A microbial detection assay device

Abstract

The application provides a kind of microbial detection analysis device, it is related to the field of microbial detection analysis, comprising: container assembly, the container assembly includes medicament storage and piston mechanism, control component can be linked piston mechanism to realize the operation of liquid injection to the inside of culture dish, under the control of control component, the fixed injection amount of piston mechanism each time, can quickly prepare a large number of same samples for analysis and detection use, greatly improve the accuracy of microbial detection analysis result, at the same time, the amount of single injection of piston mechanism can also be regulated, can adapt to different microbial analysis and detection sample preparation operation etc., solve the problem that multiple samples are prepared in the existing microbial analysis and detection, corresponding liquid medicine needs to be injected into the inside of culture dish by liquid injector manually, liquid injector does not have the function of quantitative liquid injection, the amount of liquid medicine in the inside of culture dish is not the same, easy to interfere with the result of microbial detection analysis, the problem of poor accuracy.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microorganism detection and analysis, and particularly relates to a microorganism detection and analysis device. BACKGROUND

[0002] Microorganism detection is a process of qualitatively and quantitatively analyzing microorganisms in samples by using various methods. In actual detection and analysis, parallel experiment design is usually used to ensure the accuracy and reliability of the results, and to exclude experimental errors and accidental factors interference. Multiple groups of samples are prepared for synchronous detection and analysis.

[0003] In the current field of microorganism analysis and detection, the preparation process of multiple groups of samples usually relies on manual operation of a liquid injector to inject liquid medicine into a culture dish. Most of the existing liquid injectors lack quantitative liquid injection function, and the injection amount mainly depends on the experience and manual control of the operator. Due to the differences in operation methods of different operators, operation fatigue, and errors in visual observation, etc., it is easy to cause significant deviation in the amount of liquid medicine in each culture dish. This inconsistency in the amount of liquid medicine in the sample preparation process will interfere with the growth environment and metabolic process of microorganisms, thereby causing adverse effects on the subsequent detection and analysis results, reducing the accuracy and reliability of the detection results, and having low practicality. SUMMARY

[0004] The present application relates to a microorganism detection and analysis device, which has a container assembly and a quantity control assembly. The interior of the medicament storage seat can store the liquid medicine required for the culture, analysis and detection of microorganisms, avoiding the need to frequently pump liquid medicine for standby use during use. The quantity control assembly can drive the piston mechanism to realize the operation of injecting liquid into the culture dish. Under the control of the quantity control assembly, the injection amount of the piston mechanism is fixed each time, which can quickly prepare a large number of identical samples for analysis and detection, greatly improving the accuracy of the microorganism detection and analysis results, simplifying the operation process, improving the efficiency of analysis and detection operations, etc. At the same time, the amount of single injection of the piston mechanism can also be adjusted, which can adapt to the sample preparation and other operations during different microorganism analysis and detection, and has high flexibility, adaptability and practicality.

[0005] The application provides a microbial detection and analysis device, which specifically comprises a container assembly and a quantity control assembly, the container assembly comprises a medicament storage and a piston mechanism; the piston mechanism comprises a piston rod, a piston block, a one-way ratchet and a linkage gear, the piston rod is inserted into the top of the medicament storage, the piston block is inserted into the piston cavity of the medicament storage, the outer ring of the one-way ratchet is fixedly installed on the top of the piston block, the inner ring of the one-way ratchet is fixedly installed on the bottom of the piston rod, and the linkage gear is rotationally connected to the inside of the medicament storage; the quantity control assembly comprises a pressing seat, a driving seat, a trigger block, a positioning block, a driving gear, a driving column and a positioning screw, the pressing seat, the driving seat and the driving column are sequentially inserted into the inside of the medicament storage from top to bottom, the driving seat is inserted into the bottom of the pressing seat, the trigger block is inserted into the inside of the driving seat, the positioning block is inserted into the inside of the driving seat, the driving gear is rotationally connected to the inside of the medicament storage, the gear teeth of the driving gear and the linkage gear are in meshing transmission, the driving column is inserted into the bottom of the driving seat, and the positioning screw is rotationally connected to the inside of the driving column and is screwed into the bottom of the driving seat through the rod body.

[0006] Further, the bottom of the pressing seat is provided with a trigger resisting rod, the trigger resisting rod is located directly above the trigger block, the bottom of the pressing seat is provided with a liquid injection top spring, and the two ends of the liquid injection top spring are respectively resisted against the bottom of the pressing seat and the top of the driving seat.

[0007] Further, the bottom of the trigger block is provided with a positioning top spring, and the two ends of the positioning top spring are respectively resisted against the inside of the trigger block and the inside of the driving seat.

[0008] Further, the inside of the trigger block is provided with an inclined control groove, the inside of the positioning block is provided with a control rod, the control rod is inserted into the inside of the control groove, the inside of the medicament storage is provided with a positioning clamping groove, and the positioning block is inserted into the inside of the positioning clamping groove.

[0009] Further, the outside of the driving column is provided with a driving groove, and the inside of the driving gear is provided with a driving protrusion, the driving protrusion is inserted into the inside of the driving groove.

[0010] Further, the driving groove is composed of a regulation groove arranged along the axis of the driving column and a power groove arranged spirally, and the bottom end of the power groove is connected with the top end of the regulation groove.

[0011] Further, the outside of the piston rod is provided with a linkage groove spirally extending, and the inside of the linkage gear is provided with a linkage protrusion, the linkage protrusion is inserted into the inside of the linkage groove.

[0012] Further, the piston block can only move up and down linearly in the piston cavity, and the bottom of the driving seat is provided with a track column with a cross section of a regular polygon, and the track column is inserted into the inside of the driving column.

[0013] Further, the bottom of the driving column is provided with a reset top spring, and two ends of the reset top spring are respectively abutted against the bottom of the driving column and the inside of the medicine storage seat, and the elastic force of the reset top spring is smaller than the elastic force of the liquid injection top spring.

[0014] The present application provides a kind of microbial detection analysis device, with the following beneficial effects:

[0015] The inside of the medicine storage seat can reserve the liquid medicine required for culture, analysis and detection of microorganisms, avoiding the need for frequent pumping of liquid medicine for standby during use, and the control assembly can be linked with the piston mechanism to realize the operation of liquid injection into the inside of the culture dish. Under the control of the control assembly, the liquid injection amount of the piston mechanism is fixed each time, which can quickly prepare a large number of identical samples for analysis and detection, greatly improving the accuracy of microbial detection and analysis results, simplifying the operation process, improving the efficiency of analysis and detection operations. At the same time, the amount of single injection of the piston mechanism can also be adjusted, which can adapt to the sample preparation and other operations during different microbial analysis and detection, improving the flexibility, adaptability and practicality of the device. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings of the embodiments will be briefly introduced below.

[0017] The drawings described below only relate to some embodiments of the present application, but not limit the present application.

[0018] In the drawings:

[0019] Figure 1 The structure of the present application is shown.

[0020] Figure 2 The structure of the present application is shown.

[0021] Figure 3 The structure of the present application is shown. Figure 2 The structure of the present application is shown.

[0022] Figure 4 The structure of the present application is shown.

[0023] Figure 5 The structure of the present application is shown.

[0024] Figure 6 The structure of the present application is shown. Figure 2 The structure of the present application is shown.

[0025] Figure 7 The structure of the present application is shown. Figure 6 The structure of the present application is shown.

[0026] Figure 8 The present application is shown Figure 6 The internal structure of the container assembly after a single injection operation is completed.

[0027] Figure 9 The present application is shown Figure 8 The structure of the C part is enlarged.

[0028] Figure 10 The present application is shown Figure 8 The internal structure of the control assembly after reset.

[0029] Figure 11 The present application is shown Figure 10 The structure of the D part is enlarged.

[0030] List of reference signs

[0031] 1, medicine storage; 101, piston cavity; 102, positioning card slot;

[0032] 2, piston mechanism; 201, piston rod; 2011, linkage groove; 202, piston block; 203, one-way ratchet; 204, linkage gear; 2041, linkage protrusion;

[0033] 3, control assembly; 301, pressing seat; 3011, trigger resistance rod; 3012, injection top spring; 302, drive seat; 3021, track column; 303, trigger block; 3031, positioning top spring; 3032, control groove; 304, positioning block; 3041, control rod; 305, drive gear; 3051, drive protrusion; 306, drive column; 3061, regulation groove; 3062, power groove; 3063, reset top spring; 307, positioning screw. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the embodiments of the present application will be described clearly and completely below with reference to the drawings of the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the described embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0035] Please refer to Figures 1 to 11 : Example one:

[0036] The application provides a microbial detection and analysis device, which comprises a container assembly and a quantity control assembly 3, the container assembly comprises a medicament storage 1 and a piston mechanism 2; the piston mechanism 2 comprises a piston rod 201, a piston block 202, a one-way ratchet wheel 203 and a linkage gear 204, the piston rod 201 is inserted into the top of the medicament storage 1, the piston block 202 is inserted into the piston cavity 101 of the medicament storage 1, the outer ring of the one-way ratchet wheel 203 is fixedly installed on the top of the piston block 202, the inner ring of the one-way ratchet wheel 203 is fixedly installed on the bottom of the piston rod 201, and the linkage gear 204 is rotationally connected to the inside of the medicament storage 1; the quantity control assembly 3 comprises a pressing seat 301, a driving seat 302, a trigger block 303, a positioning block 304, a driving gear 305, a driving column 306 and a positioning screw 307, the pressing seat 301, the driving seat 302 and the driving column 306 are sequentially inserted into the inside of the medicament storage 1 from top to bottom, the driving seat 302 is inserted into the bottom of the pressing seat 301, the trigger block 303 is inserted into the inside of the driving seat 302, the positioning block 304 is inserted into the inside of the driving seat 302, the driving gear 305 is rotationally connected to the inside of the medicament storage 1, the driving gear 305 and the linkage gear 204 are in gear transmission, the driving column 306 is inserted into the bottom of the driving seat 302, and the positioning screw 307 is rotationally connected to the inside of the driving column 306 and is screwed into the bottom of the driving seat 302 through the rod body thread.

[0037] In use, the inside of the piston cavity 101 can extract the medicinal liquid for standby, frequent extraction of the medicinal liquid for standby can be avoided in use, and convenience is achieved; when it is necessary to reserve the medicinal liquid for standby, the piston rod 201 only needs to be lifted, the piston rod 201 can drive the piston block 202 to move upward synchronously and extract the medicinal liquid into the inside of the piston cavity 101, and in the process, under the cooperation of the linkage protrusion 2041 and the linkage groove 2011, the piston rod 201 can reverse when being lifted, the inner ring of the one-way ratchet wheel 203 can rotate independently and will not jam the device when reversing, and the piston rod 201 will not drive the quantity control assembly 3 when being lifted, the stable extraction of the medicinal liquid for standby is ensured, and stable use is achieved.

[0038] The bottom of the pressing seat 301 is provided with a trigger abutting rod 3011 which is located directly above the trigger block 303, and the bottom of the pressing seat 301 is provided with a liquid injection top spring 3012, and the two ends of the liquid injection top spring 3012 are respectively abutted on the bottom of the pressing seat 301 and the top of the driving seat 302. In use, the liquid injection operation of the container assembly can be realized by the control assembly 3, and the liquid injection amount of the container assembly is the same each time, and the control assembly 3 adopts instantaneous transmission control, which can effectively avoid the misdischarge phenomenon caused by the pressing seat 301 not being pressed in place, and is stable and efficient in use. When injecting liquid, you only need to pull down the pressing seat 301, the bottom of the trigger block 303 is provided with a positioning top spring 3031, and the two ends of the positioning top spring 3031 are respectively abutted on the inside of the trigger block 303 and the inside of the driving seat 302. Due to the action of the positioning top spring 3031, the positioning block 304 is inserted into the inside of the positioning clamping groove 102, so that the use position of the driving seat 302 is fixed, so that the pressing seat 301 can be compressed when being pulled down. The liquid injection top spring 3012 stores energy, with the downward movement of the pressing seat 301, the trigger abutting rod 3011 will drive the trigger block 303 to move downward and compress the positioning top spring 3031 by abutting and extruding the trigger block 303. The inside of the trigger block 303 is provided with an inclined control groove 3032, and the inside of the positioning block 304 is provided with a control rod 3041, the control rod 3041 is inserted into the inside of the control groove 3032, the inside of the medicament storage seat 1 is provided with a positioning clamping groove 102, and the positioning block 304 is inserted into the inside of the positioning clamping groove 102. When the trigger block 303 moves downward, the control groove 3032 can drive the positioning block 304 to shrink into the inside of the driving seat 302 through the control rod 3041, so that the positioning block 304 is out of the inside of the positioning clamping groove 102, and the positioning function of the driving seat 302 through the positioning block 304 is also invalid. Thereafter, under the action of the liquid injection top spring 3012, the driving seat 302 can drive the driving column 306 to move downward and compress the reset top spring 3063 through the positioning screw 307, and complete the subsequent linkage injection action, which is convenient and flexible to use.

[0039] The outer part of the driving column 306 is provided with a driving groove, and the inner part of the driving gear 305 is provided with a driving protrusion 3051 which is inserted into the inner part of the driving groove. The driving groove is composed of a regulating groove 3061 which is arranged along the axial direction of the driving column 306 and a power groove 3062 which is arranged spirally. The bottom end of the power groove 3062 is connected with the top end of the regulating groove 3061. In use, when the driving column 306 moves downward, the driving protrusion 3051 will enter into the inner part of the power groove 3062 from the regulating groove 3061. When the driving protrusion 3051 moves in the inner part of the regulating groove 3061, it will not drive the driving gear 305 to rotate. After the driving protrusion 3051 enters into the inner part of the power groove 3062, the driving gear 305 will rotate with the downward movement of the driving column 306. When the driving gear 305 rotates, it can drive the linkage gear 204 to rotate. When the linkage gear 204 rotates, the linkage protrusion 2041 can make the piston rod 201 have a tendency of forward rotation through the linkage groove 2011. However, the inner and outer rings of the one-way ratchet 203 will be clamped with each other in the forward rotation direction of the piston rod 201, so that the piston rod 201 cannot rotate forward. Therefore, when the linkage gear 204 rotates, the linkage protrusion 2041 can drive the piston rod 201 to move downward through the linkage groove 2011. When the piston rod 201 moves downward, it can drive the piston block 202 to move downward synchronously, so as to discharge the liquid medicine in the inner part of the piston cavity 101. The use is convenient. The bottom part of the driving column 306 is provided with a reset compression spring 3063, and the two ends of the reset compression spring 3063 are respectively abutted against the bottom part of the driving column 306 and the inner part of the medicine storage 1. The elastic force of the reset compression spring 3063 is smaller than that of the liquid injection compression spring 3012. After the single liquid injection is completed, the pressing seat 301 is loosened, and under the action of the reset compression spring 3063, the control assembly 3 can move upward and reset as a whole, so as to be ready for subsequent liquid injection. In the upward resetting process of the control assembly 3, the linkage gear 204 will drive the piston rod 201 to rotate reversely (i.e. the inner ring of the one-way ratchet 203 idles), and will not drive the piston block 202 to move upward. The use is stable.

[0040] The piston block 202 can only move up and down linearly inside the piston cavity 101, and the bottom of the driving seat 302 is provided with a track column 3021 with a cross section of a regular polygon, which is inserted into the inside of the driving column 306. In use, since the moving distance of the driving seat 302 is fixed when injecting liquid each time, when the use position of the driving column 306 on the track column 3021 is not changed, the rotation angle of the driving gear 305 is also fixed, so that the downward moving distance of the piston block 202 is also fixed when pulling and pressing the seat 301 each time, that is, the injection amount of the container assembly each time is the same and fixed. When it is needed to adjust how much the single injection amount of the container assembly is, only the positioning screw 307 needs to be rotated. When the positioning screw 307 is rotated, the driving column 306 can be moved on the outside of the track column 3021 to change the use position through the rod body thread, so that the use position of the driving protrusion 3051 in the control groove 3061 when the control assembly 3 is in the reset state, that is, the effective range (height) of the driving protrusion 3051 entering the power groove 3062 after the driving seat 302 is completely moved down is changed. Because the driving protrusion 3051 will only drive the driving gear 305 to rotate after entering the inside of the power groove 3062, the change of the effective range of the driving protrusion 3051 entering the inside of the power groove 3062 will change the rotation angle of the driving gear 305, that is, the downward moving distance of the piston mechanism 2, so as to realize the adjustment and use of how much the single injection amount is, which can adapt to different types of microbial detection and analysis use, and has high adaptability.

[0041] The specific use and role of the embodiment are as follows: in the embodiment, the interior of the piston cavity 101 can extract the medicine liquid for standby, which can avoid frequent suction of the medicine liquid for standby during use, and is convenient to use. When it is necessary to reserve the medicine liquid for standby, the piston rod 201 is only needed to be lifted, the piston rod 201 can drive the piston block 202 to move upward synchronously and suck the medicine liquid into the interior of the piston cavity 101, and during the process, the piston rod 201 can reverse when being lifted under the cooperation of the linkage protrusion 2041 and the linkage groove 2011. The inner ring of the one-way ratchet 203 can rotate independently and will not jam the device when the piston rod 201 reverses, and the piston rod 201 will not drive the quantity control assembly 3 when being lifted, which guarantees the stable suction of the medicine liquid for standby. The injection operation of the container assembly can be realized through the quantity control assembly 3, and the injection amount of the container assembly is the same each time. The quantity control assembly 3 adopts instantaneous transmission control, which can effectively avoid the mis-discharge phenomenon caused by the press seat 301 not being pressed in place, and is stable and efficient to use. During the injection operation, the press seat 301 is only needed to be pulled down. Since the positioning block 304 is inserted into the interior of the positioning clamping groove 102 under the action of the positioning top spring 3031, the use position of the driving seat 302 is fixed, so the injection top spring 3012 can be compressed to store energy when the press seat 301 is pulled down. With the downward movement of the press seat 301, the trigger resistance rod 3011 can drive the trigger block 303 to move downward and compress the positioning top spring 3031 by abutting and extruding the trigger block 303. The control groove 3032 can drive the positioning block 304 to shrink into the interior of the driving seat 302 through the control rod 3041 when the trigger block 303 moves downward, so that the positioning block 304 is out of the interior of the positioning clamping groove 102, and the positioning function obtained by the driving seat 302 through the positioning block 304 is also invalid. Thereafter, under the action of the injection top spring 3012, the driving seat 302 can drive the driving column 306 to move downward and compress the reset top spring 3063 through the positioning screw 307, and complete the subsequent linkage injection action. When the driving column 306 moves downward, the driving protrusion 3051 enters the interior of the power groove 3062 from the regulation groove 3061, and the driving protrusion 3051 does not drive the driving gear 305 to rotate when moving in the regulation groove 3061. After the driving protrusion 3051 enters the interior of the power groove 3062, the driving gear 305 also rotates with the downward movement of the driving column 306. The driving gear 305 can drive the linkage gear 204 to rotate when rotating. The linkage protrusion 2041 can drive the piston rod 201 to move downward through the linkage groove 2011 when the linkage gear 204 rotates. The piston rod 201 can drive the piston block 202 to move downward synchronously, so that the medicine liquid in the interior of the piston cavity 101 is discharged. The injection is completed once,After the press seat 301 is loosened, under the action of the reset top spring 3063, the control assembly 3 can be reset as a whole to move upward, ready for subsequent liquid injection use, and in the process of upward reset, the linkage gear 204 will drive the piston rod 201 to reverse (i.e. the inner circle of the one-way ratchet 203 idles), and will not drive the piston block 202 to move upward. Since the movement distance of the drive seat 302 is fixed during each injection, when the use position of the drive column 306 on the track column 3021 is not changed, the rotation angle of the drive gear 305 is also fixed, so that the downward movement distance of the piston block 202 is also fixed when the press seat 301 is pulled each time, that is, the injection amount of the container assembly each time is also the same and fixed. When it is needed to adjust how much the single injection amount of the container assembly is, only the positioning screw 307 needs to be turned, when the positioning screw 307 is turned, it can drive the drive column 306 to move outside the track column 3021 to change the use position, so that the drive protrusion 3051 in the control assembly 3 in the reset state is inside the use position of the control groove 3061, that is, the effective range (height) of the drive protrusion 3051 entering the power groove 3062 inside after the drive seat 302 is completely moved downward. Because only when the drive protrusion 3051 enters the inside of the power groove 3062, the drive gear 305 will be driven to rotate, and the change of the effective range of the drive protrusion 3051 entering the inside of the power groove 3062 will change the rotation angle of the drive gear 305, that is, the downward movement distance of the piston mechanism 2, so as to realize the adjustment and use of how much the single injection amount is, which can adapt to different types of microbial detection and analysis use.

Claims

1. A microbial detection and analysis device, characterized in that, include: The container assembly includes a medicine reservoir (1) and a piston mechanism (2). The piston mechanism (2) includes a piston rod (201), a piston block (202), a one-way ratchet (203), and a linkage gear (204). The piston rod (201) is inserted into the top of the medicine reservoir (1), and the piston block (202) is inserted into the piston cavity (101) of the medicine reservoir (1). The outer ring of the one-way ratchet (203) is fixedly installed on the top of the piston block (202), and the inner ring of the one-way ratchet (203) is fixedly installed on the bottom of the piston rod (201). The linkage gear (204) is rotatably connected to the inside of the medicine reservoir (1). The volume control assembly (3) includes a pressing seat (301), a driving seat (302), a trigger block (303), a positioning block (304), and a driving gear. Wheel (305), drive column (306) and positioning screw (307), press seat (301), drive seat (302) and drive column (306) are inserted into the medicine storage seat (1) from top to bottom, and drive seat (302) is inserted into the bottom of press seat (301), trigger block (303) is inserted into the inside of drive seat (302), and positioning block (304) is inserted into the inside of drive seat (302), drive gear (305) is rotatably connected to the inside of medicine storage seat (1), and drive gear (305) and linkage gear (204) mesh and drive, drive column (306) is inserted into the bottom of drive seat (302), positioning screw (307) is rotatably connected to the inside of drive column (306), and positioning screw (307) is screwed into the bottom of drive seat (302) through rod thread; The bottom of the pressing seat (301) is provided with a trigger rod (3011), and the trigger rod (3011) is located directly above the trigger block (303). The bottom of the pressing seat (301) is provided with an injection top spring (3012), and the two ends of the injection top spring (3012) abut against the bottom of the pressing seat (301) and the top of the drive seat (302) respectively. The bottom of the trigger block (303) is provided with a positioning top spring (3031), and the two ends of the positioning top spring (3031) abut against the inside of the trigger block (303) and the inside of the drive seat (302), respectively; The trigger block (303) has an inclined control groove (3032) inside, and the positioning block (304) has a control rod (3041) inside. The control rod (3041) is inserted into the control groove (3032). The medicine storage seat (1) has a positioning slot (102) inside, and the positioning block (304) is inserted into the positioning slot (102). The drive column (306) has a drive groove on its outside and a drive protrusion (3051) is provided inside the drive gear (305), and the drive protrusion (3051) is inserted into the inside of the drive groove. The drive groove consists of an adjustment groove (3061) arranged along the axial direction of the drive column (306) and a power groove (3062) arranged spirally, with the bottom end of the power groove (3062) connected to the top end of the adjustment groove (3061).

2. The microbial detection and analysis device according to claim 1, characterized in that, The piston rod (201) has a spiral-oriented linkage groove (2011) on its outside, and the linkage gear (204) has a linkage protrusion (2041) inside, which is inserted into the linkage groove (2011).

3. The microbial detection and analysis device according to claim 2, characterized in that, The piston block (202) can only move vertically inside the piston chamber (101), and the bottom of the drive seat (302) is provided with a track post (3021) with a regular polygonal cross section, and the track post (3021) is inserted into the drive post (306).

4. The microbial detection and analysis device according to claim 3, characterized in that, The bottom of the drive column (306) is provided with a reset top spring (3063), and the two ends of the reset top spring (3063) abut against the bottom of the drive column (306) and the inside of the medicine reservoir (1) respectively, and the elastic force of the reset top spring (3063) is less than the elastic force of the injection top spring (3012).

Images