Biological compound preparation for preventing and treating nematodes as well as preparation method and application of biological compound preparation

Abstract

The invention provides a biological compound preparation for preventing and controlling nematodes as well as a preparation method and application of the biological compound preparation. The biological compound preparation is prepared from the following components in parts by weight: 50 to 90 parts of oligosaccharide extracting solution, 15 to 35 parts of first plant extract and 15 to 35 parts of second plant extract, the first plant extract comprises any one or a combination of at least two of a pyrethrum extract, a garlic extract, a turmeric extract or a lavender extract; the second plant extract comprises any one or a combination of at least two of a marigold extract, a mugwort extract, a derris extract or a mint extract. The biological compound preparation prepared by utilizing the principle of natural biological defense lines in the nature has an excellent nematode control effect, the components are safe and pollution-free, and drug resistance is not generated; meanwhile, required nutritional ingredients are further provided for the plants, the growth of the plants is promoted, and the disease resistance of the plants is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and in particular relates to a biological composite preparation for preventing and controlling nematodes, and a preparation method and application thereof. Background Art

[0002] Plant nematode diseases are caused by the invasion and parasitism of plant parasites. The nematodes absorb nutrients from the host plants, affecting their normal growth and development. The nematodes' metabolic secretions can also irritate the host plant's cells and tissues, causing plant deformities. Control efforts using natural enemies can also reduce agricultural yields and quality. There are over 2,000 known nematode species, causing losses of over 10% of crops, including grain, fruits and vegetables, timber, and flowers, resulting in incalculable economic losses.

[0003] CN117441723A discloses a botanical composition for controlling crop nematode diseases and its use. The active ingredients are active ingredient A and active ingredient B. Active ingredient A is α-terthiophene, and active ingredient B is selected from one or more of cinnamon oil, garlic oil, carvone, geranyl aldehyde, furfural, and palm oil. This composition significantly enhances the nematicidal activity of thiazolyl and abamectin, providing a synergistic, convenient, safe, and highly effective formulation and application method for improving plant nematode control for farmers.

[0004] CN119744869A discloses a nematode-killing composition and a method for controlling nematodes. By compounding trifluoperazine and cyclofenac, the effect of nematode control can be significantly improved, especially with high activity against pine wood nematodes, providing a new option for pine wood nematode control.

[0005] Currently, there are only a limited number of available nematode control agents, most of which are chemical. Long-term use can lead to varying degrees of resistance. Furthermore, insecticide droplets can bounce off plant surfaces, leading to the loss of active ingredients, reducing control effectiveness and further exacerbating environmental pollution. Therefore, there is an urgent need to develop safe biological nematode control agents. Summary of the Invention

[0006] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a biological composite preparation for controlling nematodes, its preparation method and application. The biological composite preparation prepared by the present invention has excellent nematode control effect, its ingredients are safe and pollution-free, it will not produce drug resistance, further promotes plant growth, and improves the plant's disease resistance.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a biological composite preparation for controlling nematodes, wherein the biological composite preparation comprises, by weight, 50-90 parts of an oligosaccharide extract, 15-35 parts of a first plant extract, and 15-35 parts of a second plant extract;

[0009] The first plant extract comprises any one of pyrethrum extract, garlic extract, turmeric extract or lavender extract, or a combination of at least two thereof;

[0010] The second plant extract includes any one of marigold extract, mugwort extract, derris vine extract or mint extract, or a combination of at least two of them.

[0011] The weight proportions of the oligosaccharide extract can be 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts or 90 parts.

[0012] The weight proportions of the first plant extract may be 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts or 35 parts, etc.

[0013] The weight proportions of the second plant extract may be 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts or 35 parts, etc.

[0014] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0015] The present invention utilizes the principle of biological defense lines naturally existing in nature to combine plant oligosaccharide extracts and natural plant extracts to prepare a biological composite preparation, which has excellent nematode control effects, is safe and pollution-free, and will not produce drug resistance. While the present invention effectively and pollution-free controls nematodes, it further provides plants with necessary nutrients, promotes plant growth, and improves plant disease resistance. The oligosaccharide extract, the first plant extract, and the second plant extract in the present invention have a synergistic effect in controlling nematodes and promoting plant growth.

[0016] Preferably, the oligosaccharide extract is prepared by a method comprising the following steps:

[0017] (1) mixing a plant material and an organic acid aqueous solution, performing extraction, performing solid-liquid separation, and collecting the liquid; the plant material comprises any one or a combination of at least two of sugarcane, bamboo, rice straw, wheat straw, corn stalks, or reeds;

[0018] (2) adding water to adjust the organic acid concentration; precipitating a portion of lignin, performing solid-liquid separation, and collecting the liquid to obtain the oligosaccharide extract.

[0019] The present invention adopts a specific oligosaccharide extract preparation method, which can retain the oligosaccharide effective ingredients in plants that can prevent and control nematodes to the greatest extent. The prepared oligosaccharide extract contains trace lignin, which cooperates with multiple active ingredients to further enhance the nematode prevention and control effect.

[0020] Preferably, in step (1), the mass ratio of the plant raw material to the organic acid aqueous solution is 1:(4-12), for example, it can be 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11 or 1:12, etc.; the mass percentage of the organic acid in the organic acid aqueous solution is 75-95%, for example, it can be 75%, 78%, 80%, 82%, 85%, 88%, 90%, 92% or 95%, etc.

[0021] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0022] Preferably, the organic acid comprises any one of acetic acid, lactic acid, succinic acid, formic acid, propionic acid, salicylic acid or quinic acid, or a combination of at least two thereof.

[0023] Preferably, the organic acid comprises acetic acid, lactic acid and succinic acid.

[0024] The invention adopts an aqueous solution of acetic acid, lactic acid and succinic acid for extraction, and the prepared oligosaccharide chain is moderate, which has a better nematode control effect. Acetic acid, lactic acid and succinic acid have a synergistic effect, can better control the reaction process, and retain the effective ingredients for controlling nematodes.

[0025] Preferably, the extraction temperature is 130-160°C, for example, it can be 130°C, 135°C, 140°C, 145°C, 150°C, 155°C or 160°C; the extraction time is 2.5-6h, for example, it can be 2.5h, 3h, 3.5h, 4h, 4.5h, 5h, 5.5h or 6h; the absolute pressure is 0.3-0.8MPa, for example, it can be 0.3MPa, 0.4MPa, 0.5MPa, 0.6MPa, 0.7MPa or 0.8MPa.

[0026] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0027] Under the reaction conditions of specific extraction temperature, time and absolute pressure, the present invention can better control the reaction process of oligosaccharide extract preparation and retain the effective ingredients for preventing and controlling nematodes.

[0028] Preferably, the step of concentrating the liquid is further included after the liquid is collected.

[0029] Preferably, the concentration specifically includes concentrating the liquid to a solid content of 25-50%, for example, 25%, 28%, 30%, 32%, 35%, 38%, 40%, 42%, 45%, 48% or 50%.

[0030] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0031] Preferably, in step (2), the mass percentage of the organic acid in the solution is 15-25%, for example, it can be 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24% or 25%, etc.

[0032] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0033] The present invention adjusts the organic acid concentration within a specific range by adding water, thereby precipitating part of the lignin, controlling the trace lignin in the oligosaccharide extract, and controlling the lignin content within an effective range for enhancing the defense effect.

[0034] Preferably, the step of concentrating the liquid is further included after the liquid is collected.

[0035] Preferably, the concentration specifically includes concentrating the liquid to a solid content of 5-20%, for example, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19% or 20%, etc.

[0036] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0037] Preferably, the first plant extract or the second plant extract is prepared by a method comprising the following steps:

[0038] The plant raw materials and the extraction reagent are mixed, homogenized and broken, the solid and liquid are separated, and the liquid is collected to obtain the first plant extract or the second plant extract; the plant raw materials include any one of pyrethrum, garlic, turmeric, lavender, marigold, artemisia, derris or mint, or a combination of at least two of them.

[0039] The plant extracts in the present invention can be prepared separately or the raw materials can be mixed and prepared simultaneously, which does not affect the efficacy of the plant extracts in the present invention.

[0040] Preferably, the mass ratio of the plant raw material to the extraction reagent is 1:(5-10), for example, it can be 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5 or 1:10, etc.

[0041] Preferably, the extraction reagent is an ethanol aqueous solution with a volume fraction of 20-90% (for example, 20%, 30%, 40%, 50%, 60%, 70%, 80% or 90%, etc.).

[0042] Preferably, the homogenizing wall breaking pressure is 200-800 bar, for example, 200 bar, 300 bar, 400 bar, 500 bar, 600 bar, 700 bar or 800 bar.

[0043] Preferably, the homogenization wall breaking is performed 2-5 times, for example, 2, 3, 4 or 5 times.

[0044] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0045] Preferably, the first plant extract comprises pyrethrum extract, garlic extract and turmeric extract.

[0046] The pyrethrum extract, garlic extract and turmeric extract in the invention have synergistic effects in preventing and controlling nematodes and promoting plant growth.

[0047] Preferably, the second plant extract comprises marigold extract, artemisia annua extract and derris vine extract.

[0048] The marigold extract, mugwort extract and derris vine extract in the present invention have synergistic effects in preventing and controlling nematodes and promoting plant growth.

[0049] In a second aspect, the present invention provides use of the biological composite formulation described in the first aspect in controlling nematodes and / or promoting plant growth.

[0050] Preferably, the nematode comprises any one or a combination of at least two of root-knot nematodes, pine wood nematodes, soybean cyst nematodes or stem nematodes.

[0051] In a third aspect, the present invention provides a method for controlling nematodes and / or promoting plant growth, comprising applying the biological composite formulation described in the first aspect to plants and / or their cultivation media.

[0052] Preferably, the application method includes any one of foliar spraying, soil mixing, root irrigation, seed soaking, furrow application or hole application, or a combination of at least two of them.

[0053] Preferably, the nematode comprises any one or a combination of at least two of root-knot nematodes, pine wood nematodes, soybean cyst nematodes or stem nematodes.

[0054] Compared with the prior art, the present invention has the following beneficial effects:

[0055] The present invention combines a plant oligosaccharide extract and a natural plant extract, and utilizes the biological defense principle naturally existing in nature to prepare a biological composite preparation that has excellent nematode control effects, is safe and pollution-free, and will not produce drug resistance; at the same time, it further provides plants with necessary nutrients, promotes plant growth, and improves plant disease resistance; the oligosaccharide extract, the first plant extract, and the second plant extract have a synergistic effect in controlling nematodes and promoting plant growth. DETAILED DESCRIPTION

[0056] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0057] If no specific techniques or conditions are specified in the examples, the experiments were carried out according to the techniques or conditions described in the literature in the field or according to the product instructions. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0058] Preparation Example 1

[0059] This preparation example provides an oligosaccharide extract, which is prepared by a method comprising the following steps:

[0060] (1) After crushing the sugarcane raw material, the mixture was mixed with 85% acetic acid aqueous solution, 85% lactic acid aqueous solution, and 85% succinic acid aqueous solution in a mass ratio of 1:3:3:2, and extracted at 145° C. for 4 hours under an absolute pressure of 0.5 MPa; the liquid was collected by filtration, and the liquid was concentrated to a solid content of 35%;

[0061] (2) adding water to adjust the mass percentage of the total organic acid in the solution to 20%, precipitating part of the lignin, filtering, collecting the liquid, and concentrating the obtained liquid product to a solid content of 15% to obtain the oligosaccharide extract.

[0062] Preparation Example 2

[0063] This preparation example provides an oligosaccharide extract, which is prepared by a method comprising the following steps:

[0064] (1) The bamboo raw material was crushed, mixed with 75% acetic acid aqueous solution, 75% lactic acid aqueous solution and 75% succinic acid aqueous solution in a mass ratio of 1:2:1:1, and extracted at 160° C. under an absolute pressure of 0.8 MPa for 2.5 hours; filtered, the liquid was collected, and the liquid was concentrated to a solid content of 25%;

[0065] (2) adding water to adjust the mass percentage of the total organic acid in the solution to 15%, precipitating part of the lignin, filtering, collecting the liquid, and concentrating the obtained liquid product to a solid content of 20% to obtain the oligosaccharide extract.

[0066] Preparation Example 3

[0067] This preparation example provides an oligosaccharide extract, which is prepared by a method comprising the following steps:

[0068] (1) crushing the rice straw raw material, mixing it with a 95% acetic acid aqueous solution, a 95% lactic acid aqueous solution, and a 95% succinic acid aqueous solution in a mass ratio of 1:4:4:4, and extracting it at 130° C. under an absolute pressure of 0.3 MPa for 6 hours; filtering, collecting the liquid, and concentrating the liquid to a solid content of 50%;

[0069] (2) adding water to adjust the mass percentage of the total organic acid in the solution to 25%, precipitating part of the lignin, filtering, collecting the liquid, and concentrating the obtained liquid product to a solid content of 5% to obtain the oligosaccharide extract.

[0070] Preparation Example 4

[0071] This preparation example provides an oligosaccharide extract, which differs from Preparation Example 1 only in that: in step (1), acetic acid aqueous solution is not added, and the reduced amount is proportionally distributed to lactic acid aqueous solution and succinic acid aqueous solution, and the other steps remain unchanged.

[0072] Preparation Example 5

[0073] This preparation example provides an oligosaccharide extract, which differs from Preparation Example 1 only in that: in step (1), no lactic acid aqueous solution is added, and the reduced amount is proportionally distributed to the acetic acid aqueous solution and the succinic acid aqueous solution, and the other steps remain unchanged.

[0074] Preparation Example 6

[0075] This preparation example provides an oligosaccharide extract, which differs from Preparation Example 1 only in that: in step (1), no succinic acid aqueous solution is added, and the reduced amount is proportionally distributed to the acetic acid aqueous solution and the lactic acid aqueous solution, and the other steps remain unchanged.

[0076] Preparation Example 7

[0077] This preparation example provides an oligosaccharide extract, which differs from Preparation Example 1 only in that: in step (1), the "85% by mass acetic acid aqueous solution, 85% by mass lactic acid aqueous solution, and 85% by mass succinic acid aqueous solution" are replaced by equal amounts of "85% by mass oxalic acid aqueous solution, 85% by mass malic acid aqueous solution, and 85% by mass maleic acid aqueous solution", respectively, and the other steps remain unchanged.

[0078] Preparation Example 8

[0079] This preparation example provides an oligosaccharide extract, which differs from Preparation Example 1 only in that: in step (1), "extraction at 145°C for 4 hours" is replaced by "extraction at 120°C for 6.5 hours", and the other steps remain unchanged.

[0080] Preparation Example 9

[0081] This preparation example provides an oligosaccharide extract, which differs from Preparation Example 1 only in that in step (1), "absolute pressure 0.5 MPa" is replaced by "absolute pressure 1 MPa", and the other steps remain unchanged.

[0082] Preparation Example 10

[0083] This preparation example provides a pyrethrum extract, which is prepared by a method comprising the following steps:

[0084] The pyrethrum raw material was crushed, and then mixed with a 50% ethanol aqueous solution at a mass ratio of 1:8, homogenized and broken three times under a pressure of 500 bar, filtered, and the liquid was collected to obtain the pyrethrum extract.

[0085] Preparation Example 11

[0086] This preparation example provides a garlic extract, which is prepared by a method comprising the following steps:

[0087] The garlic raw material was crushed, mixed with a 20% ethanol aqueous solution at a mass ratio of 1:5, homogenized and broken twice under a pressure of 200 bar, filtered, and the liquid was collected to obtain the garlic extract.

[0088] Preparation Example 12

[0089] This preparation example provides a turmeric extract, which is prepared by a method comprising the following steps:

[0090] The turmeric raw material was crushed, mixed with a 90% ethanol aqueous solution at a mass ratio of 1:10, homogenized and broken for 5 times under a pressure of 800 bar, filtered, and the liquid was collected to obtain the turmeric extract.

[0091] Preparation Example 13

[0092] This preparation example provides a marigold extract, which differs from Preparation Example 10 only in that the "pyrethrum raw material" is replaced with an equal amount of "marigold raw material", and the other steps remain unchanged.

[0093] Preparation Example 14

[0094] This preparation example provides an Artemisia argyi extract, which differs from Preparation Example 10 only in that the "pyrethrum raw material" is replaced with an equal amount of "Artemisia argyi raw material", and the other steps remain unchanged.

[0095] Preparation Example 15

[0096] This preparation example provides a derris vine extract, which differs from Preparation Example 10 only in that the "pyrethrum raw material" is replaced with an equal amount of "derris vine raw material", and the other steps remain unchanged.

[0097] Example 1

[0098] This embodiment provides a biological composite preparation, which includes the following components in parts by weight:

[0099]

[0100] The preparation method comprises the steps of uniformly mixing the above product raw materials.

[0101] Example 2

[0102] This embodiment provides a biological composite preparation, which includes the following components in parts by weight:

[0103]

[0104] The preparation method comprises the steps of uniformly mixing the above product raw materials.

[0105] Example 3

[0106] This embodiment provides a biological composite preparation, which includes the following components in parts by weight:

[0107]

[0108] The preparation method comprises the steps of uniformly mixing the above product raw materials.

[0109] Examples 4-9

[0110] This example provides a biocomposite preparation, which differs from Example 1 only in that the "oligosaccharide extract provided in Preparation Example 1" is replaced with equal parts of the "oligosaccharide extracts provided in Preparation Examples 4-9", and the other raw materials remain unchanged.

[0111] Example 10

[0112] This embodiment provides a biocomposite preparation, which differs from Example 1 only in that the "pyrethrum extract" is not added to the first plant extract, and the reduced amount thereof is proportionally distributed to the garlic extract and the turmeric extract, while the other raw materials remain unchanged.

[0113] Example 11

[0114] This embodiment provides a biocomposite preparation, which differs from Example 1 only in that: "garlic extract" is not added to the first plant extract, and its reduced amount is proportionally distributed to the pyrethrum extract and the turmeric extract, while other raw materials remain unchanged.

[0115] Example 12

[0116] This embodiment provides a biocomposite preparation, which differs from Example 1 only in that no "turmeric extract" is added to the first plant extract, and the reduced amount thereof is proportionally distributed to the pyrethrum extract and the garlic extract, while other raw materials remain unchanged.

[0117] Example 13

[0118] This embodiment provides a biocomposite preparation, which differs from Example 1 only in that no "marigold extract" is added to the second plant extract, and its reduced amount is proportionally distributed to the artemisia extract and the derris extract, while other raw materials remain unchanged.

[0119] Example 14

[0120] This embodiment provides a biocomposite preparation, which differs from Example 1 only in that: "Artemisia argyi extract" is not added to the second plant extract, and its reduced amount is proportionally distributed to marigold extract and derris vine extract, and other raw materials remain unchanged.

[0121] Example 15

[0122] This embodiment provides a biocomposite preparation, which differs from Example 1 only in that: "Derris extract" is not added to the second plant extract, and its reduced amount is proportionally distributed to marigold extract and artemisia extract, and other raw materials remain unchanged.

[0123] Comparative Example 1

[0124] This comparative example provides a biological composite preparation, which differs from Example 1 only in that no oligosaccharide extract is added, and the reduced amount thereof is proportionally distributed to the first plant extract and the second plant extract, while other raw materials remain unchanged.

[0125] Comparative Example 2

[0126] This comparative example provides a biological composite preparation, which differs from Example 1 only in that the first plant extract is not added, and its reduced amount is proportionally distributed to the oligosaccharide extract and the second plant extract, and other raw materials remain unchanged.

[0127] Comparative Example 3

[0128] This comparative example provides a biological composite preparation, which differs from Example 1 only in that the second plant extract is not added, and its reduced amount is proportionally distributed to the oligosaccharide extract and the first plant extract, and other raw materials remain unchanged.

[0129] Test Example 1

[0130] (1) Test process: 200 g of potato, 20 g of glucose, 1.5 g of magnesium sulfate, 3 g of sodium dihydrogen phosphate, 20 g of agar, and 1000 mL of water were mixed and sterilized at 121° C. for 20 min to prepare a PDA culture medium; the biocomposite preparations prepared in Examples 1-15 and Comparative Examples 1-3 were diluted 8-fold with water and applied to the sterilized PDA culture medium; pine wood nematodes (pine wood nematodes were derived from naturally infected larch) were inoculated, and the nematode mortality rate was recorded after 5 min, 15 min, and 30 min;

[0131] (2) Test results: As shown in Table 1, the biocomposite preparation provided by the present invention has an excellent nematode control effect. The oligosaccharide extract, the first plant extract, and the second plant extract have a synergistic effect in this regard. The pyrethrum extract, garlic extract, and turmeric extract in the first plant extract have a synergistic effect in this regard. The marigold extract, artemisia extract, and derris extract in the second plant extract have a synergistic effect in this regard. The specific oligosaccharide extract preparation method of the present invention can retain the oligosaccharide active ingredients in the plant that can control nematodes to the greatest extent. The prepared oligosaccharide extract contains trace amounts of lignin, which synergizes with multiple active ingredients to further enhance the nematode control effect. The control of organic acids, extraction temperature, extraction time, and absolute pressure involved in the extraction will affect the reaction process and affect the retention of the nematode control active ingredients in the extract.

[0132] Table 1

[0133]

[0134]

[0135] Test Example 2

[0136] (1) Test crops: cucumber, variety Zhongnong No. 12;

[0137] (2) Test soil: The soil in the greenhouse where root-knot nematode disease is serious is sandy loam with good fertility;

[0138] (3) Test process: Ridges were formed and the test crops were transplanted. There were 57 plots in total, and the measured area of ​​each plot was 20m 2; Spray 20 liters / hectare of the biological composite preparations prepared in Examples 1-15 and Comparative Examples 1-3, dilute with 7500 kg / hectare of water for irrigation, spray 3 times, and repeat 3 times for each treatment; Control group (CK): no fertilizer is applied, and an equal amount of water is sprayed during the same period. 2 months after the cucumbers were transplanted, 5 random sampling points were taken from each plot, 2 plants were taken from each point, and the entire root system of the cucumber plants was dug out. The degree of damage to the cucumbers was evaluated according to the root-knot nematode grading standard (Table 2), and the disease index and control effect were calculated; 2 months after the cucumbers were transplanted, the plant height was investigated, and the plant height growth rate was calculated; when the cucumbers were picked, the yield of the cucumbers harvested within 30 days was continuously investigated, and the yield increase rate was calculated;

[0139]

[0140] Table 2

[0141] Disease level The degree and evaluation criteria of root knots 0 No root knots, healthy root system 1 There are only a few root knots, which account for less than 10% of the total root system. 3 Root knots are obvious, accounting for 11-25% of the total root system 5 Root knots are particularly obvious, accounting for 26-50% of the total root system 7 There are many root knots, accounting for 51-75% of the total root system 9 There are a lot of root knots, accounting for more than 75% of the whole root system.

[0142] (4) Test results: As shown in Table 3, the biocomposite preparation provided by the present invention has excellent nematode control and plant growth promotion effects. The oligosaccharide extract, the first plant extract, and the second plant extract have a synergistic effect in this regard. The pyrethrum extract, garlic extract, and turmeric extract in the first plant extract have a synergistic effect in this regard. The marigold extract, artemisia extract, and derris extract in the second plant extract have a synergistic effect in this regard. The specific oligosaccharide extract preparation method of the present invention can retain the oligosaccharide active ingredients in plants that can control nematodes to the greatest extent. The prepared oligosaccharide extract contains trace amounts of lignin, which synergizes with multiple active ingredients to further enhance the nematode control and plant growth promotion effects. The control of organic acids, extraction temperature, extraction time, and absolute pressure involved in the extraction will affect the reaction process and affect the retention of the nematode control and plant growth promotion active ingredients in the extract.

[0143] Table 3

[0144]

[0145]

[0146] The present invention uses the above-described embodiments to illustrate a biological composite formulation for controlling nematodes, its preparation method, and its use. However, the present invention is not limited to these embodiments, and implementation of the present invention is not necessarily dependent on these embodiments. Those skilled in the art will appreciate that any improvements to the present invention, equivalent substitutions for various raw materials in the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0147] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0148] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A biological compound preparation for controlling nematodes, characterized in that: The biological composite preparation comprises, by weight, 50-90 parts of oligosaccharide extract, 15-35 parts of the first plant extract, and 15-35 parts of the second plant extract; The first plant extract comprises any one of pyrethrum extract, garlic extract, turmeric extract or lavender extract, or a combination of at least two thereof; The second plant extract includes any one of marigold extract, mugwort extract, derris vine extract or mint extract, or a combination of at least two of them.

2. The biocomposite preparation according to claim 1, characterized in that The oligosaccharide extract is prepared by a method comprising the following steps: (1) mixing a plant material and an organic acid aqueous solution, performing extraction, performing solid-liquid separation, and collecting the liquid; the plant material comprises any one or a combination of at least two of sugarcane, bamboo, rice straw, wheat straw, corn stalks, or reeds; (2) adding water to adjust the organic acid concentration; precipitating a portion of lignin, performing solid-liquid separation, and collecting the liquid to obtain the oligosaccharide extract.

3. The biological composite preparation according to claim 2, characterized in that In step (1), the mass ratio of the plant material to the organic acid aqueous solution is 1:(4-12), and the mass percentage of the organic acid in the organic acid aqueous solution is 75-95%; Preferably, the organic acid comprises any one of acetic acid, lactic acid, succinic acid, formic acid, propionic acid, salicylic acid or quinic acid, or a combination of at least two thereof; Preferably, the organic acid comprises acetic acid, lactic acid and succinic acid; Preferably, the extraction temperature is 130-160°C, the extraction time is 2.5-6 hours, and the absolute pressure is 0.3-0.8 MPa; Preferably, the step of collecting the liquid further includes a concentration step; Preferably, the concentration specifically comprises concentrating the liquid to a solid content of 25-50%.

4. The biological composite preparation according to claim 2, characterized in that In step (2), the mass percentage of the organic acid in the solution is 15-25%; Preferably, the step of collecting the liquid further includes a concentration step; Preferably, the concentration specifically comprises concentrating the liquid to a solid content of 5-20%.

5. The biological composite preparation according to claim 1, characterized in that The first plant extract or the second plant extract is prepared by a method comprising the following steps: The plant raw materials and the extraction reagent are mixed, homogenized and broken, the solid and liquid are separated, and the liquid is collected to obtain the first plant extract or the second plant extract; the plant raw materials include any one of pyrethrum, garlic, turmeric, lavender, marigold, artemisia, derris or mint, or a combination of at least two of them.

6. The biological composite preparation according to claim 5, characterized in that The mass ratio of the plant raw material to the extraction reagent is 1:(5-10); Preferably, the extraction reagent is an ethanol aqueous solution with a volume fraction of 20-90%; Preferably, the homogenizing wall breaking pressure is 200-800 bar; Preferably, the homogenization wall breaking is performed 2-5 times.

7. The biocomposite preparation according to any one of claims 1 to 6, characterized in that The first plant extract includes pyrethrum extract, garlic extract and turmeric extract; Preferably, the second plant extract comprises marigold extract, artemisia annua extract and derris vine extract.

8. Use of the biocomposite formulation according to any one of claims 1 to 7 in controlling nematodes and / or promoting plant growth; Preferably, the nematode comprises any one or a combination of at least two of root-knot nematodes, pine wood nematodes, soybean cyst nematodes or stem nematodes.

9. A method for controlling nematodes and / or promoting plant growth, characterized in that: The biocomposite formulation according to any one of claims 1 to 7 is applied to plants and / or their cultivation media.

10. The method according to claim 9, characterized in that The application method includes any one or a combination of at least two of foliar spraying, soil mixing, root irrigation, seed soaking, furrow application or hole application; Preferably, the nematode comprises any one or a combination of at least two of root-knot nematodes, pine wood nematodes, soybean cyst nematodes or stem nematodes.

Images