Device for adjuvant therapy of osteoarthritis by using mesenchymal stem cells

Abstract

The invention discloses a device for auxiliary treatment of osteoarthritis by using mesenchymal stem cells, relates to the technical field of medical auxiliary instruments, and aims to solve the problems that an operator difficultly and conveniently observe the cell condition in real time due to the structural design of a current shaking-up device, and the cell condition cannot be conveniently observed when a culture dish is taken for observation; the technical problems that a stem cell culture solution is prone to making contact with outside air, and consequently culture fails and even pollution is caused are solved, a microscope is included, a shaking assembly located below an observation part is fixedly installed between the opposite faces of the bottom of the microscope, the shaking assembly comprises a supporting plate, and a supporting piece is fixedly installed at the top of the supporting plate; according to the invention, a base of a microscope is replaced by the swing assembly, so that an operator can directly observe the condition of the cells through the microscope when shaking up the cells in the culture dish, and the cell generation condition can be observed in real time in the shaking up process; and the cell culture effect is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical auxiliary equipment, and more particularly to a device for assisting the treatment of osteoarthritis with mesenchymal stem cells. Background Art

[0002] Osteoarthritis is a common joint disease characterized by progressive destruction of articular cartilage, joint pain, and dysfunction. Mesenchymal stem cells (MSCs) have shown promising application prospects in the treatment of osteoarthritis due to their self-renewal, multidirectional differentiation potential, and immunomodulatory properties. The research and application of MSCs in the treatment of osteoarthritis require culturing and processing the MSCs. During the culture process, an automatic shaker is typically used to shake the culture container to ensure uniform cell distribution and adequate nutrient exposure.

[0003] The current problem is that, during the process of evenly distributing cells using an automatic shaker, the shaker's structural design makes it difficult for operators to conveniently and immediately observe the cells. Furthermore, when removing the culture dish from existing culture devices for observation, the stem cell culture fluid can easily come into contact with the outside air, affecting the air environment required by the culture fluid and, in turn, the CO2 content required for stem cell growth, leading to culture failure or even contamination. Therefore, a new device is urgently needed that can both automatically shake the cells and facilitate observation of the cells during the shaking process. In light of this, we propose a device for the adjunctive treatment of osteoarthritis with mesenchymal stem cells. Summary of the Invention

[0004] The purpose of the present invention is to provide a device for the assisted treatment of osteoarthritis with mesenchymal stem cells, so as to solve the technical problem that the structural design of the current shaker makes it difficult for operators to observe the cell status in real time and conveniently, and when taking the culture dish for observation, the stem cell culture medium is easily exposed to the outside air, resulting in culture failure or even contamination.

[0005] To solve the above technical problems, the present invention provides the following technical solutions: a device for mesenchymal stem cell-assisted treatment of osteoarthritis, comprising a microscope, wherein a shaking assembly located below an observation portion is fixedly installed between opposing bottom surfaces of the microscope;

[0006] The shaking assembly includes a support plate, a support member is fixedly installed on the top of the support plate, a driving member is fixedly installed on the top of the support member, a culture dish is plugged into the top of the driving member, and a swinging assembly for controlling the up and down movement of the culture dish is rotatably installed on the outer side of the driving member, and the culture dish is movably connected to the swinging assembly.

[0007] The present invention replaces the microscope base with a rocking assembly, allowing operators to directly observe the cells through a microscope while shaking the cells in the culture dish. This enables real-time and convenient observation of cells during the shaking process, and allows timely adjustment of the shaking frequency to improve cell culture effects. This solves the problem of existing automatic shakers making it difficult to observe cells in real time, and provides more accurate data support for the research and application of mesenchymal stem cell-assisted treatment of osteoarthritis.

[0008] Preferably, the driving member includes a driving motor, a rotating rod is fixedly mounted on the output end of the driving motor, a first gear is fixedly mounted on the outer side of the rod wall of the rotating rod, and a hexagonal positioning groove is constructed at the top end of the rotating rod.

[0009] Preferably, a positioning rod movably plugged into the positioning groove is fixedly provided on the bottom of the culture dish, and an annular groove is constructed on the outer wall of the positioning rod.

[0010] Preferably, the swing assembly includes a connecting member movably mounted on the outside of the rotating rod, and the bottom sides of the bottom legs of the connecting member are rotatably mounted with second gears that mesh with the first gear, and a plurality of side edges of the second gears away from the first gear are meshed and connected with a gear ring, and a rotating seat supporting the culture dish is fixedly provided on the outer side of the gear ring, and a positioning sleeve for limiting the placement position of the culture dish is fixedly provided on the top of the rotating seat.

[0011] Preferably, the inner wall of the positioning sleeve is constructed with a corrugated ring groove that undulates up and down, and a sliding round block is slidably installed inside the corrugated ring groove. The end of the sliding round block is telescopically installed with a locking block that is engaged with the ring groove, and the upper and lower side edges of the locking block are both arc-shaped.

[0012] Preferably, a plurality of blocking pieces cooperating with the blocking block are arranged in an array on the inner wall of the annular groove, and a side surface of the blocking piece is provided with an inclined surface for squeezing the blocking block to extend and retract.

[0013] Preferably, the support member includes two fixed blocks fixedly arranged on the top of the support plate, and an arc-shaped support plate connected to the drive motor is rotatably installed between the two fixed blocks. Two support springs are fixedly provided on the outer side surfaces at both ends of the arc-shaped support plate, and the other end of each support spring is fixedly provided with a triangular block fixedly connected to the top of the support plate.

[0014] Preferably, an extension plate is fixedly provided on the rod wall of the rotating rod, and a gravity block is integrally formed on the bottom side surface of the end of the extension plate.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. By replacing the microscope base with a rocking assembly, the present invention allows the operator to observe the cells directly through the microscope while shaking the cells in the culture dish. This enables real-time and convenient observation of cells during the shaking process, and the shaking frequency can be adjusted in a timely manner to improve the cell culture effect. This solves the problem of existing automatic shakers that make it difficult to observe cells in real time, and provides more accurate data support for the research and application of mesenchymal stem cell-assisted treatment of osteoarthritis.

[0017] 2. The present invention drives the rotating rod to rotate by a driving motor to control the rotation of the culture dish plugged therein. The rotation makes the cells in the culture dish more evenly dispersed in the culture medium under the action of centrifugal force, avoiding the cells from gathering in a local area, which is conducive to the cells fully contacting the nutrients and oxygen in the culture medium, providing a good environment for cell growth, and improving the quality and efficiency of cell culture. The design of the swing component can make the culture dish move up and down during the rotation process. This structural coordination increases the functionality of the shaking operation, which is beneficial to the cultivation of cells. The positioning sleeve in the swing component cooperates with the annular groove of the culture dish through the corrugated ring groove, the sliding circle and the locking block. When the culture dish is realized to fluctuate up and down, its motion trajectory and amplitude can be better controlled, the consistency of the upper and lower amplitudes can be maintained, and the uniformity of the cell shaking can be further ensured.

[0018] 3. The present invention can also achieve a reverse swinging effect through the cooperation of the arc-shaped support plate and the support spring in the support member. The low-speed rotation of the rotating rod can move the gravity block at the bottom of the extension plate to the direction of the support spring. Increasing the weight on this side can squeeze the support spring on the same side to contract, completing the downward movement effect. In this way, the downward movement direction is repeatedly changed to achieve a swinging effect, further increasing the functionality of the shaking operation. In addition, the consistency of weight can maintain the same flip angle, ensuring the uniformity of the shaking operation and enhancing the cell culture effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2 Schematic diagram of the structure of the shaking assembly of the present invention;

[0021] Figure 3 It is a schematic cross-sectional view of a local structure of the present invention;

[0022] Figure 4 for Figure 2 Schematic diagram of the local structure;

[0023] Figure 5 for Figure 4 Schematic diagram of the cross-section structure;

[0024] Figure 6 for Figure 5Schematic diagram of the partial structure cut away;

[0025] Figure 7 Schematic diagram of the structure of the culture dish in the present invention.

[0026] Description of the numbers in the figure:

[0027] 1. Microscope; 2. Shaking assembly; 3. Support plate; 4. Support member; 401. Fixed block; 402. Arc support plate; 403. Support spring; 404. Triangular block; 5. Driving member; 501. Driving motor; 502. Rotating rod; 503. First gear; 504. Positioning groove; 505. Extension plate; 506. Gravity block; 6. Culture dish; 601. Positioning rod; 602. Annular groove; 603. Shifting plate; 7. Swinging assembly; 701. Coupling member; 702. Second gear; 703. Gear ring; 704. Rotating seat; 705. Positioning sleeve; 706. Waveform ring groove; 707. Sliding round block; 708. Positioning block. DETAILED DESCRIPTION

[0028] like Figures 1 to 7 As shown, the present invention relates to a device for mesenchymal stem cell-assisted treatment of osteoarthritis, comprising a microscope 1, with a shaking assembly 2 located below an observation portion fixedly installed between opposing surfaces of the bottom of the microscope 1; through the combination of the microscope 1 and the shaking assembly 2, the cell generation status can be observed at all times during the cell cultivation process, and the shaking frequency can be easily adjusted during the observation process, providing accurate data support for subsequent treatment research.

[0029] The shaking assembly 2 includes a support plate 3, a support member 4 is fixedly installed on the top of the support plate 3, a driving member 5 is fixedly installed on the top of the support member 4, a culture dish 6 is plugged into the top of the driving member 5, and a swinging assembly 7 for controlling the up and down movement of the culture dish 6 is rotatably installed on the outer side of the driving member 5, and the culture dish 6 is movably connected to the swinging assembly 7; the support of the support plate 3 can limit the installation position of the support member 4 and the driving member 5, so that the culture dish 6 can be aligned with the observation window position of the microscope 1, and the cover of the culture dish 6 is made of transparent material, so that the internal cell generation situation can be observed through the cover. Under the operation of the driving member 5, the rotation of the culture dish 6 can be controlled. This mechanical drive method can provide stable and continuous power. Compared with manual hand shaking, the intensity, frequency and time of shaking can be accurately controlled to ensure the consistency of each shaking, so that the cells are evenly distributed in the culture dish 6. With the setting of the swinging assembly 7, the culture dish 6 can move up and down during rotation, further improving the effect of cell culture. In this way, the rotation frequency of the culture dish 6 can be adjusted under observation through the microscope 1 to achieve a suitable shaking speed.

[0030] In an embodiment of the present invention, the driving member 5 includes a driving motor 501, a rotating rod 502 is fixedly installed at the output end of the driving motor 501, a first gear 503 is fixedly installed on the outer side of the rod wall of the rotating rod 502, a hexagonal positioning groove 504 is constructed at the top of the rotating rod 502, and a positioning rod 601 movably plugged into the positioning groove 504 is fixedly provided at the bottom of the culture dish 6, and an annular groove 602 is constructed on the outer wall of the positioning rod 601; the installation position of the culture dish 6 can be positioned by plugging the positioning rod 601 into the positioning groove 504, so that the culture dish 6 can rotate synchronously with the rotating rod 502 to achieve a self-rotation effect, which makes it easy to take and place the culture dish 6.

[0031] In an embodiment of the present invention, the swing assembly 7 includes a coupling 701 movably mounted on the outside of the rotating rod 502, and the bottom sides of the bottom legs of the coupling 701 are rotatably mounted with second gears 702 that mesh with the first gear 503, and a plurality of second gears 702 are meshed and connected with the side edges away from the first gear 503 with a gear ring 703, and the outer side of the gear ring 703 is fixedly provided with a rotating seat 704 that supports the culture dish 6, and the top of the rotating seat 704 is fixedly provided with a positioning sleeve 705 for limiting the placement position of the culture dish 6; through the effect of the combination of the first gear 503 and the outer teeth of the second gear 702, the second gear 702 can be made to rotate in the opposite direction, so that the inner teeth of the gear ring 703 meshing with it can achieve the same direction rotation effect, causing the rotating seat 704 and the rotating rod 502 to be in the opposite direction of movement.

[0032] When the cam 708 is in the closed position, the stopper 703 is pressed against the stopper 706 to stop the cam 708 from moving upwards and downwards, thereby preventing the cam 708 from moving downwards.

[0033] In an embodiment of the present invention, the support member 4 includes two fixed blocks 401 fixedly arranged on the top of the support plate 3, an arc-shaped support plate 402 connected to the drive motor 501 is rotatably installed between the two fixed blocks 401, two support springs 403 are fixedly provided on the outer side surfaces of both ends of the arc-shaped support plate 402, and the other end of each support spring 403 is fixedly provided with a triangular block 404 fixedly connected to the top of the support plate 3, an extension plate 505 is also fixedly provided on the rod wall of the rotating rod 502, and a gravity block 506 is integrally formed on the bottom side surface of the end of the extension plate 505; by rotating the arc The shaped support plate 402 can maintain a stable state with the support spring 403 supporting it under the condition of balanced weight. When the rotating rod 502 drives the gravity block 506 to move to one side of the support spring 403, the weight on this side can be increased, thereby compressing the support spring 403 on this side to contract, causing the culture dish 6 to tilt. When the gravity block 506 moves to a position flush with the fixed block 401, the stable state can be restored. In this way, the culture dish 6 can achieve a swinging effect under repeated movement, and the swinging angle can be kept uniform due to the fixed weight, further enhancing the cell culture effect.

[0034] Working Principle: This embodiment provides a device for the assisted treatment of osteoarthritis with mesenchymal stem cells. During use, the culture dish 6 containing the cells is first placed on the inner side of the top of the positioning sleeve 705 and pressed downward to allow it to enter the interior. Simultaneously, the retaining blocks 708 on both sides are squeezed to move toward the inside of the sliding circular block 707. The positioning rod 601 at the bottom of the culture dish 6 can be inserted into the positioning groove 504 at the top of the rotating rod 502. When the position is in place, the retaining blocks 708 can be snapped into the annular groove 602. Thus, when the driving member 5 is in operation, the drive motor 501 in the driving member 5 serves as the power source, and its output end drives the rotating rod 502 to rotate. Because the first gear 503 is fixedly mounted on the outer side of the rod wall of the rotating rod 502, the first gear 503 rotates with the rotating rod 502. Meanwhile, the top of the rotating rod 502 is constructed with a hexagonal positioning slot 504, which provides a positioning interface for the installation of the culture dish 6. A positioning rod 601, fixed to the bottom of the culture dish 6, is shaped to match the positioning slot 504 at the top of the rotating rod 502 and can be flexibly inserted into the positioning slot 504, achieving the initial positioning and installation of the culture dish 6 on the drive member 5. Furthermore, the outer wall of the positioning rod 601 is constructed with an annular groove 602, which is a key structure for interacting with the swing assembly 7 to achieve the up and down movement of the culture dish 6. In the swing assembly 7, a coupling 701 is movably mounted on the outer side of the rotating rod 502. The bottom side of the coupling leg is rotatably mounted with a second gear 702 that meshes with the first gear 503. When the first gear 503 rotates with the rotating rod 502, it meshes with the second gear 702, driving the second gear 702 to rotate. The sides of the second gears 702 away from the first gear 503 are meshed with a gear ring 703, so the rotation of the second gears 702 will further drive the rotation of the gear ring 703. The cooperation between the external teeth and the internal teeth allows the gear ring 703 to achieve the effect of rotating in the opposite direction to the first gear 503. The outer side of the gear ring 703 is fixedly provided with a rotating seat 704 that supports the culture dish 6. Therefore, the rotating seat 704 will drive the positioning sleeve 705 to rotate in the opposite direction. The inner wall of the positioning sleeve 705 is constructed with an undulating wave-shaped annular groove 706, and a sliding round block 707 is slidably installed inside. The end of the sliding round block 707 is telescopically installed with a blocking block 708 that engages with the annular groove 602, and the upper and lower sides of the blocking block 708 are both arc-shaped. When the culture dish 6 rotates with the rotating seat 704, the locking block 708 will slide in the annular groove 602. When the locking block 708 slides in the annular groove 602 and contacts the blocking piece 603, due to the inclined design of the blocking piece 603, the locking block 708 will be squeezed and expanded, so that it can pass through the blocking piece 603 smoothly.At the same time, due to the ups and downs of the wave-shaped annular groove 706 and its reverse movement with the culture dish 6, the stopper 603 will be squeezed to one side of the plane of the stopper 603, achieving the role of limiting, which will cause the sliding round block 707 to drive the blocking block 708 to move up and down in the annular groove 602, thereby realizing the up and down fluctuation of the culture dish 6 during the rotation process, simulating a more complex dynamic culture environment, and during the rotation of the rotating rod 502, the gravity block 506 on the extension plate 505 will be driven to rotate synchronously, so that under low-speed rotation, the gravity block 506 can be displaced to one side of the support spring 403, so that the weight on this side increases, thereby pressing the support spring 403 on this side to contract downward, causing the arc-shaped support plate 402 to tilt. 06 can be reset when it moves to a position parallel to the fixed block 401, and the repeated movement can achieve a swinging effect, thereby further increasing the swinging direction of the culture dish 6 and improving the cell cultivation effect. In addition, such a structural combination can change the swinging frequency according to the rotation speed of the rotating rod 502, which can effectively simulate the dynamic environment in the human joint and provide a physical stimulation closer to the real situation in the body for the growth and differentiation of mesenchymal stem cells. The shaking component 2 is set below the observation part of the microscope 1, which is convenient for researchers to observe and record the growth status of cells at any time through the microscope 1 during the process of cultivating mesenchymal stem cells, and timely grasp the changes in cells, provide accurate data support for subsequent treatment research, and improve cell cultivation effects.

[0035] The embodiments disclosed in the present invention are preferred embodiments, but are not limited to them. Ordinary technicians in this field can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. As long as they do not deviate from the spirit of the present invention, they are all within the scope of protection of the present invention.

Claims

1. A device for mesenchymal stem cell-assisted treatment of osteoarthritis, characterized in that: The invention comprises a microscope (1), wherein a shaking assembly (2) located below an observation portion is fixedly mounted between facing surfaces of the bottom of the microscope (1); The shaking assembly (2) comprises a supporting plate (3), a supporting member (4) is fixedly mounted on the top of the supporting plate (3), a driving member (5) is fixedly mounted on the top of the supporting member (4), a culture dish (6) is plugged and mounted on the top of the driving member (5), a swinging assembly (7) for controlling the up and down movement of the culture dish (6) is rotatably mounted on the outer side of the driving member (5), and the culture dish (6) is movably connected to the swinging assembly (7) by snap-fitting.

2. The device for mesenchymal stem cell-assisted treatment of osteoarthritis according to claim 1, characterized in that: The driving member (5) comprises a driving motor (501), a rotating rod (502) is fixedly mounted on the output end of the driving motor (501), a first gear (503) is fixedly mounted on the outer side of the rod wall of the rotating rod (502), and a hexagonal positioning groove (504) is constructed at the top end of the rotating rod (502).

3. The device for mesenchymal stem cell-assisted treatment of osteoarthritis according to claim 2, characterized in that: A positioning rod (601) movably plugged into the positioning groove (504) is fixedly provided at the bottom of the culture dish (6), and an annular groove (602) is constructed on the outer wall of the positioning rod (601).

4. The device for mesenchymal stem cell-assisted treatment of osteoarthritis according to claim 3, characterized in that: The swing assembly (7) comprises a coupling (701) movably sleeved on the outside of a rotating rod (502); a second gear (702) meshing with a first gear (503) is rotatably mounted on the bottom side of the bottom legs of the coupling (701); a plurality of second gears (702) away from the first gear (503) are meshed and connected with a gear ring (703); a rotating seat (704) for supporting a culture dish (6) is fixedly provided on the outer side of the gear ring (703); and a positioning sleeve (705) for limiting the placement position of the culture dish (6) is fixedly provided on the top of the rotating seat (704).

5. The device for mesenchymal stem cell-assisted treatment of osteoarthritis according to claim 4, characterized in that: The inner wall of the positioning sleeve (705) is constructed with a wave-shaped annular groove (706) that undulates up and down. A sliding round block (707) is slidably installed inside the wave-shaped annular groove (706). The end of the sliding round block (707) is telescopically installed with a locking block (708) that is engaged with the annular groove (602), and the upper and lower side edges of the locking block (708) are both arc-shaped.

6. The device for mesenchymal stem cell-assisted treatment of osteoarthritis according to claim 5, characterized in that: A plurality of stop plates (603) cooperating with the stop block (708) are arranged in an array on the inner wall of the annular groove (602), and a side surface of the stop plate (603) is provided with an inclined surface for facilitating the expansion and contraction of the stop block (708).

7. The device for mesenchymal stem cell-assisted treatment of osteoarthritis according to claim 1, characterized in that: The support member (4) comprises two fixed blocks (401) fixedly arranged on the top of the support plate (3); an arc-shaped support plate (402) connected to the drive motor (501) is rotatably mounted between the two fixed blocks (401); two support springs (403) are fixedly arranged on the outer side surfaces of both ends of the arc-shaped support plate (402); and a triangular block (404) fixedly connected to the top of the support plate (3) is fixedly arranged on the other end of each support spring (403).

8. The device for mesenchymal stem cell-assisted treatment of osteoarthritis according to claim 2, characterized in that: An extension plate (505) is also fixedly provided on the rod wall of the rotating rod (502), and a gravity block (506) is integrally formed on the bottom side surface of the end of the extension plate (505).

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