Manufacturing method of micro-channel detection sheet, micro-channel detection sheet and detection device

Abstract

The invention discloses a manufacturing method of a micro-channel detection sheet, the micro-channel detection sheet and a detection device. The method comprises the following steps: processing a first blind hole and a first micro-channel which are communicated with each other on a first light guide sheet; processing and forming a first through hole corresponding to the first blind hole in a second light guide sheet; a first waterproof ring is placed between the first through hole and the first blind hole; and overlapping the second light guide sheet on the first light guide sheet for bonding to form the micro-channel detection sheet with the first waterproof ring embedded therein. The detection device is provided with a micro-channel detection sheet and a fluid leading-in element, the fluid leading-in element comprises a plate body and a first injection structure arranged on the plate body, and the first injection structure is provided with a first liquid injection piece protruding out of the surface of one side of the plate body and used for being inserted into the first through hole so as to inject liquid into the first micro-channel. Therefore, rapid and stable joint of the injection opening is achieved, and the liquid leakage risk is effectively reduced.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic detection products, and in particular to a method for manufacturing a microfluidic detection sheet, a microfluidic detection sheet, and a detection device. Background Art

[0002] Microfluidic detection technology is an analytical method used in microfluidic devices. By designing a microfluidic structure on the test piece, the flow path and flow rate of trace liquid samples in the channel are controlled, thereby realizing functions such as sample separation, reaction, mixing or detection. This type of technology is commonly used in fields such as biomedical testing, environmental analysis, chemical reaction monitoring and food safety testing. It has many advantages such as small sample usage, short reaction time and high degree of operation automation. In actual applications, the detection process usually includes the introduction of sample liquid, reaction in a specific detection area after passing through the microchannel, and finally obtaining an analytical result that can be interpreted. In order to accurately introduce the liquid into the corresponding microchannel of the test piece, the current practice adopted by the industry is to use a liquid guide tube with a plastic screw mechanism to lock it to the injection port, so that the liquid can flow from the liquid guide tube into the corresponding channel.

[0003] However, this method of locking the catheter to the injection port one by one with plastic screws has many disadvantages. First, when there are multiple injection ports on the test piece, the locking operation must be repeated multiple times, which is not only time-consuming but also prone to installation errors, increasing the burden on the operator. Second, the tightness between the plastic screw and the test piece may produce errors due to different assembly forces or material deformation, resulting in an increased risk of liquid leakage, thereby affecting the accuracy of the test results. In addition, repeated disassembly and assembly may also cause damage to the test piece, reducing its possibility of reuse. Therefore, how to simplify the installation procedure of the injection structure, improve the stability of liquid introduction and avoid leakage has become a practical issue that needs to be overcome in microfluidic detection technology.

[0004] In view of this, the inventors summarized their rich experience in related professional fields, conceived and proposed a manufacturing method of a microfluidic detection sheet, a microfluidic detection sheet and a detection device to improve the defects in the installation and sealing of the liquid injection structure in the existing technology, thereby improving the overall detection efficiency and accuracy. Summary of the Invention

[0005] One of the objectives of the present invention is to provide a method for manufacturing a microfluidic detection sheet, a microfluidic detection sheet, and a detection device, which can be made into a microfluidic detection sheet with high tightness and anti-leakage properties, and can be used with corresponding fluid introduction elements to achieve rapid plug-in and installation, connection, and stable liquid conduction, thereby improving the operational efficiency during microfluidic detection applications.

[0006] To achieve the above-mentioned objectives, the present invention, in one embodiment, proposes a method for manufacturing a microfluidic detection sheet, comprising the following steps: a first processing step: forming at least one first blind hole and at least one first microfluidic channel on a first light guide sheet, wherein the first blind hole and the first microfluidic channel are interconnected; a second processing step: forming at least one first through hole on a second light guide sheet, wherein the positions of the first through hole and the first blind hole correspond to each other; a placement step: placing a first waterproof ring between the first through hole and the first blind hole; and a bonding step: stacking the second light guide sheet on the first light guide sheet and bonding them, thereby forming a microfluidic detection sheet with the first waterproof ring embedded therein. The microfluidic detection sheet manufactured through this manufacturing process can effectively improve the sealing performance during liquid introduction, reduce the risk of liquid leakage, and ensure the fluid stability and detection accuracy of the microfluidic system.

[0007] Based on the aforementioned embodiment, another embodiment discloses that the first processing step further includes forming at least one second blind hole on the first light guide plate; the second processing step further includes forming at least one second through hole and at least one second microfluidic channel on the second light guide plate, wherein the second through hole and the second microfluidic channel are interconnected and the positions of the second through hole and the second blind hole correspond to each other; the placement step further includes placing a second waterproof ring between the second through hole and the second blind hole; and the microfluidic detection sheet formed in the bonding step is further embedded with the second waterproof ring. Thus, a microfluidic detection sheet suitable for multiple sets of quick plug-in and quick-disconnect injection applications is produced.

[0008] In addition, to facilitate the placement of the waterproof ring, another embodiment discloses that the first blind hole is slightly larger than the first through hole, and in the placement step, the first waterproof ring is placed in the first blind hole.

[0009] Based on the same technical concept, the present invention also proposes, in one embodiment, a microfluidic detection sheet, comprising: a first light guide sheet having at least one first blind hole and at least one first microfluidic channel, wherein the first blind hole and the first microfluidic channel are interconnected; a second light guide sheet stacked on the first light guide sheet and having at least one first through hole, wherein the positions of the first through hole and the first blind hole correspond to each other, wherein the first light guide sheet and the second light guide sheet are integrally formed by bonding; and at least one first waterproof ring embedded between the first through hole and the first blind hole. Thus, by forming an integrally formed and highly sealed sheet structure, the sealing and fluid stability during the injection process can be ensured, effectively preventing leakage. This structure also allows for quick plugging and unplugging, thereby enhancing the convenience of liquid injection.

[0010] Based on the foregoing embodiment, another embodiment is disclosed in which the microchannel detection sheet also includes at least one second waterproof ring, and the first light guide sheet also has at least one second blind hole, the second light guide sheet also has at least one second through hole and at least one second microchannel, the second through hole and the second microchannel are connected to each other, and the positions of the second through hole and the second blind hole correspond to each other; the second waterproof ring is buried between the second through hole and the second blind hole, thereby achieving the effect of multiple groups of plug-in and injection use.

[0011] In yet another embodiment, the first blind hole is slightly larger than the first through hole, so as to be more conducive to the installation and assembly stability of the first waterproof ring.

[0012] Based on the same technical concept, the present invention further proposes a detection device in one embodiment, comprising: a microfluidic detection plate, including a first light guide plate, a second light guide plate, and at least one first waterproof ring, wherein the first light guide plate has at least one first blind hole and at least one first microfluidic channel, and the first blind hole and the first microfluidic channel are interconnected; the second light guide plate is stacked on the first light guide plate and has at least one first through hole, the positions of the first through hole and the first blind hole correspond to each other, wherein the first light guide plate and the second light guide plate are integrated by bonding; the first waterproof ring is embedded between the first through hole and the first blind hole; and a fluid introduction element, including a plate body and at least one first injection structure, the first injection structure is provided on the plate body and has a first liquid injection part protruding from a side surface of the plate body, which is used to be inserted into the first through hole to inject liquid into the first microfluidic channel. In this way, rapid alignment and stable fixed plug-in installation can be achieved, greatly simplifying the operation process, while also having good liquid injection stability and leak-proof effect.

[0013] Similarly, based on the aforementioned embodiment, another embodiment discloses that the microfluidic detection sheet further includes at least one second waterproof ring, and the first light guide sheet further includes at least one second blind hole, the second light guide sheet further includes at least one second through hole and at least one second microfluidic channel, the second through hole and the second microfluidic channel are interconnected, and the positions of the second through hole and the second blind hole correspond to each other; the second waterproof ring is buried between the second through hole and the second blind hole; the fluid introduction element further includes at least one second injection structure, the second injection structure is provided on the plate body and has a second liquid injection part protruding from one side surface of the plate body, which is used to be inserted into the second through hole to inject liquid into the second microfluidic channel. This provides the function of multiple sets of quick plug-in and pull-out liquid injection applications.

[0014] In yet another embodiment, the first liquid injection member is a hollow tube structure with an inner diameter of 2±0.2 mm to improve the stability of liquid injection.

[0015] Taking into account the stability of the first waterproof ring, the first blind hole can be slightly larger than the first through hole, thereby enhancing the structural embedding strength of the first waterproof ring.

[0016] In summary, the manufacturing method of the microfluidic detection sheet, the microfluidic detection sheet and the detection device disclosed in the present invention form corresponding microfluidic channels and holes by processing the first and second light guide sheets, and place a waterproof ring between the two and then bond them, so that the structure can be formed in one piece, with both high sealing and structural stability, and thus the microfluidic detection sheet has a buried waterproof ring design, which can ensure that there will be no leakage when the liquid is introduced. The detection device uses a matching fluid introduction element to achieve fast and stable docking and liquid injection by plugging the injection piece into the through hole of the microfluidic detection sheet, eliminating complicated locking operations, and can effectively prevent leakage and improve detection operation efficiency. Furthermore, the present invention also proposes various detailed technical features that can be added to the aforementioned manufacturing method, microfluidic detection sheet and detection device, and the specific content and effects are described in the above paragraphs. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Flowchart of the manufacturing steps of a microfluidic detection chip according to one embodiment of the present invention.

[0018] Figure 2 Schematic diagram of the decomposition of the microfluidic detection chip structure according to one embodiment of the present invention.

[0019] Figure 3 Schematic diagram of the structure of a microfluidic detection chip according to one embodiment of the present invention.

[0020] Figure 4 Schematic diagram of the structure of a detection device according to one embodiment of the present invention.

[0021] Figure 5 This is a flow chart of the manufacturing steps of a microfluidic test chip according to another embodiment of the present invention.

[0022] Figure 6 This is a schematic diagram of the decomposition structure of a microfluidic detection chip according to another embodiment of the present invention.

[0023] Figure 7 Schematic diagram of the structure of a microfluidic detection chip according to another embodiment of the present invention.

[0024] Figure 8 This is a schematic structural diagram of a detection device according to another embodiment of the present invention. DETAILED DESCRIPTION

[0025] As mentioned above, in view of the problems faced by current microfluidic detection related devices such as difficulty in assembly and risk of liquid leakage, in order to effectively improve these defects, the inventors have conceived and proposed a new microfluidic detection sheet, its manufacturing method and related detection device to improve the efficiency of the detection operation, and have excellent structural sealing to effectively prevent liquid leakage. Below, the microfluidic detection sheet, manufacturing method and detection device are described in detail through drawings and text content. The structure, size, proportion, size, shape or application status shown in each figure is only for illustration, used to illustrate the technical features of the present invention, and does not represent the actual structural design, which is explained in advance.

[0026] Please see first Figure 1 and Figure 2 The following is a flowchart of the manufacturing steps of a microfluidic test sheet according to one embodiment of the present invention and a schematic diagram of the microfluidic test sheet structure. First, the manufacturing method of the microfluidic test sheet is described. The manufacturing method includes the following steps: a first processing step, a second processing step, a placement step, and a bonding step.

[0027] The first processing step is to form at least one first blind hole 10 and at least one first microchannel 11 on a first light guide plate 1, and the first blind hole 10 and the first microchannel 11 are connected to each other (step S11). The second processing step is to form at least one first through hole 20 on a second light guide plate 2, and the positions of the first through hole 20 and the first blind hole 10 correspond to each other (step S12). It should be pointed out in particular that although the above process is divided into the first processing step and the second processing step for the convenience of explanation, the description order of these steps should not be regarded as a restriction on the actual execution order. In actual application, the order can be adjusted according to the process requirements. For example, the second light guide plate 2 can be processed to form the first through hole 20 first, and then the first blind hole 10 and the first microchannel 11 can be processed on the first light guide plate 1, and the positions of the first blind hole 10 and the first through hole 20 can be made to correspond to each other. This still falls within the technical scope covered by the present invention. The placement step is to place a first waterproof ring 3 between the first through hole 20 and the first blind hole 10 (step S13), and the final bonding step is to stack the second light guide 2 on the first light guide 1 and bond it, thereby forming a microfluidic detection sheet 9 with the first waterproof ring 3 buried inside (step S14). Among them, the first light guide 1 and the second light guide 2 can be made of plastic sheets with light-transmitting properties, so that the manufactured microfluidic detection sheet 9 can allow light of various wavelengths to enter for related detection applications. For a schematic diagram of the overall structure of the microfluidic detection sheet 9, please refer to Figure 3 , which is a schematic diagram of the structure of a microfluidic detection sheet according to an embodiment of the present invention. In addition, in order to illustrate the structure of the first light guide sheet 1 and the second light guide sheet 2, Figure 3The boundary between the first light guide plate 1 and the second light guide plate 2 is retained around the perimeter of the microfluidic detection plate 9. However, in practice, due to the light-transmitting properties of the first and second light guide plates 1 and 2 and their high degree of adhesion achieved through bonding, the boundary around the microfluidic detection plate 9 is, in principle, almost invisible to the naked eye.

[0028] The process method utilizes bonding technology to integrate a first light guide plate 1 and a second light guide plate 2 into a single body, forming a highly adhesive microfluidic detection plate 9. Initially, the process considers the final detection plate's structural configuration and liquid guidance requirements, and corresponding structures, such as a first blind hole 10, a first microfluidic channel 11, and a first through hole 20, are machined into the first and second light guide plates 1 and 2, respectively. Furthermore, to effectively enhance the liquid-tightness of the microfluidic detection plate 9 and optimize the stability and efficiency of subsequent liquid injection operations, the process method employs a first waterproof ring 3 positioned between the first blind hole 10 and the first through hole 20. The double-layer structure allows the first waterproof ring 3 to be embedded within the surface of the first and second light guide plates 1 and 2 after bonding. It is worth noting that if the waterproof ring is simply placed on the surface of the detection plate at the injection hole location, its sealing effect is susceptible to operational errors or external interference, resulting in displacement or dislodging, making it difficult to maintain liquid-tightness over the long term. Therefore, the manufacturing method adopts the strategy of dividing the microfluidic detection plate 9 into two and then bonding them together. Through the structure of the first through hole 20 and the first blind hole 10, the first waterproof ring 3 can be stably located between the first light guide plate 1 and the second light guide plate 2 after bonding, thereby improving the sealing performance and structural stability. In addition, through the above steps, the first light guide plate 1 and the second light guide plate 2 can achieve a nearly seamless combination state, thereby effectively improving the mechanical strength and liquid sealing of the overall structure, and also helping to ensure the precise alignment of multiple structures (such as the first through hole 20, the first blind hole 10 and the first microfluidic channel 11), so that the first through hole 20, the first blind hole 10 and the first microfluidic channel 11 together constitute a structure for detection, making the liquid injection and liquid guiding process smoother and more controllable, providing a highly reliable foundation for subsequent detection operations.

[0029] In addition, considering the precise positioning and convenience requirements of the first waterproof ring 3, in one implementation, the first blind hole 10 can be slightly larger than the first through hole 20, and in the placement step, the first waterproof ring 3 is placed in the first blind hole 10. In this way, the first waterproof ring 3 can be restricted by the first blind hole 10, which helps it to be stably fixed in the predetermined position during the initial positioning stage, avoiding displacement or sliding during the assembly process. When the second light guide 2 is stacked and bonded, the first waterproof ring 3 is firmly clamped between the first blind hole 10 and the corresponding first through hole 20, thereby forming a structure buried inside the first light guide 1 and the second light guide 2. In other words, this design not only improves the positioning accuracy and installation convenience of the first waterproof ring 3, but also further enhances the overall liquid tightness and structural stability of the microchannel detection plate 9.

[0030] Please continue to see again Figure 2 and Figure 3 Based on the same technical concept, the following describes a microfluidic detection sheet of the present invention. The microfluidic detection sheet 9 includes a first light guide sheet 1, a second light guide sheet 2 and at least one first waterproof ring 3.

[0031] The first light guide plate 1 has at least one first blind hole 10 and at least one first microchannel 11, and the first blind hole 10 and the first microchannel 11 are interconnected. The second light guide plate 2 is stacked on the first light guide plate 1 and has at least one first through hole 20, which corresponds to the position of the first blind hole 10. The first and second light guide plates 1 and 2 are bonded together to form a single piece. The first waterproof ring 3 is embedded between the first through hole 20 and the first blind hole 10.

[0032] The microfluidic detection sheet 9 has high liquid tightness and high structural stability. By bonding the first light guide sheet 1 and the second light guide sheet 2 to form the two sheets of material into one, not only can the strength and stability of the overall structure be greatly improved, but also a nearly seamless high-tightness effect can be achieved. Furthermore, the corresponding design of the first blind hole 10 and the first through hole 20 of the microfluidic detection sheet 9 can form a through but controlled fluid guide channel between the second light guide sheet 2 located on the upper layer and the first light guide sheet 1 located on the lower layer, and cooperate with the first microchannel 11 formed inside the microfluidic detection sheet 9 to effectively guide the liquid to flow along a predetermined path. At the same time, the first waterproof ring 3 is buried inside the microfluidic detection sheet 9, which can effectively prevent the first waterproof ring 3 from falling off or shifting due to mechanical stress, repeated plugging and unplugging, or liquid pressure, thereby ensuring that the liquid will not leak to an unexpected location, strengthening the overall liquid tightness and long-term reliability, and facilitating the rapid plugging and unplugging of the subsequent supporting injection mechanism. In detection applications, the microfluidic detection sheet 9 facilitates the entry of light of various wavelengths, and can be used with an optical lens to capture and obtain liquid detection results.

[0033] In one embodiment, the first blind hole 10 can be slightly larger than the first through hole 20. By making the first blind hole 10 slightly larger than the first through hole 20, not only does it help improve the placement accuracy and processing tolerance of the first waterproof ring 3, but it also forms a stable interlocking structure, allowing the first waterproof ring 3 to maintain its embedded position after bonding the first light guide plate 1 and the second light guide plate 2, preventing displacement and falling off, thereby enhancing the overall liquid tightness and reliability of the microfluidic detection plate 9.

[0034] Please continue to match Figure 4 , which is a schematic diagram of the structure of the detection device of one embodiment of the present invention. Based on the technical foundation of the microfluidic detection sheet 9, the present invention further proposes a detection device 8, which includes a microfluidic detection sheet 9 and a fluid introduction element 80. The microfluidic detection sheet 9 is the same as the above, including a first light guide 1, a second light guide 2 and at least one first waterproof ring 3. The first light guide 1 has at least one first blind hole 10 and at least one first microfluidic channel 11, and the first blind hole 10 and the first microfluidic channel 11 are connected to each other. The second light guide 2 is stacked on the first light guide 1 and has at least one first through hole 20, and the positions of the first through hole 20 and the first blind hole 10 correspond to each other, wherein the first light guide 1 and the second light guide 2 are integrated by bonding. The first waterproof ring 3 is buried between the first through hole 20 and the first blind hole 10. The fluid introduction element 80 includes a plate 801 and at least one first injection structure 802. The first injection structure 802 is disposed on the plate 801 and includes a first injection member 8021 protruding from one side of the plate 801. The first injection member 8021 is inserted into the first through-hole 20 to inject liquid into the first microfluidic channel 11. In practical applications, the first injection structure 802 may, for example, be an assembly comprising a tube, a fixing member, and the first injection member 8021. The fixing member is disposed on one side of a reserved opening in the plate 801. The first injection member 8021 is disposed on the other side of the reserved opening and protrudes from the plate 801. The tube passes through the center of the fixing member and is fixed to the first injection member 8021, allowing liquid in the tube to be injected into the first microfluidic channel 11 through the first injection member 8021. The first injection structure 802 can be used to inject various liquids, such as test reagents and blood to be tested, into the microfluidic test strip to facilitate fluid testing, depending on the requirements and type of testing application.

[0035] Therefore, given the special structural design of the microfluidic detection sheet 9, the fluid introduction element 80 can be directly inserted into the first through hole 20 of the microfluidic detection sheet 9 through the first liquid injection piece 8021 protruding from the side of the plate 801, so that the liquid is introduced into the corresponding first microfluidic channel 11. The plug-in liquid injection structure design can achieve rapid alignment and stable fixation, greatly simplifying the operation process, reducing the probability of assembly errors, and improving overall operation efficiency. At the same time, after insertion, the first liquid injection piece 8021 forms a close contact with the microfluidic detection sheet 9, and has good liquid sealing and injection stability, which can effectively prevent liquid leakage or escape during the injection process, further ensuring the stable flow of microfluids in the detection sheet, improving detection accuracy, and having multiple advantages such as leak prevention, pollution prevention, and convenient operation.

[0036] Similarly, the design of making the first blind hole 10 slightly larger than the first through hole 20 is also applicable to the aforementioned detection device to improve the liquid tightness of the micro-channel detection sheet 9 .

[0037] Furthermore, in one embodiment, the first liquid injection member 8021 is a hollow tube with an inner diameter D1 of 2±0.2 mm. This dimensioning helps control the flow of liquid during the injection process, reduces the risk of liquid remaining on the tube wall due to adsorption, and improves the accuracy and flow rate stability of liquid injection.

[0038] Please see next Figure 5 and Figure 6 , which is a flowchart of the manufacturing steps of the microfluidic detection sheet and a schematic diagram of the decomposition of the structure of the microfluidic detection sheet according to another embodiment of the present invention. Disclosed herein is another embodiment of the method for manufacturing a microfluidic detection sheet. In this embodiment, the manufacturing method can produce a microfluidic detection sheet having multiple groups of holes to facilitate quick matching and plugging. Specifically, in the first processing step, in addition to forming the first blind hole 10 and the first microchannel 11 on the first light guide 1, at least one second blind hole 12 is also formed on the first light guide 1 (step S21). In the second processing step, in addition to forming the first through hole 20 on the second light guide 2, at least one second through hole 21 and at least one second microchannel 22 are also formed on the second light guide 2, and the second through hole 21 and the second microchannel 22 are connected to each other, and the positions of the second through hole 21 and the second blind hole 12 correspond to each other (step S22). In the placement step, in addition to placing the first waterproof ring 3 between the first through hole 20 and the first blind hole 10, a second waterproof ring 4 is also placed between the second through hole 21 and the second blind hole 12 (step S23). Finally, in the bonding step, the second light guide 2 is stacked on the first light guide 1 and bonded to form the microfluidic detection plate 9, which is embedded with the first waterproof ring 3 and the second waterproof ring 4 (step S24). For a schematic diagram of the overall structure of the microfluidic detection plate 9, please refer to Figure 7, which is a schematic diagram of the structure of a microfluidic detection sheet according to another embodiment of the present invention. Similarly, in order to facilitate the illustration of the structure of the first light guide sheet 1 and the second light guide sheet 2, Figure 7 The boundary between the first light guide plate 1 and the second light guide plate 2 is also retained on the peripheral side of the microfluidic detection plate 9. However, as in the previous embodiment, the boundary on the peripheral side of the microfluidic detection plate 9 is in principle almost invisible to the naked eye.

[0039] In short, the microfluidic detection sheet 9 made by the manufacturing method has a first group of microfluidic channel structures (first blind hole 10, first microfluidic channel 11, first through hole 20) configured on the first light guide 1, and a second group of microfluidic channel structures (second blind hole 12, second through hole 21, second microfluidic channel 22) configured on the second light guide 2, so that each group of microfluidic channel structures is layered along the Z-axis direction, which can avoid the channels from overlapping and causing structural interference, and can also reduce the chance of contamination during injection. In addition, the microfluidic detection sheet 9 is configured with multiple groups of microfluidic channels and injection holes at the same time, that is, it has multiple groups of plug-in functions, which is conducive to improving the density and efficiency of multi-channel detection. In other words, the microfluidic detection sheet 9 can thus have functions such as multi-point injection, multi-channel diversion or parallel detection of multiple samples. This configuration not only increases the number of detection channels and supports higher-throughput analysis operations, but also allows users to quickly select and plug in the corresponding injection components according to actual needs, thereby improving operational flexibility, injection efficiency and convenience.

[0040] Incidentally, in this embodiment, the first blind hole 10 can be made slightly larger than the first through hole 20, and the first waterproof ring 3 can be placed within the first blind hole 10 during the placement step. Alternatively, the first blind hole 10 can be made slightly larger than the first through hole 20, and the second blind hole 12 can be made slightly larger than the second through hole 21. In this case, the first waterproof ring 3 can be placed within the first blind hole 10, and the second waterproof ring 4 can be placed within the second blind hole 12. This helps to stably secure the first and second waterproof rings 3 and 4 in their intended positions, facilitating subsequent bonding operations and allowing the first and second waterproof rings 3 and 4 to be precisely positioned.

[0041] Please continue to match Figure 5 and Figure 6Next, the structural aspects of the microfluidic detection sheet will be described. In this embodiment, the microfluidic detection sheet 9 also includes a first light guide sheet 1, a second light guide sheet 2, and a first waterproof ring 3. However, unlike the previous embodiment, the first light guide sheet 1 further includes at least one second blind hole 12, and the second light guide sheet 2 further includes at least one second through hole 21 and at least one second microfluidic channel 22. The second through hole 21 and the second microfluidic channel 22 are interconnected, and the positions of the second through hole 21 and the second blind hole 12 correspond to each other. The microfluidic detection sheet 9 also includes at least one second waterproof ring 4, which is embedded between the second through hole 21 and the second blind hole 12.

[0042] In this way, the microfluidic detection chip 9, with its multiple sets of holes and channel structure characteristics, can be used in subsequent applications to achieve the effect of quick insertion and removal and improve detection throughput, thereby enhancing detection efficiency and convenience.

[0043] Similarly, the microchannel detection sheet 9 can further make the first blind hole 10 slightly larger than the first through hole 20 to improve the structural stability of the first waterproof ring 3; or simultaneously make the first blind hole 10 slightly larger than the first through hole 20 and the second blind hole 12 slightly larger than the second through hole 21, thereby simultaneously improving the structural stability of the first waterproof ring 3 and the second waterproof ring 4.

[0044] Please continue to match Figure 8 , which is a schematic diagram of the structure of a detection device according to another embodiment of the present invention. In this embodiment, the detection device 8 also includes a microfluidic detection sheet 9 and a fluid introduction element 80, and the microfluidic detection sheet 9 also includes at least one second waterproof ring 4, and the first light guide plate 1 also has at least one second blind hole 12, and the second light guide plate 2 also has at least one second through hole 21 and at least one second microfluidic channel 22, the second through hole 21 and the second microfluidic channel 22 are interconnected, the positions of the second through hole 21 and the second blind hole 12 correspond to each other, and the second waterproof ring 4 is buried between the second through hole 21 and the second blind hole 12. The fluid introduction element 80 also includes at least one second injection structure 803, which is provided on the plate body 801 and has a second liquid injection part 8031 ​​protruding from one side surface of the plate body 801, which is used to be inserted into the second through hole 21 to inject liquid into the second microfluidic channel 22.

[0045] Thus, the detection device 8 of this embodiment expands the functionality of the microfluidic detection chip 9, further enhancing its multi-channel liquid handling capabilities. Through the layered configuration and corresponding design of the structure, combined with the second liquid injection component 8031 ​​provided on the fluid introduction element 80, it enables rapid alignment and insertion of multiple liquid injection structures, as well as liquid introduction. This combination of multiple channels and corresponding liquid injection components not only facilitates high-throughput detection operations but also significantly simplifies the operational process.

[0046] Based on the detection device 8 of this embodiment, the structural features of making the first blind hole 10 slightly larger than the first through hole 20 and making the first liquid injection member 8021 a hollow tube with an inner diameter D1 of 2±0.2 mm are also applicable here. Of course, the second blind hole 12 can also be made slightly larger than the second through hole 21, or the second liquid injection member 8031 ​​can also be a hollow tube with an inner diameter D2 of 2±0.2 mm, to enhance the structural stability of the second waterproof ring 4 and improve the accuracy and flow rate stability of liquid injection.

[0047] In summary, the microfluidic detection sheet manufacturing method, microfluidic detection sheet and detection device of the present invention enable the microfluidic detection sheet to be combined by bonding processing of a first light guide sheet and a second light guide sheet. In the manufacturing process, a first blind hole and a first microfluidic channel are first formed on the first light guide sheet, and a first through hole is set at the corresponding position of the second light guide sheet. Before assembly, the first waterproof ring is placed between the first blind hole and the first through hole, and then the alignment and bonding of the light guide sheet are performed. This method and step not only ensures the precise alignment of each structure, but also because the first waterproof ring is buried between the first blind hole and the first through hole, it can effectively avoid the problem of traditional exposed sealing rings being easily displaced, falling off or causing liquid leakage due to stress deformation. What's more, in the manufacturing process, the first blind hole can be designed to be slightly larger than the first through hole, thereby further improving the stability of the placement of the first waterproof ring and the positioning tolerance during manufacturing. In addition, the bonding processing technology adopted can also promote the high tightness of the surfaces of the two light guide sheets, achieving a nearly seamless overall structure, and further improving the liquid-tight quality of the overall device. At the application level, the microfluidic detection piece can be used in combination with a fluid introduction element having a first liquid injection piece. The first liquid injection piece can be inserted into the first through hole so that the injected liquid can be smoothly introduced into the first blind hole and enter the first microchannel, completing a stable and precise liquid injection operation. In practice, in order to further improve the liquid injection efficiency and detection accuracy, the inner diameter of the first liquid injection piece can be specially set to 2±0.2mm. The setting of this inner diameter size can effectively balance the liquid flow rate and the inner wall contact area, so that the adsorption phenomenon between the liquid and the tube wall during the injection process is significantly reduced, thereby reducing the residual liquid. Furthermore, the present case can also be expanded to a structure with a second blind hole, a second through hole and a second microchannel, and a second waterproof ring and a second liquid injection piece are correspondingly provided to form multiple sets of independent liquid introduction channels. The second blind hole is provided on the first light guide plate, and the second through hole and the second microchannel are provided in the second light guide plate, so that multiple sets of microchannel structures can be layered, integrated and effectively aligned. This design allows the user to complete the assembly by simply aligning the corresponding holes and performing simple insertion, without the need for additional calibration or tool assistance, providing the convenience of quick plugging and unplugging. Combined with the layered processing and integrated bonding technology in the process, the overall manufacturing accuracy and sealing quality can be maintained even if the structure is complex. According to the detection requirements, the user can perform multiple groups of injection operations by quickly plugging and unplugging the corresponding injection parts, supporting the staggered combination of different reagents, samples or detection conditions, and improving the flexibility and modular application capabilities of the detection device. It is reiterated here that the present invention not only achieves high sealing, high stability and high application convenience in structural design, but also applies a layered structure, precise alignment and embedded waterproof ring solutions to ensure the accurate setting of the sealing elements, providing a comprehensive solution for the microfluidic detection system that combines structural innovation, process feasibility and operational convenience.

[0048] [Description of Reference Numerals]

[0049] 1. First light guide plate

[0050] 10 First blind hole

[0051] 11. First microfluidic channel

[0052] 12 Second blind hole

[0053] 2 Second light guide plate

[0054] 20 First through hole

[0055] 21 Second through hole

[0056] 22 Second microfluidic channel

[0057] 3. First waterproof ring

[0058] 4 Second waterproof ring

[0059] 8 Detection device

[0060] 80 Fluid introduction element

[0061] 801 plate body

[0062] 802 First injection structure

[0063] 8021 First injection part

[0064] 803 Second injection structure

[0065] 8031 Second injection part

[0066] 9 Microfluidic test piece

[0067] D1 Inner diameter of the first injection part

[0068] D2 Inner diameter of the second injection piece

[0069] Steps S11 to S14

[0070] Steps S21 to S24.

Claims

1. A method for manufacturing a microfluidic detection chip, characterized in that: The following steps are involved: A first processing step: forming at least one first blind hole and at least one first microchannel on a first light guide plate, wherein the first blind hole and the first microchannel are connected to each other; A second processing step: forming at least one first through hole on a second light guide plate, wherein the positions of the first through hole and the first blind hole correspond to each other; Placing step: placing a first waterproof ring between the first through hole and the first blind hole; as well as Bonding step: stacking the second light guide plate on the first light guide plate and bonding them to form a microchannel detection plate with the first waterproof ring buried therein.

2. The method for manufacturing a microfluidic detection sheet according to claim 1, wherein: The first processing step further includes forming at least one second blind hole on the first light guide plate; the second processing step further includes forming at least one second through hole and at least one second microchannel on the second light guide plate, wherein the second through hole and the second microchannel are interconnected, and the positions of the second through hole and the second blind hole correspond to each other; the placement step further includes placing a second waterproof ring between the second through hole and the second blind hole; The micro-channel detection sheet formed by the bonding step is further embedded with the second waterproof ring.

3. The method for manufacturing a microfluidic detection sheet according to claim 1, wherein: The first blind hole is larger than the first through hole, and in the placing step, the first waterproof ring is placed in the first blind hole.

4. A microfluidic detection chip, characterized in that: include: a first light guide plate having at least one first blind hole and at least one first microchannel, wherein the first blind hole and the first microchannel are interconnected; a second light guide plate, stacked on the first light guide plate and having at least one first through hole, wherein the first through hole and the first blind hole are located corresponding to each other, wherein the first light guide plate and the second light guide plate are integrated by bonding; and At least one first waterproof ring is buried between the first through hole and the first blind hole.

5. The microfluidic detection sheet according to claim 4, characterized in that: It also includes at least one second waterproof ring, and the first light guide plate also has at least one second blind hole, the second light guide plate also has at least one second through hole and at least one second microchannel, the second through hole and the second microchannel are connected to each other, and the positions of the second through hole and the second blind hole correspond to each other; the second waterproof ring is buried between the second through hole and the second blind hole.

6. The microfluidic detection sheet according to claim 4, characterized in that: The first blind hole is larger than the first through hole.

7. A detection device, characterized in that: include: A microfluidic detection sheet, comprising a first light guide sheet, a second light guide sheet, and at least one first waterproof ring, wherein the first light guide sheet has at least one first blind hole and at least one first microfluidic channel, and the first blind hole and the first microfluidic channel are interconnected; the second light guide sheet is stacked on the first light guide sheet and has at least one first through hole, the first through hole and the first blind hole corresponding to each other, wherein the first light guide sheet and the second light guide sheet are integrated by bonding; the first waterproof ring is buried between the first through hole and the first blind hole; and A fluid introduction element includes a plate body and at least a first injection structure. The first injection structure is arranged on the plate body and has a first liquid injection piece protruding from one side surface of the plate body, which is used to be inserted into the first through hole to inject liquid toward the first microchannel.

8. The detection device according to claim 7, characterized in that The microfluidic detection plate also includes at least one second waterproof ring, and the first light guide plate also has at least one second blind hole, the second light guide plate also has at least one second through hole and at least one second microchannel, the second through hole and the second microchannel are connected to each other, and the positions of the second through hole and the second blind hole correspond to each other; the second waterproof ring is buried between the second through hole and the second blind hole; the fluid introduction element also includes at least one second injection structure, the second injection structure is provided on the plate body and has a second liquid injection part protruding from one side surface of the plate body, which is used to be inserted into the second through hole to inject liquid toward the second microchannel.

9. The detection device according to claim 7, characterized in that: The first liquid injection part is a hollow tube structure with an inner diameter of 2±0.2 mm.

10. The detection device according to claim 7, characterized in that: The first blind hole is larger than the first through hole.

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