Longan meat steamed American ginseng processed product for regulating blood sugar and preparation method and application thereof

Abstract

The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a longan aril steamed American ginseng processed product for regulating blood sugar, a preparation method and application thereof. The longan aril steamed American ginseng processed product is prepared by taking American ginseng as a main raw material and longan aril as an auxiliary material. Experiments show that the longan aril significantly enhances the blood sugar lowering effect of American ginseng. Further, the processing parameters are comprehensively optimized, and the prepared processed product is mainly used for treating diabetes (abnormal blood sugar) and its accompanying symptoms (such as insomnia) with deficiency of both qi and yin and blood stasis, and the blood sugar regulating effect is significantly better than that of American ginseng steamed products and American ginseng processed products prepared by using other auxiliary materials (such as ginger steamed American ginseng processed product), and is suitable for treating type 2 diabetes and its accompanying symptoms (such as insomnia).

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a longan aril steamed American ginseng processed product for regulating blood sugar as well as a preparation method and application thereof. BACKGROUND

[0002] American ginseng is a widely used tonic'medicine-food homology' traditional Chinese medicine, which is cold in nature and tonifying, and can be used instead of ginseng for people who do not want to take the warm and tonifying ginseng. American ginseng has the effects of tonifying qi and nourishing yin, clearing heat and generating fluid, and can be used for the treatment and prevention of 'diabetes' caused by qi deficiency and yin deficiency. At present, American ginseng is mostly used in the form of slices or powder, and there are problems such as single product and insufficient research depth. In addition, American ginseng is a cold tonifying product, which can cause abdominal pain in people with cold and can cause fire not to be released and cause cold and heat in people with fire, so the application range is limited.

[0003] Longan aril is warm in nature and sweet in taste, and has the effects of benefiting heart and spleen, tonifying qi and blood, and calming the mind. Since ancient times, longan aril has been a tonic product. When longan aril is used together with American ginseng, the medicinal properties of the two can be adjusted, and the effects of tonifying qi and nourishing yin and nourishing blood and calming the mind can be achieved. According to the classics of traditional Chinese medicine, longan aril can be used together with American ginseng, such as'recently, some people dislike the cold nature, and longan aril is steamed with longan aril and used' recorded in 'Supplement to the Compendium of Materia Medica', and 'Yu Linggao' and 'Longan American Ginseng Drink' recorded in ancient Chinese diet therapy prescriptions. However, these products are more focused on improving symptoms such as deficiency of qi and blood, blood deficiency, forgetfulness and insomnia. At present, there is no report on the combination of American ginseng and longan aril for treating diabetes. SUMMARY

[0004] Therefore, the present application provides a longan aril steamed American ginseng processed product for regulating blood sugar as well as a preparation method and application thereof. The processed product has a significant effect of regulating blood sugar and good curative effect on type 2 diabetes and its accompanying positive (insomnia).

[0005] The present application provides a preparation method of the longan aril steamed American ginseng processed product, which comprises the following steps: after longan aril is squeezed to obtain longan aril juice, the longan aril juice is mixed with American ginseng, and then the mixture is subjected to dampening, steaming, and drying to obtain the longan aril steamed American ginseng processed product.

[0006] The mass fraction of longan aril and American ginseng is 20-40 parts of longan aril and 100-200 parts of American ginseng.

[0007] American ginseng is a cold tonifying product, and has the effects of tonifying qi and nourishing yin, clearing heat and generating fluid. Longan aril is warm in nature, and has the effects of benefiting heart and spleen and tonifying qi and blood. Longan aril alone does not have the effect of reducing blood sugar, and on the contrary, longan aril is easy to cause blood sugar to rise due to its high sugar content.

[0008] The present application takes American ginseng as the main raw material, and a small amount of longan meat as the auxiliary material to prepare a processed product. The experimental results show that the processed product prepared by the present application has a significant effect of regulating blood sugar, and the effect is obviously better than that of American ginseng processed product alone and other medicinal ingredients (such as ginger) and American ginseng processed product. It is shown that longan meat can significantly enhance the hypoglycemic effect of American ginseng, and the combination of the two has a prominent effect on regulating blood sugar and has a good curative effect on type 2 diabetes.

[0009] In some specific embodiments, the mass ratio of the longan meat and American ginseng is 20 parts of longan meat and 200 parts of American ginseng; and the longan meat is fresh longan meat.

[0010] In some specific embodiments, the mass ratio of the longan meat and American ginseng is 30 parts of longan meat and 100 parts of American ginseng; and the longan meat is fresh longan meat.

[0011] In some specific embodiments, the mass ratio of the longan meat and American ginseng is 30 parts of longan meat and 200 parts of American ginseng; and the longan meat is fresh longan meat.

[0012] In some specific embodiments, the mass ratio of the longan meat and American ginseng is 40 parts of longan meat and 200 parts of American ginseng; and the longan meat is fresh longan meat.

[0013] In the present application, the equivalent concentration of the longan meat juice is 0.125g-1g of fresh longan meat crude drug equivalent / mL, preferably 0.5g-1g of fresh longan meat crude drug equivalent / mL; and the equivalent concentration can be specifically 0.5g of fresh longan meat crude drug equivalent / mL, 0.625g of fresh longan meat crude drug equivalent / mL, 0.75g of fresh longan meat crude drug equivalent / mL, 0.875g of fresh longan meat crude drug equivalent / mL, or 1g of fresh longan meat crude drug equivalent / mL.

[0014] In the present application, the solid-liquid ratio of the American ginseng and the longan meat juice is 2-10g:1mL, and can be specifically 2g:1mL, 4g:1mL, 6g:1mL, 8g:1mL, or 10g:1mL.

[0015] In the present application, the temperature for steaming and moistening is 20℃-40℃, and can be specifically 20℃, 25℃, 30℃, 35℃, or 40℃. The time for steaming and moistening is 1h-5h, and can be specifically 1h, 2h, 3h, 4h, or 5h. In the present application, the step of stirring and mixing every 0.5h during the steaming and moistening is further included.

[0016] In the present application, the temperature of the steaming is 100-120 DEG C, preferably 100-110 DEG C, and specifically can be 100 DEG C, 105 DEG C or 110 DEG C. The time of the steaming is 1-5 hours, preferably 2-4 hours, and specifically can be 2 hours, 3 hours or 4 hours. In the present application, the steaming is water-separation high-pressure steaming, and the present application does not have special restrictions on the equipment for the steaming, which can be water-separation high-pressure steaming in a high-pressure steam sterilization pot, or other equipment that can achieve the above-mentioned steaming conditions.

[0017] In the present application, the temperature of the drying is 50-80 DEG C, and specifically can be 50 DEG C, 60 DEG C, 70 DEG C or 80 DEG C. The time of the drying is 24-48 hours, and specifically can be 24 hours, 30 hours, 36 hours, 42 hours or 48 hours.

[0018] In the present application, the longan meat is fresh longan meat or dried longan meat, and preferably dried longan meat. In the present application, the American ginseng is fresh American ginseng or dried American ginseng, and preferably fresh American ginseng. The present application does not have special restrictions on the specific production place and source of the longan and American ginseng, and ordinary planting or ordinary commercially available products can be used. In the specific embodiments of the present application, dried longan meat produced in Guangxi and American ginseng produced in Jilin Province are used.

[0019] In the specific embodiments of the present application, the American ginseng is fresh American ginseng slice with a thickness of 2-3 mm.

[0020] In the present application, when the longan meat is dried longan meat, the method further comprises a step of soaking and swelling the dried longan meat in water before juicing. In the present application, the time of the soaking and swelling is 2-6 hours, and specifically can be 2 hours, 3 hours, 4 hours, 5 hours or 6 hours.

[0021] The present application also provides American ginseng processed products prepared by the above-mentioned preparation method.

[0022] The present application also provides the use of the above-mentioned American ginseng processed products or preparations using the American ginseng processed products as raw materials in the preparation of products that help maintain a healthy level of blood sugar.

[0023] In the present application, the products are large health foods, including foods and health foods.

[0024] In the present application, the products that help maintain a healthy level of blood sugar include at least one of the following functions:

[0025] (1) preventing and improving diabetes or its accompanying symptoms;

[0026] (2) preventing and improving pancreatic injury;

[0027] (3) preventing and improving liver injury.

[0028] In the present application, the accompanying symptoms of diabetes include insomnia.

[0029] In the present application, the diabetes is type 2 diabetes.

[0030] The present application takes American ginseng as the main raw material, longan arillus as the auxiliary material, and prepares the longan arillus steamed American ginseng processed product by compounding the two according to a specific ratio and adopting a specific process. The prepared longan arillus steamed American ginseng processed product has a significant effect of regulating blood sugar, and the effect is obviously better than that of other longan arillus steamed American ginseng processed products, existing ginger steamed American ginseng processed products and American ginseng processed products without auxiliary materials. The optimal processing technology obtained by optimization of the present application can obtain the maximum total saponin content and maintain a large total polysaccharide content under lower energy consumption, and various types of saponins and polysaccharides synergistically play a role in stabilizing abnormal blood sugar, relieving insomnia, and obviously improving the pancreatic and liver tissue lesions of type 2 diabetes rats, and has special potential in the treatment and prevention of type 2 diabetes and its accompanying symptoms (such as insomnia). BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 Total saponin and total polysaccharide contents (%) for longan arillus juice equivalent concentration single factor experiment;

[0032] Figure 2 Total saponin and total polysaccharide contents (%) for steaming time single factor experiment;

[0033] Figure 3 Appearance property results of orthogonal experiment, wherein A~I are the results of experiment numbers 1~9 in table 2, and A1 and A2 are the results after steaming and drying, respectively;

[0034] Figure 4 Blood glucose change curve of each group;

[0035] Figure 5 HE staining results of pancreas of each group;

[0036] Figure 6 HE staining results of liver of each group. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0038] In this paper, the descriptions of “including”, “containing” and “having” include the closed technical solutions consisting of the listed features, and also include the open technical solutions containing the listed features.

[0039] As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0040] Herein, reference to ranges of values, such as those for parameters, are to be understood as being continuous within the value range, and to include the minimum and maximum values of the range, as well as every value between these minimum and maximum values. Further, where ranges are expressed as integers, every integer between the minimum and maximum values of the range is included. In addition, where multiple ranges are provided to describe a characteristic or property, the ranges can be combined. In other words, unless otherwise indicated, all ranges disclosed herein are to be understood as encompassing any and all sub-ranges subsumed therein.

[0041] Only some numerical ranges are specifically disclosed herein. However, any lower limit can be combined with any upper limit to form a range not explicitly recited; and any lower limit can be combined with other lower limits to form a range not explicitly recited, as can any upper limit with any other upper limit to form a range not explicitly recited. In addition, each individual disclosed point or single numerical value can itself be combined as a lower limit or upper limit with any other point or single numerical value or with other lower limits or upper limits to form a range not explicitly recited.

[0042] The present application provides a processed American ginseng product, which comprises, by weight, 4-35 parts of dried longan meat and 200-1000 parts of American ginseng.

[0043] The processed American ginseng product and the processing method thereof are proposed based on the guidance of traditional Chinese medicine theory and the screening of modern scientific and technological research. American ginseng is a cold-nourishing product, which has the effects of tonifying qi and nourishing yin and clearing heat and generating fluid, and can be used for the treatment and prevention of "diabetes" caused by qi deficiency and yin deficiency. Longan meat is warm in nature, and has the effects of benefiting heart and spleen, tonifying qi and blood, and calming the mind. According to the Compendium of Materia Medica, longan meat is steamed for several times on a rice pot before use, or longan meat is steamed together with longan meat. The present application uses American ginseng as the main raw material and longan meat as the auxiliary material to prepare a longan meat steamed American ginseng product, and optimizes the process parameters by taking appearance, total saponins and total polysaccharides as the main indicators. The optimal processing technology obtained by optimization can obtain the maximum total saponin content under lower energy consumption, and maintain a larger total polysaccharide content. Various types of saponins and polysaccharides synergistically play a role in stabilizing abnormal blood glucose, relieving insomnia, and significantly improving the pathological changes of the pancreas and liver tissue of type 2 diabetic rats. It has special potential in the treatment and prevention of type 2 diabetes and its accompanying symptoms. In summary, the invention of longan meat steamed American ginseng product can expand the large health product series of American ginseng, improve the scientific and technological content and quality of American ginseng varieties, and ultimately increase the application range of American ginseng in medical care and enhance the market competitiveness of American ginseng.

[0044] The application further provides a preparation method of the American ginseng processed product, comprising the following steps: juicing longan arils, mixing the longan aril juice with American ginseng, and obtaining the American ginseng processed product through dampening, steaming and drying.

[0045] In specific embodiments of the application, the preparation process of the longan aril steamed American ginseng processed product specifically comprises the following steps:

[0046] (1) fresh American ginseng is taken, the surface is washed with clean water to remove the fine roots, and the fresh American ginseng slices with a thickness of 2-3 mm are obtained;

[0047] (2) longan arils are taken, soaked in water for 4 hours, and then repeatedly juiced, and the juice and the soaking residue are combined and filtered to obtain longan aril juice, wherein the longan arils are preferably dried longan arils, and the equivalent concentration of the longan aril juice is 0.125-1 g of fresh longan aril crude drug equivalent / mL;

[0048] (3) the fresh American ginseng slices in step (1) are mixed with the longan aril juice in step (2) at a ratio of 5 g:1 mL, and then dampened for 1-5 hours at a temperature of 23±2℃ (room temperature);

[0049] (4) the fresh American ginseng slices dampened by the longan aril juice in step (3) are placed in a sealed container and steamed in a high-pressure steam sterilization pot, wherein the steaming temperature is preferably 100-110℃, and the steaming time is preferably 2-4 hours;

[0050] (5) the fresh American ginseng slices steamed in a high-pressure steam sterilization pot in step (4) are dried by an electric heating air dryer to obtain the longan aril steamed American ginseng processed product.

[0051] Preferably, the drying temperature is 70℃, and the drying time is 24 hours.

[0052] In the application, there is no special limitation on the source of all raw materials, which can be purchased in the market.

[0053] Different processing parameters have an important influence on the blood glucose regulation effect of American ginseng processed products. The application provides a processing technology with the best hypoglycemic effect, which comprises the following steps: the ratio of fresh American ginseng slices to longan aril juice is 5 g:1 mL, the equivalent concentration of the longan aril juice is 0.75 g of fresh longan aril crude drug equivalent / mL, the dampening temperature is 20-40℃, the dampening time is 3 hours, the steaming temperature is 100℃, the steaming time is 2 hours, the drying temperature is 70℃, and the drying time is 24 hours. Experiments show that the American ginseng processed product prepared by the technology has the best effect on type 2 diabetes, which is significantly better than other combinations of process parameters.

[0054] In order to further illustrate the application, the innovative traditional Chinese medicine processed product with blood glucose regulation effect, the preparation method and application thereof are described in detail in combination with the embodiments.

[0055] The reagents and test reagents used in the following examples are all commercially available products.

[0056] Example 1

[0057] Single-factor experiment on the equivalent concentration of longan pulp juice: Based on the preparation method of steamed American ginseng products with longan pulp provided by this invention, the soaking time was fixed at 1 hour, the steaming temperature at 100℃, and the steaming time at 3 hours. The equivalent concentration of longan pulp juice was changed to five levels: 0.125 g / mL, 0.25 g / mL, 0.5 g / mL, 0.75 g / mL, and 1 g / mL. Steamed American ginseng products with longan pulp and juice of different concentrations were obtained. The total saponin content and total polysaccharide content of each product were determined by vanillin-perchloric acid method and phenol-sulfuric acid method, respectively. The results are as follows. Figure 1 As shown.

[0058] from Figure 1 It was found that the total saponin and total polysaccharide content was highest when the longan pulp juice equivalent concentration was 1 g / mL. Therefore, 1 g / mL was selected as the longan pulp juice equivalent concentration for subsequent single-factor experiments. Furthermore, the total saponin and total polysaccharide content was optimal at longan pulp juice equivalent concentrations of 1 g / mL, 0.75 g / mL, and 0.5 g / mL. Therefore, these three levels (1 g / mL, 0.75 g / mL, and 0.5 g / mL) were selected for subsequent orthogonal experiments.

[0059] Example 2

[0060] Single-factor experiment on soaking time: Based on the preparation method of steamed American ginseng with longan pulp provided by this invention and the results of Example 1, the equivalent concentration of longan pulp juice was fixed at 1 g / mL, the steaming temperature was 100℃, and the steaming time was 3 h. The soaking time was changed to five levels: 1 h, 2 h, 3 h, 4 h, and 5 h. Steamed American ginseng with longan pulp after soaking for different durations was obtained. The total saponin content and total polysaccharide content of each product were determined by vanillin-perchloric acid method and phenol-sulfuric acid method, respectively. The results are as follows. Figure 2 As shown.

[0061] from Figure 2 It was found that the total saponin content was highest after 4 hours of soaking, and the total polysaccharide content was highest after 2 hours of soaking. The combined total saponin and total polysaccharide content was the highest after 2 hours of soaking, therefore the optimal soaking time was 2 hours. In addition, the total saponin and total polysaccharide content of steamed longan pulp with American ginseng was optimal after 2 hours, 3 hours, and 4 hours of soaking. Therefore, three levels (2 hours, 3 hours, and 4 hours) were selected for subsequent orthogonal experiments.

[0062] Example 3

[0063] Based on the single factor experiment, the preferred level results obtained factor level table (such as table 1), further through SPSS27 software generates including longan juice equivalent concentration, steaming time, steaming temperature, steaming time four factors and three levels orthogonal test scheme list (such as table 2), according to the list respectively carried out 9 times experiment, and the appearance of each experiment processed product evaluation (such as table 3 and Figure 3 ), respectively with vanillin-perchloric acid method, phenol-sulfuric acid method for determination of total saponins, total polysaccharide content determination, with comprehensive score as the only index to each factor level for direct analysis of the primary and secondary order of the influence of each factor on the comprehensive score and the best factor level combination (such as table 4), using analysis of variance to evaluate the significance of the influence of each factor on the comprehensive score (such as table 5), finally the best processing technology. Among them, the comprehensive score rules are as follows:

[0064] The comprehensive score of processed products Y=Y1 / 3+Y2 / 3+Y3 / 3, wherein Y1=total saponin content (%) score, Y2=total polysaccharide content (%) score, Y3=appearance score, respectively, the highest value is set to 100 points, and the rest of the values / highest value*100 is the score obtained. Appearance score standard: no crack after steaming, special aroma, sweet and astringent, sweet and sweet taste of longan meat (5 points). After drying, mainly round or round pieces (5 points), red-brown, semi-transparent (10 points), complete, hard and brittle (10 points).

[0065] Table 1 orthogonal test factor level table

[0066]

[0067] Table 2 orthogonal test scheme list

[0068]

[0069] Table 3 orthogonal test appearance score table

[0070]

[0071] Table 4 orthogonal test direct analysis table

[0072]

[0073] Table 5 orthogonal test analysis of variance table

[0074]

[0075] From Table 4, it can be seen that the primary and secondary order of influence of the juice equivalent concentration of longan pulp, soaking time, steaming temperature and steaming time on the comprehensive score of the processed product is C (steaming temperature) > D (steaming time) > B (soaking time) > A (solid-liquid ratio), and the optimal factor level combination is A2B2C1D1, that is, the juice equivalent concentration of longan pulp is 0.75 g / mL; the soaking time is 3 h; the steaming temperature is 100℃; and the steaming time is 2 h. In addition, from Table 5, it can be seen that each factor has a significant influence (P < 0.05) on the comprehensive score of longan pulp steamed American ginseng.

[0076] Example 4:

[0077] Process verification experiment: based on the preparation method of longan pulp steamed American ginseng provided in the application and the orthogonal test optimization result, the juice equivalent concentration of longan pulp is determined to be 0.75 g / mL, the soaking time is 3 h, and the steaming condition is high-pressure water-sealed steaming at 100℃ for 2 h, and three longan pulp steamed American ginseng preparation experiments (L-1~L-3) are carried out, wherein in each preparation experiment, the raw materials are: longan pulp 30 g, fresh American ginseng 200 g, and the total saponin and total polysaccharide contents of each processed product are determined.

[0078] Table 6. Total saponin and total polysaccharide contents of verification experiments

[0079]

[0080] From Table 6, it can be seen that the total saponin content of longan pulp steamed American ginseng in three parallel experiments is higher than the maximum value in the orthogonal test, and the total polysaccharide content is close to the maximum value in the orthogonal test.

[0081] In summary, the optimal process condition can increase the maximum total saponin content of the processed product while maintaining the maximum total polysaccharide content of the processed product, and therefore the optimal processing technology of longan pulp steamed American ginseng is determined to be: the solid-liquid ratio of American ginseng and longan pulp juice is 5 g:1 mL, the juice equivalent concentration of longan pulp is 0.75 g / mL, the soaking temperature is 20~40℃, the soaking time is 3 h, the steaming temperature is 100℃, the steaming time is 2 h, the drying temperature is 70℃, and the drying time is 24 h.

[0082] Longan pulp steamed American ginseng processed products are prepared by using the optimal processing technology based on Example 4, and unsteamed dry American ginseng processed products, steamed American ginseng processed products and ginger steamed American ginseng processed products are used as controls to investigate the effect of longan pulp steamed American ginseng processed products on the treatment of type 2 diabetes.

[0083] Example 5:

[0084] I. Experimental animals and grouping

[0085] 1. Experimental animals:

[0086] SD male rats, weighing 200±20g, were provided by Liaoning Changsheng Biotechnology Co., Ltd.

[0087] Experimental animal license number SCXK (Liao) 2020-0001.

[0088] 2. Experimental drugs:

[0089] The prepared processed products of Example 4, steamed American ginseng processed products without additives (without ginger and without longan meat), and ginger steamed American ginseng processed products were ground through a No. 7 sieve, and each was fully suspended with 0.05% sodium carboxymethyl cellulose, with a drug concentration of 200 mg / mL.

[0090] The preparation of steamed American ginseng processed products involves steaming, drying process, etc. First, the optimal preparation process is screened: steaming at 100℃ for 3h, and the rest of the conditions are consistent with the optimal process conditions of Example 4. Then, steamed American ginseng processed products without additives are prepared using the optimal process.

[0091] Ginger steamed American ginseng processed products involve processes such as soaking, steaming, and drying after the addition of ginger. First, the optimal preparation process is screened: the crude drug concentration of fresh ginger is 0.5g / mL, and the soaking time is 4h, and the rest of the process is consistent with the optimal process conditions of Example 4. Then, ginger steamed American ginseng processed products are prepared according to the optimal process.

[0092] Among them, the American ginseng used in the above three processed products is the same batch.

[0093] 3. Experimental grouping and modeling:

[0094] 2. Type 2 diabetes model establishment: 48 rats were randomly divided into 8 healthy control group and 40 T2DM model group. After 4 weeks of high-sugar high-fat diet, the rats were fasted for 12h overnight, and STZ solution was prepared in 0.1mmol / L citrate buffer (PH=4.5) on ice bath, and immediately injected intraperitoneally at a dose of 35mg / kg to establish T2DM rat model. After two weeks of STZ administration, the rats were fasted for 12h, and the blood glucose concentration of the rats was determined by tail vein blood sampling. The fasting blood glucose value ≥16.7mmol / L was the standard for establishing T2DM model. The healthy control group rats were fed with regular diet throughout the experiment, and were given intraperitoneal injection of citrate buffer solution at the same conversion dose.

[0095] 4. Experimental administration:

[0096] The model group was randomly divided into a model group and an administration group of dried American ginseng, steamed American ginseng, ginger-steamed American ginseng, and longan-steamed American ginseng, with 8 rats in each group. The difference in blood glucose between the groups was <0.1 mmol / L. The model group and the control group were given the same dose of the corresponding drug suspension by gavage. The control group was given the corresponding suspension by gavage once a day. During the period, the appearance, sleep behavior, urination and defecation of each group of rats were observed, and blood glucose was monitored weekly for 8 consecutive weeks. All rats were sacrificed and the pancreas and liver were used for pathological examination.

[0097] II. Detection Indicators

[0098] Pancreas and liver were fixed with 4% formaldehyde fixative, dehydrated with a series of ethanol solutions, cleared with xylene, embedded in paraffin, and serially sectioned coronally using a Leica automated microtome to a thickness of 2 μm. The sections were mounted on pre-treated slides to prevent slide detachment, baked at 60°C for 40 min, and then stained with hematoxylin and eosin (HE) to observe the state of tissue damage.

[0099] III. Experimental Results

[0100] 1. Observation Results

[0101] In the healthy group, rats gained weight slowly, ate at night, and spent most of the day resting or curled up in a sleep state. They responded promptly to sudden sound and light stimuli, had a good appetite and exploratory behavior, had smooth and shiny fur, firm and smooth feces, and pale yellow urine. In the model group, rats experienced polydipsia, polyphagia, and polyuria, with some experiencing weight loss instead of gain. They also had significantly reduced sleep time, were restless, had no obvious rhythm between activity and sleep, had dull eyes, dull fur, sluggish reactions, soft or unformed feces, and yellow urine. After treatment with dried American ginseng, steamed American ginseng, and ginger-steamed American ginseng, the polydipsia, polyphagia, and polyuria were alleviated to varying degrees, and the rats gained weight slightly. However, daytime sleep time remained short and mostly fragmented light sleep. The rats had dull fur, sluggish reactions, and formed feces. After treatment with longan-steamed American ginseng, the polydipsia, polyphagia, and polyuria were alleviated, the rats gained weight, and daytime sleep time increased and became more rhythmic compared to the model group. The rats regained some shine in their fur, were emotionally stable, responded more promptly, and had formed feces. This indicates that type 2 diabetic rats have insomnia symptoms, and that fresh longan pulp and American ginseng not only improve the diabetic symptoms in rats, but also partially reverse the accompanying insomnia symptoms.

[0102] 2. Blood glucose monitoring results

[0103] like Figure 4 As shown, the blood glucose levels of rats in the type 2 diabetes model group remained high. After treatment with dried American ginseng, steamed American ginseng, and ginger-steamed American ginseng, the blood glucose levels fluctuated but decreased overall. After treatment with longan-steamed American ginseng, the blood glucose level decreased more significantly and the overall decrease was more stable. Its hypoglycemic effect showed an advantage over other American ginseng products starting from the fourth week, indicating that the blood glucose regulation effect of the longan-steamed American ginseng product of this invention is better and more stable.

[0104] 3. Results of HE staining of pancreas

[0105] like Figure 5 As shown, in the healthy group rats, islets of Langerhans were clearly visible in the pancreatic tissue, with full exocrine acini, large islets with regular shapes, numerous and regularly shaped cells, neatly arranged, full cytoplasm, and oval, centrally located nuclei. In the model group rats, the islets of Langerhans in the pancreas were atrophied, fibrotic, and irregularly shaped. The number of cells in the islets was reduced, the cytoplasm was sparse, and the nuclei were pyknotted, deeply stained, and clumped together. Treatment with dried American ginseng, steamed American ginseng, and ginger-steamed American ginseng products showed varying degrees of improvement, with an overall increase in the number of islets, but the area was relatively small and the shape was irregular. The number of cells in the islets increased, but the arrangement was loose. After treatment with longan-steamed American ginseng products, the number of islets increased, the area of ​​the islets increased, the shape of the islets became more regular, and the number of cells in the islets was abundant. In summary, pancreatic tissue in T2DM model rats showed obvious lesions, and processed American ginseng products had a certain therapeutic effect on them. Among them, the processed American ginseng product made from fresh longan pulp of this invention had a significantly better restorative effect on pancreatic tissue in diabetic models than processed American ginseng products made by other processes, and had a better therapeutic effect on diabetes.

[0106] 4. Liver HE staining results

[0107] like Figure 6 As shown, in the healthy group rats, the liver lobules were not clearly segmented, the hepatic cords were relatively neatly arranged, the hepatocytes were arranged radially around the central vein, the central vein endothelium was intact, and the hepatic sinusoidal structure, portal vascular and bile duct structures were relatively intact. No obvious degeneration, necrosis, congestion, dilation, inflammatory infiltration, fibrosis, or inflammatory cell infiltration was observed. In the model group rats, the liver lobule structure was severely blurred, the central vein was congested, the hepatic cords were disordered, the hepatocytes were swollen and significantly enlarged, and there was hypopigmentation and inflammatory cell infiltration. At the same time, nuclear staining was lightened or disappeared. After treatment with dried American ginseng, steamed American ginseng, and ginger-steamed American ginseng, some hepatocyte cord structures recovered and cell swelling was reduced, but varying degrees of fatty infiltration were observed in the central vein. After treatment with longan pulp and fresh American ginseng, the liver lobule structure was slightly blurred, most of the hepatic cord structures recovered, the hepatocytes were arranged more neatly, the hepatocyte swelling was reduced and the volume was significantly reduced, and the inflammatory infiltration was reduced. In summary, the liver tissue of T2DM rats showed obvious lesions, and the above-mentioned processed American ginseng products all had a certain ameliorative effect on these lesions. However, the recovery effect of fresh American ginseng with longan pulp on liver tissue damage in the diabetic model was significantly better than that of American ginseng products prepared by other processes, and no fatty infiltration occurred. This confirms that it has the effect of nourishing the spirit and regulating emotions, and provides a certain material basis for regulating type 2 diabetes accompanied by yin deficiency and insomnia.

[0108] The application has been fully and effectively achieved the purpose of the application through the above examples. Those skilled in the art should understand that the application includes but is not limited to the contents described in the drawings, tables and examples. Any modification that does not deviate from the function and principle of the application will be included in the scope of the claims.

Claims

1. A method for preparing a Longan steamed American ginseng processed product for helping to maintain a healthy level of blood sugar, characterized in that, The dried longan pulp and American ginseng are mixed, and then soaked, steamed and dried to obtain the longan pulp steamed American ginseng processed product. The mass ratio of the longan pulp to the American ginseng is 20-40 parts of longan pulp to 100-200 parts of American ginseng, and the longan pulp is measured by mass parts of fresh longan pulp. The longan pulp juice has an equivalent concentration of 0.5-1 g of fresh longan pulp crude drug equivalent / mL. The soaking is performed at a temperature of 20-40°C for 2-4 hours. The steaming is performed at a temperature of 100-110°C for 2-4 hours. The ratio of the American ginseng to the longan pulp juice is (2-10) g:1 mL.

2. The production method according to claim 1, characterized by, The mass ratio of the longan pulp to the American ginseng is: 20 parts of longan pulp to 200 parts of American ginseng, or 30 parts of longan pulp to 100 parts of American ginseng, or 30 parts of longan pulp to 200 parts of American ginseng, or 40 parts of longan pulp to 200 parts of American ginseng. The soaking is performed at a temperature of 20-40°C for 3 hours.

3. The preparation method according to claim 1, characterized in that, The steaming is performed at a temperature of 100°C for 2 hours.

4. The method of claim 1, wherein, The drying is performed at a temperature of 70°C for 24 hours.

5. The preparation method of claim 1, wherein, The ratio of the American ginseng to the longan pulp juice is 5 g:1 mL. The longan pulp juice has an equivalent concentration of 0.75 g of fresh longan pulp crude drug equivalent / mL. The soaking is performed at a temperature of 20-40°C for 3 hours. The steaming is performed at a temperature of 100°C for 2 hours. The drying is performed at a temperature of 70°C for 24 hours.

6. The method of any one of claims 1 to 5, wherein the method further comprises the step of: The longan pulp is fresh longan pulp or dried longan pulp, and the American ginseng is fresh American ginseng or dried American ginseng.

7. The preparation method according to claim 6, characterized in that, When the longan pulp is dried longan pulp, the method further comprises a step of soaking the dried longan pulp in water for swelling before the juicing, and the swelling is performed for 2-6 hours.

8. The longan pulp steamed American ginseng processed product prepared by the preparation method of any one of claims 1-7.

9. The use of Longan steamed American ginseng processed product of claim 8 or Longan steamed American ginseng processed product prepared by the preparation method of any one of claims 1-7 in the preparation of a product that helps maintain a healthy level of blood sugar; characterized in that, The product is a health food.

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