Ginseng hydrolat with anti-aging, anti-oxidation and oil-control effects as well as preparation method and application of ginseng hydrolat

Abstract

The invention relates to ginseng hydrolat with anti-aging, anti-oxidation and oil-control effects as well as a preparation method and application of the ginseng hydrolat. The preparation method comprises the following steps: mixing and soaking a ginseng raw material and an aqueous solution containing an additive in an oxygen-free environment, then carrying out steam distillation, collecting a distillate, and separating a water phase in the distillate to obtain the ginseng hydrolat, the additive comprises vitamin E, tea polyphenol and cyclodextrin. According to the ginseng hydrolat prepared by the method, various functional components in ginseng are obtained, and the ginseng hydrolat has excellent anti-aging, anti-oxidation and oil-control effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a ginseng hydrosol with anti-aging, anti-oxidation and oil-control effects, and a preparation method and application thereof. Background Art

[0002] Ginseng hydrosol has a variety of skin care benefits in cosmetics, mainly due to the active ingredients in ginseng (such as ginsenosides, polysaccharides, amino acids, trace elements, etc.). The use of ginseng hydrosol in cosmetics is mainly due to its moisturizing, skin brightening, anti-inflammatory and soothing effects, and antioxidant protection. Its application in cosmetics is mainly as a moisturizer and skin conditioner, providing moisturizing and repairing effects for the skin. However, due to the differences in the extraction methods of ginseng hydrosol, the skin care effects of ginseng hydrosol vary greatly, and the product application is also limited.

[0003] CN111317687A discloses a method for producing cosmetics using ginseng fruit enzymes and a preparation process thereof. The required raw materials include: 1.0g of ginseng total saponins with a purity of 85% or more, 50.0g of medical glycerin, 10.0ml of 70% pure ethanol, and an appropriate amount of distilled (deionized) water; these raw materials are mixed to form a 100ml mixed solution. This method, by adding ginseng fruit enzymes to cosmetics, achieves good solubility and facilitates their inclusion in various cosmetics without altering the properties of the ginseng fruit enzymes or other ingredients. The enzymes can utilize the advantages of the original ingredients while also utilizing the wrinkle-removing, whitening, and hydrating properties of the ginseng fruit enzymes.

[0004] CN109528521A discloses a method for producing anti-aging cosmetics using ginseng. The method comprises adding an appropriate amount of deionized water to ginseng powder at a solid-liquid ratio of 1:5-1:10, mixing the mixture evenly, and subjecting the mixture to distillation extraction at 50-70°C and a vacuum degree of 0.06-0.07 MPa. When the volume of the distillate is half the amount of water added, the distillation is stopped to produce a ginseng extract.

[0005] However, the products extracted by the preparation methods disclosed in the prior art are either too simple and difficult to meet the multi-dimensional efficacy requirements of cosmetics; or the products are too complex, with a low proportion of effective active substances and serious impurity interference, making it impossible to fully realize the application value of the active ingredients.

[0006] Therefore, developing a preparation process for ginseng pure water based on the multiple active ingredients in ginseng, which can obtain the effective ingredients in ginseng to a greater extent, has become one of the technical problems that need to be solved urgently. Summary of the Invention

[0007] In response to the shortcomings of the prior art, the present invention aims to provide a ginseng hydrosol with anti-aging, antioxidant, and oil-control effects, as well as a preparation method and application thereof. The ginseng hydrosol prepared by this method extracts multiple active ingredients from ginseng and has excellent anti-aging, antioxidant, and oil-control effects.

[0008] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a method for preparing a ginseng hydrosol having anti-aging, antioxidant, and oil-control effects, the preparation method comprising the following steps:

[0010] The ginseng raw material and the aqueous solution containing additives are mixed and soaked in an oxygen-free environment, and then steam distilled, the distillate is collected, and the aqueous phase in the distillate is separated to obtain ginseng pure water.

[0011] The additives include vitamin E, tea polyphenols and cyclodextrin.

[0012] The present invention extracts the effective components in ginseng by steam distillation. Meanwhile, the ginseng raw material is soaked in an aqueous solution containing additives, which can greatly reduce the oxidation loss of heat-sensitive effective components in ginseng during the distillation process.

[0013] Vitamin E, as a fat-soluble antioxidant, can effectively protect the active ingredients in ginseng, such as saponins; tea polyphenols refer to phenolic compounds in tea and are an excellent natural antioxidant; cyclodextrin is a cyclic oligosaccharide that can also inhibit the oxidation of heat-sensitive active ingredients in ginseng.

[0014] The present invention innovatively discovered that vitamin E, tea polyphenols, and cyclodextrin have a significant synergistic effect in protecting the heat-sensitive active ingredients in ginseng. The simultaneous addition of vitamin E, tea polyphenols, and cyclodextrin significantly enhances the anti-aging, antioxidant, and oil-control effects of the resulting ginseng hydrosol.

[0015] Preferably, the cyclodextrin includes any one of α-cyclodextrin, β-cyclodextrin or γ-cyclodextrin, or a combination of at least two of them, preferably β-cyclodextrin.

[0016] Preferably, the mass ratio of vitamin E, tea polyphenols and cyclodextrin is (0.5-1):(0.1-0.5):(0.5-1).

[0017] Among them, the specific point values ​​in 0.5-1 can be selected from 0.5, 0.6, 0.7, 0.8, 0.9, 1, etc., and the specific point values ​​in 0.1-0.5 can be selected from 0.1, 0.2, 0.3, 0.4, 0.5, etc.

[0018] Preferably, the mass percentage of the additive in the aqueous solution containing the additive is 0.1-0.5%, for example, it can be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, etc.

[0019] Preferably, the mass volume ratio of ginseng to the aqueous solution containing additives is 1:(3-5) g / mL, for example, it can be 1:3 g / mL, 1:3.5 g / mL, 1:4 g / mL, 1:4.5 g / mL, 1:5 g / mL, etc.

[0020] Preferably, the temperature of the mixed soaking is 20-40° C., and the time of the mixed soaking is 1-4 hours.

[0021] Among them, the specific point values ​​in 20-40℃ can be selected from 20℃, 25℃, 30℃, 35℃, 40℃, etc., and the specific point values ​​in 1-4h can be selected from 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, etc.

[0022] Preferably, the ginseng raw material includes ginseng root raw material and ginseng flower raw material.

[0023] The present invention creatively discovers that by using ginseng roots and ginseng flowers as reaction raw materials, the two cooperate with each other and synergistically enhance the effect, and the ginseng pure water prepared has more excellent anti-aging, antioxidant and oil-control effects.

[0024] Preferably, the mass ratio of the ginseng root raw material to the ginseng flower raw material is (2-10):1, for example, it can be 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, etc.

[0025] Preferably, the steam distillation is performed in a staged distillation manner, and then the distillates obtained in each stage are mixed;

[0026] The segmented distillation comprises the following steps:

[0027] (I) Distill at 60-65°C for 30-45 minutes to obtain distillate I.

[0028] (II) Distill at 80-85°C for 2-3 hours to obtain distillate II.

[0029] (III) Distill at 88-92°C for 1-5 min to obtain distillate III.

[0030] Among them, the specific point values ​​in 60-65℃ can be selected from 60℃, 61℃, 62℃, 63℃, 64℃, 65℃, etc., the specific point values ​​in 80-85℃ can be selected from 80℃, 81℃, 82℃, 83℃, 84℃, 85℃, etc., the specific point values ​​in 88-92℃ can be selected from 88℃, 89℃, 90℃, 91℃, 92℃, etc., the specific point values ​​in 30-45min can be selected from 30min, 33min, 36min, 39min, 42min, 45min, etc., the specific point values ​​in 2-3h can be selected from 2h, 2.2h, 2.4h, 2.6h, 2.8h, 3h, etc., and the specific point values ​​in 1-5min can be selected from 1min, 2min, 3min, 4min, 5min, etc.

[0031] The present invention collects three parts of distillate through three-stage distillation based on functional ingredients such as saponins and polysaccharides, and the prepared ginseng pure water has richer and higher content of anti-aging, antioxidant and oil-control functional ingredients.

[0032] Preferably, the distillation pressure in step (I) is 0.05-0.07 MPa, for example, it can be 0.05 MPa, 0.055 MPa, 0.06 MPa, 0.065 MPa, or 0.07 MPa.

[0033] Preferably, the distillation pressure in step (II) is 0.08-0.09 MPa, for example, it can be 0.08 MPa, 0.083 MPa, 0.086 MPa, or 0.09 MPa.

[0034] Preferably, the distillation pressure in step (III) is 0.1-0.12 MPa, for example, 0.1 MPa, 0.105 MPa, 0.11 MPa, 0.115 MPa, or 0.12 MPa.

[0035] Preferably, after separating the aqueous phase from the distillate, the method further comprises filtering the separated aqueous phase to collect a filtrate having a membrane cut-off molecular weight of 500-2000 Da.

[0036] Among them, specific point values ​​in 500-2000Da can be selected from 500Da, 1000Da, 1500Da, 2000Da, etc.

[0037] Preferably, the filtration further comprises vacuum concentrating the filtrate.

[0038] Preferably, the temperature of the vacuum concentration is 40-50°C, for example, 40°C, 42°C, 44°C, 46°C, 48°C, 50°C, etc.

[0039] In a second aspect, the present invention provides a ginseng hydrosol prepared by the preparation method of the ginseng hydrosol with anti-aging, antioxidant and oil-control effects as described in the first aspect.

[0040] In a third aspect, the present invention provides a use of the ginseng hydrosol as described in the second aspect in cosmetics.

[0041] The numerical range described in the present invention includes not only the point values ​​listed above, but also any point values ​​between the above numerical ranges that are not listed. Due to space limitations and for the sake of simplicity, the present invention no longer exhaustively lists the specific point values ​​included in the range.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] The present invention extracts the active ingredients from ginseng through steam distillation. Simultaneously, the ginseng raw material is soaked in an aqueous solution containing additives, significantly reducing the oxidative loss of heat-sensitive active ingredients in ginseng during the distillation process. The additives, vitamin E, tea polyphenols, and cyclodextrin, exhibit a significant synergistic effect in protecting the heat-sensitive active ingredients in ginseng. The simultaneous addition of vitamin E, tea polyphenols, and cyclodextrin significantly enhances the anti-aging, antioxidant, and oil-control effects of the resulting ginseng hydrosol.

[0044] Furthermore, ginseng root and ginseng flower are used as reaction raw materials together, and the two cooperate with each other to synergistically enhance the effect, and the prepared ginseng pure water has more excellent anti-aging, antioxidant and oil-control effects.

[0045] Furthermore, the present invention collects three parts of distillate through three-stage distillation, which makes up for the shortcomings of traditional plant extracts in antioxidant and repair effects. Through segmented collection, different functional components are accurately separated, the quality of the pure water is improved, and the prepared ginseng pure water has richer and higher content of anti-aging, antioxidant and oil-control functional ingredients. DETAILED DESCRIPTION

[0046] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0047] The sources of various raw materials in the following specific embodiments are shown in Table 1.

[0048] Table 1

[0049]

[0050]

[0051] Example 1

[0052] This embodiment provides a method for preparing ginseng hydrosol, comprising the following steps:

[0053] (1) Fresh ginseng roots and ginseng flowers (mass ratio of 4:1) were minced and mixed, and an aqueous solution containing 0.3% of additives (the additives were vitamin E, tea polyphenols, and β-cyclodextrin in a mass ratio of 0.7:0.2:1) was added in a distillation kettle at a mass volume ratio of 1:4 g / mL. At the same time, N2 was introduced to replace the air, and the mixture was soaked at 30°C for 2 h.

[0054] (2) Use temperature-controlled equipment for segmented distillation and use multi-stage spiral condensers (5°C cold water circulation) to ensure rapid condensation of steam. Segmented distillation is:

[0055] ① Slowly increase the temperature to 62℃ (pressure 0.06MPa), maintain for 35min, and collect the first fraction (distillate I); ② Increase the temperature to 82℃ (pressure 0.08MPa), maintain for 3h, and collect the main fraction (distillate II); ③ Increase the temperature to 90℃ (pressure 0.11MPa), maintain for 3min, and collect the tail fraction (distillate III).

[0056] The three distillates were mixed, allowed to stand at 4°C for 24 h, and separated using a separatory funnel to obtain the aqueous phase.

[0057] (3) The separated aqueous phase was passed through a 200-mesh sieve to remove suspended impurities, and then filtered (using a 10 kDa molecular weight cutoff membrane for ultrafiltration to remove large molecular substances, and then using a 1000 Da molecular weight cutoff membrane for nanofiltration to retain small molecular active substances); the collected filtrate was concentrated under vacuum at 45°C, the concentrate was freeze-dried, and re-dissolved with water to obtain a ginseng hydrosol with a solid content of 30%.

[0058] Example 2

[0059] This embodiment provides a method for preparing ginseng hydrosol, comprising the following steps:

[0060] (1) Fresh ginseng roots and ginseng flowers (mass ratio of 2:1) were minced and mixed, and an aqueous solution containing 0.5% additives (the additives were vitamin E, tea polyphenols, and β-cyclodextrin in a mass ratio of 0.5:0.1:0.7) was added to a distillation kettle at a mass volume ratio of 1:3 g / mL. At the same time, N2 was introduced to replace the air, and the mixture was soaked at 20°C for 4 h.

[0061] (2) Use temperature-controlled equipment for segmented distillation and use multi-stage spiral condensers (8°C cold water circulation) to ensure rapid condensation of steam. Segmented distillation is:

[0062] ① Slowly increase the temperature to 60℃ (pressure 0.05MPa), maintain for 45min, and collect the first fraction (distillate I); ② Increase the temperature to 80℃ (pressure 0.08MPa), maintain for 2h, and collect the main fraction (distillate II); ③ Increase the temperature to 88℃ (pressure 0.1MPa), maintain for 5min, and collect the tail fraction (distillate III).

[0063] The three distillates were mixed, allowed to stand at 4°C for 20 h, and separated using a separatory funnel to obtain the aqueous phase.

[0064] (3) The separated aqueous phase was passed through a 200-mesh sieve to remove suspended impurities, and then filtered (using a 10 kDa molecular weight cutoff membrane for ultrafiltration to remove large molecular substances, and then using a 2000 Da molecular weight cutoff membrane for nanofiltration to retain small molecular active substances); the collected filtrate was concentrated under vacuum at 40° C., the concentrate was freeze-dried, and re-dissolved with water to obtain a ginseng hydrosol with a solid content of 30%.

[0065] Example 3

[0066] This embodiment provides a method for preparing ginseng hydrosol, comprising the following steps:

[0067] (1) Fresh ginseng roots and ginseng flowers (mass ratio of 10:1) were minced and mixed, and an aqueous solution containing 0.1% additives (the additives of vitamin E, tea polyphenols and β-cyclodextrin were vitamin E, tea polyphenols and β-cyclodextrin at a mass ratio of 1:5 g / mL) was added to a distillation kettle at a mass volume ratio of 1:0.5:0.5. At the same time, N2 was introduced to replace the air, and the mixture was soaked at 40°C for 1 h.

[0068] (2) Use temperature-controlled equipment for segmented distillation and use multi-stage spiral condensers (10°C cold water circulation) to ensure rapid condensation of steam. Segmented distillation is:

[0069] ① Slowly increase the temperature to 65℃ (pressure 0.07MPa), maintain for 30min, and collect the first fraction (distillate I); ② Increase the temperature to 85℃ (pressure 0.09MPa), maintain for 3h, and collect the main fraction (distillate II); ③ Increase the temperature to 92℃ (pressure 0.12MPa), maintain for 1min, and collect the tail fraction (distillate III).

[0070] The three distillates were mixed, allowed to stand at 4°C for 22 h, and separated using a separatory funnel to obtain the aqueous phase.

[0071] (3) The separated aqueous phase was passed through a 200-mesh sieve to remove suspended impurities, and then filtered (a 10 kDa molecular weight cutoff membrane was used for ultrafiltration to remove large molecular substances, and then a 500 Da molecular weight cutoff membrane was used for nanofiltration to retain small molecular active substances); the collected filtrate was concentrated under vacuum at 50° C., the concentrate was freeze-dried, and re-dissolved with water to obtain a ginseng hydrosol with a solid content of 30%.

[0072] Example 4

[0073] This embodiment provides a method for preparing ginseng hydrosol, which differs from Example 1 only in that step (1) removes the ginseng flowers, i.e., fresh ginseng roots are chopped and an aqueous solution containing additives is added to a distillation kettle, and the remaining steps remain the same as Example 1.

[0074] Example 5

[0075] This embodiment provides a method for preparing ginseng hydrosol, which differs from Example 1 only in that step (1) removes the ginseng roots, i.e., chop the fresh ginseng flowers, and add an aqueous solution containing additives into a distillation kettle. The remaining steps remain the same as Example 1.

[0076] Example 6

[0077] This embodiment provides a method for preparing ginseng hydrosol, which differs from embodiment 1 only in that the mode of adjusting the segmented distillation in step (2) is as follows: ① slowly heating to 62°C (pressure 0.06 MPa), maintaining for 35 minutes, and collecting the first fraction (distillate I); ② heating to 82°C (pressure 0.08 MPa), maintaining for 3 hours, and collecting the main fraction (distillate II). The two fractions are mixed, allowed to stand at 4°C for 24 hours, and separated by a separatory funnel to obtain an aqueous phase. The remaining steps are consistent with embodiment 1.

[0078] Example 7

[0079] This embodiment provides a method for preparing ginseng hydrosol, which differs from embodiment 1 only in that the mode of adjusting the segmented distillation in step (2) is as follows: ① slowly heating to 62°C (pressure 0.06 MPa), maintaining for 35 minutes, and collecting the first fraction (distillate I); ② heating to 90°C (pressure 0.11 MPa), maintaining for 3 minutes, and collecting the last fraction (distillate III). The two fractions are mixed, allowed to stand at 4°C for 24 hours, and separated by a separatory funnel to obtain an aqueous phase. The remaining steps are consistent with embodiment 1.

[0080] Example 8

[0081] This embodiment provides a method for preparing ginseng hydrosol, which differs from embodiment 1 only in that the mode of adjusting the segmented distillation in step (2) is as follows:

[0082] ① Raise the temperature to 82°C (pressure 0.08 MPa), maintain for 3 hours, and collect the main fraction (distillate II); ② Raise the temperature to 90°C (pressure 0.11 MPa), maintain for 3 minutes, and collect the tail fraction (distillate III). Combine the two fractions, let stand at 4°C for 24 hours, and separate using a separatory funnel to obtain the aqueous phase. The remaining steps remain the same as in Example 1.

[0083] Example 9

[0084] This embodiment provides a method for preparing ginseng hydrosol, which differs from embodiment 1 only in that the mode of adjusting the segmented distillation in step (2) is as follows:

[0085] ① Slowly increase the temperature to 50℃ (pressure 0.06MPa), maintain for 35min, and collect the first fraction (distillate I); ② Increase the temperature to 70℃ (pressure 0.08MPa), maintain for 3h, and collect the main fraction (distillate II); ③ Increase the temperature to 85℃ (pressure 0.11MPa), maintain for 3min, and collect the tail fraction (distillate III).

[0086] The three fractions were mixed, allowed to stand at 4° C. for 24 h, and separated using a separatory funnel to obtain an aqueous phase. The remaining steps were the same as in Example 1.

[0087] Comparative Example 1

[0088] This comparative example provides a method for preparing ginseng hydrosol, which differs from Example 1 only in that, in step (1), the total mass of the additives is kept unchanged, and the additives are adjusted to vitamin E and tea polyphenols with a mass ratio of 0.7:0.2, and the remaining steps are consistent with Example 1.

[0089] Comparative Example 2

[0090] This comparative example provides a method for preparing ginseng hydrosol, which differs from Example 1 only in that, in step (1), the total mass of the additives is kept unchanged, and the additives are adjusted to vitamin E and β-cyclodextrin with a mass ratio of 0.7:1, and the remaining steps are consistent with Example 1.

[0091] Comparative Example 3

[0092] This comparative example provides a method for preparing ginseng hydrosol, which differs from Example 1 only in that, in step (1), the total mass of the additives is kept unchanged, and the additives are adjusted to tea polyphenols and β-cyclodextrin in a mass ratio of 0.2:1, and the remaining steps are consistent with Example 1.

[0093] Application Example 1

[0094] This application example provides an essence water, which includes, by mass percentage, 5% of the ginseng hydrosol prepared in Example 1, 5% of polydimethylsiloxane, 3% of squalane, 2% of isohexadecane, 3% of glycerin, 5% of butylene glycol, 3% of hyaluronic acid, 0.3% of carbomer, and the balance is water.

[0095] Application Example 2-9

[0096] Application Examples 2-9 each provide an essence water, which differs from Application Example 1 only in that the ginseng hydrosol prepared in Example 1 is replaced with the ginseng hydrosol prepared in Examples 2-9 in equal amounts, and the rest of the formula remains consistent with Application Example 1.

[0097] Comparative Application Examples 1-3

[0098] Comparative Application Examples 1-3 each provide an essence water, which differs from Application Example 1 only in that the ginseng hydrosol prepared in Example 1 is replaced by the ginseng hydrosol prepared in Comparative Examples 1-3 in equal mass, and the rest of the formula remains consistent with Application Example 1.

[0099] Test Example 1

[0100] Antioxidant capacity test.

[0101] (1) Evaluation of DPPH free radical scavenging ability:

[0102] (1.1) Principle:

[0103] DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) is commonly used to evaluate the in vitro antioxidant activity of antioxidants. DPPH is a stable nitrogen-centered free radical containing a single electron. It has a maximum light absorption at 517 nm, and its alcohol solution appears purple. In the presence of a free radical scavenger, the DPPH radical accepts an electron, causing its light absorption to gradually disappear, and the solution color changes from dark purple to yellow. The degree of discoloration is quantitatively related to the number of electrons accepted (i.e., free radical scavenging activity), allowing for rapid quantitative analysis using a spectrophotometer. Specifically, the stronger the free radical scavenger's scavenging ability, the lower the DPPH absorbance.

[0104] (1.2) Steps:

[0105] 1.0 mL of ginseng hydrosol (diluted 5-fold) prepared in each example and comparative example was placed in a 10 mL centrifuge tube. 3.0 mL of DPPH solution (0.008 mmol / L) was added. The mixture was reacted at room temperature in the dark for 30 minutes. The absorbance was measured at 517 nm using anhydrous ethanol as a blank. The DPPH radical scavenging rate was calculated according to the following formula.

[0106] DPPH free radical scavenging rate = A0-(As-Ac) / A0×100%;

[0107] Where A0 is the absorbance of 1.0 mL distilled water + 3.0 mL DPPH solution, As is the absorbance of 1.0 mL sample solution + 3.0 mL DPPH solution, and Ac is the absorbance of 1.0 mL sample solution + 3.0 mL anhydrous ethanol.

[0108] (2) Evaluation of ABST free radical scavenging ability:

[0109] (2.1) Principle:

[0110] ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) can be oxidized by K2S2O8 to produce blue-green ABTS + Free radical, with maximum light absorption at 734nm, ABTS + It is quite stable, but in the presence of antioxidants, ABTS + It reacts with it to produce colorless ABTS.

[0111] (2.2) Steps:

[0112] Take 0.1 mL of the ginseng hydrosol prepared in each example and comparative example (diluted 5-fold), add 0.1 mL of anhydrous ethanol, mix thoroughly, then add 0.8 mL of ABTS+ solution (0.1 mmol / L), mix thoroughly, and let stand for 6 minutes. Meanwhile, measure the absorbance at 734 nm using anhydrous ethanol as a blank. Calculate the ABTS free radical scavenging rate according to the following formula.

[0113] ABTS free radical scavenging rate = (A0-A0) / A0×100%;

[0114] Where A0 is the absorbance of 0.2 mL distilled water + 0.8 mL ABTS+ solution, and A is the absorbance of 0.2 mL sample solution + 0.8 mL ABTS+ solution.

[0115] The free radical scavenging ability of the ginseng hydrosols prepared in the examples and comparative examples is shown in Table 2.

[0116] Table 2

[0117]

[0118]

[0119] It can be seen from the test data in Table 2 that the ginseng hydrosol of the present invention has excellent DPPH free radical scavenging activity and ABTS free radical scavenging activity.

[0120] From the comparison of the data of Example 1 and Comparative Examples 1-3, it can be seen that vitamin E, tea polyphenols and cyclodextrin in the additives have a significant synergistic effect in protecting the heat-sensitive effective components in ginseng. Adding vitamin E, tea polyphenols and cyclodextrin at the same time can significantly enhance the antioxidant effect of the prepared ginseng hydrosol.

[0121] From the comparison of the data of Example 1 and Examples 4-5, it can be seen that by using ginseng roots and ginseng flowers as reaction raw materials, the two cooperate with each other and synergistically enhance the effect, and the ginseng pure water prepared has better antioxidant effect.

[0122] From the comparison of the data of Example 1 and Examples 6-9, it can be seen that the steps of the segmented distillation are set as follows: (Ⅰ) distillation at 60-65°C for 30-45min to obtain distillate I; (Ⅱ) distillation at 80-85°C for 2-3h to obtain distillate II; (Ⅲ) distillation at 88-92°C for 1-5min to obtain distillate III. This can accurately separate different functional components, improve the quality of the pure dew, and the prepared ginseng pure dew has richer and higher content of antioxidant functional ingredients.

[0123] Test Example 2

[0124] Anti-aging effect test:

[0125] 120 subjects aged 20-45 (60 males and 60 females) were randomly selected. The subjects presented with noticeable fine lines and wrinkles, accompanied by sagging, and were divided into 12 groups. After cleansing their skin, each group of subjects took an appropriate amount of the essence water product from Application Examples 1-9 or Comparative Application Examples 1-3 and evenly applied it to the entire face and eye area, gently massaging until completely absorbed. This was done once daily, morning and evening, for two months. Skin images were captured using the VISIA-CR facial image analyzer, and the wrinkle percentage was measured. The skin images were then captured and the crow's feet and wrinkle reduction rates were calculated.

[0126] Crow's feet reduction rate = (percentage of crow's feet before using the product - percentage of crow's feet after using the product) / percentage of crow's feet before using the product.

[0127] Nasal fold reduction rate = (nasolabial fold ratio before using the product - nasolabial fold ratio after using the product) / nasolabial fold ratio before using the product.

[0128] The test results are shown in Table 3.

[0129] Table 3

[0130] Group Crow's feet reduction rate Nasal fold reduction rate Application Example 1 28.5％ 29.1％ Application Example 2 27.8％ 26.4％ Application Example 3 26.2％ 25.7％ Application Example 4 22.3％ 21.9％ Application Example 5 19.6％ 18.4％ Application Example 6 24.1％ 20.8％ Application Example 7 18.9％ 17.4％ Application Example 8 21.5％ 19.6％ Application Example 9 16.8％ 15.3％ Comparative Application Example 1 13.4％ 12.7％ Comparative Application Example 2 11.2％ 10.8％ Comparative Application Example 3 14.6％ 13.9％

[0131] It can be seen from the test data in Table 3 that the ginseng hydrosol of the present invention has excellent anti-wrinkle and anti-aging effects.

[0132] From the comparison of the data of Application Example 1 and Comparative Application Examples 1-3, it can be seen that vitamin E, tea polyphenols and cyclodextrin in the additives have a significant synergistic effect in protecting the heat-sensitive effective components in ginseng. Adding vitamin E, tea polyphenols and cyclodextrin at the same time can significantly enhance the anti-wrinkle and anti-aging effects of the prepared ginseng hydrosol.

[0133] From the comparison of the data of Application Example 1 and Application Examples 4-5, it can be seen that by using ginseng roots and ginseng flowers as reaction raw materials, the two cooperate with each other and synergistically enhance the effect, and the ginseng pure water prepared has better anti-wrinkle and anti-aging effects.

[0134] From the comparison of the data of Application Example 1 and Application Examples 6-9, it can be seen that the steps of the segmented distillation are set as: (Ⅰ) distillation at 60-65℃ for 30-45min to obtain distillate I; (Ⅱ) distillation at 80-85℃ for 2-3h to obtain distillate II; (Ⅲ) distillation at 88-92℃ for 1-5min to obtain distillate III. This can accurately separate different effective components and improve the quality of the pure water. The prepared ginseng pure water has richer and higher content of anti-wrinkle and anti-aging effective ingredients.

[0135] Test Example 3

[0136] Oil control effect test:

[0137] 4g of each of the essences from Application Examples 1-9 and Comparative Application Examples 1-3 was evenly applied to a 60mm diameter glass Petri dish. The dish was then placed in a 30°C incubator for 48 hours, followed by 30 minutes at 25°C. 40μL of oleic acid was then dropped into the center of the dish. After 30 minutes, the area of ​​oleic acid spread was observed and the diameter of the oil stain was measured. Three groups were run for each product, and the average value was taken. The smaller the oil stain diameter, the stronger the product's oil-control ability. The test results for the essences from each application example and comparative application example are shown in Table 4.

[0138] Table 4

[0139] Group Oil spot diameter (mm) Application Example 1 7.2 Application Example 2 7.5 Application Example 3 7.8 Application Example 4 10.1 Application Example 5 12.6 Application Example 6 9.3 Application Example 7 14.8 Application Example 8 11.2 Application Example 9 15.5 Comparative Application Example 1 17.3 Comparative Application Example 2 18.5 Comparative Application Example 3 16.9

[0140] It can be seen from the test data in Table 4 that the ginseng hydrosol of the present invention can significantly reduce the diameter of oil spots and has an excellent oil control effect.

[0141] From the comparison of the data of Application Example 1 and Comparative Application Examples 1-3, it can be seen that vitamin E, tea polyphenols and cyclodextrin in the additives have a significant synergistic effect in protecting the heat-sensitive effective components in ginseng. Adding vitamin E, tea polyphenols and cyclodextrin at the same time can significantly improve the oil-control effect of the prepared ginseng hydrosol.

[0142] From the comparison of the data of Application Example 1 and Application Examples 4-5, it can be seen that by using ginseng roots and ginseng flowers as reaction raw materials, the two cooperate with each other and synergistically enhance the effect, and the ginseng pure water prepared has a better oil control effect.

[0143] From the data comparison between Application Example 1 and Application Examples 6-9, it can be seen that the steps of the segmented distillation are set as: (Ⅰ) distillation at 60-65℃ for 30-45min to obtain distillate I; (Ⅱ) distillation at 80-85℃ for 2-3h to obtain distillate II; (Ⅲ) distillation at 88-92℃ for 1-5min to obtain distillate III. This can accurately separate different functional components and improve the quality of the pure dew. The prepared ginseng pure dew has richer and higher content of oil-control functional ingredients.

[0144] The applicant declares that the present invention is illustrated by the above-described embodiments, but the present invention is not limited to the above-described embodiments. This does not mean that the present invention must rely on the above-described embodiments in order to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent replacements for the raw materials of the present invention, additions of auxiliary ingredients, and selection of specific methods are all within the scope of protection and disclosure of the present invention.

[0145] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0146] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A method for preparing ginseng hydrosol with anti-aging, anti-oxidation and oil-control effects, characterized in that: The preparation method comprises the following steps: The ginseng raw material and the aqueous solution containing additives are mixed and soaked in an oxygen-free environment, and then steam distilled, the distillate is collected, and the aqueous phase in the distillate is separated to obtain ginseng hydrosol; The additives include vitamin E, tea polyphenols and cyclodextrin.

2. The preparation method according to claim 1, characterized in that The cyclodextrin includes any one of α-cyclodextrin, β-cyclodextrin or γ-cyclodextrin or a combination of at least two thereof, preferably β-cyclodextrin; Preferably, the mass ratio of vitamin E, tea polyphenols and cyclodextrin is (0.5-1):(0.1-0.5):(0.5-1).

3. The preparation method according to claim 1 or 2, characterized in that The mass percentage of the additive in the aqueous solution containing the additive is 0.1-0.5%; Preferably, the mass volume ratio of the ginseng to the aqueous solution containing the additive is 1:(3-5) g / mL; Preferably, the temperature of the mixed soaking is 20-40° C., and the time of the mixed soaking is 1-4 hours.

4. The preparation method according to any one of claims 1 to 3, characterized in that The ginseng raw materials include ginseng root raw materials and ginseng flower raw materials; Preferably, the mass ratio of the ginseng root raw material to the ginseng flower raw material is (2-10):

1.

5. The preparation method according to any one of claims 1 to 4, characterized in that The steam distillation method is staged distillation, and then the distillates obtained in each stage are mixed; The segmented distillation comprises the following steps: (I) distilling at 60-65°C for 30-45 minutes to obtain distillate I; (II) distilling at 80-85°C for 2-3 hours to obtain distillate II; (III) Distill at 88-92°C for 1-5 min to obtain distillate III.

6. The preparation method according to claim 5, characterized in that The distillation pressure in step (I) is 0.05-0.07 MPa; Preferably, the distillation pressure in step (II) is 0.08-0.09 MPa; Preferably, the distillation pressure in step (III) is 0.1-0.12 MPa.

7. The preparation method according to any one of claims 1 to 6, characterized in that After separating the aqueous phase from the distillate, the method further comprises filtering the separated aqueous phase and collecting a filtrate with a membrane cut-off molecular weight of 500-2000 Da.

8. The preparation method according to claim 7, characterized in that After the filtration, the filtrate is further concentrated in vacuo; Preferably, the temperature of the vacuum concentration is 40-50°C.

9. Ginseng hydrosol prepared by the method for preparing ginseng hydrosol with anti-aging, antioxidant and oil-control effects according to any one of claims 1 to 8.

10. Use of the ginseng hydrosol according to claim 9 in cosmetics.

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