Preparation technology of collagen surgical suture with antibacterial and anti-inflammatory functions

Abstract

The invention discloses a preparation technology of collagen surgical sutures with antisepsis and anti-inflammation functions, and belongs to the technical field of surgical suture processing, and the preparation technology comprises the following steps: step 1, weighing and cleaning traditional Chinese medicines with antisepsis and anti-inflammation functions in advance, then soaking the traditional Chinese medicines in water for 30 minutes, heating the water to boil, and keeping the temperature for 1 hour; 2, cooling the liquid medicine to 80 DEG C, then filtering, and collecting filtrate; 3, bone glue is added into the 80-DEG C liquid medicine, and heat preservation and stirring are continuously conducted for 1 hour; 4, cooling the liquid medicine to 40 DEG C, and adding vitamin C and vitamin E; according to the preparation technology of the collagen surgical suture with the antibacterial and anti-inflammatory functions, the protein suture'nutritional traditional Chinese medicine coating 'technology does not reduce the breaking force of the suture, but when a wound is healed after an operation, due to the fact that the suture enters liquid medicine in advance, the multiple effects of resisting bacteria, inhibiting bacteria, diminishing inflammation, increasing wound nutrition and the like can be achieved, and the collagen surgical suture is safe and reliable. And the wound infection rate is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of surgical suture processing, and in particular relates to a preparation technology of a collagen surgical suture with antibacterial and anti-inflammatory functions. Background Art

[0002] Using goat casings from southwestern Shandong as raw material, and utilizing unique bio-extraction and processing techniques, this natural absorbable medical surgical (sheep intestine) suture is manufactured. The main components of the suture are collagen and chitosan, making it a pure, natural biological suture material. It is suitable for surface sutures and cosmetic sutures in various surgical procedures. It is fully absorbed by the body, has no toxic side effects, and exhibits excellent tissue compatibility, preventing rejection. Collagenase degrades the suture into amino acids, a nutritious protein that promotes wound healing. It meets all the requirements for an absorbable suture, ensuring rapid recovery. It addresses four major issues with current chemical sutures: chemical residue, potential for inflammation, lack of nutritional value after absorption, and the potential for scar tissue formation. This product offers four key benefits to patients: the absence of chemical residue, resulting in improved health; the absence of inflammation, which reduces pain and burden; the nutritional benefits, which promotes faster recovery; and the absence of scar tissue formation, which provides a beautiful cosmetic effect. It plays an important role in clinical wound suturing and is the development direction of medical sutures. It has a broad market and important clinical application value.

[0003] In clinical surgery, the main factors affecting wound healing include wound infection, nutritional deficiencies, medical device toxicity, and surgical suturing techniques. Nutritional deficiencies are detrimental to wound healing, significantly reducing wound healing, wound tone, and local antimicrobial defenses. Wound nutritional deficiency is a critical issue that needs to be addressed. Current natural protein surgical sutures and synthetic polymer sutures are unable to provide nutrients for wound healing. Summary of the Invention

[0004] The present invention aims to address the technical challenge of biological sutures lacking nutritional properties by adding a "nutritional Chinese medicine coating." This invention pioneers the use of a "nutritional Chinese medicine coating" for sutures. Furthermore, this new technology is used to produce collagen surgical sutures with antibacterial, anti-inflammatory, and nutritional properties. Sutures coated with this "nutritional Chinese medicine coating" exhibit multiple antibacterial, antibacterial, and anti-inflammatory properties, reducing infection rates.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a preparation technology of collagen surgical suture with antibacterial and anti-inflammatory functions, comprising the following steps: Step 1: Weigh and clean the Chinese medicine with antibacterial and anti-inflammatory effects in advance, then soak it in water for 30 minutes, bring the water to a boil, and keep it warm for 1 hour; Step 2: Cool the liquid to 80 degrees, filter it, and collect the filtrate; Step 3: Add bone glue to the 80-degree solution and keep stirring for 1 hour; Step 4: Cool the liquid to 40 degrees and add vitamin C and vitamin E; Step 5: Immerse the tanned collagen surgical suture thread in the drug solution, and load the drug solution onto the collagen surgical suture thread through multiple roller rolling; Step 6. Take out the collagen surgical sutures from the liquid medicine, dry them with cold air, and then sterilize them with high temperature. After completion, inspect them. If they pass the inspection, they will be packaged in a quantitative manner.

[0006] As a preferred embodiment, the Chinese medicine with antibacterial and anti-inflammatory effects in step one includes forsythia, isatis root, honeysuckle, Panax notoginseng, and isatis indigotica, and the weight and mass proportions of each raw material are as follows: forsythia 6-10, isatis root 4-8, honeysuckle 6-10, Panax notoginseng 4-6, isatis indigotica 3-5, and water 100.

[0007] As a preferred embodiment, the bone glue described in step 3 is set to be any one of bovine bone glue, pig bone glue, and sheep bone glue, and the mass proportion of the bone glue is 5 to 10.

[0008] As a preferred embodiment, the mass ratio of vitamin C and vitamin E to the gelatin-containing liquid in step 4 is 0.5-2:0.1-1:100.

[0009] As a preferred embodiment, the immersion time of the collagen surgical suture in the drug solution in step five is 0.5 to 2 minutes, and the mass ratio of the collagen surgical suture is 10 to 50.

[0010] As a preferred embodiment, when the suture thread in step six is ​​dried, it cannot be baked or exposed to direct sunlight, but should be dried naturally in a cool and dry place.

[0011] As a preferred embodiment, the collagen surgical suture that has passed the inspection in step six has a tensile force of more than 16.9N, an increase of 25%, a flexibility increase of more than 10%, an antibacterial rate of 99.9%, a collagen purity of 95%, and a cytotoxicity of less than level 1.

[0012] Compared with the prior art, the technical effects and advantages of the present invention are: The preparation technology of the collagen surgical suture with antibacterial and anti-inflammatory functions and the "nutritional Chinese medicine coating" technology of the protein suture of the present invention do not reduce the breaking force of the suture, but when the wound heals after surgery, the suture can play multiple roles such as antibacterial, antibacterial, anti-inflammatory, and increasing wound nutrition due to the advance entry of the medicine solution, thereby reducing the wound infection rate. DETAILED DESCRIPTION

[0013] In the following description, numerous specific details are provided to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention may be practiced without one or more of these details. In other instances, certain technical features well known in the art are not described to avoid confusion with the present invention. Example

[0014] The present invention provides a preparation technology of collagen surgical suture with antibacterial and anti-inflammatory functions, comprising the following steps: Step 1: Weigh and clean the Chinese medicine with antibacterial and anti-inflammatory effects in advance, then soak it in water for 30 minutes, bring the water to a boil, and keep it warm for 1 hour; Step 2: Cool the liquid to 80 degrees, filter it, and collect the filtrate; Step 3: Add bone glue to the 80-degree solution and keep stirring for 1 hour; Step 4: Cool the liquid to 40 degrees and add vitamin C and vitamin E; Step 5: Immerse the tanned collagen surgical suture thread in the drug solution, and load the drug solution onto the collagen surgical suture thread through multiple roller rolling; Step 6. Take out the collagen surgical sutures from the liquid medicine, dry them with cold air, and then sterilize them with high temperature. After completion, inspect them. If they pass the inspection, they will be packaged in a quantitative manner.

[0015] The Chinese medicines with antibacterial and anti-inflammatory effects in step one include forsythia, isatis root, honeysuckle, Panax notoginseng, and isatis indigotica. The weight and mass proportions of each raw material are as follows: forsythia 6, isatis root 4, honeysuckle 6, Panax notoginseng 4, isatis indigotica 3, and aqueous solution 100.

[0016] The bone glue in step 3 is set to be any one of bovine bone glue, pig bone glue, and sheep bone glue, and the mass percentage of the bone glue is 5.

[0017] The mass ratio of vitamin C and vitamin E to the gelatin-containing solution in step 4 is 0.5:0.1:100.

[0018] The collagen surgical suture thread in step five is immersed in the medicinal solution for 0.5 minutes, and the mass ratio of the collagen surgical suture thread is 10.

[0019] When drying the sutures in step six, do not bake them or expose them to direct sunlight. Instead, dry them naturally in a cool, dry place.

[0020] The collagen surgical suture thread that has passed the inspection in step 6 has a tensile strength of more than 16.9N, an increase of 25%, a flexibility of more than 10%, an antibacterial rate of 99.9%, a collagen purity of 95%, and a cytotoxicity of less than level 1. Example

[0021] The present invention provides a preparation technology of collagen surgical suture with antibacterial and anti-inflammatory functions, comprising the following steps: Step 1: Weigh and clean the Chinese medicine with antibacterial and anti-inflammatory effects in advance, then soak it in water for 30 minutes, bring the water to a boil, and keep it warm for 1 hour; Step 2: Cool the liquid to 80 degrees, filter it, and collect the filtrate; Step 3: Add bone glue to the 80-degree solution and keep stirring for 1 hour; Step 4: Cool the liquid to 40 degrees and add vitamin C and vitamin E; Step 5: Immerse the tanned collagen surgical suture thread in the drug solution, and load the drug solution onto the collagen surgical suture thread through multiple roller rolling; Step 6. Take out the collagen surgical sutures from the liquid medicine, dry them with cold air, and then sterilize them with high temperature. After completion, inspect them. If they pass the inspection, they will be packaged in a quantitative manner.

[0022] The Chinese medicines with antibacterial and anti-inflammatory effects in step one include forsythia, isatis root, honeysuckle, Panax notoginseng, and isatis indigotica. The weight and mass proportions of each raw material are as follows: forsythia 10, isatis root 8, honeysuckle 10, Panax notoginseng 6, isatis indigotica 5, and aqueous solution 100.

[0023] The bone glue in step 3 is set to be any one of bovine bone glue, pig bone glue, and sheep bone glue, and the mass percentage of bone glue is: 10.

[0024] The mass ratio of vitamin C and vitamin E to the gelatin-containing solution in step 4 is 2:1:100.

[0025] The collagen surgical suture thread in step five is immersed in the medicinal solution for 2 minutes, and the mass ratio of the collagen surgical suture thread is 50.

[0026] When drying the sutures in step six, do not bake them or expose them to direct sunlight. Instead, dry them naturally in a cool, dry place.

[0027] The collagen surgical suture thread that has passed the inspection in step 6 has a tensile strength of more than 16.9N, an increase of 25%, a flexibility of more than 10%, an antibacterial rate of 99.9%, a collagen purity of 95%, and a cytotoxicity of less than level 1. Example

[0028] The present invention provides a preparation technology of collagen surgical suture with antibacterial and anti-inflammatory functions, comprising the following steps: Step 1: Weigh and clean the Chinese medicine with antibacterial and anti-inflammatory effects in advance, then soak it in water for 30 minutes, bring the water to a boil, and keep it warm for 1 hour; Step 2: Cool the liquid to 80 degrees, filter it, and collect the filtrate; Step 3: Add bone glue to the 80-degree solution and keep stirring for 1 hour; Step 4: Cool the liquid to 40 degrees and add vitamin C and vitamin E; Step 5: Immerse the tanned collagen surgical suture thread in the drug solution, and load the drug solution onto the collagen surgical suture thread through multiple roller rolling; Step 6. Take out the collagen surgical sutures from the liquid medicine, dry them with cold air, and then sterilize them with high temperature. After completion, inspect them. If they pass the inspection, they will be packaged in a quantitative manner.

[0029] The Chinese medicine with antibacterial and anti-inflammatory effects in step one includes forsythia, isatis root, honeysuckle, Panax notoginseng, and isatis indigotica. The weight and mass proportions of each raw material are as follows: forsythia 8, isatis root 6, honeysuckle 8, Panax notoginseng 5, isatis indigotica 4, and aqueous solution 100.

[0030] The bone glue in step 3 is set to be any one of bovine bone glue, pig bone glue, and sheep bone glue, and the mass proportion of bone glue is: 7.5.

[0031] The mass ratio of vitamin C and vitamin E to the gelatin-containing solution in step 4 is 1.2:0.6:100.

[0032] The collagen surgical suture thread in step five is immersed in the medicinal solution for 1.2 minutes, and the mass ratio of the collagen surgical suture thread is 30.

[0033] When drying the sutures in step six, do not bake them or expose them to direct sunlight. Instead, dry them naturally in a cool, dry place.

[0034] The collagen surgical suture thread that has passed the inspection in step 6 has a tensile strength of more than 16.9N, an increase of 25%, a flexibility of more than 10%, an antibacterial rate of 99.9%, a collagen purity of 95%, and a cytotoxicity of less than level 1.

[0035] Control Example The present invention provides a preparation technology of collagen surgical suture with antibacterial and anti-inflammatory functions, comprising the following steps: Step 1: Weigh and clean the Chinese medicine with antibacterial and anti-inflammatory effects in advance, then soak it in water for 30 minutes, bring the water to a boil, and keep it warm for 1 hour; Step 2: Cool the liquid to 80 degrees, filter it, and collect the filtrate; Step 3: Add bone glue to the 80-degree solution and keep stirring for 1 hour; Step 4: Cool the liquid to 40 degrees and add vitamin C and vitamin E; Step 5: Immerse the tanned collagen surgical suture thread in the drug solution, and load the drug solution onto the collagen surgical suture thread through multiple roller rolling; Step 6. Take out the collagen surgical sutures from the liquid medicine, dry them with cold air, and then sterilize them with high temperature. After completion, inspect them. If they pass the inspection, they will be packaged in a quantitative manner.

[0036] The Chinese medicine with antibacterial and anti-inflammatory effects in step one includes forsythia, isatis root, honeysuckle, Panax notoginseng, and isatis indigotica. The weight and mass proportions of each raw material are as follows: forsythia 3, isatis root 12, honeysuckle 14, Panax notoginseng 2, isatis indigotica 8, and aqueous solution 100.

[0037] The bone glue in step 3 is set to be any one of bovine bone glue, pig bone glue, and sheep bone glue, and the mass proportion of bone glue is: 8.

[0038] The mass ratio of vitamin C and vitamin E to the gelatin-containing solution in step 4 is 0.2:3:100.

[0039] The collagen surgical suture thread in step five is immersed in the medicinal solution for 4 minutes, and the mass ratio of the collagen surgical suture thread is 20.

[0040] When drying the sutures in step six, do not bake them or expose them to direct sunlight. Instead, dry them naturally in a cool, dry place.

[0041] The collagen surgical suture thread that has passed the inspection in step 6 has a tensile strength of more than 16.9N, an increase of 25%, a flexibility of more than 10%, an antibacterial rate of 99.9%, a collagen purity of 95%, and a cytotoxicity of less than level 1.

[0042] Comprehensive comparison of multiple experiments shows that the effects of Example 1, Example 2 and Example 3 are better than that of the control example, and among Example 1, Example 2 and Example 3, Example 3 has better effects than Example 1 and Example 2.

[0043] The company has developed a pioneering "nutritional Chinese medicine coating" technology for goat intestine sutures. Using chitosan, vitamins, and Panax notoginseng as raw materials, a nutrient solution is heated and applied to the sutures, adding a "nutritional Chinese medicine coating" to the sutures. This coating enhances knotting safety and ensures simultaneous absorption by human tissue, providing antibacterial, antimicrobial, and anti-inflammatory benefits, reducing infection rates. After suturing 50 rabbits with leg and abdominal injuries using this "nutritional Chinese medicine coating," the sutures remained intact, showing no detachment or breakage. None of the rabbits' wounds developed infection, and the suture degradation synchronized with wound healing.

[0044] The above are only preferred specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with this technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solutions and inventive concepts of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A preparation technology for collagen surgical suture with antibacterial and anti-inflammatory functions, characterized by: The following steps are involved: Step 1: Weigh and clean the Chinese medicine with antibacterial and anti-inflammatory effects in advance, then soak it in water for 30 minutes, bring the water to a boil, and keep it warm for 1 hour; Step 2: Cool the liquid to 80 degrees, filter it, and collect the filtrate; Step 3: Add bone glue to the 80-degree solution and keep stirring for 1 hour; Step 4: Cool the liquid to 40 degrees and add vitamin C and vitamin E; Step 5: Immerse the tanned collagen surgical suture thread in the drug solution, and load the drug solution onto the collagen surgical suture thread through multiple roller rolling; Step 6. Take out the collagen surgical sutures from the liquid medicine, dry them with cold air, and then sterilize them with high temperature. After completion, inspect them. If they pass the inspection, they will be packaged in a quantitative manner.

2. The preparation technology of a collagen surgical suture with antibacterial and anti-inflammatory functions according to claim 1, characterized in that: The Chinese medicine with antibacterial and anti-inflammatory effects in step 1 includes forsythia, isatis root, honeysuckle, Panax notoginseng, and isatis indigotica. The weight and mass proportions of each raw material are as follows: forsythia 6-10, isatis root 4-8, honeysuckle 6-10, Panax notoginseng 4-6, isatis indigotica 3-5, and aqueous solution 100.

3. The preparation technology of the collagen surgical suture with antibacterial and anti-inflammatory functions according to claim 1 is characterized in that: The bone glue described in step 3 is set to be any one of bovine bone glue, pig bone glue, and sheep bone glue, and the mass proportion of the bone glue is 5 to 10.

4. The preparation technology of the collagen surgical suture with antibacterial and anti-inflammatory functions according to claim 1 is characterized in that: The mass ratio of vitamin C and vitamin E to the gelatin-containing liquid in step 4 is 0.5-2:0.1-1:

100.

5. The preparation technology of the collagen surgical suture with antibacterial and anti-inflammatory functions according to claim 1 is characterized in that: The collagen surgical suture thread in the step 5 is immersed in the liquid medicine for 0.5 to 2 minutes, and the mass ratio of the collagen surgical suture thread is 10 to 50.

6. The preparation technology of the collagen surgical suture with antibacterial and anti-inflammatory functions according to claim 1 is characterized in that: When drying the suture thread in step 6, it should not be baked or exposed to direct sunlight, but should be dried naturally in a cool and dry place.

7. The preparation technology of the collagen surgical suture with antibacterial and anti-inflammatory functions according to claim 1 is characterized in that: The collagen surgical suture thread that has passed the inspection in step 6 has a tensile strength of more than 16.9N, an increase of 25%, a flexibility of more than 10%, an antibacterial rate of 99.9%, a collagen purity of 95%, and a cytotoxicity of less than level 1.