Prebiotic sea-buckthorn fiber fruit drink with function of remarkably regulating intestinal flora

Abstract

The invention discloses a prebiotic sea-buckthorn fiber fruit drink with a function of remarkably regulating intestinal flora. The prebiotic sea-buckthorn fiber fruit drink comprises water, a sorbitol solution, sea-buckthorn primary pulp and prebiotics, the prebiotics are prepared from L-arabinose, xylooligosaccharide, galactooligosaccharide, fructo-oligosaccharide, galactomannan and inulin. According to the invention, the sorbitol liquid, the hippophae rhamnoides puree and the specific prebiotics are combined, and through component complementation, metabolite synergy and ecological niche optimization, a virtuous circle of inhibiting harmful bacteria, proliferating beneficial bacteria and improving a metabolic environment is formed, so that the intestinal flora balance is maintained, and the intestinal health is improved. Meanwhile, the relative abundance of beneficial bacteria such as lactic acid bacteria, ackermann bacteria, bifidobacterium and bacteroides can be remarkably improved; finally, dynamic balance of intestinal flora is achieved, and the synergistic effects of improving the intestinal microecology, enhancing the digestion function and improving the whole body health are achieved.

Description

Technical Field

[0001] The invention relates to a prebiotic seabuckthorn fiber fruit drink, in particular to a prebiotic seabuckthorn fiber fruit drink with a significant function of regulating intestinal flora. Background Art

[0002] With the public's growing interest in healthy lifestyles, market demand for functional foods and beverages has seen significant growth. This trend has driven the continuous introduction of new and innovative health drinks to meet consumers' diverse health needs, including boosting immunity, providing antioxidants, improving digestion, and regulating blood sugar and lipids.

[0003] Seabuckthorn is a nutrient-dense fruit rich in vitamin C, antioxidants, various minerals, and dietary fiber, making it widely used in health foods. Research has confirmed that seabuckthorn fruit not only significantly enhances immunity, provides antioxidant benefits, and improves digestion, but also effectively promotes metabolism and regulates blood sugar and lipid levels.

[0004] Prebiotics are a class of non-digestible substances that cannot be digested or absorbed by the human body but selectively stimulate the growth and reproduction of beneficial intestinal bacteria, improving the intestinal microecological environment and thereby benefiting host health. As "invisible guardians of intestinal health," prebiotics play a vital role in chronic disease prevention and immune enhancement through multiple mechanisms, including targeted regulation of the microbiome and improved metabolism. As consumers' understanding of "microecological health" deepens, the application of prebiotics in functional foods is becoming increasingly diverse.

[0005] At present, there are no reports on the research of combining prebiotics with sea buckthorn puree to make fruit drinks. Summary of the Invention

[0006] The object of the present invention is to provide a prebiotic sea buckthorn fiber fruit drink which is a combination of sea buckthorn puree and specific prebiotics and has a significant function of regulating intestinal flora.

[0007] The present invention is implemented by the following technical scheme: a prebiotic sea buckthorn fiber fruit drink with a significant function of regulating intestinal flora, comprising a composition and a prebiotic, wherein 15-28 g of the prebiotic is added to every 100 ml of the composition; wherein the composition comprises the following components in parts by volume: 70-90 parts of water, 4-6 parts of sorbitol solution, and 10-40 parts of sea buckthorn puree; and the prebiotic is composed of L-arabinose, xylooligosaccharides, galacto-oligosaccharides, fructo-oligosaccharides, galactomannan, and inulin.

[0008] Sorbitol liquid, sea buckthorn puree and a specific prebiotic combination work synergistically to achieve a double improvement in health and taste: the specific prebiotic combination leads to the proliferation of beneficial bacteria, the part of sorbitol liquid not absorbed by the small intestine assists in fermentation with sea buckthorn dietary fiber, jointly increasing the production of short-chain fatty acids and strengthening intestinal flora regulation; sea buckthorn antioxidant components and short-chain fatty acids maintain the intestinal mucosa; sorbitol liquid, prebiotic combination and sea buckthorn organic acids synergistically improve bowel movements and digestion; sea buckthorn nutrients combine with short-chain fatty acids to build an antioxidant network and activate immunity; at the same time, sorbitol liquid neutralizes the sour and astringent taste of sea buckthorn and reduces calories, and its moisturizing properties and sea buckthorn colloid optimize the texture, avoiding viscosity problems caused by prebiotics.

[0009] The combination of sorbitol liquid, sea buckthorn puree and prebiotics achieves the synergistic effects of improving intestinal microecology, enhancing digestive function and improving overall health through the triple mechanism of "sweetness regulation-microbial flora regulation-antioxidation".

[0010] Furthermore, the prebiotics are composed of the following components in parts by mass: 5 to 9 parts of L-arabinose, 1 to 6 parts of xylo-oligosaccharides, 2 to 5 parts of galacto-oligosaccharides, 1.5 to 3.5 parts of fructo-oligosaccharides, no more than 3 parts of galactomannan and 0.5 to 1.5 parts of inulin.

[0011] Advantages of the present invention: The combination of sorbitol liquid, sea buckthorn puree and prebiotics in the present invention forms a virtuous cycle of "inhibiting harmful bacteria - proliferating beneficial bacteria - improving the metabolic environment" through component complementarity, metabolic product synergy and niche optimization, ultimately achieving a dynamic balance of intestinal flora. The prebiotic sea buckthorn fiber fruit drink of the present invention can maintain the balance of intestinal flora, while significantly increasing the relative abundance of beneficial bacteria such as Lactobacillus, Akkermansia, Bifidobacterium and Bacteroides. The combination of sorbitol liquid, sea buckthorn puree and prebiotics in the present invention achieves a synergistic effect of improving intestinal microecology, enhancing digestive function and improving overall health through the triple mechanism of "sweetness regulation - flora regulation - anti-oxidation". DETAILED DESCRIPTION

[0012] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0013] Example 1: A prebiotic sea buckthorn fiber fruit drink with a significant function of regulating intestinal flora, comprising a composition and a prebiotic, with 15 g of prebiotic added to every 100 ml of the composition; wherein the composition comprises the following components in parts by volume: 80 parts of water, 4.5 parts of sorbitol solution, and 20 parts of sea buckthorn puree; the prebiotic is composed of the following components in parts by mass: 8 parts of L-arabinose, 2.5 parts of xylo-oligosaccharides, 2 parts of galacto-oligosaccharides, 1.5 parts of fructo-oligosaccharides, 0.5 parts of galactomannan, and 0.5 parts of inulin.

[0014] The method for preparing the prebiotic sea buckthorn fiber fruit drink of this embodiment is as follows: sea buckthorn puree, sorbitol solution, water, L-arabinose, xylooligosaccharides, galacto-oligosaccharides, fructo-oligosaccharides, galactomannan, and inulin are measured in the above proportions and mixed evenly to prepare the prebiotic sea buckthorn fiber fruit drink. The sea buckthorn puree of this embodiment is prepared using conventional methods.

[0015] Example 2: A prebiotic sea buckthorn fiber fruit drink with a significant function of regulating intestinal flora, comprising a composition and a prebiotic, with 20 g of prebiotic added to every 100 ml of the composition; wherein the composition comprises the following components in parts by volume: 70 parts of water, 4 parts of sorbitol solution, and 10 parts of sea buckthorn puree; the prebiotic is composed of the following components in parts by mass: 5 parts of L-arabinose, 6 parts of xylo-oligosaccharides, 3 parts of galacto-oligosaccharides, 2 parts of fructo-oligosaccharides, 2 parts of galactomannan, and 1 part of inulin.

[0016] Example 3: A prebiotic sea buckthorn fiber fruit drink with a significant function of regulating intestinal flora, comprising a composition and a prebiotic, with 28 g of prebiotic added to every 100 ml of the composition; wherein the composition comprises the following components in parts by volume: 90 parts of water, 6 parts of sorbitol solution, and 40 parts of sea buckthorn puree; 28 g of prebiotic is added to every 100 ml of the composition of water and sea buckthorn puree; the prebiotic is composed of the following components in parts by mass: 9 parts of L-arabinose, 3 parts of xylo-oligosaccharides, 5 parts of galactooligosaccharides, 3.5 parts of fructooligosaccharides, 3 parts of galactomannan, and 1.5 parts of inulin.

[0017] Comparative Example 1: The difference from Example 1 is that no prebiotics were added, and the other preparation methods and raw material ratios were exactly the same as those in Example 1.

[0018] Comparative Example 2: The difference from Example 1 is that the prebiotics are composed of the following components by weight: 8 parts of L-arabinose, 2.5 parts of oligoxylose, and 0.5 parts of inulin. The other preparation methods and raw material ratios are exactly the same as those in Example 1.

[0019] Comparative Example 3: The difference from Example 1 is that the prebiotics are composed of the following components by weight: 2.5 parts of xylo-oligosaccharide, 2 parts of galacto-oligosaccharide, and 0.5 parts of inulin. The other preparation methods and raw material ratios are exactly the same as those in Example 1.

[0020] Comparative Example 4: The difference from Example 1 is that the prebiotics are composed of the following components by weight: 8 parts of L-arabinose, 2.5 parts of oligoxylose, and 0.5 parts of galactomannan. The other preparation methods and raw material ratios are exactly the same as those in Example 1.

[0021] Comparative Example 5: The difference from Example 1 is that the prebiotics are composed of the following components by weight: 4 parts fucoidan, 3 parts chitosan oligosaccharide, 2 parts galacto-oligosaccharide, 1.5 parts fructo-oligosaccharide, 0.5 parts galactomannan, and 0.5 parts polydextrose. The other preparation methods and raw material ratios are exactly the same as in Example 1.

[0022] To investigate the regulatory effects of a prebiotic seabuckthorn fiber drink on intestinal flora, this study used 90 SPF mice aged 10 to 12 weeks, with stable weight and mature physiological status. After one week of adaptive feeding in a standard environment with a temperature of 20-24°C, a humidity of 50-60%, and a 12-hour light and 12-hour dark cycle, the mice were randomly divided into nine groups of 10 mice each, designated as experimental groups 1 to 3, control groups 1 to 5, and blank group 1. Experimental groups 1 to 3 were administered with the prebiotic seabuckthorn fiber drink of Examples 1 to 3, respectively; control groups 1 to 5 were administered with the prebiotic seabuckthorn fiber drink of Comparative Examples 1 to 5, respectively; and blank group 1 was administered with normal saline.

[0023] During the trial, each group received a 10 mL / kg oral gavage at 10:00 AM daily for four weeks. After the trial, mouse feces were collected and placed in sterile EP tubes. The relative abundance of Lactobacillus, Bifidobacterium, Bacteroides, Akkermansia, Escherichia coli, and Klebsiella in the feces was determined. The results are shown in Table 1. Table 1: Test results of test groups 1 to 3, control groups 1 to 5, and blank group 1

[0024] The relative abundance of microorganisms refers to the proportion of a certain type or category of microorganisms to the total number of microorganisms in a microbial community (such as intestinal flora, soil microorganisms, water microorganisms, etc.).

[0025] The experimental results showed that compared with the control groups 1 to 5 and the blank group 1, the prebiotic sea buckthorn fiber fruit drink in the experimental groups 1 to 3 could maintain the balance of intestinal flora, and significantly increased the relative abundance of beneficial bacteria such as Lactobacillus, Akkermansia, Bifidobacterium and Bacteroides, and reduced the relative abundance of harmful bacteria.

[0026] The present invention comprises a combination of sea buckthorn puree, sorbitol solution, and specific prebiotics including L-arabinose, xylooligosaccharides, galacto-oligosaccharides, fructo-oligosaccharides, galactomannan, and inulin, which can maintain the balance of intestinal flora, significantly increase the relative abundance of beneficial bacteria such as Lactobacillus, Akkermansia, Bifidobacterium, and Bacteroides, and reduce the relative abundance of harmful bacteria. The principle lies in the synergistic effect of sea buckthorn puree, sorbitol solution, and specific prebiotic combination: sea buckthorn puree is rich in bioactive ingredients such as polyphenols, flavonoids, vitamins, and dietary fiber, which can inhibit the growth of harmful bacteria and create a suitable environment for beneficial bacteria through antibacterial, anti-inflammatory, and antioxidant effects, as well as regulating intestinal pH. The specific prebiotic combination of the present invention has a high degree of flora selectivity, can be preferentially metabolized by beneficial bacteria such as Lactobacillus and Bifidobacterium, promotes the proliferation of beneficial bacteria, and enhances the intestinal barrier function. The portion of sorbitol not absorbed by the small intestine can reach the large intestine as an additional fermentation substrate, thereby increasing the production of short-chain fatty acids. Through ingredient complementarity, metabolic product synergy and niche optimization, the three form a virtuous cycle of "inhibiting harmful bacteria - proliferating beneficial bacteria - improving the metabolic environment", and ultimately achieve a dynamic balance of intestinal flora.

[0027] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A prebiotic seabuckthorn fiber fruit drink with significant function of regulating intestinal flora, characterized in that: The invention comprises a composition and prebiotics, wherein 15-28 g of the prebiotics are added to every 100 ml of the composition; wherein the composition comprises the following components in parts by volume: 70-90 parts of water, 4-6 parts of sorbitol solution, and 10-40 parts of sea buckthorn puree; and the prebiotics are composed of L-arabinose, xylooligosaccharides, galacto-oligosaccharides, fructo-oligosaccharides, galactomannan, and inulin.

2. The prebiotic sea buckthorn fiber fruit drink having a significant function of regulating intestinal flora according to claim 1, characterized in that: The prebiotics are composed of the following components in parts by mass: 5-9 parts of L-arabinose, 1-6 parts of xylo-oligosaccharides, 2-5 parts of galacto-oligosaccharides, 1.5-3.5 parts of fructo-oligosaccharides, no more than 3 parts of galactomannan and 0.5-1.5 parts of inulin.

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