Self-heating device applied to detection pretreatment of clenbuterol and implementation method of self-heating device

Abstract

The invention discloses a self-heating device applied to pretreatment of clenbuterol detection and an implementation method thereof, and the self-heating device comprises a bottom groove and an upper groove and is made of high-temperature-resistant bakelite. The bottom groove is used for containing a self-heating bag and water and used for heating, the upper groove is used for containing a centrifugal tube containing an animal tissue sample, and the bottom groove and the upper groove are connected through a clamping groove. The outer diameter of the bottom groove is 100 * 35 * 100mm, the inner diameter of the bottom groove is 90 * 30 * 90mm, the outer diameter of the upper groove is 110 * 5 * 110mm, the inner diameter of the upper groove is 90 * 30 * 90mm, nine round test tube holes and a small exhaust hole are formed in the top of the upper groove, the diameter of the round hole of the upper groove is 12mm, the diameter of the exhaust hole is 2mm, a clamping groove is formed in the joint of the lower part of the upper groove and the bottom groove, the width of the clamping groove is 6mm, and the depth of the clamping groove is 5mm. According to the invention, the problem that the sample needs to be heated and extracted when the colloidal gold immunochromatography technology is used for detecting clenbuterol in animal tissues in a non-experimental environment is well solved, and the simplicity and the timeliness of operation are greatly improved.

Description

Technical Field

[0001] The present invention relates to the field of banned veterinary drug detection in the field of food safety detection, and in particular to a method for heating and extracting samples in the process of detecting clenbuterol drugs using a rapid detection method, colloidal gold immunochromatography. Background Art

[0002] Beta-agonists used in lean meat (such as clenbuterol and ractopamine) are illegally added to animal feed to increase lean meat content. However, their residues can cause poisoning in humans, leading to symptoms such as palpitations, headaches, muscle tremors, and even cardiovascular disease. my country explicitly prohibits the use of such drugs in livestock farming, but their illegal use still occurs.

[0003] Regulatory and law enforcement agencies need to conduct random inspections at farms, slaughterhouses, markets, and other locations, but these locations lack professional equipment and personnel, and urgently need a portable, rapid, and instrument-free detection method. Colloidal gold immunochromatography has become the preferred technology for on-site screening of clenbuterol due to its ease of operation.

[0004] However, when using colloidal gold immunochromatography to detect clenbuterol in animal muscle tissues such as pork and beef, the muscle tissue samples need to be pre-treated to extract the clenbuterol in the samples. The most commonly used method is the double boiling method, which heats the centrifuge tube until there is clearly visible tissue fluid penetration, and then the juice is absorbed and added to the colloidal gold test card for testing.

[0005] The traditional method of boiling in water relies on a laboratory environment and requires power and heating equipment. It is not suitable for operation in complex environments, such as the wild or market environments. In order to further reduce the operational difficulty of grassroots law enforcement personnel and improve the timeliness of sample testing, there is an urgent need to invent a portable rapid detection pre-processing device. Summary of the Invention

[0006] The purpose of the present invention is to address the defects and shortcomings of the above-mentioned prior art and propose a self-heating device for pre-treatment of clenbuterol detection and its implementation method, which is a rapid pre-treatment self-heating device that can be widely used in various environments. The device uses high-temperature resistant material Bakelite and is used in a self-heating system to replace the traditional heating method. In terms of structural design, the bottom groove is adapted to the current commercial self-heating bag, and the upper groove design is adapted to the size of the current centrifuge tube, and an exhaust hole is left, ultimately realizing a rapid pre-treatment product that is quickly heated and easy to carry.

[0007] The technical solution adopted by the present invention to solve its technical problems is: a self-heating device for pre-treatment of clenbuterol detection and its implementation method. The device effectively solves the problem of needing to heat and extract samples when using colloidal gold immunochromatography technology to detect clenbuterol in animal tissues in a non-experimental environment, realizes truly rapid detection, and greatly improves the simplicity and timeliness of operation.

[0008] The technical solution adopted by the present invention to solve its technical problems is: a self-heating device for pre-treatment of clenbuterol detection, the device comprising a bottom trough and an upper trough, the outer diameter of the bottom trough is 100*35*100mm, the inner diameter of the bottom trough is 90*30*90mm, the outer diameter of the upper trough is 110*5*110mm, the inner diameter of the upper trough is 90*30*90mm, there are 9 circular test tube holes and a small exhaust hole on the top of the upper trough, the diameter of the circular hole in the upper trough is 12mm, the diameter of the exhaust hole is 2mm, and a card slot is provided at the connection between the lower part of the upper trough and the bottom trough, the width of the card slot is 6mm, and the depth is 5mm.

[0009] The present invention also provides a method for implementing a self-heating device for pre-treatment of clenbuterol detection, the method comprising the following steps:

[0010] Step 1: Add the self-heating bag to the bottom tank.

[0011] Step 2: Add 150 mL of water.

[0012] Step 3: Cover the upper tank cover and place the centrifuge tube containing the animal tissue sample into the centrifuge tube hole in the upper tank.

[0013] Step 4: Heat for 8-10 minutes until tissue fluid seeps out.

[0014] Step 5: Use a pipette to aspirate the tissue fluid and drop it into the colloidal gold test card.

[0015] Beneficial effects:

[0016] 1. The present invention can well realize the rapid detection of clenbuterol in a non-experimental environment.

[0017] 2. The present invention improves the portability and timeliness of detection.

[0018] 3. The present invention can effectively assist law enforcement agencies in supervising and combating such food safety issues.

[0019] 4. The device of the present invention uses bakelite, a high-temperature resistant material, which can effectively insulate heat. The device is portable and easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a physical diagram of the device of the present invention.

[0021] Figure 2Schematic diagram of the design of the device of the present invention.

[0022] Figure 3 Flow chart of the method of the present invention. DETAILED DESCRIPTION

[0023] The present invention will be described in further detail below with reference to the accompanying drawings.

[0024] In the description of the present invention, it should be noted that terms such as "upper," "bottom," and "top" indicating orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings. These terms are used solely to facilitate the description of the present invention and to simplify the description. They are not intended to indicate or imply that the device or component referred to must have a specific orientation, be constructed, or operate in a specific manner. Therefore, they should not be construed as limitations on the present invention.

[0025] like Figure 1 and Figure 2 As shown, the present invention provides a self-heating device for pre-treatment of clenbuterol detection, which includes a bottom trough and an upper trough. The outer diameter of the bottom trough is 100*35*100mm, and the inner diameter of the bottom trough is 90*30*90mm. The outer diameter of the upper trough is 110*5*110mm, and the inner diameter of the upper trough is 90*30*90mm. There are 9 circular test tube holes and a small exhaust hole on the top of the upper trough. The diameter of the circular hole in the upper trough is 12mm, and the diameter of the exhaust hole is 2mm. A card slot is provided at the connection between the lower part of the upper trough and the bottom trough. The width of the card slot is 6mm and the depth is 5mm.

[0026] The bottom tank of the device of the present invention is used to hold the self-heating bag and water for heating, and the upper tank is used to place the centrifuge tube containing the animal tissue sample. The bottom tank and the upper tank are connected by a clamping groove, which can effectively fix and prevent sliding.

[0027] The device of the present invention uses bakelite, a high-temperature resistant material, which can effectively insulate heat. The device of the present invention is portable and easy to operate.

[0028] like Figure 3 As shown, the present invention provides a method for implementing a self-heating device for pre-treatment of clenbuterol detection, the method comprising the following steps:

[0029] Step 1: Add the self-heating bag to the bottom tank

[0030] Step 2: Add 150mL of water.

[0031] Step 3: Cover the upper tank cover and place the centrifuge tube containing the animal tissue sample into the centrifuge tube hole in the upper tank.

[0032] Step 4: Heat for 8-10 minutes until tissue fluid seeps out.

[0033] Step 5: Use a pipette to aspirate the tissue fluid and drop it into the colloidal gold test card.

[0034] For those skilled in the art, the device of the present invention is not limited to the exemplary implementation described above. Any technician familiar with this technical field can replace or change the technical solution and concept of the present invention within the technical scope disclosed by the present invention, and the replacement or change should be covered by the protection scope of the technology of the present invention.

Claims

1. A self-heating device for pre-treatment of clenbuterol detection, characterized by: The device comprises a bottom trough and an upper trough, the top of the upper trough is provided with 9 circular test tube holes and a small exhaust hole, and the bottom trough and the upper trough are connected by a card slot; The outer diameter of the bottom trough is 100*35*100mm, the inner diameter of the bottom trough is 90*30*90mm, the outer diameter of the upper trough is 110*5*110mm, the inner diameter of the upper trough is 90*30*90mm, and a card slot is provided at the connection between the lower part of the upper trough and the bottom trough; The card slot width is 6mm, the depth is 5mm, the diameter of the upper slot hole is 12mm, and the diameter of the exhaust hole is 2mm.

2. The self-heating device for pre-treatment of clenbuterol detection according to claim 1, characterized in that: The bottom trough and the upper trough are made of high temperature resistant material bakelite.

3. A method for implementing a self-heating device for pre-treatment of clenbuterol detection, characterized in that: The method comprises the following steps: Step 1: Add the self-heating bag to the bottom tank; Step 2: Add 150 mL of water; Step 3: Cover the upper tank cover and place the centrifuge tube containing the animal tissue sample into the centrifuge tube hole in the upper tank; Step 4: Heat for 8-10 minutes until tissue fluid exudates; Step 5: Use a pipette to aspirate the tissue fluid and drop it into the colloidal gold test card.

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