example 2 is compared with Example 5, indicating that on the basis of using naphthylacetic acid derivatives, 2-ethyl-3-methyl-quinoline-4-carboxylic acid and bacteria, the use of N-acetyl-L-tyrosine ethyl ester can further improve the removal capacity of the prepared biosynergist for total nitrogen; Example 5 is compared with Example 6, indicating that the increase in the usage of N-acetyl-L-tyrosine ethyl ester within a certain range can improve the removal capacity of the prepared biosynergist for total nitrogen; Example 2 is compared with Comparative Example 1, indicating that naphthylacetic acid derivatives need to be used together with 2-ethyl-3-methyl-quinoline-4-carboxylic acid, and too low an amount of naphthylacetic acid derivatives will reduce the removal capacity of the prepared biosynergist for total nitrogen.

[0083] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and do not impose any form of limitation on the implementation methods of the technology of the present invention. Any person skilled in the art may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0084] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression, there are objectively infinite specific structures. For ordinary technicians in this technical field, without departing from the principles of this application, they can also make several improvements, modifications or changes, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. A biosynergist comprising a microbial flora, a functional additive, and a solvent; the microbial flora comprises Nitrosomonas, Nitrobacter, and Denitrifying Bacteria, and the mass proportion of the microbial flora in the biosynergist is 3%-6%.

2. A biosynergist according to claim 1, characterized in that The functional additives include enzyme preparations, trace elements, algae substances, organic acids, regulators and nutrients.

3. A biosynergist according to claim 2, characterized in that: The enzyme preparation comprises at least one of urease, nitrate reductase and nitrite reductase, and the mass proportion of the enzyme preparation in the biosynergist is 1%-2%.

4. A biosynergist according to claim 2, characterized in that: The trace elements include EDTA-Fe, EDTA-Mn, EDTA-Zn and EDTA-Mo, and the mass proportion of the trace elements in the biological enhancer is 0.05%-0.15%.

5. A biosynergist according to claim 2, characterized in that: The algae material is spirulina powder, and the mass proportion of the algae material in the biological enhancer is 0.5%-1%.

6. A biosynergist according to claim 2, characterized in that: The organic acids include citric acid, malic acid and lactic acid, and the mass proportion of the organic acids in the biosynergist is 0.05%-0.1%.

7. A biosynergist according to claim 2, characterized in that: The regulator includes a naphthylacetic acid derivative and 2-ethyl-3-methyl-quinoline-4-carboxylic acid. The naphthylacetic acid derivative is prepared from naphthylacetic acid and 4-hydroxy-3-methoxyphenylethanol. The mass proportion of the regulator in the biosynergist is 0.01%-0.1%.

8. A biosynergist according to claim 2, characterized in that: The nutrients include glucose, yeast extract and peptone, and the mass proportion of the nutrients in the bio-synergist is 1%-2%.

9. A biosynergist according to claim 1, characterized in that: The solvent is water, and the mass proportion of the solvent in the biosynergist is 88.65%-94.39%.

10. Use of the biosynergist according to any one of claims 1 to 9 in the preparation of purified water.

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