A bio-bacteria active material, a preparation method and application thereof

Abstract

This invention discloses a bioactive material, its preparation method, and its application. The raw materials of the bioactive material comprise the following components by mass percentage: 20-50% bioactive filler, 5%-20% oxygen-generating filler, 10-30% maifanite, 0-30% ceramic particles, 0-30% quartz sand, 5-20% aluminum phosphate cement, 1-3% water-reducing agent, 10-30% bioactive bacterial composition, and 3-5% plant gum. The bioactive material provided by this invention can effectively maintain a healthy aquatic ecosystem and can be widely applied in the field of water purification.

Description

Technical Field

[0001] This invention relates to the field of water treatment, and more particularly to the field of microbial water purification, specifically to a biological active material and its preparation method and application. Background Technology

[0002] Home aquariums require regular water changes because over time, ammonia and nitrate levels increase, blue-green algae grow on the glass, the water becomes cloudy, and harmful bacteria such as Aeromonas vera, Vibrio, and Streptococcus proliferate, leading to fish deaths and an unsightly appearance. Frequent water changes increase labor and costs, and also pollute the environment. Large aquariums face similar problems, requiring frequent water changes, often several tons each time (hundreds of tons in some cases), resulting in water waste and pollution of waterways. Therefore, it is essential to minimize water changes while ensuring the healthy survival of aquarium fish.

[0003] In addition, small rivers such as those in towns and cities often have foul odors due to poor water flow, and the water quality is often black, with blue-green algae. Furthermore, the organic matter and heavy metals in these rivers are seriously exceeding the standard (BOD5 > 10 mg / L). Therefore, an effective solution is needed to improve the water quality of these rivers and restore their ecology.

[0004] In existing technologies for water purification using biological clay blocks, most of the clay pores are closed pores, which prevent the survival of beneficial bacteria. Ordinary cement is highly alkaline, severely impacting the reproduction and survival of these bacteria. Furthermore, the large amount of bacteria required increases costs. Additionally, the proportion of pores for bacterial reproduction per cubic meter is only 10%-20%, resulting in poor biological purification effects. Summary of the Invention

[0005] The main objective of this invention is to provide a biological active material, its preparation method, and its application, so as to overcome the shortcomings of the prior art.

[0006] To achieve the aforementioned objectives, the technical solution adopted by this invention includes:

[0007] One aspect of the present invention provides a bioactive material, the raw materials of which include the following components calculated by weight percentage: 20-50% bioactive filler, 5%-20% oxygen-generating filler, 10-30% maifanite, 0-30% ceramic particles, 0-30% quartz sand, 5-20% aluminum phosphate cement, 1-3% water-reducing agent, 10-30% bioactive spore composition and 3-5% plant gum;

[0008] The raw materials of the bioactive filler include the following components calculated by mass percentage: 30-70% aluminum phosphate cement, 20-60% volcanic mud, 5-40% maifanite powder, 2-10% active additives, 0.5-2% agar, 2-5% wheat bran, 3-8% bagasse, 0.1-0.5% yeast extract, and 0.1-1% dihydrogen phosphate; the active additives include any one or a combination of two of far-infrared powder and silica fume.

[0009] Another aspect of the present invention provides a method for preparing the aforementioned bioactive material, comprising: mixing bioactive filler, oxygen-generating filler, maifanite, ceramic particles (optionally added or not added), quartz sand (optionally added or not added), aluminum phosphate cement, a bioactive spore composition, plant glue, a water-reducing agent, and water to obtain a mixture, and molding the mixture to obtain the bioactive material.

[0010] Another aspect of the present invention provides the application of the aforementioned bioactive materials in water purification.

[0011] Another aspect of the present invention provides a water purification method, which includes: immersing the aforementioned biological active material in the water body to be purified.

[0012] Compared with the prior art, the present invention has at least the following advantages:

[0013] The bioactive material provided by this invention can activate the spores of bacteria in water, transforming them into bioactive bacteria. The oxygen-generating filler provides oxygen for the bacteria. The bioactive bacteria enter the water and use harmful substances in the water as food (ammonia nitrogen, nitrates, phosphate, organic matter, etc.), which helps to prevent the formation of blue-green algae and reduce the growth of harmful bacteria. The bioactive bacteria reproduce and grow in the bio-block, which can effectively maintain a good aquatic ecosystem and can be widely used in the field of water purification. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 Photograph of the bioactive material prepared in Example 1 of this invention. Detailed Implementation

[0016] The invention will be more fully understood by reading the following detailed description. However, it should be understood that the detailed description disclosed below is merely exemplary of the invention, which can be embodied in various forms. Therefore, the specific functional details disclosed herein should not be construed as limiting, but rather as the basis for the claims and as a representative basis for teaching those skilled in the art to employ the invention in different ways in any suitable detailed embodiment.

[0017] As one aspect of the technical solution of the present invention, the raw materials of a bioactive material include the following components calculated by mass percentage: 20-50% bioactive filler, 5%-20% oxygen-generating filler, 10-30% maifanite, 0-30% ceramic particles, 0-30% quartz sand, 5-20% aluminum phosphate cement, 1-3% water-reducing agent, 10-30% bioactive spore composition, and 3-5% plant gum;

[0018] The raw materials of the bioactive filler include the following components calculated by mass percentage: 30-70% aluminum phosphate cement, 20-60% volcanic mud, 5-40% maifanite powder, 2-10% active additives, 0.5-2% agar, 2-5% wheat bran, 3-8% bagasse, 0.1-0.5% yeast extract, and 0.1-1% dihydrogen phosphate; the active additives include any one or a combination of two of far-infrared powder and silica fume.

[0019] In some embodiments, the bioactive filler is a spherical particle with a particle size of 0.4-0.6 cm.

[0020] In some embodiments, the preparation method of the bioactive filler includes: adding aluminum phosphate cement, volcanic mud, maifanite powder, active additives, agar, wheat bran, bagasse, yeast extract and dihydrogen phosphate into a round pot granulator for water spraying to form balls, thereby obtaining the bioactive filler (energy balls).

[0021] In some preferred embodiments, the particle size of the volcanic mud is 250-400 mesh.

[0022] In some preferred embodiments, the particle size of the active additive is 250-400 mesh.

[0023] In some preferred embodiments, the particle size of the maifanite powder in the raw material of the bioactive filler is 250-400 mesh.

[0024] In some preferred embodiments, the agar is in powder form with a particle size of 200 mesh to 320 mesh.

[0025] In some preferred embodiments, the yeast paste is in powder form with a particle size of 200-320 mesh.

[0026] In some preferred embodiments, the bran has a particle size of 200 mesh to 320 mesh.

[0027] In some preferred embodiments, the sugarcane bagasse has a particle size of 200 mesh to 320 mesh.

[0028] In some preferred embodiments, the process conditions for water spraying pelletizing include: the rotation speed of the round pot granulator is 10-35 r / min, the temperature for water spraying pelletizing is room temperature, the time for water spraying pelletizing is 20-30 min, and the amount of water sprayed is 18-35% of the dry material mass.

[0029] In this invention, bioactive filler (energy balls) provides energy and a framework; maifan stone is used to build the structure (biological space for reproduction) and provide metal ions; waste ceramic particles and quartz sand are used for building the structure; aluminum phosphate cement improves strength; water-reducing agent reduces the amount of water added; and plant glue can enhance the structural stability of the bioactive material within a certain period of time, and it can slowly degrade naturally. During this process, it can release some components that are beneficial to the growth of biological bacteria and promote the slow disintegration of the bioactive material, which is beneficial to environmental protection.

[0030] In this invention, aluminum phosphate cement is used to increase strength during pellet making and is neutral, causing no harm to microorganisms; volcanic mud provides activating energy for microorganisms, increasing their activity and improving pellet strength, while also releasing some Fe metal ions. 2+ Mn 2+ Zn 2+ Ions required for the reproduction of beneficial microorganisms; some metal ions, Fe, can be precipitated from maifanite powder. 2+ Mn 2+ Zn 2+ It contains ions needed for the reproduction of beneficial microorganisms; far-infrared powder is used to generate energy; silica fume can generate energy and release infrared waves. The far-infrared waves released by its own polarization characteristics can vibrate large water molecule clusters into smaller water molecule clusters, thereby activating the water. Activated water has good permeability and solubility, which plays a significant role in the metabolism of beneficial microorganisms.

[0031] In this invention, the plant fibers contained in sugarcane bagasse and wheat bran are combined with cement, volcanic mud, maifan stone powder, etc. On the one hand, the bioactive filler can have a loose and porous structure that can maintain a stable structure for a long time. On the other hand, it can slowly release components in the bioactive material that promote the growth of bio-bacterial spores into mycelia, thus promoting the purification function, while avoiding the excessive reproduction of bio-bacterial spores.

[0032] In some embodiments, the preparation method of the oxygen-generating filler includes: mixing zeolite powder, polymer gel and green algae evenly to obtain a mixture, dropping the mixture into a calcium chloride solution to form gel beads, and then solidifying it to obtain the oxygen-generating filler.

[0033] In some preferred embodiments, the polymeric gel includes sodium alginate and polyvinyl alcohol.

[0034] In some preferred embodiments, the preparation method of the oxygen-generating filler includes: adding 4-10 parts by weight of 0.5-1μm zeolite powder and 2-4 parts by weight of polymer gel (e.g., sodium alginate or polyvinyl alcohol) to 100 parts by weight, ultrasonically dispersing until uniform, and adding to a concentration of 10. 7 cells-10 8 30-60 parts of a green algae aqueous suspension with cells / mL were stirred evenly and then dropped into a calcium chloride solution with a concentration of 2wt%-4wt% to form gel beads of 2-4 mm. The mixture was then solidified for 30-50 minutes to obtain the oxygen-generating filler.

[0035] In some more specific embodiments, the preparation method of the oxygen-generating filler includes: adding 4-10 grams of 0.5-1 μm zeolite powder and 2-4 grams of sodium alginate or polyvinyl alcohol to 100 ml of water, ultrasonically dispersing evenly, and then adding a 10% concentration... 7 cells-10 8 30-60 ml of a green algae aqueous suspension (cells / mL) was stirred evenly and then dropped into a 2-4 wt% calcium chloride solution to form 2-4 mm gel beads. The solution was then solidified for 30-50 minutes to obtain the oxygen-generating filler.

[0036] In this invention, the oxygen-generating filler made from zeolite serves as a porous material, acting as an adsorbent to increase the specific surface area and provide mechanical strength. It adsorbs green algae cells onto the surface and within the pores, embedding them within a composite matrix of sodium alginate or polyvinyl alcohol. The green algae produce oxygen in the presence of light, providing sufficient oxygen for the beneficial microorganisms. At night, when the algae respire in the absence of light, the oxygen level in the water decreases slightly, but the small amount of algae does not affect fish growth or the beneficial microorganisms. Furthermore, lighting can be turned on at night to increase oxygen production.

[0037] In some embodiments, the particle size of maifanite in the raw materials of the bioactive material is 20-60 mesh.

[0038] In some embodiments, the ceramic particles have a particle size of 20-60 mesh.

[0039] In some embodiments, the quartz sand has a particle size of 20-60 mesh.

[0040] In some implementations, the water-reducing agent includes, but is not limited to, polycarboxylate water-reducing agents.

[0041] In some embodiments, the biological spore composition comprises 30-60 wt% Bacillus subtilis spores, 15-30 wt% Bacillus thuringiensis spores, 20-40 wt% Bacillus spheroidis spores, 5-15 wt% yeast and 5-25 wt% lactic acid bacteria.

[0042] In some embodiments, the bioactive material is a block material with a porosity of 30-45% and the pores having a diameter of 20μm-5mm.

[0043] As another aspect of the technical solution of the present invention, the preparation method of the aforementioned bioactive material includes: mixing bioactive filler, oxygen-generating filler, maifan stone, ceramic particles (optionally added or not added), quartz sand (optionally added or not added), aluminum phosphate cement, bioactive spore composition, plant glue, water-reducing agent and water to obtain a mixture, and molding the mixture to obtain the bioactive material.

[0044] In some embodiments, the water content in the mixture is 8-15% by mass.

[0045] In some embodiments, the molding process conditions include: placing the mixture in a mold and drying it at room temperature.

[0046] In some more specific embodiments, the preparation method of the bioactive material includes the following steps:

[0047] 1) Mix aluminum phosphate cement, volcanic mud, far-infrared powder, maifanite powder, silica fume, agar, wheat bran, bagasse, yeast extract and dihydrogen phosphate and add them to a round pot granulator to start water spraying to make balls. According to the ball making time, make the balls with a diameter of about 0.4cm-0.6cm and air dry them for later use to obtain the bioactive filler.

[0048] 2) Add 4-10 grams of 0.5-1μm zeolite powder and 2-4 grams of sodium alginate or polyvinyl alcohol to 100 ml of water, and ultrasonically disperse until uniform. Add water to a concentration of 10... 7 cells-10 8 30-60 ml of a green algae aqueous suspension (cells / mL) was stirred evenly and then dropped into a calcium chloride solution with a concentration of 2wt%-4wt% to form gel beads of 2-4 mm. The solution was then solidified for 30-50 min to obtain the oxygen-generating filler.

[0049] 3) Mix the prepared bioactive filler, oxygen-generating filler, maifan stone, ceramic particles, quartz sand, aluminum phosphate cement, plant glue, water-reducing agent, and biological spore composition. Add water and stir quickly. Pour the mixture into a 10cm×10cm×20cm plastic mold and dry it in a 40-50℃ drying oven. After demolding, the finished product is obtained, thus producing the bioactive material.

[0050] As another aspect of the technical solution of this invention, it relates to the application of the aforementioned biological active materials in water purification. Examples include water purification treatment of aquariums, large ornamental fish ponds, rivers, and polluted ditches.

[0051] As another aspect of the technical solution of the present invention, a water purification method includes: immersing the aforementioned biological active material in the water body to be purified.

[0052] The bioactive filler (energy balls) in the bioactive material (bioblock) provided by this invention are connected by aluminum phosphate cement to form a robust structure. The joints between the balls are interconnected. Quartz sand, ceramic particles, and maifanite accumulate at the joints to form numerous interconnected micron and millimeter pores, creating a habitat for the microorganisms. When the bioblock comes into contact with water, the energy balls emit far-infrared waves, converting surrounding aggregated water molecules into smaller water molecules and increasing dissolved oxygen levels. This stimulates the microorganisms to spore and transform into active bacteria that use harmful substances in the water as food, thus clarifying the water quality. Volcanic mud and maifanite precipitate Fe... 2+ Mg 2+ Ca 2 + Mn 2+ The nutrients are provided to the microorganisms, creating a suitable environment for their reproduction. The oxygen-generating filler, through the photosynthesis of green algae, produces oxygen, which is then supplied to the microorganisms.

[0053] The present invention is further illustrated below by way of examples, but the invention is not limited to the scope of the examples described. All reagents and raw materials used in the following examples are commercially available, and test methods not specifically specified are generally performed under conventional conditions or according to the conditions recommended by the respective manufacturers. For example, the silica fume used in the following examples is furnace ash from a steel plant, whose mineral composition is mainly monocrystalline silicon, emitting long wavelengths of 3µm-1mm.

[0054] Example 1

[0055] A method for preparing a bioactive bacterial material includes the following steps:

[0056] Preparation of bioactive filler (energy balls): 40 kg of aluminum phosphate cement, 30 kg of volcanic mud, 25 kg of maifanite powder, 5 kg of far-infrared powder, 1.5 kg of agar, 3.5 kg of wheat bran, 4 kg of sugarcane bagasse, 0.5 kg of yeast extract, and 0.5 kg of dihydrogen phosphate were added to a round pot granulator. The mixture was stirred at 15 r / min for 20 minutes in the round pot granulator, and water was sprayed at room temperature. The amount of water sprayed was about 18% of the dry material. When the diameter of the balls reached 0.5 cm, the balls were sieved and the balls with a diameter of 0.4 cm to 0.6 cm were retained. The smaller balls were returned to the round pot granulator to obtain the bioactive filler, which was then dried.

[0057] Preparation of oxygen-generating packing material: Add 6 grams of 0.5-1μm zeolite powder and 2 grams of sodium alginate to 100 ml of water, and ultrasonically disperse until uniform. Add the packing material at a concentration of 10... 7 40 ml of a chlorophyll / mL aqueous suspension of green algae was stirred evenly and then dropped into a 2 wt% calcium chloride solution to form 2-4 mm gel beads. After solidification for 30 min, the oxygen-generating filler was obtained.

[0058] Preparation of bioactive materials: 36 kg of energy balls, 8 kg of oxygen-generating filler, 18 kg of maifanite granules, 25 kg of quartz sand, 2 kg of polycarboxylate superplasticizer, 18 kg of aluminum phosphate cement, and 18 kg of a mixture of bioactive spores (containing 50% Bacillus subtilis, 15% Bacillus thuringiensis, 20% Bacillus spores, 8% yeast, and 7% lactic acid bacteria), 5 kg of guar gum, and 15 kg of water were mixed and molded in a 10cm×10cm×20cm mold. The mixture was then dried at approximately 50℃ to obtain the bioactive material (bioblock), as shown in the photograph. Figure 1 As shown.

[0059] Example 2

[0060] A method for preparing a bioactive bacterial material includes the following steps:

[0061] Preparation of bioactive filler (energy balls): 48 kg of aluminum phosphate cement, 30 kg of volcanic mud, 18 kg of maifanite powder, 4 kg of silica fume, 1 kg of agar, 4 kg of wheat bran, 4.4 kg of sugarcane bagasse, 0.3 kg of yeast extract, and 0.3 kg of dihydrogen phosphate were added to a round pot granulator. The mixture was stirred at 25 r / min for 25 minutes in the round pot granulator, and water was sprayed at room temperature. The amount of water sprayed was about 20% of the dry material. When the diameter of the balls reached 0.5 cm, the balls were sieved and the balls with a diameter of 0.4 cm to 0.6 cm were retained. The smaller balls were returned to the round pot granulator to obtain the bioactive filler, which was then dried.

[0062] Preparation of oxygen-generating packing material: Add 8 grams of 0.5-1μm zeolite powder and 4 grams of polyvinyl alcohol to 100 ml of water, disperse evenly with ultrasonic cleaning, and add a 10% concentration. 750 ml of a chlorophyll / mL aqueous suspension of green algae was stirred evenly and then dropped into a 2 wt% calcium chloride solution to form 2-4 mm gel beads. After curing for 40 min, the oxygen-generating filler was obtained.

[0063] Preparation of bioactive materials: 30 kg of energy balls, 10 kg of oxygen-generating filler, 25 kg of maifanite, 20 kg of quartz sand, 2 kg of polycarboxylate superplasticizer, 15 kg of aluminum phosphate cement, 23 kg of bioactive spore mixture (containing 55% Bacillus subtilis, 15% Bacillus thuringiensis, 20% Bacillus spheroids, 5% yeast, and 5% lactic acid bacteria), 5 kg of guar gum, and 15 kg of water were mixed and shaped in a 10cm×10cm×20cm mold. The mixture was then dried at approximately 40℃ to obtain bioactive materials (bioblocks).

[0064] Example 3

[0065] A method for preparing a bioactive bacterial material includes the following steps:

[0066] Preparation of bioactive filler (energy balls): 58 kg of aluminum phosphate cement, 24 kg of volcanic mud, 14 kg of maifanite powder, 4 kg of far-infrared powder, 1 kg of agar, 3 kg of wheat bran, 5 kg of sugarcane bagasse, 0.4 kg of yeast extract, and 0.6 kg of dihydrogen phosphate were added to a round pot granulator. The mixture was stirred for 20 minutes at a speed of 25 r / min in the round pot granulator, and water was sprayed at room temperature. The amount of water sprayed was about 25% of the dry material. When the diameter of the balls reached 0.5 cm, the balls were sieved and the balls with a diameter of 0.4 cm to 0.6 cm were retained. The smaller balls were returned to the round pot granulator to obtain the bioactive filler, which was then dried.

[0067] Preparation of oxygen-generating packing material: Add 6 grams of 0.5-1μm zeolite powder and 2 grams of polyvinyl alcohol to 100 ml of water, disperse evenly with ultrasonic cleaning, and add a 10% concentration... 7 40 ml of a chlorophyll / mL aqueous suspension of green algae was stirred evenly and then dropped into a 2 wt% calcium chloride solution to form 2-4 mm gel beads. After solidification for 30 min, the oxygen-generating filler was obtained.

[0068] Preparation of bioactive materials: 40 kg of energy balls, 15 kg of oxygen-generating filler, 25 kg of maifanite, 10 kg of quartz sand, 2 kg of polycarboxylate superplasticizer, 13 kg of aluminum phosphate cement, 20 kg of bioactive spore mixture (containing 45% Bacillus subtilis, 20% Bacillus thuringiensis, 15% Bacillus spheroidis, 10% yeast, and 10% lactic acid bacteria), 5 kg of soybean gum, and 13 kg of water were mixed and shaped in a 10cm×10cm×20cm mold. The mixture was then dried at approximately 50℃ to obtain bioactive materials (bioblocks).

[0069] Example 4

[0070] A method for preparing a bioactive bacterial material includes the following steps:

[0071] Preparation of bioactive filler (energy balls): 45 kg of aluminum phosphate cement, 25 kg of volcanic mud, 20 kg of maifanite powder, 10 kg of far-infrared powder, 0.5 kg of agar, 3 kg of wheat bran, 5.5 kg of sugarcane bagasse, 0.4 kg of yeast extract, and 0.6 kg of dihydrogen phosphate were added to a round pot granulator. The mixture was stirred at 30 r / min for 20 minutes in the round pot granulator, and water was sprayed at room temperature. The amount of water sprayed was about 18% of the dry material. When the diameter of the balls reached 0.5 cm, the balls were sieved to leave balls with a diameter of 0.4 cm to 0.6 cm. The smaller balls were returned to the round pot granulator to obtain the bioactive filler, which was then dried.

[0072] Preparation of oxygen-generating packing material: Add 10 grams of 0.5-1μm zeolite powder and 4 grams of sodium alginate to 100 ml of water, disperse evenly with ultrasonication, and add a 10% concentration... 8 45 ml of a chlorophyll / mL aqueous suspension of green algae was stirred evenly and then dropped into a 3 wt% calcium chloride solution to form 2-4 mm gel beads. After curing for 50 min, the oxygen-generating filler was obtained.

[0073] Preparation of bioactive materials: 50 kg of energy balls, 13 kg of oxygen-generating filler, 20 kg of maifanite, 12 kg of ceramic particles, 2 kg of polycarboxylate superplasticizer, 13 kg of aluminum phosphate cement, 16 kg of a mixture of biological spores (containing 52% Bacillus subtilis, 20% Bacillus thuringiensis, 10% Bacillus spheroides, 10% yeast, and 8% lactic acid bacteria), 4 kg of guar gum, and 12 kg of water were mixed and shaped in a 10 cm × 10 cm × 20 cm mold. The mixture was then dried at approximately 50 °C to obtain bioactive materials (bioblocks).

[0074] Example 5

[0075] A method for preparing a bioactive bacterial material includes the following steps:

[0076] Preparation of bioactive filler (energy balls): 35 kg of aluminum phosphate cement, 40 kg of volcanic mud, 15 kg of maifanite powder, 10 kg of silica fume, 1 kg of agar, 5 kg of wheat bran, 4 kg of sugarcane bagasse, 0.4 kg of yeast extract, and 0.6 kg of dihydrogen phosphate were added to a round pot granulator. The mixture was stirred at 25 r / min for 20 minutes in the round pot granulator, and water was sprayed at room temperature. The amount of water sprayed was about 22% of the dry material. When the diameter of the balls reached 0.5 cm, the balls were sieved and the balls with a diameter of 0.4 cm to 0.6 cm were retained. The smaller balls were returned to the round pot granulator to obtain the bioactive filler, which was then dried.

[0077] Preparation of oxygen-generating packing material: Add 10 grams of 0.5-1μm zeolite powder and 3 grams of sodium alginate to 100 ml of water, disperse evenly with ultrasonic cleaning, and add a 10% concentration... 7 50 mL of a chlorophyll / mL aqueous suspension of green algae was stirred evenly and then dropped into a 2 wt% calcium chloride solution to form 2-4 mm gel beads. After curing for 30 min, the oxygen-generating filler was obtained.

[0078] Preparation of bioactive materials: 45 kg of energy balls, 10 kg of oxygen-generating filler, 25 kg of maifanite, 8 kg of quartz sand, 2 kg of polycarboxylate superplasticizer, 10 kg of aluminum phosphate cement, 25 kg of a mixture of bioactive spores (containing 45% Bacillus subtilis, 10% Bacillus thuringiensis, 20% Bacillus spores, 10% yeast, and 15% lactic acid bacteria), 5 kg of guar gum, and 13 kg of water were mixed and shaped in a 10cm×10cm×20cm mold. The mixture was then dried at approximately 50℃ to obtain bioactive materials (bioblocks).

[0079] Comparative Example 1

[0080] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that the amount of far-infrared powder added during the preparation of the energy ball is 15 kg.

[0081] Comparative Example 2

[0082] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that the amount of bioactive spore mixture added in the raw materials for preparing the bioactive material is 1 kg.

[0083] Comparative Example 3

[0084] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that energy balls are not added to the raw materials for preparing the bioactive material.

[0085] Comparative Example 4

[0086] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that the energy balls in the raw materials for preparing the bioactive material are replaced with far-infrared powder of the same mass.

[0087] Comparative Example 5

[0088] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that far-infrared powder is omitted in the preparation of energy balls.

[0089] Comparative Example 6

[0090] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that the oxygen-generating filler is omitted.

[0091] Comparative Example 7

[0092] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that no plant gum is added during the preparation of the energy ball.

[0093] Comparative Example 8

[0094] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that agar is not added during the preparation of the energy ball.

[0095] Comparative Example 9

[0096] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that no bran is added during the preparation of the energy ball.

[0097] Comparative Example 10

[0098] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that sugarcane bagasse is not added during the preparation of the energy ball.

[0099] Comparative Example 11

[0100] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that yeast extract was not added during the preparation of the energy ball.

[0101] Comparative Example 12

[0102] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that dihydrogen phosphate is not added during the preparation of the energy ball.

[0103] Comparative Example 13

[0104] The preparation method of the bioactive material provided in this comparative example is basically the same as that in Example 1, except that agar, wheat bran, sugarcane bagasse, yeast extract and dihydrogen phosphate are omitted in the preparation of the energy ball.

[0105] Performance comparison test

[0106] Aquarium water purification test:

[0107] Water purification tests were conducted on Examples 1-5 and Comparative Examples 1-13. Twenty identical ornamental goldfish were placed in four one-cubic-meter aquariums, each containing three biological blocks prepared in the examples. One aquarium served as a blank control. Water quality was tested in January, March, and June, with the aquariums maintaining a constant water circulation system (air pump) to ensure a flowing water environment. Dissolved oxygen levels were measured at 5:00 AM. The test results are shown in Tables 1 to 7.

[0108] Because the goldfish are fed with biological feed from Anhui Jiguang Golden Dolphin, their feces float on top and can be cleaned regularly, or an automatic sewage system can be installed to clean them up.

[0109] Fish activity levels are categorized as good, average, poor, and dead. Goldfish aquarium water quality requirements include: BOD5 mg / L ≤ 5 mg / L, ammonia nitrogen ppm ≤ 0.02 ppm, nitrite ppm < 0.2 ppm, sodium ions ppm < 50 ppm (ideal value < 20 ppm), DO mg / l > 5 mg / l, and pH 7.0-8.0.

[0110] Table 1:

[0111]

[0112] Table 2:

[0113]

[0114] Table 3:

[0115]

[0116] Table 4:

[0117]

[0118]

[0119] Table 5:

[0120]

[0121] Table 6:

[0122]

[0123] Table 7:

[0124]

[0125] The data from Tables 1 to 7 show that in Comparative Example 1, the addition of far-infrared powder exceeding the standard in the energy balls had little impact on water quality. Because far-infrared powder is expensive, it was added within the prescribed range without affecting performance. In Comparative Example 2, the amount of added biological bacteria was relatively small, and water quality problems began to appear in March and June. In Comparative Example 3, no energy balls were added, and the porosity, as measured, was only 8.3%. The living environment for the biological bacteria was poor, with limited space, leading to water quality deterioration.

[0126] In Comparative Example 4, all energy balls were replaced with far-infrared powder, and the porosity of the bio-block was measured to be only 7.8%, resulting in limited living space for the microorganisms and deterioration of water quality. In Comparative Example 5, no far-infrared powder was used, and the water quality began to deteriorate over a longer period. In Comparative Example 6, no oxygen-generating filler was added, leading to a reduction in the proliferation of microorganisms and a certain impact on water quality, making it worse than the Example 1.

[0127] The test data in Tables 5 to 7 show that the concentrations of dissolved oxygen, BOD5, and sodium carbonate in the water of Comparative Examples 7-13 were higher than those of Examples 1-5 in the 6th month, indicating that the bioblocks lacking these components had a poor long-term purification effect on aquarium water. This may be because the bioblocks with these components have better stability. Plant gum can enhance the structural stability of the bioactive material for a certain period of time, and it can slowly degrade naturally. In this process, it can release some components that are beneficial to the growth of bacteria and promote the slow disintegration of the bioactive material, which is beneficial to the environment. Agar can absorb bacteria, and some bacteria can enter the agar, playing a role in stabilizing the bacteria. When there are few bacteria outside, the bacteria in the agar diffuse into the water, which is beneficial to the long-term stability of beneficial bacteria in the water. Wheat bran provides nutrients for bacteria, providing protein, vitamins, and minerals (magnesium, zinc), etc. The fiber of wheat bran forms a network structure that can adsorb bacteria, playing a role in stabilizing bacteria and providing carbon and nitrogen sources, which plays a role in the long-term stability of bacteria. Sugarcane bagasse provides a carbon and nitrogen source for microorganisms, and its fiber-forming network structure can adsorb microbial cells, thus stabilizing the microorganisms. Yeast extract provides comprehensive nutrition for microorganisms, supporting their growth. Dihydrogen phosphate regulates the pH stability of water, provides essential elements for microbial growth, and offers absorbable inorganic phosphorus.

[0128] Table 7 shows that the activity of fish in Comparative Example 13 began to decline in the sixth month. This is because Comparative Example 13 omitted agar, wheat bran, bagasse, yeast extract, and dihydrogen phosphate. The plant fibers in bagasse and wheat bran, combined with cement, volcanic mud, and maifanite powder, can, on the one hand, give the bioactive filler a loose and porous structure that can maintain stability over a longer period. On the other hand, it can slowly release components within the bioactive material that promote the growth of bacterial spores into mycelia, thus enhancing the purification function while preventing excessive spore proliferation. Furthermore, as mentioned earlier, agar, yeast extract, and dihydrogen phosphate are effective in stabilizing the microorganisms; therefore, the absence of these components reduces the long-term stability of the bio-block.

[0129] In addition, tests showed that the number of reproductive pores of the biological blocks prepared in the embodiments of the present invention reached more than 30%, which is more conducive to the growth and reproduction of biological bacteria in water. Furthermore, the blocks have high flexural strength and a certain degree of stability in water.

[0130] Pond water quality treatment test:

[0131] Before treatment: The pond smelled bad, there were no fish, and the bottom of the pond turned black and foamy.

[0132] Treatment Results: The biomass blocks prepared using the above method were introduced into the pond. After half a month, the pond water gradually became cleaner. BOD5 levels continued to decrease. After one month, ornamental fish were introduced, and their activity levels returned to normal.

[0133] In summary, through experimental testing, the bioactive material provided by this invention has the following advantages for water purification: 1) The aquatic ecosystem of aquariums is well maintained and can be maintained for a long time without water changes. The bio-blocks can also be placed in ornamental fish ponds to purify the water in large ornamental fish ponds; 2) Inorganic natural pigments can be added to the mixture to create artificial mountain colors, and plastic molds can be used to make artificial mountains, which increases the ornamental value and can also regulate water quality; 3) For polluted rivers and stinking ditches, the addition of bio-blocks can continuously reduce BOD5, clarify the water quality, and gradually restore the ecosystem.

[0134] In addition, the inventors of this case also conducted experiments with other raw materials, process operations, and process conditions described in this specification, referring to the aforementioned embodiments, and obtained relatively ideal results in all cases.

[0135] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A bioactive material, characterized in that, The raw materials of the bioactive material include the following components calculated by mass percentage: 20-50% bioactive filler, 5%-20% oxygen-generating filler, 10-30% maifanite, 0-30% ceramic particles, 0-30% quartz sand, 5-20% aluminum phosphate cement, 1-3% water-reducing agent, 10-30% bioactive spore composition, and 3-5% plant gum; The raw materials for the bioactive filler include the following components by mass percentage: 30-70% aluminum phosphate cement, 20-60% volcanic mud, 5-40% maifanite powder, 2-10% active additive, 0.5-2% agar, 2-5% wheat bran, 3-8% bagasse, 0.1-0.5% yeast extract, and 0.1-1% dihydrogen phosphate. The active additive includes any one or a combination of two of far-infrared powder and silica fume. The preparation method of the bioactive filler includes: adding aluminum phosphate cement, volcanic mud, maifanite powder, active additive, agar, wheat bran, bagasse, yeast extract, and dihydrogen phosphate into a round pot granulator for water spraying to form pellets, thereby obtaining the bioactive filler. The process conditions for water spraying to form pellets include: the rotation speed of the round pot granulator is 10-35 r / min, the water spraying temperature is room temperature, the water spraying time is 20-30 min, and the water spraying volume is 18-35% of the dry material mass. The preparation method of the oxygen-generating filler includes: mixing zeolite powder, sodium alginate and green algae suspension evenly to obtain a mixed solution, dropping the mixed solution into calcium chloride solution to form gel beads, and then solidifying it to obtain the oxygen-generating filler. The biological spore composition comprises 30-60 wt% Bacillus subtilis spores, 15-30 wt% Bacillus thuringiensis spores, 20-40 wt% Bacillus spheroidis spores, 5-15 wt% yeast and 5-25 wt% lactic acid bacteria.

2. The bioactive material according to claim 1, characterized in that, The bioactive filler is a spherical particle with a particle size of 0.4cm to 0.6cm.

3. The bioactive material according to claim 1, characterized in that, The particle size of the volcanic mud is 250-400 mesh.

4. The bioactive material according to claim 1, characterized in that, The particle size of the maifanite powder in the raw material of the bioactive filler is 250~400 mesh.

5. The bioactive material according to claim 1, characterized in that, The particle size of the active additive is 250-400 mesh.

6. The bioactive material according to claim 1, characterized in that, The agar has a particle size of 200-320 mesh.

7. The bioactive material according to claim 1, characterized in that, The particle size of the yeast extract is 200 mesh to 320 mesh.

8. The bioactive material according to claim 1, characterized in that, The bran has a particle size of 200-320 mesh.

9. The bioactive material according to claim 1, characterized in that, The sugarcane bagasse has a particle size of 200-320 mesh.

10. The bioactive material according to claim 1, characterized in that, The particle size of the maifanite in the raw material of the bioactive material is 20-60 mesh.

11. The bioactive material according to claim 1, characterized in that, The ceramic particles have a particle size of 20-60 mesh.

12. The bioactive material according to claim 1, characterized in that, The quartz sand has a particle size of 20-60 mesh.

13. The bioactive material according to claim 1, characterized in that, The water-reducing agent includes a polycarboxylate water-reducing agent.

14. The bioactive material according to claim 1, characterized in that, The plant gums include guar gum, guar gum, or coumarin gum.

15. The bioactive material according to claim 1, characterized in that, The bioactive material is a block material with a porosity of 30-45% and pores with a diameter of 20 mm. -5mm.

16. The method for preparing the bioactive material according to any one of claims 1-15, characterized in that, include: A mixture is prepared by mixing bioactive filler, oxygen-generating filler, maifan stone, optional ceramic particles, optional quartz sand, aluminum phosphate cement, biological spore composition, plant glue, water-reducing agent and water. The mixture is then subjected to molding treatment to obtain the bioactive material.

17. The preparation method according to claim 16, characterized in that, The water content in the mixture is 8-15% by mass.

18. The preparation method according to claim 16, characterized in that, The molding process conditions include: placing the mixture in a mold and drying it at room temperature.

19. The application of the bioactive material as described in any one of claims 1-15 in water purification.

20. A water purification method, characterized in that, include: The bioactive material described in any one of claims 1-15 is immersed in the water body to be purified.

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