High-solubility beta-1, 3 / alpha-1, 3-glucan composition and preparation method thereof

Abstract

The invention provides a high-solubility beta-1, 3 / alpha-1, 3-glucan composition, which is prepared from the following raw materials in parts by weight: 1 to 3 parts of beta-1, 3 / alpha-1, 3-glucan, and 1 to 3 parts of resistant dextrin or fructo-oligosaccharide. The invention also provides a preparation method of the composition. The industrial problem that the dissolution rate of beta-glucan in a compound system is limited is solved. A specific compound raw material combination is screened and a particle size-dissolution synergistic model is established, the synergistic enhancement effect of resistant dextrin and fructo-oligosaccharide on beta-1, 3 / alpha-1, 3-glucan dissolution is found for the first time, meanwhile, a compound raw material grading granulation-particle size matching technology is innovatively proposed, and the dissolution speed of a compound system is remarkably increased (P is smaller than 0.05). According to the method, the technical bottleneck of slow dissolution speed of a compound product is overcome while the biological activity of the beta-1, 3 / alpha-1, 3-glucan is maintained.

Description

Technical Field

[0001] The present invention relates to a high-solubility beta-1,3 / alpha-1,3-glucan composition and a preparation method thereof, and belongs to the field of functional food and medicine. Background Art

[0002] As a functional polysaccharide with multiple biological activities such as lowering blood lipids and regulating immunity, β-glucan is widely used in food and health care products.

[0003] Although the currently available β-1,3 / α-1,3-glucan has achieved full water solubility, there is still a bottleneck in dissolution rate in actual applications, especially in scenarios such as solid beverages and instant preparations that require rapid dissolution. In market applications, β-glucan often needs to be compounded with other raw materials, and β-1,3 / α-1,3-glucan is a new and original glucan, different from common glucans from other sources. Therefore, there are few studies on the dissolution rate of β-1,3 / α-1,3-glucan compounded with other raw materials. Traditional dissolution improvement methods are mostly limited to the physical modification of a single raw material, and do not fully consider key factors such as particle size matching and surface property synergy between the components in the compound system, resulting in poor actual application effects.

[0004] Patent application number: CN202311018357.8, invention name: A method for increasing the dissolution rate of glucan, through the expansion technology, the fluffiness of the glucan powder is increased, the contact surface of the glucan powder and the aqueous solution is increased, and rapid dissolution is achieved. Patent application number: CN201811456882.7, invention name: Solid dispersion of β-glucan and preparation method thereof, discloses a solid dispersion of β-glucan and a preparation method thereof. The method obtains a solid dispersion by compounding β-glucan with a high-viscosity polymer. Among them, the selected high-viscosity polymer includes xanthan gum, konjac gum, guar gum, locust bean gum, carrageenan, sodium carboxymethyl cellulose, or a combination of these substances. Using this method, the solubility characteristics of β-glucan are improved to a certain extent. The raw material targeted by the above document is β-glucan.

[0005] β-1,3 / α-1,3-glucan is a novel polysaccharide composed of seven β-1,3-D-glucose units and two α-1,3-D-glucose units linked by glycosidic bonds, forming a repeating chain structure without side chains. This unique structure gives it distinct properties from other traditional β-glucans. β-1,3 / α-1,3-glucan is typically produced through a bio-fermentation process. For example, β-1,3 / α-1,3-glucan is fermented using Rhizobium pusense with sucrose as the primary raw material. The product is then produced through a series of processes including alcohol precipitation, filtration, separation, drying, and pulverization. This production method ensures its naturalness and high purity, with the β-1,3 / α-1,3-glucan content typically exceeding 90g / 100g.

[0006] Patent application number: CN202411401225.8, Invention Title: A Method for Improving the Dissolution Rate of β-1,3 / α-1,3-Glucan, only screens and determines a single optimal particle size range for improving the dissolution rate of β-1,3 / α-1,3-glucan, and does not involve compounding with other raw materials, which has limitations in market application. Patent application number: CN202411572531.8, Invention Title: A Solid Dispersion of β-1,3 / α-1,3-Glucan, Preparation Method, and Application thereof, focuses on preparing a solid dispersion of β-1,3 / α-1,3-glucan and other polysaccharides to improve the stability of beverages, but does not explore its dissolution rate. Patent application number: CN202411572532.2, invention name: A solid dispersion of β-1,3 / α-1,3-glucan and its preparation method and application, discloses a solid dispersion formed by a composite of β-1,3 / α-1,3-glucan and phosphate, mainly targeting plant protein beverages, and the main focus is on reducing the viscosity of the beverage.

[0007] Currently reported technologies for improving the solubility of β-1,3 / α-1,3-glucan have limitations. First, modification methods are designed only for a single raw material system and fail to consider the synergistic solubility effects of different raw materials in compounding scenarios. Second, while puffing improves dissolution speed, it results in a product with too low a bulk density, potentially affecting packaging and transportation economics. The method of adding high-viscosity polymers to compound to produce a solid dispersion has less than ideal improvement effects, and the resulting solid dispersion has extremely high viscosity, which severely restricts its widespread practical application. Summary of the Invention

[0008] The present invention solves the industry problem of limited dissolution rate of β-glucan in compound systems.

[0009] The present invention provides a high-solubility β-1,3 / α-1,3-glucan composition, which is prepared from the following raw materials in the following weight ratio:

[0010] 1-3 parts of β-1,3 / α-1,3-glucan, 1-2 parts of resistant dextrin or oligofructose.

[0011] Preferably, it is prepared from the following raw materials in the following weight ratio:

[0012] 1 part β-1,3 / α-1,3-glucan, 2 parts resistant dextrin; or

[0013] 3 parts of β-1,3 / α-1,3-glucan and 1 part of resistant dextrin; or

[0014] 3 parts of β-1,3 / α-1,3-glucan and 1 part of oligofructose.

[0015] The composition of the present invention is prepared by wet granulating the raw materials of beta-1,3 / alpha-1,3-glucan, resistant dextrin or oligofructose, passing the granules through a 30-mesh sieve, mixing the granules and drying the granules.

[0016] The present invention provides a method for preparing the highly soluble β-1,3 / α-1,3-glucan composition, which comprises the following steps:

[0017] a. Weigh the raw materials of each weight ratio;

[0018] b. The raw materials were wet granulated using 50% ethanol as a binder;

[0019] c. Sieve through a 30-mesh sieve, mix, and dry.

[0020] The sieving method is to first pass the particles through a 60-mesh sieve to remove oversized particles, and then pass the particles through a 30-mesh sieve to collect the particles.

[0021] Wherein, the drying method described in step c is a drying method.

[0022] By screening specific compound raw material combinations and establishing a particle size-dissolution synergistic model, researchers discovered for the first time the synergistic enhancement effect of resistant dextrin and oligofructose on the dissolution of β-1,3 / α-1,3-glucan. Furthermore, they innovatively proposed a "graded granulation of compound raw materials - particle size matching" technique, significantly improving the dissolution rate of the compound system (P < 0.05). This approach overcomes the technical bottleneck of slow dissolution of compound products while maintaining the biological activity of β-1,3 / α-1,3-glucan. DETAILED DESCRIPTION

[0023] Example 1 Screening test of the present invention's prescription

[0024] 1. Raw material formula

[0025] Compound raw materials: resistant dextrin, galacto-oligosaccharide, fructo-oligosaccharide, polydextrose

[0026] Functional ingredient: β-1,3 / α-1,3-glucan

[0027] 2. Granulation

[0028] (1) Granulation

[0029] The compound raw materials of resistant dextrin, galacto-oligosaccharide, fructo-oligosaccharide, polydextrose and the functional ingredient β-1,3 / α-1,3-glucan were respectively made into granules by wet granulation method with 50% edible alcohol as a binder.

[0030] (2) Screening and drying

[0031] First, pass the mixture through a 60-mesh sieve to remove oversized particles, then pass through a 30-mesh sieve to collect the particles. Dry the particles in an oven to a constant weight.

[0032] 3. Solubility test

[0033] Experimental group:

[0034] A: β-1,3 / α-1,3-glucan: resistant dextrin = 1:1 (β-1,3 / α-1,3-glucan 1.00g, resistant dextrin 1.00g)

[0035] B: β-1,3 / α-1,3-glucan: galacto-oligosaccharide = 1:1 (β-1,3 / α-1,3-glucan 1.00g, galacto-oligosaccharide 1.00g)

[0036] C: β-1,3 / α-1,3-glucan: oligofructose = 1:1 (β-1,3 / α-1,3-glucan 1.00g, oligofructose 1.00g)

[0037] D: β-1,3 / α-1,3-glucan: polydextrose = 1:1 (β-1,3 / α-1,3-glucan 1.00g, polydextrose 1.00g)

[0038] Control group:

[0039] E: β-1,3 / α-1,3-glucan: β-1,3 / α-1,3-glucan = 1:1 (β-1,3 / α-1,3-glucan 1.00g, β-1,3 / α-1,3-glucan 1.00g)

[0040] Place the above combinations into beakers respectively, add 50 ml of water, place on a magnetic stirrer, stir at the same speed, and measure the time for complete dissolution.

[0041] 4. Test results

[0042] Table 1 Dissolution time of different compound formulas

[0043]

[0044]

[0045] (p<0.05)

[0046] As can be seen from Table 1, the dissolution rate of β-1,3 / α-1,3-glucan particles can be significantly improved after being compounded with resistant dextrin or oligofructose (P < 0.05).

[0047] Example 2 Screening test for the optimal ratio of the composition of the present invention

[0048] According to the experimental results of Example 1, the effects of different ratios of resistant dextrin, oligofructose and β-1,3 / α-1,3-glucan on the dissolution rate were investigated.

[0049] 1. Test formula

[0050] A: β-1,3 / α-1,3-glucan: resistant dextrin = 1:1 (β-1,3 / α-1,3-glucan 1.00g, resistant dextrin 1.00g)

[0051] B: β-1,3 / α-1,3-glucan: resistant dextrin = 2:1 (β-1,3 / α-1,3-glucan 1.33g, resistant dextrin 0.67g)

[0052] C: β-1,3 / α-1,3-glucan: resistant dextrin = 3:1 (β-1,3 / α-1,3-glucan 1.50g, resistant dextrin 0.50g)

[0053] D: β-1,3 / α-1,3-glucan: resistant dextrin = 1:2 (β-1,3 / α-1,3-glucan 0.67g, resistant dextrin 1.33g)

[0054] E: β-1,3 / α-1,3-glucan: resistant dextrin = 1:3 (β-1,3 / α-1,3-glucan 0.50g, resistant dextrin 1.50g)

[0055] F: β-1,3 / α-1,3-glucan: oligofructose = 1:1 (β-1,3 / α-1,3-glucan 1.00g, oligofructose 1.00g)

[0056] G: β-1,3 / α-1,3-glucan: oligofructose = 2:1 (β-1,3 / α-1,3-glucan 1.33g, oligofructose 0.67g)

[0057] H: β-1,3 / α-1,3-glucan: oligofructose = 3:1 (β-1,3 / α-1,3-glucan 1.50g, oligofructose 0.50g)

[0058] I: β-1,3 / α-1,3-glucan: oligofructose = 1:2 (β-1,3 / α-1,3-glucan 0.67g, oligofructose 1.33g)

[0059] J: β-1,3 / α-1,3-glucan: oligofructose = 1:3 (β-1,3 / α-1,3-glucan 0.50g, oligofructose 1.50g)

[0060] Control group:

[0061] K: β-1,3 / α-1,3-glucan: β-1,3 / α-1,3-glucan = 1:1 (β-1,3 / α-1,3-glucan 1.00g, β-1,3 / α-1,3-glucan 1.00g)

[0062] Place the above combinations into beakers respectively, add 50 ml of water, place on a magnetic stirrer, stir at the same speed, and measure the time for complete dissolution.

[0063] Experimental results:

[0064] Table 2 Dissolution time of different compound formulas

[0065]

[0066] (p<0.05)

[0067] 3. Results Analysis

[0068] According to the results of difference analysis, when compounded with resistant dextrin, the dissolution rate can be significantly improved when the ratio of β-1,3 / α-1,3-glucan:resistant dextrin is 1:2 and β-1,3 / α-1,3-glucan:resistant dextrin is 3:1 (P < 0.05).

[0069] When compounded with oligofructose, the dissolution rate can be significantly improved when β-1,3 / α-1,3-glucan: oligofructose = 3:1 (P < 0.05).

Claims

1. A highly soluble β-1,3 / α-1,3-glucan composition, characterized by: It is prepared from the following raw materials in the following weight ratio: 1-3 parts of β-1,3 / α-1,3-glucan, 1-3 parts of resistant dextrin or oligofructose.

2. The highly soluble β-1,3 / α-1,3-glucan composition according to claim 1, characterized in that: It is prepared from the following raw materials in the following weight ratio: 1 part β-1,3 / α-1,3-glucan, 2 parts resistant dextrin; or 3 parts of β-1,3 / α-1,3-glucan and 1 part of resistant dextrin; or 3 parts of β-1,3 / α-1,3-glucan and 1 part of oligofructose.

3. The highly soluble β-1,3 / α-1,3-glucan composition according to claim 1 or 2, characterized in that: The preparation method comprises the following steps: wet-granulating the raw materials beta-1,3 / alpha-1,3-glucan, resistant dextrin or oligofructose, passing the granules through a 30-mesh sieve, mixing the granules and drying the granules.

4. A method for preparing the highly soluble β-1,3 / α-1,3-glucan composition according to any one of claims 1 to 3, characterized in that: It includes the following steps: a. Weigh the raw materials of each weight ratio; b. The raw materials were wet granulated using 50% ethanol as a binder; c. Sieve through a 30-mesh sieve, mix, and dry.

5. The method for preparing a highly soluble β-1,3 / α-1,3-glucan composition according to claim 4, wherein: The screening method is to first pass the particles through a 60-mesh sieve to remove oversized particles, and then pass the particles through a 30-mesh sieve to collect the particles.

6. The method for preparing a highly soluble β-1,3 / α-1,3-glucan composition according to claim 4, wherein: The drying method described in step c is a drying method.

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