Traditional Chinese medicine composition for treating inadventitious heart and deficiency of qi and blood as well as extract and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for mental disorder and deficiency of qi and blood as well as an extract and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following medicinal materials in parts by weight: 3-30 parts of poria with hostwood, 5-40 parts of codonopsis pilosula, 3-50 parts of ophiopogon roots, 6-45 parts of polygala tenuifolia, 5-40 parts of platycodon grandiflorum, 3-30 parts of asparagus fern, 4-50 parts of platycladi seeds, 5-60 parts of acorus calamus, 3-40 parts of schisandra chinensis, 6-45 parts of radix rehmanniae recen, 7-40 parts of prepared rehmannia roots, 5-60 parts of scrophularia ningpoensis, 3-40 parts of fried jujube kernels and 5-45 parts of moutan bark. By adopting the formula of the traditional Chinese medicine composition, qi deficiency and blood deficiency can be improved at the same time, the improvement effect is good, and the traditional Chinese medicine composition can be used for improving symptoms such as inadventitious heart and deficiency of both qi and blood.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for treating restlessness and qi and blood deficiency, as well as an extract and use thereof. Background Art

[0002] As the pace of life accelerates and pressure increases, deficiency of qi and blood has become a typical symptom of sub-healthy people (such as those who stay up late for a long time, postpartum women, and postoperative patients), manifested as palpitations and insomnia, fatigue and dizziness, sallow complexion, etc. Western medicine often uses sedatives and tranquilizers in combination with iron / vitamins for treatment, but there are side effects such as drowsiness dependence and gastrointestinal irritation, and it is impossible to regulate the balance of qi and blood as a whole. Existing drugs are mostly single qi-tonifying or blood-tonifying, or the qi-tonifying effect is good but the blood-tonifying effect is general, or the blood-tonifying effect is good but the qi-tonifying effect is general. Therefore, it is of great significance to develop a Chinese medicine composition that takes into account both qi-tonifying and blood-tonifying, and improves qi deficiency and blood deficiency at the same time. Summary of the Invention

[0003] In view of this, the present invention provides a traditional Chinese medicine composition for restlessness and deficiency of qi and blood, as well as an extract and use thereof.

[0004] The technical solution of the present invention is achieved as follows: a traditional Chinese medicine composition for treating deficiency of both qi and blood, comprising the following medicinal materials in parts by weight: 3-30 parts of Poria cocos, 5-40 parts of Codonopsis pilosula, 3-50 parts of Ophiopogon japonicus, 6-45 parts of Polygala tenuifolia, 5-40 parts of Platycodon grandiflorum, 3-30 parts of Asparagus cochinchinensis, 4-50 parts of Platycladi seed, 5-60 parts of Acorus calamus, 3-40 parts of Schisandra chinensis, 6-45 parts of Rehmannia root, 7-40 parts of Rehmannia root prepared, 5-60 parts of Radix Yuanshen, 3-40 parts of stir-fried Ziziphus jujuba seeds, and 5-45 parts of Paeonia suffruticosa.

[0005] Furthermore, the traditional Chinese medicine composition comprises the following medicinal materials in parts by weight: 10-20 parts of Poria cocos, 12-15 parts of Codonopsis pilosula, 10-15 parts of Ophiopogon japonicus, 8-12 parts of Polygala tenuifolia, 10-15 parts of Platycodon grandiflorum, 6-9 parts of Asparagus cochinchinensis, 10-15 parts of Platycladus chinensis seeds, 8-12 parts of Acorus calamus, 6-9 parts of Schisandra chinensis, 15-23 parts of Rehmannia root, 15-20 parts of Rehmannia root prepared, 8-12 parts of Radix Yuanshen, 9-12 parts of stir-fried Ziziphus jujuba seeds, and 12-20 parts of Paeonia suffruticosa.

[0006] Furthermore, the Chinese medicine composition includes the following medicinal materials in parts by weight: 15 parts of Poria, 14 parts of Codonopsis, 12 parts of Ophiopogon, 10 parts of Polygala, 15 parts of Platycodon, 7 parts of Asparagus, 12 parts of Platycladus, 10 parts of Acorus, 8 parts of Schisandra, 18 parts of Rehmannia, 17 parts of Rehmannia, 10 parts of Yuanshen, 10 parts of stir-fried Ziziphus jujuba seeds, and 15 parts of Paeonia suffruticosa.

[0007] The method for preparing any one of the Chinese medicine compositions of the present invention comprises the following steps:

[0008] (1) Mix Poria, Codonopsis, Radix Ophiopogonis, Asparagus cochinchinensis, Radix Rehmanniae, Radix Rehmanniae Preparata, Radix Salviae Miltiorrhizae, and Cortex Moutan, add 8-10 times their weight of water, extract at 85-95° C. for 2-3 times, each time for 0.8-1.2 hours, filter, and combine the filtrates;

[0009] (2) Take Polygala tenuifolia, Schisandra chinensis, and Platycodon grandiflorum, add 6-8 times their weight of 65%-75% v / v ethanol solution, reflux extract at 80-85°C for 2-3 times, each time for 1-2 hours, and combine the ethanol extracts;

[0010] (3) taking calamus, cypress seed and fried jujube seed, adding 2-4 times the weight of 45%-55% v / v ethanol solution, and extracting for 20-40 minutes with ultrasonic power of 250-350W and microwave power of 450-550W to obtain an extract;

[0011] (4) The filtrate of step (1) is mixed with the alcohol extract of step (2), and then the extract of step (3) is added and mixed evenly, and concentrated under reduced pressure at a temperature of 55-65° C. to a relative density of 1.10-1.15 to obtain an extract.

[0012] A traditional Chinese medicine composition preparation for treating restlessness and deficiency of qi and blood is prepared from any traditional Chinese medicine composition of the present invention alone or by mixing any traditional Chinese medicine composition of the present invention with a pharmaceutically acceptable carrier.

[0013] Furthermore, the Chinese medicine composition preparation is a decoction and a granule.

[0014] Use of any one of the traditional Chinese medicine compositions of the present invention in preparing products for treating restlessness and deficiency of qi and blood.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) The Chinese medicine composition of the present invention can improve both qi deficiency and blood deficiency at the same time, and the improvement effect is good. It can be used to improve symptoms such as qi and blood deficiency, restlessness, etc.

[0017] (2) In the prescription of the present invention, Codonopsis pilosula, Acorus calamus, Radix Rehmanniae glutinosae and Radix Rehmanniae glutinosae are used as the main drugs. Among them, Codonopsis pilosula replenishes Qi and strengthens the spleen, Acorus calamus regulates Qi and activates blood circulation, Radix Rehmanniae glutinosae nourishes Yin and blood, and Radix Rehmanniae glutinosae replenishes blood and assists Qi. The three form a "spleen-lung-kidney" three-organ coordination network, and synergistically exert the effects of mutual generation of Qi and blood, replenishing Qi and blood, and regulating the mind; Poria, Paeonia suffruticosa, Radix Yuanshen, Platycladi seed, Asparagus cochinchinensis, stir-fried Ziziphus jujuba seed, Radix Ophiopogonis and Radix Ophiopogonis are used as the auxiliary drugs. Among them, Poria calms the mind and soothes the mind. The medicine is as follows: Peony bark promotes blood circulation and dissipates blood stasis, Yuanshen nourishes yin and detoxifies, and pine nut seeds nourish the heart and calm the mind. Poria, Peony bark, Yuanshen, and pine nut seeds work together to nourish blood, replenish qi and calm the mind. Asparagus, stir-fried jujube seeds, and Ophiopogon work together to nourish yin and calm the mind. Schisandra chinensis, Platycodon grandiflorum, and Polygala tenuifolia are used as adjuvants. Among them, Schisandra chinensis astringes the lungs and nourishes the kidneys, Platycodon grandiflorum invigorates and promotes lung qi, and Polygala tenuifolia harmonizes the heart and kidneys. The whole prescription has the effects of replenishing qi, blood, and calming the mind, and is effective in improving qi and blood deficiency and restlessness.

[0018] (3) The extraction process of the present invention is used to fully extract the active ingredients of each medicinal flavor, thereby improving both the yield and bioavailability of the active ingredients and enhancing the efficacy of invigorating qi and nourishing blood. DETAILED DESCRIPTION

[0019] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0020] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0021] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0022] Example 1

[0023] A traditional Chinese medicine composition for treating restlessness and deficiency of both qi and blood, comprising the following medicinal materials in parts by weight: 3 parts of poria, 5 parts of codonopsis pilosula, 3 parts of ophiopogon, 6 parts of polygala, 5 parts of platycodon, 3 parts of asparagus cochinchinensis, 4 parts of cypress seeds, 5 parts of calamus, 3 parts of schisandra chinensis, 6 parts of raw rehmannia, 7 parts of cooked rehmannia, 5 parts of yuanshen, 3 parts of stir-fried jujube seeds, and 5 parts of moutan bark.

[0024] Example 2

[0025] A traditional Chinese medicine composition for treating restlessness and deficiency of both qi and blood, comprising the following medicinal materials in parts by weight: 30 parts of poria, 40 parts of codonopsis pilosula, 50 parts of ophiopogon, 45 parts of polygala, 40 parts of platycodon, 30 parts of asparagus cochinchinensis, 50 parts of cypress seeds, 60 parts of calamus, 40 parts of schisandra chinensis, 45 parts of raw rehmannia root, 40 parts of cooked rehmannia root, 60 parts of yuanshen, 40 parts of stir-fried jujube seeds, and 45 parts of moutan bark.

[0026] Example 3

[0027] A traditional Chinese medicine composition for treating restlessness and deficiency of both qi and blood, comprising the following medicinal materials in parts by weight: 15 parts of poria, 14 parts of codonopsis pilosula, 12 parts of ophiopogon, 10 parts of polygala tenuifolia, 15 parts of platycodon, 7 parts of asparagus cochinchinensis, 12 parts of cypress seed, 10 parts of calamus, 8 parts of schisandra chinensis, 18 parts of raw rehmannia root, 17 parts of cooked rehmannia root, 10 parts of yuanshen, 10 parts of stir-fried jujube seeds, and 15 parts of moutan bark.

[0028] Example 4

[0029] According to Example 1, the medicinal materials were taken, mixed, and 9 times the weight of water was added, and decocted twice, each time for 1.0 hour. After decocting, the filtrates were filtered and combined; and concentrated under reduced pressure at 60° C. to a relative density of 1.15 to obtain an extract.

[0030] Example 5

[0031] The medicinal materials were taken according to Example 2, mixed, and 9 times the weight of water was added, and the mixture was decocted twice, each time for 1.0 hour. After decocting, the mixture was filtered and the filtrates were combined; the mixture was concentrated under reduced pressure at 60° C. to a relative density of 1.15 to obtain an extract.

[0032] Example 6

[0033] The medicinal materials were taken according to Example 3, mixed, and 9 times the weight of water was added, and the mixture was decocted twice, each time for 1.0 hour. After decocting, the mixture was filtered and the filtrates were combined; the mixture was concentrated under reduced pressure at 60° C. to a relative density of 1.15 to obtain an extract.

[0034] Example 7

[0035] A method for preparing a traditional Chinese medicine composition for treating restlessness and qi and blood deficiency, comprising taking the medicinal materials according to the formula of Example 3, and specifically comprising the following steps:

[0036] (1) Mix Poria, Codonopsis, Radix Ophiopogonis, Asparagus cochinchinensis, Radix Rehmanniae, Radix Rehmanniae Preparata, Radix Salviae Miltiorrhizae, and Cortex Moutan, add 9 times the weight of water, extract twice at 90°C for 1 hour each time, filter, and combine the filtrates;

[0037] (2) Take Polygala tenuifolia, Schisandra chinensis, and Platycodon grandiflorum, add 7 times their weight of 70% v / v ethanol solution, reflux extract at 80°C twice, each time for 1.5 hours, and combine the ethanol extracts;

[0038] (3) Add 3 times the weight of 50% v / v ethanol solution to calamus, cypress seed, and fried jujube seed, and extract for 30 minutes using ultrasonic power of 300W and microwave power of 500W to obtain an extract;

[0039] (4) The filtrate of step (1) and the alcohol extract of step (2) were mixed, and then the extract of step (3) was added and mixed evenly, and the mixture was concentrated under reduced pressure at 60° C. to a relative density of 1.15 to obtain an extract.

[0040] Example 8

[0041] A method for preparing a traditional Chinese medicine composition for treating restlessness and qi and blood deficiency, comprising taking the medicinal materials according to the formula of Example 3, and specifically comprising the following steps:

[0042] (1) Mix Poria, Codonopsis, Radix Ophiopogonis, Asparagus cochinchinensis, Radix Rehmanniae, Radix Rehmanniae Preparata, Radix Salviae Miltiorrhizae, and Cortex Moutan, add 8-10 times their weight of water, extract twice at 85°C, each time for 0.8 hours, filter, and combine the filtrates;

[0043] (2) Take Polygala tenuifolia, Schisandra chinensis, and Platycodon grandiflorum, add 6 times their weight of 65% v / v ethanol solution, reflux extract at 80°C twice, each time for 2 hours, and combine the ethanol extracts;

[0044] (3) Add twice the weight of 45% v / v ethanol solution to calamus, cypress seed, and fried jujube seed, and extract for 40 minutes at an ultrasonic power of 250 W and a microwave power of 450 W to obtain an extract;

[0045] (4) The filtrate of step (1) and the alcohol extract of step (2) were mixed, and then the extract of step (3) was added and mixed evenly, and the mixture was concentrated under reduced pressure at 60° C. to a relative density of 1.15 to obtain an extract.

[0046] Example 9

[0047] A method for preparing a traditional Chinese medicine composition for treating restlessness and qi and blood deficiency, comprising taking the medicinal materials according to the formula of Example 3, and specifically comprising the following steps:

[0048] (1) Mix Poria, Codonopsis, Radix Ophiopogonis, Asparagus cochinchinensis, Radix Rehmanniae, Radix Rehmanniae Preparata, Radix Salviae Miltiorrhizae, and Cortex Moutan, add 8-10 times their weight of water, extract at 95°C for 3 times, each time for 1.2 hours, filter, and combine the filtrates;

[0049] (2) Take Polygala tenuifolia, Schisandra chinensis, and Platycodon grandiflorum, add 8 times their weight of 75% v / v ethanol solution, reflux extract at 85°C for 3 times, each time for 1 hour, and combine the ethanol extracts;

[0050] (3) Add 4 times the weight of 55% v / v ethanol solution to calamus, cypress seed, and fried jujube seed, and extract them for 40 minutes at an ultrasonic power of 350 W and a microwave power of 550 W to obtain an extract;

[0051] (4) The filtrate of step (1) and the alcohol extract of step (2) were mixed, and then the extract of step (3) was added and mixed evenly, and the mixture was concentrated under reduced pressure at 60° C. to a relative density of 1.15 to obtain an extract.

[0052] Comparative Example 1

[0053] The difference from Example 6 is that the peony bark is replaced with red peony root. Specifically, a traditional Chinese medicine composition comprises the following medicinal materials in parts by weight: 15 parts of Poria, 14 parts of Codonopsis, 12 parts of Radix Ophiopogonis, 10 parts of Polygala, 15 parts of Platycodon, 7 parts of Asparagus, 12 parts of Platycladi, 10 parts of Acorus, 8 parts of Schisandra, 18 parts of Rehmannia, 17 parts of Rehmannia, 10 parts of Radix Glehniae, 10 parts of Radix Ziziphi Spinosae, 10 parts of stir-fried Ziziphus jujuba seeds, and 15 parts of red peony root.

[0054] Comparative Example 2

[0055] The difference from Example 6 is that Poria is replaced by Poria. Specifically, a Chinese medicine composition comprises the following medicinal materials in parts by weight: 15 parts of Poria, 14 parts of Codonopsis, 12 parts of Radix Ophiopogonis, 10 parts of Polygala, 15 parts of Platycodon, 7 parts of Asparagus, 12 parts of Platycladi, 10 parts of Acorus, 8 parts of Schisandra, 18 parts of Rehmannia, 17 parts of Rehmannia, 10 parts of Radix Yuanshen, 10 parts of stir-fried Ziziphus jujuba seeds, and 15 parts of Paeonia suffruticosa.

[0056] Comparative Example 3

[0057] The difference from Example 6 is that only raw Rehmannia root is used, and cooked Rehmannia root is replaced by raw Rehmannia root. Specifically, a Chinese medicine composition includes the following medicinal materials in parts by weight: 15 parts of Poria cocos, 14 parts of Codonopsis pilosula, 12 parts of Ophiopogon japonicus, 10 parts of Polygala tenuifolia, 15 parts of Platycodon grandiflorum, 7 parts of Asparagus cochinchinensis, 12 parts of Platycladus orientalis seeds, 10 parts of Acorus calamus, 8 parts of Schisandra chinensis, 35 parts of raw Rehmannia root, 10 parts of Radix Yuanshen, 10 parts of stir-fried Ziziphus jujuba seeds, and 15 parts of Paeonia suffruticosa.

[0058] Comparative Example 4

[0059] The difference from Example 7 is that the reflux extraction solvent is 85% v / v ethanol solution.

[0060] Specifically: (2) Take Polygala tenuifolia, Schisandra chinensis and Platycodon grandiflorum, add 7 times their weight of 85% v / v ethanol solution, reflux extract at 80°C twice, each time for 1.5 hours, and combine the ethanol extracts. Other steps are the same as in Example 7.

[0061] Comparative Example 5

[0062] The difference from Example 7 is that the combined extraction solvent is 75% v / v ethanol solution.

[0063] Specifically: (3) taking calamus, cypress seed and fried jujube seed, adding 3 times the weight of 75% v / v ethanol solution, and extracting for 30 minutes at an ultrasonic power of 300W and a microwave power of 500W to obtain an extract. The other steps are the same as those in Example 7.

[0064] Test Example 1

[0065] 1.1 Construction of Qi Deficiency Model and Validation of Drug Efficacy

[0066] 1.1.1 Experimental Animal Preparation: Select zebrafish embryos (AB wild type) with normal development at 2-3 dpf and randomly place them into 12-well plates, with 10-15 zebrafish placed in each well.

[0067] 1.1.2 Drug: The extracts obtained in Examples 4-7 and Comparative Examples 1-5 were diluted with 10 times the weight of purified water to obtain a drug solution.

[0068] 1.1.3 Solution:

[0069] (1) Zebrafish embryo culture medium: Weigh 1463 mg of sodium chloride, 243 mg of calcium chloride, 63 mg of potassium chloride, and 198 mg of magnesium sulfate, dilute with purified water, sonicate, and transfer to a volumetric flask (500 mL) after all are dissolved. Adjust the volume to obtain 10X zebrafish embryo culture medium, and store at low temperature (4°C). When using, dilute to 1X with purified water and preheat to about 28.5°C. Adjust the pH value to about 7.2 with NaHCO3 solution. The salinity range of the culture medium is 0.25% to 0.5%. Add 1-2 drops of methylene blue solution per liter when using.

[0070] (2) Vinorelbine solution (100 μg / mL): Accurately weigh the vinorelbine standard and dilute it with DMSO to obtain a 100 mg / mL vinorelbine stock solution. Dilute it to 100 μg / mL with zebrafish embryo culture medium (DMSO content is 0.1%). Store at low temperature (4°C) and preheat to about 28.5°C before use.

[0071] (3) DMSO culture medium (0.1%): Dimethyl sulfoxide (DMSO) was measured and diluted with zebrafish embryo culture medium to a volume percentage of 0.1%, thereby obtaining 0.1% DMSO culture medium. The solution was stored at a low temperature (4°C) and preheated to about 28.5°C before use.

[0072] (4) Anesthetic solution: Weigh 3-ethoxyanilide methanesulfonate (tricaine animal anesthetic), dilute it with zebrafish embryo culture medium to obtain an anesthetic solution with a mass concentration of 0.5%, and store it at low temperature (4°C).

[0073] 1.1.4 Grouping and Dosage Treatment: A comparative efficacy experiment was conducted, and the experiment was divided into study groups 1-9 (Examples 4-7 and Comparative Examples 1-5), a model group, and a blank group. The blank group was given zebrafish embryo culture medium, and the remaining groups were given vinorelbine solution (100 μg / mL). Each group was set up with 5 replicates, and the dosing volume per well was 3.0 mL. Placed in an incubator, reacted for 24 hours, and a Qi deficiency model was constructed.

[0074] 1.1.5 Subsequent intervention: After the reaction, the blank group and the model group were replaced with the corresponding solution. The remaining groups were first washed three times with zebrafish embryo culture medium, then replaced with the corresponding drug, and then placed in the incubator for 12 hours of drug intervention.

[0075] 1.2 Neutrophil fluorescence intensity detection

[0076] Drug washing: After the drug intervention, wash three times with zebrafish embryo culture medium to remove residual drugs.

[0077] Anesthesia and Slide Preparation: Pipette the zebrafish into the anesthetic solution and let it rest for 3-5 minutes. Once the zebrafish is anesthetized, remove the larvae and place them on a biconcave glass slide in an upright position.

[0078] Image acquisition and cell counting: Place the slide under a fluorescence microscope to acquire images of the entire fish. Use ImageJ image processing software to count neutrophils in the region from the end of the yolk sac to the tail.

[0079] Calculate the percentage increase in the average number of neutrophils = (average number of neutrophils in the study group - average number of neutrophils in the model group) / average number of neutrophils in the model group * 100%

[0080] 1.3 Test results

[0081] Table 1 Comparison of Qi-invigorating effects

[0082] Grouping Average number of neutrophils Average neutrophil count increase Blank group 105 -- Model Group 55 -- Example 4 81 47.3％ Example 5 83 50.9％ Example 6 86 56.4％ Example 7 98 78.2％ Comparative Example 1 72 30.9％ Comparative Example 2 70 27.3％ Comparative Example 3 74 34.5％ Comparative Example 4 90 63.6％ Comparative Example 5 91 65.5％

[0083] The results showed that compared with the model group, the average number of neutrophils in Examples 4-7 and Comparative Examples 4-5 increased significantly (p < 0.001), and the average number of neutrophils tended to be normal, indicating that the composition of the present invention has a significant improvement effect on Qi deficiency. However, the effect of Comparative Examples 1-3 on improving Qi deficiency was poor. Compared with Example 7, the effect of Comparative Examples 4-5 on improving Qi deficiency decreased.

[0084] Test Example 2

[0085] 2.1 Anemia model construction and drug intervention

[0086] 2.1.1 Zebrafish selection and grouping: Normally developed zebrafish embryos at 2-3 dpf were selected and randomly placed in 12-well plates, with 10-15 embryos per well.

[0087] 2.1.2 Medicine: The extracts obtained in Examples 4-7 and Comparative Examples 1-5 were diluted with 10 times the weight of purified water to obtain a medicinal solution.

[0088] 2.1.3 Efficacy Verification Model Construction: A blank group, a model group, study groups 1-9 (Examples 4-7 and Comparative Examples 1-5), and a positive control drug (ferrous succinate tablets) group were set up. The blank group was given zebrafish embryo culture medium, and the remaining groups were given a 0.01 μg / mL phenylhydrazine solution. Each group was set up with 5 replicate wells, with a dosage of 3.0 mL per well, and placed in an incubator for 12 hours to construct an anemia model.

[0089] 2.1.4 Drug intervention: After the reaction, the blank group was replaced with the corresponding solution, and the other groups were first washed three times with zebrafish embryo culture medium, and then replaced with the corresponding drugs, and continued to be treated with drugs in the incubator for 24 hours.

[0090] 2.1.5 Cardiac red blood cell area detection

[0091] Drug washing: After drug intervention, the cells were washed three times with zebrafish embryo culture medium to remove residual drugs.

[0092] Staining and washing: Use o-dianisidine to stain in the dark for 15 minutes, then wash three times with DMSO to remove the stain.

[0093] Image acquisition: The zebrafish was placed in the anteroposterior position on a biconcave glass slide and placed under a fluorescence microscope to acquire whole-fish images.

[0094] Area processing: ImageJ image processing software was used to analyze and process the cardiac red blood cell staining area.

[0095] Calculate the percentage increase in cardiac red blood cell area = (cardiac red blood cell area of the study group - cardiac red blood cell area of the model group) / cardiac red blood cell area of the model group * 100%

[0096] 2.1.6 Model Construction Results

[0097] Statistical results showed that compared with the blank group, the red blood cell area in the zebrafish heart of the model group was significantly reduced (P<0.0001); 24 hours after positive drug treatment, the red blood cell area in the zebrafish heart was significantly higher than that in the model group (P<0.0001). Treatment of zebrafish embryos with 0.01 μg / mL phenylhydrazine did not cause deformities or death, successfully establishing a zebrafish anemia model.

[0098] 2.2 Test results

[0099] Table 2 Comparison of blood-enriching effects

[0100] Grouping Cardiac red blood cell area Increased percentage of cardiac red blood cell area Blank group 8850.6 -- Model Group 5615.9 -- Example 4 8228.1 46.5％ Example 5 8194.5 45.9％ Example 6 8420.3 49.9％ Example 7 8727.7 55.4％ Comparative Example 1 7298.1 30.0％ Comparative Example 2 7458.1 32.8％ Comparative Example 3 7908.8 40.8％ Comparative Example 4 8480.9 51.0％ Comparative Example 5 8541.5 52.1％

[0101] The results show that compared with the model group, the cardiac erythrocyte area of Examples 4-7 and Comparative Examples 4-5 significantly increased (p < 0.001), and the cardiac erythrocyte area tended to be normal, indicating that the present composition has a significant improvement effect on blood deficiency. However, the effect of Comparative Examples 1-3 on improving blood deficiency is not ideal. Compared with Example 7, the effect of Comparative Examples 4-5 on improving blood deficiency also decreased.

[0102] In summary, the Chinese medicine composition of the present invention can improve qi deficiency and blood deficiency at the same time, and has good effects of replenishing qi and blood.

[0103] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the scope of the present invention should be included in the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for treating Qi and blood deficiency, characterized in that: The invention comprises the following medicinal materials in parts by weight: 3-30 parts of Poria cocos, 5-40 parts of Codonopsis pilosula, 3-50 parts of Ophiopogon japonicus, 6-45 parts of Polygala tenuifolia, 5-40 parts of Platycodon grandiflorum, 3-30 parts of Asparagus cochinchinensis, 4-50 parts of Platycladus chinensis seeds, 5-60 parts of Acorus calamus, 3-40 parts of Schisandra chinensis, 6-45 parts of Rehmannia root, 7-40 parts of Rehmannia root prepared, 5-60 parts of Radix Yuanshen, 3-40 parts of stir-fried Ziziphus jujuba seeds, and 5-45 parts of Paeonia suffruticosa.

2. The Chinese medicine composition according to claim 1, characterized in that The invention comprises the following medicinal materials in parts by weight: 10-20 parts of Poria cocos, 12-15 parts of Codonopsis pilosula, 10-15 parts of Ophiopogon japonicus, 8-12 parts of Polygala tenuifolia, 10-15 parts of Platycodon grandiflorum, 6-9 parts of Asparagus cochinchinensis, 10-15 parts of Platycladus chinensis seeds, 8-12 parts of Acorus calamus, 6-9 parts of Schisandra chinensis, 15-23 parts of Rehmannia root, 15-20 parts of Rehmannia root prepared, 8-12 parts of Yuanshen, 9-12 parts of stir-fried Ziziphus jujuba seeds, and 12-20 parts of Paeonia suffruticosa bark.

3. The Chinese medicine composition according to claim 2, characterized in that The invention comprises the following medicinal materials in parts by weight: 15 parts of Poria cocos, 14 parts of Codonopsis pilosula, 12 parts of Ophiopogon japonicus, 10 parts of Polygala tenuifolia, 15 parts of Platycodon grandiflorum, 7 parts of Asparagus cochinchinensis, 12 parts of Platycladus chinensis seeds, 10 parts of Acorus calamus, 8 parts of Schisandra chinensis, 18 parts of Rehmannia root, 17 parts of Rehmannia root prepared, 10 parts of Yuanshen, 10 parts of stir-fried Ziziphus jujuba seeds, and 15 parts of Paeonia suffruticosa.

4. A Chinese herbal extract for treating Qi and blood deficiency, characterized in that: Extraction using the raw material drug in the traditional Chinese medicine composition according to any one of claims 1 to 3 comprises the following steps: (1) Mix Poria, Codonopsis, Radix Ophiopogonis, Asparagus cochinchinensis, Radix Rehmanniae, Radix Rehmanniae Preparata, Radix Salviae Miltiorrhizae, and Cortex Moutan, add 8-10 times their weight of water, extract at 85-95° C. for 2-3 times, each time for 0.8-1.2 hours, filter, and combine the filtrates; (2) Take Polygala tenuifolia, Schisandra chinensis, and Platycodon grandiflorum, add 6-8 times their weight of 65%-75% v / v ethanol solution, reflux extract at 80-85°C for 2-3 times, each time for 1-2 hours, and combine the ethanol extracts; (3) taking calamus, cypress seed and fried jujube seed, adding 2-4 times the weight of 45%-55% v / v ethanol solution, and extracting for 20-40 minutes with ultrasonic power of 250-350W and microwave power of 450-550W to obtain an extract; (4) The filtrate of step (1) is mixed with the alcohol extract of step (2), and then the extract of step (3) is added and mixed evenly, and concentrated under reduced pressure at a temperature of 55-65° C. to a relative density of 1.10-1.15 to obtain an extract.

5. A Chinese medicine composition preparation for treating Qi and blood deficiency, characterized in that: The invention is prepared by the Chinese medicine composition according to any one of claims 1 to 3 alone or by mixing the Chinese medicine composition according to any one of claims 1 to 3 with a pharmaceutically acceptable carrier.

6. The Chinese medicine composition preparation according to claim 5, characterized in that The Chinese medicine composition preparation is decoction and granule.

7. Use of the traditional Chinese medicine composition according to any one of claims 1 to 3 or the traditional Chinese medicine extract according to claim 4 in the preparation of products for treating restlessness and deficiency of qi and blood.