Microorganism fluorescence rapid detector and detection method

Abstract

The invention discloses a rapid microbial fluorescence detector and a detection method, and relates to the technical field of microbial fluorescence detection.The rapid microbial fluorescence detector comprises a detector shell, a top cover is connected to the top of the detector shell in a covering mode, a detection mechanism is fixedly installed in the detector shell, and an auxiliary mechanism is fixedly connected to one side of the detection mechanism; a containing frame is fixedly installed on the inner side wall of the detector shell, and the detection mechanism comprises an incubation cylinder. Through cooperative operation of an electric heating block, a heat insulation layer, a micro motor and a partition plate, mixing and heating of materials are accelerated, the interior of an incubation barrel is divided into four parts by the partition plate, in combination with auxiliary heating of a resistance block, the incubation temperature can rapidly reach a suitable range, the incubation time is greatly shortened, and compared with detection of escherichia coli in food, the method has the advantages that the efficiency is high; detection can be completed in a short time, the detection efficiency is improved, and the requirements of food production, medical diagnosis, environment monitoring and other fields for rapid detection results are met in time.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fluorescence detection, and in particular to a microbial fluorescence rapid detector and a detection method. Background Art

[0002] Microbial fluorescence detection is a method of detecting microorganisms by utilizing the fluorescence properties of microorganisms themselves or with the help of fluorescent labeling technology, and plays a key role in many fields.

[0003] However, in the existing technology, traditional microbial detection methods, such as culture methods, although they have high accuracy, have a lengthy detection cycle, usually taking days or even weeks to obtain results, which is difficult to meet the urgent demand for rapid detection in modern society. For example, in food processing companies, if the microbial contamination in food cannot be detected in time, it may cause a large amount of contaminated food to flow into the market, endangering the health of consumers; in hospital infection prevention and control scenarios, the inability to quickly identify pathogens will delay the treatment of patients. Some existing rapid detection technologies also have many shortcomings. Some rapid detection equipment has a complex structure and cumbersome operation procedures, and requires professional technicians to operate and maintain it, which limits its application in grassroots units or on-site testing; the detection accuracy of some equipment is easily affected by external environmental factors. For example, changes in temperature and humidity may cause deviations in test results and result in misjudgment. Summary of the Invention

[0004] The purpose of the present invention is to provide a microbial fluorescence rapid detector and detection method to solve the problems of the existing microbial detection technology mentioned in the above background technology, such as long detection cycle, complex operation, and accuracy greatly affected by external factors.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solutions: a microbial fluorescence rapid detector and detection method, comprising a detector housing, a top cover connected to the top cover of the detector housing, a detection mechanism fixedly installed inside the detector housing, an auxiliary mechanism fixedly connected to one side of the detection mechanism, and a receiving frame fixedly installed on the inner side wall of the detector housing; The detection mechanism includes an incubation cylinder, the top of which is connected to a sample injection pipe, one end of which is connected to a bent pipe, the end of which is located on the axis of the incubation cylinder, and auxiliary pipes are arranged at equal intervals around the sample injection pipe, and the ends of the auxiliary pipes are arranged in a curved state toward the detector housing; The auxiliary mechanism includes an electric heating block, an insulation layer is fixedly connected to one side of the electric heating block shell, micro motors are fixedly installed at equal intervals inside the insulation layer, and the output end of the micro motor is fixedly connected to a partition plate through a coupling. The partition plate is horizontally arranged inside the incubation cylinder, and the partition plate divides the internal space of the incubation cylinder into four equal parts.

[0006] The other end of the sample injection pipe is connected to a pretreatment chamber, which is fixedly mounted on the inner wall of the detector housing. A detection tray is fixedly mounted on the bottom of the detector housing, and the detection tray is located below the incubation cylinder.

[0007] Preferably, the bottom of the incubation cylinder is connected to a vertical pipe, the vertical pipe is connected to the detection tray, and a first solenoid valve is fixedly installed on the pipe body of the vertical pipe, and the first solenoid valve is used to control the falling of the material.

[0008] Preferably, storage cylinders are provided on both sides of the incubation cylinder, and multiple storage cylinders are arranged linearly and vertically on the inner side of the detector housing. A positioning frame is clamped on one side of the storage cylinder, and a pressing block is inserted on the top of the positioning frame, and the pressing block is overlapped on the topmost storage cylinder.

[0009] Preferably, a partition is fixedly installed on the positioning frame between two adjacent storage cylinders, and a support base is fixedly installed on the bottom of the positioning frame, and the support base is fixedly connected to the detection disk and the detector housing at the same time.

[0010] Preferably, a micro pump is provided inside each storage cylinder, and the water outlet of the micro pump is connected to a delivery hose, which is connected to the secondary pipeline.

[0011] Preferably, a protection frame is fixedly connected to the outer wall of the incubation cylinder, a rear extension rod is fixedly installed on the bottom of the protection frame, the extension rod is fixedly connected to the vertical pipe, and the protection frame is fixedly connected to the positioning frame.

[0012] Preferably, the top of the incubation cylinder is connected to a pressure relief valve, which is located on the axis of the incubation cylinder. One side of the pressure relief valve is fixedly connected to a second solenoid valve, which is fixedly installed on the top of the protective frame.

[0013] Preferably, support rods are fixedly connected between the multiple micro motors, the support rods are fixedly connected to the insulation layer, an extension wire is fixedly connected to the electric heating block, a resistance block is provided inside the partition plate, and the extension wire is connected to the resistance block.

[0014] A detection method for a microbial fluorescence rapid detector comprises the following steps: Sample pretreatment: Place the sample in the pretreatment chamber and inject it into the central axis of the incubation tube through the sample injection pipe and the bent pipe. At the same time, the micro pump in the storage tube is started, and auxiliary materials such as reaction reagents and diluents are added to the incubation tube through the delivery hose and auxiliary pipe; Sample incubation: The electric heating block works, and heat is transferred through the insulation layer. The micro motor drives the partition plate to rotate, which divides the interior of the incubation cylinder into four parts, accelerating the mixing and heating of the materials. At the same time, the resistor block in the partition plate assists in heating. During the incubation process, the internal pressure is adjusted by the pressure relief valve and the second solenoid valve. Sample detection: After the incubation is completed, the first solenoid valve is opened, and the sample falls into the detection plate through the vertical pipe. The detector performs fluorescence detection and analysis on the sample on the detection plate to obtain the test results; Post-test processing: After the test is completed, the incubation cylinder and other components can be cleaned to prepare for the next test. At the same time, the pressing block can be removed and the material in the storage cylinder can be replaced.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. In the present invention, the coordinated operation of the electric heating block, the insulation layer, the micro motor and the partition plate accelerates the mixing and heating of the materials. The partition plate divides the interior of the incubation cylinder into four parts. Combined with the auxiliary heating of the resistance block, the incubation temperature can be quickly brought to an appropriate range, greatly shortening the incubation time. For example, compared with traditional methods, the detection of E. coli in food can be completed in a short time, thereby improving the detection efficiency and promptly meeting the needs of food production, medical diagnosis, environmental monitoring and other fields for rapid detection results.

[0016] 2. In the present invention, the positioning frame, pressing blocks, and support base provide stability and protection for the storage cylinder. The protective frame is connected to the incubation cylinder, vertical pipe, and positioning frame, enhancing overall structural stability. The storage frame can accommodate components such as batteries, making rational use of space, making the entire device more reliable during operation, reducing detection errors caused by loose components and displacement, and extending the device's service life.

[0017] 3. In the present invention, the detection mechanism is set up to fully mix the sample and auxiliary materials. The pretreatment chamber performs preliminary treatment on the sample to remove impurities, adjust the concentration, and reduce interference factors. During the detection process, the incubation temperature, pressure and other conditions are precisely controlled. In addition, the fluorescence detection technology itself has high sensitivity and specificity. The combined effect of multiple factors ensures that the test results can accurately reflect the types and quantities of microorganisms in the sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the three-dimensional structure of a microbial fluorescence rapid detector and detection method of the present invention; Figure 2 This is a top view of the interior of the detector housing of a microbial fluorescence rapid detector and detection method of the present invention; Figure 3 This is a schematic diagram of the internal three-dimensional structure of the detector housing of a microbial fluorescence rapid detector and detection method of the present invention; Figure 4 For the present invention Figure 3 A schematic diagram of the structure of part A; Figure 5 This is a schematic diagram of the planar structure of the detection mechanism and auxiliary mechanism of a microbial fluorescence rapid detection instrument and detection method of the present invention; Figure 6 This is a schematic diagram of the three-dimensional structure of the detection mechanism and auxiliary mechanism of a microbial fluorescence rapid detection instrument and detection method of the present invention; Figure 7 This is a schematic diagram of the internal structure of an incubation tube of a microbial fluorescence rapid detector and detection method of the present invention; Figure 8 This is a schematic diagram of the connection structure of the electric heating block and extension wire of a microbial fluorescence rapid detector and detection method of the present invention.

[0019] In the figure: 1. Detector housing; 2. Detection mechanism; 3. Receiving frame; 4. Top cover; 5. Auxiliary mechanism; 6. Positioning frame; 7. Storage cylinder; 8. Pressing block; 9. Support base; 10. Detection tray; 21. Pretreatment chamber; 22. Sample injection pipeline; 23. Protection frame; 24. Auxiliary pipeline; 25. Incubation cylinder; 26. Extension rod; 27. Delivery hose; 28. Vertical pipeline; 29. First solenoid valve; 210. Pressure relief valve; 211. Second solenoid valve; 212. Bent pipeline; 51. Electric heating block; 52. Insulation layer; 53. Partition plate; 54. Micro motor; 55. Support rod; 56. Extension wire. DETAILED DESCRIPTION

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the implementation regulations described are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0021] Example 1: Reference Figure 1 、 Figure 2 、 Figure 3 、 Figure 5 、 Figure 6 and Figure 7As shown: it includes a detector housing 1, the top cover of the detector housing 1 is connected to a top cover 4, a detection mechanism 2 is fixedly installed inside the detector housing 1, one side of the detection mechanism 2 is fixedly connected to an auxiliary mechanism 5, a receiving frame 3 is fixedly installed on the inner side wall of the detector housing 1, the detection mechanism 2 includes an incubation cylinder 25, the top of the incubation cylinder 25 is connected to a sample injection pipe 22, one end of the sample injection pipe 22 is connected to a bent pipe 212, the end of the bent pipe 212 is located on the axis of the incubation cylinder 25, and the sample injection pipe 2 2 is provided with auxiliary pipes 24 at equal intervals around the incubation tube 25, and the ends of the auxiliary pipes 24 are provided in a bent state toward the detector housing 1, the auxiliary mechanism 5 includes an electric heating block 51, and a heat insulating layer 52 is fixedly connected to one side of the outer shell of the electric heating block 51, and micro motors 54 are fixedly installed at equal intervals inside the heat insulating layer 52, and the output end of the micro motor 54 is fixedly connected to a partition plate 53 through a coupling, and the partition plate 53 is horizontally provided inside the incubation tube 25, and the partition plate 53 divides the internal space of the incubation tube 25 into four equal parts.

[0022] In this embodiment, when conducting food microbiological detection, the collected food samples are first placed in the pretreatment chamber 21. For solid food samples, such as meat, bread, etc., they are first crushed to make them into uniform fine particles, and then an appropriate amount of physiological saline or buffer solution is added to dissolve them to make a sample solution suitable for detection. For liquid food samples, such as milk, beverages, etc., they are filtered to remove large particles of impurities, and then appropriately diluted or concentrated according to the initial concentration of the sample. The treated sample is injected into the central axis position of the incubation tube 25 through the sample injection pipe 22 and the bent pipe 212. At the same time, the reaction reagents such as fluorescent markers, enzymes, etc. and diluents pre-stored in the storage tube 7 are injected into the incubation tube 25 through the delivery hose 27 and the auxiliary pipe 24 under the action of the micro pump and are fully mixed with the sample.

[0023] Then the electric heating block 51 is started, and the heat generated by the electric heating block 51 is transferred to the incubation tube 25 through the insulation layer 52, wherein the micro motor 54 drives the partition plate 53 to rotate to a horizontal state, and the partition plate 53 divides the interior of the incubation tube 25 into four parts, three of which are used to accelerate the mixing and heating of the materials, and the other one is used for unloading. The resistor block in the partition plate 53 is assisted in heating under the action of the electric heating block 51 to ensure that the incubation temperature is maintained within a range suitable for microbial reaction. The resistance value of the resistor in each partition plate 53 is different, and is used to correspond to a temperature respectively. For example, for common E. coli detection, the incubation temperature is controlled at about 37°C. During the incubation process, if the pressure in the incubation tube 25 increases, the second solenoid valve 211 controls the pressure relief valve 210 to open, release the pressure, and ensure a stable incubation environment.

[0024] After the incubation is completed, the partition plate 53 is rotated again and the first solenoid valve 29 is opened. The sample falls into the detection tray 10 through the vertical pipe 28. The detector performs fluorescence detection and analysis on the sample on the detection tray 10. According to the intensity and characteristics of the fluorescence signal, the detection results such as the type and number of microorganisms in the food sample are obtained. For example, by comparing with the standard fluorescence intensity curve, it is determined whether the content of Escherichia coli in the food exceeds the standard.

[0025] After the test is completed, turn off the power of the instrument, clean the incubation tube 25, sample injection pipe 22, vertical pipe 28 and other components to remove residual samples and reagents. Use a special cleaning agent and distilled water to rinse multiple times to ensure that the inside of the instrument is clean. Remove the pressing block 8, replace the reaction reagent and diluent in the storage tube 7, and prepare for the next test.

[0026] When conducting microbial testing in the medical field, such as detecting pathogens in a patient's sputum sample, the operating steps are as follows: first, the collected sputum sample is placed in the pretreatment chamber 21, and an appropriate amount of a liquefier, such as N-acetylcysteine, is added to liquefy the sputum to make it thinner and facilitate subsequent testing operations. The treated sample is then injected into the central axis of the incubation tube 25 through the sample injection pipe 22 and the curved pipe 212. At the same time, the micropump in the storage tube 7 is started, and the corresponding reaction reagents, such as a fluorescent probe for specific pathogens and a diluent, are injected into the incubation tube 25 through the delivery hose 27 and the auxiliary pipe 24.

[0027] The subsequent incubation, detection and post-detection processing steps are basically the same as the previous scheme. During the incubation process, the appropriate incubation temperature and time are set according to the characteristics of the target pathogens. For example, when detecting Streptococcus pneumoniae, the incubation temperature can be set to 35-37°C, and the incubation time is adjusted according to the actual situation. The detector and detection method of the present invention can quickly detect the type and number of pathogens in sputum samples, providing an important basis for doctors' clinical diagnosis and treatment. After the test is completed, the instrument is cleaned and maintained, and the material in the storage cylinder 7 is replaced to ensure the accuracy and reliability of the instrument for the next use.

[0028] When conducting microbial detection of environmental water samples, take the detection of river water samples as an example: first put the collected water sample into the pretreatment chamber 21. If there are many impurities in the water sample, use a filtering device such as a 0.45μm filter membrane to filter and remove large particle impurities. If the microbial concentration is too low, use concentration technology such as centrifugal concentration or membrane filtration concentration for enrichment. The treated water sample is injected into the central axis position of the incubation tube 25 through the sample injection pipe 22 and the bent pipe 212. At the same time, the micro pump in the storage tube 7 is started to inject the reaction reagent and diluent into the incubation tube 25.

[0029] Subsequently, incubation, testing and post-test processing are carried out according to the procedures set by the instrument. During the incubation process, the incubation conditions are reasonably set according to the characteristics of common microorganisms in environmental water samples. After the test is completed, the microbial contamination status of the river water sample is evaluated based on the test results to provide data support for environmental monitoring and water resource protection. At the same time, the instrument is routinely cleaned and maintained, and the material in the storage cylinder 7 is replaced to ensure that the instrument is ready for the next test at any time.

[0030] Example 2: Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 and Figure 8 As shown, the detection mechanism 2 includes an incubation cylinder 25, the top of the incubation cylinder 25 is connected to a sample injection pipe 22, one end of the sample injection pipe 22 is connected to a bent pipe 212, the end of the bent pipe 212 is located on the axis of the incubation cylinder 25, and auxiliary pipes 24 are arranged at equal intervals around the sample injection pipe 22, and the end of the auxiliary pipe 24 is set to a bent state facing the detector housing 1, the other end of the sample injection pipe 22 is connected to a pretreatment chamber 21, the pretreatment chamber 21 is fixedly mounted on the inner wall of the detector housing 1, the bottom of the detector housing 1 is fixedly mounted with a detection disk 10, the detection disk 10 is located below the incubation cylinder 25, the bottom of the incubation cylinder 25 is connected to a vertical pipe 28, the vertical pipe 28 is connected to the detection disk 10, and the first solenoid valve 2 is fixedly mounted on the tube body of the vertical pipe 28 9. The first solenoid valve 29 is used to control the falling of materials. A protective frame 23 is fixedly connected to the outer wall of the incubation cylinder 25. A rear extension rod 26 is fixedly installed on the bottom of the protective frame 23. The extension rod 26 is fixedly connected to the vertical pipe 28. The protective frame 23 is fixedly connected to the positioning frame 6. The top of the incubation cylinder 25 is connected to a pressure relief valve 210. The pressure relief valve 210 is located on the axis of the incubation cylinder 25. A second solenoid valve 211 is fixedly connected to one side of the pressure relief valve 210. The second solenoid valve 211 is fixedly installed on the top of the protective frame 23. A support rod 55 is fixedly connected between the multiple micro motors 54. The support rod 55 is fixedly connected to the insulation layer 52. An extension wire 56 is fixedly connected to the electric heating block 51. A resistance block is provided inside the partition plate 53, and the extension wire 56 is connected to the resistance block.

[0031] In this embodiment, the instrument structure and component functions are as follows: the detection mechanism 2, the auxiliary mechanism 5, and the accommodating frame 3 are integrated inside the detector housing 1. The accommodating frame 3 is used to place batteries to provide power for the auxiliary mechanism 5. The core of the detection mechanism 2 is an incubation tube 25. The top sample injection pipe 22 is connected to the bending pipe 212 to accurately inject the sample into the center axis of the tube. The surrounding auxiliary pipes 24 can add auxiliary materials. The pretreatment chamber 21 processes the sample and then transports it through the sample injection pipe 22. The vertical pipe 28 at the bottom of the incubation tube 25 is connected to the detection plate 10. The sample is controlled to fall by the first solenoid valve 29. The protective frame 23 protects the incubation tube 25 and is connected to the vertical pipe 28 through the extension rod 26 and fixed to the positioning frame 6. The positioning frame 6 stabilizes the storage tube 7. The pressure relief valve 210 and the second solenoid valve 211 at the top of the incubation tube 25 adjust the internal pressure.

[0032] Detection process: The sample is placed in the pretreatment chamber 21 for treatment, such as removing impurities, adjusting concentration, etc. The treated sample is injected into the central axis position of the incubation tube 25 through the sample injection pipe 22 and the bent pipe 212. At the same time, the micro pump in the storage tube 7 is started, and reaction reagents, diluents and other auxiliary materials are added to the incubation tube 25 through the delivery hose 27 and the auxiliary pipe 24 to make them fully mixed. The electric heating block 51 works, and heat is transferred through the insulation layer 52. The micro motor 54 drives the partition plate 53 to rotate, and the resistance block in the partition plate 53 assists in heating to ensure that the incubation temperature is appropriate. During the incubation process, if the internal pressure is too high, the second solenoid valve 211 controls the pressure relief valve 210 to open and relieve pressure. After the incubation is completed, the first solenoid valve 29 is opened, and the sample falls into the detection disk 10 through the vertical pipe 28. The detector performs fluorescence detection and analysis on the sample on the detection disk 10 to obtain the detection result.

[0033] Example 3: Figure 2 and Figure 3 As shown, storage cylinders 7 are provided on both sides of the incubation cylinder 25, and multiple storage cylinders 7 are linearly and vertically arranged on the inner side of the detector housing 1. A positioning frame 6 is clamped on one side of the storage cylinder 7, and a pressing block 8 is inserted on the top of the positioning frame 6. The pressing block 8 is overlapped on the topmost storage cylinder 7. A partition is fixedly installed between two adjacent storage cylinders 7 on the positioning frame 6, and a support base 9 is fixedly installed on the bottom of the positioning frame 6. The support base 9 is fixedly connected to the detection disk 10 and the detector housing 1 at the same time. A micro pump is provided inside each storage cylinder 7, and the water outlet of the micro pump is connected to a delivery hose 27, and the delivery hose 27 is connected to the auxiliary pipeline 24.

[0034] In this embodiment, the equipment structure is installed and fixed: multiple storage cylinders 7 are linearly and vertically arranged on the inner side of the detector housing 1, and one side of the storage cylinder 7 is clamped onto the positioning frame 6. The positioning frame 6 is provided with partitions between adjacent storage cylinders 7 to prevent mutual contamination of reagents. The support base 9 is fixed to the bottom of the positioning frame 6 so that the support base 9 is firmly connected to the detection disk 10 and the detector housing 1 at the same time to enhance the stability of the overall structure. The pressing block 8 is inserted into the top of the positioning frame 6 and overlapped on the topmost storage cylinder 7 to further fix the storage cylinder 7.

[0035] Reagent storage and preparation: Reaction reagents, diluents and other materials required for detection are loaded into each storage cylinder 7. A micro pump is installed inside the storage cylinder 7 to provide power for reagent transportation.

[0036] The reagent is transported to the reaction area: during detection, the micro pump in the storage cylinder 7 is started, and the micro pump pumps the reagent in the storage cylinder 7 into the delivery hose 27 through the water outlet. The delivery hose 27 is connected to the auxiliary pipe 24 to transport the reagent to the auxiliary pipe 24. The auxiliary pipe 24 surrounds the sample injection pipe 22, and the end is bent toward the detector housing 1, so that the reagent can be better diffused in the incubation cylinder 25 and fully mixed with the sample entering the incubation cylinder 25 through the sample injection pipe 22, preparing for subsequent incubation detection.

[0037] The method of use and working principle of this device are as follows: when conducting food microbiological testing, the collected sample is first placed in the pretreatment chamber 21. For solid samples, it may be necessary to first perform processing such as crushing and dissolving. For liquid samples, it is necessary to perform filtering and concentration adjustment. The processed sample is injected into the central axis position of the incubation cylinder 25 through the sample injection pipe 22 and the curved pipe 212. At the same time, the reaction reagent and diluent pre-stored in the storage cylinder 7 are injected into the incubation cylinder 25 through the delivery hose 27 and the auxiliary pipe 24 under the action of the micro pump to be fully mixed with the sample. Then the electric heating block 51 is started. The heat generated by the electric heating block 51 is transferred to the incubation cylinder 25 through the heat insulation layer 52. The micro motor 54 drives the partition plate 53 to start rotating. The partition plate 53 divides the interior of the incubation cylinder 25 into four parts, three of which are used to accelerate the mixing and heating of the materials. The resistor block in the partition plate 53 is assisted by heating under the action of the electric heating block 51 to ensure that the incubation temperature is maintained within a range suitable for microbial reaction. During the incubation process, if the pressure in the incubation cylinder 25 increases, the second solenoid valve 211 controls the pressure relief valve 210 to open, release the pressure, and ensure a stable incubation environment. After the incubation is completed, the first solenoid valve 29 is opened, and the sample falls into the test tray 10 through the vertical pipe 28. The detector performs fluorescence detection and analysis on the sample on the test tray 10. Based on the intensity and characteristics of the fluorescence signal, the test results such as the type and number of microorganisms in the food sample are obtained; After the test is completed, turn off the power of the instrument, clean the incubation tube 25, sample injection pipe 22, vertical pipe 28 and other components, remove the residual sample and reagent, remove the pressing block 8, replace the reaction reagent and diluent in the storage tube 7, and prepare for the next test.

[0038] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A microbial fluorescence rapid detector, comprising a detector housing (1), the top cover of the detector housing (1) being connected to a top cover (4), characterized in that: A detection mechanism (2) is fixedly installed inside the detector housing (1), an auxiliary mechanism (5) is fixedly connected to one side of the detection mechanism (2), and a receiving frame (3) is fixedly installed on the inner side wall of the detector housing (1); The detection mechanism (2) includes an incubation tube (25), the top of the incubation tube (25) is connected to a sample injection pipe (22), one end of the sample injection pipe (22) is connected to a bent pipe (212), the end of the bent pipe (212) is located on the axis of the incubation tube (25), and auxiliary pipes (24) are arranged at equal intervals around the sample injection pipe (22), and the end of the auxiliary pipe (24) is arranged in a bent state toward the detector housing (1); The auxiliary mechanism (5) includes an electric heating block (51), a heat insulating layer (52) is fixedly connected to one side of the outer shell of the electric heating block (51), micro motors (54) are fixedly installed at equal intervals inside the heat insulating layer (52), and the output end of the micro motor (54) is fixedly connected to a partition plate (53) through a coupling. The partition plate (53) is horizontally arranged inside the incubation cylinder (25), and the partition plate (53) divides the internal space of the incubation cylinder (25) into four equal parts.

2. A microbial fluorescence rapid detector according to claim 1, characterized in that: The other end of the sample injection pipe (22) is connected to the pretreatment chamber (21), which is fixedly mounted on the inner wall of the detector housing (1). A detection tray (10) is fixedly mounted on the bottom of the detector housing (1), and the detection tray (10) is located below the incubation cylinder (25).

3. A microbial fluorescence rapid detector according to claim 2, characterized in that: The bottom of the incubation cylinder (25) is connected to a vertical pipe (28), which is connected to the detection plate (10). A first solenoid valve (29) is fixedly installed on the pipe body of the vertical pipe (28), and the first solenoid valve (29) is used to control the falling of the material.

4. A microbial fluorescence rapid detector according to claim 3, characterized in that: Storage cylinders (7) are provided on both sides of the incubation cylinder (25), and a plurality of storage cylinders (7) are arranged linearly and vertically on the inner side of the detector housing (1). A positioning frame (6) is clamped on one side of the storage cylinder (7), and a pressing block (8) is inserted on the top of the positioning frame (6). The pressing block (8) is overlapped on the topmost storage cylinder (7).

5. A microbial fluorescence rapid detector according to claim 4, characterized in that: The positioning frame (6) is fixedly mounted with a partition between two adjacent storage cylinders (7), and a support base (9) is fixedly mounted on the bottom of the positioning frame (6). The support base (9) is fixedly connected to the detection disk (10) and the detector housing (1).

6. A microbial fluorescence rapid detector according to claim 5, characterized in that: A micro pump is provided inside each storage cylinder (7), and the water outlet of the micro pump is connected to a delivery hose (27), which is in communication with the auxiliary pipeline (24).

7. A microbial fluorescence rapid detector according to claim 6, characterized in that: A protective frame (23) is fixedly connected to the outer wall of the incubation cylinder (25), an extension rod (26) is fixedly installed at the bottom of the protective frame (23), the extension rod (26) is fixedly connected to the vertical pipe (28), and the protective frame (23) is fixedly connected to the positioning frame (6).

8. A microbial fluorescence rapid detector according to claim 7, characterized in that: The top of the incubation cylinder (25) is connected to a pressure relief valve (210), which is located on the axis of the incubation cylinder (25). One side of the pressure relief valve (210) is fixedly connected to a second solenoid valve (211), which is fixedly installed on the top of the protection frame (23).

9. A microbial fluorescence rapid detector according to claim 8, characterized in that: A support rod (55) is fixedly connected between the plurality of micro motors (54), the support rod (55) is fixedly connected to the heat insulation layer (52), an extension wire (56) is fixedly connected to the electric heating block (51), a resistor block is provided inside the partition plate (53), and the extension wire (56) is connected to the resistor block.

10. A detection method using a microbial fluorescence rapid detector, using a microbial fluorescence rapid detector according to any one of claims 1 to 9, characterized in that: The following steps are involved: Sample pretreatment: The sample is placed in the pretreatment chamber (21), and injected into the central axis of the incubation cylinder (25) through the sample injection pipe (22) and the bent pipe (212). At the same time, the micro pump in the storage cylinder (7) is started, and the reaction reagent and diluent auxiliary materials are added to the incubation cylinder (25) through the delivery hose (27) and the auxiliary pipe (24); Sample incubation: The electric heating block (51) works, and heat is transferred through the heat insulation layer (52). The micro motor (54) drives the partition plate (53) to rotate. The partition plate (53) divides the interior of the incubation tube (25) into four parts, accelerating the mixing and heating of the materials. At the same time, the resistance block in the partition plate (53) assists in heating. During the incubation process, the internal pressure is adjusted by the pressure relief valve (210) and the second solenoid valve (211); Sample detection: After the incubation is completed, the first electromagnetic valve (29) is opened, and the sample falls into the detection plate (10) through the vertical pipe (28). The detector performs fluorescence detection and analysis on the sample on the detection plate (10) to obtain the detection result; Post-test processing: After the test is completed, the incubation cylinder (25) and other components can be cleaned to prepare for the next test. At the same time, the pressing block (8) can be removed and the material in the storage cylinder (7) can be replaced.

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