Processing apparatus

Abstract

The invention relates to a processing device. Comprising a main machine, the main machine comprises a shell assembly, a piston and a liquid-isolating breathable film, the shell assembly is provided with a buffering cavity and a plurality of containing cavities which penetrate through the shell assembly in the axial direction of the main machine, a first buffering opening and a second buffering opening are formed in the two ends of the buffering cavity respectively, and a first containing opening and a second containing opening are formed in the two ends of each containing cavity respectively; the liquid-isolation breathable film is arranged on the shell assembly and seals and covers the second buffer storage opening and the second containing opening, and the piston is arranged in the buffer storage cavity in a sliding mode; and the exchange valve comprises a valve assembly rotationally connected with the shell assembly, the valve assembly is provided with an exchange channel, the exchange channel is provided with a first exchange port and a second exchange port which are arranged in a spaced mode, and when the valve assembly rotates, the first exchange port is always communicated with the first cache port, and the second exchange port is alternately communicated with different first containing ports. Therefore, cross contamination can be avoided, and working efficiency is improved.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a processing device. Background Art

[0002] Fully automated nucleic acid extraction is one of the core technologies supporting modern biomedicine. Through highly integrated and intelligent equipment, it enables the efficient and precise separation and purification of nucleic acids (DNA / RNA) from biological samples. Fully automated nucleic acid extraction utilizes a processing device that utilizes chemical and physical methods to integrate sample lysis, nucleic acid adsorption, washing, and elution steps. However, traditional processing devices often suffer from cross-contamination and low efficiency. Summary of the Invention

[0003] A technical problem solved by this application is how to avoid cross contamination and improve work efficiency.

[0004] A processing device, comprising:

[0005] A main unit, comprising a shell assembly, a piston, and a liquid-isolating and breathable membrane; the shell assembly is provided with a cache cavity and a plurality of accommodating cavities extending axially through the shell assembly; the two ends of the cache cavity respectively form a first cache opening and a second cache opening; the two ends of the accommodating cavity respectively form a first accommodating opening and a second accommodating opening; the liquid-isolating and breathable membrane is provided on the shell assembly and covers the second cache opening and the second accommodating opening; the piston is slidably provided in the cache cavity; and

[0006] An exchange valve includes a valve assembly rotatably connected to the shell assembly, the valve assembly is provided with an exchange channel, the exchange channel has a first exchange port and a second exchange port spaced apart, when the valve assembly rotates, the first exchange port is always connected to the first cache port, and the second exchange port is alternately connected to different first accommodating ports.

[0007] In one embodiment, the shell assembly includes a shell and a cover, the cover is connected to one end of the shell away from the exchange valve, the shell includes a bottom plate, an inner tube, an outer tube and a partition, the inner tube and the outer tube are both protruding from the bottom plate, the outer tube is arranged around the inner tube, the partition is connected to the inner tube and / or the inner tube, the space enclosed by the inner tube is configured as a part of the cache cavity, the space between the partitions is configured as a part of the accommodating cavity, and the second cache port and the second accommodating port are located on the cover.

[0008] In one embodiment, the cover body includes a first cover and a second cover, the first cover is fixedly connected to the shell, and the second cover is movably connected to the first cover to open or close the first cover, and a cache hole and a receiving hole are provided on the first cover and the second cover, the cache hole is configured as a part of the cache cavity and has the second cache port located in the second cover, and the receiving hole is configured as a part of the receiving cavity and has the second receiving port located in the second cover, and the liquid-isolating and breathable membrane is located between the first cover and the second cover and can block the cache hole and the receiving hole.

[0009] In one embodiment, the liquid-isolating and breathable membrane is fixedly connected to the second cover.

[0010] In one embodiment, the second cover includes a rotating portion and a connecting portion, the rotating portion is rotatably connected to the first cover, and the connecting portion is detachably connected to the first cover.

[0011] In one embodiment, the shell assembly further includes a sealing member connected to the shell body, the sealing member abuts between the valve assembly and the bottom plate, and the first cache port and the first accommodating port are located on the sealing member.

[0012] In one embodiment, the outer peripheral surface of the shell component includes a plurality of planes, the plurality of planes are arranged at intervals along the circumference of the shell component, and different planes correspond to different accommodating cavities.

[0013] In one embodiment, the valve assembly includes a driving member and a valve body, the driving member is provided with an exchange groove, the valve body is connected to the driving member and covers the opening of the exchange groove, and the valve body is provided with a first exchange hole and a second exchange hole connected to the exchange groove at intervals, the exchange groove, the first exchange hole and the second exchange hole are configured as the exchange channel, the first exchange port is located in the first exchange hole, and the second exchange port is located in the second exchange hole.

[0014] In one embodiment, the exchange valve further includes a carrier, the carrier is detachably connected to the shell assembly, the driving member abuts between the carrier and the valve body, and the valve body abuts against the shell assembly.

[0015] In one embodiment, at least one of the following options is also included:

[0016] The orthographic projections of the different first receiving ports all fall on the circumference formed by the rotation of the second exchange port;

[0017] The cache cavity has a contraction section arranged close to the first cache opening, and the diameter of the contraction section decreases from the second cache opening to the first cache opening.

[0018] A technical effect of an embodiment of the present application is: on the one hand, since the rotation of the valve assembly can make the cache chamber alternately connected with different accommodating chambers through the same exchange channel, the liquid is exchanged between the cache chamber and the different accommodating chambers, and finally the liquid in the different accommodating chambers is exchanged through the cache chamber, avoiding the use of multiple pipes to exchange the liquid, thereby simplifying the structure of the processing device, reducing the manufacturing cost of the processing device and improving the reliability. On the other hand, through the action of the liquid-isolating breathable membrane, the aerosol and liquid in the accommodating chamber are prevented from leaking out of the processing device, and the intrusion of external impurities into the cache chamber or the accommodating chamber can also be avoided, thereby avoiding cross contamination. On the other hand, the liquid is transferred within the same processing device, avoiding the transfer of the liquid between different devices, thereby reducing the transfer distance and time of the liquid, and thus improving the working efficiency of the processing device. At the same time, the transfer of the liquid can be automated by controlling the movement of the exchange valve and the piston, avoiding manual intervention, thereby further improving the working efficiency of the processing device. It can be understood that since manual intervention can be avoided in the process of liquid transfer, cross contamination between the liquid and the human body can also be avoided. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic diagram of the three-dimensional structure of a processing device provided in one embodiment.

[0020] Figure 2 for Figure 1 Schematic diagram of the exploded structure of the processing device shown.

[0021] Figure 3 for Figure 1 Schematic diagram of the partial decomposition structure of the processing device shown.

[0022] Figure 4 for Figure 1 The shown diagram is a three-dimensional structural diagram of the processing device when the second cover is in the open state.

[0023] Figure 5 for Figure 4 Schematic diagram of the decomposition structure.

[0024] Figure 6 for Figure 1 The shown schematic diagram is a three-dimensional cross-sectional structure of the processing device in an exploded state.

[0025] Figure 7 for Figure 1 The diagram shows a partial planar structure of the processing device after removing the exchange valve.

[0026] Figure 8 for Figure 1 Schematic diagram of the exploded structure of the valve assembly in the processing device shown.

[0027] Figure 9 for Figure 8 Schematic diagram of the planar structure of the driving part in the valve assembly shown.

[0028] Figure 10 for Figure 8 Schematic diagram of the planar structure of the valve body in the valve assembly shown.

[0029] Figure 11 for Figure 1 The shown diagram is a partial three-dimensional cross-sectional structural diagram of the processing device with the second cover removed.

[0030] 118 , a first portion of the housing 118 is provided, and a second portion of the housing 118 is provided. 86, sealing member 119, piston 120, liquid-isolating and breathable membrane 130, exchange valve 200, valve assembly 210, exchange channel 211, first exchange port 2111, second exchange port 2112, pressurizing port 2113, exchange tank 2114, first exchange hole 2115, second exchange hole 2116, transfer channel 212, first transfer port 2121, second transfer port 2122, transfer tank 2123, first transfer hole 2124, second transfer hole 2125, driving member 213, valve body 214, supporting member 220, reaction mechanism 300, reaction chamber 330, first reaction channel 310, second reaction channel 320, reaction plate 340, covering film 350, connecting member 360, collecting tube 400. DETAILED DESCRIPTION

[0031] To make the above-mentioned objects, features, and advantages of the present application more clearly understood, the specific embodiments of the present application are described in detail below with reference to the accompanying drawings. The following description sets forth many specific details to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways than those described herein, and those skilled in the art can make similar improvements without violating the scope of the present application. Therefore, the present application is not limited to the specific embodiments disclosed below.

[0032] In the description of this application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0033] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include at least one of such features. In the description of this application, if the term "plurality" appears, "plurality" means at least two, for example, two, three, etc., unless otherwise specifically defined.

[0034] In this application, unless otherwise specified or limited, the terms "mounted," "connected," "connected," "fixed," etc., should be interpreted broadly. For example, these terms may refer to fixed connections, removable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediary; and internal communication between two components or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.

[0035] In this application, unless otherwise expressly specified or limited, if a first feature is described as being "above" or "below" a second feature, or similar descriptions, this may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Furthermore, when a first feature is described as being "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is described as being "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0036] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only embodiment.

[0037] See Figure 1 、 Figure 2 、 Figure 3 and Figure 6 In one embodiment of the present application, a processing device 10 is provided for processing nucleic acids. The processing device 10 includes a main unit 100 and an exchange valve 200. The main unit 100 includes a shell assembly 110, a piston 120, and a liquid-isolating and breathable membrane 130. The shell assembly 110 is provided with a cache chamber 111 and a receiving chamber 112. There is one cache chamber 111 and multiple receiving chambers 112. The cache chamber 111 can be located at the center of the shell assembly 110, and multiple receiving chambers 112 are arranged at intervals around the cache chamber 111. The cache chamber 111 and the receiving chamber 112 penetrate the entire shell assembly 110 along the axial direction of the shell assembly 110, so that both the cache chamber 111 and the receiving chamber 112 have openings at the axial ends of the shell assembly 110. For example, the two ends of the buffer chamber 111 respectively form a first buffer opening 1111 and a second buffer opening 1112. The second buffer opening 1112 is further away from the exchange valve 200 than the first buffer opening 1111, so that the second buffer opening 1112 can be located above the first buffer opening 1111. The two ends of the accommodating chamber 112 respectively form a first accommodating opening 1121 and a second accommodating opening 1122. The second accommodating opening 1122 is further away from the exchange valve 200 than the first accommodating opening 1121, so that the second accommodating opening 1122 can be located above the first accommodating opening 1121. A liquid-barrier, breathable membrane 130 is disposed on the housing assembly 110. The liquid-barrier, breathable membrane 130 covers the second cache opening 1112 and the second receiving opening 1122. The liquid-barrier, breathable membrane 130 prevents liquid from passing through the liquid-barrier, breathable membrane 130 while allowing gas to pass through. This prevents liquid in the cache chamber 111 from leaking out of the second cache opening 1112 through the liquid-barrier, breathable membrane 130, and also prevents liquid in the receiving chamber 112 from leaking out of the second receiving opening 1122 through the liquid-barrier, breathable membrane 130. The piston 120 is slidably disposed within the cache chamber 111.

[0038] See Figure 2 、 Figure 3 、 Figure 7 and Figure 8The exchange valve 200 includes a valve assembly 210, which is rotatably connected to the shell assembly 110. For example, the valve assembly 210 can rotate about the central axis of the shell assembly 110. The valve assembly 210 defines an exchange channel 211. The exchange channel 211 has a first exchange port 2111 and a second exchange port 2112. The first exchange port 2111 and the second exchange port 2112 are spaced apart. When the valve assembly 210 rotates, the first exchange port 2111 is always connected to the first buffer port 1111, and the second exchange port 2112 is alternately connected to different first accommodating ports 1121.

[0039] When the piston 120 moves in the buffer chamber 111 near the exchange valve 200, that is, when the piston 120 moves downward, the pressure in the buffer chamber 111 can be increased, thereby allowing the liquid in the buffer chamber 111 to enter the exchange channel 211 through the first buffer port 1111 and the first exchange port 2111, and then enter the accommodating chamber 112 through the second exchange port 2112 and the first accommodating port 1121. Since the liquid-isolating and breathable membrane 130 allows gas to pass through, the gas in the accommodating chamber 112 can be discharged from the second accommodating port 1122 through the liquid-isolating and breathable membrane 130, making the air pressure in the accommodating chamber 112 equal to the external atmospheric pressure, ensuring that the liquid in the buffer chamber 111 can smoothly enter the accommodating chamber 112.

[0040] When the piston 120 moves away from the exchange valve 200 in the buffer chamber 111, that is, when the piston 120 moves upward, the pressure in the buffer chamber 111 can be reduced, allowing the liquid in the accommodating chamber 112 to enter the exchange channel 211 through the first accommodating port 1121 and the second exchange port 2112, and then enter the buffer chamber 111 through the first exchange port 2111 and the first buffer port 1111. Since the liquid-isolating and breathable membrane 130 allows gas to pass through, external gas can enter the accommodating chamber 112 through the liquid-isolating and breathable membrane 130, making the air pressure in the accommodating chamber 112 equal to the external atmospheric pressure, ensuring that the liquid in the accommodating chamber 112 can smoothly enter the buffer chamber 111.

[0041] See Figure 2 、 Figure 3 、 Figure 7 and Figure 8 Therefore, during the rotation of the valve assembly 210, when the second exchange port 2112 is in communication with the first receiving port 1121 of a different receiving chamber 112, the upward or downward movement of the piston 120 relative to the buffer chamber 111 can effectively achieve the transfer of liquid between the receiving chamber 112 and the buffer chamber 111. It can be understood that when the second exchange port 2112 is in communication with the first receiving port 1121 of one of the receiving chambers 112, the valve assembly 210 can block the first receiving port 1121 of the other receiving chamber 112, thereby preventing the liquid in the other receiving chamber 112 from flowing out of the first receiving port 1121.

[0042] See Figure 2 、 Figure 3 、 Figure 7 and Figure 8 In some embodiments, the first cache port 1111 and the first receiving port 1121 are both located above the exchange channel 211, allowing both the first cache port 1111 and the first receiving port 1121 to be located above the first exchange port 2111 and the second exchange port 2112. During the rotation of the valve assembly 210, the second exchange port 2112 may rotate about the center of the first exchange port 2111. The orthographic projections of the first receiving ports 1121 of different receiving chambers 112 along the axial direction of the shell assembly 110 may be located on the circumference formed by the rotation of the second exchange port 2112. This can also be understood as the first receiving ports 1121 being spaced apart along the same circumference of the shell assembly 110. Therefore, during the rotation of the valve assembly 210, when the second exchange port 2112 is located below the first receiving port 1121 of one of the receiving chambers 112 and is aligned with the first receiving port 1121, the exchange channel 211 can be communicated with the receiving chamber 112 through the second exchange port 2112, that is, the buffer chamber 111 is communicated with the receiving chamber 112 through the exchange channel 211. Conversely, when the second exchange port 2112 is misaligned with the first receiving port 1121 of the receiving chamber 112, the exchange channel 211 cannot be communicated with the receiving chamber 112 through the second exchange port 2112, and the buffer chamber 111 is communicated with the receiving chamber 112 through the exchange channel 211. Therefore, when the second exchange ports 2112 are alternately positioned below different first receiving ports 1121 and aligned with each other, the buffer chamber 111 can be alternately connected to different receiving ports 112 via the exchange channel 211, thereby enabling liquid to be transferred between different receiving ports 112 through the buffer chamber 111. It will be understood that when the second exchange port 2112 is aligned with one of the first receiving ports 1121, the other first receiving ports 1121 are misaligned with the second exchange port 2112 rather than aligned.

[0043] The accommodating chamber 112 can be used to hold substances such as samples or reagents. The multiple accommodating chambers 112 may include a sample chamber, a lysis liquid chamber, a first cleaning liquid chamber, a second cleaning liquid chamber, an elution liquid chamber, and an extraction chamber. The sample chamber is used to hold samples. Of course, the sample chamber liquid can be used to hold waste liquid generated during the processing process, so that the sample chamber can be used as a waste liquid chamber, that is, the sample chamber and the waste liquid chamber are shared. The lysis liquid chamber is used to hold the lysis liquid, the first cleaning liquid chamber is used to hold the first cleaning liquid, the second cleaning liquid chamber is used to hold the second cleaning liquid, and the elution liquid chamber is used to hold the elution liquid. The elution liquid can be enzyme-free water, etc. The extraction chamber can accommodate freeze-dried magnetic beads.

[0044] See Figure 2 、 Figure 3 、 Figure 7 and Figure 8In the process of extracting nucleic acid from a sample by the processing device 10, the following steps may be formed:

[0045] In the first step, various reagents are added into different containing chambers 112 respectively, and the sample to be processed is added into the sample chamber.

[0046] In the second step, the valve assembly 210 is rotated so that the second exchange port 2112 is positioned below the sample chamber. Specifically, the second exchange port 2112 is aligned with the first receiving port 1121 of the sample chamber. At this point, the piston 120 moves upward, drawing the sample from the sample chamber into the buffer chamber 111 through the exchange channel 211. The valve assembly 210 is then moved so that the second exchange port 2112 is positioned below the lysate chamber. The piston 120 moves upward, drawing the lysate from the lysate chamber into the buffer chamber 111, which contains the sample. At this point, a mixed solution of the lysate and sample forms in the buffer chamber 111. The valve assembly 210 is then moved so that the second exchange port 2112 is positioned below the extraction chamber. The piston 120 moves downward, injecting the mixed solution of the lysate and sample in the buffer chamber 111 into the extraction chamber through the exchange channel 211. Of course, the extraction chamber already contains freeze-dried magnetic beads.

[0047] In the third step, an external ultrasonic device is used to contact the position of the shell component 110 corresponding to the extraction chamber. Under the action of ultrasonic waves, the cells in the sample are materially broken by the lysis solution, thereby releasing the nucleic acids in the cells, thereby achieving nucleic acid lysis.

[0048] In the fourth step, the valve assembly 210 is rotated so that the second exchange port 2112 is located below the binding liquid chamber. The piston 120 moves upward, thereby sucking the binding liquid in the binding liquid chamber into the buffer chamber 111 through the exchange channel 211. Then, the valve assembly 210 is rotated so that the second exchange port 2112 is located below the extraction chamber. The piston 120 moves downward, thereby injecting the binding liquid in the buffer chamber 111 into the extraction chamber through the exchange channel 211.

[0049] In the fifth step, an external ultrasonic device is used to contact the position of the shell assembly 110 corresponding to the extraction chamber. Under the action of ultrasound, the nucleic acids in the liquid and the freeze-dried magnetic beads are thoroughly mixed and incubated, ensuring that all nucleic acids in the liquid are adsorbed to the freeze-dried magnetic beads, making the freeze-dried magnetic beads an attachment carrier for the nucleic acids. Obviously, when all nucleic acids are adsorbed to the freeze-dried magnetic beads, the liquid in the extraction chamber will no longer contain nucleic acids and will become waste liquid.

[0050] In the sixth step, an external magnet is adsorbed on the position of the shell assembly 110 corresponding to the extraction chamber. The magnetic attraction force generated by the external magnet adsorbs the freeze-dried magnetic beads adsorbed with nucleic acid to the inner wall surface of the extraction chamber, thereby preventing the freeze-dried magnetic beads adsorbed with nucleic acid from falling off from the inner wall surface.

[0051] The seventh step is to continue to adsorb the external magnet on the shell assembly 110 to ensure that the freeze-dried magnetic beads adsorbed with nucleic acid are adsorbed on the inner wall surface of the extraction chamber. In view of the fact that the second exchange port 2112 is still located below the extraction chamber, the piston 120 can be moved upward so that the waste liquid in the extraction chamber is sucked into the cache chamber 111 through the exchange channel 211. It can be understood that in the process of the waste liquid being sucked into the cache chamber 111, the freeze-dried magnetic beads are adsorbed on the inner wall surface of the extraction chamber, so that the freeze-dried magnetic beads adsorbed with nucleic acid cannot be sucked into the cache chamber 111, effectively avoiding the loss of freeze-dried magnetic beads. Then, by rotating the valve assembly 210, the second exchange port 2112 is located below the sample chamber, that is, below the waste liquid chamber, and the waste liquid in the cache chamber 111 can be injected into the sample chamber through the exchange channel 211 by moving downward the piston 120. At this time, there is no liquid in the extraction chamber, but only freeze-dried magnetic beads adsorbed with nucleic acid, thereby achieving the binding of nucleic acids.

[0052] In the eighth step, the valve assembly 210 is rotated so that the second exchange port 2112 is located below the first cleaning liquid chamber, and the piston 120 moves upward to suck the first cleaning liquid in the first cleaning liquid chamber into the cache chamber 111. Then the valve assembly 210 is rotated so that the second exchange port 2112 is located below the extraction chamber, and the piston 120 moves downward to inject the first cleaning liquid in the cache chamber 111 into the extraction chamber. Remove the external magnet, so that the freeze-dried magnetic beads adsorbed with nucleic acid will no longer be adsorbed to the inner wall of the extraction chamber and enter the first cleaning liquid. Then, the external ultrasonic device is used to contact the shell assembly 110 at the corresponding position of the extraction chamber, so that the freeze-dried magnetic beads adsorbed with nucleic acid and the first cleaning liquid are mixed under the action of ultrasonic waves, so that the first cleaning liquid cleans the freeze-dried magnetic beads, thereby further removing impurities on the freeze-dried magnetic beads. After cleaning is completed, the external magnet is adsorbed on the shell assembly 110 to ensure that the freeze-dried magnetic beads adsorbed with nucleic acid are re-adsorbed on the inner wall of the extraction chamber, and the piston 120 moves upward, thereby sucking the waste liquid generated in the extraction chamber after cleaning with the first cleaning liquid into the cache chamber 111. It can be understood that the freeze-dried magnetic beads adsorbed with nucleic acid are retained in the extraction chamber due to adsorption on the inner wall of the extraction chamber, thereby avoiding the loss of freeze-dried magnetic beads. The valve assembly 210 is then rotated so that the second exchange port 2112 is located below the sample chamber, causing the piston 120 to move downward and injecting the waste liquid in the cache chamber 111 into the sample chamber. At this time, there is no liquid in the extraction chamber, but only freeze-dried magnetic beads adsorbed with nucleic acid.

[0053] In step 9, step 8 can be repeated multiple times, so that the first cleaning solution can be used to clean the freeze-dried magnetic beads multiple times, thereby improving the cleaning effect of impurities. Of course, if step 8 only needs to be performed once, step 9 can be omitted, and the following step 10 operation can be directly performed.

[0054] The tenth step is to rotate the valve assembly 210 so that the second exchange port 2112 is located below the second cleaning liquid chamber, so that the piston 120 moves upward, and the second cleaning liquid in the second cleaning liquid chamber is sucked into the cache chamber 111. Then rotate the valve assembly 210 so that the second exchange port 2112 is located below the extraction chamber, so that the piston 120 moves downward, and the second cleaning liquid in the cache chamber 111 is injected into the extraction chamber. Remove the external magnet, so that the freeze-dried magnetic beads adsorbed with nucleic acid will no longer be adsorbed to the inner wall of the extraction chamber and enter the second cleaning liquid. Then, the external ultrasonic device is used to contact the shell assembly 110 at the corresponding position of the extraction chamber, so that the freeze-dried magnetic beads adsorbed with nucleic acid and the second cleaning liquid are mixed under the action of ultrasonic waves, so that the second cleaning liquid cleans the freeze-dried magnetic beads, thereby further removing impurities on the freeze-dried magnetic beads. After cleaning is completed, the external magnet is adsorbed on the shell assembly 110 to ensure that the freeze-dried magnetic beads adsorbed with nucleic acid are re-adsorbed on the inner wall of the extraction chamber, and the piston 120 moves upward, thereby sucking the waste liquid generated in the extraction chamber after cleaning with the second cleaning liquid into the cache chamber 111. It can be understood that the freeze-dried magnetic beads adsorbed with nucleic acid are retained in the extraction chamber due to adsorption on the inner wall of the extraction chamber, thereby avoiding the loss of freeze-dried magnetic beads. The valve assembly 210 is then rotated so that the second exchange port 2112 is located below the sample chamber, causing the piston 120 to move downward to inject the waste liquid in the cache chamber 111 into the sample chamber. At this time, there is no liquid in the extraction chamber, but only freeze-dried magnetic beads adsorbed with nucleic acid.

[0055] In step 11, step 10 can be repeated multiple times, so that the second cleaning solution can be used to clean the freeze-dried magnetic beads multiple times, thereby improving the cleaning effect of impurities and thus achieving nucleic acid cleaning. Of course, if step 10 only needs to be performed once, step 11 can be omitted, and the operation of step 12 below can be directly performed.

[0056] In step 12, the valve assembly 210 is rotated so that the second exchange port 2112 is located below the eluent chamber, causing the piston 120 to move upward, sucking the eluent in the eluent chamber into the buffer chamber 111. The valve assembly 210 is then rotated so that the second exchange port 2112 is located below the extraction chamber, causing the piston 120 to move downward, injecting the eluent in the buffer chamber 111 into the extraction chamber.

[0057] In the thirteenth step, an external ultrasonic device and a heating device are used to alternately contact the position of the shell component 110 corresponding to the extraction chamber. Under the action of ultrasonic waves and heat, the freeze-dried magnetic beads adsorbed with nucleic acids will be fully mixed and incubated with the eluent, so that the nucleic acids are eluted from the freeze-dried magnetic beads, that is, the nucleic acids are separated from the freeze-dried magnetic beads and integrated into the eluent. The eluent integrated with nucleic acids will form a nucleic acid product, thereby achieving the elution of nucleic acids.

[0058] In the fourteenth step, an external magnet is attached to the shell assembly 110, thereby adsorbing the freeze-dried magnetic beads that no longer contain nucleic acids to the inner wall of the extraction chamber. The piston 120 is then moved upward to draw the nucleic acid products in the extraction chamber into the buffer chamber 111. At this point, nucleic acid extraction from the sample is achieved.

[0059] Therefore, on the one hand, since the rotation of the valve assembly 210 can make the cache chamber 111 alternately connected with different accommodating chambers 112 through the same exchange channel 211, the liquid is exchanged between the cache chamber 111 and the different accommodating chambers 112, and finally the liquid in the different accommodating chambers 112 is exchanged through the cache chamber 111, avoiding the use of multiple pipes to exchange the liquid, thereby simplifying the structure of the processing device 10, reducing the manufacturing cost of the processing device 10 and improving the reliability. On the other hand, through the action of the liquid-isolating and breathable membrane 130, the aerosol and liquid in the accommodating chamber 112 are prevented from leaking out of the processing device 10, and foreign impurities are prevented from invading the cache chamber 111 or the accommodating chamber 112, thereby avoiding cross contamination. On the other hand, the liquid is transferred within the same processing device 10, avoiding the transfer of liquid between different devices, thereby reducing the transfer distance and time of the liquid, and thus improving the working efficiency of the processing device 10. At the same time, the transfer of the liquid can be automated by controlling the movement of the exchange valve 200 and the piston 120, avoiding manual intervention, thereby further improving the working efficiency of the processing device 10. It is understandable that, given that human involvement can be avoided in the process of liquid transfer, cross contamination between the liquid and the human body can also be avoided.

[0060] See Figure 4 、 Figure 5 and Figure 6 In some embodiments, the processing device 10 may further include a collection tube 400, which is connected to the shell assembly 110. The shell assembly 110 further defines a collection chamber 114. A plurality of accommodating chambers 112 and the collection chamber 114 are spaced apart around the buffer chamber 111. The collection chamber 114 can communicate with the lumen of the collection tube 400. The liquid-isolating and breathable membrane 130 can also cover the opening of the collection chamber 114 away from the exchange valve 200. When the valve assembly 210 rotates, the second exchange port 2112 can be located below the collection chamber 114 and aligned with the collection chamber 114, so that the second exchange port 2112 and the collection chamber 114 are connected to each other, thereby allowing the liquid in the buffer chamber 111 to enter the collection chamber 114.

[0061] Therefore, when the processing device 10 further includes a collection tube 400, after the above-mentioned fourteenth step is completed, the valve assembly 210 can be rotated so that the second exchange port 2112 is located below the collection chamber 114, and then the piston 120 is moved downward, so that the nucleic acid product in the buffer chamber 111 is injected into the lumen of the collection tube 400 through the exchange channel 211 and the collection chamber 114, thereby realizing the storage of the nucleic acid product in the collection tube 400. In this way, the automatic storage and collection function of the nucleic acid product of the processing device 10 is realized, effectively avoiding the processing device 10 from being unable to automatically store and collect the nucleic acid product, thereby increasing the diversification of the functions of the processing device 10 and ultimately improving the applicability of the processing device 10 to various working conditions.

[0062] See Figure 1 、 Figure 4 、 Figure 5 and Figure 6 In some embodiments, the housing assembly 110 includes a shell 117 and a cover 118. The cover 118 is connected to the end of the shell 117 away from the exchange valve 200. The shell 117 includes a bottom plate 1171, an inner tube 1172, an outer tube 1173, and a partition 1174. The inner tube 1172 and the outer tube 1173 are both protruded from the bottom plate 1171 along the thickness direction of the bottom plate 1171. Outer tube 1173 surrounds inner tube 1172, with partition 1174 located in the space between inner tube 1172 and outer tube 1173. Partition 1174 is connected to inner tube 1172 and / or inner tube 1172. Of course, the lower end of partition 1174 may also be connected to bottom plate 1171. This allows the space between partitions 1174 to be configured as part of accommodating chamber 112 or collection chamber 114, while the space enclosed by inner tube 1172 is configured as part of cache chamber 111. Second cache port 1112 and second accommodating port 1122 are located in cover 118. This allows cache chamber 111 and accommodating chamber 112 to be integrated into the same shell assembly 110, thereby simplifying the structure of processing device 10.

[0063] See Figure 1 、 Figure 4 、 Figure 5 and Figure 6In some embodiments, the cover body 118 includes a first cover 1181 and a second cover 1182. The first cover 1181 is fixedly connected to the housing 117, and the second cover 1182 is movably connected to the first cover 1181 to open or close the first cover 1181. A cache hole 1183 and a receiving hole 1184 are defined in the first cover 1181 and the second cover 1182. The cache hole 1183 is configured as part of the cache cavity 111, and the opening of the cache hole 1183 on the second cover 1182 is the second cache opening 1112. The receiving hole 1184 is configured as part of the receiving cavity 112, and the opening of the receiving hole 1184 on the second cover 1182 is the second receiving opening 1122. The liquid-barrier, breathable membrane 130 is positioned between the first cover 1181 and the second cover 1182, allowing it to block the cache hole 1183 and the receiving hole 1184, thereby blocking the second cache port 1112 and the second receiving port 1122. The liquid-barrier, breathable membrane 130 is fixedly connected to the second cover 1182. When the second cover 1182 opens the first cover 1181, interference with the liquid-barrier, breathable membrane 130 is avoided, facilitating the addition of liquid into the receiving chamber 112 through the receiving hole 1184 in the first cover 1181. When the second cover 1182 closes the first cover 1181, the liquid-barrier, breathable membrane 130 blocks the cache hole 1183 and the receiving hole 1184. This improves the ease of operation of the processing device 10.

[0064] See Figure 1 、 Figure 4 、 Figure 5 and Figure 6 In some embodiments, the second cover 1182 includes a rotating portion 1182a and a connecting portion 1182b. The rotating portion 1182a is rotatably connected to the first cover 1181, and the connecting portion 1182b is detachably connected to the first cover 1181, for example, the connecting portion 1182b is snap-fitted to the first cover 1181. When it is necessary to open the first cover 1181, the snap-fitting connection between the connecting portion 1182b and the first cover 1181 can be released, allowing the connecting portion 1182b to rotate away from the first cover 1181. When it is necessary to close the first cover 1181, the connecting portion 1182b can be rotated closer to the first cover 1181 until the connecting portion 1182b contacts the first cover 1181 and forms a snap-fit connection. In other embodiments, the second cover 1182 can be slidably connected to the first cover 1181, which can also allow the second cover 1182 to open or close the first cover 1181.

[0065] See Figure 2 、 Figure 6 and Figure 7In some embodiments, the shell assembly 110 further includes a seal 119, which is connected to the housing 117 and abuts between the valve assembly 210 and the bottom plate 1171. The first buffer port 1111 and the first receiving port 1121 are located within the seal 119. The seal 119 provides a certain degree of flexibility. When the seal 119 abuts the valve assembly 210, the gap between the seal 119 and the valve assembly 210 can be effectively eliminated. When the second exchange port 2112 is connected to the first receiving port 1121 of one of the receiving chambers 112, the valve assembly 210 can effectively block the first receiving port 1121 of the other receiving chamber 112, thereby preventing liquid in the other receiving chamber 112 from leaking through the first receiving port 1121. This can improve the reliability of the processing device 10.

[0066] See Figure 1 In some embodiments, the outer circumferential surface of the outer tube 1173 of the shell assembly 110 includes multiple flat surfaces 1175. These flat surfaces 1175 are spaced apart along the circumference of the outer tube 1173, and different flat surfaces 1175 correspond to the accommodating cavity 112. The provision of flat surfaces 1175 facilitates contact between the external ultrasonic device and the shell assembly 110, preventing the external ultrasonic device from slipping relative to the shell assembly 110. Furthermore, this also facilitates the installation of an external magnet, preventing the external magnet from slipping relative to the shell assembly 110.

[0067] See Figure 3 、 Figure 8 、 Figure 9 and Figure 10In some embodiments, the valve assembly 210 includes a driver 213 and a valve body 214. The valve body 214 can be stacked on the driver 213 so that the valve body 214 can abut between the driver 213 and the seal 119. The driver 213 has an exchange groove 2114 recessed on its surface facing the seal 119. The exchange groove 2114 can be curved. The valve body 214 is connected to the driver 213 and covers the opening of the exchange groove 2114. The valve body 214 is provided with a first exchange hole 2115 and a second exchange hole 2116. The first exchange hole 2115 and the second exchange hole 2116 penetrate the valve body 214 along its thickness. The first exchange hole 2115 and the second exchange hole 2116 are spaced apart and both communicate with the exchange groove 2114. The first exchange hole 2115 has an opening on the surface of the valve body 214 facing the seal 119, which is the first exchange port 2111. The second exchange hole 2116 has an opening on the surface of the valve body 214 facing the seal 119, which is the second exchange port 2112. The exchange groove 2114, the first exchange hole 2115, and the second exchange hole 2116 are configured as the exchange channel 211, so that the exchange channel 211 includes the exchange groove 2114, the first exchange hole 2115, and the second exchange hole 2116.

[0068] See Figure 3 、 Figure 8 、 Figure 9 and Figure 10 In some embodiments, the crossover valve 200 further includes a carrier 220, which is detachably connected to the shell assembly 110. For example, the carrier 220 can form a snap-fit connection with the outer cylinder 1173. The driver 213 abuts between the carrier 220 and the valve body 214, and the valve body 214 abuts against the seal 119. In this way, the entire valve assembly 210 abuts between the carrier 220 and the seal 119, thereby installing the valve assembly 210 and preventing the valve assembly 210 from sliding along the axial direction of the shell assembly 110. The valve assembly 210 can only rotate about an axis extending axially along the shell assembly 110.

[0069] See Figure 6 In some embodiments, the cache chamber 111 has a contracting section, which is located near the first cache opening 1111, specifically, near the seal 119. The diameter of the contracting section decreases from the second cache opening 1112 toward the first cache opening 1111. Specifically, the diameter of the contracting section may gradually decrease from top to bottom, resulting in a generally conical shape. This allows the contracting section to guide and converge the liquid in the cache chamber 111. During the downward movement of the piston 120, this ensures that the liquid is completely discharged from the cache chamber 111, preventing any liquid from remaining in the cache chamber 111.

[0070] See Figure 4 and Figure 5 In some embodiments, the cover 118 further includes a shielding film 1185. A connecting groove 1181a is formed on the first cover 1181. The connecting groove 1181a can be formed by recessing the surface of the first cover 1181 toward the second cover 1182 to a certain depth. The connecting groove 1181a can connect the collection chamber 114 and the lumen of the collection tube 400. The shielding film 1185 is used to cover the opening of the connecting groove 1181a, thereby forming a closed channel. When it is necessary to automatically collect the nucleic acid product extracted from the buffer chamber 111, the valve assembly 210 can be rotated so that the second exchange port 2112 is located below the collection chamber 114, and then the piston 120 is moved downward, so that the nucleic acid product in the buffer chamber 111 is injected into the lumen of the collection tube 400 through the exchange channel 211, the collection chamber 114, and the connecting groove 1181a in sequence, thereby achieving the collection of the nucleic acid product by the collection tube 400.

[0071] See Figure 4 and Figure 5 In some embodiments, the first cover 1181 may further include a protrusion 1181b. The protrusion 1181b may be substantially cylindrical. When the shielding film 1185 is connected to the first cover 1181, the protrusion 1181b may be inserted into the shielding film 1185, thereby ensuring proper positioning of the shielding film 1185 and improving the installation accuracy and connection strength of the shielding film 1185. Of course, the protrusion 1181b may also be inserted into the liquid-isolating and breathable membrane 130, thereby also effectively positioning the liquid-isolating and breathable membrane 130. The protrusions 1181b inserted into the shielding film 1185 and the liquid-isolating and breathable membrane 130 may be different or the same.

[0072] See Figure 4 and Figure 5 In some embodiments, the cover 118 of the housing assembly 110 further includes a ventilation hole 1186 that connects the lumen of the collection tube 400 with the outside world. By providing ventilation holes 1186, when liquids such as nucleic acid products enter the lumen of the collection tube 400 from the collection chamber 114 and the connecting groove 1181a, the gas in the lumen of the collection tube 400 can be discharged to the outside world through ventilation holes 1186, making the air pressure in the collection tube 400 equivalent to the outside atmospheric pressure, thereby ensuring that liquids such as nucleic acid products can smoothly enter the lumen of the collection tube 400.

[0073] See Figure 2 、 Figure 3 and Figure 11In some embodiments, the processing device 10 further includes a reaction mechanism 300, which is detachably connected to the outer tube 1173 of the shell assembly 110, for example, by snapping the reaction mechanism 300 into the outer tube 1173. The reaction mechanism 300 defines a reaction chamber 330 and a first reaction channel 310, with the reaction chamber 330 and the first reaction channel 310 communicating with the outside world. The shell assembly 110 defines a first flow channel 115, which is always in communication with the first flow channel 310. When the valve assembly 210 rotates, the second exchange port 2112 can communicate with the first flow channel 115 and different accommodating chambers 112. Therefore, when the second exchange port 2112 is located below the first flow channel 115, it aligns with the end opening of the first flow channel 115, thereby connecting the second exchange port 2112 to the reaction chamber 330 through the first flow channel 115 and the first reaction channel 310. The multiple accommodating chambers 112 may further include a PCR reagent chamber, which is used to hold PCR reagents.

[0074] See Figure 2 、 Figure 3 and Figure 11 After the nucleic acid product in the extraction chamber is drawn into the buffer chamber 111 through step 14, if it is not necessary to transfer the nucleic acid product to the collection tube 400 through the collection chamber 114 for storage, the nucleic acid can be transferred to the reaction chamber 330 for amplification reaction for subsequent fluorescence detection. The operation steps are as follows:

[0075] First, rotate the valve assembly 210 so that the second exchange port 2112 is aligned with the PCR reagent chamber, thereby achieving communication between the exchange channel 211 and the PCR reagent chamber. Drive the piston 120 downward to input the nucleic acid product in the cache chamber 111 into the PCR reagent chamber through the exchange channel 211, so that the nucleic acid product is mixed with the PCR reagent in the PCR reagent chamber. Of course, to ensure the uniformity of the mixing, the piston 120 can be repeatedly moved up and down to cause turbulence between the nucleic acid product and the PCR reagent and to fully mix them. After the mixing is completed, the piston 120 can be driven to move upward to suck the mixed solution formed by the nucleic acid product and the PCR reagent in the PCR reagent chamber into the cache chamber 111.

[0076] Then, the valve assembly 210 is rotated so that the second exchange port 2112 is aligned with the first flow channel 115, thereby achieving communication between the exchange channel 211 and the first flow channel 115. The piston 120 is driven downward to transfer the mixed solution in the buffer chamber 111 to the reaction chamber 330 through the exchange channel 211, the first flow channel 115, and the first reaction channel 310 in sequence.

[0077] Finally, the reaction mechanism 300 is inserted into an external PCR device, and the amplification program is started. After the amplification is completed, fluorescence detection can be performed.

[0078] Therefore, by setting up the reaction mechanism 300, after the extraction of the nucleic acid product is completed, the nucleic acid product can be directly input into the reaction mechanism 300 for amplification reaction to achieve fluorescence detection, so that the extraction and detection of nucleic acid are completed on the same processing device 10, that is, the processing device 10 has the functions of extraction and detection of nucleic acid at the same time, which can also improve the diversification of the functions of the processing device 10, and ultimately improve the applicability of the processing device 10 to various working conditions. It can be understood that the extracted nucleic acid product can directly enter the reaction mechanism 300 for reaction for fluorescence detection, without the need to remove the nucleic acid product from the processing device 10 to transfer it to other detection devices for fluorescence detection, and ensure that the nucleic acid product is transferred within the processing device 10. In this way, the transfer distance and transfer time of the nucleic acid product can be reduced, thereby improving the working efficiency of the processing device 10. Of course, the transfer of the nucleic acid product is completed in the closed processing device 10, which can effectively avoid the contamination of the nucleic acid product caused by the process between the processing device 10 and the external detection device, thereby providing the reliability of the processing device 10.

[0079] See Figure 2 、 Figure 3 and Figure 11 In some embodiments, the shell assembly 110 is further provided with a second flow guide channel 116 and a ventilation cavity 113. The second flow guide channel 116 and the ventilation cavity 113 are spaced apart, and the ventilation cavity 113 passes through the cover 118, so that the ventilation cavity 113 forms an opening on the cover 118 that is connected to the outside world, and the liquid-isolating and breathable membrane 130 can block the opening. The reaction mechanism 300 is further provided with a second reaction channel 320, and the second reaction channel 320 is always in a connected state with the reaction cavity 330 and the second flow guide channel 116. When the second exchange port 2112 is connected with the first flow guide channel 115, the ventilation cavity 113 can be connected with the second flow guide channel 116. For example, the valve assembly 210 is further provided with a transfer channel 212, and the transfer channel 212 and the exchange channel 211 are independent of each other and do not connect with each other. The transfer channel 212 has a first transfer port 2121 and a second transfer port 2122, which are spaced apart. When the second exchange port 2112 is connected to the first flow channel 115, the first transfer port 2121 is connected to the ventilation cavity 113, and the second transfer port 2122 is connected to the second flow channel 116. This ensures that the ventilation cavity 113 and the second flow channel 116 are connected through the transfer channel 212.

[0080] Therefore, when the second exchange port 2112 is connected to the first guide channel 115, on the one hand, the cache chamber 111 is connected to the reaction chamber 330 via the channel exchange channel 211, the first guide channel 115 and the first reaction channel 310 in sequence, and on the other hand, the ventilation chamber 113 is connected to the reaction chamber 330 via the transfer channel 212, the second guide channel 116 and the second reaction channel 320 in sequence, so that the reaction chamber 330 can be connected to the outside world through the second reaction channel 320, the second guide channel 116, the transfer channel 212 and the ventilation chamber 113, that is, the external gas can enter the reaction chamber 330 through the ventilation chamber 113, so that the air pressure on the side of the reaction chamber 330 close to the second reaction channel 320 is equivalent to the atmospheric pressure. Therefore, when the liquid in the buffer chamber 111 enters the reaction chamber 330, the gas in the reaction chamber 330 can be discharged to the outside through the second reaction channel 320, the second flow guide channel 116, the transfer channel 212, and the ventilation chamber 113 in sequence, thereby preventing the gas pressure in the reaction chamber 330 from increasing and ensuring that the liquid in the buffer chamber 111 smoothly enters the reaction chamber 330. Of course, when the second exchange port 2112 and the first flow guide channel 115 are misaligned and not connected to each other, the first transfer port 2121 and the ventilation chamber 113 are misaligned and not connected to each other, and the second transfer port 2122 and the second flow guide channel 116 are misaligned and not connected to each other.

[0081] It is understood that when the liquid undergoes an amplification reaction in the reaction mechanism 300, the second exchange port 2112 and the first flow channel 115 can be offset and disconnected from each other, the first transfer port 2121 and the ventilation cavity 113 can be offset and disconnected from each other, and the second transfer port 2122 and the second flow channel 116 can be offset and disconnected from each other. This allows the seal 119 to block the end openings of the first flow channel 115 and the second flow channel 116, thereby preventing the reaction chamber 330 from communicating with the outside world and the buffer chamber 111, preventing liquid from leaking within the reaction chamber 330, and improving the reliability of the amplification reaction.

[0082] In other embodiments, in order to connect the reaction chamber 330 to the outside world so that the buffer chamber 111 can smoothly inject the liquid into the reaction chamber 330, an air passage connecting the reaction chamber 330 can be directly opened on the reaction mechanism 300, so that the air passage is connected to the outside world. In this way, the gas in the reaction chamber 330 can also be discharged through the air passage, ensuring that the buffer chamber 111 can smoothly inject the liquid into the reaction chamber 330.

[0083] See Figure 8 、 Figure 9 、 Figure 10 and Figure 11In some embodiments, a transfer groove 2123 is defined on the driver 213. The valve body 214 is connected to the driver 213 and covers the opening of the transfer groove 2123. The valve body 214 is provided with a first transfer hole 2124 and a second transfer hole 2125. The first transfer hole 2124 and the second transfer hole 2125 are spaced apart and connected to the transfer groove 2123. The transfer groove 2123, the first transfer hole 2124, and the second transfer hole 2125 are configured as the transfer channel 212. That is, the transfer channel 212 includes the transfer groove 2123, the first transfer hole 2124, and the second transfer hole 2125. The first transfer port 2121 is located in the first transfer hole 2124, and the second transfer port 2122 is located in the second transfer hole 2125. In other embodiments, the first transfer hole 2124 and the second transfer hole 2125 can overlap with each other to form the same hole, so that the ventilation cavity 113 and the second guide channel 116 are connected to the transfer groove 2123 through the same hole, that is, the ventilation cavity 113 and the second guide channel 116 are connected to the transfer channel 212 through the same position on the transfer channel 212.

[0084] See Figure 8 、 Figure 9 、 Figure 10 and Figure 11 In some embodiments, the exchange channel 211 further includes a pressurizing port 2113, which is spaced apart from the first exchange port 2111 and the second exchange port 2112. For example, the pressurizing port 2113 is spaced apart from the second exchange port 2112 on the circumference formed by the rotation of the second exchange port 2112. When the valve assembly 210 rotates, the pressurizing port 2113 can communicate with the first flow guide channel 115. At this time, the second exchange port 2112 is misaligned with the first flow guide channel 115, and the seal 119 blocks the vent cavity 113, the second flow guide channel 116, the second exchange port 2112, the first transfer port 2121, and the second transfer port 2122. Gas in the reaction chamber 330 cannot be discharged to the outside through the vent cavity 113. When the piston 120 slides downward, the gas in the buffer chamber 111 can enter the reaction chamber 330 through the pressurization port 2113 of the exchange channel 211, the first guide channel 115 and the first reaction channel 310 in sequence. Since the gas in the reaction chamber 330 cannot be discharged to the outside through the ventilation chamber 113, the gas pressure in the reaction chamber 330 increases.

[0085] See Figure 3 and Figure 11In some embodiments, the reaction mechanism 300 includes a reaction plate 340 and a covering film 350. The reaction plate 340 is connected to the shell assembly 110. The first reaction channel 310 and the second reaction channel 320 can be disposed within the reaction plate 340. The reaction chamber 330 penetrates the reaction plate 340 along the thickness direction of the reaction plate 340. There are two covering films 350, which are located on opposite sides of the reaction plate 340 in the thickness direction. The reaction chamber 330 is located between the two covering films 350. The two covering films 350 thus block the two openings of the reaction chamber 330 in the thickness direction of the reaction plate 340, thereby sealing the reaction chamber 330.

[0086] When the liquid undergoes an amplification reaction in the reaction chamber 330, an external heating plate can be placed against the cover film 350 and the reaction plate 340 to maintain the liquid amplification reaction at a certain temperature. Given the relatively small thickness of the reaction plate 340 and the cover film 350, the heat conduction rate of the reaction plate 340 and the cover film 350 can be increased, allowing the heat from the heating plate to be quickly transferred through the reaction plate 340 and the cover film 350 to the liquid in the reaction chamber 330. This increases the heat conduction rate and reduces heat damage. This can increase the speed of the amplification reaction, thereby improving the operating efficiency of the processing device 10.

[0087] During the heating process through the heating plate, the pressurizing port 2113 can be connected to the first guide channel 115, and the piston 120 can move downward, so that the gas in the buffer chamber 111 enters the reaction chamber 330. The air pressure in the reaction chamber 330 will increase, causing the covering film 350 on both sides of the reaction plate 340 to expand outward, so that the covering film 350 fits tightly with the heating plate, ensuring that the heat of the heating plate is quickly transferred to the liquid in the reaction chamber 330 through the covering film 350, thereby further improving the speed of the amplification reaction and the working efficiency of the processing device 10.

[0088] In some embodiments, the reaction mechanism 300 further includes a connector 360, which is detachably connected to the shell assembly 110, for example, the connector 360 is snap-fitted to the shell assembly 110, and the reaction plate 340 is inserted into and fixedly connected to the connector 360. Of course, the reaction mechanism 300 may further include a sealing ring, which abuts between the reaction plate 340 and the shell assembly 110, so that the sealing ring seals the connection between the first reaction channel 310 and the first flow guide channel 115, and also seals the connection between the second reaction channel 320 and the second flow guide channel 116.

[0089] See Figure 8 、 Figure 9 、 Figure 10 and Figure 11In some embodiments, the number of reaction mechanisms 300 may be two, and the two reaction mechanisms 300 may be symmetrically arranged relative to the shell assembly 110. In addition to performing amplification reactions for fluorescence detection, the reaction mechanism 300 may also perform library construction through diffusion reactions. Therefore, the processing device 10 may integrate multiple functions such as nucleic acid extraction, nucleic acid detection, and library construction, thereby further improving the diversity of the functions of the processing device 10, thereby improving the applicability of the processing device 10 to various working conditions. When the processing device 10 performs library construction, the processing device 10 may execute the library construction processes of NGS, tNGS, and third-generation sequencing; that is, the processing device 10 may be compatible with the library construction processes of the second-generation sequencing represented by NGS and tNGS, and may also be compatible with the library construction processes of the third-generation sequencing.

[0090] The multiple accommodating chambers 112 may also include a first PCR reagent chamber, a second PCR reagent chamber, a first purification chamber, a second purification chamber, and an ethanol chamber. The following is a brief description of the tNGS library construction process:

[0091] After the fourteenth step in the nucleic acid extraction process is performed and the nucleic acid product is transferred to the cache chamber 111, the following operations can be performed in sequence through the rotation of the valve assembly 210 and the up and down movement of the piston 120: the nucleic acid product is transferred from the cache chamber 111 to the first PCR reagent chamber for mixing, the nucleic acid product mixed with the first PCR reagent is transferred to the cache chamber 111, the nucleic acid product mixed with the first PCR reagent is transferred from the cache chamber 111 to the reaction chamber 330 of one of the reaction mechanisms 300 for amplification reaction, and the amplified product in the reaction chamber 330 is transferred to the cache chamber 111. , transfer the amplification product to the first purification chamber, transfer the waste liquid from the first purification chamber to the cache chamber 111, transfer the waste liquid from the cache chamber 111 to the sample chamber, transfer ethanol from the ethanol chamber to the cache chamber 111, transfer ethanol from the cache chamber 111 to the first purification chamber, transfer the waste liquid from the first purification chamber to the cache chamber 111, transfer the waste liquid from the cache chamber 111 to the sample chamber, transfer ethanol from the ethanol chamber to the cache chamber 111, transfer ethanol from the cache chamber 111 to the first purification chamber, transfer the waste liquid from the first purification chamber to the cache chamber 111, and transfer the waste liquid from the cache chamber 111 to the sample chamber. Transfer the eluent from the elution chamber to the cache chamber 111, transfer the eluent from the cache chamber 111 to the first purification chamber, transfer the elution product from the first purification chamber to the cache chamber 111, transfer the elution product from the cache chamber 111 to the second PCR reagent chamber, transfer the elution product mixed with the second PCR reagent from the second PCR reagent chamber to the cache chamber 111, transfer the elution product mixed with the second PCR reagent from the cache chamber 111 to the reaction chamber 330 of another reaction mechanism 300 for amplification reaction, transfer the amplification product from the reaction chamber 330 to the cache chamber 111, transfer the amplification product from the cache chamber 111 to the second purification chamber, transfer the waste liquid from the second purification chamber to the cache chamber 111, transfer the waste liquid from the cache chamber 111 to the sample chamber, transfer ethanol from the ethanol chamber to the cache chamber 111, transfer ethanol from the cache chamber 111 to the second purification chamber, transfer the waste liquid from the second purification chamber to the cache chamber 111, transfer the waste liquid from the cache chamber 111 to the sample chamber. Transfer ethanol from the ethanol chamber to the buffer chamber 111, transfer ethanol from the buffer chamber 111 to the second purification chamber, transfer waste liquid from the second purification chamber to the buffer chamber 111, and transfer waste liquid from the buffer chamber 111 to the sample chamber. Transfer an eluent, such as enzyme-free water, from the elution chamber to the buffer chamber 111, transfer enzyme-free water from the buffer chamber 111 to the second purification chamber, and transfer the eluted product from the second purification chamber to the buffer chamber 111. At this point, the eluted product in the buffer chamber 111 is actually the library product. The library product is then transferred from the buffer chamber 111 to the collection tube 400 via the collection chamber 114.

[0092] Therefore, by providing the processing device 10 with a collection tube 400 and a reaction mechanism 300, when the nucleic acid product is not subjected to an amplification reaction for fluorescence detection by the reaction mechanism 300, the nucleic acid product can be input into the collection tube 400 for storage. The reaction mechanism 300 can not only perform an amplification reaction on the nucleic acid product for fluorescence detection, but also perform an amplification reaction on the nucleic acid product for library construction, so that the library product can be collected by the collection tube 400. Therefore, the collection tube 400 can collect both nucleic acid products and library products.

[0093] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0094] The above-described embodiments merely represent several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that a person of ordinary skill in the art may make various modifications and improvements without departing from the spirit of the present application, and these modifications and improvements fall within the scope of protection of the present application. Therefore, the scope of protection of the present patent application shall be determined by the appended claims.

Claims

1. A processing device, characterized in that include: A main unit, comprising a shell assembly, a piston, and a liquid-isolating, breathable membrane; the shell assembly is provided with a cache chamber and a plurality of accommodating chambers extending axially through the shell assembly; the two ends of the cache chamber respectively form a first cache opening and a second cache opening; the two ends of the accommodating chamber respectively form a first accommodating opening and a second accommodating opening; the liquid-isolating, breathable membrane is provided on the shell assembly and covers the second cache opening and the second accommodating opening; the piston is slidably provided within the cache chamber; and An exchange valve includes a valve assembly rotatably connected to the shell assembly, the valve assembly is provided with an exchange channel, the exchange channel has a first exchange port and a second exchange port spaced apart, when the valve assembly rotates, the first exchange port is always connected to the first cache port, and the second exchange port is alternately connected to different first accommodating ports.

2. The processing device according to claim 1, characterized in that The shell assembly includes a shell and a cover body, the cover body is connected to one end of the shell away from the exchange valve, the shell includes a bottom plate, an inner tube, an outer tube and a partition, the inner tube and the outer tube are both protrudingly arranged on the bottom plate, the outer tube is arranged around the inner tube, the partition is connected to the inner tube and / or the inner tube, the space enclosed by the inner tube is configured as a part of the cache cavity, the space between the partitions is configured as a part of the accommodating cavity, and the second cache port and the second accommodating port are located on the cover body.

3. The processing device according to claim 2, characterized in that The cover body includes a first cover and a second cover, the first cover is fixedly connected to the shell, and the second cover is movably connected to the first cover to open or close the first cover, and a cache hole and a receiving hole are provided on the first cover and the second cover, the cache hole is configured as a part of the cache cavity and has the second cache port located in the second cover, and the receiving hole is configured as a part of the receiving cavity and has the second receiving port located in the second cover, and the liquid-isolating and breathable membrane is located between the first cover and the second cover and can block the cache hole and the receiving hole.

4. The processing device according to claim 3, characterized in that The liquid-isolating and breathable membrane is fixedly connected to the second cover.

5. The processing device according to claim 3, characterized in that The second cover includes a rotating portion and a connecting portion, the rotating portion is rotatably connected to the first cover, and the connecting portion is detachably connected to the first cover.

6. The processing device according to claim 2, characterized in that The shell assembly further includes a sealing member connected to the shell body, the sealing member abuts between the valve assembly and the bottom plate, and the first cache port and the first accommodating port are located on the sealing member.

7. The processing device according to claim 1, characterized in that The outer peripheral surface of the shell component includes a plurality of planes, and the plurality of planes are arranged at intervals along the circumference of the shell component, and different planes correspond to different accommodating cavities.

8. The processing device according to claim 1, characterized in that The valve assembly includes a driving member and a valve body, the driving member is provided with an exchange groove, the valve body is connected to the driving member and covers the opening of the exchange groove, the valve body is provided with a first exchange hole and a second exchange hole connected to the exchange groove at intervals, the exchange groove, the first exchange hole and the second exchange hole are configured as the exchange channel, the first exchange port is located in the first exchange hole, and the second exchange port is located in the second exchange hole.

9. The processing device according to claim 8, characterized in that The exchange valve further includes a bearing member, which is detachably connected to the shell assembly. The driving member abuts between the bearing member and the valve body, and the valve body abuts against the shell assembly.

10. The processing device according to claim 1, characterized in that Also includes at least one of the following options: The orthographic projections of the different first receiving ports all fall on the circumference formed by the rotation of the second exchange port; The cache cavity has a contraction section arranged close to the first cache opening, and the diameter of the contraction section decreases from the second cache opening to the first cache opening.

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