A 20K SNP liquid phase breeding chip for pea whole genome and its application

By developing a 20K SNP liquid-phase breeding chip for the whole pea genome, the problems of high cost and insufficient detection flexibility in pea breeding have been solved, enabling efficient breeding and genotype detection, and promoting the innovation of pea varieties and the development of agricultural production.

CN120536614BActive Publication Date: 2026-03-17XIANGHU LABORATORY
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Patent Information

Application Number
CN202510528625.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2025-01-17
Filing Date
2025-04-25
Publication Date
2026-03-17
Estimated Expiration
2045-04-25

AI Technical Summary

Technical Problem

Existing technologies in pea breeding suffer from high resequencing costs and insufficient detection flexibility of traditional solid-phase chips, limiting the application of large-genome species and resulting in low breeding efficiency.

Method used

A 20K SNP liquid-phase breeding chip for the whole pea genome was developed, containing 21,659 high-quality SNP loci. Utilizing high-throughput sequencing and liquid-phase hybridization capture technologies, it is suitable for platforms such as Illumina and MGI, enabling efficient, rapid, and low-cost genotyping detection.

Benefits of technology

It improves the efficiency of pea breeding, promotes variety innovation and improvement, and is applicable to important trait analysis, germplasm resource genetic diversity analysis, variety identification, assisted selection breeding, population structure analysis, genetic and evolutionary analysis, and kinship identification.

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Abstract

This invention discloses a pea genome-wide 20K SNP liquid-phase breeding chip and its application. This chip contains 21,659 SNP loci from the pea genome, specifically based on agronomic trait association analysis and whole-genome resequencing data from pea samples, along with resequencing data from various varieties. High-quality, representative SNP loci of 21,659 were selected from these data. This chip can be widely used for pea genotyping, contributing to improved pea breeding efficiency and pea variety innovation.
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