Method for determining contents of various components in blumea balsamifera oil

Abstract

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for determining the content of various components in blumea balsamifera oil. According to the method, a gas chromatography-mass spectrometry (GC-MS) technology is adopted, and chromatographic conditions are as follows: a chromatographic column is an SH-Stabilwax (30m * 0.32 mm, and the film thickness is 0.25 [mu] m) capillary column; the temperature of a sample inlet is 230 DEG C; the split ratio is 10: 1; the sample size is 1 microliter; the mass spectrum conditions are as follows: the ion source temperature is 200 DEG C; the interface temperature is 230 DEG C; the ionization mode is electron impact ion (EI); the detection voltage is 0.2 KV; the solvent is delayed for 2 minutes; the mass number scanning range is 30 to 400 amu; and SIM mode. According to the method disclosed by the invention, the contents of alpha-pinene, camphene, beta-pinene, limonene, p-cymene, camphor, alpha-coumarin, linalool, bornyl acetate, beta-caryophyllene, myrtenal, alpha-caryophyllene, L-borneol, perillaldehyde, caryophyllene oxide, guaiacol, beta-eudesmol and xanthoxylin in the blumea balsamifera oil can be simultaneously determined; the method provided by the invention has good precision, repeatability and stability, and the quality consistency of the blumea balsamifera oil can be quickly, accurately and effectively evaluated through detection of multiple components.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for determining the contents of multiple components in Blumea balsamifera oil. Background Art

[0002] Blumea balsamifera (Blumea balsamifera) oil is extracted by pressing and separating the crude sublimates of the leaves of Blumea balsamifera (L.) DC., a plant of the Asteraceae family. It has the effects of clearing heat and detoxifying, reducing swelling and relieving pain, and relieving itching. It is listed in the 2019 edition of the "Quality Standards for Traditional Chinese and Ethnic Medicinal Materials in Guizhou Province" and is one of the key TCM varieties developed in Guizhou Province. As a major ingredient in Guizhou specialty ethnomedicines such as Yanlishuang Drops, Jinhoujian Spray, Wanjinxiang Aerosol, and Yankang Lozenges, this product has demonstrated significant clinical efficacy and generated positive socioeconomic benefits. Blumea balsamifera oil has a complex composition, and existing local standards only measure L-borneol content. This single indicator fails to reflect its overall efficacy and cannot fully control its quality. Although studies have used GC to simultaneously determine the contents of six components in Blumea balsamifera oil, this method has low sensitivity, measures fewer compounds, and fails to identify the quality-differentiating components of Blumea balsamifera oil.

[0003] Gas chromatography-mass spectrometry (GC-MS) combines the efficient separation capabilities of gas chromatography (GC) with the highly sensitive detection and structural identification capabilities of mass spectrometry (MS), enabling the detection of trace components and accurate structural identification. Furthermore, this technique offers advantages such as accurate quantitative analysis, strong anti-interference capabilities, a high degree of automation, and high reliability of results. However, there are no reports in the literature on the use of GC-MS for the determination of multiple content indicators in Ailanthus flavonoids oil.

[0004] Therefore, the current key research direction is to find a method that can simultaneously determine α-pinene, camphene, β-pinene, limonene, p-cymene, camphor, α-guarene, linalool, bornyl acetate, β-caryophyllene, myrtenal, α-caryophyllene, L-borneol, perillaldehyde, caryophyllene oxide, guaiacol, β-cineole, and zanthoxylum bungeol, which can be used to effectively evaluate the quality consistency of Artemisia selengensis oil. Summary of the Invention

[0005] In order to solve the above technical problems existing in the prior art, the present invention provides a method for determining the contents of multiple components in Blumea balsamifera oil, comprising the following steps:

[0006] A method for determining the contents of multiple components in Artemisia selengensis oil is used for simultaneously determining the contents of α-pinene, camphene, β-pinene, limonene, p-cymene, camphor, α-guruene, linalool, bornyl acetate, β-caryophyllene, myrtenal, α-caryophyllene, L-borneol, perillaldehyde, caryophyllene oxide, guaiacol, β-cineole, and zanthoxylum bungeol in Artemisia selengensis oil. The method adopts gas chromatography-mass spectrometry (GC-MS) for determination, and the specific conditions are as follows:

[0007] Chromatographic conditions: Column: SH-Stabilwax (30 m × 0.32 mm, film thickness 0.25 μm) capillary column; injection port temperature 230°C; split ratio 10:1; injection volume 1 μL;

[0008] Mass spectrometry conditions: ion source temperature 200°C; interface temperature 230°C; ionization mode: electron impact ionization (EI); detection voltage: 0.2 kV; solvent delay 2 min; mass number scanning range: 30-400 amu; SIM mode.

[0009] Furthermore, in the chromatographic conditions, the column oven temperature program is as follows:

[0010]

[0011] Furthermore, in the mass spectrometry conditions, the detected ion information of each component is as follows:

[0012]

[0013] Furthermore, naphthalene was used as an internal standard.

[0014] Furthermore, the test solution used is a test solution containing 50 μg / mL of Artemisia selengensis oil and 50 ng / mL of naphthalene.

[0015] Furthermore, the test solution is prepared by the following method: accurately weighing linalool oil, adding ethyl acetate to dissolve it, and configuring the naphthalene internal standard solution into a test solution containing 50 μg / mL of linalool oil and 50 ng / mL of naphthalene.

[0016] Furthermore, the naphthalene internal standard solution is prepared by taking a naphthalene reference substance and preparing a 500 ng / mL internal standard solution with ethyl acetate.

[0017] Furthermore, the reference substance stock solution is prepared by the following method:

[0018] Accurately weigh L-borneol and other reference substances, place them in a 10 mL volumetric flask, add ethyl acetate to dilute to the mark, and prepare L-borneol (1.077 mg / mL), β-caryophyllene (1.4601 mg / mL), camphor (1.002 mg / mL), caryophyllene oxide (1.578 mg / mL), zanthoxylum oil (0.853 mg / mL), linalool (2 mg / mL), α-caryophyllene (10 mg / mL), guaiacol (1.650 mg / mL), β-pinene (9 .902 mg / mL), β-eudesmol (0.862 mg / mL), (+)-limonene (4.556 mg / mL), bornyl acetate (4.3068 mg / mL), α-pinene (5 mg / mL), camphene (1.074 mg / mL), perillaldehyde (2 mg / mL), α-guineenol (10 mg / mL), myrtenal (1.516 mg / mL), and p-cymene (4.792 mg / mL) reference substance stock solutions were stored at -20°C for later use. The reference substance stock solutions were used to dilute reference substance solutions of different concentrations and draw a standard curve.

[0019] Compared with the prior art, the technical effects created by the present invention are embodied in:

[0020] The present invention adopts gas chromatography-mass spectrometry (GC-MS) technology, and the chromatographic conditions are as follows: chromatographic column: SH-Stabilwax (30m×0.32mm, film thickness 0.25μm) capillary column; injection port temperature 230°C; split ratio 10:1; injection volume 1μL; mass spectrometry conditions: ion source temperature 200°C; interface temperature 230°C; ionization mode is electron impact ionization (EI); detection voltage: 0.2KV; solvent delay 2min; mass number scanning range: 30-400amu; SIM mode. The method disclosed by the present invention can simultaneously determine the contents of L-borneol, β-caryophyllene, camphor, caryophyllene oxide, zanthoxylum, linalool, α-caryophyllene, guaiacol, β-pinene, β-cineole, limonene, bornyl acetate, α-pinene, camphene, perillaldehyde, α-gulene, myrtenal, and p-cymene in the Artemisia annua balsamifera oil. The method disclosed by the present invention has good precision, repeatability, and stability, and can effectively evaluate the quality consistency of the Artemisia annua balsamifera oil. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1The GC-MS SIM chromatograms of the reference solution (A), the test solution (B), and the blank solution (C) are shown. Note: 1: α-pinene, 2: camphene, 3: β-pinene, 4: limonene, 5: p-cymene, 6: camphor, 7: α-guarene, 8: linalool, 9: bornyl acetate, 10: β-caryophyllene, 11: myrtenal, 12: α-caryophyllene, 13: L-borneol, IS: naphthalene, 14: perillaldehyde, 15: caryophyllene oxide, 16: guaiacol, 17: β-cineole, 18: xanthol.

[0022] Figure 2 This is a cluster analysis diagram of 10 batches of Artemisia annua sesame oil samples.

[0023] Figure 3 This is the PCA scatter score plot of 10 batches of Ai Na sesame oil samples.

[0024] Figure 4 is a 200-response ranking test of the OPLS-DA model.

[0025] Figure 5 This is the OPLS-DA score graph of 10 batches of Ai Na sesame oil samples.

[0026] Figure 6 This is the VIP value diagram of each component of 10 batches of Ai Na sesame oil samples using OPLS-DA. DETAILED DESCRIPTION

[0027] The technical solution of the present invention is further defined below in conjunction with specific implementation methods, but the scope of protection required is not limited to the description.

[0028] Example:

[0029] 1 Materials and Methods

[0030] 1.1 Materials

[0031] 1.1.1 Instruments

[0032] Gas chromatography-mass spectrometry (Typ. GC-MS-TQ8050NX, Shimadzu Enterprise Management (China) Co., Ltd.), electronic balance (Typ. EL204, METTLER TOLEDO Shanghai Co., Ltd.)

[0033] 1.1.2 Drug testing

[0034] L-Borneol (Lot.NO WP23110201), β-Caryophyllene (Lot.NO WP23110105), Zanthoxylum bungeol (Lot.NO WP24061302), Linalool (Lot.NO WP24051103), α-Caryophyllene (Lot.NO WP24061401), β-Pinene (Lot.NO WP24022005), β-Cineole (Lot.NO WP23092503), (+)-Limonene (Lot.NO WP24012311), Bornyl Acetate (Lot.NO AF20071901), α-Pinene (Lot.NO WP24061212), Camphene (Lot.NO WP24042604), Perillaldehyde (Lot.NO WP23102507), α-guinene (Lot. NO WP24061113), and p-cymene (Lot. NO WP24060301) were purchased from Sichuan Weikeqi Biotechnology Co., Ltd.; camphor (Lot. NO 110747-202412) and guaiacol (Lot. NO ZZS-20-H219-A1) were purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.; caryophyllene oxide (Lot. NO AFD10601) and myrtenal (Lot. NO AF21081004) were purchased from Chengdu Aifa Biotechnology Co., Ltd.; and naphthalene (Lot. NO 21050063) was purchased from Tanmo Quality Inspection-Standard Material Center. Ethyl acetate (HPLC grade, Shanghai MacLean Biochemical Technology Co., Ltd.) was used.

[0035] 1.1.3 Sample Information

[0036] The origin information of 10 batches of Artemisia annua sesame oil is shown in Table 1, which was provided by Guizhou Huangguoshu Shushuang Pharmaceutical Co., Ltd.

[0037] Table 1 Information of samples of Ai Na sesame oil

[0038]

[0039]

[0040] 1.2 Methods

[0041] 1.2.1 GC-MS conditions

[0042] Chromatographic conditions: Chromatographic column: SH-Stabilwax (30 m × 0.32 mm, film thickness 0.25 μm) capillary column; injection port temperature 230°C; split ratio 10:1; injection volume 1 μL; temperature program details are shown in Table 2.

[0043] Table 2 Column oven temperature program

[0044]

[0045] Mass spectrometry conditions: ion source temperature 200°C; interface temperature 230°C; electron impact ionization (EI) mode; detection voltage: 0.2 kV; solvent delay 2 min; mass scan range: 30–400 amu; SIM mode. Detected ion information for each component is shown in Table 3.

[0046] Table 3 Ion information of multiple index components detected in flavonoid oil

[0047]

[0048] 1.2.2 Solution preparation

[0049] 1.2.2.1 Reference substance stock solution:

[0050] Accurately weigh L-borneol and other reference substances, place them in a 10 mL volumetric flask, add ethyl acetate to dilute to the mark, and prepare α-pinene (5.000 mg / mL), camphene (1.074 mg / mL), β-pinene (9.902 mg / mL), (+)-limonene (4.556 mg / mL), p-cymene (4.792 mg / mL), camphor (1.002 mg / mL), α-galbanum (10.00 mg / mL), linalool (2.000 mg / mL), bornyl acetate (4. The reference substance stock solutions of 1, 2, 3, 4, 5-dimethicone (307 mg / mL), β-caryophyllene (1.460 mg / mL), myrtle aldehyde (1.516 mg / mL), α-caryophyllene (10 mg / mL), L-borneol (1.077 mg / mL), perillaldehyde (2.000 mg / mL), caryophyllene oxide (1.578 mg / mL), guaiacol (1.650 mg / mL), β-eudesmol (0.8620 mg / mL), and zanthoxylum bungeanum (0.8530 mg / mL) were stored at -20°C for use.

[0051] 1.2.2.2 Preparation of internal standard solution:

[0052] Accurately weigh the naphthalene reference substance and prepare a 500 ng / mL internal standard solution with ethyl acetate. 1.2.2.3 Preparation of test solution

[0053] Accurately weigh 0.1 g of Artemisia selengensis oil and dissolve it in ethyl acetate. The naphthalene internal standard solution was prepared into a test solution containing 50 μg / mL of Artemisia selengensis oil and 50 ng / mL of naphthalene.

[0054] 1.2.3 Methodological Investigation

[0055] 1.2.3.1 Specificity Investigation

[0056] The mixed reference solution was accurately aspirated separately (the stock solution of each reference substance was accurately aspirated and diluted with ethyl acetate to prepare a mixed reference solution containing the following component concentrations: α-pinene 1.88 μg / mL, camphene 0.10 μg / mL, β-pinene 0.62 μg / mL, limonene 0.68 μg / mL, p-cymene 0.04 μg / mL, camphor 9.02 μg / mL, α-guarene 0.10 μg / mL, linalool 0.38 μg / mL, bornyl acetate 0.10 μg / mL, β- -caryophyllene 3.65μg / mL, myrtle aldehyde 0.25μg / mL, α-caryophyllene 0.05μg / mL, L-borneol 16.16μg / mL, perillaldehyde 0.13μg / mL, caryophyllene oxide 0.99μg / mL, guaiacol 0.13μg / mL, β-eudesmol 0.16μg / mL, zanthoxylum 0.85μg / mL), test solution and blank control solution (ethyl acetate) 1μL, according to the GC-MS conditions examined in "1.2.1", the chromatogram is shown. Figure 1 The results showed that there was no significant interference in the determination of 18 components in Ai Na sesame oil. The chromatographic conditions under "1.2.1" were used for sample injection analysis. The chromatogram is shown in Figure 1 In the chromatogram of the test sample, the retention time of each component was consistent with that of the reference sample, and the chromatogram of the blank control solution showed no corresponding peaks at the same retention time. The results showed that there was no significant interference in the determination of the 18 components in Ailanthus flavonoids oil.

[0057] 1.2.3.2 Linear Relationship Investigation

[0058] Accurately pipette an appropriate amount of reference solution to prepare a series of mixed reference solutions, and inject the samples according to the GC-MS conditions under "1.2.1". Use the concentration of each reference solution as the horizontal axis (X) and the peak area as the vertical axis (Y) to draw a standard working curve and calculate the regression equation. The results are shown in Table 3. R 2 All of them were greater than 0.9990, indicating that the 18 components had a good linear relationship within the corresponding concentration range.

[0059] Table 3 Regression curve equations of 18 components including α-pinene

[0060]

[0061] 1.2.3.3 Precision test

[0062] Take the mixed reference solution and inject it for analysis according to the GC-MS conditions under "1.2.1". Inject it 6 times continuously within 1 day, record the peak area, and calculate its intra-day precision. The results are detailed in Table 4. Accurately aspirate the same mixed reference solution and inject it continuously for 3 days. Measure it 3 times a day, record the peak area, and calculate the inter-day precision. The results are detailed in Table 5. The intra-day precision and inter-day precision of the 18 components were all less than 3%, indicating that the instrument precision was good.

[0063] Table 4 Intra-day precision test of 18 components in Ai Na sesame oil

[0064]

[0065] Note: AN1: α-pinene, AN2: camphene, AN3: β-pinene, AN4: limonene, AN5: p-cymene, AN6: camphor, AN7: α-guarene, AN8: linalool, AN9: bornyl acetate, AN10: β-caryophyllene, AN11: myrtenal, AN12: α-caryophyllene, AN13: L-borneol, AN14: perillaldehyde, AN15: caryophyllene oxide, AN16: guaiacol, AN17: β-eudesmol, AN18: zanthoxylum oil

[0066] Table 5 Inter-day precision test of 18 components in Ai Na sesame oil

[0067]

[0068] 1.2.3.4 Stability test

[0069] Precision pipette the sample solution prepared under the treatment conditions described in "1.2.2.3." Measure the peak areas of the 23 components using the GC-MS conditions described in "1.2.1" at 0, 2, 4, 6, 8, 12, and 24 hours. The results are detailed in Table 6. The RSDs for the peak areas of the 18 components ranged from 0.45 to 2.44%, indicating good stability of the sample solution over 24 hours.

[0070] Table 6 Stability test of 18 ingredients in Blumea sesame oil

[0071]

[0072] 1.2.3.5 Repeatability test

[0073] Take 0.1 g of Artemisia selengensis oil, accurately weigh it, and prepare 6 test solutions in parallel according to the method under "1.2.2.3". Sampling and determination are carried out according to the GC-MS conditions under "1.2.1", and the peak areas are recorded and the contents are calculated. The results are detailed in Table 7. The RSDs of the contents of 18 components are all less than 3%, indicating that the method has good repeatability.

[0074] Table 7 Repeatability test of 18 components in flavonoids oil

[0075]

[0076] 1.2.3.6 Sample recovery

[0077] Take 6 portions of Artemisia selengensis oil with known contents of 18 components, accurately weigh 0.05 g of each portion, and accurately add mixed reference solution (about 100% of the target component content) to each portion. Prepare 6 test solution in parallel according to the method under "1.2.2.3". Sampling and determination are carried out according to the GC-MS conditions under "1.2.1", and the peak areas are recorded and the average recovery rate is calculated. The results are shown in Table 8. The average recovery rate of each component is 97.40% to 103.44%, and the RSD is less than 3%, indicating that the method has good accuracy.

[0078] Table 8 Recovery test of 18 components in flavonoids oil

[0079]

[0080]

[0081]

[0082] 1.2.4 Data Processing

[0083] SPSS25.0 and SIMCA 14.1 were used to process and analyze the content results of 18 components in 10 batches of Artemisia annua sesame oil.

[0084] 2 Results

[0085] 2.1 Measurement results

[0086] The contents of 18 index components in 10 batches of Artemisia selengensis oil samples are shown in Table 9.

[0087] Table 9 Determination of 18 components in 10 batches of Artemisia selengensis oil

[0088]

[0089] 2.2 Chemical pattern recognition

[0090] 2.2.1 Cluster Analysis (CA)

[0091] The data of 18 components in 10 batches of samples were used as variables and imported into SPSS22.0 software for cluster analysis. The Ward method was used and Euclidean distance was used as the metric for cluster analysis of 10 batches of Ailanthus oil. Figure 2The results show that when the average Euclidean distance is 19, all samples are divided into three categories: Category I is S1-S2, S5, S7-S9; Category II is S3, S4, S10; Category III is S6.

[0092] 2.2.2 Principal Component Analysis (PCA)

[0093] The contents of 18 components of 10 batches of Ai Na sesame oil samples were imported into SIMCA 11.0 software for principal component analysis, and the scaling method was Ctr (centering). Figure 3 As shown in the figure, the results showed that the 10 batches of Artemisia selengensis oil were mainly divided into three categories: Category I was S1-S2, S5, S7-S9; Category II was S3, S4, S10; and Category III was S6. This result was consistent with the results of cluster analysis.

[0094] 2.2.3 Partial Least Squares Analysis (OPLS-DA)

[0095] In order to better analyze the differences between the 10 batches of Ai Na sesame oil samples and to find markers of quality differences in Ai Na sesame oil, supervised OPLS-DA was used for modeling. The content data of 18 components of the 10 batches of Ai Na sesame oil samples were imported into SIMCA 14.1, and OPLS-DA was performed on these 10 batches of samples. The principal component regression coefficient Q of the OPLS-DA model established in this study was 2 =0.892, model discrimination parameter R 2 Y=0.959, matrix fruiting rate R 2 X = 0.989, and its values are all greater than 0.5, indicating that the model is stable and has good predictive ability. 200 response ranking tests were further used, such as Figure 4 The results show that all R 2 and Q 2 The values are all smaller than the original values, and Q 2 The regression line has a negative intercept with the Y axis, indicating that the model is valid and there is no overfitting. This model can be used to screen the quality difference markers of Ai Na sesame oil. Figure 5 As shown in the figure, the 10 batches of Artemisia selengensis oil samples were divided into three categories: Category I was S1-S2, S5, S7-S9; Category II was S3, S4, S10; and Category III was S6. This result was consistent with the results of cluster analysis and principal component analysis.

[0096] The VIP value in the OPLS-DA model can be used to screen out the components that contribute most to the above sample classification. The VIP value is shown in Figure 6, with VIP value > 1 as the meaningful variable for search, the ingredients ranked in order of VIP value are β-caryophyllene, zanthoxylum, camphor, α-pinene, and L-borneol. These ingredients may be the quality markers that cause differences between different batches of samples.

[0097] 3. Conclusion

[0098] The present invention adopts GC-MS to detect Artemisia annua oil, and establishes a method for simultaneously determining L-borneol, β-caryophyllene, camphor, caryophyllene oxide, zanthoxylum, linalool, α-caryophyllene, guaiacol, β-pinene, β-cineole, (+)-limonene, bornyl acetate, α-pinene, camphene, perillaldehyde, α-gulene, myrtenal and p-cymene. The method has good precision, repeatability and stability, and can effectively evaluate the quality consistency of Artemisia annua oil.

[0099] OPLS-DA screening identified five differentially expressed compounds: β-caryophyllene, zanthoxylum, camphor, α-pinene, and L-borneol. These compounds can be used to characterize the quality differences between herbal medicines from different origins. Content determination of the selected index components provides a comprehensive characterization of the differential properties of mugwort oil. L-borneol, with its high content, is a key component of investigation. Its antibacterial, anti-inflammatory, central nervous system stimulant, and analgesic properties are consistent with the clinical application of mugwort oil. Camphor exhibits anti-inflammatory, analgesic, antibacterial, antitussive, and antipruritic properties, while β-caryophyllene exhibits strong anti-inflammatory, analgesic, and detumescent properties. Zanthoxylum has been shown to inhibit platelet aggregation and can suppress drug-induced uterine and bladder muscle contractions. Its derivatives also possess antibacterial and anti-insecticide activities. α-pinene exhibits anti-inflammatory, anti-tumor, antibacterial, and anti-allergic pharmacological properties.

[0100] In summary, this experiment established a rapid and stable method for the content determination of Artemisia selengensis oil, and used chemical pattern analysis to screen out the quality difference substances β-caryophyllene, zanthoxylum bungeanum, camphor, α-pinene and L-borneol, which can provide a strong basis for the quality control of Artemisia selengensis oil and provide a reference for its quality control evaluation.

[0101] Finally, it should be noted that the above embodiments are merely representative examples of the present invention. Obviously, the technical solutions of the present invention are not limited to the above embodiments and are subject to numerous variations. All variations that can be directly derived or conceived by a person of ordinary skill in the art from the disclosure of the present invention should be considered within the scope of protection of the present invention.

Claims

1. A method for determining the contents of multiple components in mugwort balsamifera oil, for simultaneously determining the contents of α-pinene, camphene, β-pinene, limonene, p-cymene, camphor, α-guruene, linalool, bornyl acetate, β-caryophyllene, myrtenal, α-caryophyllene, L-borneol, perillaldehyde, caryophyllene oxide, guaiacol, β-cineole, and zanthoxylum in mugwort balsamifera oil, characterized in that: Gas chromatography-mass spectrometry (GC-MS) was used for determination, and the specific conditions were as follows: Chromatographic conditions: Column: SH-Stabilwax (30 m × 0.32 mm, film thickness 0.25 μm) capillary column; injection port temperature 230°C; split ratio 10:1; injection volume 1 μL; Mass spectrometry conditions: ion source temperature 200°C; interface temperature 230°C; ionization mode: electron impact ionization (EI); detection voltage: 0.2 kV; solvent delay 2 min; mass number scanning range: 30-400 amu; SIM mode.

2. the method for measuring the content of multiple components in Blumea balsamifera oil according to claim 1, is characterized in that, In the chromatographic conditions, the column oven temperature program is as follows:

3. the method for measuring the content of multiple components in Blumea balsamifera oil according to claim 1, is characterized in that, In the mass spectrometry conditions, the detected ion information of each component is as follows:

4. The method for measuring the contents of various components in Blumea balsamifera oil according to claim 1, wherein Naphthalene was used as the internal standard.

5. the method for measuring the content of multiple components in Blumea balsamifera oil according to claim 1, is characterized in that, The test solution contains 50 μg / mL of Artemisia selengensis oil and 50 ng / mL of naphthalene.

6. The method for measuring the contents of various components in Blumea balsamifera oil according to claim 5, wherein The test solution is prepared by the following method: accurately weighing balsam pear oil, adding ethyl acetate to dissolve it, and configuring a naphthalene internal standard solution into a test solution containing 50 μg / mL of balsam pear oil and 50 ng / mL of naphthalene.

7. The method for measuring the contents of various components in Blumea balsamifera oil according to claim 6, wherein The naphthalene internal standard solution is prepared by taking a naphthalene reference substance and adding ethyl acetate to prepare a 500 ng / mL internal standard solution.

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