Experimental dyeing device for tissue slice

Abstract

The invention discloses an experimental staining device for tissue slices, which comprises an operating table, an operating turntable rotatably arranged on the operating table, and a plurality of vertically through placing holes positioned on the operating turntable, the placing holes are used for placing slice frames, and the operating table is provided with operating stations corresponding to the placing holes on the operating turntable. The operation station comprises a taking and placing station for loading and unloading the section frame, a dyeing station for dyeing the section frame, and a drying station for drying the section frame; a soaking cavity is formed in the dyeing station, tissue slices in the slice frame are dyed through lifting of the soaking cavity, and a receiving cavity is formed in the drying station and used for falling and collecting liquid, staying on the receiving cavity, of the slice frame; and the placing holes in the pick-and-place station are used for loading the slice frames to be dyed or unloading the dyed slice frames. Dyeing is more conveniently and efficiently completed, labor force is liberated, and the dyeing efficiency and quality are improved.

Description

Technical Field

[0001] The invention relates to the technical field of experimental equipment, in particular to an experimental staining device for tissue sections. Background Art

[0002] The biomedical field involves staining large quantities of tissue sections. Traditionally, slides carrying tissue sections are placed one by one into a staining jar and removed after staining. This entire manual process is tedious, time-consuming, and inefficient, placing a heavy workload on the operator. Therefore, if mechanized and automated staining could be implemented to free up manual labor, the overall efficiency of the experiment could be greatly improved. Summary of the Invention

[0003] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide an experimental staining device for tissue sections, which can transport and stain tissue sections in batches, thereby freeing up labor and improving staining efficiency and quality.

[0004] In order to solve the above technical problems, the present invention adopts the following technical solutions: an experimental staining device for tissue sections, comprising an operating table, an operating turntable rotatably arranged on the operating table, and a plurality of placement holes extending vertically on the operating turntable, wherein the placement holes are for placing a slice frame, and the operating table is provided with operating stations corresponding to the placement holes on the operating turntable, and the operating stations include a pick-up and place station for loading and unloading the slice frame, a staining station for staining the slice frame, and a drying station for drying the slice frame; an immersion chamber is provided at the staining station, and the lower part of the immersion chamber is controlled by a lifting mechanism to lift the immersion chamber, and the slice frame The bottom of the frame is a hollow structure for liquid to enter or discharge, and a plurality of placement slots are formed in a matrix in the slicing frame for placing slides carrying tissue slices. When the placement hole carrying the slicing frame rotates to the staining station, the immersion chamber containing the staining liquid is raised by the lifting mechanism to allow the slicing frame to be immersed in the immersion chamber, and then the liquid level of the staining liquid is higher than the slide to stain the tissue slice; a receiving chamber is provided at the drying station, and when the placement hole carrying the slicing frame rotates to the drying station and stops, the liquid falling on the slicing frame and the slide is collected by the receiving chamber; the placement hole at the pick-up and place station is used for loading the slicing frame to be stained or unloading the slicing frame after staining.

[0005] As an improvement, it also includes a loading and unloading turntable, which has a plurality of placement holes that pass through the upper and lower parts, and the placement holes on the operating turntable and the loading and unloading turntable pass through to the outside to form a material transfer trough; the loading and unloading turntable and the operating turntable are arranged adjacent to each other, and the loading and unloading turntable is located on the side corresponding to the pick-up and placement station, and a material transfer mechanism is arranged below the loading and unloading turntable and the operating turntable, and the material transfer mechanism includes a track, a material transfer seat, a lifting cylinder and a lifting seat, and the track is arranged below the loading and unloading turntable and the operating turntable, and the material transfer seat is slidably arranged on the track The lifting seat is driven by the lifting cylinder to move along the track, and the driving mechanism is used to move the material moving seat. The lifting cylinder is arranged on the material moving seat, and the lifting seat is arranged on the upper end of the cylinder shaft of the lifting cylinder. When in use, the material moving seat corresponds to the bottom of the placement hole of the loading and unloading turntable or the operating turntable, and the lifting seat is driven by the lifting cylinder to rise to lift the slicing frame out of the placement hole. Then the material moving seat slides along the track and the cylinder shaft moves along the material moving trough to transfer the slicing frame between the loading and unloading turntable and the operating turntable. The slicing frame is placed at the placement hole of the operating turntable or the loading and unloading turntable after the transfer is completed.

[0006] As an improvement, three placement holes are evenly arranged along the circumference on the operating turntable and the loading and unloading turntable.

[0007] As an improvement, the lower inner wall of the soaking chamber is connected to an outlet pipe for discharging waste liquid, and the upper inner wall is connected to an inlet pipe for replenishing new liquid.

[0008] As an improvement, the lower inner wall of the receiving cavity is externally connected to a waste liquid pipeline for waste liquid discharge and a circulation pipeline for liquid recycling.

[0009] As an improvement, a fan is provided directly above the drying station. When the placement hole carrying the slicing frame rotates to the drying station and stops, the fan blows air to dry the slicing frame below.

[0010] As an improvement, the placement groove forms a card wall on a pair of sides, and a placement eaves is set below the card wall, and the placement eaves are set one high and one low. When the slide is placed in the placement groove, it is placed on the placement eaves in an inclined state and the opposite side is limited by the card wall.

[0011] As an improvement, the slicing frame includes an upper wall and a lower wall, the upper periphery of the upper wall forms an upper eaves, the upper wall is placed in the placement hole and the upper eaves is supported on the upper surface of the operating turntable; the lower wall extends downward from the placement hole and is distributed with a hollow structure, and the placement groove is located on the lower wall.

[0012] The beneficial effects achieved by the present invention are:

[0013] 1. Through simple structural design, it replaces manual labor to dye and dry batches of tissue sections, and then performs loading and unloading operations. Compared with the traditional method of dyeing one by one, it is more convenient and efficient, liberates labor, and improves dyeing efficiency and quality.

[0014] 2. Reduce the workload of operators and transform the original boring operations into equipment control. Operators can take care of other laboratory work and improve their efficiency.

[0015] 3. The overall equipment uses a circular operation method, which makes the equipment take up less space and is convenient for laboratory accommodation and maintenance management. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic top view of the structure of the first embodiment of the device of the present invention.

[0017] Figure 2 It is a schematic diagram of the longitudinal cross-sectional structure of the first embodiment of the device of the present invention.

[0018] Figure 3 It is a schematic top view of the second embodiment of the device of the present invention.

[0019] Figure 4 It is a schematic diagram of the longitudinal cross-sectional structure of the second embodiment of the device of the present invention.

[0020] Figure 5 It is a partial longitudinal cross-sectional structural diagram of the slicing frame of the device of the present invention.

[0021] In the figure: 0, loading and unloading turntable; 1, operating table; 2, operating turntable; 3, placement hole; 31, material transfer trough; 4, slicing frame; 401, upper wall; 402, lower wall; 41, placement trough; 42, clamping wall; 43, placement eaves; 44, upper eaves; 5, operating station; 51, pick-up and placement station; 52, dyeing station; 53, drying station; 6, soaking chamber; 61, lifting mechanism; 62, liquid inlet pipeline; 63, liquid outlet pipeline; 7, receiving chamber; 71, waste liquid pipeline; 72, circulation pipeline; 73, fan; 8, material transfer mechanism; 81, track; 82, material transfer seat; 83, lifting cylinder; 84, lifting seat. DETAILED DESCRIPTION

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] like Figure 1 、 2, 3, 4, and 5 show an embodiment of the experimental staining device for tissue slices provided by the present invention, which comprises an operating table 1, an operating turntable 2 rotatably arranged on the operating table 1, and a plurality of placement holes 3 that pass through the operating turntable 2. The placement holes 3 are for placing the slice frame 4. The operating table 1 is provided with operating stations 5 corresponding to the placement holes 3 on the operating turntable 2. The operating stations 5 comprise a taking and placing station 51 for loading and unloading the slice frame 4, a staining station 52 for staining the slice frame 4, and a drying station 53 for drying the slice frame 4. An immersion chamber 6 is provided at the staining station 52. The lower part of the immersion chamber 6 is controlled by a lifting mechanism 61. The slice frame The bottom of 4 is a hollow structure for liquid to enter or discharge. A plurality of placement slots 41 are formed in a matrix in the slicing frame 4 for placing the slide glass carrying the tissue slices. When the placement hole 3 carrying the slicing frame 4 rotates to the staining station 52, the immersion chamber 6 containing the staining liquid is raised by the lifting mechanism 61 to allow the slicing frame 4 to be immersed in the immersion chamber 6, and then the staining liquid level is higher than the slide glass to stain the tissue slice; a receiving chamber 7 is provided at the drying station 53. When the placement hole 3 carrying the slicing frame 4 rotates to the drying station 53 and stops, the liquid falling on the slicing frame 4 and the slide glass is collected by the receiving chamber 7; the placement hole 3 at the pick-up and place station 51 is used for loading the slicing frame 4 to be stained or unloading the slicing frame 4 after staining.

[0024] When the present invention is in use, the operating table 1 is supported on the ground by a frame or box structure below, so that the operating table 1 and the operating turntable 2 are at a suitable height position for easy operation by personnel, and the lower frame or box structure is convenient for accommodating the mechanical components and electrical components at the bottom of the operating table 1. The operating turntable 2 can be controlled to rotate by a servo motor, and the operating station 5 is specifically set as a pick-up and placement station 51, a staining station 52 and a drying station 53. The three are evenly arranged, and then when the servo motor controls the operating turntable 2 to rotate, it rotates 120 degrees each time, and then the placement hole 3 and the slice frame 4 placed therein can be input to the next position station. The slide containing the tissue section to be stained is pre-placed by personnel into the placement slot 41 of the slice frame 4, and an n*n array of placement slots 41 is set as needed (for example Figure 1The 3*3 placement slots 41 shown are used to clamp batches of slides in the placement slots 41 for positioning. The slot wall material of the placement slots 41 can be made of a plastic material with deformation properties, so that the slides can be clamped by a certain degree of deformation interference. The slicing frame 4 is placed in the placement hole 3 from the pick-up and placement station 51. When the operating turntable 2 is running, the slicing frame 4 is transported to the staining station 52 for storage. The immersion chamber 6 at the staining station 52 is driven up by the lifting mechanism 61 to allow the slicing frame 4 to be immersed in the immersion chamber 6. The immersion chamber 6 is pre-filled with dyeing liquid. The rising height makes the liquid level of the dyeing liquid higher than the slide, thereby staining the tissue section; the lifting mechanism 61 can be specifically implemented by a cylinder, and when in use, the cylinder can be set to a slowly rising state, so that the immersion chamber 6 and the dyeing liquid therein slowly overflow the slide, which is conducive to the dyeing liquid filling the interior of the slicing frame 4 through the hollow structure at the bottom of the slicing frame 4 and fully contacting the slide to stain the tissue section thereon, without causing the liquid to impact the tissue section. The hollow structure at the bottom of the slicing frame 4 can be specifically set to a uniformly distributed hole structure to ensure the smoothness of the liquid passing through. After soaking for a predetermined time, the soaking chamber 6 is driven by the lifting mechanism 61 to descend and reset, and then the turntable 2 is operated to transport the slicing frame 4 to the drying station 53 for a while. The slicing frame 4 is allowed to stay at the drying station 53 for a certain period of time, so that the residual liquid on the slide and the slicing frame 4 is dripped and collected. The liquid is caught and collected by the receiving chamber 7. The stay process is determined by the time of dyeing the next batch of tissue slices at the previous station. After the drying is completed (after the previous station completes the dyeing), the turntable 2 is operated to transport the slicing frame 4 back to the pick-up and place station 51, and the batch of tissue slices that have been dyed and dried can be output together with the slicing frame 4 for unloading, and then a new slicing frame 4 to be dyed is replaced to carry out the next cycle process. During the use of the device, the loading and unloading station 51, the dyeing station 52 and the drying station 53 can each complete their own functions without affecting each other. That is, when one batch of tissue slices is dyeing, the previous batch of tissue slices is drying, and the previous and next batches of tissue slices are loading and unloading. This greatly improves the operating efficiency, greatly improving the dyeing efficiency compared to the original one-by-one operation method, and simplifies the tedious operation of personnel. By setting the dyeing time in a more standardized way, the dyeing quality of each batch can be guaranteed.

[0025] As an improved specific embodiment, it also includes a loading and unloading turntable 0, which has a plurality of placement holes 3 that pass through the upper and lower parts, and the placement holes 3 on the operating turntable 2 and the loading and unloading turntable 0 pass through to the outside to form a material transfer groove 31; the loading and unloading turntable 0 and the operating turntable 2 are arranged adjacent to each other, and the loading and unloading turntable 0 is located on the side corresponding to the pick-up and placement station 51, and a material transfer mechanism 8 is provided below between the loading and unloading turntable 0 and the operating turntable 2. The material transfer mechanism 8 includes a track 81, a material transfer seat 82, a lifting cylinder 83 and a lifting seat 84. The track 81 is arranged below between the loading and unloading turntable 0 and the operating turntable 2, and the material transfer seat 82 is slidably provided on the track The material moving seat 82 is arranged on the track 81 and moves along the track 81 through the driving mechanism. The lifting cylinder 83 is arranged on the material moving seat 82, and the lifting seat 84 is arranged on the upper end of the cylinder shaft of the lifting cylinder 83; when in use, the material moving seat 82 corresponds to the bottom of the placement hole 3 of the loading and unloading turntable 0 or the operating turntable 2, and the lifting cylinder 83 drives the lifting seat 84 to rise and lift the slicing frame 4 out of the placement hole 3, then the material moving seat 82 slides along the track 81 and the cylinder shaft moves along the material moving groove 31, transferring the slicing frame 4 between the loading and unloading turntable 0 and the operating turntable 2, and the slicing frame 4 is placed on the placement hole 3 of the operating turntable 2 or the loading and unloading turntable 0 after the transfer is completed.

[0026] like Figure 3 、 4As shown, to further enhance the convenience and automation of loading and unloading, and to free up labor, a loading and unloading turntable 0 and a material transfer mechanism 8 are provided. By depositing multiple sets of slicing frames 4 loaded with tissue sections to be stained on the loading and unloading turntable 0, the system can automatically complete the staining of the stored slicing frames 4 over a period of time. The material transfer mechanism 8 facilitates the transfer of the slicing frames 4 between the loading and unloading turntable 0 and the operating turntable 2. The placement holes 3 and the borders of the slicing frames 4 are designed to be matching rectangles, thus ensuring stable storage. During the transfer process, a stained and dried slice frame 4 moves to the loading and unloading station 51. The transfer seat 82 waits for the slice frame 4 at the loading and unloading station 51. The lifting cylinder 83 drives the lifting seat 84 upward to lift the slice frame 4 out of the placement hole 3. The lifting cylinder 83 then drives the lifting seat 84 upward to lift the slice frame 4 out of the placement hole 3. The lifting cylinder 83 drives the lifting seat 84 downward to place the slice frame 4 in the placement hole 3, completing the unloading of the slice frame 4. After the unloading and unloading turntable 0 rotates, the next slice frame 4 to be loaded moves to the loading position. The transfer mechanism 8 repeats the above action, transferring the slice frame 4 from the unloading and unloading turntable 0 to the operating turntable 2. The number of placement holes 3 on the unloading and unloading turntable 0 is set as needed to accommodate a sufficient number of slice frames 4. After the personnel pre-place the slice frames 4, the automatic operation can be performed for a certain period of time to complete the staining of all tissue sections, thus freeing up labor and allowing personnel to perform other tasks during the process, thus improving efficiency. By providing a material transfer groove 31 outside the placement hole 3 , the cylinder shaft of the lifting cylinder 83 can be moved between two opposite placement holes 3 .

[0027] As an improved specific implementation method, three placement holes 3 are evenly arranged along the circumference on the operating turntable 2 and the loading and unloading turntable 0.

[0028] like Figure 3 As shown, the operating turntable 2 is evenly provided with three placement holes 3 corresponding to the pick-up and placement station 51, the dyeing station 52 and the drying station 53, so as to realize the synchronous process of each station. The loading and unloading turntable 0 and the operating turntable 2 are set to the same structure, and then unified mold opening and manufacturing can be achieved to achieve cost control and simplification of equipment use and maintenance.

[0029] As an improved specific implementation manner, the lower inner wall of the soaking chamber 6 is externally connected to a liquid outlet pipe 63 for discharging waste liquid, and the upper inner wall is externally connected to a liquid inlet pipe 62 for replenishing new liquid.

[0030] like Figure 2As shown, during specific implementation, the lifting mechanism 61 specifically adopts a cylinder part, and a tray is set at the upper end of the cylinder shaft of the cylinder part. The tray is used to place the immersion chamber 6. The immersion chamber 6 and the tray can be detachably matched through a locking structure, thereby achieving the effect of stabilizing the immersion chamber 6. During the use of the immersion chamber 6, if the dyeing liquid needs to be replaced after long-term use, the waste liquid can be discharged through the liquid outlet pipe 63, and then new liquid can be added through the liquid inlet pipe 62, and then the dyeing liquid can be replaced without removing the immersion chamber 6. Soft materials can be used for the liquid inlet pipe 62 and the liquid outlet pipe 63. Under the premise of completing the component docking and infusion, they will not be affected by the lifting and lowering displacement of the immersion chamber 6. When the machine is shut down and not in use, the immersion chamber 6 can be disassembled and cleaned.

[0031] As an improved specific implementation method, the lower inner wall of the receiving chamber 7 is externally connected to a waste liquid pipeline 71 for waste liquid discharge and a circulation pipeline 72 for liquid recycling.

[0032] like Figure 2 As shown, the dye liquid collected in receiving chamber 7 is determined by personnel to determine its reusability. If it is recyclable, it is transported back to the collection chamber via circulation line 72 and then re-transferred to the liquid inlet line 62. If it has been used for a long time and is close to being waste liquid, it is transported to the waste liquid tank via waste liquid line 71. Receiving chamber 7 is detachably mounted on the operating table 1 and can be disassembled and cleaned when the machine is not in use.

[0033] As an improved specific implementation method, a fan 73 is also provided directly above the drying station 53. When the placement hole 3 carrying the slicing frame 4 rotates to stop at the drying station 53, the fan 73 blows air to dry the slicing frame 4 below.

[0034] like Figure 2 As shown, the liquid will hang on the wall and remain in the corners when there is no external force. By further arranging a fan 73 on the top, the fan 73 provides a soft blowing of appropriate size to accelerate and promote the blowing and falling of the liquid remaining on the slice frame 4 and the slide to complete the drying; in the specific implementation, cold air is used to avoid damage to the tissue slices.

[0035] As an improved specific embodiment, the placement groove 41 forms a card wall 42 on a pair of sides, and a placement eaves 43 is set below the card wall 42, and the placement eaves 43 are set one high and one low. When the slide is placed in the placement groove 41, it is placed on the placement eaves 42 in an inclined state and the opposite side is limited by the card wall 42.

[0036] like Figure 5As shown, in a specific implementation, the left and right clamping walls 42 as shown in the figure are adapted to the size of the glass slide, so that the glass slide is interference-locked between the left and right clamping walls 42, and the front and rear inner walls are set larger than the size of the glass slide, which is convenient for people to pick up the glass slide from both sides with their fingers and to push the glass slide out from the bottom; and the placement eaves 43 set one high and one low make the glass slide in an inclined state, so that the dyeing liquid can flow along the inclined state, thereby improving the flow and dyeing effect of the dyeing liquid, and facilitating the downward flow and discharge of the liquid when it is dried.

[0037] As an improved specific embodiment, the slicing frame 4 includes an upper wall 401 and a lower wall 402, the upper periphery of the upper wall 401 forms an upper eaves 44, the upper wall 401 is placed in the placement hole 3 and the upper eaves 44 is supported on the upper surface of the operating turntable 2; the lower wall 402 extends downward from the placement hole 3 and is distributed with a hollow structure, and the placement groove 41 is located on the lower wall 402.

[0038] like Figure 2 As shown, a circle of upper eaves 44 is supported on the upper surface of the operating turntable 2, providing stable placement of the slice frame 4; the upper wall 401 cooperates with the inner wall of the placement hole 3 to provide structural stability and limitation; the lower wall 402 extends downward from the placement hole 3 and is distributed with hollow holes. When the immersion chamber 6 rises, it can fully immerse the height of the lower wall 402, and then fully immerse the placement groove 41 located at the position of the lower wall 402 and the slide carrying the tissue slice, so as to achieve a sufficient staining effect.

[0039] The above are merely preferred embodiments of the present invention. The scope of protection of the present invention is not limited to the above embodiments. All technical solutions based on the principles of the present invention are within the scope of protection of the present invention. It should be noted that improvements and modifications that do not depart from the principles of the present invention are within the scope of protection of the present invention.

Claims

1. An experimental staining device for tissue sections, characterized in that: The invention comprises an operating table (1), an operating turntable (2) rotatably arranged on the operating table (1), and a plurality of placement holes (3) on the operating turntable (2) that are connected vertically, wherein the placement holes (3) are for placing a slicing frame (4). The operating table (1) is provided with operating stations (5) corresponding to the placement holes (3) on the operating turntable (2). The operating stations (5) comprise a loading and unloading station (51) for loading and unloading the slicing frame (4), a dyeing station (52) for dyeing the slicing frame (4), and a drying station (53) for drying the slicing frame (4); a soaking chamber (6) is provided at the dyeing station (52), and a lifting mechanism (61) is provided at the lower part of the soaking chamber (6) to control the lifting of the soaking chamber (6). The bottom of the slicing frame (4) is a hollow structure. The liquid is then allowed to enter or be discharged. A plurality of placement slots (41) are formed in a matrix in the slicing frame (4) for placing the glass slide carrying the tissue slice. When the placement hole (3) carrying the slicing frame (4) rotates to the staining station (52), the soaking chamber (6) containing the staining liquid is raised by the lifting mechanism (61) to allow the slicing frame (4) to be soaked in the soaking chamber (6), thereby making the staining liquid level higher than the glass slide to stain the tissue slice. A receiving chamber (7) is provided at the drying station (53). When the placement hole (3) carrying the slicing frame (4) rotates to the drying station (53) and stops, the liquid falling on the slicing frame (4) and the glass slide is collected by the receiving chamber (7). The placement hole (3) at the pick-up and place station (51) is used for loading the slicing frame (4) to be stained or unloading the slicing frame (4) after staining.

2. The tissue section staining device according to claim 1, characterized in that: The loading and unloading turntable (0) is provided with a plurality of placement holes (3) extending vertically therethrough, and the placement holes (3) on the operating turntable (2) and the loading and unloading turntable (0) extend outwardly to form a material transfer groove (31); the loading and unloading turntable (0) and the operating turntable (2) are arranged adjacent to each other, and the loading and unloading turntable (0) is located on the side corresponding to the pick-up and placement station (51), and a material transfer mechanism (8) is provided below between the loading and unloading turntable (0) and the operating turntable (2), and the material transfer mechanism (8) includes a track (81), a material transfer seat (82), a lifting cylinder (83) and a lifting seat (84), the track (81) is arranged below between the loading and unloading turntable (0) and the operating turntable (2), and the material transfer seat (82) is slidably provided on the track (81). ) and moves along the track (81) through a driving mechanism, the lifting cylinder (83) is arranged on the material transfer seat (82), and the upper end of the cylinder shaft of the lifting cylinder (83) is provided with a lifting seat (84); when in use, the material transfer seat (82) corresponds to the lower part of the placement hole (3) of the loading and unloading turntable (0) or the operating turntable (2), and the lifting seat (84) is driven by the lifting cylinder (83) to rise and lift the slicing frame (4) out of the placement hole (3), and then the material transfer seat (82) slides along the track (81) and the cylinder shaft moves along the material transfer groove (31), so as to transfer the slicing frame (4) between the loading and unloading turntable (0) and the operating turntable (2), and the slicing frame (4) is placed at the placement hole (3) of the operating turntable (2) or the loading and unloading turntable (0) after the transfer is completed.

3. The tissue section staining device according to claim 2, characterized in that: Three placement holes (3) are evenly arranged on the operating turntable (2) and the loading and unloading turntable (0) along the circumference.

4. The tissue section staining device according to claim 1, characterized in that: The lower inner wall of the soaking chamber (6) is externally connected to a liquid outlet pipeline (63) for discharging waste liquid, and the upper inner wall is externally connected to a liquid inlet pipeline (62) for replenishing new liquid.

5. The tissue section staining device according to claim 1, characterized in that: The lower inner wall of the receiving cavity (7) is externally connected to a waste liquid pipeline (71) for waste liquid discharge and a circulation pipeline (72) for liquid circulation.

6. A tissue section staining device according to any one of claims 1 to 5, characterized in that: A fan (73) is also provided just above the drying station (53). When the placement hole (3) carrying the slicing frame (4) rotates to the drying station (53) and stops, the fan (73) blows air to dry the slicing frame (4) below.

7. A tissue section staining device according to any one of claims 1 to 5, characterized in that: The placement groove (41) forms a card wall (42) on a pair of sides, and a placement eaves (43) is provided below the card wall (42). The placement eaves (43) are arranged one high and one low. When the slide is placed in the placement groove (41), it is placed on the placement eaves (42) in an inclined state and the opposite side is restrained by the card wall (42).

8. A tissue section staining device according to any one of claims 1 to 5, characterized in that: The slicing frame (4) comprises an upper wall (401) and a lower wall (402); the upper periphery of the upper wall (401) forms an upper eaves (44); the upper wall (401) is placed in the placement hole (3) and the upper eaves (44) is supported on the upper surface of the operating turntable (2); the lower wall (402) extends downward from the placement hole (3) and is distributed with a hollow structure; the placement groove (41) is located on the lower wall (402).

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