Haematococcus pluvialis mutant strain for efficiently producing astaxanthin and application of haematococcus pluvialis mutant strain
By inducing ARTP mutagenesis, β-ionone domestication, and glycerol domestication in Haematococcus pluvialis, and combining a three-stage culture process of heterotrophic growth in the absence of light, short-term weak light activation, and salt stress induction, the problem of low efficiency in the fermentation production of astaxanthin from Haematococcus pluvialis was solved, and efficient and stable astaxanthin production was achieved, which is suitable for industrial application.
Patent Information
- Application Number
- CN202610714685.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-22
- Publication Date
- 2026-06-26
AI Technical Summary
The current method for producing astaxanthin by fermentation of Haematococcus pluvialis has low efficiency and yield, which cannot meet the needs of industrialization. It also has problems such as high carbon source cost, high energy consumption and complex dissolved oxygen control.
A mutant strain of Haematococcus pluvialis C01 with high astaxanthin production was obtained by ARTP mutagenesis, β-ionone domestication and glycerol domestication. The strain was then cultured using a three-stage process of heterotrophic growth in the dark, short-term weak light activation and salt stress induction in the dark. Glycerol was used as the carbon source to avoid high concentration acetic acid stress and high dissolved oxygen induction, and high accumulation was achieved by combining salt stress and nitrogen starvation.
It achieves high-density biomass accumulation and high astaxanthin content in a closed fermentation tank, significantly reducing production costs, with a short production cycle, suitable for large-scale industrial production, and strong batch-to-batch stability.