Oral liquid containing collagen peptide as well as preparation method and application of oral liquid

Abstract

The invention belongs to the technical field of oral liquid, and particularly relates to oral liquid containing collagen peptide as well as a preparation method and application of the oral liquid. The oral liquid comprises the following components in percentage by mass: 10%-30% of collagen peptide, 0.5%-1.2% of an astaxanthin-ascorbic acid compound, 0.05%-0.3% of sodium hyaluronate, 0.1%-0.5% of gamma-aminobutyric acid, 3%-8% of erythritol, 0.05%-1% of a stabilizer, 0.1%-6.0% of concentrated fruit juice or fruit powder, 0.05%-2.0% of food essence and the balance of water. The structural formula of the astaxanthin-ascorbic acid compound is # imgabs0 #. The astaxanthin and the ascorbic acid are chemically combined, so that the water solubility of the astaxanthin is improved, the stability of the ascorbic acid is also improved, and the oral liquid is matched with the collagen peptide, so that the oral liquid has good stability and taste flavor, and the facial skin elasticity can be improved after the oral liquid is taken for a long time.

Description

Technical Field

[0001] The present invention belongs to the technical field of oral liquids, and in particular relates to an oral liquid containing collagen peptides, a preparation method thereof, and an application thereof. Background Art

[0002] With aging, environmental pollution, and radiation from electronic devices, skin health issues become increasingly prominent, especially for the facial skin. As the face ages, collagen and elastic fibers gradually degrade, leading to wrinkles, fine lines, and sagging skin. Furthermore, the accumulation of oxygen free radicals is a significant contributor to skin aging, making antioxidants crucial for combating skin aging.

[0003] Currently, collagen supplementation is a common method for improving skin condition, but traditional collagen has a large molecular weight and is difficult to be fully absorbed by the body. Collagen peptides, as a hydrolysis product of collagen, have a small molecular weight and high biological activity, and can be more effectively absorbed by the body, participating in the synthesis and repair of skin collagen.

[0004] Antioxidants also play a key role in improving facial skin condition. Astaxanthin, a natural antioxidant, possesses strong antioxidant capacity, capable of scavenging harmful substances such as nitrogen dioxide, sulfides, and disulfides, reducing lipid peroxidation, minimizing free radical damage to facial skin cells, delaying skin aging, and improving dryness and roughness. However, its water insolubility limits its use in water-soluble systems, such as water-soluble facial care products and cosmetics. Ascorbic acid (vitamin C) is also an important antioxidant. As a water-soluble vitamin, it not only participates in various biochemical reactions in the body, protecting facial skin cells from oxidative damage, but also promotes collagen synthesis, maintaining skin elasticity and radiance. However, aqueous ascorbic acid solutions are sensitive to light, heat, and oxygen, and are easily decomposed, affecting their effectiveness in improving facial skin condition.

[0005] Although some studies have been devoted to improving the water solubility of astaxanthin or increasing the stability of ascorbic acid, these methods often have some limitations. For example, although physical methods such as microencapsulation and nano-encapsulation can improve the water solubility of astaxanthin to a certain extent, they may affect its antioxidant activity; and means of improving the stability of ascorbic acid, such as adding stabilizers, may introduce other impurities or affect the taste and safety of the product. In addition, single-ingredient products are often difficult to meet people's increasingly diverse skin care needs. For example, antioxidants alone may not be able to fully solve problems such as dry skin and lack of elasticity, and need to be combined with other ingredients to work together.

[0006] In view of the above problems, the present invention chemically combines astaxanthin and ascorbic acid, and introduces collagen peptide components to develop an oral liquid containing collagen peptide. Summary of the Invention

[0007] In order to overcome the shortcomings of the existing technology, the primary purpose of the present invention is to provide an oral liquid containing collagen peptides, which has good stability, taste and flavor. Long-term use can enhance facial skin elasticity and improve facial skin condition.

[0008] Another object of the present invention is to provide a method for preparing the oral liquid containing collagen peptides, which has a simple process.

[0009] The third object of the present invention is to provide an application of the oral liquid containing collagen peptides in the preparation of anti-aging products.

[0010] The above objects of the present invention are achieved through the following technical solutions: An oral liquid containing collagen peptides comprises the following ingredients in percentage by mass: 10-30% collagen peptides, 0.5-1.2% astaxanthin-ascorbic acid complex, 0.05-0.3% sodium hyaluronate, 0.1-0.3% gamma-aminobutyric acid, 3-8% erythritol, 0.05-1.0% stabilizer, 0.1-6.0% concentrated fruit juice or fruit powder, 0.05-2.0% food flavoring, and the balance water. The structural formula of the astaxanthin-ascorbic acid complex is as follows: .

[0011] Furthermore, the preparation process of the astaxanthin-ascorbic acid complex is as follows: (1) Astaxanthin and succinic anhydride are added to dioxane, and then triethylamine is added to react. After the reaction is completed, the reaction solution is purified to obtain intermediate 1; the structural formula of intermediate 1 is: ; (2) Adding intermediate 1, 4-formylphenylboronic acid and triethylamine to acetic anhydride, reacting under heating conditions, and post-treating the reaction solution to obtain intermediate 2; the structural formula of intermediate 2 is: ; (3) Adding the intermediate 2 to a 0.1-0.2 mol / L sodium bicarbonate solution, and then adding ascorbic acid to carry out a stirring reaction. After the reaction is completed, the reaction solution is treated to obtain the astaxanthin-ascorbic acid complex.

[0012] Furthermore, in step (1), the molar ratio of astaxanthin, succinic anhydride and triethylamine is 1:(5-10):(2-4); the reaction temperature is 50-60°C, and the reaction time is 15-20 h.

[0013] Furthermore, in step (2), the molar ratio of the intermediate 1,4-formylphenylboronic acid and triethylamine is 1:(2-3):(2-3); the heating temperature is 85-95° C.; and the reaction time is 5-7 h.

[0014] Furthermore, in step (3), the ratio of the intermediate 2, ascorbic acid and sodium bicarbonate solution is 1 mmol: (3-4) mmol: (10-12) mL; and the reaction time is 18-24 h.

[0015] Furthermore, the collagen peptide is a fish collagen tripeptide; the molecular weight of the collagen peptide is 500-1000 Da; the molecular weight of the sodium hyaluronate is 8-40 wDa; and the stabilizer is selected from at least one of xanthan gum, sodium carboxymethyl cellulose, pectin, gum arabic, locust bean gum, and konjac gum.

[0016] Furthermore, the concentrated juice or fruit powder can be selected from conventional products and can be determined based on market preferences. More preferably, the concentrated juice or fruit powder is selected from at least one of concentrated pomegranate juice, concentrated blueberry juice, concentrated passion fruit juice, concentrated grape juice, concentrated apple juice, concentrated loquat juice, concentrated grapefruit juice, concentrated peach juice, concentrated pear juice, passion fruit powder, blueberry powder, pomegranate powder, grapefruit powder, grape powder, lemon powder, blood orange powder, apple powder, and pineapple powder; and the food flavor is selected from at least one of compound berry flavor, passion fruit flavor, pomegranate flavor, lemon flavor, grape flavor, grapefruit flavor, loquat flavor, pineapple flavor, blueberry flavor, lychee flavor, and peach flavor.

[0017] The method for preparing the oral liquid containing collagen peptides comprises the following steps: (a) Weighing the raw materials according to the mass percentages, adding collagen peptide, erythritol, and sodium hyaluronate to water, and heating to dissolve to obtain a mixed solution 1; (b) Cooling the mixed solution 1 to room temperature, sequentially adding astaxanthin-ascorbic acid complex, γ-aminobutyric acid, and concentrated pomegranate juice, high-pressure homogenizing the mixture, and then adding xanthan gum and sodium carboxymethyl cellulose, and stirring the mixture to obtain a mixed solution 2; (c) Lemon essence is added to the mixed solution 2 and stirred evenly, and the mixture is filtered, bottled, sterilized, and capped to obtain the oral solution containing collagen peptides.

[0018] Furthermore, the heating temperature in step (a) is 60-70° C.; the high-pressure homogenization in step (b) is performed at a pressure of 20-25 MPa, for a time of 1-1.5 min, and for 2-3 times.

[0019] Furthermore, the sterilization temperature in step (c) is 80-90° C. and the time is 10-15 min.

[0020] The oral liquid containing collagen peptides is used to prepare anti-aging products.

[0021] The present invention has the following effects compared to the prior art: 1. The present invention provides an oral liquid containing collagen peptides, comprising collagen peptides, an astaxanthin-ascorbic acid complex, and other ingredients. By chemically combining astaxanthin and ascorbic acid, the present invention improves the water solubility of astaxanthin and the stability of ascorbic acid, enhancing the antioxidant capacity of both. When used in combination with collagen peptides, the resulting oral liquid exhibits excellent stability, taste, and flavor. Long-term use can enhance facial elasticity and improve facial skin condition.

[0022] 2. The present invention also provides a method for preparing the oral liquid containing collagen peptides, which is simple in process and provides a new idea and method for improving facial skin condition. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 Schematic diagram of the preparation process of the astaxanthin-ascorbic acid complex of the present invention. DETAILED DESCRIPTION

[0024] The technical solution of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. Specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. All reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels. Example 1:

[0025] Embodiment 1: An oral liquid containing collagen peptides is composed of the following ingredients in percentage by mass: 20% fish collagen tripeptide, 0.8% astaxanthin-ascorbic acid complex, 0.15% sodium hyaluronate, 0.25% gamma-aminobutyric acid, 6% erythritol, 0.2% xanthan gum, 0.6% sodium carboxymethyl cellulose, 2% concentrated pomegranate juice, 1% lemon essence, and the balance water; the structural formula of the astaxanthin-ascorbic acid complex is as follows: ; The preparation process of the above-mentioned astaxanthin-ascorbic acid complex is as follows. The reaction process is as follows Figure 1 As shown: (1) Astaxanthin (1 mmol) and succinic anhydride (8 mmol) were added to dioxane (50 mL), and then triethylamine (3 mmol) was added as a catalyst. The reaction was carried out at 55°C for 18 h. After the reaction, 0.1 mol / L hydrochloric acid solution was added to the reaction solution until the precipitation was complete. The precipitate was collected and purified by silica gel column chromatography. The precipitate was gradient eluted with chloroform-methanol (100:0 / 90:10 / 0:100, v / v). The eluates were combined and the solvent was evaporated to obtain intermediate 1. Intermediate 1 1 H NMR( C 48 H 60 O 10 , 400 MHz, d 6 -DMSO) δ 12.16 (s, 2H), 6.51 (d,10H), 6.24 (d, 4H), 5.45 (t, 2H), 2.71 (t, 4H), 2.53 (t, 4H), 2.36 (s, 6H), 2.14 (s, 12H), 1.70-1.68 (m, 2H), 1.46-1.44 (m, 2H), 1.25 (s, 12H); HRMS (ESI + ): [M+H] + The calculated value was 797.42, and the found value was 797.40; the above results confirmed that the obtained product was the target product.

[0026] (2) Add intermediate 1 (10 mmol), 4-formylphenylboronic acid (25 mmol) and triethylamine (25 mmol) to acetic anhydride (55 mL) and react at 90°C for 6 h. After the reaction, cool the reaction solution to room temperature, add diethyl ether (25 mL) and water (20 mL) to precipitate a solid. Filter and collect the organic phase and extract with 12% NaOH aqueous solution (3×10 mL). Combine the aqueous phases and add concentrated hydrochloric acid to pH = 2. Collect the precipitate, wash with water, and dry to obtain intermediate 2. Intermediate 2 1 H NMR( C 62 H 70 B2O 14 , 400 MHz, d 6-DMSO) δ 12.20 (s, 2H), 7.71(d,4H), 7.35(d, 4H), 7.01(s, 2H), 6.51 (d, 10H), 6.24 (d, 4H), 5.45 (t, 2H),4.28 (s, 4H), 3.56 (s, 4H), 2.36 (s, 6H), 2.14 (s, 12H), 1.70-1.68 (m, 2H),1.46-1.44 (m, 2H), 1.25 (s, 12H); HRMS (ESI + ): [M+H] + The calculated value was 1061.50, and the found value was 1061.47; the above results confirmed that the obtained product was the target product.

[0027] (3) Intermediate 2 (10 mmol) was added to a sodium bicarbonate solution (0.15 mol / L, 110 mL), and then ascorbic acid (35 mmol) was added and stirred for 20 h. After the reaction, the reaction solution was purified by column chromatography to obtain the astaxanthin-ascorbic acid complex.

[0028] Astaxanthin-ascorbic acid complex 1 H NMR( C 74 H 76 B2O 22 Na2, 400 MHz, d 6 -DMSO) δ 7.71(d, 4H), 7.35(d, 4H), 7.01(s, 2H), 6.51 (d, 10H), 6.24 (d, 4H), 5.78(s, 2H),5.45 (t, 2H), 4.81 (d, 2H), 4.24-4.22(m, 2H), 3.96(s, 2H), 3.59-3.53(m, 4H),3.06 (s, 4H), 2.36 (s, 6H), 2.14 (s, 12H), 1.70-1.68 (m, 2H), 1.46-1.44 (m,2H), 1.25 (s, 12H); HRMS (ESI + ): [M+H] + The calculated value was 1385.48, and the found value was 1385.52; the above results confirmed that the obtained product was the target product.

[0029] This embodiment also provides a method for preparing the oral liquid containing collagen peptides, and the specific process is as follows: (a) Weighing the raw materials according to the above mass percentages, adding fish collagen tripeptide, erythritol, and sodium hyaluronate to water, and stirring at 65° C. until dissolved to obtain a mixed solution 1; (b) Mixture 1 was cooled to room temperature, and astaxanthin-ascorbic acid complex, γ-aminobutyric acid, and concentrated pomegranate juice were added in sequence. The mixture was homogenized at 22 MPa for 3 times, each time for 1 min. Xanthan gum and sodium carboxymethyl cellulose were then added, and the mixture was stirred for 30 min until homogeneous, thereby obtaining mixture 2. (c) Lemon essence is added to the mixed solution 2 and stirred evenly. After filtering, the mixture is filled into oral liquid bottles, pasteurized at 85° C. for 12 min, cooled, and capped to obtain the oral liquid containing collagen peptides.

[0030] Example 2: An oral liquid containing collagen peptides, comprising the following ingredients in percentage by mass: 10% fish collagen tripeptide, 0.5% astaxanthin-ascorbic acid complex, 0.05% sodium hyaluronate, 0.1% gamma-aminobutyric acid, 3% erythritol, 0.02% gum arabic, 0.03% pectin, 6% concentrated blueberry juice, 0.05% passion fruit essence, and the balance water; the structural formula of the astaxanthin-ascorbic acid complex is the same as that of Example 1; The preparation process of the above-mentioned astaxanthin-ascorbic acid complex is as follows. The reaction process is as follows Figure 1 As shown: (1) Astaxanthin (1 mmol) and succinic anhydride (5 mmol) were added to dioxane (50 mL), and then triethylamine (2 mmol) was added as a catalyst. The reaction was carried out at 50°C for 20 h. After the reaction, 0.1 mol / L hydrochloric acid solution was added to the reaction solution until the precipitation was complete. The precipitate was collected and purified by silica gel column chromatography. The precipitate was gradient eluted with chloroform-methanol (100:0 / 90:10 / 0:100, v / v). The eluates were combined and the solvent was evaporated to obtain intermediate 1. The nuclear magnetic resonance and mass spectrometry results of intermediate 1 were the same as those in Example 1.

[0031] (2) Add intermediate 1 (10 mmol), 4-formylphenylboronic acid (20 mmol) and triethylamine (20 mmol) to acetic anhydride (50 mL) and react at 85°C for 7 h. After the reaction, cool the reaction solution to room temperature, add diethyl ether (25 mL) and water (20 mL) to precipitate a solid. Filter and collect the organic phase and extract with 12% NaOH aqueous solution (3 × 10 mL). Combine the aqueous phases and add concentrated hydrochloric acid to pH = 2. Collect the precipitate, wash with water, and dry to obtain intermediate 2. The NMR and mass spectrometry results of intermediate 2 are the same as those in Example 1.

[0032] (3) The intermediate 2 (10 mmol) was added to a sodium bicarbonate solution (0.1 mol / L, 120 mL), and then ascorbic acid (30 mmol) was added and stirred for 18 h. After the reaction, the reaction solution was purified by column chromatography to obtain the astaxanthin-ascorbic acid complex. The nuclear magnetic resonance and mass spectrometry results of the astaxanthin-ascorbic acid complex were the same as those in Example 1.

[0033] This embodiment also provides a method for preparing the oral liquid containing collagen peptides, and the specific process is as follows: (a) Weighing the raw materials according to the above mass percentages, adding fish collagen tripeptide, erythritol, and sodium hyaluronate to water, and stirring at 60° C. until dissolved to obtain a mixed solution 1; (b) Mixture 1 was cooled to room temperature, and astaxanthin-ascorbic acid complex, γ-aminobutyric acid, and concentrated pomegranate juice were added in sequence. The mixture was homogenized at 20 MPa for three times, each time for 1.5 min. Xanthan gum and sodium carboxymethyl cellulose were then added, and the mixture was stirred for 30 min until homogeneous, thereby obtaining mixture 2. (c) Lemon essence is added to the mixed solution 2 and stirred evenly. After filtering, the mixture is filled into oral liquid bottles, pasteurized at 80° C. for 15 min, cooled, and capped to obtain the oral liquid containing collagen peptides.

[0034] Example 3: An oral liquid containing collagen peptides, comprising the following ingredients in percentage by mass: 30% fish collagen tripeptide, 1.2% astaxanthin-ascorbic acid complex, 0.3% sodium hyaluronate, 0.5% gamma-aminobutyric acid, 8% erythritol, 1.0% locust bean gum, 0.1% passion fruit powder, 2% grape essence, and the balance water; the structural formula of the astaxanthin-ascorbic acid complex is the same as that of Example 1; The preparation process of the above-mentioned astaxanthin-ascorbic acid complex is as follows. The reaction process is as follows Figure 1 As shown: (1) Astaxanthin (1 mmol) and succinic anhydride (10 mmol) were added to dioxane (50 mL), and then triethylamine (4 mmol) was added as a catalyst. The reaction was carried out at 60°C for 15 h. After the reaction, 0.1 mol / L hydrochloric acid solution was added to the reaction solution until the precipitation was complete. The precipitate was collected and purified by silica gel column chromatography. The precipitate was gradient eluted with chloroform-methanol (100:0 / 90:10 / 0:100, v / v). The eluates were combined and the solvent was evaporated to obtain intermediate 1. The NMR and mass spectrometry results of intermediate 1 were the same as those in Example 1.

[0035] (2) Add intermediate 1 (10 mmol), 4-formylphenylboronic acid (30 mmol) and triethylamine (30 mmol) to acetic anhydride (60 mL) and react at 95°C for 5 h. After the reaction, cool the reaction solution to room temperature, add diethyl ether (25 mL) and water (20 mL) to precipitate a solid. Filter and collect the organic phase and extract with 12% NaOH aqueous solution (3 × 10 mL). Combine the aqueous phases and add concentrated hydrochloric acid to pH = 2. Collect the resulting precipitate, wash with water, and dry to obtain intermediate 2. The NMR and mass spectrometry results of intermediate 2 are the same as those in Example 1.

[0036] (3) The intermediate 2 (10 mmol) was added to a sodium bicarbonate solution (0.2 mol / L, 100 mL), and then ascorbic acid (40 mmol) was added and stirred for 24 h. After the reaction, the reaction solution was purified by column chromatography to obtain the astaxanthin-ascorbic acid complex. The nuclear magnetic resonance and mass spectrometry results of the astaxanthin-ascorbic acid complex were the same as those in Example 1.

[0037] This embodiment also provides a method for preparing the oral liquid containing collagen peptides, and the specific process is as follows: (a) Weighing the raw materials according to the above mass percentages, adding fish collagen tripeptide, erythritol, and sodium hyaluronate to water, and stirring at 70° C. until dissolved to obtain a mixed solution 1; (b) Mixture 1 was cooled to room temperature, and astaxanthin-ascorbic acid complex, γ-aminobutyric acid, and concentrated pomegranate juice were added in sequence. The mixture was homogenized at 25 MPa for 1 min each time, and then xanthan gum and sodium carboxymethyl cellulose were added. The mixture was stirred for 30 min until homogeneous, thereby obtaining mixture 2. (c) Lemon essence is added to the mixed solution 2 and stirred evenly. After filtering, the mixture is filled into oral liquid bottles, pasteurized at 90° C. for 10 min, cooled, and capped to obtain the oral liquid containing collagen peptides.

[0038] Comparative Example 1: Comparative Example 1 is substantially the same as Example 1, except that the astaxanthin-ascorbic acid complex in Example 1 is replaced by astaxanthin.

[0039] Comparative Example 2: Comparative Example 2 is substantially the same as Example 1, except that the astaxanthin-ascorbic acid complex in Example 1 is replaced by ascorbic acid.

[0040] Comparative Example 3: Comparative Example 3 is substantially the same as Example 1, except that the astaxanthin-ascorbic acid complex in Example 1 is replaced by a mixture of astaxanthin and ascorbic acid, and the amounts of astaxanthin and ascorbic acid used are the same as those in Example 1.

[0041] Test Example 1 Stability Test of Oral Liquid The oral liquids obtained in Examples 1-3 and Comparative Examples 1-3 were placed at room temperature for two weeks and then observed for stratification or precipitation. Ten volunteers were selected to evaluate the taste and odor of the oral liquids obtained in Examples 1-3 and Comparative Examples 1-3. The results are shown in Table 1. Table 1: ; As can be seen from Table 1, the oral liquids obtained in Examples 1-3 of the present invention have good stability, exhibit no stratification or precipitation after being stored at room temperature for two weeks, and exhibit good flavor and a refreshing mouthfeel. Compared to Example 1, the stability, taste, and odor of Comparative Examples 1-3 are inferior, indicating that the present invention, by chemically combining astaxanthin and ascorbic acid, not only improves the water solubility of astaxanthin but also enhances the stability of ascorbic acid, thereby facilitating full utilization of the antioxidant capacity of both.

[0042] Test Example 2 Antioxidant Activity Test: The oral liquids containing collagen peptides prepared in Examples 1-3 and Comparative Examples 1-3 were tested for antioxidant activity, and the specific method is as follows: Use anhydrous ethanol to prepare a 0.1 mmol / L DPPH solution.

[0043] 100 μL of 0.1 mmol / L DPPH solution was added to a transparent tube, and then 100 μL of anhydrous ethanol solution was added and mixed. The mixture was reacted in the dark for 40 min, and the absorbance of the mixture was measured at 517 nm and recorded as B0. 100 μL of 0.1 mmol / L DPPH solution was added to a transparent tube, and then 100 μL of oral solution from each group was added and mixed. The mixture was reacted in the dark for 40 min, and the absorbance of the mixture was measured at 517 nm, which was recorded as B1. 100 μL of anhydrous ethanol was added to a transparent tube, and then 100 μL of oral solution from each group was added and mixed. The mixture was reacted in the dark for 40 min, and the absorbance of the mixture was measured at 517 nm and recorded as B2. DPPH free radical scavenging rate = {1-[(B1-B2) / B0]}×100%. The results of DPPH free radical scavenging rate are shown in Table 2.

[0044] Table 2: ; As shown in Table 2, the oral liquids of Examples 1-3 have good antioxidant activity. Compared with Example 1, the antioxidant activity of the oral liquids obtained in Comparative Example 1, in which the astaxanthin-ascorbic acid complex is replaced with astaxanthin, the astaxanthin-ascorbic acid complex is replaced with ascorbic acid in Comparative Example 2, and the astaxanthin-ascorbic acid complex is replaced with a mixture of astaxanthin and ascorbic acid in Comparative Example 3, all decreases. This indicates that the present invention can significantly improve the stability of ascorbic acid and the water solubility of astaxanthin by chemically combining astaxanthin and ascorbic acid, thereby fully exerting its antioxidant capacity.

[0045] Test Example 3 Skin elasticity test: 70 female volunteers aged 18-50 with healthy skin and no damage were screened and randomly divided into 7 groups, each with 10 subjects. Among them, 6 groups took the oral liquid corresponding to Examples 1-3 and Comparative Examples 1-3 respectively, and the 7th group took water as a blank control group, once a day, with a dosage of 5g / bottle, 5g per day, taken with warm water before breakfast or dinner. Follow-up was conducted once before use and 2 months after use, and the follow-up was conducted at the same time of day. No other products of the same type were used during the trial. Before the start of the trial, the skin elasticity parameters of the cheeks of the subjects were tested using the Cutometer MPA580 skin elasticity meter. During the measurement, the probe repeated the measurement 3 times in the same tested area, and the average value was taken. After the 7 groups of subjects took the samples for 2 months, the cheeks of the subjects were tested again using the Cutometer MPA580 skin elasticity meter.

[0046] The skin elasticity parameter, R2, is the ratio of the skin's rebound without negative pressure to its maximum stretch with negative pressure. The closer R2 is to 1, the better the skin's elasticity. Based on the R2 parameter, the average R2 change rate was calculated: Average R2 Change Rate (%) = [(R2 value after 2 months of product use / R2 value before product use) / R2 value before product use] × 100%. The test results are shown in Table 3.

[0047] Table 3: ; As can be seen from Table 3, after taking the oral liquid obtained in Examples 1-3, the average change rate of skin R2 is relatively high, and the skin elasticity parameter R2 value of the cheek area increases by more than 17.14%, indicating that the oral liquid of the present invention has the effect of improving the facial skin condition.

[0048] Finally, it should be noted that the above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the same. The basic principles and main features of the present invention have been described above using specific implementation schemes. Modifications or substitutions may be made based on the present invention, but such modifications or substitutions do not detract from the essence of the corresponding technical solutions from the scope of protection claimed by the present invention.

Claims

1. An oral liquid containing collagen peptide, characterized in that: The invention comprises the following ingredients in percentage by mass: 10-30% collagen peptide, 0.5-1.2% astaxanthin-ascorbic acid complex, 0.05-0.3% sodium hyaluronate, 0.1-0.5% gamma-aminobutyric acid, 3-8% erythritol, 0.05-1.0% stabilizer, 0.1-6.0% concentrated fruit juice or fruit powder, 0.05-2.0% food flavoring, and the balance water; the structural formula of the astaxanthin-ascorbic acid complex is as follows: 。 2. The oral liquid containing collagen peptide according to claim 1, characterized in that The preparation process of the astaxanthin-ascorbic acid complex is as follows: (1) Astaxanthin and succinic anhydride are added to dioxane, and then triethylamine is added to react. After the reaction is completed, the reaction solution is purified to obtain intermediate 1; the structural formula of intermediate 1 is: ； (2) Adding intermediate 1, 4-formylphenylboronic acid and triethylamine to acetic anhydride, reacting under heating conditions, and post-treating the reaction solution to obtain intermediate 2; the structural formula of intermediate 2 is: ； (3) Adding the intermediate 2 to a 0.1-0.2 mol / L sodium bicarbonate solution, and then adding ascorbic acid to carry out a stirring reaction. After the reaction is completed, the reaction solution is treated to obtain the astaxanthin-ascorbic acid complex.

3. The oral liquid containing collagen peptide according to claim 2, characterized in that The molar ratio of astaxanthin, succinic anhydride and triethylamine in step (1) is 1: (5-10): (2-4); the reaction temperature is 50-60° C. and the reaction time is 15-20 h.

4. The oral liquid containing collagen peptide according to claim 2, characterized in that In step (2), the molar ratio of the intermediate 1, 4-formylphenylboronic acid and triethylamine is 1:(2-3):(2-3); the heating temperature is 85-95° C.; and the reaction time is 5-7 h.

5. The oral liquid containing collagen peptide according to claim 2, characterized in that In step (3), the ratio of the intermediate 2, ascorbic acid and sodium bicarbonate solution is 1 mmol: (3-4) mmol: (10-12) mL; and the reaction time is 18-24 h.

6. The oral liquid containing collagen peptide according to claim 1, characterized in that The collagen peptide is a fish collagen tripeptide; the molecular weight of the collagen peptide is 500-1000 Da; the molecular weight of the sodium hyaluronate is 8-40 wDa; and the stabilizer is selected from at least one of xanthan gum, sodium carboxymethyl cellulose, pectin, gum arabic, locust bean gum, and konjac gum.

7. The method for preparing the oral liquid containing collagen peptide according to any one of claims 1 to 6, characterized in that: The following steps are involved: (a) Weighing the raw materials according to the mass percentages, adding collagen peptide, erythritol, and sodium hyaluronate to water, and heating to dissolve to obtain a mixed solution 1; (b) Cooling the mixed solution 1 to room temperature, sequentially adding astaxanthin-ascorbic acid complex, γ-aminobutyric acid, and concentrated pomegranate juice, high-pressure homogenizing the mixture, and then adding xanthan gum and sodium carboxymethyl cellulose, and stirring the mixture to obtain a mixed solution 2; (c) Lemon essence is added to the mixed solution 2 and stirred evenly, and the mixture is filtered, bottled, sterilized, and capped to obtain the oral solution containing collagen peptides.

8. The method for preparing an oral liquid containing collagen peptide according to claim 7, characterized in that: The heating temperature in step (a) is 60-70° C.; the high-pressure homogenization in step (b) is performed at a pressure of 20-25 MPa, for a time of 1-1.5 minutes, and 2-3 times.

9. The method for preparing an oral liquid containing collagen peptide according to claim 7, characterized in that: The sterilization temperature in step (c) is 80-90°C and the time is 10-15 minutes.

10. The use of the oral liquid containing collagen peptide according to any one of claims 1 to 6, characterized in that: Used in the preparation of anti-aging products.

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