A rhizosphere soil sampling device and method
By designing a visual rhizosphere soil sampling device and using a camera module, LED light group and liquid supply and suction system, accurate sampling of rhizosphere soil is achieved, which solves the problem of root damage in existing technologies and improves sampling accuracy and efficiency. It is suitable for agricultural science, plant ecology and environmental monitoring.
Patent Information
- Application Number
- CN202510670665.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-23
- Publication Date
- 2025-10-17
- Estimated Expiration
- 2045-05-23
AI Technical Summary
In the existing technology, conventional sampling tools are prone to damaging plant roots during the sampling process, causing plant damage, making in-situ observation or long-term continuous sampling impossible, and the sampling accuracy and efficiency are insufficient.
An integrated visual, non-destructive rhizosphere soil sampling device was designed, including an operating handle, a sampling tube and an end head. The sampling tube is integrated with an optical wire assembly, a liquid supply tube, a liquid suction tube and a steel wire rope. The end head is equipped with a camera module, an LED light group, a liquid outlet hole and a liquid suction hole. Real-time observation and precise control are achieved through the display screen and button assembly. The snake tube is flexible and plastic, and the liquid supply and liquid suction systems are used in conjunction to collect soil samples.
It realizes the visualization and precise sampling of rhizosphere soil, avoids damage to the root system, improves sampling accuracy and efficiency, and is suitable for fields such as agricultural science, plant ecology and environmental monitoring.
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Figure CN120489636B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the field of soil sampling, and relates to a device and method for rhizosphere soil sampling, which is particularly suitable for the scene of non-destructive in-situ sampling of rhizosphere soil in the fields of agricultural science, plant ecology, environmental monitoring and the like. BACKGROUND
[0002] Soil sampling is a basic work for studying plant-soil interaction, soil fertility evaluation and environmental pollution monitoring. Rhizosphere soil, as a micro-environment directly contacted by plant roots and soil, has an important influence on plant growth due to its physicochemical properties and microbial activities.
[0003] In the prior art, conventional sampling tools (such as soil drills, spades and the like) need to dig the soil and cut the roots, resulting in damage to the plants and failure to achieve in-situ observation or long-term continuous sampling. For example, mechanical drills are prone to cutting off plant roots during operation, damaging the rhizosphere micro-environment and affecting the accuracy of subsequent research. SUMMARY
[0004] In view of this, the present application aims to provide a rhizosphere soil sampling device integrated with visualization and non-destructiveness, so as to solve the defects of damage to roots, low sampling accuracy and insufficient operation efficiency of the conventional technology.
[0005] The present application provides a rhizosphere soil sampling device, which comprises:
[0006] An operating handle, which is provided with a display screen, a switch button assembly and a direction operating lever;
[0007] A sampling tube, which comprises a snake bone tube and a tip, and is integrated with a photoelectric wire group, a liquid supply pipe, a liquid suction pipe and a plurality of steel wire ropes; the tip has a spherical surface at the front end, and an L-shaped notch is formed in the surface of the spherical surface, the L-shaped notch has a first panel and a second panel, wherein:
[0008] The first panel has an included angle of 170°-180° with the direction of the tip axis, and is integrated with a camera module thereon, a LED lamp group is symmetrically arranged on both sides of the camera module, and a liquid outlet hole is arranged on the front side and connected with the liquid supply pipe; the lens surface of the camera module is embedded in the first panel to avoid direct contact or extrusion of soil;
[0009] The second panel is perpendicular to the direction of the tip axis, and a liquid suction hole is arranged on the second panel and connected with the liquid suction pipe;
[0010] The snake bone tube comprises a plurality of joints which are sequentially hinged, and the bending is realized by controlling the winding and unwinding of the steel wire ropes through the direction operating lever.
[0011] Compared with the prior art, the application has the beneficial effects that:
[0012] The device can realize visualization and accurate sampling of rhizosphere soil, avoid damage to root systems, and improve sampling accuracy and efficiency. First, through the display screen and switch button assembly on the handle, the user can observe the image and data in real time during the sampling process and conveniently control various functions of the device, such as lighting, photographing, image zooming, and sample collection, to realize accurate control of the sampling process. Second, the snake bone tube and end head in the sampling tube are designed uniquely, the multiple articulated joints of the snake bone tube have good flexibility and plasticity, and can adapt to different soil environments and root system distribution, while the camera module, LED lamp group, liquid outlet hole, and liquid suction hole on the end head realize visualization and accurate sampling of rhizosphere soil, ensuring that representative soil samples are collected. In addition, the cooperation of the liquid supply and suction system can effectively collect rhizosphere soil samples, and the design of the anti-backflow valve can prevent liquid backflow, ensuring the smooth progress of the sampling process. In summary, the rhizosphere soil sampling device of the application has significant advantages in protecting root systems, improving sampling accuracy and efficiency, and is suitable for soil sampling work in the fields of agricultural science, plant ecology, and environmental monitoring.
[0013] On the basis of the above technical solution, the application can also be improved as follows:
[0014] Further, the lens of the camera module and the LED lamp group are capped by wear-resistant transparent material.
[0015] Preferably, the wear-resistant transparent material is glass.
[0016] Compared with the prior art, the beneficial effects of adopting the above further technical solution are:
[0017] Capping the lens of the camera module and the LED lamp group with wear-resistant transparent material (such as glass) can effectively protect the lens and lamp group from soil abrasion, while maintaining good optical performance, ensuring image clarity and light compensation effect.
[0018] On the basis of the above technical solution, the application can also be improved as follows:
[0019] Further, the camera module has a size of no more than 3.0mmx3.0mmx2.5mm, and a resolution of no less than 400x400 pixels.
[0020] Compared with the prior art, the beneficial effects of adopting the above further technical solution are:
[0021] The size of the camera module is controlled to be not more than 3.0 mm*3.0 mm*2.5 mm and the resolution is not less than 400*400 pixels, so that the end of the sampling device can more flexibly shuttle and operate in the narrow rhizosphere space, and the adaptability to the rhizosphere soil and the sampling flexibility are effectively improved; meanwhile, the high-resolution camera module ensures the definition and details of the returned image, provides a powerful guarantee for accurately judging the position of the root system and controlling the sampling process, and thus the accuracy and success rate of the rhizosphere soil sampling are significantly improved.
[0022] Based on the above technical solutions, the application can be further improved as follows:
[0023] Further, a damping member with an elastic modulus of 0.5-2 MPa is arranged between each joint of the snake bone pipe, and the deformation recovery rate of the damping member is greater than or equal to 95% when the bending angle range of the joint is less than or equal to 60 degrees.
[0024] Compared with the prior art, the beneficial effects of the above further technical solutions are as follows:
[0025] By arranging the damping member with an elastic modulus of 0.5-2 MPa between each joint of the snake bone pipe and ensuring that the deformation recovery rate of the damping member is greater than or equal to 95% when the bending angle range of the joint is less than or equal to 60 degrees, the snake bone pipe has good flexibility and stability during bending and moving, effectively improving its control precision and adaptability in complex soil environments, preventing equipment damage caused by excessive bending, and prolonging the service life.
[0026] Based on the above technical solutions, the application can be further improved as follows:
[0027] Further, the liquid supply pipe and the liquid suction pipe are respectively connected to a liquid supply pump and a liquid suction pump, the flow adjustment range of the liquid supply pump is 0.1-5 mL / s, the suction negative pressure of the liquid suction pump is-10--50 kPa, and both of them are provided with an anti-backflow valve.
[0028] Compared with the prior art, the beneficial effects of the above further technical solutions are as follows:
[0029] The flow and negative pressure adjustment range of the liquid supply pump and the liquid suction pump, together with the anti-backflow valve, can accurately control the soil moistening and sample collection rate, prevent sample pollution, and improve the sampling efficiency and accuracy.
[0030] Based on the above technical solutions, the application can be further improved as follows:
[0031] Further, the switch button assembly includes a power switch, an illumination switch, a photographing button, a video shooting button, an image enlargement button, an image reduction button and a sampling button.
[0032] Compared with the prior art, the beneficial effects of adopting the further technical scheme are:
[0033] By integrating multiple function buttons, the device realizes convenient and accurate control of the sampling process, meets diversified operation requirements, and significantly improves user experience and sampling efficiency.
[0034] On the basis of the above technical scheme, the application can also be improved as follows:
[0035] Further, the sampling button is used to trigger the start and stop of the liquid supply pump and the liquid suction pump, and the liquid suction pump is started later than the liquid supply pump, and the delay time is set to 0.1-1s.
[0036] Compared with the prior art, the beneficial effects of adopting the further technical scheme are:
[0037] This design can accurately control the sampling process, ensure the accuracy and timing coordination of rhizosphere soil moistening and sample extraction, reduce the potential damage risk to the root system, and improve the sampling success rate and operation convenience.
[0038] On the basis of the above technical scheme, the application can also be improved as follows:
[0039] Further, the liquid outlet hole jet direction intersects with the end head advancing direction; the liquid suction hole is an inclined conical hole, the axis of which forms an angle of 30-60° with the second panel, and the hole edge is mirror polished.
[0040] Compared with the prior art, the beneficial effects of adopting the further technical scheme are:
[0041] The jet direction of the liquid outlet hole intersects with the end head advancing direction, which can effectively moisten the soil around the target root system, and the design of the inclined conical liquid suction hole helps to accurately suck rhizosphere soil solution, and the mirror polished hole edge can reduce soil adhesion, both of which can improve sampling accuracy and efficiency while reducing disturbance to the soil and root system.
[0042] On the basis of the above technical scheme, the application can also be improved as follows:
[0043] Further, the photoelectric wire group includes a power line, a signal transmission line and a control line.
[0044] Compared with the prior art, the beneficial effects of adopting the further technical scheme are:
[0045] The integrated design of the photoelectric wire group ensures stable operation of the sampling device in complex soil environments, realizes efficient cooperation of power supply, image signal transmission and operation control, and greatly improves the accuracy and operation convenience of the sampling process.
[0046] The application also provides a rhizosphere soil sampling method based on the device, comprising the following steps:
[0047] (a) According to the distribution range of the target plant root system, the operation direction lever controls the slow insertion of the snake bone pipe into the soil;
[0048] (b) During the insertion process, the image returned by the camera module is observed through the display screen, and the LED lamp group is turned on for light compensation; the bending angle of the snake bone pipe is adjusted according to the position of the root system, so that the end head approaches the target root system;
[0049] (c) When the end head reaches the root system, ensure that the liquid outlet hole is directed towards the root direction, press the sampling button to start the liquid supply pump, and deliver the solution through the liquid outlet hole to wet the soil; the wetting time is adjusted according to the soil dryness degree to wet the soil without water accumulation; the liquid suction pump is started after a delay of 0.1-1s, and the rhizosphere soil solution is extracted through the liquid suction hole;
[0050] (d) Control the snake bone pipe along the growth direction of the root, and monitor the negative pressure fluctuation in real time during the suction process; if the fluctuation exceeds ±5kPa, the process is automatically stopped and an alarm is given;
[0051] (e) Adjust the suction force according to the image on the display screen to ensure stable solution flow and no damage to the root system;
[0052] (f) After the sampling is completed, the snake bone pipe is withdrawn.
[0053] Compared with the prior art, the application has the following beneficial effects:
[0054] The present application controls the slow insertion of the snake bone pipe into the soil through the operation of the direction operating lever, and uses the display screen to observe the images returned by the camera module, turns on the LED light set to supplement light, and can accurately adjust the bending angle of the snake bone pipe according to the root position, so that the end head approaches the target root system. This visual operation and precise control capability greatly improves the sampling accuracy and avoids interference and damage to non-target roots. Secondly, when the end head reaches the root system, the directions and shapes of the liquid outlet hole and the liquid suction hole are reasonably set, and the supply pump and the suction pump are used to work cooperatively, first delivering the solution to wet the soil, and then starting the suction pump to extract the rhizosphere soil solution after a delay, effectively ensuring the stable flow and accurate collection of the soil solution, while avoiding the difficulties and sample pollution caused by dry or excessively wet soil. Furthermore, the negative pressure fluctuation is monitored in real time during the suction process, and if it exceeds the set threshold, the system will automatically stop and alarm, and the suction force is adjusted according to the display screen image to ensure stable solution flow and no damage to the roots. These intelligent monitoring and feedback mechanisms further improve the reliability of the sampling and the safety of the roots. Finally, the snake bone pipe is withdrawn after sampling, and the whole process is efficient and orderly, not only can it obtain representative and accurate rhizosphere soil samples, but also can minimize the damage to the roots and the surrounding soil environment, providing strong support for subsequent soil research and ecological analysis, especially suitable for soil sampling work in the fields of agricultural science, plant ecology and environmental monitoring. BRIEF DESCRIPTION OF DRAWINGS
[0055] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.
[0056] Figure 1 is a front view structural schematic diagram of a preferred embodiment of the rhizosphere soil sampling device of the present application.
[0057] Figure 2 is a perspective structural schematic diagram of Figure 1 .
[0058] Figure 3 is a local structure schematic diagram of the snake bone pipe and the end head in a preferred embodiment of the rhizosphere soil sampling device of the present application.
[0059] Figure 4 is a perspective structural schematic diagram of Figure 3 .
[0060] Figure 5 is another perspective structural schematic diagram of Figure 3 .
[0061] Figure 6 Figure 3 is a schematic view of the partial structure of the snake tube and the end in another preferred embodiment of the rhizosphere soil sampling device of the present application.
[0062] The reference signs in the figure are as follows:
[0063] 100 operating handle,
[0064] 110 display screen,
[0065] 120 switch button assembly,
[0066] 130 direction operating lever,
[0067] 200 sampling tube,
[0068] 210 snake tube,
[0069] 211 damping member,
[0070] 212 guide ring,
[0071] 220 end,
[0072] 201 first panel,
[0073] 202 second panel,
[0074] 221 camera module,
[0075] 222 LED lamp set,
[0076] 223 liquid outlet hole,
[0077] 224 liquid suction hole,
[0078] 230 photoelectric wire set,
[0079] 240 liquid supply pipe,
[0080] 250 liquid suction pipe,
[0081] 260 steel wire rope,
[0082] 270 sleeve. DETAILED DESCRIPTION
[0083] The present application will be described below in conjunction with the accompanying drawings and a specific embodiment.
[0084] In order to make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application.
[0085] It should be noted that similar reference numerals and letters represent similar items in the following drawings, and therefore, once an item is defined in one drawing, it can not be further defined and explained in subsequent drawings.
[0086] Embodiment 1
[0087] Referring to Figures 1 to 6 The rhizosphere soil sampling device described in this embodiment has the following specific structure and composition:
[0088] The operation handle 100 is the operation center of the entire device, and the display screen 110 is integrated thereon, which can clearly display the images and data returned by the camera module 221, facilitating the operator to observe the sampling situation in real time.
[0089] In further embodiments, the display screen 110 can be a touch display screen 110. Such a design makes the operation interface more intuitive and convenient. The operator can directly perform various operations on the display screen 110, such as touching and clicking to control the photographing and video shooting functions of the camera module 221, or adjusting the size of the image through gesture zooming to more clearly view the root details and soil conditions. In addition, the touch display screen 110 can also integrate virtual buttons to replace some physical buttons, further simplifying the structure of the operation handle 100, improving the overall aesthetics and waterproof performance of the device, and providing the operator with a more flexible and efficient operation experience.
[0090] The switch button assembly 120 includes a power switch, a lighting switch, a photographing button, a video shooting button, an image enlargement button, an image reduction button and a sampling button, through which the operator can conveniently control various functions of the device. The direction operating rod 130 is used to control the bending direction of the snake bone pipe 210, thereby adjusting the position and posture of the sampling pipe 200 in the soil.
[0091] The power switch is used to control the power on / off of the entire device.
[0092] The lighting switch controls the on-off of the LED light group 222 on both sides of the camera module 221. In a dimly lit soil environment, by turning on the lighting switch, sufficient light is provided for the camera module 221 to ensure that the captured image is clear and visible, making it easier for the operator to accurately determine the root position and surrounding soil conditions.
[0093] The photograph button is used to capture a static image during the sampling process. When it is necessary to record key information such as the root morphology, soil conditions, or sampling location at a specific moment, pressing this button will take a high-definition photo, providing intuitive image data for subsequent research and analysis.
[0094] The video button is similar to the photograph button, but the difference is that it can record dynamic video. During the operation process, if it is necessary to observe the dynamic changes of the roots, the flow of the solution, or to record the entire sampling process, the video button can be pressed to start the video recording function, providing more dynamic information about the rhizosphere soil environment.
[0095] The image zoom-in button can zoom in the image displayed on the display screen 110, making it easier for the operator to observe the fine structure of the roots, the distribution of soil particles, and the relative position of the end 220 to the roots, and thus more accurately control the movement of the serpentine tube 210 and the sampling operation, improving the sampling accuracy.
[0096] The image zoom-out button is the opposite of the image zoom-in button, and is used to zoom out the image on the display screen 110. When it is necessary to view the overall situation of the entire sampling area, or to return to the normal field of view after zooming in for observation to adjust the macro position of the serpentine tube 210, pressing this button can quickly zoom out the image to help the operator have a comprehensive understanding of the sampling environment.
[0097] The sampling button, as the key trigger button for sampling operation, when pressed, will start the liquid supply pump (not shown in the figure) and the liquid suction pump (not shown in the figure) in sequence, realizing the automatic process of delivering humidifying solution to the rhizosphere soil and extracting samples, effectively simplifying the operation steps, improving the sampling efficiency, and ensuring the accuracy and stability of the sampling process.
[0098] Referring to Figures 3~5 , the sampling tube 200 includes the serpentine tube 210 at the front end of the pipeline and the end 220, which is the key component for soil sampling.
[0099] The snake bone pipe 210 is composed of multiple joints that are sequentially articulated, a guide ring 212 for limiting the steel wire rope 260 is arranged in the snake bone pipe 210, and a damping member 211 is arranged between each joint, the elastic modulus of the damping member 211 is 0.5-2 MPa, and the deformation recovery rate of the damping member 211 is greater than or equal to 95% when the bending angle range of the joint is less than or equal to 60°, such a design makes the snake bone pipe 210 have good flexibility and stability, can flexibly bend and move in a complex soil environment, and can maintain a stable posture during operation and will not be damaged due to excessive bending. The sampling pipe 200 is integrated with a photoelectric wire group 230, a liquid supply pipe 240, a liquid suction pipe 250 and two or four steel wire ropes 260, the photoelectric wire group 230 includes a power line, a signal transmission line and a control line, an outer layer of which is wrapped with a corrosion-resistant insulating material, can provide power and signal transmission for components such as the camera module 221, and has high strength and corrosion resistance, ensuring reliable operation of the device in a soil environment. The three-dimensional space motion control refers to an endoscope, and an operator can accurately control the bending direction of the snake bone pipe 210 through the direction operating lever 130, so that the sampling pipe 200 can smoothly reach the target root system position, and can flexibly shuttle even in a narrow or complex soil space, providing a strong guarantee for rhizosphere soil sampling.
[0100] The end head 220 is spherical at the front end, facilitating movement in soil and avoiding damage to plant roots. An L-shaped notch is formed on the side surface to form the first panel 201 and the second panel 202. The first panel 201 and the end head 220 have an included angle of 170°-180° in the axial direction, Figure 3The first panel 201 is shown with an angle of 180° with the axis direction of the end head 220. The camera module 221 is integrated on the first panel 201, and the size of the camera module 221 is not greater than 3.0mmx3.0mmx2.5mm, and the resolution is ≥400x400 pixels, which can clearly shoot the internal situation of the soil and provide accurate visual information for the operator. For example, OVM9284, OVM6946, IMX225, IMX178, AR0134CS, AR1335, VD6281, CMV2000, GalaxyCore (GalaxyCore) GC2145, etc. Miniature camera sensor module can be selected for use. The LED lamp group 222 is symmetrically arranged on both sides of the camera module 221, which is used to supplement light for the camera module 221 in a relatively dark soil environment, and ensures the clarity of the image. The liquid outlet hole 223 is provided on the front side, and the ejection direction of the liquid outlet hole 223 intersects with the advancing direction of the end head 220, which can uniformly spray the solution into the soil around the target root system. The liquid outlet hole 223 is connected with the liquid supply pipe 240, and the liquid supply pump can deliver the solution to the liquid outlet hole 223 through the liquid supply pipe 240 to wet the soil. The second panel 202 is perpendicular to the axis direction of the end head 220, and the liquid suction hole 224 is arranged thereon. The liquid suction hole 224 is an inclined conical hole, and the axis thereof forms an angle of 30°-60° with the second panel 202. The edge of the hole is mirror polished, which can effectively extract the rhizosphere soil solution and reduce the adhesion of the soil to the hole, thereby improving the sampling efficiency. The liquid suction hole 224 is connected with the liquid suction pipe 250, and the liquid suction pump can extract the rhizosphere soil solution to the sample container (not shown in the figure) through the liquid suction pipe 250.
[0101] In further embodiments, the lens of the camera module 221 and the LED lamp group 222 are capped with a wear-resistant transparent material, preferably glass or organic glass. Glass has good transparency and wear resistance, which can effectively protect the lens of the camera module 221 and the LED lamp group 222 from being worn and scratched by soil particles, and ensure that the lens and the lamp group work stably in complex soil environment for a long time. At the same time, the high transparency of the glass material ensures that the camera module 221 can clearly capture the internal image of the soil, and will not affect the image quality due to wear or pollution of the material. The LED lamp group 222 can also maintain stable brightness output under the protection of the glass, providing reliable light supplement for the camera module 221, and ensuring that clear images can be obtained in a relatively dark soil environment. This design not only prolongs the service life of the camera module 221, but also enhances its reliability and stability in the soil sampling process, providing a strong guarantee for accurate rhizosphere soil sampling.
[0102] Liquid supply and suction system: the liquid supply pipe 240 is connected to a liquid supply pump (not shown in the figure), and the liquid suction pipe 250 is connected to a liquid suction pump (not shown in the figure). The flow rate of the liquid supply pump is adjustable in the range of 0.1-5 mL / s, the suction negative pressure of the liquid suction pump is-10--50 kPa, and both of them are provided with anti-backflow valves, which can effectively prevent liquid backflow and ensure the smooth progress of liquid supply and suction. When sampling, the solution (preferably sterile distilled water) is first delivered to the liquid outlet hole 223 by the liquid supply pump to wet the soil, and then the liquid suction pump is started after a delay of 0.1-1 s. The extension time can be routinely set according to the soil humidity. The rhizosphere soil solution is extracted through the liquid suction hole 224. This design can ensure the stable flow of soil solution and avoid sample contamination or sampling failure due to improper timing of liquid supply and suction.
[0103] Referring to Figure 6 The sampling tube 200 is also provided with a sleeve 270, which mainly serves to provide more comprehensive protection for the sampling tube.
[0104] The sampling method described in this embodiment is as follows: the operator controls the slow insertion of the snake tube 210 into the soil by operating the direction operating rod 130. During the insertion process, the image transmitted back by the camera module 221 is observed through the display screen 110, and the LED light group 222 is turned on for light compensation. According to the position of the root system, the bending angle of the snake tube 210 is adjusted so that the end head 220 approaches the target root system. When the end head 220 reaches the root system, the liquid outlet hole 223 is ensured to face the direction of the root, and the sampling button is pressed to start the liquid supply pump. The solution is delivered through the liquid outlet hole 223 to wet the soil. The wetting time is adjusted according to the degree of soil dryness to be wet without water accumulation. The liquid suction pump is started after a delay of 0.1-1 s, and the rhizosphere soil solution is extracted through the liquid suction hole 224. The snake tube 210 is controlled along the growth direction of the root. During the suction process, the negative pressure fluctuation is monitored in real time, and if it exceeds ±5 kPa, the process is automatically stopped and an alarm is given. The suction force is adjusted according to the image on the display screen 110 to ensure stable solution flow and no damage to the root system. After sampling is completed, the snake tube 210 is withdrawn. Through the rhizosphere soil sampling device provided in this embodiment, accurate and non-destructive sampling of rhizosphere soil can be achieved, which provides strong support for research in the fields of agricultural science, plant ecology and environmental monitoring.
[0105] After completing a sampling, the sampling tube 200 needs to be replaced, or the sampling tube 200, especially the liquid suction pipe 250, needs to be thoroughly cleaned with sterile water to avoid cross contamination of microorganisms.
[0106] In the description of the present application, it needs to be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.
[0107] In addition, the terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise explicitly specified and limited.
[0108] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "fixing" and the like should be understood broadly, for example, it can be fixed connection, or detachable connection, or integral; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through intermediate medium, it can be the internal communication of two elements or the interaction relationship of two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.
[0109] In the present application, unless otherwise explicitly specified and limited, the first feature "above" or "below" the second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, the first feature "above", "above" and "above" the second feature includes that the first feature is directly above and obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature includes that the first feature is directly below and obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.
[0110] The above is only the preferred embodiment of the present application, it should be pointed out that the above preferred embodiment should not be regarded as a limitation of the present application, the protection scope of the present application should be limited by the scope defined by the claims. For those skilled in the art, without departing from the spirit and scope of the present application, a number of improvements and refinements can also be made, which should also be regarded as the protection scope of the present application.
Claims
1. A rhizosphere soil sampling device, characterized in that: include: An operating handle is provided with a display screen, a switch button assembly, and a directional operating lever; the switch button assembly includes: a power switch, a lighting switch, a photo button, a video camera button, an image zoom button, an image zoom button, and a sampling button; the sampling button is used to trigger the start and stop of the liquid supply pump and the liquid suction pump. The liquid suction pump starts with a delay compared to the liquid supply pump, and the delay time is set to 0.1 to 1 second; A sampling tube, comprising a coiled tube and an end cap, wherein an optical fiber assembly, a liquid supply tube, a liquid suction tube, and multiple steel cables are integrated in the sampling tube; the liquid supply tube and the liquid suction tube are connected to a liquid supply pump and a liquid suction pump, respectively; the flow rate adjustment range of the liquid supply pump is 0.1 to 5 mL / s, the suction negative pressure of the liquid suction pump is -50 to -10 kPa, and both are equipped with an anti-backflow valve; the front end of the end cap is a spherical surface, and an L-shaped notch is provided on its surface, wherein the L-shaped notch has a first panel and a second panel, wherein: The angle between the first panel and the axis of the end head is 170° to 180°, and the camera module is integrated thereon. The LED light groups are symmetrically arranged on both sides of the camera module, and a liquid outlet is arranged on the front side, which is connected to the liquid supply pipe. The lens surface of the camera module is embedded in the first panel to avoid direct contact or squeezing with the soil. The second panel is perpendicular to the axis of the end head and is provided with a liquid suction hole connected to the liquid suction tube; the injection direction of the liquid outlet intersects with the propulsion direction of the end head; the liquid suction hole is an inclined conical hole, the axis of which forms an angle of 30° to 60° with the second panel, and the edge of the hole is mirror polished; The snake tube comprises a plurality of sequentially hinged joints, and the bending of the snake tube is achieved by controlling the retraction and extension of the steel wire rope through a direction operating lever.
2. The rhizosphere soil sampling device according to claim 1, characterized in that: The lens and LED light group of the camera module are covered with wear-resistant transparent material.
3. The rhizosphere soil sampling device according to claim 1 or 2, characterized in that: The size of the camera module is no larger than 3.0mm×3.0mm×2.5mm, and its resolution is ≥400×400 pixels.
4. The rhizosphere soil sampling device according to claim 1, characterized in that: A damping member is provided between each joint of the snake tube. The elastic modulus of the damping member is 0.5-2 MPa, and when the joint bending angle is ≤60°, the deformation recovery rate of the damping member is ≥95%.
5. The rhizosphere soil sampling device according to claim 1, characterized in that: The optical wire assembly includes a power line, a signal transmission line and a control line.
6. A rhizosphere soil sampling method, based on the device according to any one of claims 1 to 5, characterized in that: The following steps are involved: (a) According to the root distribution range of the target plant, operate the direction joystick to control the snake tube to slowly insert into the soil; (b) During the insertion process, the image transmitted by the camera module is observed on the display screen, and the LED light group is turned on for fill light. The bending angle of the snake tube is adjusted according to the root position so that the tip is close to the target root system; (c) When the tip reaches the root system, ensure that the outlet is facing the root. Press the sampling button to start the liquid supply pump, and deliver the solution through the outlet to moisten the soil. The wetting time is adjusted according to the dryness of the soil until it is moist without water accumulation. The suction pump starts after a delay of 0.1 to 1 second and extracts the rhizosphere soil solution through the suction hole. (d) The snake tube is controlled along the root growth direction for sampling. The negative pressure fluctuation is monitored in real time during the suction process. If it exceeds ±5kPa, the system automatically stops and issues an alarm. (e) Adjust the suction force according to the image on the display screen to ensure that the solution flows steadily and does not damage the root system; (f) After sampling is completed, the snake tube is withdrawn.
Citation Information
Patent Citations
Portable automatic sampling device for soil solution
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Plant rhizosphere box, rhizosphere box soil sample collecting device and method
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