Application of sialic acid in preparation of medicine for promoting stem cell migration

Abstract

The invention relates to the technical field of medicines, in particular to application of sialic acid in preparation of a medicine for promoting stem cell migration, the administration concentration of the sialic acid is 0.625 mM to 5mM, the sialic acid (SA) has the influence on proliferation and migration of amniotic fluid mesenchymal stem cells (AFMSCs), the sialic acid with the concentration of 0.625 mM, 1.25 mM, 2.5 mM and 5mM is used for experiments, and results show that the sialic acid with the concentration of 0.625 mM, 1.25 mM, 2.5 mM and 5mM has the influence on the proliferation and migration of the AFMSCs. The migration of the amniotic fluid mesenchymal stem cells is promoted, and the migration rate of the AFMSCs is 100% due to the SA with the concentration of 1.25 mM, 2.5 mM and 5 mM in 48 hours.

Description

Technical Field

[0001] The present invention relates to the field of medical technology, and in particular to the application of swollen acid in the preparation of a drug for promoting stem cell migration. Background Art

[0002] Sialic acid (SA), also known as sialic acid, has been found in nature in more than 50 forms. The most important form is N-acetylneuraminic acid. SA is naturally present in many animal foods, such as bird's nests, breast milk and eggs. Sialic acid has the effects of promoting brain development, anti-virus, anti-Alzheimer's disease and anti-hypertension. In addition, SA also has anti-aging, anti-oxidation and whitening effects.

[0003] Stem cells are a core tool in regenerative medicine. Amniotic fluid mesenchymal stem cells (AFMSCs) have multipotential differentiation potential, capable of differentiating into cells such as fat, bone, and cartilage. They are also low immunogenic and have a low risk of allogeneic transplant rejection. AFMSGs are obtained from prenatal amniotic fluid and are ethically uncontroversial. Stem cell therapy has gained significant attention in recent years, but issues such as low homing efficiency and poor microenvironmental adaptability persist. Improving stem cells' ability to migrate, enhancing their adaptability to the environment, and prolonging their duration of action are key to addressing these challenges. Summary of the Invention

[0004] In order to solve the above technical problems, an embodiment of the present invention provides the use of swallow acid in the preparation of a drug for promoting stem cell migration.

[0005] To achieve the above objectives, the embodiments of the present invention adopt the following technical solutions:

[0006] The present invention provides application of swalnic acid in preparing a medicine for promoting stem cell migration.

[0007] In some embodiments, the administration concentration of sialic acid is 0.625 mM to 5 mM.

[0008] In some embodiments, the stem cells are amniotic fluid mesenchymal stem cells.

[0009] In some embodiments, the above-mentioned drug comprises a pharmaceutical preparation prepared with an effective amount of sialic acid as the active ingredient and a pharmaceutically acceptable carrier and / or excipient.

[0010] An effective amount refers to the amount of a drug, compound, or pharmaceutical composition necessary to obtain any one or more beneficial or desired therapeutic results. Beneficial or desired results include: clinical improvement (such as reducing morbidity, reducing mortality, improving one or more symptoms), reducing severity, delaying the onset of symptoms (including symptoms or their complications, intermediate pathological phenotypes presented during the development of the disease, biochemistry, histology, and / or behavioral symptoms). The preparation can be an injection, spray, aerosol, nasal drops, oral preparation, or a dosage form suitable for mucosal administration. In addition, the route of administration to the subject includes, but is not limited to, intramuscular, intravenous, subcutaneous, intradermal, oral, intranasal, respiratory, transmucosal, sublingual, and parenteral.

[0011] The present invention has the following beneficial effects compared to the prior art:

[0012] Bird's nest acid promotes the proliferation of amniotic fluid mesenchymal stem cells by enhancing their migration ability. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a bright field microscope image of amniotic fluid mesenchymal stem cells (AFMSCs);

[0014] Figure 2 This is a physical picture of Sialic acid powder (SA);

[0015] Figure 3 The cell state diagram at 0h;

[0016] Figure 4 This is the cell status diagram at 24 hours;

[0017] Figure 5 This is the cell status diagram at 48h;

[0018] Figure 6 This is a statistical analysis chart of the cell scratch experiment. DETAILED DESCRIPTION

[0019] The following will be combined with the accompanying drawings to clearly and completely describe the technical solutions in some embodiments of the present disclosure. Obviously, the embodiments described are only some embodiments of the present disclosure, not all embodiments. Based on the embodiments provided by the present disclosure, all other embodiments obtained by ordinary technicians in this field are within the scope of protection of the present disclosure.

[0020] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this application can be purchased from the market or prepared by existing methods.

[0021] Experimental Materials:

[0022] (1) Cells

[0023] Amniotic fluid mesenchymal stem cells (AFMSCs) were derived from clinical amniotic fluid samples from the Third Affiliated Hospital of Guangzhou Medical University.

[0024] (2) Materials

[0025] Swallow acid (SA): purchased from MCE, 100 mg, CAS No.: 131-48-6.

[0026] DMEM culture medium: 1% fetal bovine serum + 1% P / S double antibody (final concentration of 50 U / mL) + 98% low-glucose DMEM basal culture medium.

[0027] Example

[0028] Dissolve 100 mg of sialic acid powder in pure water and filter through a 0.22 μm disposable microporous membrane filter to remove impurities to prepare a 32 mM sialic acid solution. Dilute the 32 mM sialic acid solution with DMEM medium to prepare sialic acid solutions with concentrations of 0.625 mM, 1.25 mM, 2.5 mM, and 5 mM, respectively.

[0029] Place 10 5 Amniotic fluid-derived mesenchymal stem cells were evenly plated in a six-well plate. When the cell density reached 90%, the wells of the six-well plate were scratched with a 200μL pipette tip, maintaining a 1cm width per well. 2mL of 0.625mM, 1.25mM, 2.5mM, and 5mM scutellariae were added to the six-well plate and incubated in DMEM basal medium at 37°C and 5% CO2. A control group was maintained without scutellariae solution, i.e., with a 0mM scutellariae solution concentration.

[0030] like Figure 3 The cells were in the state of 0 h. At this time, a 1 cm wide scratch was made with a 200 μL pipette tip, and different concentrations of swine acid solution were added. The cells were cultured at a temperature of 37°C and 5% CO2.

[0031] like Figure 4 The cells have been cultured in the medium containing sialic acid for 24 hours. The cell density has changed, and the scratch width has also narrowed. Compared with the control group (sialic acid concentration of 0mM), the cell density in the medium containing sialic acid has increased and the migration ability has increased.

[0032] like Figure 5The cells were cultured in the culture medium containing sialic acid for 48 hours. The cell density changed significantly and the scratch width became narrower. Compared with the control group (sialic acid concentration of 0 mM), the cell density in the culture medium containing sialic acid almost reached 100%, and the migrating cells filled the gap in the scratch.

[0033] like Figure 6 As shown in the figure, the changes in the width of the scratches were statistically analyzed, and the results showed that compared with the control group (the concentration of 0 mM sialic acid), as the concentration of sialic acid increased, the cell proliferation rate became faster and the cell migration ability increased.

[0034] The effect of sialic acid (SA) on the proliferation and migration of amniotic fluid mesenchymal stem cells (AFMSCs) was investigated through cell scratch experiments. Experiments were conducted with sialic acid at concentrations of 0.625mM, 1.25mM, 2.5mM, and 5mM. The results showed that as the concentration of SA increased, the migration of amniotic fluid mesenchymal stem cells was promoted. At 48h, the migration rate of AFMSCs was 100% when the concentrations of SA were 1.25mM, 2.5mM, and 5mM.

[0035] To sum up, it can be concluded that bird's nest acid enhances the ability of amniotic fluid mesenchymal stem cells to migrate and better promotes the proliferation of amniotic fluid mesenchymal stem cells.

[0036] In the description of this specification, specific features, structures, materials or characteristics may be combined in an appropriate manner in any one or more embodiments or examples.

[0037] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. Application of sialic acid in the preparation of drugs that promote stem cell migration.

2. The use according to claim 1, characterized in that The administration concentration of the sialic acid is 0.625 mM to 5 mM.

3. The use according to claim 1, characterized in that The stem cells are amniotic fluid mesenchymal stem cells.

Images