Liver-protecting traditional Chinese medicine compound health-care vinegar as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of health-care food, and particularly relates to liver-protecting traditional Chinese medicine compound health-care vinegar as well as a preparation method and application thereof. Raw materials of the liver-protecting traditional Chinese medicine composite health-care vinegar comprise traditional Chinese medicine water extract and table vinegar, and traditional Chinese medicine raw materials for preparing the traditional Chinese medicine water extract comprise radix puerariae, hovenia dulcis thunb, radix paeoniae alba and schisandra chinensis. Experiments show that the liver-protecting traditional Chinese medicine composite health-care vinegar has a good liver-protecting function, can reduce fat deposition in the liver, relieve liver inflammation damage and improve the liver antioxidant level, and is beneficial to maintaining liver health. The preparation method is simple and easy to implement, short in period, good in stability and suitable for large-scale production.

Description

Technical Field

[0001] The invention belongs to the technical field of health-care foods, and in particular relates to a liver-protecting traditional Chinese medicine composite health-care vinegar, a preparation method and an application thereof. Background Art

[0002] The liver is the largest organ in the human body, responsible for numerous critical physiological functions, including metabolism, detoxification, bile synthesis and secretion, immunity, and blood coagulation. However, in modern society, the incidence of liver disease is increasing year by year due to factors such as environmental pollution, poor dietary habits, excessive alcohol consumption, and long-term drug use. Alcohol is a major cause of liver damage, typically caused by excessive or long-term drinking. During liver metabolism, alcohol can cause varying degrees of liver damage, the earliest and most common being fatty liver, in which abnormal lipid metabolism leads to fat deposition within liver cells, resulting in fatty degeneration. During alcohol metabolism, alcohol also produces excessive ROS, which in turn causes oxidative stress. At the same time, alcohol and its metabolites can trigger an inflammatory response, further exacerbating liver damage.

[0003] Common liver problems include fatty liver disease, hepatitis, cirrhosis, and liver cancer. These problems not only severely impact patients' quality of life but can also lead to serious complications or even be life-threatening. Preventing or alleviating liver damage is an effective strategy to avoid these liver problems.

[0004] There are many types of liver protection products on the market. Many products contain chemical additives, and some products also contain irritating ingredients. Long-term use may cause side effects and irritation, and may even cause adverse reactions. However, liver protection products that do not contain chemical additives do not all produce ideal liver protection effects. Summary of the Invention

[0005] To address the above technical issues, the present invention provides a liver-protecting traditional Chinese medicine compound health-care vinegar, its preparation method, and its use. The liver-protecting traditional Chinese medicine compound health-care vinegar provided by the present invention contains no chemical additives, is safe and mild, and can effectively inhibit liver damage. It can be used to prepare liver-protecting products, particularly those that prevent or alleviate acute liver damage caused by alcohol.

[0006] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions: A first aspect of the present invention provides a liver-protecting traditional Chinese medicine composite health-care vinegar. The raw materials thereof comprise, by weight, 28 to 32 parts of a traditional Chinese medicine aqueous extract and 13 to 40 parts of edible vinegar. The traditional Chinese medicine raw materials for preparing the traditional Chinese medicine aqueous extract comprise kudzu root, hovenia dulcis fruit, white peony root and schisandra chinensis, and the mass ratio of the kudzu root, hovenia dulcis fruit, white peony root and schisandra chinensis is (1.7 to 2.3): (0.8 to 1.2): (1.7 to 2.3): (0.8 to 1.2).

[0007] Each Chinese medicine in the present invention has the following effects: Pueraria root: Sweet and pungent in flavor, with a cooling nature, it enters the spleen, stomach, and lung meridians. It relieves muscle tension, reduces fever, promotes fluid production, quenches thirst, clears rashes, promotes yang and stops diarrhea, promotes blood circulation, and detoxifies alcohol. Modern pharmacological research indicates that Pueraria root contains flavonoids, terpenes, steroids, coumarins, organic acids, alkaloids, and other bioactive ingredients. It has multiple pharmacological functions, including accelerating alcohol metabolism, providing antioxidant effects, protecting the liver and myocardial cells, and providing neuroprotection.

[0008] Hovenia dulcis (Curcuma longituba) fruit: Sweet in flavor and neutral in nature, it enters the Heart, Liver, and Lung meridians. It detoxifies alcohol, quenches thirst, relieves restlessness, stops vomiting, and promotes urination. Modern pharmacological research indicates that Hovenia dulcis (Curcuma longituba) fruit contains bioactive ingredients such as fatty acids, flavonoids, terpenes, phenylpropanoids, and alkaloids. It exhibits a variety of pharmacological benefits, including alcohol detoxification, liver protection, fatigue relief, lipid peroxidation resistance, anti-tumor properties, overall physical fitness enhancement, and neuroprotection.

[0009] White peony root (Radix Paeoniae Alba) is bitter and sour in flavor, slightly cold in nature, and enters the liver and spleen meridians. It nourishes blood and regulates menstruation, astringes yin and stops sweating, softens the liver and relieves pain, and calms liver yang. Modern pharmacological research has shown that white peony root contains bioactive ingredients such as monoterpenes, glycosides, triterpenes, tannins, flavonoids, volatile oils, phenylethanoid glycosides, and steroids. It exhibits multiple pharmacological activities, including analgesia, anti-inflammatory, antioxidant activity, anti-hepatic fibrosis, and hepatoprotection.

[0010] Schisandra chinensis: Sour and sweet, warm in nature, it enters the lung, heart, and kidney meridians. It has astringent and firming properties, replenishes Qi and promotes fluid production, nourishes the kidneys and calms the mind, and detoxifies alcohol. Modern pharmacological research has shown that Schisandra chinensis contains bioactive ingredients such as lignans, polysaccharides, terpenes, organic acids, and volatile oils, exhibiting multiple pharmacological functions, including antioxidant activity, liver protection, treatment of liver damage, anti-aging, and immune enhancement.

[0011] Among them, Pueraria lobata and Hovenia dulcis are traditional Chinese medicines listed in the "List of Items that are Both Food and Medicine", while White Peony Root and Schisandra chinensis are traditional Chinese medicines listed in the "List of Items that Can Be Used as Health Foods", and both have high safety.

[0012] Vinegar is a common condiment. From a Traditional Chinese Medicine (TCM) perspective, sourness enters the liver, and vinegar can guide medicinal herbs into the liver, thus possessing numerous health benefits. Modern research further reveals that vinegar contains bioactive substances such as melanoidins, flavonoids, and polyphenols, which, by scavenging oxygen free radicals and reducing oxidative stress, exert lipid-lowering, antioxidant, cardiovascular disease prevention, and anti-tumor effects.

[0013] The liver-protecting traditional Chinese medicine composite health vinegar provided by the present invention combines edible vinegar with traditional Chinese medicines that are both edible and medicinal, as well as traditional Chinese medicines that can be used in health foods. It can produce significant liver-protecting effects without adding any chemical additives. It can effectively prevent and alleviate liver damage, provide comprehensive protection for the liver, and take into account the safety and mildness of the product.

[0014] Research has confirmed that various Chinese medicinal herbs with edible and medicinal properties have hepatoprotective effects. Experimental studies using Pueraria lobata, Hovenia dulcis fruit, White Peony Root, and Schisandra chinensis as the raw materials for the aqueous extract of this invention have shown that these four herbs exhibit significant synergistic effects in protecting the liver against liver damage, particularly acute alcohol-induced liver damage. This synergistic effect can achieve more ideal effects, including promoting liver fat metabolism, improving liver function, and providing antioxidant benefits.

[0015] Preferably, the mass ratio of the traditional Chinese medicine aqueous extract to edible vinegar is 30:13.

[0016] Preferably, the mass ratio of Pueraria lobata, Hovenia dulcis, White Peony Root and Schisandra chinensis is 2:1:2:1.

[0017] Preferably, the edible vinegar is Shanxi aged vinegar with an acidity between 7 and 8.

[0018] Preferably, the raw materials of the liver-protecting traditional Chinese medicine composite health vinegar also include 7 to 20 parts of purified water.

[0019] Preferably, the raw materials of the liver-protecting traditional Chinese medicine composite health-care vinegar also include flavoring agents.

[0020] Optionally, the flavoring agent is selected from one or more of honey, sucrose, steviol glycosides, mogrosides, disodium glycyrrhizate, xylitol, erythritol, and sorbitol.

[0021] Preferably, the preparation method of the Chinese herbal medicine aqueous extract comprises: ultrasonically extracting the Chinese herbal medicine raw material 2-3 times with purified water at 90-110°C, with the amount of purified water used each time being 25-30 times the mass of the Chinese herbal medicine raw material, and each ultrasonic extraction lasting 90-105 minutes. The extracts obtained from each extraction are combined and concentrated to 1 / 35-1 / 45 of the original total volume to obtain the Chinese herbal medicine aqueous extract. By adopting the above-mentioned process parameters, this preparation method can retain the active ingredients to a large extent, thereby ensuring the liver-protecting effect of the product.

[0022] Further preferably, the temperature of the ultrasonic extraction is 100°C.

[0023] Further preferably, the ultrasonic extraction is performed twice, each time for 90 minutes.

[0024] More preferably, the ultrasonic extraction is performed at an ultrasonic frequency of 2 to 4 kHz. Even more preferably, the ultrasonic extraction is performed at an ultrasonic frequency of 3 kHz.

[0025] More preferably, the concentration temperature is 60-70° C. Optionally, the concentration method is vacuum concentration at a pressure of -0.08 to -0.10 MPa.

[0026] More preferably, the volume is concentrated to 1 / 40 of the original total volume.

[0027] The second aspect of the present invention provides a method for preparing the above-mentioned liver-protecting traditional Chinese medicine composite health-care vinegar, which specifically comprises the following steps: S1, mixing the raw materials by weight, removing the solid precipitate, and collecting the clear liquid; S2. Bottle and sterilize the clear liquid to obtain the liver-protecting Chinese medicinal compound health-care vinegar.

[0028] Preferably, the solid precipitate is removed in S1 by centrifugation or filtration, and the supernatant is collected after centrifugation or the filtrate is collected after filtration.

[0029] Further preferably, the centrifugation method is: centrifuging at 1000-1500 r / min for 10-15 min and collecting the supernatant.

[0030] Preferably, the sterilization method in S2 is ultra-high temperature instantaneous sterilization at 125°C for 3 seconds.

[0031] The third aspect of the present invention provides the use of the above-mentioned liver-protecting traditional Chinese medicine composite health-care vinegar in the preparation of liver-protecting products.

[0032] Preferably, the liver protection product is a product for preventing or alleviating acute liver damage caused by alcohol.

[0033] Preferably, the liver protection product is a health food that has an auxiliary protective effect on chemical liver damage.

[0034] The beneficial effects of the present invention are: 1. The liver-protecting Chinese medicine composite health-care vinegar provided by the present invention does not contain chemical additives. The product is safe and mild, suitable for daily or long-term drinking. It can be consumed directly or blended according to personal taste, thereby increasing user acceptance and satisfaction, and providing consumers with a new choice of daily health-care beverage that is convenient, delicious, safe, and effective. 2. The present invention has been strictly selected and scientifically proportioned, and uses specific proportions of Pueraria lobata, Hovenia dulcis, White Peony Root and Schisandra chinensis as the Chinese medicinal raw materials for the Chinese medicinal water extract to ensure that the product has an ideal liver protection function. The present invention combines the above four Chinese medicinal materials with liver protection effects with edible vinegar, and achieves a synergistic effect through the compounding of Chinese medicinal materials and the compounding of Chinese medicinal materials with edible vinegar, so that the product can produce a significant liver protection effect. Experiments have shown that for acute liver damage caused by alcohol, the liver-protecting Chinese medicinal compound health vinegar provided by the present invention can effectively reduce fatty degeneration of liver cells, reduce fat deposition in the liver, alleviate liver inflammatory damage, and improve the liver's antioxidant function; 3. The preparation method provided by the present invention has simple process steps and is easy to operate, making it suitable for large-scale production. The efficient and easy-to-implement process ensures high product quality and significantly improves production feasibility and efficiency, effectively meeting market demand. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the preparation process of the liver-protecting traditional Chinese medicine composite health-care vinegar of the present invention; Figure 2 This is the Oil Red O staining result of the liver of each group of mice in Test Example 1 of the present invention; Figure 3 The graph is the test result of biochemical indexes of serum and liver of each group of mice in Test Example 1 of the present invention; compared with the model control group, * P<0.05, ** P < 0.01, *** P < 0.001; Figure 4 This is the observation result of storage stability of liver-protecting Chinese medicinal compound health-care vinegar in Test Example 2 of the present invention; Figure 5 This is the stability test result of the total acid content of the liver-protecting traditional Chinese medicine composite health-care vinegar in Test Example 2 of the present invention; different capital letters indicate that different samples were compared on the same day, P < 0.05; different lowercase letters indicate that the same sample was compared on different days, P < 0.05; Figure 6 This is the test result of the antioxidant DPPH activity stability of the liver-protecting traditional Chinese medicine compound health-care vinegar in Test Example 2 of the present invention. Different capital letters indicate that P < 0.05 was found when different samples were compared on the same day; different lowercase letters indicate that P < 0.05 was found when the same sample was compared on different days. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0037] Liver damage can lead to a variety of liver problems, including fatty liver, hepatitis, cirrhosis, and liver cancer. These problems not only reduce patients' quality of life but can also lead to serious complications and even be life-threatening. Currently, liver protection products on the market contain chemical additives or irritating ingredients, resulting in suboptimal liver protection.

[0038] The present invention provides a liver-protecting traditional Chinese medicine composite health-care vinegar. The raw materials, by weight, include: 28-32 parts of a traditional Chinese medicine aqueous extract and 13-40 parts of edible vinegar. The traditional Chinese medicine raw materials used to prepare the traditional Chinese medicine aqueous extract include kudzu root, hovenia dulcis fruit, white peony root, and schisandra chinensis, with the mass ratio of kudzu root, hovenia dulcis fruit, white peony root, and schisandra chinensis being (1.7-2.3): (0.8-1.2): (1.7-2.3): (0.8-1.2). This liver-protecting traditional Chinese medicine composite health-care vinegar combines edible vinegar with traditional Chinese medicinal materials that are both medicinal and edible, as well as traditional Chinese medicinal materials that can be used in health foods, and can produce significant liver-protecting effects without the addition of any chemical additives.

[0039] The present invention also provides a method for preparing the liver-protecting Chinese medicine composite health-care vinegar, which specifically comprises the following steps: S1, mixing the raw materials by weight, removing the solid precipitate, and collecting the clear liquid; S2. Bottle and sterilize the clear liquid to obtain the liver-protecting Chinese medicinal compound health-care vinegar.

[0040] The embodiment of the present invention also provides the use of the above-mentioned liver-protecting traditional Chinese medicine composite health-care vinegar in the preparation of liver-protecting products.

[0041] The solutions of the present invention are described below through specific embodiments.

[0042] The Chinese medicinal materials used in the following examples all comply with the implementation standards of the 2020 edition of the Pharmacopoeia of the People's Republic of China.

[0043] The edible vinegar used in the following examples is Shanxi aged vinegar provided by Shanxi Zilin Vinegar Industry Co., Ltd., the product implementation standard is: GB / T 19777, and the total acid content is 7.5 g / 100 mL.

[0044] Unless otherwise specified, the raw materials, reagents, drugs, or instruments used in the following examples are all commercially available products. The methods used in the following examples are all conventional methods in the art unless otherwise specified.

[0045] Example 1 This embodiment provides a liver-protecting traditional Chinese medicine composite health-care vinegar, which is prepared from the following raw materials in parts by weight: 30 parts of traditional Chinese medicine aqueous extract, 13 parts of edible vinegar, and 7 parts of purified water.

[0046] The preparation method of the liver-protecting traditional Chinese medicine composite health-care vinegar is as follows: (1) Weigh 1000 g of Pueraria root, 500 g of Hovenia dulcis fruit, 1000 g of White Peony Root, and 500 g of Schisandra chinensis, wash them separately, mix them, add 75 kg of purified water, and perform ultrasonic extraction at 100°C for 1.5 hours at an ultrasonic frequency of 3 kHz. After the extraction, filter out the residue and collect the filtrate. Then, add 75 kg of purified water to the residue again, perform ultrasonic extraction at 100°C for 1.5 hours at an ultrasonic frequency of 3 kHz. After the extraction, filter out the residue and collect the filtrate. Combine the filtrates obtained from the two times and vacuum concentrate them at a pressure of -0.08 to -0.10 MPa and a temperature of 60-70°C to 1 / 40 of the original total volume to obtain a Chinese medicine aqueous extract.

[0047] (2) The prepared Chinese medicine water extract, edible vinegar and purified water were mixed according to the above weight proportions, and then centrifuged at 1500 r / min for 10 min, and the supernatant was collected.

[0048] (3) The prepared supernatant is bottled and sterilized, and then ultra-high temperature instantaneous sterilization is performed at 125°C for 3 seconds to obtain the liver-protecting traditional Chinese medicine composite health vinegar.

[0049] Preparation flow chart as shown Figure 1 shown.

[0050] Example 2 This embodiment provides a liver-protecting traditional Chinese medicine composite health-care vinegar, which is prepared from the following raw materials in parts by weight: 30 parts of traditional Chinese medicine aqueous extract, 27 parts of edible vinegar, and 13 parts of purified water.

[0051] The preparation method of the liver-protecting traditional Chinese medicine composite health-care vinegar is the same as that of Example 1.

[0052] Example 3 This embodiment provides a liver-protecting traditional Chinese medicine composite health-care vinegar, which is prepared from the following raw materials in parts by weight: 30 parts of traditional Chinese medicine aqueous extract, 40 parts of edible vinegar, and 20 parts of purified water.

[0053] The preparation method of the liver-protecting traditional Chinese medicine composite health-care vinegar is the same as that of Example 1.

[0054] Example 4 This embodiment provides a liver-protecting traditional Chinese medicine composite health-care vinegar, which is prepared from the following raw materials in parts by weight: 28 parts of traditional Chinese medicine aqueous extract, 13 parts of edible vinegar, 15 parts of purified water, and 4 parts of honey.

[0055] The preparation method of the liver-protecting traditional Chinese medicine composite health-care vinegar is as follows: (1) Weigh 170 g of Pueraria root, 120 g of Hovenia dulcis fruit, 170 g of White Peony Root and 120 g of Schisandra chinensis, wash them separately and mix them, add 17.4 kg of purified water, and perform ultrasonic extraction at 90 °C for 105 min at an ultrasonic frequency of 4 kHz. After the extraction, filter out the residue and collect the filtrate. Then, add 17.4 kg of purified water to the residue again, perform ultrasonic extraction at 90 °C for 90 min at an ultrasonic frequency of 4 kHz. After the extraction, filter out the residue and collect the filtrate. Add 17.4 kg of purified water to the residue again, perform ultrasonic extraction at 90 °C for 90 min at an ultrasonic frequency of 4 kHz. After the extraction, filter out the residue and collect the filtrate. Combine the filtrates obtained three times and vacuum concentrate them at a pressure of -0.08 to -0.10 MPa and a temperature of 60-70 °C to 1 / 45 of the original total volume to obtain a Chinese medicine aqueous extract.

[0056] (2) The prepared Chinese herbal medicine aqueous extract, edible vinegar and purified water were mixed according to the above weight proportions, and then centrifuged at 1000 r / min for 15 min, and the supernatant was collected.

[0057] (3) The prepared supernatant is bottled and sterilized, and then ultra-high temperature instantaneous sterilization is performed at 125°C for 3 seconds to obtain the liver-protecting traditional Chinese medicine composite health vinegar.

[0058] Example 5 This embodiment provides a liver-protecting traditional Chinese medicine composite health-care vinegar, which is prepared from the following raw materials in parts by weight: 32 parts of traditional Chinese medicine aqueous extract, 13 parts of edible vinegar, 22 parts of purified water, and 3 parts of xylitol.

[0059] The preparation method of the liver-protecting traditional Chinese medicine composite health-care vinegar is as follows: (1) Weigh 230 g of Pueraria root, 80 g of Hovenia dulcis fruit, 230 g of White Peony Root, and 80 g of Schisandra chinensis, wash them separately, mix them, add 18.6 kg of purified water, and perform ultrasonic extraction at 110°C for 105 min at an ultrasonic frequency of 2 kHz. After the extraction, filter out the residue and collect the filtrate. Then, add 18.6 kg of purified water to the residue again, perform ultrasonic extraction at 110°C for 90 min at an ultrasonic frequency of 2 kHz. After the extraction, filter out the residue and collect the filtrate. Combine the filtrates obtained from the two extractions and vacuum concentrate them at a pressure of -0.08 to -0.10 MPa and a temperature of 60-70°C to 1 / 35 of the original total volume to obtain a Chinese medicine aqueous extract.

[0060] (2) Mix the prepared Chinese herbal medicine water extract, edible vinegar and purified water according to the above weight proportions, then filter and collect the filtrate.

[0061] (3) The prepared supernatant is bottled and sterilized, and then ultra-high temperature instantaneous sterilization is performed at 125°C for 3 seconds to obtain the liver-protecting traditional Chinese medicine composite health vinegar.

[0062] Comparative Example 1 This comparative example provides a dilution of edible vinegar, the preparation method of which is as follows: (1) Vinegar and purified water were mixed in a mass ratio of 2:1, and then centrifuged at 1500 r / min for 10 min, and the supernatant was collected.

[0063] (2) The prepared supernatant is bottled and sterilized by ultra-high temperature instantaneous sterilization at 125°C for 3 seconds.

[0064] Comparative Example 2 This comparative example provides a dilution of a Chinese medicine aqueous extract, the preparation method of which is as follows: (1) Prepare the Chinese medicine aqueous extract according to the method of Example 1.

[0065] (2) The prepared Chinese medicine water extract and purified water were mixed in a mass ratio of 3:2, and then centrifuged at 1500 r / min for 10 min, and the supernatant was collected.

[0066] (3) The prepared supernatant is bottled and sterilized by ultra-high temperature instantaneous sterilization at 125°C for 3 seconds.

[0067] Comparative Example 3 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese medicinal materials used to prepare the Chinese herbal aqueous extract do not contain Pueraria root, and the weight of Hovenia dulcis, White Peony Root, and Schisandra chinensis are all 1000 g.

[0068] Comparative Example 4 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese medicinal materials used to prepare the Chinese herbal aqueous extract do not contain white peony root, and the weight of Pueraria root, Hovenia dulcis fruit and Schisandra chinensis are all 1000 g.

[0069] Comparative Example 5 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese medicinal materials used to prepare the Chinese herbal aqueous extract do not contain Hovenia dulcis, and the weight of Pueraria root, White Peony Root and Schisandra chinensis is 1000 g.

[0070] Comparative Example 6 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese medicinal materials used to prepare the Chinese herbal aqueous extract do not contain Schisandra chinensis, and the weight of Pueraria lobata, Hovenia dulcis and Paeonia lactiflora are all 1000 g.

[0071] Comparative Example 7 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese medicinal materials used to prepare the Chinese herbal aqueous extract do not contain Pueraria lobata and White Peony Root, and the weight of Hovenia dulcis and Schisandra chinensis are both 1500 g.

[0072] Comparative Example 8 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese medicinal materials used to prepare the Chinese herbal aqueous extract do not contain Hovenia dulcis and Schisandra chinensis, and the weight of Pueraria lobata and White Peony Root are both 1500 g.

[0073] Comparative Example 9 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese herbal medicine used to prepare the Chinese herbal medicine aqueous extract is only white peony root, weighing 3000 g.

[0074] Comparative Example 10 This comparative example provides a Chinese herbal composite health-care vinegar, the raw materials and preparation method of which are basically the same as those in Example 1, except that the Chinese herbal medicine used to prepare the Chinese herbal medicine aqueous extract is only Pueraria root, weighing 3000 g.

[0075] Test Example 1 The same batch of Chinese medicinal materials and edible vinegar were used to prepare liver-protecting Chinese medicinal composite health-care vinegar according to the preparation methods of Examples 1 to 5, an edible vinegar dilution was prepared according to the preparation method of Comparative Example 1, a Chinese medicinal water extract dilution was prepared according to the preparation method of Comparative Example 2, and a Chinese medicinal composite health-care vinegar was prepared according to the preparation methods of Comparative Examples 3 to 10. The protective effect of the vinegar on acute liver injury in mice was studied.

[0076] 1. Establishment of alcoholic liver injury model Two hundred and sixteen male ICR mice (18-22 g) were acclimated to a constant temperature and humidity (22 ± 2°C, 45%-65% RH) indoor environment for one week before the experiment. Mice were randomly divided into 18 groups (12 mice / group). The specific groups are shown in Table 1. Each group received the corresponding test sample by oral gavage daily. The blank and model control groups received distilled water. The dose was calculated at 10 times the human dose. Animals were weighed twice weekly, and the dose of the test sample was adjusted based on body weight. The specific doses are shown in Table 1. After 30 days of administration of the test sample, each group received 50% v / v ethanol (14 mL / kg) by oral gavage. The blank control group received distilled water. After fasting for 16 hours, the eyes were enucleated and blood was collected. The animals were then sacrificed by cervical dislocation. Blood samples and a portion of liver tissue were stored at −80°C for analysis and histopathological examination. A portion of liver tissue was sectioned and stored at −20°C for Oil Red O staining. All animal experiments were performed in accordance with the guidelines of the institutional animal ethics committee.

[0077] Table 1 Animal groups and dosage

[0078] 2. Oil Red O staining of animal liver Liver fixation and staining: Thoroughly mix 6 parts saturated Oil Red O staining solution with 4 parts distilled water. Incubate at 4°C overnight. The next day, filter once with qualitative filter paper, incubate at 4°C for 24 hours, and filter again to obtain the Oil Red O working solution. Remove frozen sections from the -20°C freezer and return to room temperature. Fix with tissue fixative for 15 minutes, rinse with sterile water, and air-dry. Stain sections in the Oil Red O working solution for 8–10 minutes (covered and protected from light). Remove sections, hold for 3 seconds, and then immerse in two separate containers of 60% isopropanol for 3 seconds and 5 seconds, respectively, for differentiation. Rinse sections in two separate containers of pure water for 10 seconds each. Remove sections, hold for 3 seconds, and then counterstain with hematoxylin for 3–5 minutes. Rinse in three separate containers of pure water for 5 seconds, 10 seconds, and 30 seconds, respectively. Differentiation solution was used for 2-8 seconds, followed by 2 washes in distilled water for 10 seconds each, followed by 1 second of blueing solution. The sections were then gently immersed in 2 washes of tap water for 5 and 10 seconds each, and the staining was examined microscopically. The sections were mounted with glycerol-gelatin mounting media. Microscopic examination and image acquisition and analysis were performed. Figure 2 shown.

[0079] Depend on Figure 2 As can be seen, compared with the blank control group, the red area of ​​the liver tissue of the model control group mice was obvious, and the relative area of ​​lipid droplets was significantly increased, indicating that the model was successful. Compared with the model control group, the fatty degeneration of the liver cells of the mice in the positive control group and Example 1 to Example 5 groups was significantly alleviated, and the lipid droplet vacuoles were reduced. Although the comparative examples 1 to 10 groups had different degrees of improvement, they were not as good as the examples. At the same time, the improvement effect of Example 1 on liver fatty degeneration was the most significant, better than the positive control group, which shows that the Chinese herbal compound health vinegar prepared by Example 1 showed the best effect in inhibiting alcohol-induced liver damage.

[0080] 3. Biochemical index detection Serum index detection: Prepare a 2 mL sterile EP tube, collect blood and centrifuge to aspirate the supernatant, centrifuge 3 times to obtain serum. Then, follow the instructions of the kit to determine the AST and ALT levels in the serum. The results are shown in the table. Figure 3 .

[0081] Liver marker test: Frozen liver tissue samples were taken out of the -80°C freezer and returned to room temperature. They were mixed with 0.9% saline at a ratio of 1:9 and ground at low temperature to prepare a homogenate. Subsequently, the homogenate was centrifuged at 3000 rpm / min for 10 min at 4°C. The TG and GSH contents in the liver were calculated according to the instructions of the kit. The results were as follows: Figure 3 shown.

[0082] Depend on Figure 3 It can be seen that compared with the blank control group, the various indicators (ALT, AST, TG and GSH) in the model control group changed significantly (P < 0.05), indicating that the model was successfully constructed and the mouse liver was damaged.

[0083] The serum ALT and AST levels of mice in Examples 1 to 5 were significantly lower than those in the model control group, while there were no significant differences between the Comparative Examples 1 to 10 groups and the model control group. Testing of liver TG and GSH levels revealed that TG and GSH levels were effectively improved in the mice in Examples 1 to 5 and the positive control group, particularly in the groups of Examples 1 and 2, which were significantly better than the positive control group in terms of lowering TG and increasing GSH levels. There were no significant differences between the comparative example groups and the model control group.

[0084] The above results all indicate that the liver-protecting Chinese medicinal compound health-care vinegar provided by the present invention can effectively alleviate liver damage, reduce liver lipid deposition, and improve antioxidant capacity, thereby significantly alleviating liver damage caused by alcohol.

[0085] Test Example 2 1. Storage stability observation A liver-protecting traditional Chinese medicine composite health-care vinegar was prepared according to the preparation method of Example 1, and the storage stability was investigated.

[0086] The liver-protecting Chinese medicinal composite health-care vinegar prepared according to the preparation method of Example 1 was placed in equal amounts in a glass sealed clean transparent container and stored under different temperature conditions and observed. Two typical storage temperatures of 4°C and 25°C were selected. The vinegar was photographed and observed on the 1st, 8th, 15th and 22nd day, respectively. The results are shown in FIG. Figure 4 .

[0087] Depend on Figure 4 It can be seen that after storage at different temperature conditions of 4°C and 25°C for 1 to 4 weeks, the prepared Chinese herbal compound health vinegar had no obvious color change, no abnormal foam or scum was produced, and no stratification or precipitation was observed, which indicates that the Chinese herbal compound health vinegar may have good stability.

[0088] 2. Determination of total acid content stability The same batch of Chinese medicinal materials and edible vinegar was used to prepare a liver-protecting Chinese medicinal composite health-care vinegar according to the preparation method of Example 1, and the stability of the total acid content was examined.

[0089] Equal amounts of the liver-protecting traditional Chinese medicine composite health-care vinegar prepared according to the preparation method of Example 1 were placed in a sealed, clean, transparent glass container and stored at 4°C and 25°C, respectively. Samples were taken on the first, eighth, fifteenth, and twenty-second days for total acid content determination. 10.0 mL of the sample was placed in a 100 mL volumetric flask, water was added to the mark, and the mixture was mixed. 20.0 mL of the sample was then placed in a 200 mL beaker, and 60 mL of water was added. The solution was titrated with 0.05 mol / L sodium hydroxide standard solution to a pH of 8.2 on the pH meter. The milliliters of sodium hydroxide standard solution consumed were recorded to calculate the total acid content. A blank test was performed using distilled water. The calculation formula is as follows: Where: X is the total acid content in the sample, g / 100 mL; V1 is the volume of sodium hydroxide standard solution consumed by the sample, mL; V2 is the volume of sodium hydroxide standard solution consumed by the blank, mL; V = sample volume, mL; and C is the concentration of the sodium hydroxide standard solution, mol / L. See the results Figure 5 .

[0090]

[0091] Depend on Figure 5 As can be seen, the total acid content showed a downward trend with extended storage time at both 4°C and 25°C. It decreased from 7.75 to 7.61 g / mL at 4°C and from 8.03 to 7.84 g / mL at 25°C, but neither temperature change reached a significant level. Furthermore, the total acid content at 25°C was higher than that at 4°C, but there was no significant difference over the same number of days. Both storage temperatures, 4°C and 25°C, exhibited high stability, effectively maintaining the relative stability of the total acid content in the samples.

[0092] 3. Determination of antioxidant DPPH activity stability The same batch of Chinese medicinal materials and edible vinegar were used to prepare a liver-protecting Chinese medicinal composite health-care vinegar according to the preparation method of Example 1, and the stability of its antioxidant DPPH activity was examined.

[0093] Equal amounts of the liver-protecting traditional Chinese medicine composite health-care vinegar prepared according to the preparation method of Example 1 were placed in a sealed, clean, transparent glass container and stored at 4°C and 25°C, respectively. Samples were taken on the 1st, 8th, 15th, and 22nd days for DPPH activity assay using a kit. A Trolox standard curve was first prepared by diluting the Trolox solution with PBS to standard solutions of 0.15, 0.3, 0.6, 0.9, 1.2, and 1.5 mmol / L. 180 μL of DPPH working solution was added to all wells. Except for the control wells, which received 20 μL of PBS solution, 20 μL of the diluted standard solution was added to the remaining wells. The 96-well plate was covered and gently shaken to mix thoroughly. The plate was incubated at room temperature in the dark for 30 minutes. A517 was detected and the free radical scavenging rate of each concentration of the standard solution was calculated. A standard curve (Y = 55.87X - 3.940, R = 0.0000) was then plotted with the Trolox standard solution concentration as the horizontal axis and the DPPH free radical scavenging rate as the vertical axis. 2 =0.9953). The sample was then diluted 50-fold with PBS solution, 180 μL of DDPPH working solution was added to the 96-well plate, and then 20 μL of the diluted sample was added. The above steps were followed and the results were expressed as Trolox equivalents. Figure 6 .

[0094] Depend on Figure 6 As can be seen, at both storage temperatures (4°C and 25°C), DPPH radical scavenging activity showed a decreasing trend with prolonged storage, ranging from 54.92-52.71 Mm Trolox / L at 4°C and 58.59-55.45 Mm Trolox / L at 25°C, but the changes at either temperature did not reach a significant level. However, although slightly higher DPPH radical scavenging activity was observed at 25°C throughout the storage period, this change did not reach a significant level compared to 4°C, indicating that the antioxidant stability of the samples was maintained at both temperatures.

[0095] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A liver-protecting Chinese medicine composite health-care vinegar, characterized in that: The raw materials include, by weight, 28 to 32 parts of a Chinese herbal medicine aqueous extract and 13 to 40 parts of edible vinegar. The Chinese herbal medicine raw materials for preparing the Chinese herbal medicine aqueous extract include kudzu root, hovenia dulcis fruit, white peony root and schisandra chinensis, and the mass ratio of the kudzu root, hovenia dulcis fruit, white peony root and schisandra chinensis is (1.7 to 2.3): (0.8 to 1.2): (1.7 to 2.3): (0.8 to 1.2).

2. The liver-protecting Chinese medicine composite health-care vinegar according to claim 1, characterized in that: The mass ratio of the Chinese herbal medicine aqueous extract to the edible vinegar is 30:13; and / or The mass ratio of Pueraria root, Hovenia dulcis fruit, White Peony Root and Schisandra chinensis is 2:1:2:1; and / or The edible vinegar is Shanxi aged vinegar with an acidity between 7 and 8; and / or The raw materials of the liver-protecting traditional Chinese medicine composite health-care vinegar also include 7 to 20 parts of purified water; and / or The raw materials of the liver-protecting traditional Chinese medicine compound health-care vinegar also include flavoring agents.

3. The liver-protecting Chinese medicine composite health-care vinegar according to claim 1 or 2, characterized in that: The preparation method of the Chinese medicine aqueous extract is as follows: the Chinese medicine raw material is ultrasonically extracted 2 to 3 times with purified water at 90 to 110° C., the amount of purified water used each time is 25 to 30 times the mass of the Chinese medicine raw material, the time of each ultrasonic extraction is 90 to 105 minutes, the extracts obtained from each extraction are combined and concentrated to 1 / 35 to 1 / 45 of the original total volume to obtain the Chinese medicine aqueous extract.

4. The liver-protecting Chinese medicine composite health-care vinegar according to claim 3, characterized in that: The temperature of the ultrasonic extraction is 100°C; and / or The ultrasonic extraction is performed twice, each time for 90 minutes; and / or The ultrasonic extraction has an ultrasonic frequency of 2 to 4 kHz; and / or The concentration temperature is 60-70°C; and / or Concentrate to 1 / 40 of the original total volume.

5. The liver-protecting Chinese medicine composite health-care vinegar according to claim 4, characterized in that: The ultrasonic extraction has an ultrasonic frequency of 3 kHz; and / or The concentration method is vacuum concentration at a pressure of -0.08 to -0.10 MPa.

6. The method for preparing the liver-protecting Chinese medicinal composite health-care vinegar according to any one of claims 1 to 5, characterized in that: The specific steps include: S1, mixing the raw materials by weight, removing the solid precipitate, and collecting the clear liquid; S2. Bottle and sterilize the clear liquid to obtain the liver-protecting Chinese medicinal compound health-care vinegar.

7. The preparation method according to claim 6, characterized in that The method of removing the solid precipitate in S1 is centrifugation or filtration; and / or The sterilization method described in S2 is ultra-high temperature instantaneous sterilization at 125°C for 3 seconds.

8. Use of the liver-protecting traditional Chinese medicine composite health-care vinegar according to any one of claims 1 to 5 in the preparation of liver-protecting products.

9. The use according to claim 8, characterized in that The liver protection product is a product for preventing or alleviating acute liver damage caused by alcohol.

10. The use according to claim 8, characterized in that The liver protection product is a health food that has an auxiliary protective effect on chemical liver damage.

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