Earthworm compound protein powder and preparation method thereof

Abstract

The invention discloses earthworm compound protein powder and a preparation method thereof. The earthworm compound protein powder comprises the following raw materials in parts by weight: 15-25 parts of earthworm polypeptide powder, 10-20 parts of oyster peptide, 5-10 parts of selenium-enriched giant salamander protein powder, 8-15 parts of modified peanut protein, 0.5-1.2 parts of selenomethionine and 3-5 parts of a compound mineral carrier. Advantages of animal protein and plant protein are fused, and the selenium-rich protein has the characteristics of high bioavailability and selenium enrichment.

Description

Technical Field

[0001] The invention relates to the technical field of compound protein powder preparation, and in particular to earthworm compound protein powder and a preparation method thereof. Background Art

[0002] Earthworms, commonly known as earthworms, are a high-quality animal protein resource and a commonly used traditional Chinese medicine. They possess dual anticoagulant and thrombolytic properties, antihypertensive and immune-enhancing properties, as well as bidirectional spermaticide and sperm-strengthening effects. They also possess anticancer, antipyretic, anti-cerebral ischemia, anti-asthmatic, antibacterial, antioxidant, and wound repair and cell regeneration properties. Furthermore, peptide extracts obtained through enzymatic hydrolysis of earthworms exhibit antioxidant, immune-enhancing, and antibacterial properties. Earthworm protein contains various enzymes and trace elements, including a small amount of selenium. Clinical trials have shown that earthworm protein can soften blood vessels, improve vascular blockage, enhance the body's natural anticoagulant function, and activate the differentiation properties of endothelial cells.

[0003] Selenium is an essential trace nutrient for the human body, boasting numerous benefits, including anti-cancer, immunity-boosting, and anti-aging. It's known as the "fire of life" and the "king of cancer prevention." Approximately two-thirds of my country's population, nearly 700 million people, suffer from selenium deficiency. Improving immunity through dietary selenium and promoting health and longevity are key livelihood issues globally. Therefore, the development of selenium-rich earthworm protein powder is of great significance.

[0004] The existing compound protein powder has the following problems: 1. The amino acid composition of a single protein source is unbalanced; 2. The biological conversion rate of selenium in the selenium-enriched process is low; 3. The enzymatic hydrolysis process is not optimized for the characteristics of complex proteins. Summary of the Invention

[0005] The problem to be solved by the present invention is to provide an earthworm composite protein powder and a preparation method thereof, which combines the advantages of animal protein and plant protein and has high bioavailability and selenium-rich properties.

[0006] The present invention provides a technical solution to solve the above problems: a ground dragon compound protein powder, which comprises, by weight, 15 to 25 parts of earthworm polypeptide powder, 10 to 20 parts of oyster peptide, 5 to 10 parts of selenium-enriched giant salamander protein powder, 8 to 15 parts of modified peanut protein, 0.5 to 1.2 parts of selenomethionine, and 3 to 5 parts of a composite mineral carrier.

[0007] Preferably, the earthworm composite protein powder comprises, by weight, 15 parts of earthworm polypeptide powder, 15 parts of oyster peptide, 6 parts of selenium-enriched giant salamander protein powder, 8 parts of modified peanut protein, 0.8 parts of selenomethionine, and 3 parts of composite mineral carrier.

[0008] Preferably, the earthworm composite protein powder comprises, by weight, 20 parts of earthworm polypeptide powder, 10 parts of oyster peptide, 8 parts of selenium-enriched giant salamander protein powder, 10 parts of modified peanut protein, 0.5 parts of selenomethionine, and 4 parts of composite mineral carrier.

[0009] Preferably, the earthworm composite protein powder comprises, by weight, 25 parts of earthworm polypeptide powder, 12 parts of oyster peptide, 10 parts of selenium-enriched giant salamander protein powder, 15 parts of modified peanut protein, 1.2 parts of selenomethionine, and 5 parts of composite mineral carrier.

[0010] Preferably, the degree of hydrolysis of the earthworm polypeptide powder is 8-12%.

[0011] Preferably, the molecular weight of the oyster peptide is 500-1000 Da.

[0012] Preferably, the composite mineral carrier comprises zinc gluconate and sodium selenite.

[0013] The present invention also discloses a method for preparing the earthworm composite protein powder as described in any one of the above, the preparation method comprising the following steps: (1) Preparation of earthworm polypeptides: a. Cultivate fresh earthworms in a selenium-enriched medium containing 0.1% sodium selenite for 7 days, then drain the sand in clean water and soak in 0.025% saline. b. Use a stepwise enzymatic hydrolysis method: first add subtilisin and react at 50°C, pH 7.5 for 1 hour, then add flavor protease and react at 55°C, pH 6.5 for 0.5 hour; c. Add 1.2 times the weight of earthworms to the enzymatic hydrolysate to perform the Maillard reaction; (2) Preparation of oyster peptide-peanut protein complex: a. Peanut protein powder and oyster peptide were mixed at a ratio of 1:2, 0.8% pullulan was added, and a composite carrier was formed by Maillard reaction; b loaded composite minerals, composite minerals zinc: selenium molar ratio of 1:1.2; (3) Selenium-enriched treatment of giant salamander protein: The pulsed electric field is used to assist in selenium enrichment, increasing the selenium content by 40%; (4) Preparation of final product: The components are mixed with microencapsulated selenomethionine, and then purified in a purification device to remove larger particle impurities, and finally granulated using a fluidized bed.

[0014] Preferably, the field intensity of the pulse electric field in (3) is 20 kV / cm, and the pulse width is 50 μs.

[0015] Preferably, the purification device in (4) includes a purification barrel and a plurality of filter components, wherein a filter chamber and a liquid collecting chamber are provided in the purification barrel, wherein the liquid collecting chamber is provided at the lower end of the filter chamber, wherein a plurality of filter cavities are provided in the filter chamber, and wherein the plurality of filter components are detachably installed in the filter chamber.

[0016] Compared with the prior art, the advantages of the present invention are: (1) Synergistic effect: earthworm peptide (containing hydroxyproline) and oyster peptide (rich in taurine) form a complementary amino acid spectrum, with a PDCAAS value of 0.98; (2) Efficient utilization of selenium: Through the triple selenium enrichment pathway of biological transformation (earthworms) + physical enhancement (pulsed electric field) + chemical chelation, the bioavailability of selenium is increased to 85.7%; (3) Flavor improvement: Maillard reaction products mask the fishy smell and reduce the bitterness value; (4) Stability innovation: The pullulan-protein composite carrier extends the storage stability of minerals at room temperature to 18 months. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings described herein are used to provide further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0018] Figure 1 It is a flowchart of the preparation method of the earth dragon compound protein powder of the present invention; Figure 2 It is a schematic diagram of the three-dimensional structure of the purification device of the present invention; Figure 3 is a cross-sectional view of a purification device of the present invention; Figure 4 yes Figure 3 A magnified schematic diagram of point A in the middle; Figure 5 yes Figure 3 A magnified schematic diagram of point B in the middle; Figure 6 It is a partial structural schematic diagram of the positioning release assembly of the purification device of the present invention; Figure 7 is a bottom view of the positioning release assembly of the purification device of the present invention; Figure 8 It is a partial structural schematic diagram of the installation pipe of the purification device of the present invention.

[0019] The attached drawings are marked with: 1. purification barrel, 2. mounting hole one, 3. filter chamber, 4. liquid inlet hole, 5. mounting tube, 6. hollow fiber filter tube, 7. switch baffle, 8. liquid collecting chamber, 9. filter chamber, 10. drive ring, 11. annular groove, 12. spring three, 13. positioning hole three, 14. movable rod, 15. positioning column one, 16. mounting groove one, 17. spring one, 18. matching groove one, 19. drive block one, 20. sealing ring, 21. mounting seat, 22. spring four, 23. positioning column three, 24. magnet block, 25. positioning column two, 26. spring two, 27. drive block two, 28. matching groove two, 29. positioning hole one, 30. docking tube, 31. docking chamber. DETAILED DESCRIPTION

[0020] The following will describe the embodiments of the present invention in detail with reference to the accompanying drawings and examples, so that the implementation process of how the present invention applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0021] In the description of the present invention, it should be noted that, for directional words, such as the terms "center", "horizontal", "longitudinal", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and so on, indicating directions and positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and cannot be understood as limiting the specific scope of protection of the present invention.

[0022] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features. Therefore, the terms "first" and "second" may explicitly or implicitly refer to one or more of these features. Throughout the description of the present invention, "several" means two or more, unless otherwise specifically defined.

[0023] In the present invention, unless otherwise specified or limited, the terms "assemble," "connect," and "connect" should be understood in a broad sense. For example, they may refer to fixed connection, detachable connection, or integral connection; mechanical connection; direct connection, connection through an intermediate medium, or internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0024] It will be understood that when used in this specification and the appended claims, the terms “comprises” and “comprising” indicate the presence of described features, integers, steps, operations, elements and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or groups thereof.

[0025] It should also be understood that the terms used in this description of the embodiments of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the embodiments of the present invention. As used in the description of the embodiments of the present invention and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms unless the context clearly indicates otherwise. Example 1

[0026] This embodiment discloses a groundworm composite protein powder, which comprises, by weight, 15 to 25 parts of earthworm polypeptide powder, 10 to 20 parts of oyster peptide, 5 to 10 parts of selenium-enriched giant salamander protein powder, 8 to 15 parts of modified peanut protein, 0.5 to 1.2 parts of selenomethionine, and 3 to 5 parts of a composite mineral carrier; wherein the degree of hydrolysis of the earthworm polypeptide powder is 8 to 12%; the molecular weight of the oyster peptide is 500 to 1000 Da; and the composite mineral carrier comprises zinc gluconate and sodium selenite. Example 2

[0027] This embodiment discloses a ground dragon compound protein powder, which comprises, by weight, 15 parts of earthworm polypeptide powder, 15 parts of oyster peptide, 6 parts of selenium-enriched giant salamander protein powder, 8 parts of modified peanut protein, 0.8 parts of selenomethionine, and 3 parts of a composite mineral carrier. Example 3

[0028] This embodiment discloses a ground dragon compound protein powder, which comprises, by weight, 20 parts of earthworm polypeptide powder, 10 parts of oyster peptide, 8 parts of selenium-enriched giant salamander protein powder, 10 parts of modified peanut protein, 0.5 parts of selenomethionine, and 4 parts of a composite mineral carrier. Example 4

[0029] This embodiment discloses a ground dragon compound protein powder, which comprises, by weight, 25 parts of earthworm polypeptide powder, 12 parts of oyster peptide, 10 parts of selenium-enriched giant salamander protein powder, 15 parts of modified peanut protein, 1.2 parts of selenomethionine, and 5 parts of a composite mineral carrier. Example 5

[0030] This embodiment discloses a method for preparing earthworm compound protein powder, which is applied in Examples 1-4. The preparation method comprises the following steps: (1) Preparation of earthworm polypeptides: a. Cultivate fresh earthworms in a selenium-enriched medium containing 0.1% sodium selenite for 7 days, then drain the sand in clean water and soak in 0.025% saline. b. Use a stepwise enzymatic hydrolysis method: first add subtilisin and react at 50°C, pH 7.5 for 1 hour, then add flavor protease and react at 55°C, pH 6.5 for 0.5 hour; c. Add 1.2 times the weight of earthworms to the enzymatic hydrolysate to perform the Maillard reaction; (2) Preparation of oyster peptide-peanut protein complex: a. Peanut protein powder and oyster peptide were mixed at a ratio of 1:2, 0.8% pullulan was added, and a composite carrier was formed by Maillard reaction; b loaded composite minerals, composite minerals zinc: selenium molar ratio of 1:1.2; (3) Selenium-enriched treatment of giant salamander protein: The pulsed electric field is used to assist in selenium enrichment, increasing the selenium content by 40%; (4) Preparation of final product: The components are mixed with microencapsulated selenomethionine, and then purified in a purification device to remove larger particle impurities, and finally granulated using a fluidized bed.

[0031] The field strength of the pulse electric field in (3) is 20 kV / cm, and the pulse width is 50 μs. Example 6

[0032] like Figure 2-Figure 8 As shown, this embodiment discloses a purification device, which is applied in Example 5. Specifically, the purification device includes a purification barrel 1 and several filter components. The purification barrel 1 is provided with a filter chamber 9 and a liquid collection chamber 8. The liquid collection chamber 8 is provided at the lower end of the filter chamber 9. The filter chamber 9 is provided with several filter cavities 3. Several of the filter components are detachably installed in the filter chamber 3.

[0033] The upper cover of the purification barrel 1 is provided with a plurality of mounting holes 2 for cooperating with the filter assembly, and the lower end of the filter chamber 9 is provided with a mounting hole 2 for cooperating with the filter assembly.

[0034] The filter assembly includes a mounting tube 5, a hollow fiber filter tube 6, a positioning assembly 1, a positioning assembly 2 and a positioning release assembly. The hollow fiber filter tube 6 is coaxially arranged inside the mounting tube 5. The positioning assembly 1 is arranged at the mounting hole 1 2 for positioning the upper part of the mounting tube 5. The positioning assembly 2 is arranged at the mounting hole 2 for positioning the lower part of the mounting tube 5. The positioning release assembly is arranged on the mounting tube 5 for releasing the positioning of the positioning assembly 1 and the positioning assembly 2.

[0035] Among them, a plurality of liquid inlet holes 4 are provided on the mounting tube 5, the positioning component 1 includes a spring 17 and a positioning column 15, and a mounting groove 16 is provided on the hole wall of the mounting hole 2, one end of the spring 17 is fixedly connected to the bottom of the mounting groove 16, and the other end is fixedly connected to the positioning column 15, the positioning column 15 extends out of the mounting groove 16 away from the end of the spring 17, and a positioning hole 29 is provided on the mounting tube 5 at a position corresponding to the mounting groove 16; the mounting component 2 includes a spring 26 and a positioning column 25, and a mounting groove 2 is provided on the hole wall of the mounting hole 2, one end of the spring 26 is fixedly connected to the bottom of the mounting groove 2, and the other end is fixedly connected to the positioning column 25, the positioning column 25 extends out of the mounting groove 2 away from the end of the spring 26, and a positioning hole 2 is provided on the mounting tube 5 at a position corresponding to the mounting groove 2; The positioning release assembly includes a drive ring 10, a plurality of movable rods 14 and a plurality of spring threes 12. The plurality of movable rods 14 are distributed in an annular array at the lower end of the drive ring 10. The upper end of the mounting tube 5 is provided with an annular groove 11 that cooperates with the drive ring 10. The spring three 12 is arranged in the annular groove 11 and one end is fixedly connected to the bottom of the annular groove 11, and the other end is fixedly connected to the bottom of the drive ring 10. A movable hole that cooperates with the movable rod 14 is provided in the mounting tube 5. The upper and lower ends of the movable rod 14 are respectively provided with a drive block 19 and a drive block 27. A matching groove 18 that cooperates with the drive block 19 and a matching groove 12 that cooperates with the drive block 19 are provided in the mounting tube 5. The driving block 27 cooperates with the matching groove 28, and the matching groove 18 is connected with the positioning hole 1 29, and the matching groove 28 is connected with the positioning hole 2. When the driving ring 10 is pressed downward, the driving ring 10 drives the movable rod 14 to move downward, and then drives the driving block 19 and the driving block 2 27 to move downward. In the process of the driving block 19 moving downward, the positioning column 15 is squeezed so that the positioning column 15 retracts into the installation groove 1 16. In the process of the driving block 2 27 moving downward, the positioning column 2 25 is squeezed so that the positioning column 2 25 retracts into the installation groove 2, thereby releasing the positioning component 1 and the positioning component 2 from the positioning of the mounting tube 5.

[0036] A docking cavity 31 is provided at the upper end of the mounting tube 5, and a docking tube 30 which is adapted to the shape of the docking cavity 31 is provided at the lower end of the mounting tube 5. A plurality of positioning holes three 13 are provided on the cavity wall of the docking cavity 31, and a plurality of mounting seats 21 are provided on the docking tube 30. A mounting groove three is provided in the mounting seat 21, and a spring four 22 and a positioning column three 23 are provided in the mounting groove three. One end of the spring four 22 is fixedly connected to the groove bottom of the mounting groove, and the other end is fixedly connected to the positioning column three 23. When the two filter components are docked up and down, the positioning column of the docking tube 30 of the upper filter component is inserted into the positioning hole three 13 in the docking cavity 31 of the lower filter component, and the positioning column three 23 is made of magnets. A plurality of magnet blocks 24 are provided at the mounting hole two, and the magnet blocks 24 are arranged to repel the positioning column three 23, and a sealing ring 20 is provided in both the mounting hole one 2 and the mounting hole two.

[0037] In the above scheme, when replacing the filter assembly, align the docking tube at the lower end of the new filter assembly with the filter assembly to be replaced, and insert the docking tube into the docking cavity of the old filter assembly. During the insertion process, the new filter assembly drives the driving ring to press downward, and the movable rod follows the driving ring to press downward, and then the driving block 1 and the driving block 2 on the movable rod both move downward. During the downward movement of the driving block 1, the positioning post 1 is squeezed to make the positioning post 1 retract into the inside of the installation groove 1. During the downward movement of the driving block 2, the positioning post 2 is squeezed to make the positioning post 2 retract into the inside of the installation groove 2. The positioning of the mounting tube 5 by the positioning assembly 1 and the positioning assembly 2 is released. At the same time, the positioning post of the docking tube 30 of the new filter assembly is inserted into the positioning hole 3 13 in the docking cavity 31 of the old filter assembly, and the new filter assembly is aligned with the old filter assembly. Then position it, and then continue to press the new filter assembly downward until the position of the positioning post three corresponds to the position of the magnet block in the mounting hole two. At this time, the magnet block generates a repulsive force on the positioning post three to press the positioning post back into the mounting groove three, and the old filter assembly falls into the liquid collection chamber under the action of its own gravity. At the same time, the positioning post one in the positioning assembly one is inserted into the positioning hole one on the mounting tube of the new filter assembly, and the positioning post two in the positioning assembly two is inserted into the positioning hole two on the mounting tube of the new filter assembly, and the positioning assembly is installed. Through the above scheme, when the old filter assembly needs to be replaced, the filter assembly can be quickly replaced through the above structure, and there is no need to disassemble the purification barrel for replacement, which improves the replacement efficiency. At the same time, during the replacement process, the purification and filtration work can continue, effectively ensuring the filtration efficiency.

[0038] It should be noted that an openable and closable switch baffle 7 is provided in the liquid collecting chamber, and the installation tube 5 dropped into the liquid collecting chamber can be taken out by opening the switch baffle 7 .

[0039] In this embodiment, it should be noted that the purification barrel of this device is made of transparent material. When the filtering effect of the filter component in a certain filter cavity is reduced, its filtering capacity is reduced, and the liquid passing through the hollow fiber filter tube becomes less. Therefore, the staff can observe through the transparent purification barrel that the liquid level in the filter cavity is significantly higher than the liquid levels of the other filter cavities, and can replace the filter component in the corresponding filter cavity.

[0040] The above description is merely a description of the preferred embodiment of the present invention and is not to be construed as limiting the scope of the claims. The present invention is not limited to the above embodiment, and variations in the specific structure are permitted. Any variations within the scope of the independent claims of the present invention are also within the scope of protection of the present invention.

Claims

1. A ground dragon compound protein powder, characterized in that: The raw materials of the earthworm composite protein powder include, by weight, 15 to 25 parts of earthworm polypeptide powder, 10 to 20 parts of oyster peptide, 5 to 10 parts of selenium-enriched giant salamander protein powder, 8 to 15 parts of modified peanut protein, 0.5 to 1.2 parts of selenomethionine, and 3 to 5 parts of composite mineral carrier.

2. A kind of earthworm composite protein powder according to claim 1, it is characterized in that: The earthworm composite protein powder comprises, by weight, 15 parts of earthworm polypeptide powder, 15 parts of oyster peptide, 6 parts of selenium-enriched giant salamander protein powder, 8 parts of modified peanut protein, 0.8 parts of selenomethionine, and 3 parts of composite mineral carrier.

3. A kind of earthworm composite protein powder according to claim 1, it is characterized in that: The earthworm composite protein powder comprises, by weight, 20 parts of earthworm polypeptide powder, 10 parts of oyster peptide, 8 parts of selenium-enriched giant salamander protein powder, 10 parts of modified peanut protein, 0.5 parts of selenomethionine, and 4 parts of composite mineral carrier.

4. A kind of earthworm composite protein powder according to claim 1, it is characterized in that: The earthworm composite protein powder comprises, by weight, 25 parts of earthworm polypeptide powder, 12 parts of oyster peptide, 10 parts of selenium-enriched giant salamander protein powder, 15 parts of modified peanut protein, 1.2 parts of selenomethionine, and 5 parts of composite mineral carrier.

5. A kind of earthworm composite protein powder according to claim 1, it is characterized in that: The hydrolysis degree of the earthworm polypeptide powder is 8-12%.

6. A kind of earthworm composite protein powder according to claim 1, it is characterized in that: The molecular weight of the oyster peptide is 500-1000 Da.

7. A kind of earthworm composite protein powder according to claim 1, it is characterized in that: The composite mineral carrier comprises zinc gluconate and sodium selenite.

8. A method for preparing the earthworm composite protein powder according to any one of claims 1 to 7, characterized in that: The preparation method comprises the following steps: (1) Preparation of earthworm polypeptides: a. Cultivate fresh earthworms in a selenium-enriched medium containing 0.1% sodium selenite for 7 days, then drain the sand in clean water and soak in 0.025% saline. b. Use a stepwise enzymatic hydrolysis method: first add subtilisin and react at 50°C, pH 7.5 for 1 hour, then add flavor protease and react at 55°C, pH 6.5 for 0.5 hour; c. Add 1.2 times the weight of earthworms to the enzymatic hydrolysate to perform the Maillard reaction; (2) Preparation of oyster peptide-peanut protein complex: a. Peanut protein powder and oyster peptide were mixed at a ratio of 1:2, 0.8% pullulan was added, and a composite carrier was formed by Maillard reaction; b loaded composite minerals, composite minerals zinc: selenium molar ratio of 1:1.2; (3) Selenium-enriched treatment of giant salamander protein: The pulsed electric field is used to assist in selenium enrichment, increasing the selenium content by 40%; (4) Preparation of final product: The components are mixed with microencapsulated selenomethionine, and then purified in a purification device to remove larger particle impurities, and finally granulated using a fluidized bed.

9. The method for preparing a ground dragon composite protein powder according to claim 8, wherein: The field strength of the pulse electric field in (3) is 20 kV / cm, and the pulse width is 50 μs.

10. The method for preparing a ground dragon composite protein powder according to claim 8, wherein: The purification device in (4) includes a purification barrel and a plurality of filter components. A filter chamber and a liquid collecting chamber are provided in the purification barrel. The liquid collecting chamber is provided at the lower end of the filter chamber. A plurality of filter cavities are provided in the filter chamber. The plurality of filter components are detachably installed in the filter chamber.

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