A novel device for breaking the cell wall of haematococcus pluvialis

Abstract

The present application relates to the technical field of haematococcus pluvialis breaking wall, specifically, it relates to a new haematococcus pluvialis breaking wall device, including two first breaking wall barrels, a second breaking wall barrel is arranged below the middle of the two first breaking wall barrels, a rolling assembly for secondary haematococcus pluvialis breaking wall is arranged above the middle of the second breaking wall barrel, the rolling assembly includes a shaft, a movable sleeve slidingly connected with the top end of the shaft and four linkage structures regularly arranged in the form of a ring on the sidewall of the movable sleeve. The device performs secondary breaking wall on haematococcus pluvialis by setting the first breaking wall barrel, the second breaking wall barrel and the rolling assembly, and improves the breaking wall effect; meanwhile, the device makes the haematococcus pluvialis after the first breaking wall enter the second breaking wall barrel in batches to avoid too much haematococcus pluvialis entering the filter barrel at a time, which causes the breaking wall to be impossible; in addition, the vibration structure is arranged to knock the filter barrel while the secondary breaking wall is performed, which avoids haematococcus pluvialis blocking the filter barrel and affects the breaking wall filtering efficiency.

Description

Technical Field

[0001] This invention belongs to the field of Haematococcus pluvialis cell wall breaking technology, specifically a novel cell wall breaking device for Haematococcus pluvialis. Background Technology

[0002] Haematococcus pluvialis is a freshwater green algae that is widely used in functional foods, pharmaceuticals, and cosmetics due to its high astaxanthin content. To extract astaxanthin from Haematococcus pluvialis, the cell walls must first be broken down to extract the astaxanthin from the cell sap.

[0003] For example, the invention patent with publication number CN118787991A, in the field of astaxanthin extraction technology, specifically relates to a cell-wall breaking extraction device for astaxanthin from Haematococcus pluvialis, including an extraction chamber, a stirring and crushing mechanism, an upper and lower uniform mechanism, a cell-wall breaking mechanism, a switching mechanism, a separation and extrusion mechanism, and a blocking mechanism. This invention uses a switching mechanism to allow the stirring and crushing mechanism to move downwards during extraction, turning the stirring plates into a vertical state and continuously moving closer and separating them. This allows the stirring plates to better mix, crush, and break the cells of Haematococcus pluvialis in the extract. When moving upwards, the four stirring plates form a complete plane, scooping up the extract from the bottom of the extraction chamber, allowing the extract within the solid-liquid separation plate to uniformly exchange heat with the heating mechanism at the bottom of the extraction chamber. During separation, the four stirring plates form a plane to scrape away the extract from the inner wall of the extraction chamber, and the linked blocking mechanism intermittently extrudes the separation cloth, improving the solid-liquid separation efficiency.

[0004] Based on the above cases and actual situations, we have found the following problems: The amount of Haematococcus pluvialis introduced in a single crushing and cell wall breaking process is usually large. During the crushing and extraction process, a large amount of Haematococcus pluvialis may block the inner wall of the extraction chamber, which not only affects the cell wall breaking effect but also reduces the extraction efficiency. Summary of the Invention

[0005] The purpose of this invention is to provide a novel cell wall breaking device for Haematococcus pluvialis. By setting up a first cell wall breaking tank and a second cell wall breaking tank, secondary cell wall breaking is achieved, thereby improving cell wall breaking efficiency. At the same time, by setting up a crushing component, while the extrusion plate is performing secondary cell wall breaking, the movable sleeve drives the alternating opening and closing of the first and second pistons, so that Haematococcus pluvialis in the first cell wall breaking tank enters the second cell wall breaking tank in batches, avoiding clogging of the filter tank, improving cell wall breaking effect and extraction efficiency, thereby solving the above-mentioned problems of the prior art.

[0006] To achieve the above objectives, the present invention provides a novel cell wall breaking device for Haematococcus pluvialis, comprising two first cell wall breaking barrels arranged symmetrically on the left and right, a second cell wall breaking barrel located below the middle of the two first cell wall breaking barrels, and a crushing component for secondary cell wall breaking of Haematococcus pluvialis located above the middle of the second cell wall breaking barrel.

[0007] In this setup, the red algae undergoes secondary cell disruption through a first and a second cell disruption chamber, thereby enhancing the cell disruption effect.

[0008] The rolling assembly includes a shaft, a movable sleeve slidably connected to the top of the shaft, and four linkage structures arranged in a ring on the side wall of the movable sleeve. The outer sides of the left and right linkage structures are fixedly connected to the first pistons via a first connecting rod, and the outer sides of the front and rear linkage structures are fixedly connected to the second pistons via a second connecting rod. The bottom ring wall of the movable sleeve is symmetrically fixed with pressure rods at the front and rear. A pressing plate is fixedly connected below the pressure rods. A locking rod is fixedly fixed to the inner ring wall of the movable sleeve. The shaft has a locking rod groove on its top outer wall that matches the locking rod.

[0009] In this setup, the rotating shaft drives the movable sleeve to slide up and down, which in turn moves the extrusion plate up and down, extruding the Haematococcus pluvialis inside the filter barrel and breaking its cell walls so that it enters the filter barrel through the filter holes on the bottom surface. At the same time, the filter barrel can also filter out a small number of Haematococcus pluvialis that have not been broken down. When the extrusion plate presses down again, the cell walls are broken down again, improving the cell wall breaking effect and allowing the obtained Haematococcus pluvialis to be directly filtered, reducing the steps of astaxanthin extraction from Haematococcus pluvialis.

[0010] The second blending barrel is fixed with a filter barrel inside. The bottom surface of the first blending barrel is connected to the filter barrel through a connecting pipe that passes through the second blending barrel. A first piston seat is provided inside the connecting pipe. The extrusion plate is provided inside the filter barrel. The bottom of the second blending barrel is provided with a discharge pipe, and a second piston seat is provided inside the discharge pipe.

[0011] In this setup, by setting up a first piston, a second piston, and a linkage structure 310, the red algae are broken down in batches for secondary cell wall breaking, avoiding a large amount of red algae from directly entering the filter canister, which would block the filter pores during filtration and prevent secondary cell wall breaking.

[0012] In the technical solution of the present invention, a fixed seat is fixed inside the first blending barrel, and a frustum-shaped grinding seat is provided below the fixed seat. An opening is provided in the middle of the top surface of the fixed seat. A first motor is fixed outside the top surface of the first blending barrel. A driven rod is coaxially fixed in the middle of the top surface of the grinding seat. The top end of the driven rod protrudes through the top surface of the first blending barrel. The output shaft of the first motor is connected to the top end of the driven rod through two meshing gears.

[0013] In this setup, the first motor drives the driven rod to rotate via gear transmission, which in turn drives the grinding seat to rotate rapidly, causing the protruding parts on the grinding seat and the fixed seat to squeeze and grind the red algae to break the cell wall.

[0014] In the technical solution of the present invention, the vertical section of the first connecting rod passes coaxially downward through the driven rod and the grinding seat on the corresponding side and extends into the communicating pipe. An annular abutment seat is fixed above the corresponding first piston in the communicating pipe. Several regularly distributed abutment rods are fixed on the bottom annular wall of the abutment seat. The upper half of the first piston is conical and the lower half is frustum-shaped. The size and shape of the first piston seat are adapted to the first piston and the first piston seat is vertically connected.

[0015] In this configuration, by setting up an abutment seat and abutment rod, the first piston is prevented from disengaging from the connecting pipe, while also preventing the material from being discharged from the connecting pipe.

[0016] In the technical solution of the present invention, the filter bucket is surrounded by metal plates and the bottom surface is a filter screen. The bottom end of the connecting pipe passes through the top surface of the second wall-breaking bucket and communicates with the middle of the side wall of the filter bucket. The extrusion plate is adapted to the inner diameter of the filter bucket.

[0017] In this setup, by pressing down with the extrusion plate, the red algae in the filter can only be squeezed out from the bottom, ensuring sufficient pressure to break down the algae cells and filter them.

[0018] In the technical solution of the present invention, the second connecting rod passes downward through the top surface of the second wall-breaking barrel, and the second piston seat is fixedly connected to the lower half side wall of the second piston. The upper half of the second piston is frustum-shaped and the lower half is cylindrical. The second piston is located below the second piston seat. The size and shape of the second piston seat are adapted to the upper half of the second piston. The second piston seat is vertically connected.

[0019] In this configuration, when the second connecting rod moves downward, it will drive the second piston to move downward and open the second piston seat, allowing the red algae in the second blending tank to flow out from the discharge pipe.

[0020] In the technical solution of the present invention, the outer wall of the movable sleeve is provided with four movable cavities in a regular annular shape, and the four linkage structures are respectively arranged in the four movable cavities. The linkage structure includes a linkage block, two slide rods and a spring sleeved on the slide rods. The upper and lower ends of the slide rods are respectively fixed to the upper and lower inner walls of the corresponding movable cavities, and the slide rods pass through the corresponding linkage blocks and the two are slidably connected.

[0021] In this configuration, the first and second connecting rods slide up and down by sliding the linkage block along the slide bar.

[0022] In the technical solution of the present invention, the outer walls of the left and right linkage blocks are fixedly connected to the first connecting rod on the corresponding side, the outer walls of the front and rear linkage blocks are fixedly connected to the second connecting rod on the corresponding side, the springs located in the left and right movable cavities are sleeved between the corresponding linkage blocks and the top surface of the movable cavity, and the springs located in the front and rear movable cavities are sleeved between the corresponding linkage blocks and the bottom surface of the movable cavity.

[0023] In this setup, springs positioned at different locations are used to switch the opening and closing states of the first and second pistons, thereby allowing the red algae in the first blending tank to enter the second blending tank in batches.

[0024] In the technical solution of the present invention, the outer wall of the movable sleeve is fixed with a hanging ear between two adjacent movable cavities, and four limiting rods are fixed on the top surface of the second blending bucket. The limiting rods pass through the corresponding hanging ears and are slidably connected. A second motor is fixed on the right side of the middle of the top surface of the second blending bucket. The output shaft of the second motor is connected to the shaft through two meshing gears. The bottom end of the shaft is rotatably connected to the top surface of the second blending bucket. A dust cover is fixed on the middle of the top surface of the second blending bucket.

[0025] In this setup, a limiting rod is used to limit and support the movable sleeve, preventing it from rotating with the shaft. A dust cover is also used to prevent the second connecting rod and pressure rod from coming into contact with the external environment and bringing in debris.

[0026] In the technical solution of the present invention, the second piston seat has symmetrical vibration structures on the top surface ring wall, the two vibration structures are located between the two second connecting rods, the bottom end of the pressure rod passes through the top surface of the dust cover, the top surface of the second wall-breaking barrel, the extrusion plate and the bottom surface of the filter barrel from top to bottom, the pressure rod is fixedly connected to the extrusion plate, and an L-shaped pressure plate is fixed to the bottom end face of the pressure rod.

[0027] In this setting, when the pressure rod moves up and down, it will cause the pressure plate to move up and down synchronously.

[0028] In the technical solution of the present invention, the vibration structure includes a rotating shaft, a bushing rotatably connected to the rotating shaft, and a striking head fixedly connected to the left and right ends of the bushing. The left and right ends of the rotating shaft are fixed to the top surface of the second piston seat by a fixedly connected bracket. The bushing and the rotating shaft are connected by a spring. An inwardly extending plate is fixed in the middle of the side wall of the bushing, and the extending plate corresponds to the position of the pressure plate.

[0029] In this setup, when the pressure plate moves to contact the extended plate, the bushing drives the striking head to rotate, causing the spring to deform and store energy. When the pressure plate separates from the extended plate, the spring releases energy, causing the striking head to hit the bottom of the filter barrel, thus preventing the red algae from clogging the filter holes.

[0030] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0031] 1. In this invention, by setting up a first cell-breaking barrel, a second cell-breaking barrel, and a crushing component, when breaking the cell wall of *Haemaphysalis*, the first motor and the second motor are started. The first motor drives the driven rod and the grinding seat to rotate rapidly, so that the protruding parts on the grinding seat and the fixed seat squeeze and grind the *Haemaphysalis* to achieve a first cell-breaking. Then, the *Haemaphysalis* enters the second cell-breaking barrel through the connecting pipe and undergoes a second cell-breaking under the squeezing between the extrusion plate and the filter barrel, thereby improving the cell-breaking effect.

[0032] 2. In this invention, by setting a first piston, a second piston, and a linkage structure, during the cell wall breaking process, the movable sleeve slides downwards and then upwards under the restriction of the locking groove and locking rod. When the movable sleeve slides down, it drives the first connecting rod and the first piston to descend synchronously and close the connecting pipe. The movable sleeve continues to slide down, driving the second connecting rod and the second piston to descend and open the discharge pipe, so that the red algae in the second cell wall breaking tank are released through the discharge pipe. When the movable sleeve slides up, it first closes the discharge pipe and then opens the connecting pipe, thus repeating the above process in reverse, realizing the alternating opening and closing of the connecting pipe and the discharge pipe, thereby realizing that the red algae in the first cell wall breaking tank enters the second cell wall breaking tank in batches, avoiding clogging of the filter tank, improving the cell wall breaking effect and extraction efficiency.

[0033] 3. In this invention, by setting a vibration structure, during the cell wall breaking process, when the pressure plate moves to contact the outer extension plate, the bushing drives the striking head to rotate, causing the spring to deform and store energy. When the pressure plate separates from the outer extension plate, the spring releases energy and drives the striking head to strike the bottom surface of the filter barrel, thus preventing the red algae from clogging the filter holes of the filter barrel. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0035] Figure 2 This is a schematic diagram of the interior of the first blending bucket of the present invention;

[0036] Figure 3 This is a schematic diagram of the interior of the second blending tank in this invention;

[0037] Figure 4 This is a schematic diagram of the interior of the filter box in this invention;

[0038] Figure 5 This is an exploded view of the crushing component in this invention;

[0039] Figure 6 This is a schematic diagram of the interior of the connecting tube in this invention;

[0040] Figure 7 For the present invention Figure 6 Exploded view of the central connecting pipe;

[0041] Figure 8 This is a cross-sectional view of the discharge pipe in this invention;

[0042] Figure 9 This is an exploded view of the filter box in this invention;

[0043] Figure 10 This is a schematic diagram of the vibration structure in this invention;

[0044] Figure 11 This is an exploded view of the vibration structure in this invention.

[0045] Explanation of reference numerals in the attached figures:

[0046] 100. First blending barrel; 101. Fixed base; 102. Grinding base; 103. First motor; 104. Driven rod; 105. Connecting pipe; 105a. Abutment seat; 106. First piston seat;

[0047] 200. Second blending tank; 201. Filter tank; 202. Discharge pipe; 203. Second piston seat;

[0048] 300. Compactor assembly; 301. Second motor; 302. Shaft; 302a. Locking rod groove; 303. Movable sleeve; 303a. Locking rod; 303b. Movable cavity; 304. First connecting rod; 304a. First piston; 305. Second connecting rod; 305a. Second piston; 306. Limiting rod; 307. Pressure rod; 307a. Pressure plate; 308. Extrusion plate; 310. Linkage structure; 311. Linkage block; 312. Slide rod; 313. Spring; 320. Vibration structure; 321. Bracket; 322. Rotating shaft; 323. Clockwork spring; 324. Bushing; 325. Outer plate; 326. Striking head;

[0049] 400. Dust cover. Detailed Implementation

[0050] The specific embodiments of the present invention will now be described in detail with reference to the accompanying drawings, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0051] Unless otherwise expressly stated, throughout this specification the term “comprising” or variations thereof such as “including” or “comprises”, etc., shall be understood to include the stated elements or components, without excluding other elements or other components.

[0052] Reference Figures 1-11 As shown, this embodiment provides a technical solution:

[0053] The novel cell wall breaking device for Haematococcus pluvialis in this invention includes two first cell wall breaking tanks 100 arranged symmetrically on the left and right, a second cell wall breaking tank 200 located below the middle of the two first cell wall breaking tanks 100, and a crushing component 300 for secondary cell wall breaking of Haematococcus pluvialis located above the middle of the second cell wall breaking tank 200. The Haematococcus pluvialis is subjected to secondary cell wall breaking through the first cell wall breaking tanks 100 and the second cell wall breaking tank 200, thereby improving the cell wall breaking effect.

[0054] The compaction assembly 300 includes a shaft 302, a movable sleeve 303 slidably connected to the top of the shaft 302, and four linkage structures 310 arranged in a ring on the side wall of the movable sleeve 303. The outer sides of the left and right linkage structures 310 are fixedly connected to the first piston 304a via a first connecting rod 304, and the outer sides of the front and rear linkage structures 310 are fixedly connected to the second piston 305a via a second connecting rod 305. Pressure rods 307 are symmetrically fixed to the bottom annular wall of the movable sleeve 303. A pressing plate 308 is fixedly connected below the pressure rods 307. A locking rod 303a is fixed to the inner annular wall of the movable sleeve 303. The shaft 302 has a locking rod groove 302a near the top outer wall that matches the locking rod 303a. The locking rod groove 302a is a three-quarter spiral from top to bottom. The device consists of a circular arc groove and an arc groove that spirals counterclockwise by a quarter turn from top to bottom. When the shaft 302 rotates counterclockwise, the movable sleeve 303 slides down first and then up under the restriction of the locking groove 302a and the locking rod 303a, while driving the extrusion plate 308 to move up and down. The filter barrel 201 is fixed inside the second wall-breaking barrel 200. When the extrusion plate 308 moves down inside the filter barrel 201, it will squeeze the red algae in the filter barrel 201 to break the wall and enter the filter barrel 201 from the filter hole at the bottom of the filter barrel 201. At the same time, the filter barrel 201 can also filter out a few red algae that have not broken the wall. When the extrusion plate 308 squeezes down again, the wall will be broken again, improving the wall-breaking effect. At the same time, it can directly filter the obtained red algae, reducing the extraction steps of astaxanthin in red algae.

[0055] The bottom of the first blending tank 100 is connected to the second blending tank 200 and the filter tank 201 via a connecting pipe 105. A first piston seat 106 is located inside the connecting pipe 105. A pressing plate 308 is located inside the filter tank 201. The bottom of the second blending tank 200 has a discharge pipe 202, inside which is a second piston seat 203. When the movable sleeve 303 slides downwards, the first piston 304a slides downwards synchronously under the action of the linkage structure 310, gradually contacting the first piston seat 106 and closing the connecting pipe 105. The movable sleeve 303 continues to slide downwards, and the second piston 305a gradually separates from the second piston seat 203 under the action of the linkage structure 310, thus opening the discharge pipe 202. This allows the red algae that have undergone secondary cell wall breaking in the second cell wall breaking tank 200 to flow out and be collected. By setting the first piston 304a, the second piston 305a, and the linkage structure 310, secondary cell wall breaking is carried out in batches, avoiding a large amount of red algae from directly entering the filter tank 201, which would cause the filter holes of the filter tank 201 to be directly blocked during filtration, thus preventing secondary cell wall breaking.

[0056] Please see Figures 1-2 As shown, a fixed base 101 is fixed inside the first blending tank 100. A frustum-shaped grinding seat 102 is located below the fixed base 101. A gap is left between the fixed base 101 and the grinding seat 102 for the entry of *Hylocereus undatus*. Simultaneously, protrusions are alternately arranged on the opposing surfaces of the grinding seat 102 and the fixed base 101. An opening is provided in the center of the top surface of the fixed base 101. It should be noted that *Hylocereus undatus* enters directly from the first blending tank 100 through a pipe into the gap opening between the top surfaces of the fixed base 101 and the grinding seat 102, preventing *Hylocereus undatus* residue from remaining on the top surface of the fixed base 101. On the ring wall, a first motor 103 is fixed to the outside of the top surface of the first wall-breaking barrel 100, and a driven rod 104 is coaxially fixed to the middle of the top surface of the grinding seat 102. The top end of the driven rod 104 protrudes from the top surface of the first wall-breaking barrel 100. The output shaft of the first motor 103 is connected to the top end of the driven rod 104 through two meshing gears. The first motor 103 drives the driven rod 104 to rotate through gear transmission, which in turn drives the grinding seat 102 to rotate rapidly, so that the protruding parts on the grinding seat 102 and the fixed seat 101 squeeze and grind the red algae to achieve wall breaking.

[0057] Please see Figures 3-5 As shown, the outer wall of the movable sleeve 303 is regularly arranged in a ring shape with four movable cavities 303b. Four linkage structures 310 are correspondingly arranged in the four movable cavities 303b. The linkage structure 310 includes a linkage block 311, two slide rods 312 and a spring 313 sleeved on the slide rod 312. The upper and lower ends of the slide rod 312 are fixed to the upper and lower inner walls of the corresponding movable cavity 303b, respectively. The slide rod 312 passes through the corresponding linkage block 311 and the two are slidably connected. When the movable sleeve 303 moves downward, the linkage block 311 will move according to the position of the spring 313, thereby controlling the opening and closing of the first piston 304a and the second piston 305a.

[0058] Specifically, the outer walls of the left and right linkage blocks 311 are fixedly connected to the corresponding first connecting rod 304, and the outer walls of the front and rear linkage blocks 311 are fixedly connected to the corresponding second connecting rod 305. Springs 313 located in the left and right movable cavities 303b are sleeved between the corresponding linkage block 311 and the top surface of the movable cavity 303b, and springs 313 located in the front and rear movable cavities 303b are sleeved between the corresponding linkage block 311 and the bottom surface of the movable cavity 303b. When the movable sleeve 303 is at its highest point, the springs 313 on both sides are in a stretched state under the gravity of the first connecting rod 304, the first piston 304a separates from the first piston seat 106, the front and rear springs 313 are in a compressed state, and exert an upward pulling force on the second connecting rod 305, causing the second piston 305a to press tightly against the second piston seat 203. At this time, the connecting pipe 105 is in an open state, the discharge pipe 202 is in a closed state, and the contents of the first crushing barrel 100... The *Hylocereus undatus* enters the second cell-breaking tank 200 through the connecting pipe 105. When the movable sleeve 303 moves downward from its highest point, the springs 313 on both sides decrease in height, and the first connecting rod 304 descends synchronously with the movable sleeve 303 until the first piston 304a contacts the first piston seat 106. At this time, the extrusion plate 308 moves to be level with the upper part of the connection between the connecting pipe 105 and the filter tank 201. When the movable sleeve 303 continues to slide down to the lower part of the connection between the connecting pipe 105 and the filter tank 201, the first piston 304a is blocked by the first piston seat 106 and cannot move down. The springs 313 on both sides are compressed, the connecting pipe 105 is closed, and the second piston 305a gradually separates from the second piston seat 203, so that the discharge pipe 202 gradually opens and releases the *Hylocereus undatus* after secondary cell-breaking. When the movable sleeve 303 moves upward, the above process is repeated in reverse. By repeating this process, the *Hylocereus undatus* can be broken into batches for secondary cell-breaking.

[0059] In addition, the outer wall of the movable sleeve 303 is fixed with lugs between two adjacent movable cavities 303b. Four limiting rods 306 are fixed on the top surface of the second blending barrel 200. The limiting rods 306 pass through the corresponding lugs and are slidably connected. The limiting rods 306 limit and support the movable sleeve 303 to prevent it from rotating with the shaft 302. A second motor 301 is fixed on the right side of the middle of the top surface of the second blending barrel 200. The output shaft of the second motor 301 is connected to the shaft 302 through two meshing gears. The bottom end of the shaft 302 is coaxially rotatably connected to the top surface of the second blending barrel 200. The gears of the second motor 301 drive the shaft 302 to rotate. A dust cover 400 is fixed on the middle of the top surface of the second blending barrel 200. By setting the dust cover 400, the contact parts of the second connecting rod 305 and the pressure rod 307 with the inside of the second blending barrel 200 are prevented from contacting the external environment and bringing in debris.

[0060] Please see 3- Figure 7As shown, the vertical section of the first connecting rod 304 passes coaxially downward through the corresponding driven rod 104 and grinding seat 102 and extends into the connecting pipe 105, preventing the first connecting rod 304 from blocking the rotation of the driven rod 104. An annular abutment seat 105a is fixed inside the connecting pipe 105 above the corresponding first piston 304a. Several regularly distributed abutment rods are fixed on the bottom annular wall of the abutment seat 105a. By setting the abutment seat 105a and the abutment rods, the first piston 304a is prevented from detaching from the connecting pipe 105, while the material is not blocked from flowing out of the connecting pipe 105. The upper half of the first piston 304a is conical and the lower half is frustum-shaped. The first piston seat 106 is adapted to the size and shape of the first piston 304a and is vertically connected. When the first piston 304a is not closed, the red algae enter the second wall-breaking tank 200 through the first piston seat 106.

[0061] Please see Figure 8 As shown, the second connecting rod 305 passes downward through the top surface of the second blending tank 200, and is fixedly connected to the lower half of the side wall of the second piston 305a via the second piston seat 203. The upper half of the second piston 305a is frustum-shaped and the lower half is cylindrical. The second piston 305a is located below the second piston seat 203. The size and shape of the second piston seat 203 are adapted to the upper half of the second piston 305a. The second piston seat 203 is vertically connected. When the second connecting rod 305 moves downward, it will drive the second piston 305a to move downward and open the second piston seat 203, so that the red algae in the second blending tank 200 can flow out from the discharge pipe 202.

[0062] Please see Figure 9 As shown, the filter bucket 201 is surrounded by metal plates and has a filter screen on the bottom, so that when the extrusion plate 308 is pressed down, the red algae in the filter bucket 201 can only be squeezed out from the bottom, ensuring sufficient pressure to break the algae wall and filter. The bottom end of the connecting pipe 105 passes through the top surface of the second wall-breaking bucket 200 and connects to the middle of the side wall of the filter bucket 201. The extrusion plate 308 is adapted to the inner diameter of the filter bucket 201. A sealing ring is provided between the pressure plate 307a and the filter bucket 201 to ensure airtightness.

[0063] Please see Figures 10-11 As shown, the second piston seat 203 has symmetrical vibration structures 320 on the top annular wall. The two vibration structures 320 are located between the two second connecting rods 305. The bottom end of the pressure rod 307 passes through the top surface of the dust cover 400, the top surface of the second wall-breaking barrel 200, the extrusion plate 308, and the bottom surface of the filter barrel 201 from top to bottom. The pressure rod 307 is fixedly connected to the extrusion plate 308. An L-shaped pressure plate 307a is fixed to the bottom end face of the pressure rod 307. A sealing ring is provided between the pressure rod 307 and the bottom surface of the filter barrel 201 to ensure sealing. When the pressure rod 307 moves up and down, it will drive the pressure plate 307a to move up and down synchronously.

[0064] Specifically, the vibration structure 320 includes a rotating shaft 322, a bushing 324 rotatably connected to the rotating shaft 322, and striking heads 326 fixedly connected to the left and right ends of the bushing 324. The left and right ends of the rotating shaft 322 are fixed to the top surface of the second piston seat 203 by a bracket 321. An inwardly facing extension plate 325 is fixed in the middle of the side wall of the bushing 324. The bushing 324 and the rotating shaft 322 are connected by a spring 323. The extension plate 325 corresponds to the position of the pressure plate 307a. When the pressure plate 307a moves to contact the extension plate 325, the bushing 324 drives the striking heads 326 to rotate, causing the spring 323 to deform and store energy. When the pressure plate 307a separates from the extension plate 325, the spring 323 releases energy and drives the striking heads 326 to strike the bottom surface of the filter barrel 201, preventing the red algae from clogging the filter holes of the filter barrel 201.

[0065] The working principle of the novel cell wall breaking device for Haematococcus pluvialis in this invention is as follows:

[0066] When breaking the cell wall of Haematococcus pluvialis, the Haematococcus pluvialis solution is directly introduced into the space between the fixed seat 101 and the grinding seat 102 through the first cell breaking tank 100 via the pipe. At the same time, the first motor 103 and the second motor 301 are started. The first motor 103 drives the driven rod 104 and the grinding seat 102 to rotate rapidly, so that the protruding parts on the grinding seat 102 and the fixed seat 101 squeeze and grind the Haematococcus pluvialis to achieve cell wall breaking in one step.

[0067] The second motor 301 drives the shaft 302 to rotate counterclockwise. The movable sleeve 303, restricted by the locking groove 302a and the locking rod 303a, first slides downwards and then upwards. When the movable sleeve 303 is at its highest point, the springs 313 on both sides are in a stretched state under the gravity of the first connecting rod 304. The first piston 304a separates from the first piston seat 106, and the two springs 313 are in a compressed state, providing an upward pulling force to the second connecting rod 305, causing the second piston 305a to press tightly against the second piston seat 203. At this time, the connecting pipe 105 is open, and the discharge pipe 202 is closed. The red algae in the first blending tank 100 enters the second blending tank 200 through the connecting pipe 105. When the movable sleeve 303 moves downwards from its highest point, the springs on both sides... As the height of 313 decreases, the first connecting rod 304 descends synchronously with the movable sleeve 303 until the first piston 304a contacts the first piston seat 106. At this time, the extrusion plate 308 moves to be level with the upper part of the connection between the connecting pipe 105 and the filter barrel 201. When the movable sleeve 303 continues to slide down to the lower part of the connection between the connecting pipe 105 and the filter barrel 201, the first piston 304a is blocked by the first piston seat 106 and cannot move down. The springs 313 on both sides are compressed, the connecting pipe 105 is closed, and the second piston 305a gradually separates from the second piston seat 203, so that the discharge pipe 202 gradually opens to release the red algae after secondary cell wall breaking. When the movable sleeve 303 moves up, the above process is repeated in reverse. By repeating this process, the red algae can be broken in batches for secondary cell wall breaking.

[0068] During the cell wall breaking process, when the pressure plate 307a moves to contact the extended plate 325, the bushing 324 drives the striking head 326 to rotate, causing the spring 323 to deform and store energy. When the pressure plate 307a separates from the extended plate 325, the spring 323 releases energy and drives the striking head 326 to strike the bottom of the filter barrel 201, preventing the red algae from clogging the filter holes of the filter barrel 201.

[0069] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the specification and its equivalents.

Claims

1. A novel cell wall breaking device for Haematococcus pluvialis, comprising two first cell wall breaking tanks arranged symmetrically on the left and right, characterized in that: A second cell is provided below the middle of the two first cell-breaking tanks, and a crushing component for secondary cell-breaking of *Rhodotorula glutinis* is provided above the middle of the second cell-breaking tank. The rolling assembly includes a shaft, a movable sleeve slidably connected to the top of the shaft, and four linkage structures arranged in a ring on the side wall of the movable sleeve. The outer sides of the left and right linkage structures are fixedly connected to the first pistons via a first connecting rod, and the outer sides of the front and rear linkage structures are fixedly connected to the second pistons via a second connecting rod. The bottom ring wall of the movable sleeve is symmetrically fixed with pressure rods at the front and rear. A pressing plate is fixedly connected below the pressure rods. A locking rod is fixedly fixed to the inner ring wall of the movable sleeve. The shaft has a locking rod groove on its top outer wall that matches the locking rod. The second blending barrel is fixed with a filter barrel inside. The bottom surface of the first blending barrel is connected to the filter barrel through a connecting pipe that passes through the second blending barrel. A first piston seat is provided inside the connecting pipe. The extrusion plate is provided inside the filter barrel. The bottom of the second blending barrel is provided with a discharge pipe, and a second piston seat is provided inside the discharge pipe. A fixed base is fixed inside the first blending barrel, and a frustum-shaped grinding seat is provided below the fixed base. An opening is provided in the middle of the top surface of the fixed base. A first motor is fixed outside the top surface of the first blending barrel. A driven rod is coaxially fixed in the middle of the top surface of the grinding seat. The top end of the driven rod protrudes through the top surface of the first blending barrel. The output shaft of the first motor is connected to the top end of the driven rod through two meshing gears. The vertical section of the first connecting rod passes coaxially downward through the driven rod and the grinding seat on the corresponding side and extends into the connecting pipe. An annular abutment seat is fixed above the corresponding first piston in the connecting pipe. Several regularly distributed abutment rods are fixed on the bottom annular wall of the abutment seat. The upper half of the first piston is conical and the lower half is frustum-shaped. The size and shape of the first piston seat are adapted to the first piston and the first piston seat is through the top and bottom. The filter bucket is surrounded by metal plates and has a filter screen on the bottom. The bottom end of the connecting pipe passes through the top surface of the second wall-breaking bucket and connects with the middle of the side wall of the filter bucket. The extrusion plate is adapted to the inner diameter of the filter bucket. The second connecting rod passes downward through the top surface of the second wall-breaking barrel, and the second piston seat is fixedly connected to the lower half of the side wall of the second piston. The upper half of the second piston is frustum-shaped and the lower half is cylindrical. The second piston is located below the second piston seat. The size and shape of the second piston seat are adapted to the upper half of the second piston. The second piston seat is vertically connected. The outer wall of the movable sleeve is provided with four movable cavities in a ring-shaped regular pattern. The four linkage structures are respectively arranged in the four movable cavities. The linkage structure includes a linkage block, two slide rods and a spring sleeved on the slide rods. The upper and lower ends of the slide rods are respectively fixed to the upper and lower inner walls of the corresponding movable cavities. The slide rods pass through the corresponding linkage blocks and the two are slidably connected. The outer walls of the left and right linkage blocks are fixedly connected to the first connecting rod on the corresponding side, and the outer walls of the front and rear linkage blocks are fixedly connected to the second connecting rod on the corresponding side. The springs located in the left and right movable cavities are sleeved between the corresponding linkage blocks and the top surface of the movable cavity, and the springs located in the front and rear movable cavities are sleeved between the corresponding linkage blocks and the bottom surface of the movable cavity. The outer wall of the movable sleeve is fixed with a hanging lug between each of the two adjacent movable cavities. Four limiting rods are fixed on the top surface of the second blending bucket. The limiting rods pass through the corresponding hanging lugs and are slidably connected. A second motor is fixed on the right side of the middle of the top surface of the second blending bucket. The output shaft of the second motor is connected to the shaft through two meshing gears. The bottom end of the shaft is rotatably connected to the top surface of the second blending bucket. A dust cover is fixed on the middle of the top surface of the second blending bucket.

2. The novel cell wall breaking device for Haematococcus pluvialis as described in claim 1, characterized in that: The second piston seat has symmetrical vibration structures on the top annular wall. The two vibration structures are located between the two second connecting rods. The bottom end of the pressure rod passes through the top surface of the dust cover, the top surface of the second wall-breaking barrel, the extrusion plate, and the bottom surface of the filter barrel from top to bottom. The pressure rod is fixedly connected to the extrusion plate, and an L-shaped pressure plate is fixed to the bottom end face of the pressure rod.

3. The novel cell wall breaking device for Haematococcus pluvialis as described in claim 2, characterized in that: The vibration structure includes a rotating shaft, a bushing rotatably connected to the rotating shaft, and a striking head fixedly connected to the left and right ends of the bushing. The left and right ends of the rotating shaft are fixed to the top surface of the second piston seat by a fixed bracket. The bushing and the rotating shaft are connected by a spring. An inwardly extending plate is fixed in the middle of the side wall of the bushing, and the extending plate corresponds to the position of the pressure plate.

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