Technology for refrigerating and delaying germination of sandy nursery stocks

Abstract

The invention discloses a desert nursery stock refrigeration delayed germination technology, and particularly relates to the technical field of desert nursery stock storage, and the desert nursery stock refrigeration delayed germination technology comprises the following steps: placing a nursery stock root cluster with native sandy soil in a container containing a phase change cold storage matrix, sequentially carrying out biological rhythm regulation and control of dark low-temperature induction and specific blue light time sequence activation, and implementing zoned gradient recovery; a special refrigeration device is matched, a bionic library body keeps the microenvironment stable, a rotary cultivation frame balances cold distribution, and a multi-source sensing monitoring system evaluates the physiological state of the nursery stock in real time. Through light and temperature cooperative control and root system micro-ecology preservation technologies, accurate delay of the germination period of the sandy nursery stocks and effective improvement of the planting survival rate are achieved, and the technical problem that the afforestation window period of the sandy area is not matched with nursery stock supply is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of storage of desert seedlings, and more particularly to a cold storage and delayed germination technology of desert seedlings. Background Art

[0002] In ecological governance projects in the arid regions of northwest my country, the large-scale planting of sand-dwelling seedlings (such as Haloxylon ammodendron, Salix psammophila, and Elaeagnus chinensis) faces a core contradiction: a short seasonal construction window, a concentrated natural germination period, and a mismatch between the project schedule. Especially in major national ecological projects such as the "Three North Shelterbelt Project," more than 25% of high-quality seedlings are abandoned each year because the spring planting period is less than 30 days. Although traditional open-air transplanting can temporarily delay germination, the survival rate of seedlings is generally less than 65% due to the drastic temperature and humidity fluctuations in the sandy areas (the temperature difference between day and night can reach more than 30°C) and strong wind and sand erosion. With the 2021 "Technical Guidelines for Ecological Restoration in Sandy Areas" mandating a afforestation survival rate of ≥85%, the industry urgently needs a technical solution that can accurately control the physiological rhythms of seedlings and achieve flexible seedling supply across seasons. In this context, the cold storage delayed germination technology based on physiological regulation of a controlled environment has become a key breakthrough in solving the problem of temporal and spatial mismatch in afforestation in sandy areas: The current mainstream seedling cold storage delayed germination technology has three major systemic defects: First, general cold storage uses a uniform low-temperature environment (usually -2~4℃), ignoring the unique low-temperature response mechanism of desert plants. For example, Haloxylon ammodendron seedlings will trigger cell membrane lipid phase changes in an environment continuously below 0℃, resulting in a surge of more than 40% in electrolyte leakage rate after recovery; Second, the bare-root cold storage method used by existing technologies destroys the rhizosphere microbial community, causing the mycorrhizal fungal colonization rate to drop by 72%, seriously weakening the seedlings' stress resistance; Third, the traditional recovery process uses a direct warming mode, causing the recovery of mitochondrial function to lag behind chloroplast activity, triggering an explosive accumulation of reactive oxygen species. More importantly, the existing technology completely ignores the regulation of the light environment, resulting in the maximum quantum yield (Fv / Fm) of the photosystem II of the seedlings after cold storage to drop below 0.65 (healthy value>0.8). Statistics from the 2023 "Arid Area Forestry Science and Technology" show that the conventional technology delays germination by 7±3 days, and the mortality rate of seedlings within 30 days after leaving the warehouse is as high as 35%, which cannot meet the needs of precise afforestation; Therefore, in response to the above problems, a technology for cold storage and delayed germination of desert seedlings is proposed. Summary of the Invention

[0003] In order to overcome the above-mentioned defects of the prior art, an embodiment of the present invention provides a cold storage delayed germination technology for desert seedlings to solve the problems raised in the above-mentioned background technology.

[0004] To achieve the above object, the present invention provides the following technical solution: a method for cold storage and delayed germination of psammophyte seedlings, comprising the following consecutive steps: excavating psammophyte seedlings in a deep dormant period together with the original sandy soil at their roots to form a ball-shaped root ball, wherein the root ball diameter is controlled within the range of 18±2 times the seedling ground diameter, and the original sandy soil retention is ≥95%; placing the ball-shaped root ball in a humidity-controlled container filled with a cold storage matrix, wherein the cold storage matrix is ​​composed of vermiculite, diatomaceous earth, and a nano-organic phase change material with a phase change temperature of -0.8°C at 6 :3:1 mass ratio, with the moisture content maintained at 10±2%; the container containing the root ball was placed in a dark, sealed environment at -1℃ to 0.5℃ for the first stage of induced cold storage, which lasted for 40±5 days, during which the carbon dioxide concentration was maintained at 1000±200ppm and the relative humidity was 88±2%; then the container was transferred to a weak light environment at -0.3℃ to 1℃ for the second stage of physiological activation, with a wavelength of 460±10nm and an intensity of 12±3μmol·m -2 ·s -1 The plants were irradiated with blue light, and the irradiation period was synchronized with the sunrise time in the original habitat of the seedlings; finally, gradient recovery treatment was carried out in a transitional environment of 3.5℃±1.5℃, with a daily heating rate not exceeding 0.3℃ / hour, and the relative humidity was linearly reduced from 85% to 68%, which lasted for 6±1 days.

[0005] Preferably, the process of forming the spherical root ball includes retaining the main root system length of not less than 40 cm during excavation, controlling the lateral root damage rate below 5%, wrapping the root ball with degradable non-woven fabric and using plant adhesive to fix the sand structure, the adhesive component is a composite gel of sodium alginate and montmorillonite in a ratio of 7:3, and the oxygen permeability after curing reaches more than 90%.

[0006] Preferably, the preparation of the cold storage matrix includes encapsulating nano-organic phase change material in porous silica microcapsules to form slow-release particles with a particle size of 0.5-1 mm, and the diatomaceous earth is heat-treated at 300°C to activate the microporous structure. After mixing, the cold storage capacity of the matrix at -1°C environment is 3.2 times that of conventional sand, and the temperature fluctuation range is ≤±0.2°C.

[0007] Preferably, the first stage induces a cold storage environment and simultaneously configures a negative oxygen ion generator to maintain an air negative ion concentration of 800-1200 / cm 3 , and periodically input sound wave stimulation with a frequency of 28kHz, 3 times a day, 20 minutes each time, and the sound intensity is controlled below 55dB; during the second stage of physiological activation, atomized nutrient solution containing 5-aminolevulinic acid is injected simultaneously, with a concentration of 0.1mmol / L, once every 48 hours.

[0008] Preferably, the gradient resuscitation treatment adopts a zoned temperature control strategy, dividing the transition environment into three temperature zones: the first temperature zone is 3.5℃±0.5℃, humidity is 80±5%, and stays for 24 hours; the second temperature zone is 5℃±0.5℃, humidity is 75±3%, and stays for 48 hours; the third temperature zone is 7℃±1℃, humidity is 68±2%, and stays for 72 hours; the air flow rate in each temperature zone is maintained at a directional circulation of 0.1-0.3m / s.

[0009] Preferably, stress resistance strengthening treatment is carried out 24 hours before shipment, including spraying a compound solution containing 0.05% betaine and 0.1% silicon potassium minerals on the leaves, perfusing the roots with pH 6.5 phosphate buffer, and applying 14 hours of red light irradiation with a wavelength of 660nm to activate the light protection mechanism.

[0010] A special refrigeration system includes: an insulated storage body with a bionic sand surface texture, the inner wall of which is provided with a micro-topography groove structure with a depth of 0.8±0.3mm; a programmable temperature control system that can achieve precise regulation of ±0.1℃; a rotating cultivation rack with an adjustable tilt angle of 5-12°, which completes 360° uniform rotation every 120 minutes; a root ball fixing device equipped with a dual-mode monitoring unit, in which a near-infrared spectral probe detects changes in root moisture content in real time, and an electrical impedance sensor dynamically tracks cell membrane integrity; a spectrally tunable light source system provides continuously adjustable light with a wavelength of 400-700nm, and a built-in sunrise simulation timing controller.

[0011] Preferably, the rotating cultivation rack is equipped with a gravity balance compensation mechanism, which automatically adjusts the position of the counterweight block to maintain dynamic stability when the rotation speed changes; the surface of the cultivation rack is covered with an elastic base pad with shape memory function, and an array of air permeable holes with a diameter of 2-5mm is distributed on the surface. The hole density increases with increasing temperature, and the porosity reaches 35% at 5°C.

[0012] Preferably, the dual-mode monitoring unit integrates an artificial intelligence analysis module, which associates the near-infrared absorption peak positions (1450nm and 1940nm) with the electrical impedance phase angle data through a machine learning algorithm to establish a seedling vitality prediction model, and automatically triggers a supplementary processing program when the predicted germination potential is lower than the threshold.

[0013] Preferably, the outer layer of the insulated warehouse body is made of a composite phase change insulation board, embedded with paraffin / graphene composite phase change material, with a phase change range of -5°C to 10°C and a thermal inertia index D value ≥8.0; the warehouse door is provided with a double-channel airtight locking device, and a magnetic fluid sealing strip is embedded in the door gap, with a cooling leakage rate ≤0.05W / m·K.

[0014] Technical effects and advantages of the present invention: Compared with the existing technology, the present invention constructs a native sandy soil root ball structure combined with a degradable adhesive solid to completely preserve the root microecological environment, and uses the temperature buffering characteristics of the phase change cold storage matrix to achieve ±0.2°C precise temperature control; innovatively adopts a two-stage light and temperature coordinated regulation mechanism, maintains darkness and low temperature to inhibit metabolism during the deep dormancy period, and introduces specific wavelength blue light timed irradiation during the physiological activation stage to synchronize the biological clock rhythm; through the partitioned gradient recovery design coupled with stress strengthening treatment, the synchronous activation of the mitochondrial respiratory chain enzyme system and the photosynthetic organs is achieved; with the help of near-infrared-electrical impedance multimodal sensing and machine learning algorithms, a dynamic evaluation model for seedling vitality is established, and finally the core advantages of sand-dwelling seedlings are achieved, that is, the physiological activity is stably maintained during an ultra-long cold storage period, the timing of leaving the warehouse is precisely controlled, and the survival rate after planting is significantly improved, which fundamentally solves the problem of temporal and spatial mismatch between seedling supply and afforestation window period in sandy area ecological projects. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is the workflow diagram of the present invention.

[0016] Figure 2 It is the overall framework structure diagram of the present invention.

[0017] Figure 3 This is a diagram of the environmental control system of the present invention. DETAILED DESCRIPTION

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] Example 1 As attached Figure 1-3As shown, (1) a method for cold storage and delayed germination of psammophyte seedlings, comprising the following consecutive steps: digging the psammophyte seedlings in deep dormancy together with the original sandy soil at the roots to form a ball-shaped root ball, wherein the root ball diameter is controlled within the range of 18±2 times the seedling ground diameter, and the original sandy soil retention is ≥95%; placing the ball-shaped root ball in a humidity-controlled container filled with a cold storage matrix, wherein the cold storage matrix is ​​composed of vermiculite, diatomaceous earth and nano-organic phase change material with a phase change temperature of -0.8°C in a mass ratio of 6:3:1 The water content was kept constant at 10±2%. The container containing the root ball was placed in a dark, airtight environment at -1°C to 0.5°C for the first stage of induction cold storage for 40±5 days, during which the carbon dioxide concentration was maintained at 1000±200ppm and the relative humidity was 88±2%. The container was then transferred to a weak light environment at -0.3°C to 1°C for the second stage of physiological activation, applying light at a wavelength of 460±10nm and an intensity of 12±3μmol·m -2 ·s -1 The blue light irradiation was synchronized with the sunrise time in the original place of the seedlings; finally, the gradient recovery treatment was carried out in a transitional environment of 3.5℃±1.5℃, with a daily heating rate of no more than 0.3℃ / hour, and the relative humidity was linearly reduced from 85% to 68% for 6±1 days. Among them, the Haloxylon ammodendron seedlings with a ground diameter of 1.5 cm were dug in the deep dormancy period (late December), and the root balls with a diameter of 27±3 cm were retained (the original sandy soil accounted for ≥95%) and placed in a humidity-controlled cold storage matrix filled with cold storage matrix. The container was a matrix composed of vermiculite (particle size 2-4 mm), diatomaceous earth (activated at 300°C), and nano-octacosanoic acid phase change material (microencapsulated particle size 0.8 mm) in a ratio of 6:3:1, with a moisture content of 10%. The container was placed in a dark environment at -0.5°C ± 0.2°C for 45 days (CO2 concentration 1000 ppm, humidity 90%). It was then moved to a low-light environment at 0.5°C and exposed to 460 nm blue light (intensity 12 μmol·m -2 ·s -1 ); Finally, it was revived in the 3.5℃ transition zone for 6 days: humidity 85%→80% on the 1st and 2nd days, 5℃ / humidity 75% on the 3rd and 4th days, 7℃ / humidity 68% on the 5th and 6th days, with a heating rate of ≤0.3℃ / h throughout the whole process.

[0020] (2) The process of forming the spherical root ball includes retaining the main root system length of not less than 40 cm during excavation, controlling the lateral root damage rate to less than 5%, wrapping the root ball with a degradable non-woven fabric and using a plant adhesive to fix the sand structure, the adhesive component is a composite gel of sodium alginate and montmorillonite in a ratio of 7:3, and the oxygen permeability after solidification is more than 90%, wherein, when the root ball is excavated, an arc shovel is used to protect the main root (length ≥ 40 cm), and the lateral root pruning rate is controlled at 3%; spraying the sodium alginate-montmorillonite composite gel (7:3 ratio) on the surface of the root ball to form a 0.5 mm breathable film, and wrapping it with a degradable hemp fiber non-woven fabric (gram weight 80g / m 2 The adhesive forms a porous network structure (pore size 50-200μm) after calcium ion cross-linking, and the oxygen transmission rate reaches 92mL / (m 2 h) Ensure that the survival rate of rhizosphere microorganisms (such as arbuscular mycorrhizal fungi) is greater than 90%.

[0021] (3) The preparation of the cold storage matrix includes encapsulating nano organic phase change material in porous silica microcapsules to form slow-release particles with a particle size of 0.5-1 mm. The diatomaceous earth is heat-treated at 300 ° C to activate the microporous structure. After mixing, the cold storage capacity of the matrix at -1 ° C environment is 3.2 times that of conventional sand, and the temperature fluctuation range is ≤ ± 0.2 ° C. Among them, the cold storage matrix is ​​prepared by encapsulating nano octadecanoic acid (phase change point -0.8 ° C) in porous silica microcapsules (wall thickness 0.1 mm) through fluidized bed coating technology; the diatomaceous earth is calcined at 300 ° C for 2 hours to activate the micropores (specific surface area increased to 220 m 2 / g); when mixing, vermiculite and diatomaceous earth were first dry-mixed for 10 minutes, and then the phase change microcapsules were added and stirred at a low speed; the resulting matrix was tested in a -1°C environment and showed: the temperature fluctuated by 0.18°C and the latent heat of phase change reached 142J / g.

[0022] (4) In the first stage, a cold storage environment is induced and a negative oxygen ion generator is configured to maintain an air negative ion concentration of 800-1200 / cm 3 , and periodically input sound wave stimulation with a frequency of 28kHz, three times a day, 20 minutes each time, with the sound intensity controlled below 55dB; during the second stage of physiological activation, atomized nutrient solution containing 5-aminolevulinic acid was injected simultaneously, with a concentration of 0.1mmol / L, once every 48 hours. During the first stage of cold storage: a negative oxygen ion generator (voltage 12kV) was installed on the top of the storage body to maintain an ion concentration of 1000 / cm 3Piezoelectric ceramic acoustic wave transmitters (frequency 28kHz ± 0.5kHz) were placed along the cultivation racks, emitting 55dB acoustic waves for 20 minutes daily at 9:00 AM, 2:00 PM, and 7:00 PM. During the second activation phase, an ultrasonic atomizer was used to spray a nutrient solution containing 5-ALA (5-aminolevulinic acid) (concentration 0.1 mmol / L, pH 6.8) every 48 hours, with a droplet diameter of ≤10 μm and a deposition rate of 0.8 mL per plant.

[0023] (5) The gradient recovery treatment adopts a zoned temperature control strategy, dividing the transition environment into three temperature zones: the first temperature zone is 3.5℃±0.5℃, humidity is 80±5%, and stays for 24 hours; the second temperature zone is 5℃±0.5℃, humidity is 75±3%, and stays for 48 hours; the third temperature zone is 7℃±1℃, humidity is 68±2%, and stays for 72 hours; the air flow rate in each temperature zone is maintained at a directional circulation of 0.1-0.3m / s, among which, the gradient recovery is carried out in three zones: the first zone (3.5℃) is equipped with an ultrasonic humidifier to maintain humidity at 80%, air flow rate is 0.2m / s, and stays for 24 hours; the second zone (5℃) uses a centrifugal dehumidifier to control humidity at 75%, air flow organization is vertical laminar flow (0.25m / s), and stays for 48 hours; the third zone (7℃) is equipped with humidity at 68%, infrared radiation panels are configured to compensate for heat loss, and stays for 72 hours. When transferring between zones, the seedlings stay in the buffer zone (temperature difference ≤1℃) for 30 minutes to adapt.

[0024] (6) 24 hours before shipment, stress-resistant strengthening treatment was implemented, including spraying a compound solution containing 0.05% betaine and 0.1% silicon potassium minerals on the leaves, pouring a pH 6.5 phosphate buffer into the roots, and applying 14 hours of red light irradiation with a wavelength of 660nm to activate the light protection mechanism. Among them, 24 hours before shipment: spraying a compound solution containing betaine (0.05wt%) + potassium silicate (0.1wt%) on the leaves (atomization pressure 0.3MPa, coverage ≥95%); pouring a pH 6.5 phosphate buffer into the roots (50mL / plant); irradiating the roots with a 660nm red light array (intensity 25μmol·m -2 ·s -1 ) for 14 hours continuously, the spot uniformity is >85%.

[0025] (7) A special refrigeration system, comprising: an insulated storage body with a bionic sand surface texture, the inner wall of which is provided with a micro-topography groove structure with a depth of 0.8±0.3mm; a programmable temperature control system to achieve precise control of ±0.1℃; a rotating cultivation rack with an adjustable tilt angle of 5-12°, which completes a 360° uniform rotation every 120 minutes; a root ball fixing device equipped with a dual-mode monitoring unit, in which a near-infrared spectral probe detects changes in root moisture content in real time, and an electrical impedance sensor dynamically tracks cell membrane integrity; a spectrally adjustable light source system provides continuously adjustable light with a wavelength of 400-700nm, and a built-in sunrise simulation timing controller, wherein , a special system is built: the warehouse body adopts polyurethane sandwich panel (thickness 150mm) with internal die-cast bionic sand dune texture mold (groove depth 0.8mm, spacing 15mm); the temperature control system is equipped with a PID adjustment refrigeration unit (±0.1℃ accuracy); the cultivation rack is installed at a 5° inclination angle and driven by a stepper motor to rotate one circle every 120 minutes; the root ball holder has a built-in near-infrared sensor (light source 1450 / 1940nm dual wavelength) and a four-electrode electrical impedance probe (frequency 10kHz-1MHz); the light source system includes an LED matrix (400-700nm adjustable) and an astronomical clock controller to automatically match the sunrise parameters of the seedling origin.

[0026] (8) The rotating cultivation rack is equipped with a gravity balance compensation mechanism, which automatically adjusts the position of the counterweight block to maintain dynamic stability when the speed changes; the surface of the cultivation rack is covered with an elastic base pad with a shape memory function, and an array of air permeable holes with a diameter of 2-5 mm is distributed on the surface. The hole density increases with the increase of temperature, and the porosity reaches 35% at 5°C. Among them, the rotating rack is equipped with a counterweight slide rail mechanism: when the speed exceeds 2° / s, the counterweight block automatically slides along the radial direction (displacement ΔL=0.02ω 2 , ω is the angular velocity) to offset the centrifugal force; the base pad is made of shape memory polyurethane (glass transition temperature Tg=3℃), and the surface is laser punched to form a density gradient zone: the porosity is 28% at low temperature (<5℃), and when the temperature is raised to 10℃, the molecular chain stretches to increase the porosity to 42%.

[0027] (9) The dual-mode monitoring unit integrates an artificial intelligence analysis module, which uses a machine learning algorithm to associate the near-infrared absorption peak position (1450nm and 1940nm) with the electrical impedance phase angle data to establish a seedling vitality prediction model. When the predicted germination potential is lower than the threshold, the supplementary processing program is automatically triggered. The monitoring system operates as follows: the near-infrared probe collects the absorbance of the root system at 1450nm (characteristic absorption) and 1940nm (combined frequency) every 30 minutes; the electrical impedance module synchronously measures the phase angle θ (10kHz / 100kHz dual frequency); the data is input into the CNN convolutional neural network (the training set contains 500 sets of physiological data of Haloxylon ammodendron seedlings), and when the output layer activation value y is less than 0.85, an alarm is triggered, and the above-mentioned enhanced processing program is automatically executed.

[0028] (10) The outer layer of the thermal insulation warehouse is made of a composite phase change insulation board with paraffin / graphene composite phase change material embedded inside. The phase change range is -5°C to 10°C, and the thermal inertia index D value is ≥8.0. The warehouse door is equipped with a double-channel airtight locking device, and the door gap is embedded with a magnetic fluid sealing strip. The cold leakage rate is ≤0.05W / m·K. Among them, the insulation layer of the warehouse body is a paraffin / expanded graphite composite phase change board (thickness 30mm, phase change range -5-10°C), and the thermal inertia index D value is 8.3; the double-channel door sealing structure: the first magnetic rubber strip (compression volume 35%), the second inflatable fluororubber tube (pressure 0.15MPa); the door gap detection shows that the cold leakage rate is 0.048W / m·K, which meets the ultra-low temperature refrigerator sealing standard.

[0029] Example 2: Multi-source data joint modeling scenario Scenario name: 150-day delayed germination treatment of Haloxylon ammodendron seedlings 1. Root ball preparation and matrix filling On December 25th (during deep dormancy), healthy seedlings with a ground diameter of 1.8 ± 0.2 cm were selected. Using a curved shovel, the taproot was excavated, leaving a length of 45 ± 5 cm, to form a root ball with a diameter of 32 ± 3 cm (96% retention of the original sandy soil). A sodium alginate-montmorillonite composite gel (7:3 mass ratio) was sprayed onto the root ball surface to form a 0.5 mm breathable film. This film was then wrapped with 80 g / m2 hemp fiber non-woven fabric and placed in a 35 cm diameter humidity-controlled container. The container was then filled with a premixed cool storage matrix consisting of 6 kg of vermiculite (3-5 mm particle size), 3 kg of activated diatomaceous earth (calcined at 300°C for 2 hours), and 1 kg of nano-octacosanoic acid phase change microcapsules (0.8 mm particle size). The moisture content was adjusted to 10.2 ± 0.5%.

[0030] 2. First stage induced cold storage Place the container in a bionic reservoir at -0.5±0.1℃ (with an inner wall groove depth of 0.8mm) and turn on the negative oxygen ion generator to maintain a concentration of 950±50 / cm 3 The refrigerated environment was kept dark throughout the entire process, with the CO2 concentration controlled at 980±20ppm and the relative humidity at 90±2%. The piezoelectric ceramic transmitter was activated at 10:00, 15:00, and 20:00 daily, applying 28kHz / 55dB acoustic stimulation for 20 minutes each time. After 45 days of continuous testing, the root electrolyte exudation rate was 12.3% (compared to 38.7% in the control group) and the mycorrhizal fungal survival rate was 91.5%.

[0031] 3. Second stage physiological activation Move to the low light area of ​​0.8±0.2℃, and rotate the cultivation rack once every 120 minutes at an 8° inclination angle (angular velocity ω=0.0087rad / s). The counterweight is displaced according to the formula ΔL=0.02ω. 2Automatic balance. Turn on the 460nm blue light LED (intensity 12.5μmol·m -2 ·s -1 , uniformity >90%). A 0.1 mmol / L 5-ALA solution (particle size 8 μm, sedimentation volume 0.8 mL / plant) was sprayed every 48 hours. The Fv / Fm value increased to 0.78 on the 10th day of activation.

[0032] 4. Partitioned Gradient Recovery Gradual treatment in three temperature zones: Zone 1 (3.5°C): Humidity 80%, vertical airflow 0.2m / s, stay for 24 hours; Zone 2 (5.0°C): Humidity 75%, laminar wind speed 0.25m / s, stay 48 hours; Zone 3 (7.0°C): Humidity 68%, infrared compensation heat flux 15W / ㎡, stay 72 hours; During inter-regional transfer, the cells were adapted to the temperature difference buffer zone (ΔT≤1°C) for 30 minutes, and monitoring showed that the membrane lipid unsaturation increased from 35.2% to 48.6%.

[0033] 5. Stress resistance strengthening and delivery 24 hours before shipment: Spray 0.05% betaine + 0.1% potassium silicate compound solution on the leaves (coverage rate 97%); The roots were perfused with pH 6.5 phosphate buffer (50 mL / plant); Turn on 660nm red light (intensity 25μmol·m -2 ·s -1 ) Continuous irradiation for 14 hours; Real-time monitoring system shows: near infrared 1450nm absorption value A 1450 =0.82, 1940nm absorption value A 1940 =0.75, the impedance phase angle θ=68.5°. Calculated by the CNN vitality model (input layer [A 1450 , A 1940 , θ], output layer y=0.92>threshold 0.85), it is determined that the outbound standard is met.

[0034] 6. Planting Verification Indicators Invention Group Traditional Group Seedling acclimatization period: 5.2 days to 14.7 days New root production: 18.3 6.4 90-day survival rate 93.7% 67.2% The germination time deviated from the predicted value by 2.3 days.

[0035] Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense, and may refer to mechanical or electrical connections, internal communication between two components, or direct connection. "Up," "down," "left," and "right" are only used to indicate relative positional relationships. When the absolute positions of the objects being described change, the relative positional relationships may also change. Secondly: The drawings of the embodiments disclosed in the present invention only involve structures related to the embodiments disclosed in the present invention. Other structures may refer to conventional designs. The same embodiment and different embodiments of the present invention may be combined with each other without conflict. Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A technology for delaying germination of desert seedlings by cold storage, characterized in that The method comprises the following consecutive steps: excavating a psammophyte seedling in a deep dormancy period together with the original sandy soil at the root to form a spherical root ball, wherein the diameter of the root ball is controlled within the range of 18±2 times the diameter of the seedling at ground level, and the original sandy soil retention is ≥95%; placing the spherical root ball in a humidity-controlled container filled with a cold storage matrix, wherein the cold storage matrix is ​​composed of vermiculite, diatomaceous earth, and a nano-organic phase change material with a phase change temperature of -0.8°C, mixed in a mass ratio of 6:3:1, and the moisture content is maintained at a constant level of 10±2%; placing the container containing the root ball in a dark, sealed environment at a temperature of -1°C to 0.5°C for a first stage of induced cold storage for 40±5 days, during which the carbon dioxide concentration is maintained at 1000±200ppm and the relative humidity is maintained at 88±2%; and then transferring the container to a low-light environment at a temperature of -0.3°C to 1°C for a second stage of physiological activation, wherein light with a wavelength of 460±10nm and an intensity of 12±3μmol·m -2 ·s -1 The plants were irradiated with blue light, and the irradiation period was synchronized with the sunrise time in the original habitat of the seedlings; finally, gradient recovery treatment was carried out in a transitional environment of 3.5℃±1.5℃, with a daily heating rate not exceeding 0.3℃ / hour, and the relative humidity was linearly reduced from 85% to 68%, which lasted for 6±1 days.

2. The method for refrigerating and delaying germination of desert seedlings according to claim 1, wherein: The process of forming the spherical root ball includes retaining the main root system length of not less than 40 cm during excavation, controlling the lateral root damage rate below 5%, wrapping the root ball with degradable non-woven fabric and using plant adhesive to fix the sand structure. The adhesive component is a composite gel of sodium alginate and montmorillonite in a ratio of 7:

3. After curing, the oxygen permeability reaches more than 90%.

3. The method for refrigerating and delaying germination of desert seedlings according to claim 1, wherein: The preparation of the cold storage matrix includes encapsulating a nano-organic phase change material in a porous silica microcapsule to form slow-release particles with a particle size of 0.5-1 mm. The diatomaceous earth is heat-treated at 300°C to activate the microporous structure. After mixing, the cold storage capacity of the matrix at a -1°C environment is 3.2 times that of conventional sand, and the temperature fluctuation range is ≤±0.2°C.

4. The method for refrigerating and delaying germination of desert seedlings according to claim 1, wherein: The first stage induces a cold storage environment and configures a negative oxygen ion generator to maintain an air negative ion concentration of 800-1200 / cm 3 , and periodically input sound wave stimulation with a frequency of 28kHz, 3 times a day, 20 minutes each time, and the sound intensity is controlled below 55dB; during the second stage of physiological activation, atomized nutrient solution containing 5-aminolevulinic acid is injected simultaneously, with a concentration of 0.1mmol / L, once every 48 hours.

5. The method for refrigerating and delaying germination of desert seedlings according to claim 1, characterized in that: The gradient resuscitation treatment adopts a zoned temperature control strategy, dividing the transition environment into three temperature zones: the first temperature zone is 3.5℃±0.5℃, humidity is 80±5%, and stays for 24 hours; the second temperature zone is 5℃±0.5℃, humidity is 75±3%, and stays for 48 hours; the third temperature zone is 7℃±1℃, humidity is 68±2%, and stays for 72 hours; the air flow rate in each temperature zone is maintained at a directional circulation of 0.1-0.3m / s.

6. The method for delaying germination of desert seedlings by cold storage according to claim 1, characterized in that: 24 hours before shipment, stress resistance strengthening treatment was implemented, including spraying a compound solution containing 0.05% betaine and 0.1% silicon potassium minerals on the leaves, perfusing the roots with pH 6.5 phosphate buffer, and applying 14 hours of red light irradiation with a wavelength of 660nm to activate the light protection mechanism.

7. A special refrigeration system for implementing the method according to claims 1-6, characterized in that include: The insulated storage body has a bionic sand surface texture, and its inner wall is equipped with a micro-topography groove structure with a depth of 0.8±0.3mm; the programmable temperature control system can achieve precise regulation of ±0.1℃; the rotating cultivation rack with an adjustable tilt angle of 5-12° completes 360° uniform rotation every 120 minutes; the root ball fixing device is equipped with a dual-mode monitoring unit, in which the near-infrared spectrum probe detects changes in root moisture content in real time, and the electrical impedance sensor dynamically tracks cell membrane integrity; the spectrally tunable light source system provides continuously adjustable light with a wavelength of 400-700nm, and has a built-in sunrise simulation timing controller.

8. The dedicated refrigeration system according to claim 7, characterized in that: The rotating cultivation rack is equipped with a gravity balance compensation mechanism, which automatically adjusts the position of the counterweight block to maintain dynamic stability when the speed changes; the surface of the cultivation rack is covered with an elastic base pad with shape memory function, and an array of air permeable holes with a diameter of 2-5mm is distributed on the surface. The hole density increases with increasing temperature, and the porosity reaches 35% at 5°C.

9. The dedicated refrigeration system according to claim 7, characterized in that: The dual-mode monitoring unit integrates an artificial intelligence analysis module, which uses a machine learning algorithm to associate near-infrared absorption peaks (1450nm and 1940nm) with electrical impedance phase angle data to establish a seedling vitality prediction model, and automatically triggers a supplementary processing program when the predicted germination potential is lower than the threshold.

10. The dedicated refrigeration system according to claim 7, characterized in that: The outer layer of the insulated warehouse body is made of composite phase change insulation board, which is embedded with paraffin / graphene composite phase change material, with a phase change range of -5°C to 10°C and a thermal inertia index D value ≥8.0; the warehouse door is equipped with a double-channel airtight locking device, and the door gap is embedded with a magnetic fluid sealing strip, with a cooling leakage rate ≤0.05W / m·K.

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