Synchronous detection device for multiple food additives based on microfluidic technology

Abstract

The invention discloses a micro-fluidic technology-based multi-food additive synchronous detection device, which comprises a main body assembly, a liquid inlet mixing transmission part, a liquid outlet mixing transmission part, a liquid outlet mixing transmission part and a liquid outlet mixing transmission part, the micro-fluidic assembly comprises a plurality of side liquid inlet chips connected to the liquid inlet mixing transmission part in a sliding mode, side liquid inlet flow channels are formed in the side liquid inlet chips, a middle liquid inlet chip is connected to the center of the liquid inlet mixing transmission part in an inserted mode, and a liquid inlet counter bore is formed in the middle liquid inlet chip; a plurality of middle liquid inlet flow channels corresponding to the side liquid inlet flow channels in a one-to-one mode are arranged on the middle liquid inlet chip on the outer edge of the bottom of the liquid inlet counter bore, a plurality of reaction chips corresponding to the side liquid inlet chips in a one-to-one mode are arranged outside the liquid inlet mixing transmission piece, and inner liquid inlet flow channels capable of being communicated with the side liquid inlet flow channels are arranged on the reaction chips. Liquid in the liquid storage tank at one end, close to the periphery of the liquid inlet mixing transmission part, of the reaction chip can be mixed with liquid in the inner liquid inlet flow channel to enter the reaction tank; according to the invention, simultaneous detection of various food additives can be realized, and the detection efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of food additive detection, in particular to a multi-food additive synchronous detection device based on microfluidic technology. Background Art

[0002] With the development of the food industry, food additives are widely used, but problems such as abuse and excessive use threaten food safety. Currently, although there are a variety of detection technologies that can analyze common additives and illegal additives, there are limitations in detecting multiple additives simultaneously. Different additives have different chemical properties, and existing devices are mostly targeted at specific types, making it difficult to detect multiple types simultaneously. Repeated replacement of equipment and methods is required, resulting in cumbersome operations, high costs, and low efficiency. Developing devices that can detect multiple food additives simultaneously has become an urgent need in the industry to improve detection efficiency. Summary of the Invention

[0003] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of this application to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions should not be used to limit the scope of the present invention.

[0004] In view of the above and / or existing problems in food additive detection, the present invention is proposed.

[0005] Therefore, the purpose of the present invention is to provide a multi-food additive synchronous detection device based on microfluidic technology, which transmits the sample liquid to be tested into the reaction chip through the middle liquid inlet chip through multiple side liquid inlet chips. The detection liquid in several side liquid inlet chips also enters the reaction chip and mixes with the sample liquid to be tested. The color of the mixed solution at the reaction pool can be used to detect whether there are corresponding food additives in the sample liquid to be tested.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a multi-food additive synchronous detection device based on microfluidic technology, comprising: The main body assembly includes a fixed base with an upward opening, the fixed base is connected to a liquid inlet mixing transmission member, and a plurality of slide grooves are arranged on an upward end of the liquid inlet mixing transmission member; The microfluidic component includes several side liquid inlet chips which are respectively connected to the liquid inlet mixing transmission member through respective slide grooves. The side liquid inlet chip is provided with a side liquid inlet flow channel, and the liquid inlet mixing transmission member is provided with a plug-in sink groove in the shape of a regular polygon. The liquid inlet mixing transmission member is just plugged with an intermediate liquid inlet chip through the plug-in sink groove. The center of the intermediate liquid inlet chip is provided with a liquid inlet sink hole at the upward end. The periphery of the intermediate liquid inlet chip is provided with several intermediate liquid inlet flow channels which correspond one to one to the side liquid inlet flow channels. The outside of the liquid inlet mixing transmission member is provided with several reaction chips which correspond one to one to the side liquid inlet chips. The reaction chip is provided with an inner liquid inlet flow channel which can be connected with the side liquid inlet flow channel. The reaction chip is also provided with several liquid storage tanks at one end close to the outer periphery of the liquid inlet mixing transmission member. The reaction chip is provided with a reaction tank at one end away from the outer periphery of the liquid inlet mixing transmission member. The liquid in the liquid storage tank can be mixed with the liquid in the inner liquid inlet flow channel and enter the reaction tank.

[0007] The inner wall of the upper part of the middle liquid inlet chip has an internal thread, and the upper part of the middle liquid inlet chip is threadedly connected with a closing cover; before testing, the closing cover is unscrewed, and the sample liquid to be tested is injected into the middle liquid inlet chip. After the injection is completed, the closing cover is screwed in to make the middle liquid inlet chip airtight; the sample liquid to be tested in the middle liquid inlet chip flows into the side liquid inlet flow channel of the corresponding side liquid inlet chip through the middle liquid inlet flow channel, so that the sample liquid to be tested in the side liquid inlet flow channel flows toward the direction of the corresponding reaction chip, and the test liquid stored in the reaction chip flows toward the direction of the reaction pool, so that the sample liquid to be tested and the test liquid are mixed. If the sample liquid to be tested contains corresponding additives, the mixed liquid in the corresponding reaction pool has a corresponding color, thereby realizing simultaneous detection of multiple food additives.

[0008] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, the center of the fixed base is rotatably connected to a vertically arranged transmission shaft, and the liquid inlet mixing transmission component is fixedly connected to the transmission shaft.

[0009] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, wherein: the side liquid inlet chip is rotatably connected to a liquid outlet docking sleeve at one end outside the liquid inlet mixing transmission part, and a plurality of clamping blocks are arranged on the inner wall of the liquid outlet docking sleeve. The reaction chip includes a reaction chip body, and a support shaft is fixed on the side of the reaction chip body opposite to the side liquid inlet chip. The inner liquid inlet channel is arranged at the center of the support shaft, and a plurality of clamping grooves corresponding to the clamping parts are arranged on the periphery of the support shaft. The support shaft is clamped to the corresponding clamping block through the clamping groove.

[0010] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, the reaction chip also includes a transmission bevel gear, a circular socket is opened on the transmission bevel gear, and a connecting part corresponding to the two clamping grooves is fixed on the transmission bevel gear outside the socket. The transmission bevel gear is just inserted into the two clamping grooves through the connecting part and abuts against the shoulder of the support shaft. A support bracket is sleeved on the support shaft on the side of the transmission bevel gear away from the reaction chip body, and the end of the support bracket away from the transmission bevel gear is connected to the liquid inlet mixing transmission component, and the support shaft is rotatably connected to the support bracket.

[0011] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, wherein: the support bracket is connected to the support shaft on the side away from the transmission bevel gear, and the liquid storage chip body is provided with a circular mounting hole, and the inner wall of the liquid storage chip body on both sides of the mounting hole is fixed with a clamping part corresponding to two of the clamping grooves. After the clamping part of the liquid storage chip body is clamped on the support shaft through the corresponding two clamping grooves, the liquid storage chip body is fixedly connected to the support shaft.

[0012] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, wherein: the liquid storage chip body on both sides of the support shaft is respectively provided with a first liquid storage tank and a second liquid storage tank, the liquid storage chip body at the first liquid storage tank and the second liquid storage tank is threadedly connected with an inner sealing cover, a first liquid outlet channel is opened on the liquid storage chip body at one end of the first liquid storage tank relative to the support shaft, a second liquid outlet channel is opened on the liquid storage chip body at one end of the second liquid storage tank relative to the support shaft, a first liquid outlet channel and a second liquid outlet channel are opened on the support shaft, one end of the first intermediate channel is connected to the end of the first liquid outlet channel, one end of the second intermediate channel is connected to the end of the second liquid outlet channel, and the support shaft is provided with A first intermediate liquid outlet channel and a second intermediate liquid outlet channel are respectively provided on the reaction chip body at both ends of the support shaft. The other end of the first intermediate channel is connected to the head end of the first intermediate liquid outlet channel, and the other end of the second intermediate channel is connected to the head end of the second intermediate liquid outlet channel. The end of the first intermediate liquid outlet channel is connected to the end of the second intermediate liquid outlet channel. A drainage channel is provided on the reaction chip body at the junction of the ends of the first intermediate liquid outlet channel and the second intermediate liquid outlet channel. A connecting channel is provided on the reaction chip body at the end of the inner liquid inlet channel. The side of the connecting channel away from the end of the inner liquid inlet channel is connected to the drainage channel, and one end of the drainage channel away from the first intermediate liquid outlet channel is connected to the reaction pool.

[0013] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, wherein: a transmission ring is rotatably connected to the fixed base, and a plurality of transmission teeth meshing with the transmission bevel gear are arranged on the upper end of the transmission ring.

[0014] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, wherein: the inner end of the side liquid inlet chip is fixed with a limiting part, the periphery of the liquid inlet mixing transmission component is arranged with through holes corresponding one to one with the limiting part, and the periphery of the middle liquid inlet chip is arranged with a number of limiting grooves corresponding one to one with the limiting part. When the bottom side of the middle liquid inlet chip contacts the liquid inlet mixing transmission component on the lower side of the plug-in groove, the limiting part can pass through the corresponding through hole and be just plugged into the corresponding limiting groove.

[0015] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, wherein: the lower part of the fixed base is fixedly connected to a transmission motor, and the transmission shaft is connected to the transmission motor.

[0016] As a preferred solution of the multi-food additive synchronous detection device based on microfluidic technology in the present invention, at least two driving motors are arranged in the fixed base, a driving gear is connected to the driving motor, and a plurality of driven teeth meshing with the driving gear are arranged on the inner side of the transmission ring. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. Among them: Figure 1 It is a three-dimensional structural diagram of the present invention.

[0018] Figure 2 for Figure 1 A partial enlarged view of point A in the middle.

[0019] Figure 3 It is a three-dimensional structural diagram of the side liquid inlet chip in the present invention.

[0020] Figure 4 It is a three-dimensional structural diagram of the reaction chip in the present invention.

[0021] Figure 5 This is a three-dimensional structural diagram of the reaction chip in the present invention when it is set to a transparent state.

[0022] Figure 6 It is a three-dimensional structural diagram of the middle liquid inlet chip in the present invention.

[0023] Figure 7 It is a three-dimensional structural diagram of the liquid-intake mixing transmission component in the present invention.

[0024] Figure 8 This is a three-dimensional structural diagram of the entire structure of the fixed base and the lower shell in the present invention when they are set to a transparent state.

[0025] Figure 9 for Figure 8 A partial enlarged view of point B in the middle.

[0026] In the figure, 100 microfluidic component, 101 liquid storage chip body, 1011 inner sealing cover, 1012 fixing bolt, 1013 transmission bevel gear, 1014 support bracket, 1014-1 push-pull plate, 1015 liquid storage chip body, 1015-1 connecting through hole, 1015-2 clamping part, 1015-3 liquid storage tank, 1015-4 first liquid outlet channel, 1015-5 second liquid outlet channel, 1016 support shaft, 1016-1 inner liquid inlet channel, 1016-2 clamping sink, 1017 reaction chip body, 1017-1 reaction tank, 1017-2 second middle liquid outlet channel, 1017-3 first middle liquid outlet channel, 1017-4 drainage channel, 1017-5 connecting channel, 1017-6 outer liquid inlet channel Channel, 1018 outer sealing cover, 102 side liquid inlet chip, 1021 side liquid inlet flow channel, 1022 sliding part, 1023 limiting part, 103 intermediate liquid inlet chip, 1031 intermediate liquid inlet flow channel, 1032 limiting sink groove, 1033 intermediate liquid inlet sink hole, 104 liquid outlet docking sleeve, 1041 clamping block, 105 closing cover, 200 main body assembly, 201 liquid inlet mixing transmission part, 2011 slide groove, 2012 limiting sliding sink groove, 2013 plug sink groove, 2014 limiting hole, 202 fixed base, 203 support frame, 204 lower shell, 300 transmission ring, 301 transmission tooth, 302 driven tooth, 303 rotating part, 400 transmission shaft, 500 transmission motor, 600 driving gear, 700 drive motor, 800 limiting plate. DETAILED DESCRIPTION

[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0028] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0029] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0030] Example 1: Reference Figures 1 to 8, this embodiment provides a multi-food additive synchronous detection device based on microfluidic technology, which can realize the detection of different food additives.

[0031] A multi-food additive synchronous detection device based on microfluidic technology includes a main body component 200, which includes a fixed base 202 with an upward opening, a liquid inlet mixing transmission component 201 is connected above the fixed base 202, and a plurality of slide grooves 2011 are arranged on the upward end of the liquid inlet mixing transmission component 201, and a plurality of mounting ports connected to the slide grooves 2011 are arranged on the liquid inlet mixing transmission component 201 outside the slide grooves 2011, and limited sliding grooves 2012 are provided on the liquid inlet mixing transmission component 201 on both sides of the outer edge of the slide groove 2011, and a detachable microfluidic component 100 is connected to the liquid inlet mixing transmission component 201.

[0032] Specifically, the microfluidic component 100 includes a plurality of side liquid inlet chips 102 that are respectively connected to the liquid inlet mixing transmission member 201 through respective chute 2011. A sliding portion 1022 corresponding to the limit chute 2011 is fixed on the side liquid inlet chip 102. The side liquid inlet chip 102 slides along the chute 2011, and the sliding portion 1022 slides along the limit sliding trough 2012. When the side liquid inlet chip 102 moves inward to a position where it can no longer move, the side liquid inlet chip 102 is The liquid inlet chip 102 is installed in place, and the limit sliding sink groove 2012 limits the movement of the side liquid inlet chip 102 in the height direction. The side liquid inlet channel 1021 is opened on the side liquid inlet chip 102, and the liquid inlet mixing transmission element 201 is opened with a regular polygonal plug-in sink groove 2013. The liquid inlet mixing transmission element 201 is just plugged with the middle liquid inlet chip 103 through the plug-in sink groove 2013. The center of the middle liquid inlet chip 103 is opened with a liquid inlet sink hole at the upper end. The middle liquid inlet chip 103 is opened with a liquid inlet sink hole. The outer periphery of 03 is arranged with a plurality of intermediate liquid inlet channels 1031 corresponding to the side liquid inlet channels 1021. The center of the upper end of the intermediate liquid inlet chip 103 is provided with an intermediate liquid inlet sink hole 1033. The intermediate liquid inlet sink hole 1033 is connected to the plurality of intermediate liquid inlet channels 1031. The liquid inlet mixing transmission element 201 is arranged with a plurality of reaction chips corresponding to the side liquid inlet chips 102. The reaction chip has an inner liquid inlet channel 1031 that can be connected to the side liquid inlet channel 1021. 016-1, a plurality of liquid storage tanks 1015-3 are provided at one end of the reaction chip close to the outer periphery of the liquid inlet mixing transmission component 201, and a reaction tank 1017-1 is provided at one end of the reaction chip away from the outer periphery of the liquid inlet mixing transmission component 201. An outer sealing cover 1018 is threadedly connected to the reaction chip body 1017 at the reaction tank 1017-1, and the liquid in the liquid storage tank 1015-3 can be mixed with the liquid in the internal liquid inlet channel 1016-1 and enter the reaction tank 1017-1.

[0033] The inner wall of the upper part of the middle liquid inlet chip 103 has an internal thread, and the upper part of the middle liquid inlet chip 103 is threadedly connected to a closing cover 105, which can fit on the step on the upper side of the middle liquid inlet countersunk hole 1033; before testing, the closing cover 105 is unscrewed, and the sample liquid to be tested is injected into the middle liquid inlet chip 103. After the injection is completed, the closing cover 105 is screwed in to seal the middle liquid inlet chip 103; the sample liquid to be tested in the middle liquid inlet chip 103 flows into the side liquid inlet channel 1021 of the corresponding side liquid inlet chip 102 through the middle liquid inlet channel 1031, so that the sample liquid to be tested in the side liquid inlet channel 1021 flows toward the direction of the corresponding reaction chip, and the test liquid stored in the reaction chip flows toward the direction of the reaction pool 1017-1, so that the sample liquid to be tested and the test liquid are mixed. If the sample liquid to be tested contains corresponding additives, the mixed liquid in the corresponding reaction pool 1017-1 has a corresponding color, thereby realizing simultaneous detection of multiple food additives.

[0034] Example 2: Reference Figures 1 to 9 This embodiment provides a multi-food additive synchronous detection device based on microfluidic technology, which can further realize the inflow of the sample liquid to be detected and the detection liquid, as well as the sufficient mixing of various solutions.

[0035] Specifically, a lower shell 204 with an upward opening is fixedly connected to the upper side of the fixed base 202, a support frame 203 is fixedly connected inside the lower shell 204, and a vertically arranged transmission shaft 400 is rotatably connected to the center of the support frame 203. The liquid inlet mixing transmission member 201 is fixedly connected to the transmission shaft 400, and the side liquid inlet chip 102 is rotatably connected to the liquid outlet docking sleeve 104 at one end outside the liquid inlet mixing transmission member 201. A plurality of clamping blocks 1041 are arranged on the inner wall of the liquid outlet docking sleeve 104. The reaction chip includes a reaction chip body 1017, and a support shaft 1016 is fixed to the side of the reaction chip body 1017 opposite to the side liquid inlet chip 102. The inner liquid inlet channel 1016-1 is arranged at the center of the support shaft 1016. The reaction chip body 1017 is provided with an outer liquid inlet channel 1017-6 that is connected to the inner liquid inlet channel 1016-1. The head end of the outer liquid inlet channel 1017-6 is connected to the inner liquid inlet channel The end of the channel 1016-1 is connected, the end of the external liquid inlet channel 1017-6 is connected to the reaction tank 1017-1, and a plurality of clamping grooves 1016-2 are arranged on the periphery of the support shaft 1016. The support shaft 1016 is clamped on the corresponding clamping block 1041 through the clamping groove 1016-2. A limiting block is fixed on the side of the side liquid inlet chip 102 away from the support shaft 1016. A plurality of limiting blocks are arranged on the liquid inlet mixing transmission member 201. Corresponding to the limiting holes 2014, the periphery of the middle liquid inlet chip 103 is arranged with a number of limiting grooves 1032 corresponding to the limiting blocks; when the side liquid inlet chip 102 is installed on the liquid inlet mixing transmission component 201, the limiting blocks of the liquid inlet mixing transmission component 201 pass through the corresponding limiting holes 2014 and are inserted into the limiting grooves 1032, thereby realizing the limitation of the middle liquid inlet chip 103 in the height direction and improving the reliability of the rotational liquid inlet of the middle liquid inlet chip 103.

[0036] The central axis of the liquid inlet mixing transmission component 201 is perpendicular to the central axis of the support shaft 1016. When liquid is introduced, the transmission shaft 400 rotates, and the transmission shaft 400 drives the liquid inlet mixing transmission component 201 to rotate. The transmission shaft 400 drives the support shaft 1016 to rotate through the liquid outlet docking sleeve 104, and the support shaft 1016 drives the reaction chip body 1017 to rotate. The sample liquid to be tested entering the middle liquid inlet chip 103 passes through the side liquid inlet channel 1021 and is quickly thrown into the inner liquid inlet channel 1016-1 of the support shaft 1016. The sample liquid to be tested entering the inner liquid inlet channel 1016-1 enters the reaction chip body 1017.

[0037] Specifically, the reaction chip also includes a transmission bevel gear 1013, a transmission ring 300 is rotatably connected to the lower shell 204, an intermediate connecting portion is fixed to the lower end of the transmission ring 300, a rotating portion 303 is fixed to the lower side of the intermediate connecting portion, a rotating groove is opened at the upper end of the lower shell 204, the rotating portion 303 of the transmission ring 300 is rotatably connected in the rotating groove, a plurality of limiting holes 2014 are arranged on the lower shell 204 at the outer edge of the rotating groove, a limiting plate 800 is inserted into the limiting hole 2014 of the lower shell 204, and the lower side of the limiting plate 800 is attached to the upper side of the rotating portion 303 to limit the movement of the rotating portion 303 in the height direction, and the upper end of the transmission ring 300 is arranged There are a number of transmission teeth 301 that mesh with the transmission bevel gear 1013. A circular socket is opened on the transmission bevel gear 1013. Two connecting parts corresponding to the card-engaging grooves 1016-2 are fixed on the transmission bevel gear 1013 outside the socket. The transmission bevel gear 1013 is just plugged into the two card-engaging grooves 1016-2 through the connecting parts. The outer side of the transmission bevel gear 1013 in the axial direction is in contact with the shoulder of the support shaft 1016. The support shaft 1016 on the side of the transmission bevel gear 1013 away from the reaction chip body 1017 is sleeved with a support bracket 1014. The end of the support bracket 1014 away from the transmission bevel gear 1013 is connected to the inlet On the liquid mixing transmission component 201 (a plurality of fixing ears are arranged on the upper end of the liquid mixing transmission component 201, and the support bracket 1014 is fixedly connected to the fixing ears using fixing bolts 1012), the support shaft 1016 is rotatably connected to the support bracket 1014, and the support shaft 1016 on the side of the support bracket 1014 away from the transmission bevel gear 1013 is connected to the liquid storage chip body 1015101, and a circular mounting hole is opened on the liquid storage chip body 1015101. The inner wall of the liquid storage chip body 1015101 on both sides of the mounting hole is fixed with a clamping portion 1015-2 corresponding to two of the clamping grooves 1016-2. The liquid storage chip body 1015101 is provided with a plurality of fixing ears. After the clamping portion 1015-2 of 15101 is clamped onto the support shaft 1016 through the corresponding two clamping grooves 1016-2, the liquid storage chip body 1015101 is fixedly connected to the support shaft 1016. The fixed connection structure is that at least two connecting through holes 1015-1 are opened on the liquid storage chip body 1015101 at the positions of the two clamping portions 1015-2, and the support shaft 1016 is also provided with connecting countersunk holes corresponding to the connecting through holes 1015-1. The fastening bolts are respectively screwed into the connecting through holes 1015-1 and then screwed into the connecting countersunk holes, so that the liquid storage chip body 1015101 is fixedly connected to the support shaft 1016.

[0038] When the reaction chip as a whole rotates around the central axis of the transmission shaft 400, the support shaft 1016 rotates in the support bracket 1014. At the same time, the support bracket 1014 plays a role in limiting the axial movement of the reaction chip, thereby improving the reliability of the revolution of the reaction chip. In addition, the inner end of the support shaft 1016 is inserted into the liquid outlet docking sleeve 104 of the side liquid inlet chip 102, which can also limit the axial movement of the side liquid inlet chip 102. In addition, when a new microfluidic component 100 needs to be replaced, the fixing bolt 1012 is unscrewed to loosen the support bracket 1014 and the liquid inlet mixing transmission component 201, and the reaction chip is pulled out through the push-pull plate 1014-1 at the upper end of the support bracket 1014, and the side liquid inlet chip 102 is moved outward to make the limit block leave the limit hole 2014, and the old side liquid inlet chip 102 and the old middle liquid inlet chip 103 are taken out, and the old reaction chip and the old side liquid inlet chip 102 can be replaced. The chip 102 and the old middle liquid inlet chip 103 are separated; the new middle liquid inlet chip 103 is aligned with the plug-in groove 2013, so that the middle liquid inlet chip 103 is just plugged into the center of the liquid inlet mixing transmission component 201, and the bottom side of the middle liquid inlet chip 103 is in contact with the liquid inlet mixing transmission component 201, and the side liquid inlet chip 102 is inserted into the liquid inlet mixing transmission component 201, and the inner end of the side liquid inlet chip 102 is pressed tightly against the outside of the middle liquid inlet chip 103, and the snap-in groove 1016-2 on the support shaft 1016 is aligned with the snap-in block 1041 on the inner side of the liquid outlet docking sleeve 104, and the push-pull plate 1014-1 is inserted between the corresponding two fixed ears, and the fixing bolts 1012 are screwed into the fixed ears and the push-pull plate 1014-1 to fix the support bracket 1014 on the liquid inlet mixing transmission component 201, so as to realize the axial positioning of the entire reaction chip, and the microfluidic component 100 is easy to disassemble and maintain.

[0039] Specifically, the liquid storage chip body 1015101 on both sides of the support shaft 1016 has a first liquid storage tank 1015-3 and a second liquid storage tank 1015-3 respectively. The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 are both threadedly connected to the liquid storage chip body 1015101 with an inner sealing cover 1011. The liquid storage chip body 1015101 at one end of the first liquid storage tank 1015-3 relative to the support shaft 1016 has a first liquid outlet channel 1015-4 and a second liquid outlet channel 1015-5. The liquid storage tank 1015-3 is provided with a second liquid outlet channel 1015-5 on the liquid storage chip body 1015101 at one end of the support shaft 1016. The support shaft 1016 is provided with a first liquid outlet channel 1015-4 and a second liquid outlet channel 1015-5. One end of the first intermediate channel is connected to the end of the first liquid outlet channel 1015-4, and one end of the second intermediate channel is connected to the end of the second liquid outlet channel 1015-5. The reaction chip body 1015101 at both ends of the support shaft 1016 is provided with a first liquid outlet channel 1015-4 and a second liquid outlet channel 1015-5. 17 is respectively provided with a first intermediate liquid outlet channel 1017-3 and a second intermediate liquid outlet channel 1017-2, the other end of the first intermediate liquid outlet channel is connected to the head end of the first intermediate liquid outlet channel 1017-3, the other end of the second intermediate liquid outlet channel is connected to the head end of the second intermediate liquid outlet channel 1017-2, the end of the first intermediate liquid outlet channel 1017-3 is connected to the end of the second intermediate liquid outlet channel 1017-2, and the first intermediate liquid outlet channel 1017-3 and the second intermediate liquid outlet channel are connected. A drainage channel 1017-4 is provided on the reaction chip body 1017 at the junction of the ends of the inner and outer liquid inlet channels 1017-2, and a connecting channel 1017-5 is provided on the reaction chip body 1017 at the end of the outer liquid inlet channel 1017-6. The side of the connecting channel 1017-5 away from the end of the inner liquid inlet channel 1016-1 is connected to the drainage channel 1017-4, and the end of the drainage channel 1017-4 away from the first intermediate liquid outlet channel 1017-3 is connected to the reaction pool 1017-1.

[0040] When the reaction chip revolves, the liquid in the first liquid storage tank 1015-3 and the liquid in the second liquid storage tank 1015-3 flow into the first intermediate flow channel and the second intermediate flow channel through the first liquid outlet channel 1015-4 and the second liquid outlet channel 1015-5 respectively, and then are respectively thrown into the discharge flow channel 1017-4 through the first intermediate liquid outlet channel 1017-3 and the second intermediate liquid outlet channel 1017-2. The sample liquid to be tested is also thrown into the discharge flow channel 1017-4 after passing through the inner liquid inlet channel 1016-1, the outer liquid inlet channel 1017-6 and the connecting flow channel 1017-5 in sequence. The sample liquid to be tested and the various test liquids are mixed and reacted, causing the transmission bevel gear 1013 to rotate. The transmission bevel gear 1013 drives the reaction chip body 1017 to rotate through the support shaft 1016. The reaction chip body 1017 rotates, so that the sample liquid to be tested and the various test liquids are fully mixed, which is convenient for subsequent component detection of the sample liquid to be tested.

[0041] Specifically, the inner end of the side liquid inlet chip 102 is fixed with a limiting portion 1023, and the axial lengths of the sliding portion 1022 and the limiting portion 1023 are not greater than the distance between the outer side of the liquid outlet docking sleeve 104 and the outer edge of the mounting port (this description corresponds to the position of the side liquid inlet chip 102 when it is installed in place), ensuring that when the side liquid inlet chip 102 moves in the direction of the outer edge of the mounting port, the limiting portion 1023 and the sliding portion 1022 can both be separated from the liquid inlet mixing transmission member 201, so that the side liquid inlet chip 102 can be It is smoothly taken out from the liquid inlet mixing transmission component 201; the outer periphery of the liquid inlet mixing transmission component 201 is arranged with through holes corresponding one to one with the limiting parts 1023, and the outer periphery of the middle liquid inlet chip 103 is arranged with a number of limiting grooves 1032 corresponding one to one with the limiting parts 1023. When the bottom side of the middle liquid inlet chip 103 contacts the liquid inlet mixing transmission component 201 on the lower side of the plug-in groove 2013, the limiting part 1023 can pass through the corresponding through hole and be just plugged into the corresponding limiting groove 1032.

[0042] Specifically, a transmission motor 500 is fixedly connected to the fixed base 202 , and the transmission shaft 400 is connected to the transmission motor 500 .

[0043] After the closing cover 105 is screwed into the middle liquid inlet chip 103, the transmission motor 500 is controlled to operate, the transmission shaft 400 rotates, and the transmission shaft 400 drives the side liquid inlet chip 102 and the reaction chip to revolve through the liquid inlet mixing transmission element 201, further increasing the liquid inlet speed.

[0044] Specifically, at least two drive motors 700 are fixedly connected to the lower part of the support frame 203. In this embodiment, two drive motors 700 are fixedly connected to the support frame 203 of the lower shell 204. The drive motor 700 is connected to a driving gear 600. A plurality of driven teeth 302 are arranged on the inner side of the transmission ring 300, and the driving gear 600 and the driven teeth 302 are engaged.

[0045] After the liquid is added, the two drive motors 700 are controlled to operate simultaneously, the driving gear 600 rotates, the driving gear 600 drives the transmission ring 300 to rotate via the driven gear 302, the transmission ring 300 drives the transmission bevel gear 1013 to rotate via the transmission gear 301, and the transmission bevel gear 1013 drives the reaction chip to rotate, so that the sample liquid to be tested and various detection liquids are fully mixed and reacted, and the mixing speed of the liquids is accelerated.

[0046] In this embodiment, there are 8 reaction chips, and the 8 liquid storage chip bodies 1015101 are named as the first liquid storage chip body 1015101, the second liquid storage chip body 1015101, the third liquid storage chip body 1015101, the fourth liquid storage chip body 1015101, the fifth liquid storage chip body 1015101, the sixth liquid storage chip body 1015101, the seventh liquid storage chip body 1015101 and the eighth liquid storage chip body 1015101. The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 in the first liquid storage chip body 1015101 respectively store 0.05M iodine solution (dissolve 12.7g iodine and 40g potassium iodide in water, dilute to 1L, form iodine solution, and store in a brown bottle) and 1M sodium hydroxide solution (dissolve 40g NaOH in water to form sodium hydroxide solution, cool and dilute to 1L). When the sample liquid to be tested contains detectable saccharin sodium, it is expected that a characteristic change of producing a large amount of flocculent or flaky yellow precipitates will be observed in the corresponding reaction tank 1017-1. If no such characteristic precipitate is generated, it indicates that the sample liquid to be tested may not contain detectable saccharin sodium, and further testing will be carried out to confirm it.

[0047] The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 in the second liquid storage chip body 1015101 respectively store sulfanilic acid solution (dissolve 1g sulfanilic acid in 100ml 10% HCl to form a sulfanilic acid solution, stored in a brown bottle) and N-(1-naphthyl)ethylenediamine dihydrochloride solution (dissolve 0.1g N-(1-naphthyl)ethylenediamine dihydrochloride in 100ml water to form an N-(1-naphthyl)ethylenediamine dihydrochloride solution, stored in a brown bottle). When the sample liquid to be tested contains detectable sodium nitrite, it is expected that a characteristic color change from pink to red will be observed in the corresponding reaction tank 1017-1. If there is no such characteristic color change, it indicates that the sample liquid to be tested may not contain detectable sodium nitrite, and further testing will be performed to confirm it.

[0048] The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 in the third liquid storage chip body 1015101 respectively store a para-fuchsin hydrochloric acid solution (dissolve 0.2g of para-fuchsin in 120ml of concentrated hydrochloric acid, add water to make up to 200ml to form a para-fuchsin hydrochloric acid solution, stored in a brown bottle) and a formaldehyde solution (0.2% v / v, take 0.2ml of 37% formaldehyde and add 100ml of water to form a formaldehyde solution). When the sample liquid to be tested contains detectable sodium sulfite, it is expected that a characteristic color change of the solution to purple will be observed in the corresponding reaction tank 1017-1. If there is no such characteristic color change, it indicates that the sample liquid to be tested may not contain detectable sodium sulfite, and further testing will be carried out to confirm it.

[0049] The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 in the fourth liquid storage chip body 1015101 respectively store potassium bromate-potassium bromide solution (dissolve 1g potassium bromate and 2g potassium bromide in 100ml water to form a potassium bromate-potassium bromide solution) and 1M sulfuric acid solution (dissolve 5.5ml concentrated sulfuric acid in water and adjust the volume to 100ml to form a sulfuric acid solution). When the sample liquid to be tested contains detectable potassium sorbate, it is expected that a characteristic change in the yellow color of the solution will be observed in the corresponding reaction tank 1017-1 (becoming colorless or nearly colorless). If there is no such characteristic fading phenomenon, it indicates that the sample liquid to be tested may not contain detectable potassium sorbate, and further testing will be performed to confirm it.

[0050] The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 in the fifth liquid storage chip body 1015101 respectively store ammonium molybdate sulfuric acid solution (5g of ammonium molybdate is dissolved in 100ml of 0.5M H2SO4 to form an ammonium molybdate sulfuric acid solution) and stannous chloride solution (0.5g of stannous chloride is dissolved in 100ml of 0.5M HCl to form a stannous chloride solution, stored in a brown bottle). When the sample liquid to be tested contains detectable sodium dihydrogen phosphate, it is expected that a characteristic color change of dark blue (phosphomolybdenum blue) will be observed in the corresponding reaction pool 1017-1. If there is no such characteristic color change, it indicates that the sample liquid to be tested may not contain detectable sodium dihydrogen phosphate, and further testing will be performed to confirm it.

[0051] The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 in the sixth liquid storage chip body 1015101 respectively store 4-aminoantipyrine solution (0.5g of 4-aminoantipyrine is dissolved in 100ml of 0.1M sodium bicarbonate buffer to form a 4-aminoantipyrine solution, and the pH value of the 4-aminoantipyrine solution is 9.5) and potassium ferricyanide solution (0.8g of potassium ferricyanide is dissolved in 100ml of water to form a potassium ferricyanide solution). When the sample liquid to be tested contains detectable butylated hydroxyanisole (BHA), it is expected that a characteristic red color change of the solution will be observed in the corresponding reaction tank 1017-1. If there is no such characteristic color change, it indicates that the sample liquid to be tested may not contain detectable BHA, and further testing will be performed to confirm it.

[0052] The first liquid reservoir 1015-3 and the second liquid reservoir 1015-3 within the seventh liquid storage chip body 1015101 respectively store ninhydrin solution (dissolve 0.2g ninhydrin in 100ml ethanol to form a ninhydrin solution, stored in a brown bottle) and acetate buffer (dissolve 13.6g sodium acetate in water, add glacial acetic acid to adjust the pH to 5.0, and dilute to 1L to form acetate buffer). When the sample liquid to be tested contains detectable aspartame, it is expected that the solution in the corresponding reaction pool 1017-1 will show a characteristic blue-purple (or purple) color change. If this characteristic color change is not observed, it indicates that the sample liquid to be tested may not contain detectable aspartame, and further testing will be required to confirm it.

[0053] The first liquid storage tank 1015-3 and the second liquid storage tank 1015-3 in the eighth liquid storage chip body 1015101 respectively store a zinc powder suspension (suspend 1g of zinc powder in 100ml of water and shake to form a zinc powder suspension) and a 1M hydrochloric acid solution (dissolve 8.3ml of concentrated hydrochloric acid in water and dilute to 100ml to form a hydrochloric acid solution). When the sample liquid to be tested contains detectable tartrazine, it is expected that a characteristic change in the yellow color of the solution will be observed in the corresponding reaction tank 1017-1 (becoming colorless or light in color). If this characteristic fading phenomenon is not observed, it indicates that the sample liquid to be tested may not contain detectable tartrazine, and further testing will be required to confirm it.

[0054] Through the above implementation method, the simultaneous detection of 8 kinds of food additives can be achieved, and the detection is convenient and efficient.

[0055] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A device for simultaneous detection of multiple food additives based on microfluidic technology, characterized by: These include, The main body assembly includes a fixed base with an upward opening, the fixed base is connected to a liquid inlet mixing transmission member, and a plurality of slide grooves are arranged on an upward end of the liquid inlet mixing transmission member; The microfluidic component includes several side liquid inlet chips which are respectively connected to the liquid inlet mixing transmission member through respective slide grooves. The side liquid inlet chip is provided with a side liquid inlet flow channel, and the liquid inlet mixing transmission member is provided with a plug-in sink groove in the shape of a regular polygon. The liquid inlet mixing transmission member is just plugged with an intermediate liquid inlet chip through the plug-in sink groove. The center of the intermediate liquid inlet chip is provided with a liquid inlet sink hole at the upward end. The periphery of the intermediate liquid inlet chip is provided with several intermediate liquid inlet flow channels which correspond one to one to the side liquid inlet flow channels. The outside of the liquid inlet mixing transmission member is provided with several reaction chips which correspond one to one to the side liquid inlet chips. The reaction chip is provided with an inner liquid inlet flow channel which can be connected with the side liquid inlet flow channel. The reaction chip is also provided with several liquid storage tanks at one end close to the outer periphery of the liquid inlet mixing transmission member. The reaction chip is provided with a reaction tank at one end away from the outer periphery of the liquid inlet mixing transmission member. The liquid in the liquid storage tank can be mixed with the liquid in the inner liquid inlet flow channel and enter the reaction tank.

2. The device for simultaneous detection of multiple food additives based on microfluidic technology according to claim 1, characterized in that: The center of the fixed base is rotatably connected to a vertically arranged transmission shaft, and the liquid inlet mixing transmission component is fixedly connected to the transmission shaft.

3. The device for simultaneous detection of multiple food additives based on microfluidic technology according to claim 1, characterized in that: The side liquid inlet chip is rotatably connected to a liquid outlet docking sleeve at one end outside the liquid inlet mixing transmission component, and a plurality of clamping blocks are arranged on the inner wall of the liquid outlet docking sleeve. The reaction chip includes a reaction chip body, and a support shaft is fixed to the side of the reaction chip body opposite to the side liquid inlet chip. The inner liquid inlet channel is arranged at the center of the support shaft, and a plurality of clamping grooves corresponding to the clamping parts are arranged on the outer periphery of the support shaft. The support shaft is clamped to the corresponding clamping block through the clamping groove.

4. The device for simultaneous detection of multiple food additives based on microfluidic technology according to claim 3, characterized in that: The reaction chip also includes a transmission bevel gear, which has a circular socket. A connecting portion corresponding to two clamping grooves is fixed on the transmission bevel gear outside the socket. The transmission bevel gear is just inserted into the two clamping grooves through the connecting portion and abuts against the shoulder of the support shaft. A support bracket is sleeved on the support shaft on the side of the transmission bevel gear away from the reaction chip body. One end of the support bracket away from the transmission bevel gear is connected to the liquid inlet mixing transmission component, and the support shaft is rotatably connected to the support bracket.

5. The device for simultaneous detection of multiple food additives based on microfluidic technology according to claim 4, characterized in that: The support bracket is connected to the support shaft on the side away from the transmission bevel gear. The liquid storage chip body is provided with a circular mounting hole. The inner wall of the liquid storage chip body on both sides of the mounting hole is fixed with a clamping portion corresponding to two of the clamping grooves. After the clamping portion of the liquid storage chip body is clamped on the support shaft through the corresponding two clamping grooves, the liquid storage chip body is fixedly connected to the support shaft.

6. The device for simultaneous detection of multiple food additives based on microfluidic technology according to claim 5, characterized in that: The liquid storage chip bodies on both sides of the support shaft are respectively provided with a first liquid storage tank and a second liquid storage tank, and the liquid storage chip bodies at the first liquid storage tank and the second liquid storage tank are both threadedly connected with an inner sealing cover, a first liquid outlet channel is opened on the liquid storage chip body at one end of the first liquid storage tank relative to the support shaft, a second liquid outlet channel is opened on the liquid storage chip body at one end of the second liquid storage tank relative to the support shaft, a first liquid outlet channel and a second liquid outlet channel are opened on the support shaft, one end of the first intermediate channel is connected to the end of the first liquid outlet channel, one end of the second intermediate channel is connected to the end of the second liquid outlet channel, and the reaction chip bodies at both ends of the support shaft are respectively provided with a first intermediate channel. The intermediate liquid outlet channel and the second intermediate liquid outlet channel, the other end of the first intermediate liquid outlet channel is connected to the head end of the first intermediate liquid outlet channel, the other end of the second intermediate liquid outlet channel is connected to the head end of the second intermediate liquid outlet channel, the end of the first intermediate liquid outlet channel is connected to the end of the second intermediate liquid outlet channel, a drainage channel is opened on the reaction chip body at the junction of the ends of the first intermediate liquid outlet channel and the second intermediate liquid outlet channel, a connecting channel is opened on the reaction chip body at the end of the inner liquid inlet channel, the side of the connecting channel away from the end of the inner liquid inlet channel is connected to the drainage channel, and one end of the drainage channel away from the first intermediate liquid outlet channel is connected to the reaction pool.

7. The device for simultaneous detection of multiple food additives based on microfluidic technology according to any one of claims 4 to 6, characterized in that: A transmission ring is rotatably connected to the fixed base, and a plurality of transmission teeth meshing with the transmission bevel gear are arranged on the upper end of the transmission ring.

8. The device for simultaneous detection of multiple food additives based on microfluidic technology according to claim 7, characterized in that: A limiting portion is fixed at the inner end of the side liquid inlet chip, and through holes corresponding to the limiting portions are arranged on the periphery of the liquid inlet mixing transmission component. A number of limiting grooves corresponding to the limiting portions are arranged on the periphery of the middle liquid inlet chip. When the bottom side of the middle liquid inlet chip contacts the liquid inlet mixing transmission component on the lower side of the plug-in groove, the limiting portion can pass through the corresponding through hole and be plugged into the corresponding limiting groove.

9. The device for simultaneous detection of multiple food additives based on microfluidic technology according to any one of claims 2 to 6, characterized in that: The lower part of the fixed base is fixedly connected with a transmission motor, and the transmission shaft is connected to the transmission motor.

10. The device for simultaneous detection of multiple food additives based on microfluidic technology according to claim 7, characterized in that: At least two driving motors are arranged in the fixed base, and the driving motors are connected to driving gears. A plurality of driven teeth meshing with the driving gears are arranged inside the transmission ring.

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