Centrifugal plasma separation cup and separation method thereof

Abstract

The centrifugal plasma separation cup comprises a cup body, a plasma collection sleeve of an arc-shaped structure is installed at an inlet of the cup body, a gland used for fixing the plasma collection sleeve is arranged on an opening of the cup body, a mandrel pipe is installed in the cup body, and the mandrel pipe is provided with an upper opening and a lower opening after penetrating through the plasma collection sleeve and the gland; the gland is provided with a sealing piece used for sealing the mandrel pipe, and the sealing piece is sleeved with a protective cover. Due to the fact that the plasma collecting sleeve is arranged in the cup body, secondary separation of plasma can be achieved, due to the structure of the plasma collecting sleeve, the volume of blood entering the separation cup at a time can be increased, the plasma separated at a time is more in component, the required time of the total amount is shortened, the plasma collecting sleeve is shortened to 20 mm + / -1 mm, and the size of the plasma collecting sleeve is reduced. Therefore, when the single circulation stops and the collected plasma is in contact with the plasma collecting surface, the friction force is reduced, the generation of foams in the plasma collecting process is reduced, and the plasma quality is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of blood separation, in particular to a centrifugal plasma separation cup and a separation method thereof. Background Art

[0002] Human blood products, due to their unique biological properties, have demonstrated strong therapeutic effects in long-term clinical practice and are increasingly recognized and valued in modern medicine. Blood products are a crucial resource in healthcare. The collection of healthy human plasma, a key raw material for blood product production, directly impacts their production, supply, and clinical use. Over decades of development, domestic blood product manufacturers have achieved significant improvements in production processes and equipment.

[0003] Plasmapheresis is a technique in which whole blood is collected from the human body and the red blood cells and other formed elements are returned to the donor, while only the plasma is retained. Currently, a centrifugal plasma separator is used to separate the plasma. However, due to friction and agitation of the plasma during centrifugation, this device produces a lot of foam in the collected plasma, which reduces the plasma quality and protein and factor IIIV content. Summary of the Invention

[0004] In view of the above-mentioned defects or shortcomings, the object of the present invention is to provide a centrifugal plasma separation cup and a separation method thereof.

[0005] In order to achieve the above objects, the technical solution of the present invention is:

[0006] A centrifugal plasma separation cup comprises: a cup body, an arc-shaped plasma collection sleeve is installed at the inlet of the cup body, a pressure cover for fixing the plasma collection sleeve is provided on the cup body opening, a core shaft tube is installed in the cup body, and the core shaft tube is provided with upper and lower openings after passing through the plasma collection sleeve and the pressure cover; a sealing member for sealing the core shaft tube is provided on the pressure cover, and a protective cover is provided on the sealing member.

[0007] The slurry collecting sleeve comprises a slurry collecting body, an upper edge of the slurry collecting body is provided with a slurry outlet seam, an upper slurry collecting concave surface is provided in the slurry collecting body, and a slurry collecting surface is provided on the outer surface of the slurry collecting body.

[0008] The upper slurry collecting concave surface is a concave structure, and the angle between the upper slurry collecting concave surface and the inner wall of the slurry collecting surface is in the range of degrees to degrees.

[0009] A core shaft seat is installed between the pressure cover and the fixed slurry collecting sleeve. The upper end of the core shaft seat is provided with upper and lower openings after passing through the slurry collecting sleeve and the pressure cover. The core shaft tube is installed at the lower end of the core shaft seat. The core shaft seat is sleeved with a core shaft upper seat.

[0010] The core shaft seat includes: a core shaft seat skirt, a core shaft seat skirt surface is installed on the core shaft seat skirt, a core shaft seat three-point upper plane is provided on the core shaft seat skirt surface, an output pipe is clamped on the core shaft seat three-point upper plane, and the upper and lower ports are provided on the output pipe.

[0011] The planes on the three points of the core shaft seat are three evenly arranged support ribs.

[0012] The core shaft upper seat comprises: a core shaft upper seat skirt and a core shaft upper seat lower plane, and the core shaft upper seat lower plane is sleeved on the core shaft seat skirt surface.

[0013] The core shaft seat skirt and the core shaft upper seat skirt form a smooth pulp receiving gap, and the three supporting ribs on the plane at three points of the core shaft seat form a pulp outlet gap between the core shaft seat and the core shaft upper seat.

[0014] The sealing member includes: a graphite ring, a rubber bowl, and a ceramic ring A which are sequentially installed on the gland from bottom to top.

[0015] The following steps are involved:

[0016] 1) Whole blood containing anticoagulant is input from the upper port and enters the cup body through the core shaft tube;

[0017] 2) The cup body is mounted on a plasma collection device and rotates at high speed;

[0018] 3) The slurry collecting sleeve, gland and seal are fixedly connected and then rotated rapidly to form a dynamic sealing system;

[0019] 4) As the blood spins at high speed in the cup, cells separate. Red blood cells are thrown to the outer layer by centrifugal force, while the lighter plasma is in the inner layer. The plasma in the inner layer gradually contacts the plasma collection sleeve and overflows upward along the plasma collection sleeve.

[0020] 5) The plasma overflowing into the arc-shaped surface of the plasma collecting sleeve is centrifuged again. As the amount of plasma increases, the plasma overflows along the lower opening of the protective cover and the collected plasma is collected.

[0021] 6) As the plasma is collected, the red blood cells will gradually move to the middle until they reach the overflow port under the protective cover and the separation stops.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The present invention provides a centrifugal plasma separation cup and a separation method thereof. Since a plasma collecting sleeve is provided in the cup body, secondary separation of plasma can be achieved. Due to the structure of the plasma collecting sleeve, the blood volume entering the separation cup at a single time can be increased, more plasma components can be separated at a single time, and the required time for the total amount is reduced. By shortening the plasma collecting sleeve to 20mm±1mm, the friction force when a single circulation stops and when the collected plasma contacts the plasma collecting surface is reduced, the generation of foam during the plasma collection process is reduced, and the plasma quality is improved.

[0024] Furthermore, by reducing the outer diameter of the core skirt and the core upper skirt to 38mm±1mm, the plasma separation time is prolonged, thereby improving the plasma quality. By designing the upper plasma collection concave surface (with an angle of 65°±5° to the plasma collection body), the plasma undergoes a second, brief separation on the upper plasma collection concave surface, thereby improving the plasma quality. In addition, by increasing the plasma outlet gap to 2mm±0.5mm, the plasma collection speed is increased, reducing the total amount of time required. By increasing the gap between the core skirt and the core upper skirt to more than 0.7mm, the plasma collection speed is increased, reducing the total amount of time required. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic structural diagram of the centrifugal plasma separation cup of the present invention;

[0026] Figure 2 yes Figure 1 Schematic cross-sectional view of ;

[0027] Figure 3 This is a schematic diagram of the structure of the centrifugal plasma separation cup and plasma collection sleeve of the device of the present invention;

[0028] Figure 4 yes Figure 3 Schematic cross-sectional view of ;

[0029] Figure 5 This is a schematic structural diagram of the spindle seat of the centrifugal plasma separation cup of the present invention;

[0030] Figure 6 This is a schematic diagram of the structure of the centrifugal plasma separation cup core shaft upper seat of the present invention;

[0031] Figure 7 This is a schematic diagram of the combined structure of the centrifugal plasma separation cup core shaft upper seat and the core shaft seat of the present invention.

[0032] Figure 8 yes Figure 7 Schematic cross-sectional view of ;

[0033] Figure 9 The figure is a schematic cross-sectional view of the structure of the centrifugal plasma separation cup of the present invention when in use.

[0034] In the figure, 1000 protective cover, 2000 cup body, 3000 slurry collecting sleeve, 3001 upper slurry collecting concave surface, 3002, slurry collecting surface, 3003 slurry collecting body, 3004 slurry outlet gap, 4000 mandrel seat, 4001 mandrel seat three-point upper plane, 4002 mandrel seat skirt, 4003 mandrel seat surface, 5000 mandrel upper seat, 5001 mandrel upper seat skirt, 5002 mandrel upper seat lower plane, 6000 pressure cover, 7000 mandrel tube, 8000 graphite ring, 9000 rubber bowl, A000 ceramic ring. DETAILED DESCRIPTION

[0035] The present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the embodiments described herein are only some embodiments of the present invention, not all embodiments. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are intended to fall within the scope of protection of the present invention.

[0036] like Figure 1 、 2 As shown, the present invention provides a centrifugal plasma separation cup, comprising: a cup body 2000, a slurry collecting sleeve 3000 with an arc-shaped structure is installed at the entrance of the cup body 2000, a pressure cover 6000 for fixing the slurry collecting sleeve 3000 is provided on the mouth of the cup body 2000, a core shaft tube 7000 is installed in the cup body 2000, and the core shaft tube 7000 is provided with an upper and a lower opening after passing through the slurry collecting sleeve 3000 and the pressure cover 6000; a sealing member for sealing the core shaft tube 7000 is provided on the pressure cover 6000, and a protective cover 1000 is provided on the sealing member.

[0037] The slurry collecting sleeve 3000 comprises an upper slurry collecting concave surface 3001, a slurry collecting surface 3002, a slurry collecting body 3003 and a slurry outlet slit 3004. The mandrel seat 4000 comprises a mandrel seat three-point upper plane 4001, a mandrel seat skirt 4002 and a mandrel seat skirt surface 4003. The mandrel upper seat 5000 comprises a mandrel upper seat skirt 5001 and a mandrel upper seat lower plane 5002.

[0038] Specifically, if Figure 3 、 4 As shown, the slurry collecting sleeve 3000 includes a slurry collecting body 3003, a slurry outlet slit 3004 provided on the upper edge of the slurry collecting body 3003, an upper slurry collecting concave surface 3001 provided inside the slurry collecting body 3003, and a slurry collecting surface 3002 provided on the outer surface of the slurry collecting body 3003. Furthermore, the upper slurry collecting concave surface 3001 is a concave structure, and the angle between the upper slurry collecting concave surface 3001 and the inner wall of the slurry collecting body 3003 is in the range of 60-70 degrees.

[0039] The outer diameter of the slurry collecting surface 3002 of the slurry collecting sleeve 3000 is designed to be 42mm±1mm; the slurry collecting body 3003 of the slurry collecting sleeve 3000 is designed to have a length of 20mm±2mm; and the slurry outlet slit 3004 of the slurry collecting sleeve 3000 is designed to have a width of 2mm±0.5mm.

[0040] Preferably, if Figure 5-8 As shown, a mandrel seat 4000 is installed between the pressure cover 6000 and the fixed slurry collecting sleeve 3000. The upper end of the mandrel seat 4000 is provided with an upper and lower opening after passing through the slurry collecting sleeve 3000 and the pressure cover 6000. The mandrel tube 7000 is installed at the lower end of the mandrel seat 4000, and the mandrel upper seat 5000 is mounted on the mandrel seat 4000. The mandrel seat 4000 includes: a mandrel seat skirt 4002, a mandrel seat skirt surface 4003 is installed on the mandrel seat skirt surface 4003, a mandrel seat three-point upper plane 4001 is provided on the mandrel seat three-point upper plane 4001, an output pipe 4004 is clamped on the mandrel seat three-point upper plane 4001, the upper and lower openings are provided on the output pipe, and the mandrel seat three-point upper plane 4001 is three evenly arranged support ribs.

[0041] In the present invention, the outer diameters of the core shaft skirt 4002 and the core shaft upper skirt (5001) are designed to be 38mm±1mm; the three-point upper plane 4001 of the core shaft seat is designed with three support ribs with a height greater than 0.7mm to ensure that the slurry outlet gap between the core shaft seat 4000 and the core shaft upper seat 5000 is greater than 0.7mm.

[0042] The mandrel upper seat 5000 includes: a mandrel upper seat skirt 5001 and a mandrel upper seat lower plane 5002. The mandrel upper seat lower plane 5002 is sleeved on the mandrel seat skirt surface 4003. The mandrel seat skirt 4002 and the mandrel upper seat skirt 5001 form a smooth slurry collection gap. The three supporting ribs of the mandrel seat three-point upper plane 4001 ensure that the slurry discharge gap between the mandrel seat 4000 and the mandrel upper seat 5000 is greater than 0.7 mm.

[0043] Further preferably, the sealing element comprises: a graphite ring 8000, a rubber cup 9000, and a ceramic ring A000, which are sequentially mounted on the gland 6000 from bottom to top. The ceramic ring A000 is adhesively fixed to the upper portion of the gland 6000, the graphite ring 8000 is sleeved on the lower portion of the rubber cup 9000, and the protective cover 1000 is adhesively fixed to the output pipe.

[0044] The present invention also provides a separation method using a centrifugal plasma separation cup, comprising the following steps:

[0045] 1) Whole blood containing anticoagulant is input from the upper port and enters the cup body 2000 through the core shaft tube 7000;

[0046] 2) The cup body 1000 is mounted on a plasma collection device and rotates at high speed;

[0047] 3) The slurry collecting sleeve 3000, the gland 6000 and the sealing element are fixedly connected and then rotated rapidly to form a dynamic sealing system;

[0048] Since the slurry collecting sleeve 3000, the pressure cover 6000 and the ceramic ring A000 are fixedly bonded to the separation cup and rotate at high speed, the graphite ring 8000 cooperates with the rubber bowl 9000 to form a dynamic sealing system with the ceramic ring A000.

[0049] 4) As the blood rotates at high speed in the cup body 2000, cells are separated. Red blood cells are thrown to the outer layer by centrifugal force, while the lighter plasma is in the inner layer. The plasma in the inner layer gradually contacts the plasma collection sleeve 3000 and overflows upward along the plasma collection sleeve 3000.

[0050] Specifically, as the blood enters, the cup body 3000 rotates at high speed, and the blood is gradually stratified inside the cup body 3000. The heavier red blood cells are thrown into the outer layer by centrifugal force, while the lighter plasma cells are in the inner layer; as the blood continues to enter, the plasma in the inner layer gradually contacts the plasma collecting surface 3002 of the plasma collecting sleeve, and overflows upward from the plasma outlet slit 3004 along the plasma collecting body 3003.

[0051] 5) The plasma overflowing into the arc-shaped surface of the plasma collecting sleeve 3000 is centrifuged again. As the amount of plasma increases, the plasma overflows along the lower opening of the protective cover 1000 and the collected plasma is collected.

[0052] Specifically, plasma that overflows into the upper plasma collecting concave surface 3001 of the plasma collecting sleeve will form a centrifugal separation area on the concave surface, centrifuging the plasma again and improving the quality of the plasma. As the plasma in the upper plasma collecting concave surface 3001 increases, the plasma will overflow from the gap between the core shaft skirt 4002 and the core shaft upper skirt 5001, overflowing along the lower outlet of the protective cover, and the collected plasma will be collected.

[0053] 6) As the plasma is collected, the red blood cells will gradually move to the middle until they reach the overflow outlet of the protective cover 1000 and the separation stops.

[0054] As plasma is collected, red blood cells will gradually move toward the middle until they reach the overflow outlet of the protective cover 1000. Once the device detects this, the separation will stop and the red blood cells will be continuously delivered to the plasma donor for the next collection and separation until the required amount is reached.

[0055] like Figure 9 As shown, the reduced collection surface 4002 of the plasma collection sleeve in the present invention increases the blood volume entering the separation cup at a single time, and more plasma components are separated at a single time, thereby reducing the required time for the total amount;

[0056] The shortened plasma collecting sleeve and plasma collecting body 3003 can reduce friction when a single cycle stops and when the collected plasma contacts the plasma collecting surface 3002, thereby reducing the generation of plasma foam and improving the quality of plasma.

[0057] The reduced core shaft skirt 4002 and the core shaft upper skirt 5001 prolong the plasma separation time and improve the plasma quality.

[0058] The design of the upper plasma collecting concave surface 3001 enables a second brief separation of the plasma, thereby improving the quality of the plasma.

[0059] The added slurry discharge slit 3004 of the slurry collecting sleeve and the gap between the core shaft seat 4000 and the core shaft upper seat 5000 make the slurry discharge smoother, improve the plasma quality and reduce the slurry discharge time.

[0060] It is obvious to those skilled in the art that the above-mentioned specific examples are only preferred embodiments of the present invention. Therefore, any improvements and changes that may be made by those skilled in the art to certain parts of the present invention still reflect the principles of the present invention and achieve the purpose of the present invention, and all fall within the scope of protection of the present invention.

Claims

1. A centrifugal plasma separation cup, characterized in that: include: A cup body (2000) is provided, wherein an arc-shaped slurry collecting sleeve (3000) is installed at the entrance of the cup body (2000); a pressure cover (6000) for fixing the slurry collecting sleeve (3000) is provided on the mouth of the cup body (2000); a core shaft tube (7000) is installed in the cup body (2000); the core shaft tube (7000) is provided with an upper and a lower opening after passing through the slurry collecting sleeve (3000) and the pressure cover (6000); a sealing member for sealing the core shaft tube (7000) is provided on the pressure cover (6000); and a protective cover (1000) is provided on the sealing member.

2. The centrifugal plasma separation cup according to claim 1, characterized in that: The slurry collecting sleeve (3000) comprises: a slurry collecting body (3003); a slurry outlet seam (3004) is provided on the upper edge of the slurry collecting body (3003); an upper slurry collecting concave surface (3001) is provided inside the slurry collecting body (3003); and a slurry collecting surface (3002) is provided on the outer surface of the slurry collecting body (3003).

3. The centrifugal plasma separation cup according to claim 2, characterized in that: The upper slurry collecting concave surface (3001) is a concave structure, and the angle between the upper slurry collecting concave surface (3001) and the inner wall of the slurry collecting body (3003) is in the range of 60 degrees to 70 degrees.

4. A centrifugal plasma separation cup according to claim 1 or 2, characterized in that: A core shaft seat (4000) is installed between the pressure cover (6000) and the fixed slurry collecting sleeve (3000), and the upper end of the core shaft seat (4000) is provided with an upper opening and a lower opening after passing through the slurry collecting sleeve (3000) and the pressure cover (6000). The core shaft tube (7000) is installed at the lower end of the core shaft seat (4000), and the core shaft upper seat (5000) is mounted on the core shaft seat (4000).

5. The centrifugal plasma separation cup according to claim 4, characterized in that: The spindle seat (4000) comprises: a spindle seat skirt (4002), a spindle seat skirt surface (4003) is installed on the spindle seat skirt (4002), a spindle seat three-point upper plane (4001) is provided on the spindle seat skirt surface (4003), an output tube (4004) is clamped on the spindle seat three-point upper plane (4001), and the upper and lower ports are provided on the output tube (4004).

6. The centrifugal plasma separation cup according to claim 5, characterized in that: The plane (4001) on the three points of the core shaft seat is three evenly arranged support ribs.

7. The centrifugal plasma separation cup according to claim 6, characterized in that: The core shaft upper seat (5000) comprises: a core shaft upper seat skirt (5001) and a core shaft upper seat lower plane (5002), and the core shaft upper seat lower plane (5002) is sleeved on the core shaft seat skirt surface (4003).

8. The centrifugal plasma separation cup according to claim 7, characterized in that: The core shaft skirt (4002) and the core shaft upper skirt (5001) form a smooth pulping gap, and the three supporting ribs on the three-point upper plane (4004) of the core shaft seat enable a pulping gap to be formed between the core shaft seat (4000) and the core shaft upper seat (5000).

9. The centrifugal plasma separation cup according to claim 1 or 2, characterized in that: The sealing component comprises: a graphite ring (8000), a rubber bowl (9000), and a ceramic ring (A000) which are sequentially installed on the gland (6000) from bottom to top.

10. A separation method based on the centrifugal plasma separation cup according to claim 1, characterized in that: The following steps are involved: 1) Whole blood containing anticoagulant is input from the upper port and enters the cup body (2000) through the core shaft tube (7000); 2) The cup body (1000) is mounted on a plasma collection device and rotates at high speed; 3) The slurry collecting sleeve (3000), the gland (6000) and the sealing element are fixedly connected and then rotated rapidly to form a dynamic sealing system; 4) As the blood rotates at high speed in the cup body (2000), cells are separated, and red blood cells are thrown to the outer layer by centrifugal force, while the plasma with lighter cells is in the inner layer; wherein, the plasma in the inner layer gradually contacts the plasma collecting sleeve (3000) and overflows upward along the plasma collecting sleeve (3000); 5) The plasma overflowing into the arc-shaped surface of the plasma collecting sleeve (3000) is centrifuged again. The plasma is centrifuged again. As the amount of plasma increases, the plasma overflows along the lower opening of the protective cover (1000), and the collected plasma is collected. 6) As the plasma is collected, the red blood cells will gradually move to the middle until they reach the overflow outlet under the protective cover (1000) and the separation stops.

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