Fluorescence imaging equipment and application

Abstract

The invention belongs to the technical field of biological imaging, and particularly relates to fluorescence imaging equipment and application. The fluorescence imaging equipment comprises a rack, an optical machine module and a detection platform, the optical machine module and the detection platform are both arranged on the rack, and the detection platform is used for loading a carrier for containing a sample; the ray machine module is arranged to be capable of selecting the excitation light beams with different wavelengths to be incident on the sample in the carrier so as to excite the imaging light beams, and the imaging light beams are matched and filtered according to the excitation light beams so as to perform imaging. The functional module for emitting the excitation light beam and the functional module for receiving the fluorescence for imaging in the fluorescence imaging equipment are both integrated in the ray machine module, so that richer application scenes can be ensured, and higher integration level can also be ensured.

Description

Technical Field

[0001] The present application belongs to the field of biological imaging technology, and in particular relates to a fluorescence imaging device and its application. Background Art

[0002] Fluorescence imaging is a commonly used biotechnology method for observing and analyzing samples labeled with fluorescent dyes or fluorescent proteins. Fluorescence imaging utilizes the properties of fluorescent substances, which emit fluorescent signals of specific wavelengths under excitation light. Using an image acquisition module, the intensity, distribution, and dynamic changes of the fluorescent signals can be observed and recorded, enabling analysis of the sample.

[0003] Fluorescence imaging has a wide range of applications in biomedical research, drug development, molecular biology, and cell biology.

[0004] At present, fluorescence imaging equipment generally includes a laser illumination function module and an imaging function module. The laser illumination function module is generally used to emit laser light to excite the sample to form a fluorescence signal, and the imaging module is generally used to receive the fluorescence signal for imaging. The laser illumination function module and the imaging function module are the main functional modules forming the imaging optical path system in the fluorescence imaging equipment.

[0005] Currently, some fluorescence imaging devices on the market rely on a laser illumination module and an imaging module to excite and receive only a single fluorescent signal, limiting their application scenarios. While some fluorescence imaging devices can excite and receive multiple fluorescent signals, these modules require more complex structures and are often separated, resulting in a low level of integration. Summary of the Invention

[0006] The present application provides a fluorescence imaging device to solve the technical problem that existing devices are difficult to meet the requirements of high integration in a wide range of application scenarios.

[0007] According to one aspect of the present application, a fluorescence imaging device is provided, including a frame, an optical-mechanical module and a detection platform. The optical-mechanical module and the detection platform are both arranged on the frame, and the detection platform is used to load a carrier for holding samples; the optical-mechanical module is configured to be able to select excitation light beams of different wavelengths to be incident on the sample in the carrier to excite the imaging light beam, and to filter the imaging light beam according to the matching of the excitation light beam to form an image.

[0008] In an optional solution of the present application, the optical machine module includes an excitation source module, a fluorescence imaging module and an optical path splitting module; the fluorescence imaging module and the optical path splitting module are arranged at intervals in the direction of the optical path where the imaging beam is located, and the excitation source module is located on the peripheral side of the optical path where the imaging beam is located; the excitation source module is configured to be able to selectively emit excitation beams of different wavelengths, and the optical path splitting module can reflect the excitation beam to be incident on the sample in the carrier; the fluorescence imaging module is used to match and filter the imaging beam incident through the optical path splitting module according to the excitation beam to form an image.

[0009] In an optional solution of the present application, the excitation source module includes a light source switching mechanism and at least two light emitting units; each light emitting unit is connected to the light source switching mechanism, and the light source switching mechanism is used to select any one of the multiple light emitting units to emit an excitation light beam.

[0010] In an optional solution of the present application, the fluorescence imaging module includes an image acquisition unit, a filter switching mechanism and multiple first filters; the multiple first filters are all arranged in the filter switching mechanism, and the filter switching mechanism can select any first filter to be located in the optical path of the imaging beam; the image acquisition unit is used to receive the filtered imaging beam for imaging.

[0011] In an optional solution of the present application, the filter switching mechanism includes a filter holder, a first driving member and a positioning unit; the filter holder is provided with a plurality of through holes, the plurality of through holes include a plurality of fluorescent holes and at least one bright field hole, and each first filter is arranged in the corresponding fluorescent hole; the first driving member is connected to the filter holder to drive the filter holder to move, and the positioning unit is used to determine the movement position of the filter holder, and cooperate with the first driving member to select any through hole to be located on the optical path of the imaging beam.

[0012] In an optional solution of the present application, the optical machine module also includes a white light source, which is located between the optical path splitting module and the detection platform in the direction of the optical path of the imaging beam; the white light source is used to emit white light toward the sample in the carrier.

[0013] In an optional solution of the present application, the optical machine module also includes an optical machine base plate, and the optical path splitting module includes an optical lens; the optical machine base plate is provided with an optical path through hole, and the optical path through hole is located on the optical path of the imaging light beam, and the optical path splitting module and the white light source are respectively arranged on opposite sides of the optical machine base plate; the white light source is arranged around the optical path through hole, and the projection of the optical lens in the direction of the optical path where the imaging light beam is located overlaps with the optical path through hole.

[0014] In an optional solution of the present application, the optical machine module also includes a protective cover, which is arranged on the optical machine base plate and covers the optical path splitting module; the excitation source module is arranged on the optical machine base plate and is located on the peripheral side of the protective cover, the fluorescence imaging module is arranged on the top side plate of the protective cover, and the filter fixing part of the fluorescence imaging module is located inside the protective cover.

[0015] In an optional solution of the present application, the optical machine module also includes a guide seat, which is detachably connected to the peripheral side plate of the protective cover; the guide seat is located between the excitation source module and the protective cover, and the guide seat is provided with a guide groove and an excitation beam through-hole, and the excitation beam through-hole is located in the guide groove; the guide groove accommodates the light emitting end of each light emitting unit in the excitation source module, and the light source switching mechanism in the excitation source module can drive the light emitting unit to move along the guide groove, and allow the light emitting end of any light emitting unit to be aligned with the excitation beam through-hole, and the excitation beam through-hole is used to guide the excitation beam to the optical path splitting module.

[0016] In an optional solution of the present application, the detection platform includes a multi-axis combination slide and a sample supporting plate, and the sample supporting plate is used to hold the sample carrier; the sample supporting plate is arranged on the multi-axis combination slide, and the multi-axis combination slide is used to adjust the position of the sample supporting plate to change the position of the carrier.

[0017] According to another aspect of the present application, the application of the fluorescence imaging device in plant gene editing is protected.

[0018] In summary, the fluorescence imaging device provided by this application has at least the following beneficial effects:

[0019] The fluorescence imaging device includes a frame, an optical machine module and a detection platform. The detection platform can load a carrier containing samples, and the frame supports the optical machine module, the detection platform, etc.

[0020] The sample here has a fluorescent factor, and the optical machine module can emit an excitation beam toward the sample. The excitation beam can excite the fluorescent factor in the sample to produce fluorescence, and the optical machine module can also receive the fluorescence for imaging, that is, the fluorescence here is an imaging beam.

[0021] The type of fluorescent factor in the sample can be selected based on requirements. Different types of fluorescent factors require different wavelengths of excitation beam to trigger fluorescence. The optical machine module can select the appropriate excitation beam to generate fluorescence based on the type of fluorescent factor. At the same time, the optical machine module can also perform matched filtering based on the generated fluorescence to improve imaging quality.

[0022] It can be seen that the fluorescence imaging device can adapt to the use of different types of fluorescent factors, which greatly enriches the application scenarios. In addition, the functional module for emitting the excitation light beam and the functional module for receiving fluorescence for imaging in the fluorescence imaging device are both integrated in the optical machine module, which can ensure richer application scenarios and a higher degree of integration. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] To more clearly illustrate the specific embodiments of this application or the technical solutions in the prior art, the following briefly introduces the drawings required for the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be derived from these drawings without inventive effort.

[0024] Figure 1 A schematic diagram of a fluorescence imaging device equipped with a carrier according to one embodiment of the present application;

[0025] Figure 2 for Figure 1 Schematic diagram of the optical engine module;

[0026] Figure 3 for Figure 2 The optical-mechanical module in the figure hides the excitation source module and some sheet metal parts from another perspective;

[0027] Figure 4 for Figure 2 Schematic diagram of the excitation source module;

[0028] Figure 5 for Figure 2 Schematic diagram of the fluorescence imaging module;

[0029] Figure 6 for Figure 1 Schematic diagram of the detection platform;

[0030] Figure 7 for Figure 1 Schematic diagram of the fluorescence imaging device in another perspective;

[0031] Figure 8 for Figure 7 Schematic diagram of the fluorescence imaging device in the unfolded state.

[0032] The reference numerals are as follows:

[0033] 1000. Fluorescence imaging equipment;

[0034] 100, rack; 110, movable mounting plate; R, storage room;

[0035] 200, optical machine module;

[0036] 210, excitation source module; 211, light source switching mechanism; 2111, second driving member; 2112, support base; 2113, guide rail; 2114, slider; 2115, screw transmission unit; 212, light emitting unit; 2121, emission head; 2122, second filter;

[0037] 220, fluorescence imaging module; 221, image acquisition unit; 2211, camera; 2212, lens; 222, filter switching mechanism; 2221, filter fixing member; 2222, first driving member; 2223, positioning unit; 2224, positioning structure; 223, first filter; E, notch; H1, through hole; H11, fluorescence hole; H12, bright field hole;

[0038] 230, optical path splitting module; 231, optical lens; 232, lens mounting frame; 233, adjusting screw assembly;

[0039] 240, white light source; 250, optical machine base; 260, protective cover; 270, guide seat; 280, cover; C, guide groove; H2, light path hole; H3, excitation beam hole; H4, cable hole;

[0040] 300, testing platform; 310, multi-axis combined slide; 320, sample carrier plate; D, limit slot;

[0041] 400, vehicle;

[0042] 511. Power module; 512. White light source controller; 513. Excitation light source controller; 514. Drive device controller; 515. Power interface; 516. Communication interface; 517. Cooling fan. DETAILED DESCRIPTION

[0043] In the description of this application, it should be understood that if terms such as "center", "vertical", "horizontal", "top", "bottom", "inside", "outside", "axial", "radial", and "circumferential" appear to indicate orientation or positional relationships, unless otherwise specified, they are understood to be based on the orientation or positional relationships shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on this application.

[0044] Furthermore, the use of "first" or "second" in describing features is for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features identified. Features identified as "first" or "second" may explicitly or implicitly include at least one of the identified features. The use of the word "plurality" generally implies at least two, such as two or three, unless otherwise specifically defined.

[0045] In this application, unless otherwise specified or limited, terms such as "mounted," "connected," "connect," and "fixed" should be interpreted broadly. For example, they can refer to fixed connections, removable connections, or integration; mechanical connections, electrical connections, direct connections, or indirect connections through an intermediary; and internal connections between two components or interactions between two components. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.

[0046] In the description of this specification, if the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" appear, it means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and the features of different embodiments or examples without contradiction.

[0047] Some existing fluorescence imaging devices can select the appropriate excitation beam to stimulate the corresponding fluorescence signal based on the type of fluorescent factor in the sample. In other words, these existing fluorescence imaging devices can use a variety of laser sources to adapt to different fluorescent factors, which greatly enriches application scenarios and can achieve better imaging results.

[0048] In existing fluorescence imaging devices, the optical path system is primarily composed of a laser illumination module and an imaging module. The inventors discovered that to achieve a more complex optical path system to enrich application scenarios, the laser illumination module and imaging module in existing fluorescence imaging devices are both complex in structure and employ a split layout, resulting in a low level of integration.

[0049] To this end, the present application provides a fluorescent imaging device 1000 that is compatible with high integration in a variety of application scenarios. Figure 1 Schematic diagram of a fluorescence imaging device 1000 provided with a carrier 400 according to one embodiment of the present application.

[0050] It should be noted that the "X direction," "Y direction," and "Z direction" mentioned in the embodiments of the present application are interpreted with reference to the coordinate system constructed by the fluorescence imaging device 1000. The X direction and the Y direction intersect perpendicularly and form a horizontal direction parallel to the ground, and the Z direction is perpendicular to the horizontal direction.

[0051] See also Figure 1 The fluorescence imaging device 1000 includes a frame 100, an optical machine module 200 and a detection platform 300. The optical machine module 200 and the detection platform 300 are both arranged on the frame 100. The detection platform 300 is used to load a carrier 400 for holding samples.

[0052] The optical engine module 200 is configured to select excitation beams of different wavelengths to be incident on the sample in the carrier 400 to excite the imaging beam, and to match and filter the imaging beam according to the excitation beam to form an image.

[0053] In this embodiment, the optical machine module 200 , the detection platform 300 and some other components can be integrated on the frame 100 to form the fluorescence imaging device 1000 .

[0054] The frame 100 may be assembled from a variety of profiles, sheet metal parts, etc. The optical machine module 200 is used to form an optical path for detecting samples in the carrier 400 , and the detection platform 300 is used to load the carrier 400 .

[0055] It should be noted that the sample in the carrier 400 has a fluorescent factor, which can generate an imaging beam under the action of an excitation beam. The imaging beam here is the fluorescence generated by the fluorescent factor in the sample being excited.

[0056] It is understandable that the types of fluorescent factors in the sample can be configured according to needs, and different types of fluorescent factors require excitation light beams of different wavelengths to stimulate fluorescence.

[0057] The optical-mechanical module 200 selects an appropriate excitation beam based on the type of fluorescence factor to be incident on the sample in the carrier 400, thereby generating an imaging beam corresponding to that fluorescence factor. Furthermore, the optical-mechanical module 200 can also perform matched filtering on the imaging beam stimulated by the excitation beam to improve imaging quality.

[0058] It can be seen that the fluorescence imaging device 1000 can be adapted to different types of fluorescence factors, and the functional module for selecting the excitation light beam and the functional module for matching the filtered imaging light beam in the fluorescence imaging device 1000 are both integrated in the optical machine module 200, which can ensure rich application scenarios and also ensure a compact structure and high integration.

[0059] It should be noted that the carrier 400 here can be a biochip, a culture dish, a multi-well plate, etc., which can be selected according to application requirements.

[0060] exist Figure 1In the illustrated embodiment, the optical machine module 200 is located above the detection platform 300 in the Z direction. The excitation light beam emitted by the optical machine module 200 can be vertically incident on the carrier 400 on the detection platform 300 along the Z direction. The imaging light beam formed by the sample in the carrier 400 can be vertically incident on the optical machine module 200 along the Z direction and captured by the optical machine module 200 for imaging.

[0061] Figure 2 for Figure 1 Schematic diagram of the optical machine module 200. It should be noted that in order to facilitate the observation of the internal structure of the optical machine module 200, Figure 2 The optical engine module 200 shown hides some sheet metal covering parts.

[0062] See also Figure 2 The optical machine module 200 includes an excitation source module 210, a fluorescence imaging module 220, and an optical path splitting module 230. The fluorescence imaging module 220 and the optical path splitting module 230 are spaced apart in the optical path direction of the imaging beam, and the excitation source module 210 is located on the peripheral side of the optical path of the imaging beam.

[0063] The excitation source module 210 is configured to select excitation beams of different wavelengths and emit an excitation beam incident on the sample in the carrier 400. The fluorescence imaging module 220 is configured to match and filter the imaging beam incident via the optical path splitting module 230 according to the excitation beam to form an image.

[0064] In this embodiment, the excitation source module 210, the fluorescence imaging module 220, and the optical path splitting module 230 serve as the main components of the optical machine module 200. The excitation source module 210 is capable of selecting the desired excitation beam specifications, while the fluorescence imaging module 220 is capable of performing matched filtering based on the specifications of the imaging beam corresponding to the excitation beam.

[0065] Furthermore, the optical path splitting module 230 is configured to reflect the excitation beam while allowing the imaging beam to pass through. It should be understood that because the excitation beam must be reflected by the optical path splitting module 230 before being perpendicularly incident on the sample in the carrier 400, while the imaging beam is emitted from the sample in the carrier 400 and passes through the optical path splitting module 230 before being perpendicularly incident on the fluorescence imaging module 220, the excitation source module 210 and the fluorescence imaging module 220 are arranged at different positions within the optical path splitting module 230.

[0066] It can be seen that the excitation source module 210 , the fluorescence imaging module 220 and the light path segmentation module 230 are used to form a light path to perform fluorescence imaging on the sample in the carrier 400 to obtain fluorescence image data information.

[0067] exist Figure 2In the illustrated embodiment, the excitation source module 210 can emit an excitation light beam along the Y direction toward the optical path segmentation module 230. The excitation light beam is then reflected by the optical path segmentation module 230 and incident perpendicularly along the Z direction on the sample in the carrier 400. The imaging light beam formed by the excitation of the sample in the carrier 400 passes through the optical path segmentation module 230 along the Z direction and is incident perpendicularly on the fluorescence imaging module 220.

[0068] It can be seen that in the illustrated embodiment, the Z direction is the optical path direction of the imaging light beam, and the excitation source module 210 is located on the Y-direction side of the optical path splitting module 230 .

[0069] In a further optional embodiment, the optical machine module 200 further includes a white light source 240, which is located between the optical path splitting module 230 and the detection platform 300 in the direction of the imaging beam path. The white light source 240 is used to emit white light toward the sample in the carrier 400.

[0070] In this embodiment, the white light source 240 is used to emit white light. The white light can be reflected by the sample in the carrier 400 and enter the fluorescence imaging module 220 after passing through the light path splitting module 230 for imaging.

[0071] It can be seen that the white light emitted by the white light source 240 is a type of imaging light beam, and this white light cannot excite the sample in the carrier 400 to generate fluorescence.

[0072] In some usage scenarios, before fluorescence detection imaging, the white light source 240 can be used in conjunction with the fluorescence imaging module 220 to assist in locating the positions of the samples in the carrier 400 to facilitate subsequent operations.

[0073] Figure 3 for Figure 2 The optical machine module 200 in FIG. 2 is a schematic diagram showing the excitation source module 210 and some sheet metal parts hidden from another perspective. Figure 2 and Figure 3 The optical machine module 200 also includes an optical machine base plate 250 , and the optical path splitting module 230 includes an optical lens 231 .

[0074] The optical engine base plate 250 defines a light path hole H2 , which is located on the optical path of the imaging light beam. The light path splitting module 230 and the white light source 240 are respectively disposed on opposite sides of the optical engine base plate 250 .

[0075] The white light source 240 is arranged around the light path hole H2, and the projection of the optical lens 231 in the direction of the light path where the imaging light beam is located overlaps with the light path hole H2.

[0076] In this embodiment, the optical machine base plate 250 is used to provide support for the various component modules of the optical machine module 200. The optical path splitting module 230 and the white light source 240 are both arranged on the optical machine base plate 250, wherein the optical path splitting module 230 is located on the side away from the detection platform 300, and the white light source 240 is located on the side close to the detection platform 300.

[0077] The light path hole H2 on the optical engine base plate 250 is used for the excitation beam and the imaging beam to pass through. The optical lens 231 in the light path splitting module 230 plays the role of reflecting the excitation beam and transmitting the imaging beam.

[0078] It should be understood that the reflected excitation light beam is directed from the optical lens 231 toward the carrier 400, and the imaging light beam is directed from the carrier 400 toward the optical lens 231, so the two are in the same direction but in opposite directions. Since the excitation source module 210 is located on one side of the light path splitting module 230, and in order to ensure that the emitted excitation light beam and the imaging light beam are in the same direction and can pass through the light path hole H2, the optical lens 231 needs to be cross-tilted relative to the light path where the imaging light beam is located, and the size of the optical lens 231 can cover the projected area of ​​the light path formed at the optical lens 231 to ensure that the excitation light beam is completely reflected and the imaging light beam is completely passed through. Accordingly, the opening size of the light path hole H2 also needs to be appropriate and cannot block the light beam to ensure that the excitation light beam and the imaging light beam pass through completely.

[0079] In addition, the white light source 240 is arranged around the light path hole H2 , so that the white light source 240 is arranged in a ring shape. The emitted white light can evenly illuminate the carrier 400 and has a larger coverage area to illuminate the entire carrier 400 .

[0080] In a specific application, the optical lens 231 may be a dichroic mirror, a beam splitter, etc. The white light source 240 may be an annular light strip, or a plurality of white light lamps arranged at intervals along the circumference of the light path hole H2.

[0081] exist Figure 3 In the illustrated embodiment, the optical path splitting module 230 and the white light source 240 are arranged on opposite sides of the optical machine base plate 250 in the Z direction, and the acute angle formed between the optical lens 231 and the straight line in the Z direction is 45°, so as to ensure that the excitation light beam can be reflected to be vertically incident on the carrier 400 along the Z direction after being horizontally incident on the optical lens 231 along the Y direction.

[0082] In a further optional embodiment, the optical path splitting module 230 further includes a lens mounting frame 232 , and the optical lens 231 is disposed on the lens mounting frame 232 .

[0083] In this embodiment, the lens mounting frame 232 is used to fix and support the optical lens 231 and can make the angle between the optical lens 231 and the optical path of the imaging light beam appropriate, thereby ensuring that the reflected excitation light beam can be vertically incident on the carrier 400.

[0084] exist Figure 2 and Figure 3 In the illustrated embodiment, the lens mounting frame 232 is assembled from a plurality of sheet metal parts and cooperates with the optical lens 231 to cover the upper Z direction of the light path through hole H2. The optical lens 231 is fixedly mounted on the top side of the lens mounting frame 232. The acute angle formed by the optical lens 231 and the light path where the imaging light beam is located is 45°.

[0085] In specific applications, the optical lens 231 is provided with a three-point adjustment mechanism to achieve fine adjustment of the installation angle of the optical lens 231. Figure 2 and Figure 3 The optical lens 231 is provided with adjustment screw groups 233 on both opposite sides of the X direction. There is only one group of adjustment screw groups 233 in the middle position of one side of the X direction, and a group of adjustment screw groups 233 is provided at each end position of the other side of the X direction. In this way, a three-point adjustment mechanism is formed, and the installation angle of the optical lens 231 is precisely fine-tuned through a precision thread pair.

[0086] Figure 4 for Figure 2 Schematic diagram of the excitation source module 210. Figure 4 The excitation source module 210 includes a light source switching mechanism 211 and at least two light emitting units 212. Each light emitting unit 212 is connected to the light source switching mechanism 211, and the light source switching mechanism 211 is used to select any one of the plurality of light emitting units 212 to emit an excitation light beam.

[0087] In this embodiment, the excitation source module 210 includes multiple light emitting units 212, each capable of emitting an excitation light beam of a different wavelength. Furthermore, a light source switching mechanism 211 is configured to select one of the multiple light emitting units 212 to emit an excitation light beam of a corresponding wavelength toward the optical path splitting module 230. The excitation light beam of the corresponding wavelength is reflected by the optical path splitting module 230 and then incident on the carrier 400.

[0088] exist Figure 4 In the illustrated embodiment, the light source switching mechanism 211 is a linear drive module arranged along the X-direction to provide freedom of movement in that direction. Two light emitting units 212 are provided, each capable of emitting an excitation beam along the Y-direction. These two light emitting units 212 are arranged side by side in the X-direction and can be moved along the X-direction by the light source switching mechanism 211, thereby selecting one of the two light emitting units 212 to be positioned in the optical path of the excitation beam.

[0089] It should be understood that the number of light emitting units 212 is not limited to the illustrated embodiment and can be adjusted according to the specifications and quantity of the required excitation beams. Furthermore, the light source switching mechanism 211 is not limited to a linear transmission module and can also be, for example, a linear motor or a pneumatic cylinder to provide X-axis freedom of movement.

[0090] In a specific application, the excitation beam is a laser. It is understood that the laser can be provided by a fiber laser and transmitted via an optical fiber. A fiber laser can precisely output laser light of a specific wavelength. In an optional embodiment, the light emitting unit 212 includes a transmitter head 2121, which is used to connect to the output end of the optical fiber. The laser light output by the optical fiber is emitted through the transmitter head 2121.

[0091] Furthermore, the light emitting unit 212 further includes a second filter 2122 , which is disposed at the light emitting end of the emitting head 2121 .

[0092] It should be noted that the light emitting end of the transmitter head 2121 refers to the end of the transmitter head 2121 that can emit the excitation light beam. In this embodiment, each transmitter head 2121 is equipped with a second filter 2122 that can filter the corresponding wavelength. These second filters 2122 can avoid the influence of stray light and ensure that the excitation light beam is in the required wavelength band.

[0093] It should be understood that the number of light emitting units 212 is related to the types of laser beams that the fiber laser can emit. In specific applications, the fiber laser is a multimode fiber laser, with each module corresponding to a fiber outputting a specific type of laser beam. To accommodate the excitation source module 210 shown in the figure, the fiber laser used is a dual-mode fiber laser.

[0094] As can be seen, the excitation light beam emitted by the excitation source module 210 provided in this embodiment is a laser beam, which is easily adapted for use with a multi-mode laser. Each light emitting unit 212 is adapted to emit a laser beam of a specific wavelength, and the selection of the excitation light beam is achieved by changing the position of each light emitting unit 212 via the light source switching mechanism 211.

[0095] It should be noted that the excitation source module 210 is not limited to the illustrated embodiment. For example, the excitation source module 210 can introduce a broad spectrum beam and has a filter switching function unit, which selects different filters to filter the broad spectrum beam to select an excitation beam of a specific wavelength.

[0096] Figure 5 for Figure 2 Schematic diagram of the fluorescence imaging module 220. Figure 5In some optional embodiments, the fluorescence imaging module 220 includes an image acquisition unit 221, a filter switching mechanism 222, and a plurality of first filters 223. The plurality of first filters 223 are disposed within the filter switching mechanism 222, which can select any first filter 223 to be located on the optical path of the imaging beam. The image acquisition unit 221 is configured to receive the filtered imaging beam to generate an image.

[0097] In this embodiment, the image acquisition unit 221 is used to receive the imaging light beam and output image data to the host computer for imaging. All the first filters 223 are fixedly mounted on the filter switching mechanism 222. According to the wavelength of the imaging light beam excited by the excitation light beam, the filter switching mechanism 222 selects one of the multiple first filters 223 that can filter the imaging light beam of the wavelength and is located in the optical path of the imaging light beam to remove excess stray light and ensure imaging quality.

[0098] It should be understood that the specification type of the first filter 223 is determined by the specification type of the excitation beam, and the fluorescence imaging module 220 can select the corresponding first filter 223 through the filter switching mechanism 222 according to the specification of the excitation beam to achieve matching filtering of the imaging beam excited by the excitation beam of this specification.

[0099] In a specific application, the image acquisition unit 221 includes a camera 2211 and a lens 2212. The camera 2211 is used to collect the imaging beam to form an image, and the lens 2212 is used to adjust the observation angle. The camera 2211 can be an industrial camera, such as a CCD (Charge-Coupled Device) camera or a CMOS (Complementary Metal-Oxide-Semiconductor) camera. The lens 2212 includes, for example, a high-power lens or a zoom lens.

[0100] In a further optional embodiment, the filter switching mechanism 222 includes a filter holder 2221, a first driving member 2222, and a positioning unit 2223. The filter holder 2221 is provided with a plurality of through holes H1, the plurality of through holes H1 including a plurality of fluorescence holes H11 and at least one bright field hole H12, and each first filter 223 is disposed in a corresponding fluorescence hole H11.

[0101] The first driving member 2222 is connected to the filter holder 2221 to drive the filter holder 2221 to move. The positioning unit 2223 is used to determine the moving position of the filter holder 2221 to select any through hole H1 to be located on the optical path of the imaging beam.

[0102] In this embodiment, the filter holder 2221 is provided with a plurality of through holes H1. The number of through holes H1 is greater than the number of first filters 223. The portions of these through holes H1 where the first filters 223 are mounted serve as fluorescence holes H11, while the portions where no lenses are mounted serve as brightfield holes H12. Therefore, the number of first filters 223 and fluorescence holes H11 is the same.

[0103] As can be seen from the foregoing, the first filter 223 is used to filter the imaging light beam, so the fluorescent hole H11 can be used in conjunction with the excitation light beam, and the bright field hole H12 can be used in conjunction with the white light source 240.

[0104] The first driving member 2222 is drivingly connected to the filter holder 2221 to provide the filter holder 2221 with freedom of movement, so as to change the position of each through hole H1 and further adjust the position of each first filter 223 and the bright field hole H12.

[0105] The positioning unit 2223 can determine the reference position of the filter holder 2221. This reference position is the zero position, and the displacement of the filter holder 2221 relative to the zero position is the movement position of the filter holder 2221. Thus, when the first filter 223 or the brightfield aperture H12 of the required specifications in the filter holder 2221 is within the optical path of the imaging beam, the first driving member 2222 can be stopped to adapt the corresponding imaging beam.

[0106] It should be noted that the first driving member 2222 can directly drive the filter fixing member 2221 , or the first driving member 2222 can drive the filter fixing member 2221 via a transmission mechanism.

[0107] exist Figure 5 In the illustrated embodiment, the first driving member 2222 is a motor with a driving wheel fixedly mounted at its output end. The filter holder 2221 is a conversion wheel structure comprising at least a driven wheel. The conversion wheel structure is formed with a plurality of circumferentially spaced through holes H1. The driving and driven wheels are connected by a timing belt. In other words, the first driving member 2222 drives the filter holder 2221 to rotate via the timing belt, thereby switching the positions of the first filters 223 and the brightfield apertures H1.

[0108] In addition, a notch E is provided at the edge of the filter fixing member 2221, and the positioning unit 2223 is a slot-type photoelectric switch, and the notch E can move to the position where the slot-type photoelectric switch is located. Figure 5 It can be seen that when the notch E is not in the slot switch position, the optical signal in the slot photoelectric switch is blocked. When the notch E is in the slot photoelectric switch position, the optical signal in the slot photoelectric switch is not blocked, indicating that the filter holder 2221 is in the zero position.

[0109] Therefore, whether the filter holder 2221 is at zero position can be determined according to the switching signal of the slot-type photoelectric switch. Accordingly, the movement position of the filter holder 2221 can be determined by controlling the rotation angle of the first driving member 2222 .

[0110] It should be understood that the first driving member 2222 may be an incremental encoding motor. Thus, each time the fluorescence imaging device 1000 is started, the first driving member 2222 must cooperate with the positioning unit 2223 to calibrate the zero position of the filter holder 2221 .

[0111] Of course, the first drive member 2222 can also be an absolute encoder motor, which does not require zero calibration, and can directly drive the filter holder 2221. It should be understood that the speed of a direct motor drive is generally too fast, so using a synchronous belt drive can also reduce the speed of the filter holder 2221. In addition, the connection between the first drive member 2222 and the filter holder 2221 is not limited to being driven by a synchronous belt. For example, drive can also be achieved through a gear transmission system.

[0112] Of course, the positioning unit 2223 is not limited to a slot-type photoelectric switch, and may also be a proximity switch, a laser sensor, etc.

[0113] It should be noted that the filter switching mechanism 222 is not limited to an embodiment in which the plurality of through holes H1 are arranged in a ring and the filter holder 2221 is driven to rotate to switch any of the plurality of through holes H1 to the optical path of the imaging beam. For example, the plurality of through holes H1 on the filter holder 2221 may be arranged linearly and spaced apart in the horizontal direction, and the first driving member 2222 cooperates with the linear transmission mechanism to drive the filter holder 2221 to move linearly, thereby switching any of the plurality of through holes H1 to the optical path of the imaging beam.

[0114] Figure 5 The fluorescence imaging module 220 shown is used in conjunction with Figure 4 The excitation source module 210 shown is used. Since the excitation source module 210 can provide two types of excitation beam specifications, correspondingly, the first filter 223 on the filter holder 2221 also has two types of specifications.

[0115] exist Figure 5In the illustrated embodiment, there are six through holes H1, symmetrically arranged about the rotation center of the filter holder 2221. For example, the number of fluorescence holes H11 can be five, the number of brightfield holes H12 can be one, and the number of first filters 223 of two specifications can be five in total, installed in corresponding fluorescence holes H11. It should be understood that the number of fluorescence holes H11 and the number of brightfield holes H12 can be adjusted as needed. Furthermore, some of the through holes H1 can be used as spare holes in the event that the number or specifications of first filters 223 increases.

[0116] In a further optional embodiment, the filter switching mechanism 222 further includes a positioning structure 2224 , which is connected to the periphery of the filter fixing member 2221 to limit the filter fixing member 2221 .

[0117] In this embodiment, when the filter holder 2221 stops moving, the positioning structure 2224 ensures that the filter holder 2221 stops in a reliable position, that is, it prevents the filter holder 2221 from shaking, causing the selected through hole H1 position to deviate and affect the imaging effect.

[0118] exist Figure 5 In the illustrated embodiment, the positioning structure 2224 is a movable multi-link structure, one end of which is capable of being embedded in the tooth groove of the driven wheel in the filter holder 2221. When the filter holder 2221 rotates, the end of the movable multi-link structure pops out of the tooth groove without affecting the normal rotation of the filter holder 2221. When the filter holder 2221 stops rotating, the movable link structure returns to its original position, and accordingly, the end of the movable multi-link structure is embedded in the tooth groove, thereby defining the stop position of the filter holder 2221.

[0119] In a specific application, the movable connection structure includes a spring, and the reset is achieved by the spring. Of course, the positioning structure 2224 is not limited to this. For example, a telescopic electromagnet can also be used. When the filter holder 2221 stops rotating, the telescopic electromagnet extends into the tooth groove to achieve positioning.

[0120] See also Figure 2 and Figure 3 In some optional embodiments, the optical machine module 200 further includes a protective cover 260 , which is disposed on the optical machine base plate 250 and covers the optical path splitting module 230 .

[0121] The excitation source module 210 is disposed on the optical machine base plate 250 and located around the protective cover 260 . The fluorescence imaging module 220 is disposed on the top side plate of the protective cover 260 . The filter fixing member 2221 of the fluorescence imaging module 220 is located inside the protective cover 260 .

[0122] In this embodiment, the protective cover 260 is assembled from multiple panels and fixedly mounted on the optical engine base plate 250. The fluorescence imaging module 220 is fixedly mounted on the top side panels of the protective cover 260. Specifically, the image acquisition unit 221 and the filter holder 2221 in the fluorescence imaging module 220 are located on opposite sides of the top side panels of the protective cover 260. The filter holder 2221 is located on the side closer to the optical path splitting module 230 and within the protective cover 260, while the image acquisition unit 221 is located on the side farther from the optical path splitting module 230 and outside the protective cover 260.

[0123] In this way, the filter holder 2221 and the optical path splitting module 230 are both housed in the protective cover 260, so the protective cover 260 can protect the filter in the filter holder 2221 and the optical lens 231 in the optical path splitting module 230, thereby extending the service life of these lenses.

[0124] As can be seen from the above, after the imaging light beam passes through the optical path through hole H2, it passes through the optical lens 231 and is incident on the image acquisition unit 221 through the through hole H1 on the optical path. It can be seen that the optical lens 231, the filter fixing member 2221 and the image acquisition unit 221 are arranged in sequence in the direction of the optical path where the imaging light beam is located.

[0125] It should be understood that a lens hole (not shown) is provided on the top side panel of the protective cover 260 to cooperate with the through hole H1 and the light path hole H2 to form a passageway for the imaging light beam to pass through. In a specific application, these holes are all circular holes, wherein the lens hole, the light path hole H2, and the through hole H1 located in the optical path of the imaging light beam are coaxially arranged.

[0126] exist Figure 2 、 Figure 3 and Figure 5 In the illustrated embodiment, the filter holder 2221 is rotatably connected to the top side plate of the protective cover 260. The first driver 2222 is fixedly mounted on the side of the top side plate of the protective cover 260 in the Y direction, away from the excitation source module 210. The first driver 2222 drives the filter holder 2221 to rotate relative to the top side plate of the protective cover 260 via a synchronous belt. Furthermore, a cover 280 is provided on the side wall of the protective cover 260 to accommodate a driving pulley fixedly mounted on the output end of the first driver 2222. Thus, the entire synchronous belt drive mechanism is protected by the protective cover 260 and the cover 280, ensuring reliable rotation.

[0127] In a further optional embodiment, the optical engine module 200 further includes a guide base 270, which is detachably connected to the peripheral side plate of the protective cover 260. The guide base 270 is located between the excitation source module 210 and the protective cover 260. The guide base 270 is provided with a guide groove C and an excitation beam through hole H3, and the excitation beam through hole H3 is located in the guide groove C.

[0128] The guide groove C accommodates the light emitting end of each light emitting unit 212 in the excitation source module 210. The light source switching mechanism 211 in the excitation source module 210 can drive each light emitting unit 212 to move along the guide groove C, and allow the light emitting end of any light emitting unit 212 to align with the excitation beam through hole H3. The excitation beam through hole H3 is used to guide the excitation beam to the optical path splitting module 230.

[0129] In this embodiment, the guide seat 270 is used to cooperate with the excitation source module 210. The guide seat 270 is fixedly mounted on the peripheral side plate of the protective cover 260 and is provided with a guide groove C and an excitation beam through hole H3. The guide seat 270 cooperates with the excitation source module 210 to realize switching of the excitation beam.

[0130] Specifically, the light source switching mechanism 211 in the excitation source module 210 drives each light emitting unit 212 to change the position of each light emitting unit 212 in the guide groove C, so as to select the light emitting end of any light emitting unit 212 to align with the excitation beam through hole H3, thereby realizing switching of different excitation beams.

[0131] The extension direction of the guide groove C is the same as the movement direction of each light emitting unit 212. Therefore, each light emitting unit 212 can move along the guide groove C under the drive of the light source switching mechanism 211. In addition, the light source switching mechanism 211 can control the stop position, thereby aligning the light emitting end of one of the multiple light emitting units 212 with the excitation beam through hole H3. Accordingly, the excitation beam emitted by the light emitting unit 212 can pass through the excitation beam through hole H3 and be incident on the optical lens 231 in the optical path splitting module 230.

[0132] In addition, a second filter 2122 is fixedly mounted on the emitting end of the light emitting unit 212 , and the guide groove C can accommodate the second filter 2122 , thereby protecting the second filter 2122 .

[0133] In a specific application, the guide base 270 can be detachably connected to the protective cover 260 via a fastening member, such as a screw, a latch, etc. When the second optical filter 2122 needs to be maintained, or when the second optical filter 2122 of a different specification needs to be replaced, the guide base 270 is removed for replacement and maintenance.

[0134] It should be noted that a channel (not shown) needs to be provided on the peripheral side plate of the protective cover 260 to cooperate with the excitation beam through hole H3 to form a passage allowing the excitation beam to pass through, so that the excitation beam can be incident on the light path splitting module 230.

[0135] exist Figures 2 to 4 In the illustrated embodiment, the guide base 270 is disposed on a Y-direction side panel of the protective cover 260. The guide groove C extends along the X-direction, and the excitation beam through hole H3 is located in the middle of the guide groove C. There are two light emitting units 212 arranged side by side in the X-direction, and the light emitting end of each light emitting unit 212 is embedded in the guide groove C.

[0136] Furthermore, the light source switching mechanism 211 is a linear transmission module comprising a second drive member 2111, a support base 2112, a guide rail 2113, a slider 2114, and a screw drive unit 2115. The guide rail 2113 extends in the X direction and is fixedly mounted on the support base 2112. The slider 2114 is connected to the guide rail 2113 and the screw drive unit 2115. Each light emitting unit 212 is fixedly mounted on the slider 2114. The slider 2114 is slidably connected to the guide rail 2113. Driven by the second drive member 2111, the screw drive unit 2115 drives the slider 2114 along the guide rail 2113, thereby causing each light emitting unit 212 to move along the guide slot C.

[0137] It should be understood that when the second driving member 2111 stops driving, each light emitting unit 212 can stop moving. In this way, one light emitting unit 212 can be selected to stop at the excitation beam through hole H3 to align the light emitting end of the light emitting unit 212 with the excitation beam through hole H3.

[0138] It should be noted that the second driving member 2111 can be a stepping motor, a servo motor, etc. Of course, the light source switching mechanism 211 is not limited to a linear transmission module based on a screw drive, and can also be a linear transmission module based on a gear rack drive, etc.

[0139] In combination with the above, it can be seen that for the optical machine module 200, the various components of the optical machine module 200 are arranged based on the optical machine base plate 250, wherein the excitation source module 210, the optical path splitting module 230 and the white light source 240 are all directly fixedly mounted on the optical machine base plate 250, and the fluorescence imaging module 220 is integrated on the optical machine base plate 250 through the protective cover 260, which greatly improves the integration of the optical machine module 200 and reduces the space occupied by the fluorescence imaging device 1000.

[0140] Figure 6 for Figure 1 Schematic diagram of the detection platform 300. Figure 6In some optional embodiments, the detection platform 300 includes a multi-axis combined slide 310 and a sample supporting plate 320, wherein the sample supporting plate 320 is used to hold the sample carrier 400. The sample supporting plate 320 is disposed on the multi-axis combined slide 310, and the multi-axis combined slide 310 is used to adjust the position of the sample supporting plate 320 to change the position of the carrier 400.

[0141] In this embodiment, the multi-axis combined slide 310 provides multiple degrees of freedom of movement in various directions to adjust the position of the sample support plate 320. The sample support plate 320 can then drive the carrier 400 to change position. This allows the position of the carrier 400 within the optical path of the imaging beam to be changed, thereby obtaining clearer image data.

[0142] In practical applications, the sample support plate 320 is provided with a retaining groove D for accommodating the carrier 400. Specifically, the bottom end of the carrier 400 can be inserted into the retaining groove D to prevent the carrier 400 from slipping or falling. Furthermore, the multi-axis combined slide 310 is a three-axis combined slide, specifically providing translational degrees of freedom in the X, Y, and Z directions. The multi-axis combined slide 310 can be adjusted manually or electrically, depending on the application.

[0143] It should be noted that the limit slot D is not limited to Figure 6 The circular groove shown should be adaptively designed according to the structural shape of the carrier 400.

[0144] Figure 7 for Figure 1 Schematic diagram of the fluorescence imaging device 1000 from another perspective. Figure 8 for Figure 7 FIG. 1 is a schematic diagram of the fluorescence imaging device 1000 in the unfolded state. It should be noted that, Figure 8 The schematic diagram shown in FIG hides some of the sheet metal coverings of the frame 100. Figure 7 and Figure 8 The rack 100 is formed with a storage chamber R, which is used to integrate electronic and electrical control systems.

[0145] According to the above, the optical machine module 200 needs to introduce a light source and have the function of acquiring image data, and needs to automatically switch and accurately position each light emitting unit 212 and each first filter 223 to realize the function of switching the excitation light beam and the function of adapting the filter according to the imaging light beam excited by the excitation light beam. These functions all need to be automatically controlled through an electronic and electrical control system.

[0146] The electronic and electrical control system is housed in the storage room R and protected by the rack 100. Specifically, the electronic and electrical control system includes a power module 511, a white light source controller 512, an excitation light source controller 513, a driving device controller 514, and the like.

[0147] The power module 511 can convert industrial or household AC power into a DC control power supply, such as a 24V DC power supply or a 12V DC power supply. The white light source controller 512 is used to control the operation of the white light source 240. The excitation light source controller 513 can be used to control the specifications of the excitation light beam provided to the excitation source module 210. The drive device controller 514 is used to control the drive unit in the light source switching mechanism 211 and the first drive element 2222 and the second drive element 2111 in the filter switching mechanism 222.

[0148] exist Figure 7 In the illustrated embodiment, a power interface 515 and a communication interface 516 are further provided on one side of the rack 100 in the X direction. The power interface 515 is used to connect a power source to power the fluorescence imaging device 1000, and the communication interface 516 can be connected to a host computer, where the host computer refers to a personal computer (PC) or a console. It should be understood that the host computer can provide an operating interface that is user-friendly and interactive to implement functions such as interface control, data acquisition and analysis.

[0149] A cooling fan 517 is further provided on one side of the rack 100 in the Y direction. The cooling fan 517 can form convection between the storage chamber R and the outside world to reduce the temperature in the storage chamber R.

[0150] In addition, the optical machine base plate 250 is provided with a cable through hole H4, which is used for cables to pass through to connect to the optical machine module 200. The cables here include, for example, power supply lines, control lines, optical fibers, etc.

[0151] In a further embodiment, the rack 100 includes a movable mounting plate 110 , which is configured to be withdrawn from the storage chamber R.

[0152] In this embodiment, the movable installation plate 110 can integrate some electrical components, and these electrical components can be pulled out of the storage chamber R along with the movable installation plate 110 to facilitate inspection and maintenance.

[0153] exist Figure 8 In the illustrated embodiment, the power module 511 and the white light source controller 512 are both fixedly mounted on the movable mounting plate 110. Furthermore, a power interface 515 and a communication interface 516 are integrated on the outer side of the movable mounting plate 110. This is not limited to the illustrated embodiment, and the types of electrical components on the movable mounting plate 110 can be adjusted as needed.

[0154] It can be seen that the electronic and electrical control system in the fluorescence imaging device 1000 is integrated in the storage room R. Except for the host computer, all components are integrated on the frame 100. The components adopt a modular layout, which is easy to install and debug. The overall layout of the machine is compact and reasonable, and it takes up little space.

[0155] It should be noted that the fluorescence imaging device provided in this application can be applied to plant gene editing. Specifically, the fluorescence imaging device is used in the later stages of plant gene editing, such as in the phenotyping process. It uses fluorescently labeled specific proteins or gene products to observe their localization in plant cells or tissues, providing intuitive information for studying gene function.

[0156] To facilitate understanding of this solution, the following will exemplarily illustrate how to obtain fluorescent image data of the plant embryo using the fluorescent imaging device 1000 disclosed in this application, taking the plant embryo tissue as the shooting object.

[0157] Specifically, the carrier 400 is a culture dish and can be loaded with a plurality of containers containing plant embryos, and these plant embryos are fluorescently labeled.

[0158] The fluorescence imaging device 1000 can cooperate with the host computer to control the light source and the image acquisition unit 221 to realize the position positioning and development status judgment of the plant embryo and collect the plant embryo development data.

[0159] The fluorescence imaging device 1000 has both a white light imaging mode and a fluorescence imaging mode. In the white light imaging mode, the host computer can issue instructions to control the white light source controller 512 to turn on the white light source 240 and to turn off the excitation light source controller 513. In the fluorescence imaging mode, the host computer can issue instructions to control the white light source controller 512 to turn off the white light source 240 and turn on the excitation light source controller 513, and control the light source switching mechanism 211 in the excitation source module 210 to select the desired light emitting unit 212 to emit an excitation light beam of the corresponding wavelength.

[0160] In the white light imaging mode, the filter fixture 2221 switches to the bright field hole H12, and white light is irradiated on the plant embryos in the carrier 400. After being reflected by the plant embryos, it passes through the optical lens 231 and is incident on the image acquisition unit 221 through the bright field hole H12 to realize white light imaging. Then the host computer records the position information of each container containing plant embryos in the carrier 400.

[0161] The white light imaging mode is switched to the fluorescence imaging mode, and the filter holder 2221 simultaneously switches the corresponding fluorescence hole H11 to be in the optical path of the imaging beam. The excitation beam emitted by the excitation source module 210 is reflected by the optical lens 231 and incident on the plant embryo in the carrier 400. The plant embryo is excited to fluoresce, and the excited fluorescence passes through the first filter 223 in the corresponding fluorescence hole H11 of the filter holder 2221. After the stray light is filtered by the first filter 223, it is acquired by the image acquisition unit 221 to realize fluorescence imaging. Then the host computer records and marks the position where each plant embryo container in the carrier 400 generates fluorescence to sort these plant embryos.

[0162] It should be noted that the above description only illustrates the application of the fluorescence imaging device 1000 in plant gene editing to facilitate a better understanding of the present application and should not be construed as limiting the present application. That is, the fluorescence imaging device 1000 is not limited to plant gene editing and can also be used in other fields, such as medical research and drug development.

[0163] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Those skilled in the art can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A fluorescence imaging device, characterized in that: The apparatus comprises a frame (100), an optical machine module (200) and a detection platform (300), wherein the optical machine module (200) and the detection platform (300) are both arranged on the frame (100), and the detection platform (300) is used to load a carrier (400) for holding samples; The optical machine module (200) is configured to select excitation light beams of different wavelengths to be incident on the sample in the carrier (400) to excite an imaging light beam, and to match and filter the imaging light beam according to the excitation light beam to form an image.

2. The fluorescence imaging device according to claim 1, characterized in that The optical machine module (200) comprises an excitation source module (210), a fluorescence imaging module (220) and an optical path splitting module (230); The fluorescence imaging module (220) and the light path splitting module (230) are arranged at intervals in the direction of the light path where the imaging light beam is located, and the excitation source module (210) is located on the peripheral side of the light path where the imaging light beam is located; The excitation source module (210) is configured to selectively emit the excitation light beams of different wavelengths, and the light path splitting module (230) is capable of reflecting the excitation light beams to be incident on the sample in the carrier (400); The fluorescence imaging module (220) is used to match and filter the imaging light beam incident via the light path splitting module (230) according to the excitation light beam to form an image.

3. The fluorescence imaging device according to claim 2, characterized in that The excitation source module (210) includes a light source switching mechanism (211) and at least two light emitting units (212); Each of the light emitting units (212) is connected to the light source switching mechanism (211), and the light source switching mechanism (211) is used to select any one of the plurality of light emitting units (212) to emit the excitation light beam.

4. The fluorescence imaging device according to claim 2, characterized in that The fluorescence imaging module (220) comprises an image acquisition unit (221), a filter switching mechanism (222), and a plurality of first filters (223); A plurality of the first filters (223) are all arranged on the filter switching mechanism (222), and the filter switching mechanism (222) can select any one of the first filters (223) to be located on the optical path of the imaging light beam; The image acquisition unit (221) is used to receive the filtered imaging light beam to form an image.

5. The fluorescence imaging device according to claim 4, characterized in that The optical filter switching mechanism (222) comprises an optical filter fixing member (2221), a first driving member (2222) and a positioning unit (2223); The filter fixing member (2221) is provided with a plurality of through holes (H1), the plurality of through holes (H1) including a plurality of fluorescent holes (H11) and at least one bright field hole (H12), and each of the first filters (223) is arranged in a corresponding fluorescent hole (H11); The first driving member (2222) is connected to the filter fixing member (2221) to drive the filter fixing member (2221) to move, and the positioning unit (2223) is used to determine the movement position of the filter fixing member (2221) and cooperate with the first driving member (2222) to select any one of the through holes (H1) to be located on the optical path of the imaging light beam.

6. The fluorescence imaging device according to claim 2, characterized in that The optical machine module (200) further includes a white light source (240), wherein the white light source (240) is located between the optical path splitting module (230) and the detection platform (300) in the direction of the optical path of the imaging light beam; The white light source (240) is used to emit white light toward the sample in the carrier (400).

7. The fluorescence imaging device according to claim 6, characterized in that The optical machine module (200) further includes an optical machine base plate (250), and the optical path splitting module (230) includes an optical lens (231); The optical machine base plate (250) is provided with an optical path hole (H2), the optical path hole (H2) is located on the optical path of the imaging light beam, and the optical path splitting module (230) and the white light source (240) are respectively arranged on opposite sides of the optical machine base plate (250); The white light source (240) is arranged around the light path hole (H2), and the projection of the optical lens (231) in the direction of the light path where the imaging light beam is located overlaps the light path hole (H2).

8. The fluorescence imaging device according to claim 7, characterized in that: The optical machine module (200) further includes a protective cover (260), wherein the protective cover (260) is arranged on the optical machine base plate (250) and covers the optical path splitting module (230); The excitation source module (210) is arranged on the optical machine base plate (250) and is located on the peripheral side of the protective cover (260); the fluorescence imaging module (220) is arranged on the top side plate of the protective cover (260); and the filter fixing component (2221) of the fluorescence imaging module (220) is located inside the protective cover (260).

9. The fluorescence imaging device according to claim 8, characterized in that The optical machine module (200) further comprises a guide seat (270), wherein the guide seat (270) is detachably connected to the peripheral side plate of the protective cover (260); The guide seat (270) is located between the excitation source module (210) and the protective cover (260), and the guide seat (213) is provided with a guide groove (C) and an excitation beam through hole (H3), and the excitation beam through hole (H3) is located in the guide groove (C); The guide groove (C) accommodates the light emitting end of each light emitting unit (212) in the excitation source module (210); the light source switching mechanism (211) in the excitation source module (210) can drive the light emitting unit (212) to move along the guide groove (C) and align the light emitting end of any light emitting unit (212) with the excitation light beam through hole (H3); the excitation light beam through hole (H3) is used to guide the excitation light beam to the light path splitting module (230).

10. The fluorescence imaging device according to claim 1, characterized in that: The detection platform (300) includes a multi-axis combined slide (310) and a sample supporting plate (320), and the sample supporting plate (320) is used to hold the sample carrier (400); The sample supporting plate (320) is arranged on the multi-axis combined slide (310), and the multi-axis combined slide (310) is used to adjust the position of the sample supporting plate (320) to change the position of the carrier (400).

11. The fluorescence imaging device according to any one of claims 1 to 10, characterized in that: The fluorescence imaging device (1000) is used in plant gene editing.

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