A method for treating a triclosan antibacterial coating on sutures

Abstract

The present application relates to the technical field of antibacterial coating, in particular to a processing method of triclosan antibacterial coating for suture, and the specific steps are as follows: S1, preparation of moxa tablet-triclosan antibacterial solution; S2, preparation of moxa tablet-triclosan spraying solution; S3, suture pretreatment; S4, suture spraying.The present application has the following beneficial effects: 1. Good versatility: the suture with moxa tablet-triclosan antibacterial coating prepared by the present application is sprayed by ultrasonic spraying machine, and the same production process is used for different suture materials and single or multi-strand sutures, so that the production cost is greatly reduced, and the development of enterprises is facilitated; 2. Broad-spectrum bacteriostasis: the suture has good bacteriostatic rate for staphylococcus aureus, escherichia coli and stenotrophomonas maltophilia; 3. Low allergenicity.

Description

Technical Field

[0001] This invention relates to the field of antibacterial coating technology, specifically a method for treating a triclosan antibacterial coating on sutures. Background Technology

[0002] Surgical sutures are specialized sutures used in surgery to close wounds, suture tissues, and ligate blood vessels. They can be classified into various types based on their characteristics, and their main function is to provide mechanical support for wound healing.

[0003] Based on absorbency, sutures can be divided into two main categories: absorbable sutures and non-absorbable sutures.

[0004] Absorbable suture materials include PDO (polydioxanone), PGCL (polyglycolic acid-caprolactone copolymer), PGLA (polyglycolic acid-lactide copolymer), and PGA (polyglycolic acid).

[0005] Non-absorbable suture materials include PVDF (polyvinylidene fluoride), PTFE (polytetrafluoroethylene), PP (polypropylene), PET (polyester fiber), and UHMWPE (ultra-high molecular weight polyethylene).

[0006] In addition, sutures can also be classified according to their structure into single-strand sutures and multi-strand sutures.

[0007] A Chinese patent (application number: 20151035225.5) discloses an antibacterial surgical suture and its preparation method, which includes the following steps:

[0008] (1) Dissolve triclosan in double-distilled water to prepare a base solution with a triclosan mass concentration of 0.8%; (2) Place polypropylene resin, polyglycolic acid, polylactide and polyglycolic acid-lactide in a reaction vessel and melt them at a temperature of 380℃ and a pressure of 2.4MPa. Then dry and pulverize them into powder with a particle size of 1500 mesh; (3) Add the powder obtained in step (2) to the base solution in step (1) and stir and evaporate it to a viscous paste at a temperature of 116℃ and a rotation speed of 1300 rpm; (4) Place the paste obtained in step (3) in a spinneret, and after spinning, seal and store it to obtain antibacterial surgical sutures.

[0009] The above preparation method has the following technical problems in actual production:

[0010] 1. Lack of versatility: Different suture materials have different raw materials and different adhesion properties, which requires adjusting the proportion of adhesion enhancers, solubilizers and other components in the triclosan solution. If you want to produce sutures with different raw materials, the production cost will increase linearly, which is not conducive to the development of enterprises.

[0011] 2. Limited application: The sutures produced by the above preparation methods are all single strands. For multi-strand sutures, subsequent weaving is required, which is complicated. In addition, the triclosan coating inevitably peels off during the weaving process, which seriously affects the antibacterial effect of the coating. Summary of the Invention

[0012] The purpose of this invention is to provide a method for treating a triclosan antibacterial coating on sutures, in order to solve the problems mentioned in the background art.

[0013] To achieve the above objectives, the present invention provides the following technical solution: a method for treating a triclosan antibacterial coating on sutures, comprising the following specific steps:

[0014] Preparation of S1, Artemisia annua-triclosan antibacterial solution

[0015] S11. By mass fraction, take 10-15 parts of acetylene tablets, 5-7 parts of pyridine, 5-10 parts of triclosan and 250-300 parts of tetrahydrofuran into a reaction vessel, stir at 0-5℃ for 30-60 min, then heat the reaction vessel to 20-25℃ and react for 24 h to obtain the reaction solution.

[0016] S12. Filter the reaction solution to obtain a filtrate, and then rotary evaporate the filtrate to obtain an oily substance.

[0017] S13. Dissolve the oily substance in 20 times its mass fraction of anhydrous diethyl ether to obtain Ai Pian-Triclosan antibacterial solution;

[0018] Preparation of S2, Artemisia annua-triclosan spray solution

[0019] By mass fraction, take 100-120 parts of the aforementioned antibacterial solution of acetic acid-triclosan, 5-10 parts of film-forming resin, 1-2 parts of solubilizer and 0.5-1 parts of adhesion enhancer, melt and mix them evenly to obtain the acetic acid-triclosan spray solution.

[0020] S3, Suture pretreatment

[0021] The sutures to be coated are cleaned with plasma to remove surface grease and improve coating adhesion.

[0022] S4, Spraying of sutures

[0023] Using an ultrasonic spraying machine, the acetaminophen-triclosan spraying liquid is atomized into micron-sized particles by a high-pressure pump and then sprayed onto the pre-treated sutures.

[0024] Further, step S11 is as follows: Take 12 parts of acetaminophen, 6 parts of pyridine, 8 parts of triclosan and 280 parts of tetrahydrofuran by mass into a reaction vessel, stir at 0-5℃ for 40 min, then heat the reaction vessel to 20-25℃ and react for 24 h to obtain the reaction solution.

[0025] Further, step S2 is as follows: by mass, take 110 parts of the acetaminophen-triclosan antibacterial liquid, 8 parts of film-forming resin, 1.5 parts of solubilizer and 0.8 parts of adhesion enhancer, melt and mix them evenly to obtain the acetaminophen-triclosan spray liquid.

[0026] Furthermore, in step S3, the material for the suture to be sprayed can be either absorbable or non-absorbent.

[0027] Furthermore, in step S3, the material of the suture to be sprayed is selected from absorbable single-strand PDO, absorbable single-strand PGCL, absorbable multi-strand PGLA, or absorbable multi-strand PGA.

[0028] Furthermore, in step S3, the material of the suture to be sprayed is selected from non-absorbent single-strand PVDF, non-absorbent single-strand PTFE, non-absorbent single-strand PP, non-absorbent multi-strand PET, or non-absorbent multi-strand UHMWPE.

[0029] This invention provides a method for treating a triclosan antibacterial coating on sutures, comprising the following:

[0030] Beneficial effects:

[0031] 1. Good versatility: The suture with the antibacterial coating of acetaminophen-triclosan obtained by this invention is produced by ultrasonic spraying. The same production process is used for different suture materials and single or multi-strand sutures, which greatly reduces the production cost and is beneficial to the development of enterprises.

[0032] 2. Broad-spectrum antibacterial activity: The sutures coated with acetaminophen-triclosan antibacterial agent prepared by the method of this invention have a good antibacterial rate against Staphylococcus aureus, Escherichia coli and Stenotrophomonas maltophilia. In contrast, traditional sutures with triclosan antibacterial agent coating have a poor antibacterial effect against Stenotrophomonas maltophilia. Stenotrophomonas maltophilia is a non-fermenting Gram-negative bacterium, a common pathogenic Gram-negative bacterium that is common in nosocomial infections and is naturally resistant to many antibiotics.

[0033] 3. Low sensitization: The sutures with the arugula-triclosan antibacterial agent coating prepared by this invention showed lower sensitization in mice compared to the sutures with the borneol-triclosan composite antibacterial agent coating. Detailed Implementation

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention.

[0035] Example 1

[0036] A method for applying a triclosan antibacterial coating to sutures, comprising the following steps:

[0037] Preparation of S1, Artemisia annua-triclosan antibacterial solution

[0038] S11. By mass fraction, take 10 parts of acetylene, 5 parts of pyridine, 5 parts of triclosan and 250 parts of tetrahydrofuran into a reaction vessel, stir at 0-5℃ for 30 min, then heat the reaction vessel to 20-25℃ and react for 24 h to obtain the reaction solution.

[0039] S12. Filter the reaction solution to obtain a filtrate, and then rotary evaporate the filtrate to obtain an oily substance.

[0040] S13. Dissolve the oily substance in 20 times its mass fraction of anhydrous diethyl ether to obtain Ai Pian-Triclosan antibacterial solution;

[0041] Preparation of S2, Artemisia annua-triclosan spray solution

[0042] By mass fraction, take 100 parts of the above-mentioned antibacterial solution of acetic acid-triclosan, 5 parts of film-forming resin, 1 part of solubilizer and 0.5 parts of adhesion enhancer, melt and mix them evenly to obtain the acetic acid-triclosan spray solution;

[0043] S3, Suture pretreatment

[0044] The sutures to be coated are cleaned with plasma to remove surface grease and improve coating adhesion.

[0045] S4, Spraying of sutures

[0046] Using an ultrasonic spraying machine, the acetaminophen-triclosan spraying liquid is atomized into micron-sized particles by a high-pressure pump and then sprayed onto the pre-treated sutures.

[0047] In practice, the artemisia tablets are made from the leaf extract of Artemisia argyi, a plant of the Asteraceae family. It belongs to the natural borneol branch and has the advantage of mature synthesis technology. The cost is only 1 / 10 of that of borneol. At the same time, since the artemisia tablets are of natural plant origin, they are less irritating than synthetic borneol. The sensitization of borneol mainly comes from its strong transdermal properties and impurities in synthetic products. Artemisia tablets of natural plant origin have fewer impurities and higher purity after purification, and the risk of allergies is lower than that of borneol.

[0048] Furthermore, in step S3, the material for the suture to be sprayed can be either absorbable or non-absorbent.

[0049] Furthermore, in step S3, the material of the suture to be sprayed is selected from absorbable single-strand PDO, absorbable single-strand PGCL, absorbable multi-strand PGLA, or absorbable multi-strand PGA.

[0050] Furthermore, in step S3, the material of the suture to be sprayed is selected from non-absorbent single-strand PVDF, non-absorbent single-strand PTFE, non-absorbent single-strand PP, non-absorbent multi-strand PET, or non-absorbent multi-strand UHMWPE.

[0051] It should be noted that sutures can be classified into two main categories based on their absorbency: absorbable sutures and non-absorbable sutures. Absorbable sutures are made of materials such as PDO (polydioxanone), PGCL (polyglycolic acid-caprolactone copolymer), PGLA (polyglycolic acid-lactide copolymer), and PGA (polyglycolic acid). Non-absorbable sutures are made of materials such as PVDF (polyvinylidene fluoride), PTFE (polytetrafluoroethylene), PP (polypropylene), PET (polyester fiber), and UHMWPE (ultra-high molecular weight polyethylene).

[0052] In addition, sutures can also be classified according to their structure into single-strand sutures and multi-strand sutures.

[0053] Example 2

[0054] A method for applying a triclosan antibacterial coating to sutures, comprising the following steps:

[0055] Preparation of S1, Artemisia annua-triclosan antibacterial solution

[0056] S11. By mass fraction, take 15 parts of acetaminophen, 7 parts of pyridine, 10 parts of triclosan and 300 parts of tetrahydrofuran into a reaction vessel, stir at 0-5℃ for 60 min, then heat the reaction vessel to 20-25℃ and react for 24 h to obtain the reaction solution.

[0057] S12. Filter the reaction solution to obtain a filtrate, and then rotary evaporate the filtrate to obtain an oily substance.

[0058] S13. Dissolve the oily substance in 20 times its mass fraction of anhydrous diethyl ether to obtain Ai Pian-Triclosan antibacterial solution;

[0059] Preparation of S2, Artemisia annua-triclosan spray solution

[0060] By mass fraction, take 120 parts of the above-mentioned antibacterial solution of acetic acid-triclosan, 10 parts of film-forming resin, 2 parts of solubilizer and 1 part of adhesion enhancer, melt and mix them evenly to obtain the acetic acid-triclosan spray solution;

[0061] S3, Suture pretreatment

[0062] The sutures to be coated are cleaned with plasma to remove surface grease and improve coating adhesion.

[0063] S4, Spraying of sutures

[0064] Using an ultrasonic spraying machine, the acetaminophen-triclosan spraying liquid is atomized into micron-sized particles by a high-pressure pump and then sprayed onto the pre-treated sutures.

[0065] In practice, the artemisia tablets are made from the leaf extract of Artemisia argyi, a plant of the Asteraceae family. It belongs to the natural borneol branch and has the advantage of mature synthesis technology. The cost is only 1 / 10 of that of borneol. At the same time, since the artemisia tablets are of natural plant origin, they are less irritating than synthetic borneol. The sensitization of borneol mainly comes from its strong transdermal properties and impurities in synthetic products. Artemisia tablets of natural plant origin have fewer impurities and higher purity after purification, and the risk of allergies is lower than that of borneol.

[0066] Furthermore, in step S3, the material for the suture to be sprayed can be either absorbable or non-absorbent.

[0067] Furthermore, in step S3, the material of the suture to be sprayed is selected from absorbable single-strand PDO, absorbable single-strand PGCL, absorbable multi-strand PGLA, or absorbable multi-strand PGA.

[0068] Furthermore, in step S3, the material of the suture to be sprayed is selected from non-absorbent single-strand PVDF, non-absorbent single-strand PTFE, non-absorbent single-strand PP, non-absorbent multi-strand PET, or non-absorbent multi-strand UHMWPE.

[0069] It should be noted that sutures can be classified into two main categories based on their absorbency: absorbable sutures and non-absorbable sutures. Absorbable sutures are made of materials such as PDO (polydioxanone), PGCL (polyglycolic acid-caprolactone copolymer), PGLA (polyglycolic acid-lactide copolymer), and PGA (polyglycolic acid). Non-absorbable sutures are made of materials such as PVDF (polyvinylidene fluoride), PTFE (polytetrafluoroethylene), PP (polypropylene), PET (polyester fiber), and UHMWPE (ultra-high molecular weight polyethylene).

[0070] In addition, sutures can also be classified according to their structure into single-strand sutures and multi-strand sutures.

[0071] Example 3

[0072] A method for applying a triclosan antibacterial coating to sutures, comprising the following steps:

[0073] Preparation of S1, Artemisia annua-triclosan antibacterial solution

[0074] S11. By mass fraction, take 12 parts of acetaminophen, 6 parts of pyridine, 8 parts of triclosan and 280 parts of tetrahydrofuran into a reaction vessel, stir at 0-5℃ for 40 min, then heat the reaction vessel to 20-25℃ and react for 24 h to obtain the reaction solution.

[0075] S12. Filter the reaction solution to obtain a filtrate, and then rotary evaporate the filtrate to obtain an oily substance.

[0076] S13. Dissolve the oily substance in 20 times its mass fraction of anhydrous diethyl ether to obtain Ai Pian-Triclosan antibacterial solution;

[0077] Preparation of S2, Artemisia annua-triclosan spray solution

[0078] By mass, 110 parts of the aforementioned antibacterial solution of acetic acid-triclosan, 8 parts of film-forming resin, 1.5 parts of solubilizer and 0.8 parts of adhesion enhancer are melted and mixed evenly to obtain the acetic acid-triclosan spray solution.

[0079] S3, Suture pretreatment

[0080] The sutures to be coated are cleaned with plasma to remove surface grease and improve coating adhesion.

[0081] S4, Spraying of sutures

[0082] Using an ultrasonic spraying machine, the acetaminophen-triclosan spraying liquid is atomized into micron-sized particles by a high-pressure pump and then sprayed onto the pre-treated sutures.

[0083] In practice, the artemisia tablets are made from the leaf extract of Artemisia argyi, a plant of the Asteraceae family. It belongs to the natural borneol branch and has the advantage of mature synthesis technology. The cost is only 1 / 10 of that of borneol. At the same time, since the artemisia tablets are of natural plant origin, they are less irritating than synthetic borneol. The sensitization of borneol mainly comes from its strong transdermal properties and impurities in synthetic products. Artemisia tablets of natural plant origin have fewer impurities and higher purity after purification, and the risk of allergies is lower than that of borneol.

[0084] Furthermore, in step S3, the material for the suture to be sprayed can be either absorbable or non-absorbent.

[0085] Furthermore, in step S3, the material of the suture to be sprayed is selected from absorbable single-strand PDO, absorbable single-strand PGCL, absorbable multi-strand PGLA, or absorbable multi-strand PGA.

[0086] Furthermore, in step S3, the material of the suture to be sprayed is selected from non-absorbent single-strand PVDF, non-absorbent single-strand PTFE, non-absorbent single-strand PP, non-absorbent multi-strand PET, or non-absorbent multi-strand UHMWPE.

[0087] It should be noted that sutures can be classified into two main categories based on their absorbency: absorbable sutures and non-absorbable sutures. Absorbable sutures are made of materials such as PDO (polydioxanone), PGCL (polyglycolic acid-caprolactone copolymer), PGLA (polyglycolic acid-lactide copolymer), and PGA (polyglycolic acid). Non-absorbable sutures are made of materials such as PVDF (polyvinylidene fluoride), PTFE (polytetrafluoroethylene), PP (polypropylene), PET (polyester fiber), and UHMWPE (ultra-high molecular weight polyethylene).

[0088] In addition, sutures can also be classified according to their structure into single-strand sutures and multi-strand sutures.

[0089] Comparative Example 1

[0090] The sutures have a coating of borneol-triclosan composite antibacterial agent on the surface.

[0091] Comparative Example 2

[0092] The sutures have an antibacterial coating of mugwort extract on the surface.

[0093] Comparative Example 3

[0094] The sutures have a triclosan antibacterial coating on the surface.

[0095] Staphylococcus aureus, Escherichia coli, and Stenotrophomonas maltophilia were placed in liquid culture medium and incubated for 24 hours. Then, 1 cm sections of the six types of sutures from the three examples and three comparative examples were cut, forming three groups. These groups were placed in liquid culture medium containing Staphylococcus aureus, Escherichia coli, and Stenotrophomonas maltophilia, respectively, sealed with sealing film, and incubated at 30°C for 72 hours. The results are shown in the table below.

[0096]

[0097] As shown in the table above, when used alone, Artemisia annua tablets do not have a broad-spectrum antibacterial and bactericidal effect, and have almost no antibacterial rate against Staphylococcus aureus, Escherichia coli, and Stenotrophomonas maltophilia.

[0098] The sutures have a triclosan antibacterial coating. Stenotrophomonas maltophilia is a non-fermenting Gram-negative bacterium, a common pathogenic Gram-negative bacterium, frequently causing nosocomial infections. It is naturally resistant to many antibiotics, and triclosan has a poor inhibitory effect on it.

[0099] The sutures coated with a borneol-triclosan composite antibacterial agent have a good antibacterial rate against Staphylococcus aureus, Escherichia coli and Stenotrophomonas maltophilia.

[0100] The sutures coated with the antibacterial agent triclosan and prepared by the method of this invention also have a good antibacterial rate against Staphylococcus aureus, Escherichia coli and Stenotrophomonas maltophilia.

[0101] Meanwhile, the sutures with an antibacterial coating of arbutin-triclosan prepared by this invention showed lower sensitization in mice compared to sutures with a borneol-triclosan composite antibacterial coating.

Claims

1. A method for treating triclosan antimicrobial coating of sutures, characterized by, The specific steps are as follows: S1, preparation of the moxa tablet-triclosan antibacterial solution S11, according to the mass fraction, take moxa tablet 10-15 parts, pyridine 5-7 parts, triclosan 5-10 parts and tetrahydrofuran 250-300 parts in the reaction kettle, stir at 0-5℃ for 30-60min, then heat the reaction kettle to 20-25℃ and react for 24h to obtain the reaction liquid; S12, filter the reaction liquid to obtain the filtrate, and distill the filtrate to obtain an oil; S13, dissolve the oil in 20 times its mass fraction of anhydrous ether to obtain the moxa tablet-triclosan antibacterial solution; S2, preparation of the moxa tablet-triclosan spraying solution According to the mass fraction, take the moxa tablet-triclosan antibacterial solution 100-120 parts, film-forming resin 5-10 parts, solubilizing agent 1-2 parts and adhesion enhancer 0.5-1 parts, melt and mix uniformly to obtain the moxa tablet-triclosan spraying solution; S3, suture pretreatment The suture to be sprayed is cleaned by plasma to remove surface grease and improve coating adhesion; S4, suture spraying Use an ultrasonic spraying machine to atomize the coating liquid into micron-sized particles by high-pressure pump, and spray the pretreated suture with the coating liquid.

2. A method for treating suture with triclosan antimicrobial coating according to claim 1, wherein Step S11 is as follows: according to the mass fraction, take moxa tablet 12 parts, pyridine 6 parts, triclosan 8 parts and tetrahydrofuran 280 parts in the reaction kettle, stir at 0-5℃ for 40min, then heat the reaction kettle to 20-25℃ and react for 24h to obtain the reaction liquid.

3. A method for treating suture with triclosan antimicrobial coating according to claim 1, wherein Step S2 is as follows: according to the mass fraction, take the moxa tablet-triclosan antibacterial solution 110 parts, film-forming resin 8 parts, solubilizing agent 1.5 parts and adhesion enhancer 0.8 parts, melt and mix uniformly to obtain the moxa tablet-triclosan spraying solution.

4. A method of treating suture with triclosan antimicrobial coating according to claim 1, wherein: The material of the suture to be sprayed in step S3 is selected from absorbable materials or non-absorbable materials.

5. A method of treating a suture with a triclosan antimicrobial coating according to claim 4, wherein: The material of the suture to be sprayed in step S3 is selected from absorbable single-strand PDO, absorbable single-strand PGCL, absorbable multi-strand PGLA or absorbable multi-strand PGA.

6. A method of treating a suture with a triclosan antimicrobial coating according to claim 4, wherein: The material of the suture to be sprayed in step S3 is selected from non-absorbable single-strand PVDF, non-absorbable single-strand PTFE, non-absorbable single-strand PP, non-absorbable multi-strand PET or non-absorbable multi-strand UHMWPE.

Images