Acupoint-sticking plaster for preventing and treating calf diarrhea, oral compound combined medicine and application

Abstract

The invention discloses an acupoint-sticking plaster for preventing and treating calf diarrhea, an oral compound combined medicine and application, and belongs to the technical field of veterinary medicine compositions.The combined medicine comprises a traditional Chinese veterinary medicine acupoint-sticking plaster and an oral compound, and the traditional Chinese veterinary medicine acupoint-sticking plaster comprises extract of Xinjiang cardamom; the oral compound comprises the following components: stewed radix aucklandiae, wine-processed betel nut, wine-processed rheum officinale and fructus evodiae; the cure rate of the combined medicine on calf diarrhea caused by multi-germ and multi-virus composite infection is 88.3%, and the effective rate reaches 100%; the combined medicine can be used for clinically treating calf diarrhea caused by multi-virus and multi-germ compound infection, and has an important practical application value in clinical treatment of veterinary calf diarrhea in a large-scale cattle farm.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary drug compositions, and in particular relates to an acupuncture plaster for preventing and treating calf diarrhea and an oral compound medicine and applications thereof. Background Art

[0002] Calf diarrhea is a common disease in beef and dairy cattle farming, with a high incidence rate. It is one of the leading causes of mortality in cattle, severely impacting the healthy development of the dairy and beef cattle industries. Its complex etiology makes it recognized worldwide as one of the greatest challenges in beef and dairy farming. Calf diarrhea causes dehydration, acidosis, and electrolyte imbalance in calves, leading to clinical symptoms such as lethargy, loss of appetite, watery, loose, or soft stools, vomiting, dehydration, and weight loss. It can occur year-round, but is most common in the cold winter and early spring.

[0003] Common ones include bovine viral diarrhea virus (BVDV), bovine rotavirus (BRV), bovine coronavirus (BCoV) and bovine enterovirus (BEV); bacterial infections such as Escherichia coli, Salmonella, Shigella coli, Proteus mirabilis, and Clostridium perfringens are also common causes of calf diarrhea.

[0004] Therefore, developing a non-resistant Chinese herbal medicine combination drug with good efficacy against calf diarrhea is of great significance for beef and dairy cattle breeding. Summary of the Invention

[0005] In view of the above problems, the present invention discloses an acupuncture plaster for preventing and treating calf diarrhea and an oral compound medicine and its application.

[0006] The present invention adopts the following technical solutions:

[0007] Disclosed are an acupuncture plaster and an oral compound medicine for preventing and treating calf diarrhea. The compound medicine comprises a Chinese veterinary acupuncture plaster and an oral compound. The components of the Chinese veterinary acupuncture plaster include an extract of Xinjiang mung bean; the components of the oral compound include stewed costus root, wine-processed betel nut, wine-processed rhubarb and rutaecarpa.

[0008] Furthermore, the extract concentrate is obtained by extraction using an ethanol solution with a concentration of 70%-80%.

[0009] Furthermore, the extraction weight ratio of the Xinjiang green cardamom to the ethanol solution is 1:10-20.

[0010] Furthermore, the extract concentrate is an extract concentrate obtained by concentrating the extract of the ethanol solution.

[0011] Furthermore, the extract is applied to a square rubber patch and sterilized by ultraviolet light to obtain the Chinese veterinary acupuncture plaster.

[0012] Furthermore, the weight ratio of the medicinal components of the oral compound is: 3-10 parts of stewed costus root, 6-10 parts of wine-processed betel nut, 3-10 parts of wine-processed rhubarb and 3-9 parts of evodia rutaecarpa.

[0013] Furthermore, the weight ratio of the medicinal components of the oral compound is: 5-7 parts of stewed costus root, 7-9 parts of wine-processed betel nut, 5-7 parts of wine-processed rhubarb and 5-7 parts of evodia rutaecarpa.

[0014] Furthermore, the weight ratio of the medicinal components of the oral compound is: 6.5 parts of stewed costus root, 8.5 parts of wine-processed betel nut, 6.5 parts of wine-processed rhubarb and 6.5 parts of Evodia rutaecarpa.

[0015] Furthermore, the stewed costus root, wine-processed betel nut, wine-processed rhubarb and Evodia rutaecarpa are ground into fine powder and passed through a 60-mesh sieve.

[0016] An application of the above-mentioned combined medicine, wherein the acupuncture plaster and the oral compound are simultaneously used for preventing and treating calf diarrhea.

[0017] Beneficial effects:

[0018] The Chinese veterinary acupuncture plaster and internal compound combination medicine for preventing and treating calf diarrhea disclosed in the present invention can achieve the combined therapeutic effect of external acupuncture plaster stimulation and internal medicine prescriptions, can significantly improve the self-immunity of diarrheal calves, and has the advantages of simple operation, no drug resistance, multi-target effect, and low cost. The cure rate of the Chinese veterinary medicine for calf diarrhea caused by multi-pathogen and multi-virus complex infection is 88.3%, and the effective rate is 100%. The combined application of the acupuncture plaster and the internal compound combination drug of the present invention has a significant comprehensive therapeutic effect on calf diarrhea caused by multi-virus and multi-pathogen infection; analysis of viral and pathogen detection results before and after treatment shows that the traditional Chinese veterinary medicine acupuncture plaster and internal compound combination for preventing and treating calf diarrhea has a very significant inhibitory effect on Proteus mirabilis, Clostridium perfringens and BRV, BVDV, BCV, and BNoV viruses; analysis of blood routine changes before and after treatment shows that the newly developed traditional Chinese veterinary medicine acupuncture plaster and internal compound combination drug for preventing and treating calf diarrhea have the functions of preventing excessive loss of body fluids in diarrheal calves, reducing the damage of pathogenic factors to the body's coagulation function, regulating the body's immunity, and eliminating the body's mucosal bleeding and microthrombosis function, and have no side effects; the traditional Chinese veterinary medicine acupuncture plaster and internal compound combination drug for preventing and treating calf diarrhea of the present invention have a good therapeutic effect on calf diarrhea caused by multi-pathogen and multi-virus combined infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1This is a schematic diagram of a fecal sample before treatment with the combined drug for preventing and treating calf diarrhea according to the present invention;

[0020] Figure 2 This is a schematic diagram of fecal samples after treatment with the combined drug for preventing and treating calf diarrhea according to the present invention;

[0021] Figure 3 This is a schematic diagram of the detection results of five viruses before and after treatment with the combined drug for preventing and treating calf diarrhea of the present invention;

[0022] Figure 4 A schematic diagram of the acupoints of cattle muscles used in traditional Chinese veterinary medicine for acupuncture plasters in the combination medicine for preventing and treating calf diarrhea of the present invention;

[0023] Figure 5 This is a schematic diagram of the Chinese veterinary medicine acupuncture plaster in the combined medicine for preventing and treating calf diarrhea of the present invention. DETAILED DESCRIPTION

[0024] In order to better understand the technical solution of the present invention, the embodiments of the present invention are described in detail below in conjunction with the embodiments.

[0025] It should be understood that the embodiments described are only a portion of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative work are within the scope of protection of the present invention.

[0026] Example 1

[0027] The present invention relates to a combination drug treatment effect judgment and related experimental methods.

[0028] (1) Determination of the overall therapeutic effect of the combined drug of the present invention.

[0029] Weaned diarrhea calves weighing 100-150 kg and aged 4-6 months were selected as the treatment experimental subjects.

[0030] The treatment effect was judged by comparing the feces condition, diarrhea frequency and mental state of the cattle before and after treatment. After treatment, if the feces condition of the cattle returned to normal and the mental state was normal, it was judged to be cured. After treatment, if the feces condition of the cattle improved, the mental state improved, and the diarrhea frequency decreased, it was judged to be effective. After treatment, if the diarrhea frequency of the cattle did not change and the cattle still had diarrhea and the mental state did not improve, it was judged to be ineffective.

[0031] (2) Comparative treatment experiments of acupoint application alone, oral administration of compound medicine alone, and acupoint application and oral administration combined.

[0032] Thirty weaned calves with diarrhea, weighing 100-150 kg and aged 4-6 months, were selected and randomly divided into three groups, with 10 calves in each group; one group was treated with acupuncture therapy alone, one group was treated with oral compound alone, and one group was treated with acupuncture therapy and oral compound combination treatment; the differences in fecal status, mental state, and defecation frequency of the cattle were observed after the individual and combined treatments.

[0033] (3) Routine blood test method.

[0034] Non-anticoagulated blood samples were collected from calves with diarrhea before and after treatment with the drug of the present invention, and on-site analysis was performed using an automatic blood routine analyzer. The following 20 indicators were measured in the calves before and after treatment: red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width index: RDWSD, RDWCV, platelet parameters: platelet count PLT, mean platelet volume MPV, mean platelet hematocrit PCT, platelet distribution width PDW, large platelet ratio P-LCR, and inflammatory cell parameters in the blood: white blood cell parameters WBC, lymphocyte count LYM, monocyte count MON, granulocyte count GRA, lymphocyte percentage LYM%, monocyte percentage MON%, and granulocyte percentage GRA%.

[0035] (4) Bacteria determination method.

[0036] On a clean bench, rinse the anal swab in sterile saline. Mix the solution thoroughly and inoculate it into prepared sterile nutrient broth. Incubate at 37°C for 24–36 hours for bacterial enrichment. When the solution becomes turbid, inoculate it into eosin-methylene blue medium and incubate at 37°C for 18 hours. Select individual colonies characteristic of Escherichia coli Shigella, Proteus mirabilis, Salmonella, and Clostridium perfringens and inoculate them onto MacConkey medium. Incubate at 37°C for an additional 18–24 hours. Gram stain microscopy and biochemical identification are performed.

[0037] (5) Virus detection methods.

[0038] According to the clinical symptoms and reference to the common viruses of calf diarrhea, bovine coronavirus (BCoV), bovine rotavirus (BRV), bovine norovirus (BNoV), bovine viral diarrhea / mucosal disease virus (BVDV), and bovine newb virus (BNeV) of diarrheal calves, multiple pairs of specific primers for detecting pathogens were designed; the collected serum was subjected to viral gene RNA / DNA extraction and PCR amplification according to the kit; anal swabs were vortex-mixed with PBS buffer (1:5), centrifuged at 10,000 rpm for 10 minutes, the supernatant was taken, cultured and separated with pathogen culture medium, and pathogen genomic DNA was extracted according to the kit and conventional PCR amplification was performed; the amplified products were subjected to 1% agarose gel electrophoresis and detected using a gel imaging scanner and compared with the positive control to determine the positive changes in the virus in the anal swab samples before and after treatment with the Chinese herbal compound of the present invention. The basic information of the primers used for detecting the virus is shown in Table 1. The multiple pairs of specific primers for the pathogens were all provided and synthesized by Sangon Biotech (Shanghai) Co., Ltd.

[0039] Table 1 Basic information of primers used for virus detection

[0040]

[0041]

[0042] (6) Data statistics and analysis methods.

[0043] The data were expressed as mean ± standard deviation (mean ± SD), and the independent sample T test was used to analyze the differences in the changes of routine blood indices and positive rates of virus pathogens in anal swabs of diarrheal calves before and after treatment with the Chinese herbal compound of the present invention using SPSS 19.0 statistical software.

[0044] The invention discloses an acupuncture plaster and an internal compound medicine for preventing and treating calf diarrhea. The medicine combination comprises a traditional Chinese veterinary medicine acupuncture plaster and an internal compound. The components of the traditional Chinese veterinary medicine acupuncture plaster include an extract of Xinjiang mung bean; the components of the internal compound include stewed costus root, wine-processed betel nut, wine-processed rhubarb and evodia rutaecarpa.

[0045] Furthermore, the extract concentrate is obtained by extraction using an ethanol solution with a concentration of 70%-80%.

[0046] Furthermore, the extraction weight ratio of the Xinjiang green cardamom to the ethanol solution is 1:10-20.

[0047] Furthermore, the extract concentrate is an extract concentrate obtained by concentrating the extract of the ethanol solution.

[0048] Furthermore, the extract is applied to a square rubber patch and sterilized by ultraviolet light to obtain the Chinese veterinary acupuncture plaster.

[0049] Furthermore, the weight ratio of the medicinal components of the oral compound is: 3-10 parts of stewed costus root, 6-10 parts of wine-processed betel nut, 3-10 parts of wine-processed rhubarb and 3-9 parts of evodia rutaecarpa.

[0050] Furthermore, the weight ratio of the medicinal components of the oral compound is: 5-7 parts of stewed costus root, 7-9 parts of wine-processed betel nut, 5-7 parts of wine-processed rhubarb and 5-7 parts of evodia rutaecarpa.

[0051] Furthermore, the weight ratio of the medicinal components of the oral compound is: 6.5 parts of stewed costus root, 8.5 parts of wine-processed betel nut, 6.5 parts of wine-processed rhubarb and 6.5 parts of Evodia rutaecarpa.

[0052] Furthermore, the stewed costus root, wine-processed betel nut, wine-processed rhubarb and Evodia rutaecarpa are ground into fine powder and passed through a 60-mesh sieve.

[0053] An application of the above-mentioned combined medicine, wherein the Chinese veterinary medicine acupuncture plaster and the internal medicine compound are simultaneously used for preventing and treating calf diarrhea.

[0054] The present invention locates the "Tongqiao", "Pishu" and "Liuma" points in the latissimus dorsi acupuncture points of the Ren and Du meridians and their collaterals of the calf with diarrhea, takes "Pishu" as the center point, removes the hair covering the "Tongqiao", "Pishu" and "Liuma" areas, and applies the Chinese veterinary medicine acupuncture plaster (Xinjiang small green cardamom plaster) of the present invention; at the same time, simmered costus root, wine-processed betel nut, wine-processed rhubarb and Evodia rutaecarpa are selected to develop an internal medicine compound for calf diarrhea; the Chinese veterinary medicine acupuncture plaster is applied to the acupuncture points of the calf with diarrhea, and the internal medicine compound is fed at the same time, combining external treatment with internal treatment, conducting treatment experiments, and observing the efficacy. According to the compatibility, Chinese medicinal slices that meet the requirements of the pharmacopoeia are purchased, Xinjiang small green cardamom is extracted with an ethanol solution, the extract is concentrated to prepare an extract extract, and then the extract extract is used to prepare a plaster; regarding the internal medicine compound: simmered costus root, wine-processed betel nut, wine-processed rhubarb and Evodia rutaecarpa are mixed according to weight ratio, ground into fine powder and passed through a 60-mesh sieve. 20 g of the powder was suspended in normal saline and then gavage was administered once in the morning and evening to weaned calves with diarrhea aged 4 to 6 months. At the same time, the hair covering the "Tongqiao", "Pishu" and "Liumai" areas was removed, and the Chinese veterinary medicine acupuncture plaster (Xinjiang Mung Bean Plaster) of the present invention was applied for 3 consecutive days. Blood samples and anal swabs of the diarrheal calves were collected before and after treatment, and blood routine, virus and bacteria tests were performed, and comparative analysis was performed.

[0055] The acupoint area for external application of the veterinary acupoint plaster in the present invention is selected, and the acupoint area for diarrhea calves is selected by locating the "Tongqiao", "Pishu" and "Liuma" acupoint group in the latissimus dorsi muscle of diarrhea calves; with "Pishu" as the center point, the "Tongqiao", "Pishu" and "Liuma" areas are covered.

[0056] Example 2

[0057] Thirty weaned calves with diarrhea, weighing 100-150 kg and aged 4-6 months, were selected and randomly divided into three groups, with 10 calves in each group; one group was treated with Chinese veterinary medicine acupuncture plaster alone (acupuncture plaster alone group), one group was treated with oral compound alone (oral administration alone group), and one group was treated with a combination of Chinese veterinary medicine acupuncture plaster and oral compound (acupuncture plaster and oral administration combination group).

[0058] Purchase Chinese herbal medicine pieces that meet pharmacopoeia requirements. Veterinary acupuncture plaster: Extract Xinjiang Mung Root with 70% ethanol to create an extract concentrate, which is then prepared as a plaster. For oral administration, combine 5 parts stewed costus root, 7 parts wine-processed betel nut, 5 parts wine-processed rhubarb, and 5 parts evodia rutaecarpa, grind into a fine powder, and pass through a 60-mesh sieve.

[0059] Treatment with Chinese veterinary medicine acupuncture plaster: remove the hair covering the "Tongqiao", "Pishu" and "Liumai" areas, and apply the Chinese veterinary medicine acupuncture plaster of the present invention; oral compound treatment: suspend 20g of the powder in normal saline and then gavage it to 4-6 month old weaned calves with diarrhea once in the morning and evening.

[0060] Three groups of calves with diarrhea were treated with Chinese veterinary medicine acupuncture plaster alone (acupuncture plaster alone group), Chinese veterinary medicine compound for oral administration alone (acupuncture plaster alone group), and a combination of acupuncture plaster and compound for oral administration (acupuncture plaster and compound for oral administration group) for 3 consecutive days. The efficacy was observed. The results of the efficacy of acupuncture plaster alone, oral administration alone, and acupuncture plaster and compound for oral administration are shown in Table 2. The fecal status before and after treatment was as follows: Figure 1 、 Figure 2 shown.

[0061] Table 2 Observation of efficacy of single and combined use

[0062]

[0063] By comparing the therapeutic effects of acupuncture alone, oral administration alone, and the combination of acupuncture and oral administration on calf diarrhea, it was found that the combination of acupuncture and oral administration can quickly improve the mental state of sick cows, quickly reduce the frequency of defecation in diarrheal cows, and restore diarrheal calves to normal within three days.

[0064] Example 3

[0065] 43 weaned diarrhea calves weighing 100-150 kg and aged 4-6 months were selected.

[0066] Choose Chinese herbal medicine slices that meet the requirements of the pharmacopoeia. Chinese veterinary medicine acupuncture plaster: Extract Xinjiang green cardamom with 80% ethanol solution to obtain an extract, and prepare it into a plaster, such as Figure 5 As shown; Oral compound: Take 7 parts of stewed costus root, 9 parts of wine-processed betel nut, 7 parts of wine-processed rhubarb, and 7 parts of Evodia rutaecarpa, mix them, grind them into fine powder and pass through a 60-mesh sieve.

[0067] Treatment with Chinese veterinary medicine acupuncture plaster: remove the hair covering the "Tongqiao", "Pishu" and "Liumai" areas, and apply the Chinese veterinary medicine acupuncture plaster of the present invention, such as Figure 4 As shown; Oral compound treatment: 20g of drug powder is suspended in normal saline and then gavage is given to 4-6 month old weaned calves with diarrhea once in the morning and evening.

[0068] Treatment was conducted continuously for three days using acupuncture plasters and a compound formula for internal use. External and internal treatments were combined in a therapeutic experiment to observe efficacy. Before and after treatment, non-anticoagulated blood was collected from the cows for prompt blood counts and serum separation, which was then stored at -20°C. Disposable sterile anal swabs were used to obtain feces from the anus. The feces were placed in cryopreservation tubes, two per cow, with three swabs per tube, and stored in liquid nitrogen.

[0069] According to the above scheme, 43 calves with diarrhea were treated with the powder after being suspended in physiological saline. After the treatment with the newly developed veterinary drug compound for the prevention and treatment of calf diarrhea, the fecal morphology of 38 cows returned to normal, their mental state was normal, and the treatment result was cured; after treatment, the fecal morphology of 5 cows improved, and their mental state improved compared with before treatment, and the treatment result was effective; therefore, the cure rate of the prescription with the above medicinal material component weight ratio was 88.3% (38 / 43), and the effective rate was 100% (43 / 43).

[0070] Example 4

[0071] 46 weaned diarrhea calves weighing 100-150 kg and aged 4-6 months were selected.

[0072] Purchase Chinese herbal medicine pieces that meet pharmacopoeia requirements. Veterinary acupuncture plaster: Extract Xinjiang Mung Root with 75% ethanol to create an extract concentrate, which is then prepared as a plaster. For oral administration, combine 6.5 parts of stewed costus root, 8.5 parts of wine-processed betel nut, 7 parts of wine-processed rhubarb, and 7 parts of evodia rutaecarpa, grind into a fine powder, and pass through a 60-mesh sieve.

[0073] Treatment with Chinese veterinary medicine acupuncture plaster: remove the hair covering the "Tongqiao", "Pishu" and "Liumai" areas, and apply the Chinese veterinary medicine acupuncture plaster of the present invention; oral compound treatment: suspend 20g of the powder in normal saline and then gavage it to 4-6 month old weaned calves with diarrhea once in the morning and evening.

[0074] Treatment was conducted continuously for three days using acupuncture plasters and a compound formula for internal use. External and internal treatments were combined in a therapeutic experiment to observe efficacy. Before and after treatment, non-anticoagulated blood was collected from the cows for prompt blood counts and serum separation, which was then stored at -20°C. Disposable sterile anal swabs were used to obtain feces from the anus. The feces were placed in cryopreservation tubes, two per cow, with three swabs per tube, and stored in liquid nitrogen.

[0075] According to the above scheme, 46 calves with diarrhea were treated with the powder after being suspended in physiological saline and then gavage-fed. After treatment, the fecal morphology of 41 cows returned to normal and their mental state was normal, and the treatment result was cured; after treatment, the fecal morphology of 5 cows improved and their mental state improved compared with before treatment, and the treatment result was effective; therefore, the cure rate of the prescription with the above medicinal material component weight ratio was 89.1% (41 / 46), and the effective rate was 100% (46 / 46).

[0076] Example 5

[0077] 46 weaned diarrhea calves weighing 100-150 kg and aged 4-6 months were selected.

[0078] Purchase Chinese herbal medicine pieces that meet pharmacopoeia requirements. Veterinary acupuncture plaster: Extract Xinjiang Mung Bean with 75% ethanol to create an extract concentrate, which is then prepared as a plaster. For oral administration, combine 6.5 parts of stewed costus root, 8.5 parts of wine-processed betel nut, 6.5 parts of wine-processed rhubarb, and 6.5 parts of evodia rutaecarpa, grind into a fine powder, and pass through a 60-mesh sieve.

[0079] Treatment with Chinese veterinary medicine acupuncture plaster: remove the hair covering the "Tongqiao", "Pishu" and "Liumai" areas, and apply the Chinese veterinary medicine acupuncture plaster of the present invention; oral compound treatment: suspend 20g of the powder in normal saline and then gavage it to 4-6 month old weaned calves with diarrhea once in the morning and evening.

[0080] Treatment was conducted continuously for three days using acupuncture plasters and a compound formula for internal use. External and internal treatments were combined in a therapeutic experiment to observe efficacy. Before and after treatment, non-anticoagulated blood was collected from the cows for prompt blood counts and serum separation, which was then stored at -20°C. Disposable sterile anal swabs were used to obtain feces from the anus. The feces were placed in cryopreservation tubes, two per cow, with three swabs per tube, and stored in liquid nitrogen.

[0081] According to the above scheme, 46 calves with diarrhea were treated with the powder after being suspended in physiological saline. After treatment, the fecal morphology of 43 cows returned to normal and their mental state was normal, and the treatment results were cured; after treatment, the fecal morphology of 2 cows improved and their mental state improved compared with before treatment, and the treatment results were effective; therefore, the cure rate of the prescription with the above medicinal component weight ratio was 93.5% (43 / 46), and the effective rate was 100% (46 / 46).

[0082] Example 6

[0083] The bacteria in calf feces and viruses in serum, i.e., blood routine tests, were compared before and after treatment with the veterinary acupuncture plaster combined with the oral compound of the present invention. The following test result data are the comparison of the tests on the calves before and after treatment in Example 4. Figure 1 、 Figure 2 Schematic diagram comparing calf feces before and after treatment. It can be seen from the figure that the calf feces returned to normal after treatment.

[0084] According to the bacterial detection method (4) in Example 1, bacteria in cattle feces were detected before and after treatment.

[0085] After bacterial culture of anal swabs of diarrheal calves, Shigella coli, Proteus mirabilis, and Clostridium perfringens were detected. After treatment with the Chinese herbal compound of the present invention, the positive rates of Proteus mirabilis and Clostridium perfringens were extremely significantly reduced compared with before treatment (p=0.000), Salmonella was not detected, and the positive rate of Shigella coli and Escherichia coli did not decrease significantly. The results are shown in Table 3.

[0086] Table 3 Results of pathogen detection before and after treatment with Chinese veterinary medicine acupuncture plaster combined with oral compound (n=46)

[0087]

[0088] According to the virus detection method (5) in Example 1, the virus in the bovine serum before and after treatment was detected.

[0089] Viral RNA was extracted and subjected to gel electrophoresis. The test results of bovine coronavirus (BCoV), bovine rotavirus (BRV), bovine norovirus (BNoV), bovine viral diarrhea / mucosal disease virus (BVDV), and bovine Newbu virus (BNeV) in calves with diarrhea are shown in Table 4 below. The positive rates of BCoV, BRV, BNoV, and BVDV after treatment with the veterinary acupuncture plaster combined with the oral compound of the present invention were significantly reduced (p=0.000) compared with those before treatment, and there was no significant difference in the positive rate of BNeV before and after treatment. Gel electrophoresis of each virus is shown in Table 4 below. Figure 3 .

[0090] Table 4 Virus detection results before and after treatment with veterinary acupuncture plaster combined with oral compound (n=46)

[0091]

[0092]

[0093] According to the blood routine test method (3) in Example 1, the blood routine of the cattle before and after treatment was tested. The blood routine test results are shown in Table 5 below.

[0094] Table 5 Blood routine before and after treatment with veterinary acupuncture plaster combined with oral compound (n=46, mean±SD)

[0095]

[0096]

[0097] Regarding red blood cell (RBC) indices, HGB was significantly lower after treatment compared to before treatment (P<0.05). Regarding platelet indices, P-LCR was significantly lower after treatment compared to before treatment (P<0.05). The remaining blood routine indices showed no significant changes before and after treatment (P>0.05).

[0098] Analysis of the above-mentioned bacterial, viral and blood routine test results.

[0099] RBC, HGC, and HCT change in a correlated manner when severe vomiting, diarrhea, extensive burn tissue fluid exudation, and acidosis occur, reflecting changes in circulating blood volume. When plasma volume decreases due to fluid loss, the relative volume of red blood cells increases, leading to increases in RBC, HGC, and HCT. After treatment with Chinese medicine, the RBC, HGC, and HCT values of calves with diarrhea decreased, with hemoglobin significantly decreasing (P=0.041). This indicates that the combination of the veterinary acupuncture plaster and the internal compound of the present invention can prevent excessive fluid loss in calves with diarrhea. Red blood cell MCV, MCH, and MCHC directly reflect changes in red blood cell morphology and the likelihood of anemia during the course of the disease. When a person is malnourished due to a long-term wasting disease, MCV, MCH, and MCHC will deviate from normal values. In this experiment, the differences in MCV, MCH, and MCHC before and after treatment were not significant, possibly because diarrhea only occurred in the calf, indicating that the combination of the veterinary acupuncture plaster and the internal compound of the present invention does not have a nutritional depletion effect on the calf. The red blood cell distribution width indexes RDWSD and RDWCV can quantitatively reflect the heterogeneity of red blood cell volume morphology. When the red blood cells are not uniform in size or self-agglutination occurs, RDWSD and RDWCV increase. In this experiment, there was no significant difference in RDWSD and RDWCV before and after Chinese medicine treatment, indicating that the combination of the veterinary acupuncture plaster and the oral compound of the present invention has no effect on the body's red blood cell morphology.

[0100] The platelet indices PLT and MPV reflect the degree of damage to the body's coagulation function and the degree of inhibition of the body's hematopoietic ability caused by pathogenic factors. The platelet distribution width indices PCT, PDW, and P-LCR reflect changes in platelet morphology. PCT is the product of PLT and MPV. When platelets are rapidly consumed, the more heterogeneous the platelet morphology, the larger the PDW. P-LCR increases when the system is likely to have mucosal bleeding or microthrombosis. Comparing the results before and after treatment with traditional Chinese medicine, there were no significant differences in MPV and PCT; there was no significant difference in platelet distribution width PDW; while PLT was significantly increased after treatment with the traditional Chinese medicine compound of the present invention (P=0.043), indicating that the traditional Chinese medicine compound of the present invention can reduce the damage of pathogenic factors to the body's coagulation function and restore the body's hematopoietic ability; the large platelet ratio P-LCR was significantly reduced after treatment (P=0.047); indicating that the combination drug treatment of the traditional Chinese veterinary medicine acupuncture plaster combined with the oral compound of the present invention can prevent the possibility of mucosal bleeding and microthrombosis in the body.

[0101] The white blood cell count (WBC) is the sum of all white blood cells in the blood. It increases when the body has acute infection, severe tissue damage, vascular hemolysis, endogenous system poisoning, etc.; it may decrease when the body has repeated infections, acute infections turn into chronic persistent infections, or viral infections reduce the body's immune capacity. Lymphocytes are mainly involved in the body's immunity. Lymphocytes can be seen to increase in viral infections and in the recovery period of acute diseases. Lymphocyte counts can be decreased in cases of persistent viral invasion, malnutrition caused by diseases, and hormone secretion disorders. Monocytes transform into phagocytes after entering tissues, inducing immunity, forming a mononuclear phagocytic system that works together with other white blood cells to exert immune functions; monocyte counts increase in the recovery period of bacterial and viral infections; granulocytes are the main type of white blood cells. Granulocyte counts (GRA) increase in cases of acute infection, blood cell destruction, and acute poisoning. In this experiment, WBC decreased after treatment, LYM increased after treatment, MON remained unchanged, and GRA decreased after treatment. This indicates that the combined drug treatment of the veterinary acupuncture plaster combined with the oral compound of the present invention can resist bacterial and viral infections and regulate the immunity of calves with diarrhea.

[0102] The oral compound medicinal ingredients for preventing and treating calf diarrhea of the present invention and the acupoint application of the traditional Chinese veterinary medicine acupuncture plaster may have a synergistic effect on inhibiting calf diarrhea pathogens and viruses.

[0103] The Chinese veterinary medicine acupuncture plaster and the internal compound combination medicine for preventing and treating calf diarrhea of the present invention may have an inhibitory effect on the pathogenic bacteria of calf diarrhea due to the synergistic effect of the alkaloids, cardiac glycosides and organic acid components contained in the compatible medicines, which mainly come from Evodia rutaecarpa, stewed costus root and wine-processed betel nut; the inhibitory effect on the pathogenic virus of calf diarrhea may be due to the synergistic effect of the triterpenes, flavonoids and glycoside-bound anthraquinone components in the prescription, which mainly come from wine-processed betel nut, wine-processed rhubarb and Evodia rutaecarpa; the "Tongqiao", "Pishu" and "Liuma" acupuncture points on the Ren and Du meridians of diarrhea calves and their collaterals in the latissimus dorsi muscles are selected, with "Pishu" as the center point, as the area where the Xinjiang green cardamom plaster is applied, and this area is the gastrointestinal transport of food and gastrointestinal rhythm of calves. The meridians that regulate movement, cardiovascular circulation, cardiopulmonary function, and immunity converge. Traditional Chinese veterinary medicine believes that the stomach and spleen are exterior and interior, the heart and small intestine are exterior and interior, and the lungs and large intestine are exterior and interior. "Tongqiao" is selected to correspond to the lungs. Stimulating it can regulate the large intestine through the exterior-interior relationship between the lungs and the large intestine, restoring its normal rhythmic movement. "Pishu" is selected to correspond to the spleen and stomach. "Pishu" is a point directly under the spleen meridian. Stimulating it can promote gastrointestinal secretion and digestion. "Liuma" is selected to correspond to the heart. Since the heart and small intestine are exterior and interior, stimulating it can improve cardiovascular circulation and regulate the rhythmic movement of the small intestine. The plaster drug Xinjiang Mung Bean is warm in nature and has the effects of opening the orifices, warming the spleen, and stopping diarrhea. The terpenes in its volatile oils are also transdermally absorbed. The antibacterial and antiviral active ingredients in the oral compound, combined with the external medication, stimulate the acupoints through application, and restore the digestive and metabolic functions of diarrheal calves, improving nutrition, maintaining normal immunity, suppressing bacteria and viruses, and restoring the health of diarrheal calves.

[0104] In the veterinary acupuncture plaster and the internal compound combination medicine of the present invention, the terpene volatile oils such as alkaloids, monoterpenes, sesquiterpenes, organic acids, triterpenes, and flavonoids contained in the internal compound cooperate with each other to resist pathogens and viruses, and serve as the main effective components; external application of Xinjiang green cardamom plaster on acupuncture points can promote the recovery of digestive and metabolic functions of calves with diarrhea, improve the body's nutrition, maintain the body's normal immunity, and play an auxiliary therapeutic role; the stimulation of acupuncture point medicine and the internal compound work together, so that the newly developed veterinary acupuncture plaster and internal compound combination for preventing and treating calf diarrhea have good effects of resisting pathogens and viruses, stopping diarrhea, strengthening the spleen and stomach, and promoting digestion, absorption and metabolism.

[0105] The veterinary acupuncture plaster and the oral compound combination medicine of the present invention have a cure rate of over 88.3% for calf diarrhea caused by multi-pathogen and multi-virus complex infection, with an effective rate of 100%; have extremely significant inhibitory effects on Proteus mirabilis and Clostridium perfringens (p=0.000); have extremely significant inhibitory effects on bovine rotavirus (BRV), bovine viral diarrhea virus (BVDV), bovine coronavirus (BCV), and bovine norovirus (BNoV) (p=0.000); and have tested twenty blood routine indicators before and after treatment. The results showed that HGB was significantly reduced after treatment compared with before treatment (P=0.041), P-LCR was significantly reduced (P=0.047), and PLT was significantly increased (P=0.043). The newly developed Chinese veterinary medicine acupuncture and oral compound combination technology for preventing and treating calf diarrhea has a significant comprehensive therapeutic effect on calf diarrhea caused by multi-virus and multi-pathogen infection. It can be used for clinical treatment of calf diarrhea caused by multi-virus and multi-pathogen complex infection, and is promoted and applied in the clinical treatment of calf diarrhea in large-scale cattle farms.

[0106] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. An acupuncture plaster and a compound medicine for internal use for preventing and treating calf diarrhea, characterized in that: The combined medicine includes a Chinese veterinary acupuncture plaster and an internal medicine compound. The components of the Chinese veterinary acupuncture plaster include an extract of Xinjiang green cardamom; the components of the internal medicine compound include stewed costus root, wine-processed betel nut, wine-processed rhubarb and Evodia rutaecarpa.

2. The combination drug according to claim 1, characterized in that The extract concentrate is obtained by extraction using an ethanol solution with a concentration of 70%-80%.

3. The combination drug according to claim 2, characterized in that The extraction weight ratio of the Xinjiang green cardamom to the ethanol solution is 1:10-20.

4. The combination drug according to claim 3, characterized in that The extract concentrate is an extract obtained by concentrating the extract of the ethanol solution.

5. The combination drug according to claim 4, characterized in that: The extract is applied to a square rubber patch and sterilized by ultraviolet light to obtain the Chinese veterinary medicine acupuncture plaster.

6. The combination drug according to claim 1, characterized in that The weight ratio of the medicinal material components of the oral compound is: 3-10 parts of stewed costus root, 6-10 parts of wine-processed betel nut, 3-10 parts of wine-processed rhubarb and 3-9 parts of evodia rutaecarpa.

7. The combination drug according to claim 6, characterized in that The weight ratio of the medicinal material components of the oral compound is: 5-7 parts of stewed costus root, 7-9 parts of wine-processed betel nut, 5-7 parts of wine-processed rhubarb and 5-7 parts of evodia rutaecarpa.

8. The combination drug according to claim 7, characterized in that: The weight ratio of the medicinal components of the oral compound is: 6.5 parts of stewed costus root, 8.5 parts of wine-processed betel nut, 6.5 parts of wine-processed rhubarb and 6.5 parts of evodia rutaecarpa.

9. The combination drug according to claim 8, characterized in that: The stewed costus root, wine-processed betel nut, wine-processed rhubarb and Evodia rutaecarpa are ground into fine powder and passed through a 60-mesh sieve.

10. A use of the combined drug according to any one of claims 1 to 9, characterized in that: The acupuncture plaster and the internal compound are simultaneously used for preventing and treating calf diarrhea.

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