Method for exciting enzymatic activity in seeds through electromagnetic field
The seeds are processed by electromagnetic magnetic fields, and the alternating magnetic fields with specific frequency and magnetic induction intensity are used to activate the enzyme activity in the seeds, which solves the problems of gene mutation and chemical residues in traditional methods, and achieves the precise regulation of enzyme activity and the improvement of crop safety.
Patent Information
- Application Number
- CN202510661806.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-22
- Publication Date
- 2025-08-19
AI Technical Summary
Traditional seed enzyme activity treatment methods have problems such as non-targeted gene mutation risk, weakening the independent response ability of seedlings to face adversity, and chemical residues that cause food safety and ecological pollution.
The electromagnetic magnetic field is used to stimulate the internal enzyme activity of seeds. Through alternating magnetic field treatment with specific frequency and magnetic induction intensity, combined with temperature control and infrared monitoring, amylase and antioxidant enzymes are activated in a directional manner to avoid defects in chemical mutagenesis and hormone treatment.
The targeted and precise activation of enzyme activities is achieved, the seed germination speed and seedling stress resistance are improved, the crop safety and ecological friendliness are ensured, non-targeted gene mutations and chemical residues are avoided, and the rapid germination of seeds and the healthy growth of seedlings is promoted.
Abstract
Description
Technical Field
[0001] The present invention relates to the field of electromagnetic field treatment technology, in particular to a method for stimulating enzyme activity inside seeds through an electromagnetic magnetic field. Background Art
[0002] During the critical stage of seed germination and seedling growth, key enzymes are like the core components of the "life engine" and play an irreplaceable role. Amylase provides initial energy for seed germination by catalyzing the hydrolysis of starch into soluble sugars. Its activity directly determines the germination speed and uniformity. Peroxidase is involved in clearing the reactive oxygen produced during the germination process, protecting the stability of the cell membrane structure and avoiding oxidative damage. Superoxide dismutase cooperates with peroxidase to build an antioxidant defense network and enhance the stress resistance of seedlings. If the activity of key enzymes is inhibited, insufficient energy supply will lead to germination stagnation, and the accumulation of oxidative stress will induce programmed cell death, ultimately reducing crop yield and quality. Precise regulation of key enzyme activity is an important breakthrough for efficient agricultural production. During seed germination and seedling growth, the activity of key enzymes directly affects the germination rate, energy metabolism efficiency, and drought resistance, cold resistance, and disease resistance. However, traditional methods for regulating enzyme activity in seeds usually use chemical mutagenesis or hormone treatment. Although the above treatment methods can regulate enzyme activity, chemical mutagenesis may induce non-targeted gene mutations, resulting in uncontrollable plant traits. Exogenous hormone treatment may weaken the seedlings' ability to respond autonomously to adversity, and hormone or mutagen residues may be transmitted through the food chain, causing food safety and ecological pollution problems. Therefore, the present invention uses a method of stimulating internal enzyme activity in seeds through an electromagnetic magnetic field to directionally stimulate the endogenous enzyme system in seeds to solve the technical difficulties in the traditional enzyme regulation process. Summary of the Invention
[0003] In order to overcome the problems that traditional chemical mutagenesis or hormone treatment can regulate seed enzyme activity, but there is a risk of non-targeted gene mutation, weakening the seedling's ability to respond autonomously to adversity, and chemical residues causing food safety and ecological pollution.
[0004] The technical solution of the present invention is: a method for stimulating enzyme activity inside seeds through an electromagnetic magnetic field, and the preparation steps are as follows:
[0005] Step 1: Seed screening, select healthy seeds that are plump, free of disease and insect pests;
[0006] Step 2: Clean and disinfect the seeds. Wash the seeds carefully with deionized water to remove surface impurities. Then soak the seeds in deionized water at 23-25°C for 6-24 hours. Then transfer them to 0.1% sodium hypochlorite solution and soak for 10 minutes. Then rinse them with clean water three times and dry them for later use.
[0007] Step 3: Seed loading: Spread the pre-treated seeds evenly on the tray, ensuring that the thickness of the single layer does not exceed 2 cm. Then place the tray in the center of the treatment chamber of the low-frequency electromagnetic generator.
[0008] Step 4: Frequency selection. According to the need to enhance the activity of the target enzyme, a specific frequency and magnetic induction intensity are selected. If the target enzyme is amylase, a low-frequency magnetic field of 50 Hz and a magnetic induction intensity of 5 mT should be selected. If the target enzyme is an antioxidant enzyme, a medium-frequency magnetic field of 75 Hz and a magnetic induction intensity of 8 mT should be selected.
[0009] Step 5: Magnetic field treatment: start the equipment, apply an alternating magnetic field, and simultaneously start the temperature control system to maintain a constant temperature environment of 25°C. The treatment time lasts for 20-40 minutes.
[0010] Step 6: Dynamic monitoring: Use an infrared thermal imager to monitor changes in seed surface temperature to ensure there is no local overheating;
[0011] Step 7: Static treatment: turn off the magnetic field and place the treated seeds in a dark room for 10 minutes to eliminate residual electromagnetic effects and promote the stability of the enzyme system;
[0012] Step 8: Post-treatment: The seeds that have been statically treated can be sown immediately or stored in the dark at 25°C.
[0013] Preferably, the seeds in step 1 include but are not limited to wheat seeds, rice seeds, soybean seeds, and tomato seeds.
[0014] Preferably, in step 2, the mixture is rinsed with clean water and then aired to a moisture content of 10%-12%.
[0015] Preferably, the tray in step three is a non-magnetic tray made of polycarbonate.
[0016] Preferably, in step three, the low-frequency electromagnetic generating device adopts a low-frequency alternating magnetic field with a frequency range of 10-100 Hz and a magnetic induction intensity of 1-10 mT.
[0017] Preferably, in step three, the low-frequency electromagnetic generating device includes a coil, a signal generator and a processing chamber. The coil is a Helmholtz coil with a coil diameter of 30 cm and is wound by a copper wire with a diameter of 0.5 mm.
[0018] Preferably, the antioxidant enzyme in step 4 is at least one of superoxide dismutase (SOD) and catalase (CAT).
[0019] Preferably, the change in seed surface temperature in step six should be controlled at ≤2°C.
[0020] Preferably, the seeds after the static treatment in step eight should be sown as soon as possible and stored away from light for no more than 48 hours to avoid a decrease in enzyme activity due to long-term storage.
[0021] Beneficial effects of the present invention:
[0022] This method uses electromagnetic magnetic fields to stimulate enzyme activity inside seeds, and directionally regulates enzyme activity through physical means, replacing traditional chemical mutagenesis and hormone treatment, eliminating the risk of non-target gene mutations and chemical residue pollution, ensuring crop safety and ecological friendliness, while avoiding the interference of exogenous hormones on the seedlings' autonomous adversity response mechanism, and enhancing the plant's stress resistance potential. It targets different target enzymes of amylase and antioxidant enzymes, respectively, and uses characteristic electromagnetic parameters to achieve targeted and precise activation of enzyme activity, significantly optimizes energy metabolism efficiency and antioxidant defense capabilities, promotes rapid seed germination and strong seedling growth, and strictly controls the seed surface temperature difference to ≤2°C through dynamic temperature control and infrared monitoring systems. It also combines the uniform magnetic field design of polycarbonate trays and Helmholtz coils to ensure that the treatment process is efficient and free of thermal damage. This method is not only simple to operate and has wide applicability, supporting the treatment of a variety of crop seeds, but also takes into account production convenience while ensuring enzyme activity, providing green agriculture with a new technology for efficient, safe and controllable seed treatment. DETAILED DESCRIPTION
[0023] The present invention will be further described below with reference to the embodiments.
[0024] Example 1
[0025] A method for stimulating enzyme activity inside seeds through an electromagnetic magnetic field, the preparation steps of which are as follows:
[0026] Step 1: Seed screening: select plump, pest-free, and healthy wheat seeds;
[0027] Step 2: Seed cleaning and disinfection: Use deionized water to carefully wash the seeds to remove surface impurities, then soak the seeds in deionized water at 23°C for 6 hours, then transfer them to 0.1% sodium hypochlorite solution and soak for 10 minutes, then rinse with clean water three times and dry them to a moisture content of 10%;
[0028] Step 3: Seed loading: Spread the pre-treated seeds evenly on a non-magnetic polycarbonate tray, ensuring that the thickness of a single layer does not exceed 2 cm. Then place the tray in the center of the treatment chamber of the low-frequency electromagnetic generator.
[0029] Step 4: Frequency selection: To enhance the activity of amylase, adjust the frequency and magnetic induction intensity of the low-frequency electromagnetic generator to 50 Hz and 5 mT respectively.
[0030] Step 5: Magnetic field treatment: start the equipment, apply an alternating magnetic field, and simultaneously start the temperature control system to maintain a constant temperature environment of 25°C. The treatment time lasts for 20 minutes.
[0031] Step 6: Dynamic monitoring: Use an infrared thermal imager to monitor changes in seed surface temperature to ensure that temperature fluctuations are ≤ 2°C to avoid local overheating;
[0032] Step 7: Static treatment: turn off the magnetic field and place the treated seeds in a dark room for 10 minutes to eliminate residual electromagnetic effects and promote the stability of the enzyme system;
[0033] Step 8: Post-processing. The seeds that have been statically treated can be sown immediately, or stored in the dark at 25°C for no more than 48 hours.
[0034] Example 2
[0035] A method for stimulating enzyme activity inside seeds through an electromagnetic magnetic field, the preparation steps of which are as follows:
[0036] Step 1: Seed screening: select plump, pest-free, and healthy rice seeds;
[0037] Step 2: Seed cleaning and disinfection: Use deionized water to carefully wash the seeds to remove surface impurities, then soak the seeds in deionized water at 23°C for 6 hours, then transfer them to 0.1% sodium hypochlorite solution and soak for 10 minutes, then rinse with clean water three times and dry them to a moisture content of 10%;
[0038] Step 3: Seed loading: Spread the pre-treated seeds evenly on a non-magnetic polycarbonate tray, ensuring that the thickness of a single layer does not exceed 2 cm. Then place the tray in the center of the treatment chamber of the low-frequency electromagnetic generator.
[0039] Step 4: Frequency selection: To increase superoxide dismutase, adjust the frequency and magnetic induction intensity of the low-frequency electromagnetic generator to 75Hz and 8mT respectively.
[0040] Step 5: Magnetic field treatment: start the equipment, apply an alternating magnetic field, and simultaneously start the temperature control system to maintain a constant temperature environment of 25°C. The treatment time lasts for 20 minutes.
[0041] Step 6: Dynamic monitoring: Use an infrared thermal imager to monitor changes in seed surface temperature to ensure that temperature fluctuations are ≤ 2°C to avoid local overheating;
[0042] Step 7: Static treatment: turn off the magnetic field and place the treated seeds in a dark room for 10 minutes to eliminate residual electromagnetic effects and promote the stability of the enzyme system;
[0043] Step 8: Post-processing. The seeds that have been statically treated can be sown immediately, or stored in the dark at 25°C for no more than 48 hours.
[0044] Example 3
[0045] A method for stimulating enzyme activity inside seeds through an electromagnetic magnetic field, the preparation steps of which are as follows:
[0046] Step 1: Seed screening: select plump, pest-free, and healthy soybean seeds;
[0047] Step 2: Seed cleaning and disinfection: Use deionized water to carefully wash the seeds to remove surface impurities, then soak the seeds in deionized water at 24°C for 15 hours, then transfer them to 0.1% sodium hypochlorite solution and soak for 10 minutes, then rinse with clean water three times and dry them to a moisture content of 11%;
[0048] Step 3: Seed loading: Spread the pre-treated seeds evenly on a non-magnetic polycarbonate tray, ensuring that the thickness of a single layer does not exceed 2 cm. Then place the tray in the center of the treatment chamber of the low-frequency electromagnetic generator.
[0049] Step 4: Frequency selection: To enhance the activity of amylase, adjust the frequency and magnetic induction intensity of the low-frequency electromagnetic generator to 50 Hz and 5 mT respectively.
[0050] Step 5: Magnetic field treatment: start the equipment, apply an alternating magnetic field, and simultaneously start the temperature control system to maintain a constant temperature environment of 25°C. The treatment time lasts for 30 minutes.
[0051] Step 6: Dynamic monitoring: Use an infrared thermal imager to monitor changes in seed surface temperature to ensure that temperature fluctuations are ≤ 2°C to avoid local overheating;
[0052] Step 7: Static treatment: turn off the magnetic field and place the treated seeds in a dark room for 10 minutes to eliminate residual electromagnetic effects and promote the stability of the enzyme system;
[0053] Step 8: Post-processing. The seeds that have been statically treated can be sown immediately, or stored in the dark at 25°C for no more than 48 hours.
[0054] Example 4
[0055] A method for stimulating enzyme activity inside seeds through an electromagnetic magnetic field, the preparation steps of which are as follows:
[0056] Step 1: Seed screening, select plump, pest-free, and healthy tomato seeds;
[0057] Step 2: Seed cleaning and disinfection: Use deionized water to carefully wash the seeds to remove surface impurities, then soak the seeds in deionized water at 25°C for 24 hours, then transfer them to 0.1% sodium hypochlorite solution and soak for 10 minutes, then rinse with clean water three times and dry them to a moisture content of 12%;
[0058] Step 3: Seed loading: Spread the pre-treated seeds evenly on a non-magnetic polycarbonate tray, ensuring that the thickness of a single layer does not exceed 2 cm. Then place the tray in the center of the treatment chamber of the low-frequency electromagnetic generator.
[0059] Step 4: Frequency selection: To enhance catalase activity, adjust the frequency and magnetic induction intensity of the low-frequency electromagnetic generator to 75 Hz and 8 mT respectively.
[0060] Step 5: Magnetic field treatment: start the equipment, apply an alternating magnetic field, and simultaneously start the temperature control system to maintain a constant temperature environment of 25°C. The treatment time lasts for 40 minutes.
[0061] Step 6: Dynamic monitoring: Use an infrared thermal imager to monitor changes in seed surface temperature to ensure that temperature fluctuations are ≤ 2°C to avoid local overheating;
[0062] Step 7: Static treatment: turn off the magnetic field and place the treated seeds in a dark room for 10 minutes to eliminate residual electromagnetic effects and promote the stability of the enzyme system;
[0063] Step 8: Post-processing. The seeds that have been statically treated can be sown immediately, or stored in the dark at 25°C for no more than 48 hours.
[0064] The enzyme activities of different crop seeds prepared by electromagnetic magnetic field treatment in the examples, including wheat, rice, soybean, and tomato seeds, were significantly improved. In wheat and soybean seeds, after treatment with 50Hz / 5mT parameters, amylase activity was significantly enhanced, accelerating the decomposition of starch into soluble sugars, providing sufficient energy for seed germination, and the germination rate was increased by 15%-22% compared with the germination rate of the original seeds. After rice and tomato seeds were treated with 75Hz / 8mT, the activities of superoxide dismutase (SOD) and catalase (CAT) were increased by 25%-30%, respectively, effectively removing reactive oxygen and enhancing the antioxidant capacity of seedlings. The survival rate under drought or salt stress conditions was increased by 18%-25%, and the enzyme activity stability was excellent.
[0065] The embodiments of the present invention have been described in detail above, but the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims
1. A method for stimulating enzyme activity in seeds by using an electromagnetic magnetic field, characterized in that: The preparation steps are as follows: Step 1: Seed screening, select healthy seeds that are plump, free of disease and insect pests; Step 2: Clean and disinfect the seeds. Wash the seeds carefully with deionized water to remove surface impurities. Then soak the seeds in deionized water at 23-25°C for 6-24 hours. Then transfer them to 0.1% sodium hypochlorite solution and soak for 10 minutes. Then rinse them with clean water three times and dry them for later use. Step 3: Seed loading: Spread the pre-treated seeds evenly on the tray, ensuring that the thickness of the single layer does not exceed 2 cm. Then place the tray in the center of the treatment chamber of the low-frequency electromagnetic generator. Step 4: Frequency selection. According to the need to enhance the activity of the target enzyme, a specific frequency and magnetic induction intensity are selected. If the target enzyme is amylase, a low-frequency magnetic field of 50 Hz and a magnetic induction intensity of 5 mT should be selected. If the target enzyme is an antioxidant enzyme, a medium-frequency magnetic field of 75 Hz and a magnetic induction intensity of 8 mT should be selected. Step 5: Magnetic field treatment: start the equipment, apply an alternating magnetic field, and simultaneously start the temperature control system to maintain a constant temperature environment of 25°C. The treatment time lasts for 20-40 minutes. Step 6: Dynamic monitoring: Use an infrared thermal imager to monitor changes in seed surface temperature to ensure there is no local overheating; Step 7: Static treatment: turn off the magnetic field and place the treated seeds in a dark room for 10 minutes to eliminate residual electromagnetic effects and promote the stability of the enzyme system; Step 8: Post-treatment: The seeds that have been statically treated can be sown immediately or stored in the dark at 25°C.
2. The method of stimulating enzyme activity in seeds by electromagnetic magnetic field according to claim 1, characterized in that: The seeds in step 1 include but are not limited to wheat seeds, rice seeds, soybean seeds, and tomato seeds.
3. The method of stimulating enzyme activity in seeds by electromagnetic magnetic field according to claim 1, characterized in that: After rinsing with clean water in step 2, the cloth is dried to a moisture content of 10%-12%.
4. The method of stimulating enzyme activity in seeds by electromagnetic magnetic field according to claim 1, characterized in that: The tray in step 3 is a non-magnetic tray made of polycarbonate.
5. The method of stimulating enzyme activity in seeds by electromagnetic magnetic field according to claim 1, characterized in that: In step three, the low-frequency electromagnetic generating device adopts a low-frequency alternating magnetic field with a frequency range of 10-100 Hz and a magnetic induction intensity of 1-10 mT.
6. The method of stimulating enzyme activity in seeds by electromagnetic magnetic field according to claim 1, characterized in that: In step three, the low-frequency electromagnetic generating device includes a coil, a signal generator and a processing chamber. The coil is a Helmholtz coil with a coil diameter of 30 cm and is wound by a copper wire with a diameter of 0.5 mm.
7. The method of stimulating enzyme activity in seeds by using an electromagnetic magnetic field according to claim 1, characterized in that: In step 4, the antioxidant enzyme is at least one of superoxide dismutase and catalase.
8. The method of stimulating enzyme activity in seeds by using an electromagnetic magnetic field according to claim 1, characterized in that: The change in seed surface temperature in step 6 should be controlled at ≤2°C.
9. The method of stimulating enzyme activity in seeds by using an electromagnetic magnetic field according to claim 1, characterized in that: The seeds after the static treatment in step 8 should be sown as soon as possible and stored away from light for no more than 48 hours to avoid a decrease in enzyme activity due to long-term storage.