Strain concentration culture monitoring system
By separating the culture chamber in the biological incubator and using a sealed cover, solenoid valve and sampling mechanism, the complex problems of infection and sampling in multi-strain culture are solved, and the individual culture of the strain and precise concentration detection are achieved.
Patent Information
- Application Number
- CN202510399160.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-01
- Publication Date
- 2025-08-19
AI Technical Summary
During the strain culture process, it is difficult for the prior art to achieve separate culture and precise concentration detection of multiple strains, and there are problems of complex strain infection and sampling operations.
The biological incubator is used to separate it into a spare chamber and multiple culture chambers. The gas control is performed using a sealed cover and a three-way solenoid valve. Combined with the lift rack and the sampling mechanism, the sealed culture and automatic sampling of the strain are achieved, the strain is avoided infection, and accurate sampling is achieved through the capillary.
The separate culture of multiple strains was achieved, which reduced the risk of strain infection, simplified sampling operations, and improved the accuracy and efficiency of concentration detection.
Smart Images
Figure CN120505181A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of biological culture detection, in particular to a strain concentration culture monitoring system. Background Art
[0002] Measuring strain concentration during bacterial culture is a core step in both microbiological experiments and industrial production. In microbiological experiments, it monitors growth dynamics while ensuring experimental standardization and reproducibility. In industry, this approach impacts the efficiency, cost, and quality of the entire fermentation process. Different methods are used for strain concentration testing, such as optical measurement, cell counting, and plate counting.
[0003] During the laboratory cultivation of microbial strains, most methods for detecting strain concentration require sampling, followed by dilution to an appropriate concentration. However, laboratory cultivation of strains often uses culture containers called petri dishes. To control the microbial growth environment, such as carbon dioxide concentration, the entire dish is directly exposed to the incubator. This method can only cultivate one type of fungus within a single incubator, thereby preventing cross-infection between different strains.
[0004] If multiple cultures need to be cultured in a single incubator, it is necessary to consider capping different culture dishes. In this case, if the culture dishes need to be sampled, in order to measure the strain concentration. However, once opened, there is still a risk of infection by external bacteria, which is not conducive to the continued cultivation of subsequent strains. Moreover, if the strains are cultured in batches, such as liquid culture media, especially in the form of several types of fungi and dozens of culture media for a single fungus, if samples are taken manually one by one, the burden of sampling will be directly increased, and it is easy to cause confusion of samples, which is not conducive to the accuracy of sampling and even subsequent experiments. Summary of the Invention
[0005] The object of the present invention is to provide a strain concentration culture monitoring system to solve the problems raised in the above background technology.
[0006] To achieve the above objectives, the present invention provides the following technical solutions: a strain concentration culture monitoring system, comprising a biological incubator, wherein a chamber is provided inside the biological incubator, and a sealed door is installed on the front side of the chamber, a control panel is provided on one side of the biological incubator, a signal transceiver is provided on the top of the biological incubator, a partition is provided at the bottom of the chamber of the biological incubator, and the partition divides the chamber from top to bottom into a culture chamber and a spare chamber, and a plurality of air supply partitions are provided inside the culture chamber from top to bottom, and the air supply partitions divide the culture chamber into a plurality of culture chambers; Guide racks are installed on both side walls of the culture chambers, and a receiving tray is slidably connected between the side walls of the left and right guide racks. Culture bottles are installed on the surface of the receiving tray through a plurality of through-holes. Extension boxes are installed inside the biological incubators on one side of the culture chamber. Lifting racks are installed inside the extension boxes via a power mechanism. Several transmission shafts are installed at the bottom of the lifting racks via bearings. A sealing cover is detachably installed at the bottom of the transmission shaft. The sealing cover is used in conjunction with the culture bottle. A three-way solenoid valve is installed on the top of the sealing cover, and one end of the three-way solenoid valve extends to the bottom of the sealing cover. Several trachea are installed on the top of the inner wall of the culture chambers. The tops of the left and right guide racks are slidably connected to the sampling rack, and a sampling mechanism is connected between the surface of the sampling rack and the three-way electromagnetic valve.
[0007] Preferably, the sealing door surface on the front side of the culture chamber is installed with a split observation door.
[0008] Preferably, a synchronous slide is installed inside the biological culture box on one side of the culture cavity. The synchronous slide and the extension box are relatively distributed on the left and right, and the end of the lifting frame is connected to the synchronous slide via a slider.
[0009] Preferably, the power mechanism includes a first electric push rod, a second electric push rod, a rack and a gear, the second electric push rod is installed at the bottom end of the inner wall of the extension box, the output rod of the second electric push rod extends vertically upward, the first electric push rod is installed at the end of the output rod of the second electric push rod, the output rod of the first electric push rod extends toward the direction of the synchronous slide groove, the end of the output rod of the first electric push rod is installed with a rack, a gear is installed on the surface of the transmission shaft, the gear is engaged with the rack, and the top of the shell of the first electric push rod is connected to the lifting frame.
[0010] Preferably, the three-way solenoid valve is provided with two output ends and one input end, the input end is connected to the air pipe, one output end extends to the bottom of the sealing cover and is connected to a multi-purpose tube, and the other output end is installed with a temporary storage cylinder.
[0011] Preferably, the sampling mechanism includes a unit assembly, a capillary tube and an electromagnetic flow sensor. The electromagnetic flow sensor is installed on the temporary storage cylinder, and the discharge port of the temporary storage cylinder is plugged with a capillary tube. The unit assembly is installed between the capillary tube and the sampling rack.
[0012] Preferably, the unit assembly includes a type of unit capsule and a limiting seat. Several limiting seats are provided on the surface of the sampling rack. The top and one side of the limiting seat are both opened. A type of unit capsule is installed on the limiting seat through the side opening. An injection port is provided on the side surface of the type of unit capsule, and the injection port is detachably connected to the capillary.
[0013] Preferably, several of the limiting seats are arranged adjacent to each other, and the second type of unit capsules are installed on several adjacent limiting seats, and the second type of unit capsules are composed of several first type unit capsules interconnected through pipes.
[0014] Preferably, a seal is provided in the bottom inner cavity of the sealing cover, a sleeve shaft is detachably mounted on the bottom end of the transmission shaft, the bottom end of the sleeve shaft extends below the sealing cover, and a stirring rod is sleeved and mounted on the extended end of the sleeve shaft.
[0015] Preferably, the interiors of the standby cavity and the culture cavity are both provided with temperature regulating holes, and the top ends of the inner walls of the standby cavity and the culture cavity away from the temperature regulating holes are both equipped with temperature measuring components.
[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. This strain concentration culture monitoring system uses the existing biological incubator to divide the biological incubator into a spare chamber and several culture chambers. A single culture chamber is a culture environment under a single temperature environment. Based on the conditions or characteristics of the liquid culture medium and the required cultured bacterial species, the culture vessel is sealed, air is supplied separately, and the culture bottle is stirred to ensure that the strains are evenly distributed in the culture liquid, which is conducive to uniform nutrition and facilitates the accuracy of subsequent sampling and even strain concentration detection results. It is also conducive to the one-to-one marking of culture bottles and unit capsules to be received samples in large quantities. While extracting samples with the help of air pressure and even capillary principles, it is convenient to collect multiple samples at one time, which facilitates the subsequent manual strain concentration detection operation of the samples.
[0017] 2. This strain concentration culture monitoring system can produce up and down and even left and right movement effects through the coordinated use of the first electric push rod and the second electric push rod. The up and down movement drives the sealing cover to move, which is convenient for the unified operation of opening and closing all culture bottles in a single culture chamber. The first electric push rod can drive the rack to move left and right, and then drive the transmission shaft to move by means of engagement, so as to rotate the stirring rod below, and then realize the stirring operation of the liquid culture medium inside a single group of multiple culture bottles with the help of a single first electric push rod to meet the culture needs.
[0018] 3. This strain concentration culture monitoring system uses a three-way solenoid valve to achieve three-way conduction and closing effects. While facilitating gas delivery, it can use the air pressure to make the liquid culture fluid flow into the temporary storage cylinder when the air pressure inside the culture bottle is high, and then be transported through the capillary tube to achieve small-dose sampling operations and reduce or avoid strain contamination of the trachea.
[0019] 4. This strain concentration culture monitoring system transports liquid culture medium into the first-class unit capsule or the second-class unit capsule through a capillary tube. When the first-class unit capsule or the second-class unit capsule is numbered, a directional and precise one-to-one transport effect can be achieved. When the sampling rack is removed, all the first-class unit capsules or the second-class unit capsules can be removed at the same time, so that samples of multiple sampling operations can be taken out at the same time. To a certain extent, when the culture bottle needs to be sealed, manual sampling one by one can be avoided after opening the culture bottle. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 This is a schematic diagram of the overall appearance structure of the present invention; Figure 2 Schematic diagram of the internal structure of the present invention; Figure 3 For the present invention Figure 2 A partial enlarged schematic diagram in the middle; Figure 4 This is a schematic diagram of the top view of the sampling rack of the present invention; Figure 5 Schematic diagram of the front structure of the limiting seat, the first type of unit capsule and the second type of unit capsule of the present invention; Figure 6 This is a schematic diagram of the combined appearance structure of the sealing cap and the culture bottle of the present invention; Figure 7 This is a schematic diagram of the internal structure of the combination of the sealing cover and the culture bottle of the present invention; Figure 8 For the present invention Figure 2 A partial enlarged schematic diagram of point B in the middle.
[0021] In the figure: 1. Biological incubator; 2. Split observation door; 3. Signal transceiver; 4. Control panel; 5. Sealed door; 6. Spare chamber; 7. Culture bottle; 8. Extension box; 9. Temperature adjustment hole; 10. Lifting frame; 11. Receiver tray; 12. Capillary tube; 13. Air pipe; 14. Culture chamber; 15. Air supply partition; 16. Temperature measuring component; 17. Synchronous slide; 18. Partition plate; 19. Guide frame; 20. Sampling rack; 21. Class I unit capsule; 22. Limit seat; 23. Injection port; 24. Class II unit capsule; 25. Sealing cover; 26. Three-way solenoid valve; 27. Electromagnetic flow sensor; 28. Drive shaft; 29. Gear; 30. Sleeve shaft; 31. Seal; 32. Temporary storage cylinder; 33. Multi-purpose tube; 34. Stirring rod; 35. First electric push rod; 36. Second electric push rod; 37. Rack. DETAILED DESCRIPTION
[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0023] In the description of the present invention, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," "the other end," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0024] like Figures 1 to 8As shown, the strain concentration culture monitoring system of this embodiment includes a biological incubator 1. The biological incubator 1 is an existing incubator with a separate gas supply (controlling the concentration of carbon dioxide, oxygen, nitrogen, etc.) and a temperature control function. The interior can be disinfected when not in use. The interior of the biological incubator 1 is provided with a chamber for strain culture space, and a sealed door 5 is installed on the front side of the chamber. A control panel 4 is provided on one side of the biological incubator 1. For easy operation, a touch screen can be used as the control panel. A signal transceiver 3 is provided on the top of the biological incubator 1 to receive external signals and feedback signals. If sampling is required, a sampling signal can be sent by the remote control terminal. The signal is received by the signal transceiver 3 and processed by the internal circuit. A partition plate 18 is provided at the bottom of the chamber of the biological incubator 1, and the partition plate 18 divides the chamber from top to bottom into a culture chamber and a spare chamber 6. The spare chamber 6 can store some items or a blank control group of culture medium. A plurality of air supply partitions 15 are provided from top to bottom inside the culture chamber. The air supply partition 15 is hollow inside and can be connected to the air supply system inside the biological incubator 1 through a pipeline to provide other sources such as carbon dioxide. The air supply partition 15 divides the culture chamber into a plurality of culture chambers 14. Different culture chambers 14 can be set to different temperatures, thereby selecting multiple or single strains to be cultured at different temperatures at the same time; The two side walls of the culture chamber 14 are both equipped with guide racks 19, which are similar to conventional support racks. The left and right guide racks 19 are a group, and the opposite side walls are both provided with slide assemblies. The side walls of the left and right guide racks 19 are slidably connected with a receiving tray 11. The two guide racks 19 support a single receiving tray 11 at the same time, and the receiving tray 11 can be loaded and unloaded along the slide. The surface of the receiving tray 11 is limited by a number of through holes to be installed with a culture bottle 7. The culture bottle 7 is limited in the through holes of the receiving tray 11, and the liquid culture medium is contained inside, which is an environment for the growth of the strain. The biological incubator 1 on one side of the culture chamber 14 is internally installed with an extension box 8, which protrudes from the entire biological incubator 1 and is sealed. The interior of the extension box 8 is equipped with a lifting frame 10 through a power mechanism, and a plurality of transmission shafts 28 are installed at the bottom of the lifting frame 10 through bearings. The power mechanism can drive the lifting frame 10 to move up and down, and can drive the transmission shaft 28 to rotate. The bottom of the transmission shaft 28 is detachably equipped with a sealing cover 25, which is used in conjunction with the culture bottle 7. The sealing cover 25 moves up and down with the lifting frame 10 and seals the culture bottle 7. The transmission shaft 28 can provide the required power for stirring the liquid culture medium inside the culture bottle 7. A three-way solenoid valve 26 is installed on the top of the sealing cover 25, which itself has a three-way conduction effect, and one end of the three-way solenoid valve 26 extends to the bottom of the sealing cover 25;Several air tubes 13 are installed at the top of the inner walls of several culture chambers 14. Except for the topmost culture chamber 14, the top of the inner walls of all other culture chambers 14 forms the bottom of the air supply partition 15. Therefore, except for the topmost culture chamber 14, the heating tubes 13 must extend through the air supply partition 15 to the air supply system at the rear of the biological incubator 1. A sampling rack 20 is slidably connected to the tops of the left and right guide frames 19. A sampling mechanism is connected between the surface of the sampling rack 20 and the three-way solenoid valve 26. The sampling rack 20 receives samples collected by the sampling mechanism in the culture chamber 14. Simply manually remove the sampling rack 20 as a whole to remove a large number of samples for direct transportation.
[0025] Specifically, the surface of the sealed door 5 on the front side of the culture chamber 14 is equipped with a split observation door 2. Different from the sealed door 5, the split observation door 2 can be opened to open the corresponding culture chamber 14 and perform operations in a single culture chamber 14.
[0026] Furthermore, a synchronous slide 17 is installed inside the biological incubator 1 on one side of the culture chamber 14. The synchronous slide 17 extends up and down, and the synchronous slide 17 and the extension box 8 are distributed relatively to each other on the left and right. The end of the lifting frame 10 is connected to the synchronous slide 17 through a slider. When the lifting frame 10 moves up and down, the synchronous slide 17 can guide one end of the lifting frame 10 to move.
[0027] Furthermore, the power mechanism includes a first electric push rod 35, a second electric push rod 36, a rack 37 and a gear 29. The second electric push rod 36 is installed at the bottom end of the inner wall of the extension box 8. The output rod of the second electric push rod 36 extends vertically upward, which can generate power for upward movement. The end of the output rod of the second electric push rod 36 is installed with the first electric push rod 35. The output rod of the first electric push rod 35 extends toward the synchronous slide groove 17, which can generate horizontal movement toward the synchronous slide groove 17. The end of the output rod of the first electric push rod 35 is installed with a rack 37, and a tooth groove is provided on the inner side of the rack 37. The surface of the transmission shaft 28 is installed with a gear 29, which meshes with the rack 37, and the top of the shell of the first electric push rod 35 is connected to the lifting frame 10. When the first electric push rod 35 is extended and retracted, it can drive the rack 37 to move left and right, and then under the action of the gear teeth meshing, the transmission shaft 28 is rotated, and the second electric push rod 36 can drive the lifting frame 10 to move up and down, thereby driving the sealing cover 25 to move up and down.
[0028] Furthermore, the three-way solenoid valve 26 is provided with two output ends and one input end, the input end is connected to the trachea 13, and the two need to be connected by a snap-on connection for easy disassembly. The three-way solenoid valve 26 controls the switch operation of the trachea 13, and one output end extends to the bottom of the sealing cover 25 and is connected to a multi-purpose tube 33. The power supply component of the three-way solenoid valve 26 itself is a plug-in connection form, and the plug-in port needs to be waterproofed to facilitate subsequent disinfection and even reuse. In actual use, the multi-purpose tube 33 extends into the culture bottle 7, which is similar to an aeration tube and can be used for aeration in the liquid culture medium to transport the gas required for the culture strain, and the other output end is installed with a temporary storage tube 32, which is a transparent columnar structure, and the inner diameter gradually decreases in the direction away from the three-way solenoid valve 26.
[0029] Furthermore, the sampling mechanism includes a unit component, a capillary 12 and an electromagnetic flow sensor 27. The electromagnetic flow sensor 27 is installed on the temporary storage tube 32 and can detect the flow situation inside the temporary storage tube 32. The electromagnetic flow sensor 27 feeds back the signal to the controller, and the discharge port of the temporary storage tube 32 is plugged and installed with a capillary 12. The inner diameter of the capillary 12 is very small, and the liquid sample is transported relatively slowly. A unit component is installed between the capillary 12 and the sampling rack 20 for storing the sample after sampling. There are as many sampling mechanisms as there are culture bottles 7.
[0030] Furthermore, the unit assembly includes a type of unit capsule 21 and a limiting seat 22. Several limiting seats 22 are provided on the surface of the sampling rack 20. The limiting seats 22 can be arranged individually or in multiple arrangements. The top and one side of the limiting seat 22 are both opened, and the side opening is similar to a slide groove. Its size decreases from the outside to the inside. The type of unit capsule 21 is installed on the limiting seat 22 through the side opening. The type of unit capsule 21 is a plastic product. A slider-like structure is provided on both sides of the bottom of the unit capsule, which can be installed with an interference fit after sliding movement with the side opening. The side surface of the type of unit capsule 21 is provided with an injection port 23. The injection port 23 is detachably connected to the capillary 12, and the two can be connected by plugging. The liquid culture medium transported by the capillary 12 is injected into the type of unit capsule 21 through the injection port 23.
[0031] Furthermore, several limiting seats 22 are arranged adjacent to each other, and several adjacent limiting seats 22 are equipped with second-class unit capsules 24. The second-class unit capsules 24 are composed of several first-class unit capsules 21 interconnected through pipes, that is, the second-class unit capsules 24 are similar to a combination of multiple first-class unit capsules 21. The internal volume of the first-class unit capsules 21 can be used as the sample capacity required for a single test. When multiple tests are required, the second-class unit capsules 24 can be used to sample the liquid culture medium.
[0032] Furthermore, a sealing member 31 is provided in the inner cavity at the bottom of the sealing cover 25, and a rubber gasket can be used as a sealing material to ensure the sealing effect of the culture bottle 7. The bottom end of the transmission shaft 28 is detachably mounted with a sleeve shaft 30, and a through hole is provided on the top of the sleeve shaft 30. After the bottom of the transmission shaft 28 extends into the interior of the sleeve shaft 30, it is limitedly installed with the help of a screw hole screw, which is convenient for disassembly. A screw head can be provided at the end of the screw to facilitate manual rotation and removal of the screw. The bottom end of the sleeve shaft 30 extends to the bottom of the sealing cover 25, and the two are connected by bearings, and a stirring rod 34 is sleeved on the extended end of the sleeve shaft 30. The rotation of the transmission shaft 28 can drive the stirring rod 34 to rotate synchronously, thereby stirring the liquid culture medium, which can ensure the uniformity of the strain culture and facilitate the accuracy of sampling.
[0033] Furthermore, the interior of the standby chamber 6 and the culture chamber 14 are both provided with temperature adjustment holes 9, which can discharge cold air, which is conducive to cooling operations inside the standby chamber 6 and the culture chamber 14. The top of the inner wall of the standby chamber 6 and the culture chamber 14 away from the temperature adjustment holes 9 are both installed with temperature measuring components 16, which are temperature sensors that can monitor the temperature inside the standby chamber 6 and the culture chamber 14.
[0034] The method of use of this embodiment is as follows: To meet the needs of strain culture, if a large number of organisms need to be cultured, the front-to-back depth of the entire biological incubator 1 is larger, and other configurations may also need to be modified. For example, two sets of front and back through-holes can be provided on the receiving tray 11 to facilitate the storage of two sets of culture bottles 7. Two front and back lifting racks 10 or even two sets of power mechanisms can be configured. The number of loops of air supply lines and air pipes 13 required will depend on the number of culture bottles 7 that can be stored. Before actual use, the entire biological incubator 1 must be disinfected. According to the number and type of culture required, several sterilized sealing caps 25 are manually installed on the lower part of the drive shaft 28, and the input end of the three-way solenoid valve 26 is connected to the corresponding air pipe 13. The culture bottles 7 containing liquid culture medium after inoculation are placed in a single receiving tray 11 along with a blank control group. After placing the receiving tray 11 containing the culture bottles 7 on the guide frame 19, the second electric push rod 36 is activated to retract, causing the lifting frame 10 and even the sealing cover 25 to move downward. The sealing cover 25 seals the culture bottles 7 directly below. At this point, the sampling rack 20, equipped with the first-class unit capsules 21 and / or second-class unit capsules 24, is manually installed on top of the guide frame 19. Each first-class unit capsule 21 or second-class unit capsule 24 is numbered. The guide frame 19 is located between the drive shafts 28. Different-numbered unit capsules are connected to different temporary storage cylinders 32 via capillaries 12. Different numbers correspond to different strains in different cultures, forming a corresponding relationship. The numbers and corresponding relationship are input into the controller via the control panel 4, facilitating digital control and direct, targeted concentration measurement after subsequent sampling. During the subsequent culture process, the first electric push rod 35 can be activated to reciprocate and extend, thereby driving the rack 37 to move left and right. The drive shaft 28 drives the stirring rod 34 to rotate, ensuring uniform strain distribution and nutrient distribution within the liquid culture medium. The internal pressure of the culture chamber 14 is close to atmospheric pressure, but is affected by factors such as the gas supply from the trachea 13 in the culture bottle 7 and the gas generated by the growth of bacteria, so the internal pressure of the culture bottle 7 is greater than that of the culture chamber 14 .If it is necessary to take samples to detect the concentration of bacterial culture after a growth period, there is no need to open the sealing cover 25 to avoid the influence of external bacteria. The three-way electromagnetic valve 26 is directly controlled to close the air pipe 13 and open the channel of the temporary storage cylinder 32. Under the influence of air pressure, the culture liquid carrying the strain will flow upward through the multi-purpose tube 33. After passing through the temporary storage cylinder 32 and the capillary tube 12, the culture liquid flows into the first unit capsule 21 and or the second unit capsule 24. The electromagnetic flow sensor 27 detects the flow change. When it reaches a certain value, , a signal can be fed back to the three-way solenoid valve 26, which instantly closes the multi-purpose tube 33 and opens the air pipe 13 for air supply. Under the influence of air pressure, the residual liquid inside the three-way solenoid valve 26 continues to flow through the temporary storage cylinder 32 to the capillary tube 12 and even the unit capsule, reducing or even preventing the growth of residual strains within the three-way solenoid valve 26. After a certain period of operation, the temporary storage cylinder 32 is closed. During the subsequent strain culture process, the air pipe 13 continues to supply air to the culture bottle 7, preventing residual liquid from remaining in the multi-purpose tube 33. During manual sampling, one only needs to open the split observation door 2 or the entire sealing door 5, remove the capillary tube 12 from the temporary storage cylinder 32, and remove the sampling rack 20 from the guide rack 19. All samples in a single culture chamber 14 can be directly removed. Subsequently, samples are removed from the interior of each type I unit capsule 21 or type II unit capsule 24 one by one using a sampler and concentration measured, thereby obtaining the strain concentration inside the corresponding culture bottle 7.
[0035] After completing a culture, the sleeve shaft 30 and even the entire sealing cover 25 underneath it can be manually removed by screwing the head, and the power supply of the three-way solenoid valve 26 can be unplugged. The culture fluid has never entered the trachea 13. It is only necessary to clean and disinfect the trachea 13 regularly. The sealing cover 25 and even the parts above it can be reused after being disinfected as a whole. The three-way solenoid valve 26 and the electromagnetic flow sensor 27 need to be waterproofed. The capillary 12, the first-class unit capsule 21 or the second-class unit capsule 24 are plastic products and can be used as disposable sampling.
[0036] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalent features for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.
Claims
1. A strain concentration culture monitoring system, comprising a biological incubator (1), wherein a chamber is provided inside the biological incubator (1), and a sealing door (5) is installed on the front side of the chamber, a control panel (4) is provided on one side of the biological incubator (1), and a signal transceiver (3) is provided on the top of the biological incubator (1), characterized in that: The biological incubator (1) has a chamber bottom provided with a partition plate (18), which divides the chamber from top to bottom into a culture chamber and a standby chamber (6); the culture chamber has a plurality of air supply partition plates (15) provided from top to bottom, which divide the culture chamber into a plurality of culture chambers (14); Guide racks (19) are installed on both side walls of the culture chambers (14), and a receiving plate (11) is slidably connected between the side walls of the left and right guide racks (19). Culture bottles (7) are installed on the surface of the receiving plate (11) through a plurality of through-holes. Extension boxes (8) are installed inside the biological culture boxes (1) on one side of the culture chamber (14). A lifting rack (10) is installed inside the extension box (8) via a power mechanism. A plurality of transmission shafts (28) are installed at the bottom of the lifting rack (10) via bearings. A sealing cover (25) is detachably installed at the bottom of the transmission shaft (28). The sealing cover (25) is used in conjunction with the culture bottle (7). A three-way solenoid valve (26) is installed on the top of the sealing cover (25), and one end of the three-way solenoid valve (26) extends to the bottom of the sealing cover (25). A plurality of air tubes (13) are installed on the top of the inner wall of the plurality of culture chambers (14), and a sampling rack (20) is slidably connected to the top of the left and right guide racks (19), and a sampling mechanism is connected between the surface of the sampling rack (20) and the three-way solenoid valve (26).
2. The bacterial strain concentration culture monitoring system according to claim 1, characterized in that: The surface of the sealed door (5) on the front side of the culture chamber (14) is equipped with a split observation door (2).
3. The bacterial strain concentration culture monitoring system according to claim 1, characterized in that: A synchronous slide (17) is installed inside the biological culture box (1) on one side of the culture chamber (14). The synchronous slide (17) and the extension box (8) are arranged in a left-right relative manner, and the end of the lifting frame (10) is connected to the synchronous slide (17) through a slider.
4. The bacterial strain concentration culture monitoring system according to claim 3, characterized in that: The power mechanism includes a first electric push rod (35), a second electric push rod (36), a rack (37) and a gear (29), the second electric push rod (36) is installed at the bottom end of the inner wall of the extension box (8), the output rod of the second electric push rod (36) extends vertically upward, the end of the output rod of the second electric push rod (36) is installed with the first electric push rod (35), the output rod of the first electric push rod (35) extends toward the synchronous slide groove (17), the end of the output rod of the first electric push rod (35) is installed with a rack (37), the surface of the transmission shaft (28) is installed with a gear (29), the gear (29) is meshed with the rack (37), and the top of the shell of the first electric push rod (35) is connected to the lifting frame (10).
5. The bacterial strain concentration culture monitoring system according to claim 1, characterized in that: The three-way solenoid valve (26) is provided with two output ends and one input end, the input end is connected to the air pipe (13), one output end extends to the bottom of the sealing cover (25) and is connected to the multi-purpose tube (33), and the other output end is installed with a temporary storage cylinder (32).
6. The bacterial strain concentration culture monitoring system according to claim 5, characterized in that: The sampling mechanism comprises a unit assembly, a capillary tube (12) and an electromagnetic flow sensor (27); the electromagnetic flow sensor (27) is mounted on a temporary storage cylinder (32); the discharge port of the temporary storage cylinder (32) is plugged with the capillary tube (12); and the unit assembly is mounted between the capillary tube (12) and the sampling rack (20).
7. The bacterial strain concentration culture monitoring system according to claim 6, characterized in that: The unit assembly includes a type of unit capsule (21) and a limiting seat (22). A plurality of limiting seats (22) are provided on the surface of the sampling rack (20). The top and one side of the limiting seat (22) are both opened. The type of unit capsule (21) is installed on the limiting seat (22) through the side opening. The side surface of the type of unit capsule (21) is provided with an injection port (23), and the injection port (23) is detachably connected to the capillary (12).
8. The bacterial strain concentration culture monitoring system according to claim 7, characterized in that: A plurality of the limiting seats (22) are arranged adjacent to each other, and a second-type unit capsule (24) is installed on a plurality of adjacent limiting seats (22). The second-type unit capsule (24) is composed of a plurality of first-type unit capsules (21) interconnected via a pipeline.
9. The bacterial strain concentration culture monitoring system according to claim 1, characterized in that: The bottom inner cavity of the sealing cover (25) is provided with a sealing member (31), and the bottom end of the transmission shaft (28) is detachably mounted with a sleeve shaft (30), the bottom end of the sleeve shaft (30) extends below the sealing cover (25), and the extended end of the sleeve shaft (30) is sleeved with a stirring rod (34).
10. The bacterial strain concentration culture monitoring system according to claim 1, characterized in that: The standby chamber (6) and the culture chamber (14) are both provided with a temperature adjustment hole (9) inside, and a temperature measuring component (16) is installed on the top of the inner wall of the standby chamber (6) and the culture chamber (14) away from the temperature adjustment hole (9).