Automatic culturing and monitoring device for blueberry somatic embryos
By designing an automated culture and monitoring device for blueberry somatic embryos, the nutrient solution content is monitored and the spraying amount of spray head is controlled, the problem of inaccurate supply of nutrient solution in traditional culture is solved, and efficient blueberry somatic cell culture is achieved.
Patent Information
- Application Number
- CN202510627615.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-15
- Publication Date
- 2025-08-12
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In traditional blueberry somatic embryo culture, it is difficult to achieve accurate differentiation and dynamic adjustment of the supply of nutrient solution, resulting in excessive or insufficient nutrient solution, affecting the development effect, and real-time monitoring and feedback adjustment.
An automated culture and monitoring device for blueberry somatic embryos is designed, including a cultivation stand and a liquid injection mechanism. The nutrient solution content is monitored by the liquid drain mechanism and the spray amount of the spray head is controlled through the flow valve to achieve automated culture and monitoring.
It realizes efficient and synchronous cultivation of blueberry somatic cells, improves the cultivation efficiency, ensures the accuracy and real-time adjustment of nutrient solution supply, and avoids waste or insufficient nutrient solution.
Smart Images
Figure CN120458005A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates specifically to the technical field of blueberry cultivation, in particular to an automated cultivation and monitoring device for blueberry somatic embryos. Background Art
[0002] Blueberries are sweet and sour, rich in nutrients, and have been shown to help prevent brain aging, protect eyesight, strengthen the heart, fight cancer, soften blood vessels, and enhance the immune system. As a highly nutritious commercial crop, blueberry tissue culture technology is crucial for variety improvement and large-scale production.
[0003] During the culture of blueberry somatic embryos, the supply of nutrient solution directly influences the growth and differentiation of the explants. Traditional culture methods typically rely on manual, timed and quantitative addition of nutrient solution, or on simple, timed irrigation systems. However, when culturing multiple culture holes simultaneously, nutrient solution consumption may vary from hole to hole, making it difficult to achieve precise, differentiated supply with traditional methods. Furthermore, nutrient solution requirements vary at different stages of culture, making manual addition difficult to precisely control and easily leading to either excessive or insufficient nutrient solution, thus impacting the development of somatic embryos.
[0004] Existing technologies are usually unable to monitor the nutrient solution content in the culture hole in real time, resulting in the inability to dynamically adjust the supply according to actual needs. Most automated irrigation systems use preset program control and cannot provide feedback and adjustment based on real-time changes in the culture environment, resulting in waste of nutrient solution or insufficient supply. Summary of the Invention
[0005] The object of the present invention is to provide an automated culture and monitoring device for blueberry somatic embryos to solve the problems raised in the above background technology.
[0006] To achieve the above object, the present invention provides the following technical solutions:
[0007] Disclosed is an automated blueberry somatic embryo cultivation and monitoring device, comprising a cultivation rack and a liquid injection mechanism. The cultivation rack comprises a cultivation seat, longitudinal beams, and transverse beams. The cultivation seat is provided with a plurality of cultivation holes for storing nutrient solution and explants. The liquid injection mechanism comprises a base, a flow valve, a liquid storage barrel, and a nozzle. The base is slidably connected to the transverse beam, the liquid storage barrel is fixedly mounted on the base via a support frame, the flow valve is mounted on the base, a discharge end of the liquid storage barrel is connected to the flow valve via a discharge pipe, and the discharge end of the flow is connected to the nozzle.
[0008] As a further solution of the present invention: the longitudinal beams are installed around the cultivation seat, the cross beams are fixedly installed on the upper ends of the longitudinal beams, and two adjacent cross beams are fixedly connected by a connecting beam.
[0009] As a further solution of the present invention: the flow valve includes an upper shell and a lower shell, the upper shell is fixedly mounted on the base, the lower shell is rotatably mounted inside the base, a first flow plate is installed inside the lower shell, and a second flow plate is installed inside the upper shell, and small holes are provided on the first flow plate and the second flow plate.
[0010] As a further solution of the present invention: a top block is slidably mounted on the side of the base, a frame is slidably mounted inside the base, the top block is fixedly connected to the frame, and a rack is fixedly mounted on one side of the frame.
[0011] As a further solution of the present invention: a gear is fixedly installed on the outside of the lower shell, and the gear is engaged with the rack.
[0012] As a further solution of the present invention: it also includes an adjustment mechanism, the adjustment mechanism includes an adjustment screw, both ends of the adjustment screw are rotatably connected to the connecting beam, and the adjustment screw is threadedly connected to the base.
[0013] As a further solution of the present invention: a mounting bracket is fixedly mounted on the connecting beam on one side, a driving motor is provided on the mounting bracket, and an output end of the driving motor is fixedly connected to the adjusting screw.
[0014] As a further solution of the present invention: a liquid drawing mechanism is provided in the cultivation seat, and the liquid drawing mechanism includes a liquid drawing tube installed in the cultivation hole and a plurality of capillaries connected to the liquid drawing tube.
[0015] As a further solution of the present invention: it also includes a connecting tube and a control mechanism, the control mechanism includes a mounting seat and a push rod, the mounting seat is fixedly installed on the cross beam, the push rod is slidably installed on the mounting seat, a slide groove is provided inside the mounting seat, a slider is slidably installed inside the slide groove, and the slider is connected to one end of the slide groove through a connecting spring; the push rod is fixedly connected to the slider, a liquid inlet is provided on one side of the mounting seat, the liquid inlet is communicated with one end of the connecting tube, and the other end of the connecting tube is communicated with the liquid drawing tube.
[0016] As a further solution of the present invention: the push rod is arranged toward the inner side of the measuring hole, and the control mechanism corresponds to the planting hole one by one.
[0017] Compared with the prior art, the present invention has the following beneficial effects: a plurality of cultivation holes are provided in the cultivation seat, nutrient solution and blueberry explants are stored in the cultivation holes, and the blueberry explants are synchronously cultivated through the plurality of planting holes, thereby improving the blueberry cultivation efficiency; a liquid pumping mechanism is provided in the planting hole, and a liquid injection mechanism is provided on the planting rack, and the liquid pumping mechanism is used to monitor the nutrient solution content in the planting hole, and the nutrient solution content is used as a flow valve control signal of the liquid injection mechanism to control the flow valve, thereby adjusting the nutrient solution spraying amount of the nozzle, and realizing automatic monitoring and cultivation of blueberry somatic cells. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 This is a schematic diagram of the structure of the automated culture and monitoring device for blueberry somatic embryos.
[0019] Figure 2 This is an overhead view of the automated culture and monitoring device for blueberry somatic embryos.
[0020] Figure 3 This is a schematic diagram of the structure of the liquid injection mechanism in the automated culture and monitoring device for blueberry somatic embryos.
[0021] Figure 4 This is a side view of the liquid injection mechanism in the automated culture and monitoring device for blueberry somatic embryos.
[0022] Figure 5 This is a schematic diagram of the structure of the base in the automated culture and monitoring device for blueberry somatic embryos.
[0023] Figure 6 This is a schematic diagram of the structure of the flow valve in the automated culture and monitoring device for blueberry somatic embryos.
[0024] Figure 7 This is a schematic diagram of the structure of the control mechanism in the automated culture and monitoring device for blueberry somatic embryos.
[0025] In the figure: 10-cultivation rack, 11-cultivation seat, 12-longitudinal beam, 13-cross beam, 14-connecting beam, 20-adjusting mechanism, 21-adjusting screw, 22-mounting frame, 23-drive motor, 30-liquid injection mechanism, 31-base, 32-support frame, 33-top block, 34-flow valve, 341-lower shell, 342-first flow plate, 343-upper shell, 344-second flow plate, 35-liquid storage barrel, 36-drain pipe, 37-sprinkler, 38-frame, 381-rack, 39-gear, 40-liquid drawing mechanism, 41-liquid drawing pipe, 42-capillary, 50-connecting pipe, 60-control mechanism, 61-mounting seat, 62-top rod, 63-chute, 64-connecting spring, 65-slider, 66-liquid inlet. DETAILED DESCRIPTION
[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0027] Example 1
[0028] See also Figure 1-Figure 7 In an embodiment of the present invention, an automated culture and monitoring device for blueberry somatic embryos includes a culture rack 10 and a liquid injection mechanism 30. The culture rack 10 includes a culture seat 11, a longitudinal beam 12, and a transverse beam 13. The culture seat 11 is provided with a plurality of culture holes, which are used to store nutrient solution and explants. In this embodiment, the explants include the stem tip, leaves, or callus tissue of blueberries. The blueberry somatic cells can be cultured by implanting the explants into the culture holes containing the nutrient solution. The cultivation seat 11 is equipped with the longitudinal beams 12 on all four sides, and the cross beams 13 are fixedly installed on the upper ends of the longitudinal beams 12. The two adjacent cross beams 13 are fixedly connected by connecting beams 14; the liquid injection mechanism 30 includes a base 31, a flow valve 34, a liquid storage barrel 35 and a nozzle 37. The base 31 is slidably connected to the cross beam 13, and the liquid storage barrel 35 is fixedly installed on the base 31 through a support frame 32. The flow valve 34 is installed on the base 31, and the discharge end of the liquid storage barrel 35 is connected to the flow valve 34 through a discharge pipe 36. The discharge end of the flow 34 is connected to the nozzle 37, and the nozzle 37 is installed on the base 31. When the base 31 moves along the cross beam 13, the base 31 adjusts the position of the nozzle 37 so that the nozzle 37 reaches the top of each cultivation hole, and the nozzle 37 is used to replenish the nutrient solution in the cultivation hole to ensure the successful cultivation of blueberry somatic cells.
[0029] In the embodiment of the present application, the flow valve 34 includes an upper shell 343 and a lower shell 341. The upper shell 343 is fixedly mounted on the base 31, and the lower shell 341 is rotatably mounted inside the base 31. A first flow plate 342 is mounted inside the lower shell 341, and a second flow plate 344 is mounted inside the upper shell 343. Small holes are opened on the first flow plate 342 and the second flow plate 344. In the initial state, the small hole on the first flow plate 342 is opposite to the small hole on the second flow plate 344, that is, the small holes of the two flow plates are conductive. The area is at its maximum value. When the lower shell 341 rotates, the small holes on the first flow plate 342 are offset from the small holes on the second flow plate 344, and the conduction area of the small holes of the two flow plates is reduced. When the small holes on the first flow plate 342 are completely offset from the small holes on the second flow plate 344, the conduction area of the small holes of the two flow plates is at its minimum value, which is 0. It can be understood that by controlling the rotation of the lower shell 341 and then controlling the rotation of the first flow plate 342, the conduction area of the small holes of the two flow plates is changed, thereby controlling the discharge amount of the spray 37.
[0030] In the embodiment of the present application, a top block 33 is slidably installed on the side of the base 31, and a frame 38 is slidably installed inside the base 31. The top block 33 is fixedly connected to the frame 38, and a rack 381 is fixedly installed on one side of the frame 38; a gear 39 is fixedly installed on the outside of the lower shell 341, and the gear 39 is engaged with the rack 381. When the top block 33 and the frame 38 are displaced on the base 31, the rack 381 controls the rotation of the gear 39, thereby controlling the rotation of the lower shell 341.
[0031] In the embodiment of the present application, an adjustment mechanism 20 is also included. The adjustment mechanism 20 includes an adjustment screw 21. The two ends of the adjustment screw 21 are rotatably connected to the connecting beam 14, and the adjustment screw 21 is threadedly connected to the base 31. A mounting bracket 22 is fixedly mounted on one side of the connecting beam 14. The mounting bracket 22 is provided with a drive motor 23. The output end of the drive motor 23 is fixedly connected to the adjustment screw 21. The drive motor 23 is used to control the rotation of the adjustment screw 21, thereby adjusting the sliding position of the base 31. In this embodiment, the drive motor 23 is a forward and reverse motor structure. The drive motor 23 can control the reciprocating motion of the base 31 along the beam 13 through the adjustment screw 21.
[0032] Example 2
[0033] See also Figure 1-Figure 7In an embodiment of the present invention, an automated culture and monitoring device for blueberry somatic embryos includes a culture rack 10 and a liquid injection mechanism 30. The culture rack 10 includes a culture seat 11, a longitudinal beam 12, and a transverse beam 13. The culture seat 11 is provided with a plurality of culture holes, which are used to store nutrient solution and explants. In this embodiment, the explants include the stem tip, leaves, or callus tissue of blueberries. The blueberry somatic cells can be cultured by implanting the explants into the culture holes containing the nutrient solution. The cultivation seat 11 is equipped with the longitudinal beams 12 on all four sides, and the cross beams 13 are fixedly installed on the upper ends of the longitudinal beams 12. The two adjacent cross beams 13 are fixedly connected by connecting beams 14; the liquid injection mechanism 30 includes a base 31, a flow valve 34, a liquid storage barrel 35 and a nozzle 37. The base 31 is slidably connected to the cross beam 13, and the liquid storage barrel 35 is fixedly installed on the base 31 through a support frame 32. The flow valve 34 is installed on the base 31, and the discharge end of the liquid storage barrel 35 is connected to the flow valve 34 through a discharge pipe 36. The discharge end of the flow 34 is connected to the nozzle 37, and the nozzle 37 is installed on the base 31. When the base 31 moves along the cross beam 13, the base 31 adjusts the position of the nozzle 37 so that the nozzle 37 reaches the top of each cultivation hole, and the nozzle 37 is used to replenish the nutrient solution in the cultivation hole to ensure the successful cultivation of blueberry somatic cells.
[0034] In the embodiment of the present application, the flow valve 34 includes an upper shell 343 and a lower shell 341. The upper shell 343 is fixedly mounted on the base 31, and the lower shell 341 is rotatably mounted inside the base 31. A first flow plate 342 is mounted inside the lower shell 341, and a second flow plate 344 is mounted inside the upper shell 343. Small holes are opened on the first flow plate 342 and the second flow plate 344. In the initial state, the small hole on the first flow plate 342 is opposite to the small hole on the second flow plate 344, that is, the small holes of the two flow plates are conductive. The area is at its maximum value. When the lower shell 341 rotates, the small holes on the first flow plate 342 are offset from the small holes on the second flow plate 344, and the conduction area of the small holes of the two flow plates is reduced. When the small holes on the first flow plate 342 are completely offset from the small holes on the second flow plate 344, the conduction area of the small holes of the two flow plates is at its minimum value, which is 0. It can be understood that by controlling the rotation of the lower shell 341 and then controlling the rotation of the first flow plate 342, the conduction area of the small holes of the two flow plates is changed, thereby controlling the discharge amount of the spray 37.
[0035] In the embodiment of the present application, a top block 33 is slidably installed on the side of the base 31, and a frame 38 is slidably installed inside the base 31. The top block 33 is fixedly connected to the frame 38, and a rack 381 is fixedly installed on one side of the frame 38; a gear 39 is fixedly installed on the outside of the lower shell 341, and the gear 39 is engaged with the rack 381. When the top block 33 and the frame 38 are displaced on the base 31, the rack 381 controls the rotation of the gear 39, thereby controlling the rotation of the lower shell 341.
[0036] In the embodiment of the present application, an adjustment mechanism 20 is also included. The adjustment mechanism 20 includes an adjustment screw 21. The two ends of the adjustment screw 21 are rotatably connected to the connecting beam 14, and the adjustment screw 21 is threadedly connected to the base 31. A mounting bracket 22 is fixedly mounted on one side of the connecting beam 14. The mounting bracket 22 is provided with a drive motor 23. The output end of the drive motor 23 is fixedly connected to the adjustment screw 21. The drive motor 23 is used to control the rotation of the adjustment screw 21, thereby adjusting the sliding position of the base 31. In this embodiment, the drive motor 23 is a forward and reverse motor structure. The drive motor 23 can control the reciprocating motion of the base 31 along the beam 13 through the adjustment screw 21.
[0037] The difference between Example 2 and Example 1 is that:
[0038] The cultivation seat 11 is provided with a liquid drawing mechanism 40, and the liquid drawing mechanism 40 includes a liquid drawing tube 41 installed in the cultivation hole and a plurality of capillaries 42 connected to the liquid drawing tube 42; it should be noted that after the nutrient solution inside the planting hole flows into the capillary tube 42, the capillary force can cause the liquid around the wet tube wall to rise naturally. In this embodiment, the capillary force is used to draw the nutrient solution inside the planting hole, and the liquid amount of the nutrient solution inside the planting hole can be monitored through the capillary tube 42. When the liquid amount of the nutrient solution inside the planting hole is large, the capillary effect is more obvious. In this embodiment, the capillary tube 42 is a capillary tube made of hydrophilic material, specifically a glass fiber tube or a nano-ceramic tube.
[0039] In the embodiment of the present application, a connecting tube 50 and a control mechanism 60 are further included. The control mechanism 60 includes a mounting seat 61 and a push rod 62. The mounting seat 61 is fixedly mounted on the crossbeam 13, and the push rod 62 is slidably mounted on the mounting seat 61. A slide groove 63 is defined inside the mounting seat 61, and a slider 65 is slidably mounted inside the slide groove 63. The slider 65 is connected to one end of the slide groove 63 via a connecting spring 64; the push rod 62 is fixedly connected to the slider 65, and a liquid inlet 66 is defined on one side of the mounting seat 61. The liquid inlet 66 is communicated with one end of the connecting tube 50, and the other end of the connecting tube 50 is communicated with the liquid drawing tube 41. When the liquid in the liquid drawing tube 41 increases, the liquid squeezes the push rod 62, causing the push rod 62 to move on the mounting seat 61.
[0040] It should be noted that in the embodiment of the present application, the push rod 62 is arranged toward the inner side of the measure 13, and the control mechanism 60 corresponds one-to-one to the planting hole. During the movement of the injection mechanism 30, when the push block 33 contacts the displaced push rod 62, the push rod 62 squeezes the push block 33, so that the push block 33 controls the movement of the gear 39 through the rack 381, and then controls the rotation of the lower shell 341 of the flow valve 34, so that the flow valve 34 reduces the liquid inlet amount of the nozzle 37, thereby avoiding the increase of nutrient solution inside the planting hole.
[0041] In summary, the present invention is provided with multiple cultivation holes in the cultivation base, and the cultivation holes are used to store nutrient solution and blueberry explants. The blueberry explants are synchronously cultivated through the multiple planting holes, thereby improving the blueberry cultivation efficiency; the present invention is provided with a liquid suction mechanism in the planting hole, and a liquid injection mechanism is also provided on the planting rack. The liquid suction mechanism is used to monitor the nutrient solution content in the planting hole, and the nutrient solution content is used as a flow valve control signal of the liquid injection mechanism to control the flow valve, adjust the nutrient solution spraying amount of the nozzle, and realize automatic monitoring and cultivation of blueberry somatic cells.
[0042] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.
[0043] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.
Claims
1. A blueberry somatic embryo automated culture and monitoring device, characterized in that: The invention comprises a cultivation frame (10) and a liquid injection mechanism (30), wherein the cultivation frame (10) comprises a cultivation seat (11), a longitudinal beam (12) and a transverse beam (13), wherein a plurality of cultivation holes are provided in the cultivation seat (11), and the cultivation holes are used to store nutrient solution and explants; the liquid injection mechanism (30) comprises a base (31), a flow valve (34), a liquid storage barrel (35) and a nozzle (37), wherein the base (31) is slidably connected to the transverse beam (13), the liquid storage barrel (35) is fixedly mounted on the base (31) through a support frame (32), the flow valve (34) is mounted on the base (31), the discharge end of the liquid storage barrel (35) is communicated with the flow valve (34) through a discharge pipe (36), and the discharge end of the flow (34) is communicated with the nozzle (37).
2. The automated culture and monitoring device for blueberry somatic embryos according to claim 1, characterized in that: The longitudinal beams (12) are installed around the cultivation seat (11), the cross beams (13) are fixedly installed on the upper ends of the longitudinal beams (12), and two adjacent cross beams (13) are fixedly connected by a connecting beam (14).
3. The automated culture and monitoring device for blueberry somatic embryos according to claim 2, characterized in that: The flow valve (34) includes an upper shell (343) and a lower shell (341), wherein the upper shell (343) is fixedly mounted on the base (31), and the lower shell (341) is rotatably mounted inside the base (31), a first flow plate (342) is mounted inside the lower shell (341), and a second flow plate (344) is mounted inside the upper shell (343), and small holes are provided on both the first flow plate (342) and the second flow plate (344).
4. The automated culture and monitoring device for blueberry somatic embryos according to claim 3, characterized in that: A top block (33) is slidably mounted on the side of the base (31), a frame (38) is slidably mounted inside the base (31), the top block (33) is fixedly connected to the frame (38), and a rack (381) is fixedly mounted on one side of the frame (38).
5. The automated culture and monitoring device for blueberry somatic embryos according to claim 4, characterized in that: A gear (39) is fixedly mounted on the outside of the lower housing (341), and the gear (39) is meshed with the rack (381).
6. The automated culture and monitoring device for blueberry somatic embryos according to claim 1, characterized in that: The invention also includes an adjusting mechanism (20), wherein the adjusting mechanism (20) includes an adjusting screw (21), both ends of the adjusting screw (21) are rotatably connected to the connecting beam (14), and the adjusting screw (21) is threadedly connected to the base (31).
7. The automated culture and monitoring device for blueberry somatic embryos according to claim 6, characterized in that: A mounting frame (22) is fixedly mounted on the connecting beam (14) on one side, a driving motor (23) is provided on the mounting frame (22), and an output end of the driving motor (23) is fixedly connected to the adjusting screw (21).
8. The automated culture and monitoring device for blueberry somatic embryos according to claim 1, characterized in that: The cultivation seat (11) is provided with a liquid drawing mechanism (40), and the liquid drawing mechanism (40) comprises a liquid drawing tube (41) installed in the cultivation hole and a plurality of capillaries (42) connected to the liquid drawing tube (42).
9. The automated culture and monitoring device for blueberry somatic embryos according to claim 8, characterized in that: The invention also includes a connecting pipe (50) and a control mechanism (60). The control mechanism (60) includes a mounting seat (61) and a push rod (62). The mounting seat (61) is fixedly mounted on the cross beam (13). The push rod (62) is slidably mounted on the mounting seat (61). A sliding groove (63) is provided inside the mounting seat (61). A slider (65) is slidably mounted inside the sliding groove (63). The slider (65) is connected to one end of the sliding groove (63) through a connecting spring (64). The push rod (62) is fixedly connected to the slider (65). A liquid inlet (66) is provided on one side of the mounting seat (61). The liquid inlet (66) is communicated with one end of the connecting pipe (50). The other end of the connecting pipe (50) is communicated with the liquid drawing pipe (41).
10. The automated culture and monitoring device for blueberry somatic embryos according to claim 9, characterized in that: The push rod (62) is arranged toward the inner side of the measuring rod (13), and the control mechanism (60) corresponds to the planting hole one by one.