Acaricidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide mite killing, and discloses a mite killing composition and application thereof.The mite killing composition comprises an active ingredient A and an active ingredient B. The active ingredient A is sulfiflumin, and the active ingredient B is any one of fluazinam and pyriminostrobin. The acaricidal composition disclosed by the invention has a remarkable synergistic effect of preventing and treating phytophagous harmful mites such as panonychus citri, effectively reduces the use dosage of pesticides, and is safe to the environment.

Description

[0001] This invention application is a divisional application with application number 202311764473.4, application date December 21, 2023, and invention name “A mite-killing composition and its application”. Technical Field

[0002] The invention belongs to the technical field of pesticide acaricide and discloses an acaricide composition and application thereof. Background Art

[0003] Fluazinam, CAS number 79622-59-6, chemical name 3-chloro-N-[3-chloro-2,6-dinitro-4-(trifluoromethyl)phenyl]-5-(trifluoromethyl)pyridin-2-amine, is an oxidative phosphorylation uncoupler with excellent control effects against plant diseases such as early and late blight, and also has some control effects against mites.

[0004] Mefenamic acid, CAS registration number 1257598-43-8, chemical name is (E)-2-[2-[[2-(2,4-dichloroanilino)-6-(trifluoromethyl)pyrimidin-4-yl]oxymethyl]phenyl]-3-methoxyprop-2-enoic acid methyl ester, is a mitochondrial respiratory chain inhibitor.

[0005] Herbivorous mites are closely related to agricultural production. Their diverse species, wide distribution, and polyphagous nature make them a recognized pest group that is difficult to control. These pests primarily belong to the Tetranychus family. Adults, nymphs, and larvae of these mites suck sap from the front or back of plant leaves, increasing transpiration, reducing chlorophyll, and inhibiting photosynthesis. This leads to leaf discoloration, dryness, and, in severe cases, the death of the entire plant.

[0006] Agricultural production has long relied primarily on chemical control to control large-scale mite outbreaks. The unique ecological strategies and environmental adaptability of mites have led to an increasing problem of pesticide resistance. The extensive use of pesticides also exacerbates environmental pollution and disrupts the natural ecological balance. Therefore, the applicants have combined sulfiflumin with either fluazinam or pyrimidifen and found that the combination has a significant synergistic effect on the control of herbivorous mites, slowing the development of pesticide resistance and ensuring environmental safety. Summary of the Invention

[0007] Based on the above, the object of the present invention is to provide a mite-killing composition. The mite-killing composition of the present invention has a significant synergistic effect in controlling phytophagous pest mites such as citrus mites, effectively reduces the dosage of pesticides, and is safe for the environment.

[0008] Another object of the present invention is to provide a mite-killing composition that is safe for crops;

[0009] In order to achieve the above-mentioned object, the present invention adopts the following technical solution: an acaricidal composition, wherein the active ingredients of the acaricidal composition include active ingredient A and active ingredient B, the active ingredient A is sulfiflumin, the active ingredient B is either fluazinam or pyrimidifen, and the mass ratio of the active ingredient to the active ingredient B is 1:30 to 42:1.

[0010] Furthermore, the active ingredient B in the acaricidal composition is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 42:1, such as 1:30, 1:24, 1:12, 1:6, 1:3, 6:1, 10:1, 24:1, 35:1, 42:1 or any value between the above values;

[0011] Furthermore, the active ingredient B in the acaricidal composition is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 35:1, such as 1:24, 1:12, 1:6, 1:3, 6:1, 10:1, 24:1, 35:1 or any value in between;

[0012] Furthermore, the active ingredient B in the acaricidal composition is pyrimethamine, and the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 25:1, such as 1:28, 1:15, 2:9, 2:3, 3:2, 6:1, 12:1, 25:1 or any value between the above values;

[0013] Furthermore, the active ingredient B in the acaricidal composition is pyrimethamine, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 12:1, such as 1:15, 2:9, 2:3, 3:2, 6:1, 12:1 or any value between the above values;

[0014] Furthermore, the acaricidal composition further comprises, in addition to the active ingredient, auxiliary ingredients acceptable in pesticides, wherein the auxiliary ingredients are wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, preservatives, stabilizers, synergists, fillers, solvents or carriers;

[0015] Furthermore, the wetting agent is one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0016] Furthermore, the dispersant is one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0017] Furthermore, the emulsifier is one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0018] Furthermore, the thickener is one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0019] Furthermore, the disintegrant is one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0020] Furthermore, the antifreeze agent is one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0021] Furthermore, the defoaming agent is C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0022] Furthermore, the preservative is one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0023] Furthermore, the stabilizer is one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0024] Furthermore, the synergist is selected from synergist phosphine and piperonyl butoxide; and / or

[0025] Furthermore, the filler is a solvent, a filler or a carrier;

[0026] Furthermore, the solvent is one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0027] Furthermore, the carrier is one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0028] Furthermore, the formulation of the acaricide composition is in the form of a soluble solution, a suspension concentrate, an emulsifiable concentrate, an aqueous emulsion, a microcapsule suspension, a microemulsion, a suspoemulsion, a dispersible oil suspension, a granule, a water-dispersible granule, or a wettable powder;

[0029] Furthermore, the formulation is in the form of a suspension, emulsifiable concentrate, water-dispersible granules, or wettable powder.

[0030] The present invention also discloses the use of the acaricidal composition for preventing and controlling herbivorous mites.

[0031] Furthermore, the phytophagous pest mites are Tetranychus pest mites.

[0032] Furthermore, the pest mite of the Tetranychus family is Panonychus citri, Panonychus apicale, Tetranychus cinnabarinus or Tetranychus urticae.

[0033] Furthermore, the acaricidal composition is applied to the harmful mites, the plants where they grow, or the environment where they grow.

[0034] The beneficial effects of the present invention are as follows:

[0035] 1) The acaricidal composition of the present invention has a significant synergistic effect in controlling herbivorous mites at a certain ratio, especially having a good control effect on citrus mites;

[0036] 2) The acaricidal composition of the present invention can significantly reduce the population of harmful mites, reduce the damage caused by harmful mites to crops, improve the rapid effect of the agent, and delay the development of drug resistance;

[0037] 3) The acaricidal composition of the present invention is safe and highly effective, and is safe to crops, non-target organisms, beneficial organisms and natural enemies. DETAILED DESCRIPTION

[0038] The following will be combined with the preparation examples and specific embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0039] Preparation Example 1: 28% sulfiflumin·fluazinam water dispersible granules (1:6)

[0040] Formula composition: 4% sulfiflumin, 24% fluazinam, 10% naphthalenesulfonic acid formaldehyde polymer sodium salt, 2% polycarboxylic acid sodium salt, 4% open powder BX, kaolin makes up the balance;

[0041] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and surfactants and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and screening are carried out to obtain a water-dispersible granule product; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0042] Preparation Example 2: 18% sulfiflumin·fluazinam suspension (8:1)

[0043] Formula composition: 16% sulfiflumin, 2% fluazinam, 2% alkylphenol polyoxyethylene ether, 1% polycarboxylic acid sodium salt, 4% alkylphenol polyoxyethylene ether phosphate salt, 2% alkylaryl polyoxyethylene ether polyoxypropylene ether, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 4% ethylene glycol, 0.1% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0044] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0045] Preparation Example 3: 22% sulfiflumin·pyrimidine suspension (2:9)

[0046] Formula composition: 4% sulfiflumin, 18% pyrimidifen, 2% glycerol fatty acid ester polyoxyethylene ether, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% naphthalenesulfonate formaldehyde condensate, 5% polyoxyethylene sorbitan monooleate, 1% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.4% silicone oil, and deionized water to make up the balance;

[0047] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0048] Preparation Example 4: 21% sulfiflumin·pyrimidifen water dispersible granules (6:1)

[0049] Formula composition: 18% sulfiflumin, 3% pyrimidifen, 8% dispersant MF-5, 2% sodium polycarboxylate, 2% sodium lauryl sulfate, and light calcium carbonate to make up the balance.

[0050] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and surfactants and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and screening are carried out to obtain a water-dispersible granule product; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0051] Preparation Example 5: 15% sulfiflumin·pyrimidifen wettable powder (2:3)

[0052] Formula composition: 6% sulfiflumin, 9% cypermethrin, 10% calcium lignin sulfonate, 2% dispersant NNO, 2% sodium lauryl sulfate, and kaolin to make up the balance;

[0053] Preparation method: According to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0054] Example 1: Indoor biotoxicity activity assay

[0055] Test basis: The test refers to NY / T1154.13-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 13: Leaf Disk Spray Method".

[0056] Biological test material: Citrus red spider (Panonychus citri).

[0057] Test agents: sulfiflumin technical, fluazinam technical, and pyrimidifen technical.

[0058] Experimental preparation: Select citrus leaves with uniform growth and no pesticides, use a hole punch to make leaf discs, place a wet sponge in a culture dish, put filter paper on it, and put leaf discs on the filter paper, 2 leaf discs per dish, inoculate the test mites on the leaf discs, 15 mites per leaf disc.

[0059] Preparation of pharmaceutical preparation: Prepare the original drug into a mother liquor with an organic solvent, and then use a 0.1% Tween 80 aqueous solution to prepare 5 series of mass concentrations in equal proportions.

[0060] Chemical treatment: Place the Petri dish on the bottom plate of a potter spray tower and spray with a volume of 1 mL. Allow the chemical to settle for 1 minute before removing the dish and transferring the fish to normal conditions for rearing. Each treatment was replicated four times, with a treatment containing no chemical (including all organic solvents and emulsifiers) serving as a blank control.

[0061] Rearing and observation: The treated test mites were reared and observed at a temperature of (25±1)°C and a photoperiod of L:D=(16:8)h.

[0062] Inspection: 48 hours after treatment, check the mortality of test insects and record the total number of mites and the number of dead mites respectively.

[0063] Calculation method:

[0064] Based on the survey data, calculate the mortality rate of each treatment using the following formula:

[0065]

[0066] Where:

[0067] P——mortality rate, in percentage (%);

[0068] K——indicates the number of dead insects, the unit is head;

[0069] N——represents the total number of insects processed, in heads.

[0070]

[0071] Where:

[0072] P1——adjusted mortality rate, in percentage (%);

[0073] P t ——Treatment mortality rate, expressed in percentage (%);

[0074] P0 - blank control mortality rate, in percentage (%).

[0075] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0076] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4), and formula (5):

[0077]

[0078] Where:

[0079] ATI - measured toxicity index of mixture;

[0080] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0081] M——LC of the mixture 50, the unit is milligrams per liter (mg / L).

[0082] TTI=TI A ×P A +TI B ×P B

[0083] Where:

[0084] TTI – Theoretical Toxicity Index of Mixtures;

[0085] TI A ——Agent toxicity index;

[0086] P A ——The percentage of agent A in the mixture, in percentage (%);

[0087] TI B ——Toxicity index of agent B;

[0088] P B ——The percentage of agent B in the mixture, in percentage (%).

[0089]

[0090] Where:

[0091] CTC – Co-toxicity coefficient;

[0092] ATI - measured toxicity index of mixture;

[0093] TTI - Theoretical Toxicity Index of Mixture.

[0094] A co-toxicity coefficient (CTC) of ≥120 indicates a synergistic effect; a CTC of ≤80 indicates an antagonistic effect; and a CTC of 80 < CTC <120 indicates an additive effect. The results of the indoor toxicity test are shown in the table below:

[0095] Table 1 Results of indoor bioactivity assays of sulfiflumin and fluazinam against Panonychus citri

[0096] Test drug Virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient Sulfiflumin(A) y=4.3996+1.1979x 0.9864 3.1714 / Fluazinam (B) y=3.3178+1.2794x 0.9952 20.6438 / A:B=1:30 y=3.5771+1.2794x 0.9919 12.9476 135.381 A:B=1:24 y=3.6477+1.3110x 0.9966 10.7529 157.315 A:B=1:12 y=3.6724+1.4325x 0.9968 8.4477 171.634 A:B=1:6 y=4.0083+1.2429x 0.9941 6.2789 183.979 A:B=1:3 y=4.1927+1.2235x 0.9954 4.5691 190.050 A:B=6:1 y=4.6808+1.3639x 0.9943 1.7141 210.466 A:B=10:1 y=4.6223+1.3663x 0.9897 1.8899 181.796 A:B=24:1 y=4.6108+1.2646x 0.9980 2.0312 161.605 A:B=35:1 y=4.6044+1.1026x 0.9941 2.2847 142.152 A:B=42:1 y=4.5167+1.1738x 0.9811 2.5807 125.357 A:B=55:1 y=4.4469+1.1477x 0.9915 3.0336 106.147

[0097] Table 2 Results of indoor bioactivity test of sulfiflumin and pyrimidifen against Panonychus citri

[0098]

[0099]

[0100] As shown in the indoor test results in Table 1-2, the acaricidal composition of the present invention has a good control effect on Panonychus citri. When sulphimid is combined with fluazinam in a mass ratio of 1:30 to 42:1, a synergistic effect is exhibited against Panonychus citri. At a mass ratio of 1:24 to 35:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect. At a mass ratio of 1:12 to 10:1, the co-toxicity coefficient is greater than 170, indicating a significant synergistic effect. At a mass ratio of 55:1, an additive effect is exhibited.

[0101] When Sulfiflumin and pyrimidifen are combined, the mass ratio of the two is 1:28 to 25:1, which shows a synergistic effect; when the mass ratio is 1:15 to 12:1, the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious; when the mass ratio is 1:15 to 6:1, the co-toxicity coefficient is greater than 140, and the synergistic effect is significant; and when the mass ratio is 1:42 and 30:1 to 40:1, it shows an additive effect.

[0102] Example 2: Field control test of citrus red spider mites

[0103] Experimental site: Citrus orchard in Huangyan District, Taizhou City, Zhejiang Province. The crops planted in the experimental site are Wenzhou mandarin oranges. The experimental site is severely damaged by citrus red spider mites all year round. All experimental plots are uniform and consistent, which is in line with local scientific agricultural practices.

[0104] Experimental plot setup: Five chemical treatments and one water control were set up. Each plot was randomly arranged in blocks, with two citrus trees per treatment plot, and each treatment was replicated four times.

[0105] Application Time and Method: Conventional canopy spraying was used with a knapsack sprayer. The test was conducted on May 15, 2023, during normal weather conditions, with no adverse weather conditions that could have affected the results. The test agent was diluted with water according to the designed dosage. Application was thorough and even, with the spray applied until the leaves were dripping.

[0106] Survey schedule and methods: Base population counts were conducted before treatment, followed by four surveys of remaining live mites at 3, 15, and 30 days after treatment. In each plot, one treetop was marked on the east, south, west, north, and center sides of each tree. The top five leaves of each treetop were surveyed, for a total of 50 leaves per plot. The number of live mites was then counted.

[0107] Calculation method of drug efficacy:

[0108]

[0109] Field efficacy test results and analysis:

[0110]

[0111] Three days after application, the results showed that the compounded acaricidal composition of the present invention had an 87.87% and 87.50% efficacy against citrus spider mites, both higher than the single-dose controls. Fifteen days after application, the efficacy of the four agents, 18% sulfiflumin·fluazinam suspension concentrate (8:1) and 15% sulfiflumin·pyrimidine wettable powder (2:3), was 93.46% and 92.60%, respectively. Thirty days after application, the acaricidal composition of the present invention exhibited significantly higher efficacy against citrus spider mites than the single-dose controls, with a prolonged duration of effect. Within the tested concentration range, the acaricidal composition of the present invention did not cause significant phytotoxicity to citrus leaves, young shoots, or fruit.

[0112] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, characterized in that: The active ingredients of the acaricidal composition include active ingredient A and active ingredient B, the active ingredient A is sulfiflumin, the active ingredient B is pyrimidifen, and the mass ratio of the active ingredient to the active ingredient B is 1:30 to 42:

1.

2. The acaricidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:28 to 25:

1.

3. The acaricidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:15 to 12:

1.

4. The acaricidal composition according to claim 1, characterized in that In addition to the active ingredients, the acaricide composition also includes auxiliary ingredients acceptable in pesticides, and the auxiliary ingredients are fillers and / or surfactants.

5. The acaricidal composition according to claim 1, characterized in that The formulation of the acaricide composition is a soluble solution, a suspension, an emulsifiable concentrate, an aqueous emulsion, a microcapsule suspension, a microemulsion, a suspoemulsion, a dispersible oil suspension, a granule, a water-dispersible granule or a wettable powder.

6. The acaricidal composition according to claim 5, characterized in that The preparation dosage form is suspension, emulsifiable concentrate, water-dispersible granules or wettable powder.

7. Use of the acaricidal composition according to any one of claims 1 to 6 for controlling phytophagous pest mites.

8. The use according to claim 7, characterized in that The herbivorous pest mites are of the family Tetranychus.

9. The use according to claim 8, characterized in that The harmful mites of the Tetranychus family are Panonychus citri, Panonychus porcellus, Tetranychus cinnabarinus or Tetranychus urticae.

10. The use according to claim 7, characterized in that The acaricidal composition is applied to harmful mites, plants where they grow, or environments where they grow.

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