Cell section sampling device

Abstract

The invention discloses a cell slice sampling device, which belongs to the technical field of biomedical detection equipment, and comprises a sampling table and a sample fixing mechanism arranged on the sampling table, the three-dimensional adjusting mechanism comprises an X / Y-axis translation assembly and a Z-axis feeding assembly, the X / Y-axis translation assembly drives a worm gear through a worm to achieve plane positioning, and the Z-axis feeding assembly controls vertical displacement through a second threaded rod penetrating through an inner thread of an assembling block; the cutting mechanism comprises a blade driven by two groups of belt pulleys and an assembling shell; the collecting mechanism comprises a rotatable turret and a plurality of collecting tanks. The submillimeter-level positioning precision of the three-dimensional adjusting mechanism (X / Y / Z axis) is set, precutting and the main blade work cooperatively, uniform slice thickness and high integrity are ensured, and through reasonable structural design, accurate and efficient sampling of tissue samples is achieved, manual operation errors are reduced, and the sample quality and the reliability of detection results are improved.

Description

Technical Field

[0001] The present invention specifically relates to a cell slice sampling device, belonging to the technical field of biomedical detection equipment. Background Art

[0002] Cell section sampling is a fundamental and crucial procedure in modern medical testing and biological research. Currently, traditional cell sampling methods rely heavily on manual manipulation, such as using ordinary sampling needles or blades for cutting and sampling. This approach has numerous drawbacks: Firstly, manual manipulation makes it difficult to precisely control the depth, angle, and range of the sample, which can easily lead to sampling errors and affect the accuracy of subsequent test results. Secondly, manual manipulation can cause unnecessary damage to the sample tissue due to hand tremors or uneven application of force, even leading to sample contamination.

[0003] While some existing sampling devices incorporate mechanical mechanisms to aid sampling, they often suffer from complex structures, awkward operation, and low sampling efficiency. For example, some devices lack flexible sampling heads, making them inflexible and unable to accommodate tissue samples of varying locations and morphologies. Others lack effective sample retention and collection mechanisms, making it easy for samples to be lost or mixed up. Furthermore, existing devices struggle to accurately and completely sample small or fragile tissue samples.

[0004] Therefore, it is necessary to design a cell slice sampling device with reasonable structure, convenient operation and accurate sampling to solve the problems existing in the prior art. Summary of the Invention

[0005] The purpose of the present invention is to address the deficiencies of the existing technology and provide a cell slice sampling device to achieve accurate and efficient sampling of tissue samples, reduce human operation errors, and improve sample quality and the reliability of test results.

[0006] A cell slice sampling device comprises: a sampling platform and a sample fixing mechanism arranged thereon; The three-dimensional adjustment mechanism includes an X / Y-axis translation assembly and a Z-axis feed assembly. The X / Y-axis translation assembly is driven by a worm gear to achieve planar positioning, and the Z-axis feed assembly controls vertical displacement through a No. 2 threaded rod that passes through the internal thread of the assembly block; a cutting mechanism comprising a blade driven by two sets of pulleys and an assembly housing; The collecting mechanism includes a rotatable turret and a plurality of collecting troughs.

[0007] Preferably, the handwheel drives the worm and worm gear meshing mechanism, the shell at the top of the sampling platform is connected with the No. 1 screw thread, and the X / Y axis translation is achieved through the No. 1 screw, No. 1 threaded rod and No. 1 threaded barrel.

[0008] Preferably, the blade forms an adjustable speed transmission system through a No. 1 pulley, a No. 2 pulley and a connecting belt.

[0009] Preferably, two groups of support plates are fixedly provided at the top of the sampling table compared to one side of the shell, and a second screw rod and a guide rod are rotatably provided inside the two groups of support plates, and positive and negative threads are provided on the outer side of the No. 2 screw rod, and two groups of clamping plates are provided through the outer threads of the No. 2 screw rod, and anti-slip silicone pads are provided on the inner sides of the two groups of clamping plates, and a tissue sample block is clamped between the two groups of clamping plates.

[0010] Preferably, eight groups of collecting slots are radially provided on the top of the turret of the collecting mechanism, and eight groups of protrusions are provided on the outer side of the turret.

[0011] Preferably, the pulley transmission system comprises: an interchangeable pulley set of different diameters, a first pulley and a second pulley, wherein the first pulley is connected to one side of the blade, and a crank is provided at one end of the second pulley.

[0012] Preferably, the device as a whole is modular in design, comprising: a detachable blade assembly; a replaceable collection turret; a quick-install belt drive module with pulley No. 1, pulley No. 2 and a connecting belt.

[0013] Preferably, a limiting plate is provided between the two groups of support plates, a pushing block is provided inside the limiting plate for movement, springs are provided on the outside of the two groups of rod bodies on one side of the pushing block, an "L"-shaped special-shaped plate is provided on the inside of the pushing block, a convex column is provided on the inside of the special-shaped plate, and the convex column can contact the convex block.

[0014] By adopting the above technical solution, during the rotation of the turret, the protrusion on the outside of the turret contacts the protrusion on the inside of the "L"-shaped special-shaped plate. As the turret rotates, the protrusion can be squeezed and pushed, so that the special-shaped plate drives the push block to move between the two groups of support plates.

[0015] Preferably, a mounting groove is provided inside the sampling platform, a filter is fixedly installed inside the mounting groove, and a fan is fixedly installed inside the mounting groove.

[0016] By adopting the above technical solution, the sampling table provides an installation space for the fan through the installation slot. The fan generates wind flow when it works, and then the outside air flows into the installation slot.

[0017] Preferably, a diversion trough is fixedly installed on the sampling table, and the diversion trough is located on one side of the turret, the fan output end is connected to the diversion trough through a pipeline, and an array of exhaust holes is arranged on the diversion trough, and an interception net for intercepting impurities is arranged inside the exhaust hole.

[0018] By adopting the above technical solution, the outside air will flow into the installation slot, and the filter will intercept impurities carried by the air, thereby improving the cleanliness of the air.

[0019] Preferably, a base plate is provided at the bottom of the sampling platform, a driving motor is fixedly installed at the bottom of the base plate, a threaded screw is fixedly connected to the output end of the driving motor, a No. 2 threaded barrel corresponding to the threaded screw is fixedly installed inside the sampling platform, and the threaded screw is threadedly connected to the No. 2 threaded barrel, a guide barrel is fixedly installed inside the sampling platform, a guide rod is passed through the inside of the guide barrel, and one end of the guide rod is fixedly connected to the base plate.

[0020] By adopting the above technical solution, the driving motor will drive the threaded screw to rotate, and when the threaded screw rotates, the No. 2 threaded barrel will move. When the No. 2 threaded barrel moves, it will drive the sampling platform to move. When the sampling platform moves, it will drive the guide barrel to slide on the guide rod, and then the height of the sampling platform will be adjusted.

[0021] 1. The present invention achieves submillimeter positioning accuracy by providing a three-dimensional adjustment mechanism (X / Y / Z axes). This mechanism works in conjunction with the pre-cutting blade and the main blade to ensure uniform slice thickness and high integrity. Through reasonable structural design, the device can accurately and efficiently sample tissue samples, significantly reducing errors caused by human operation. The system also improves sample quality and enhances the reliability of test results. It is suitable for various experimental and analytical scenarios requiring high-precision and high-consistency samples. It not only improves sampling efficiency but also provides a more reliable data foundation for subsequent experiments and research.

[0022] 2. The present invention greatly improves the efficiency of slicing operations by setting up a turret multi-station switching system and adopting eight groups of collection slots in a radial layout. After completing a slicing operation, the user only needs to manually rotate the turret, and the limiting structure of the protrusion and the protrusion will automatically cooperate to position, ensuring that the slices fall accurately into the designated slots in a predetermined order, effectively preventing cross contamination. The isolated collection slots are used to independently store samples, avoiding direct contact between different slices, not only realizing the continuous slicing process, but also significantly saving the time required to replace the collection container, providing users with a convenient and efficient operation experience. Through this improvement, the integrity and reliability of the samples are enhanced, the slicing process becomes more efficient, adapting to various complex experimental needs, and providing a solid foundation for subsequent analysis and research.

[0023] 3. The present invention sets a wind flow generated by the operation of the fan, and the wind enters the inside of the diversion trough, and then guides the wind to the surface of the turret through the exhaust hole, thereby effectively removing residual impurities on the surface of the turret and improving the cleanliness of the turret.

[0024] When the height of the sampling platform is adjusted according to actual needs, the present invention causes the driving motor to operate, which drives the screw to rotate. When the screw rotates, the second screw cylinder moves. When the second screw cylinder moves, it drives the sampling platform to move. When the sampling platform moves, it drives the guide cylinder to slide on the guide rod, thereby adjusting the height of the sampling platform. This facilitates the adjustment of the height of the sampling platform, improves flexibility, and facilitates use by people of different heights. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the shell shear structure of the present invention; Figure 3 It is a schematic diagram of the turret structure of the present invention; Figure 4 This is a schematic diagram of the bottom structure of the support plate of the present invention; Figure 5 For the present invention Figure 2 Schematic diagram of the local structure enlarged at point A; Figure 6 This is a schematic diagram of the mounting slot structure of the present invention; Figure 7 Schematic diagram of the diversion trough structure in the present invention; Figure 8 Schematic diagram of the bottom plate structure of the present invention.

[0026] In the figure: 1. sampling platform; 2. housing; 3. screw rod No. 1; 4. worm; 5. worm gear; 6. threaded rod No. 1; 7. threaded barrel No. 1; 8. assembly block; 9. threaded rod No. 2; 10. assembly housing; 11. blade; 12. pulley No. 1; 13. pulley No. 2; 14. connecting belt; 15. support plate; 16. screw rod No. 2; 17. clamping plate; 18. tissue sample block; 19. limit plate; 20. push block; 21. spring; 22. special-shaped plate; 23. boss; 24. turret; 25. boss; 26. collecting trough; 27. mounting trough; 28. fan; 29. ​​filter; 30. diverter trough; 31. exhaust hole; 32. bottom plate; 33. drive motor; 34. screw rod; 35. threaded barrel No. 2; 36. guide barrel; 37. guide rod. DETAILED DESCRIPTION

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0028] See also Figure 1-8 As shown, a cell slice sampling device; It includes: a sampling platform 1 and a sample fixing mechanism arranged thereon; The three-dimensional adjustment mechanism includes an X / Y-axis translation assembly and a Z-axis feed assembly. The X / Y-axis translation assembly is driven by a worm 4 to drive a worm wheel 5 to achieve planar positioning; the Z-axis feed assembly is controlled by a No. 2 threaded rod 9 in the assembly block 8 for vertical displacement, and the No. 2 threaded rod 9 passes through the threaded opening. The handwheel-driven worm 4 and the worm wheel 5 form a meshing mechanism. The top of the sampling table 1 is threadedly connected to the shell 2 by the No. 1 screw 3, and the No. 1 screw 3, the No. 1 threaded rod 6 and the No. 1 threaded barrel 7 realize the X / Y-axis translation function; The cutting mechanism includes a blade 11 driven by two sets of pulleys and an assembly shell 10. The blade 11 is driven by a first pulley 12, a second pulley 13 and a connecting belt 14 to form an adjustable speed transmission system; The collecting mechanism includes a rotatable turret 24 and a plurality of collecting slots 26 . Eight groups of collecting slots 26 are radially provided on the top of the turret 24 of the collecting mechanism, and eight groups of protrusions 25 are provided on the outer side of the turret 24 .

[0029] Two sets of support plates 15 are fixedly provided at the top of the sampling table 1 compared to one side of the shell 2. A second screw rod 16 and a guide rod are rotatably provided inside the two sets of support plates 15. The outer side of the second screw rod 16 is provided with positive and negative threads. Two sets of clamping plates 17 are provided through the outer threads of the second screw rod 16. The inner sides of the two sets of clamping plates 17 are provided with anti-slip silicone pads. A tissue sample block 18 is clamped between the two sets of clamping plates 17.

[0030] The pulley drive system includes: an interchangeable different-diameter pulley group No. 1 pulley 12 and a No. 2 pulley 13. The No. 1 pulley 12 is connected to one side of the blade 11, and a crank is provided at one end of the No. 2 pulley 13. The overall device is modular in design and includes: a detachable blade 11 assembly; a replaceable collection turret 24; a quick-install belt drive module No. 1 pulley 12, No. 2 pulley 13 and a connecting belt 14.

[0031] A limit plate 19 is arranged between the two groups of support plates 15, and a push block 20 is arranged inside the limit plate 19 for movement. A spring 21 is arranged on the outside of the two groups of rod bodies on one side of the push block 20, and an "L"-shaped special-shaped plate 22 is arranged on the inside of the push block 20. A convex column 23 is arranged on the inner side of the special-shaped plate 22, and the convex column 23 can contact the convex block 25.

[0032] Double-thread screw synchronous drive: The two sets of clamping plates 17 are driven to move synchronously toward or away from each other by the second screw 16 with positive and negative threads, ensuring that the tissue sample block 18 is evenly pressed between the two sets of support plates 15 to avoid deformation or damage of the sample caused by unilateral force. The anti-slip silicone pads on the inner side of the two sets of clamping plates 17 are used to increase the clamping friction to prevent the sample from sliding, while buffering the mechanical pressure and protecting the fragile tissue. The limit plate 19 limits the lateral displacement of the sample and cooperates with the clamping plate (17) to form a multi-directional fixation to adapt to samples of different shapes. The limit plate 19 is used to push The block 20 is guided to move in a straight line. When the turret 24 rotates, the protrusion 25 on the outside of the turret 24 contacts the protrusion 23 on the inside of the "L"-shaped special-shaped plate 22. As the turret 24 rotates, the protrusion 23 is squeezed and moved, so that the special-shaped plate 22 drives the pushing block 20 to move between the two sets of support plates 15. The pushing block 20 can be located below the tissue sample block 18. When the protrusion 25 is misaligned with the protrusion 23, the spring 21 on the rod outside the pushing block 20 elastically squeezes the pushing block 20, and the slice can be pushed into the corresponding collection groove 26. The turret 24 is radially designed with eight collection slots 26. After each slice, the user can manually rotate the turret 24 to ensure that the protrusions 25 and the limiting structures of the protrusions 23 precisely match to achieve accurate positioning. This design ensures that slices can fall into the designated slots in order, effectively improving the convenience of operation and the neatness of the slices. In order to prevent cross contamination, an isolated collection tank 26 is designed to store samples independently, effectively avoiding contact between different slices and ensuring the purity and reliability of the samples.

[0033] X / Y axis translation: The hand wheel drives the worm 4 to engage with the worm wheel 5, driving the No. 1 threaded rod (6) to rotate inside the No. 1 threaded barrel 7. As the No. 1 threaded barrel 7 gradually extends, the assembly block 8 can be moved linearly (the No. 1 threaded barrel 7 moves through the interior of the housing 2). The worm wheel 5 and the worm 4 have self-locking properties to prevent position displacement after operation. The No. 1 screw rod 3 is connected to the housing 2 through a thread. When the hand wheel at one end of the No. 1 screw rod 3 is rotated, the entire housing 2 can be translated.

[0034] Z-axis feed: The No. 2 threaded rod 9 is connected to the assembly block 8 through a thread. The No. 2 threaded rod 9 in the assembly block 8 can control the vertical feed of the blade 11. The profile design ensures that the feed amount is controllable.

[0035] Pulley speed transmission: By replacing the different diameter belt pulleys 12 and 13, the speed of the blade 11 can be adjusted to meet the cutting requirements of tissues with different hardness. The hand-cranked drive provides power-free operation, which is suitable for laboratory environments.

[0036] Modular blade 11 assembly: Blade 11 can be quickly disassembled and replaced to meet slicing needs of different thicknesses or materials.

[0037] The sampling platform 1 is provided with a bottom plate 32 at the bottom, a driving motor 33 is fixedly installed at the bottom of the bottom plate 32, and a threaded screw 34 is fixedly connected to the output end of the driving motor 33. A second threaded cylinder 35 corresponding to the threaded screw 34 is fixedly installed inside the sampling platform 1, and the threaded screw 34 is threadedly connected to the second threaded cylinder 35. A guide cylinder 36 is fixedly installed inside the sampling platform 1, and a guide rod 37 is passed through the guide cylinder 36, and one end of the guide rod 37 is fixedly connected to the bottom plate 32. The cell slice sampling device is used to sample cells. When slicing and sampling, the height of the sampling platform 1 is adjusted according to actual needs. When the driving motor 33 is working, the threaded screw 34 will be driven to rotate. When the threaded screw 34 rotates, the No. 2 threaded cylinder 35 will move. When the No. 2 threaded cylinder 35 moves, it will drive the sampling platform 1 to move. When the sampling platform 1 moves, it will drive the guide cylinder 36 to slide on the guide rod 37, and then the height of the sampling platform 1 will be adjusted, thereby achieving the purpose of facilitating the height adjustment of the sampling platform 1 for use, improving flexibility, and facilitating use by people of different heights.

[0038] The sampling platform 1 is provided with a mounting groove 27 inside, a filter screen 29 is fixedly installed inside the mounting groove 27, a fan 28 is fixedly installed inside the mounting groove 27, a diverter groove 30 is fixedly installed on the sampling platform 1, and the diverter groove 30 is located on one side of the turret 24, the output end of the fan 28 is connected to the diverter groove 30 through a pipeline, an array of exhaust holes 31 are arranged on the diverter groove 30, and an interception net for intercepting impurities is arranged inside the exhaust hole 31. The sampling platform 1 provides an installation space for the fan 28 through the mounting groove 27. The fan 28 generates wind flow when it is working, and then the outside air will flow into the mounting groove 27, and the filter screen 29 will intercept the impurities carried by the air. The fan 28 generates wind flow when it is working, and the wind flow enters the diverter groove 30. The wind will flow to the surface of the turret 24 through the exhaust hole 31, and then the impurities remaining on the surface of the turret 24 can be removed, thereby improving the cleanliness of the turret 24.

[0039] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0040] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A cell slice sampling device, characterized in that: include: A sampling platform (1) and a sample fixing mechanism provided thereon; A three-dimensional adjustment mechanism comprises an X / Y axis translation assembly and a Z axis feed assembly, wherein the X / Y axis translation assembly is driven by a worm (4) to drive a worm wheel (5) to achieve plane positioning, and the Z axis feed assembly is controlled by a second threaded rod (9) through which the internal thread of the assembly block (8) passes; A cutting mechanism comprising a blade (11) driven by two sets of pulleys and an assembly housing (10); The collecting mechanism comprises a rotatable turret (24) and a plurality of collecting troughs (26).

2. The cell slice sampling device according to claim 1, wherein: A handwheel-driven worm (4) and a worm gear (5) meshing mechanism are provided, wherein the housing (2) at the top of the sampling platform (1) is threadedly connected to the No. 1 screw rod (3), and the No. 1 threaded rod (6) and the No. 1 threaded barrel (7) are used to realize X / Y axis translation.

3. The cell slice sampling device according to claim 1, wherein: The blade (11) forms an adjustable speed transmission system through a No. 1 pulley (12), a No. 2 pulley (13) and a connecting belt (14).

4. The cell slice sampling device according to claim 1, wherein: Two groups of support plates (15) are fixedly provided at the top of the sampling table (1) relative to one side of the shell (2), and a second screw rod (16) and a guide rod are rotatably provided inside the two groups of support plates (15). The outer side of the second screw rod (16) is provided with positive and negative threads, and the outer threads of the second screw rod (16) are penetrated by two groups of clamping plates (17). The inner sides of the two groups of clamping plates (17) are provided with anti-slip silicone pads, and a tissue sample block (18) is clamped between the two groups of clamping plates (17).

5. The cell slice sampling device according to claim 1, wherein: Eight groups of collecting slots (26) are radially provided on the top of the turret (24) of the collecting mechanism, and eight groups of protrusions (25) are provided on the outer side of the turret (24).

6. The cell slice sampling device according to claim 1, wherein: The pulley transmission system comprises: an interchangeable pulley group of different-diameter pulleys, a first pulley (12) and a second pulley (13); the first pulley (12) is connected to one side of the blade (11); and a crank is provided at one end of the second pulley (13).

7. The cell slice sampling device according to claim 1, wherein: The device is modular in design and comprises: a detachable blade (11) assembly; a replaceable collection turret (24); a quick-install belt drive module, a first pulley (12), a second pulley (13) and a connecting belt (14).

8. The cell slice sampling device according to claim 4, wherein: A limiting plate (19) is provided between the two groups of support plates 15. A pushing block (20) is provided inside the limiting plate (19) so as to be movable and penetrate the inside of the limiting plate (19). A spring (21) is provided on the outside of the two groups of rod bodies on one side of the pushing block (20). An "L"-shaped special-shaped plate (22) is provided on the inside of the pushing block (20). A convex column (23) is provided on the inside of the special-shaped plate (22), and the convex column (23) can contact the convex block (25).

9. The cell slice sampling device according to claim 1, wherein: The sampling platform (1) is provided with an installation groove (27) inside, a filter screen (29) is fixedly installed inside the installation groove (27), a fan (28) is fixedly installed inside the installation groove (27), a diversion groove (30) is fixedly installed on the sampling platform (1), and the diversion groove (30) is located on one side of the turret (24), the output end of the fan (28) is connected to the diversion groove (30) through a pipeline, and an array of exhaust holes (31) are provided on the diversion groove (30), and an interception net for intercepting impurities is provided inside the exhaust hole (31).

10. The cell slice sampling device according to claim 9, wherein: The sampling platform (1) is provided with a bottom plate (32) at the bottom, a driving motor (33) is fixedly installed at the bottom of the bottom plate (32), and a threaded screw (34) is fixedly connected to the output end of the driving motor (33). A second threaded barrel (35) corresponding to the threaded screw (34) is fixedly installed inside the sampling platform (1), and the threaded screw (34) is threadedly connected to the second threaded barrel (35). A guide barrel (36) is fixedly installed inside the sampling platform (1), and a guide rod (37) is passed through the inside of the guide barrel (36), and one end of the guide rod (37) is fixedly connected to the bottom plate (32).

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