Exosome composition, method for promoting synthesis of collagen and application of exosome composition

Abstract

The invention discloses an exosome composition as well as a method and application thereof for promoting collagen synthesis, and particularly relates to the technical field of cosmetics, the exosome composition comprises the following components in parts by weight: 15-30 parts of human umbilical cord mesenchymal stem cell exosome, 8-15 parts of radix scutellariae extract, 2-6 parts of acetyl hexapeptide-2 and 1-4 parts of zinc gluconate, the composition can also comprise a humectant and a thickening agent. The preparation method comprises the following steps: preparing the human umbilical cord mesenchymal stem cell exosome, dissolving the radix scutellariae extract, the acetyl hexapeptide-8 and the zinc gluconate, mixing with the exosome, adding the humectant and the thickening agent, adjusting the pH value, and removing bacteria. Through the synergistic effect of all the components, the composition can effectively promote skin fibroblasts to synthesize type I collagen, has good anti-aging and anti-inflammatory effects, is high in safety and can be widely applied to preparation of anti-aging skin care products.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and more specifically, to an exosome composition and a method and application thereof for promoting collagen synthesis. Background Art

[0002] With the improvement of people's living standards and the pursuit of beauty, anti-aging skincare has become a hot topic. Skin aging is a complex physiological process, manifested primarily by decreased skin elasticity, increased wrinkles, and decreased collagen content. Collagen, particularly type I collagen, is a key component of the skin. Its content directly affects skin firmness and elasticity. Therefore, promoting collagen synthesis has become a key approach to anti-aging skincare.

[0003] Currently, there are many skincare products on the market claiming to have anti-aging effects, many of which contain ingredients that promote collagen synthesis. For example, some products use peptides to promote collagen synthesis by inhibiting muscle contraction or signal transduction, while others use plant extracts to utilize their antioxidant or anti-inflammatory properties to help improve skin condition.

[0004] However, most existing products have the following shortcomings: First, the effect of a single ingredient is limited, making it difficult to effectively promote collagen synthesis; second, some ingredients are less stable and easily affected by the external environment and lose their activity; third, some products may be irritating to the skin, and their safety needs to be improved; fourth, the synergistic effect between the ingredients is not obvious, and their respective functions cannot be fully exerted, making it difficult to achieve the ideal anti-aging effect.

[0005] In addition, exosomes, as a substance with potential biological activity, have attracted attention in the field of skin repair and anti-aging. However, how to rationally combine them with other ingredients to achieve synergistic enhancement while ensuring the stability and safety of the composition is still a technical problem that needs to be solved.

[0006] Therefore, a composition is proposed that can synergistically promote collagen synthesis, has good anti-inflammatory effects, is highly safe and has good stability, which is of great significance for meeting the market demand for anti-aging skin care. Summary of the Invention

[0007] In order to overcome the above-mentioned defects of the prior art, the present invention provides an exosome composition and a method and application thereof for promoting collagen synthesis to solve the problems raised in the above-mentioned background technology.

[0008] To achieve the above object, the present invention provides the following technical solution: an exosome composition comprising, by weight:

[0009] 15-30 parts of human umbilical cord mesenchymal stem cell exosomes, 8-15 parts of scutellaria baicalensis extract, 6 parts of acetyl hexapeptide-82, and 1-4 parts of zinc gluconate.

[0010] Exosomes derived from human umbilical cord mesenchymal stem cells carry various signaling molecules that regulate the function of skin fibroblasts. Baicalin in Scutellaria baicalensis extract has anti-inflammatory and antioxidant properties, reducing collagen damage caused by skin inflammation. Acetyl hexapeptide-8 inhibits neurotransmitter release, reduces wrinkle formation caused by muscle contraction, and promotes collagen synthesis. Zinc gluconate, as a zinc source, regulates the activity of multiple enzymes, promoting enzymatic reactions in collagen synthesis. The ingredients, formulated in a weight ratio of 15-30 parts human umbilical cord mesenchymal stem cell exosomes, 8-15 parts Scutellaria baicalensis extract, 2-6 parts acetyl hexapeptide-8, and 1-4 parts zinc gluconate, work synergistically to promote collagen synthesis and improve skin condition. This combination effectively promotes the synthesis of type I collagen by skin fibroblasts, enhances skin elasticity, reduces wrinkles, and exhibits excellent anti-aging effects.

[0011] Preferably, the method for preparing human umbilical cord mesenchymal stem cell exosomes comprises the following steps:

[0012] Human umbilical cord Wharton's jelly tissue was obtained and digested with 0.25% trypsin at 37°C for 30-45 minutes. The digestion was terminated by adding DMEM medium containing 10% fetal bovine serum and centrifuged at 1000 rpm for 10 minutes to obtain human umbilical cord mesenchymal stem cells.

[0013] Human umbilical cord mesenchymal stem cells were cultured in serum-free medium supplemented with 1% double antibody at 37°C and 5% CO2 for 48-72 hours, and the culture supernatant was collected;

[0014] The culture supernatant was first centrifuged at 3000g for 20 minutes at 4°C, the supernatant was filtered through a 0.22 μm filter membrane, and then ultracentrifuged at 100,000g for 70 minutes to collect the exosome precipitate, which was resuspended in PBS to obtain human umbilical cord mesenchymal stem cell exosomes.

[0015] Human umbilical cord Wharton's jelly tissue was digested with 0.25% trypsin at 37°C for 30-45 minutes to effectively isolate mesenchymal stem cells. Serum-free culture prevented interference of serum components on exosomes. Cell debris was removed by centrifugation at 3000g for 20 minutes, further purified by filtration through a 0.22μm filter membrane, and exosomes were collected by ultracentrifugation at 100,000g for 70 minutes to ensure the acquisition of high-purity and high-activity exosomes. The prepared human umbilical cord mesenchymal stem cell exosomes were of high purity and good activity, and could better play the role of promoting collagen synthesis.

[0016] Preferably, the baicalin content in the scutellaria baicalensis extract is not less than 95%, and the extract is extracted twice with 70% ethanol under reflux at 60°C, each time for 1-2 hours, the extracts are combined, concentrated under reduced pressure until there is no ethanol, and freeze-dried to obtain the extract.

[0017] Taking advantage of the fact that baicalin is easily soluble in 70% ethanol, reflux extraction is performed twice at 60°C, each time for 1-2 hours, to fully extract baicalin; reduced pressure concentration is performed to remove ethanol, and freeze-drying is performed to maintain the activity of the extract. The baicalin content in the prepared Scutellaria baicalensis extract is not less than 95%, has strong anti-inflammatory and antioxidant activity, and can effectively assist in promoting collagen synthesis.

[0018] Preferably, the acetyl hexapeptide-8 has a molecular weight of 624.7 Da, is prepared by solid phase synthesis, and has an amino acid sequence of Ac-Glu-Glu-Met-Ala-Arg-Arg-NH2.

[0019] The amino acid sequence of acetyl hexapeptide-8, Ac-Glu-Glu-Met-Ala-Arg-Arg-NH2, enables it to act on the neuromuscular junction, inhibit the release of acetylcholine, reduce muscle contraction, and at the same time promote the synthesis of collagen by fibroblasts through signal transduction, which can reduce the formation of dynamic wrinkles and promote collagen synthesis to make the skin firmer.

[0020] Preferably, the purity of the zinc gluconate is not less than 99%, and the molecular formula is C 12 H 22 O 14 Zn, zinc content ≥14.5%.

[0021] Zinc gluconate is a good source of zinc. Zinc is a coenzyme of many enzymes, such as collagenase. It participates in the synthesis and degradation regulation of collagen, promotes the normal synthesis of collagen, provides necessary trace elements for collagen synthesis, enhances the activity of related enzymes, and promotes collagen synthesis.

[0022] Preferably, the invention further comprises a moisturizing agent and a thickening agent, wherein the moisturizing agent is a mixture of glycerin and propylene glycol in a weight ratio of 2:1, and the addition amount is 5-10 parts by weight; the thickening agent is carbomer, and the addition amount is 0.3-0.8 parts by weight.

[0023] The moisturizer composed of glycerin and propylene glycol in a 2:1 ratio can absorb water, keep the skin moist, and provide a good environment for collagen synthesis; carbomer, as a thickener, can adjust the viscosity of the composition, making it more suitable for use in skin care products, and at the same time helps the composition adhere to and distribute on the skin surface, so that the composition has good moisturizing properties and a suitable dosage form, improving the feel and effect of use.

[0024] A method for preparing the exosome composition described above comprises the following steps:

[0025] The human umbilical cord mesenchymal stem cell exosomes were ultracentrifuged at a temperature of 4°C and a centrifugation time of 70 minutes;

[0026] Weigh the scutellaria baicalensis extract, acetyl hexapeptide-8, and zinc gluconate, add them to PBS at 37°C, and stir at 150 rpm for 25-30 minutes until completely dissolved;

[0027] Human umbilical cord mesenchymal stem cell exosomes were added to the above solution, mixed evenly, and then a moisturizer and a thickener were added. The pH was adjusted to 5.0-6.5, and sterilized using a 0.22 μm filter membrane to obtain an exosome composition.

[0028] The components are mixed in a specific order, and the dissolution temperature and stirring speed are controlled to ensure that the components are fully dissolved and mixed; the pH is adjusted to 5.0-6.5 to provide a suitable acidity and alkalinity environment for the composition to ensure its stability and activity; 0.22μm filter membrane sterilization ensures the safety of the composition. The prepared exosome composition is uniform and stable, and the active ingredients are well maintained, making it easy to use in skin care products.

[0029] A use of the exosome composition described above in promoting the synthesis of type I collagen by skin fibroblasts.

[0030] Preferably, the exosome composition is used to prepare anti-aging skin care products, which are in the form of essence, mask or gel, and the application amount is 2-3 mg / cm 2 .

[0031] Preferably, the preparation method of the essence is: mixing the exosome composition prepared according to claim 7 with Rosa damascena flower water in a weight ratio of 4:1, adding 0.8-1.2 parts by weight of 1,2-hexanediol and 0.15-0.25 parts by weight of ethylhexylglycerin, stirring evenly and then filling.

[0032] The exosome composition is applied to anti-aging skin care products. After absorption through the skin, the various components work synergistically to promote collagen synthesis and improve skin elasticity and texture. Different dosage forms can meet different usage needs, and a specific application amount ensures the effect of the active ingredients. A variety of anti-aging skin care products can be prepared, which are easy to use and can effectively promote collagen synthesis to achieve the purpose of anti-aging.

[0033] The exosome composition provided by the present invention and the method and application thereof for promoting collagen synthesis have the following beneficial effects:

[0034] The synergistic effect of human umbilical cord mesenchymal stem cell exosomes, Scutellaria baicalensis extract, acetyl hexapeptide-8 and zinc gluconate can significantly increase the expression level of type I collagen-related genes in skin fibroblasts, effectively promote the synthesis of type I collagen, enhance skin elasticity, reduce wrinkles, and have a significant anti-aging effect.

[0035] The composition can increase the expression level of the anti-inflammatory gene IL-10 and reduce the expression levels of the pro-inflammatory genes TNF-α and iNOS, effectively alleviate skin inflammatory reactions, reduce the damage of inflammation to the skin, and create a good environment for collagen synthesis.

[0036] Within the test concentration range (25-400 μg / mL), the composition has little effect on the survival rate of human skin fibroblasts, and the cell survival rate is above 92%, indicating that it is highly safe and suitable for skin care.

[0037] The preparation methods of exosomes and their components are scientific and can ensure the purity and activity of the active ingredients; the preparation process of the composition can ensure that the components are fully mixed, have good stability, and are easy to use. DETAILED DESCRIPTION

[0038] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0039] Example 1

[0040] Preparation of human umbilical cord mesenchymal stem cell exosomes: Human umbilical cord Wharton's jelly tissue was digested with 0.25% trypsin at 37°C for 30 minutes, and digestion was terminated by adding DMEM medium containing 10% fetal bovine serum. The cells were centrifuged at 1000 rpm for 10 minutes to obtain human umbilical cord mesenchymal stem cells. The cells were cultured in serum-free medium supplemented with 1% double-antibody at 37°C and 5% CO2 for 48 hours, and the culture supernatant was collected. The culture supernatant was first centrifuged at 3000g for 20 minutes at 4°C, the supernatant was filtered through a 0.22μm filter membrane, and then ultracentrifuged at 100,000g for 70 minutes. The exosome precipitate was collected and resuspended in PBS to obtain 20 human umbilical cord mesenchymal stem cell exosomes.

[0041] Preparation of Scutellaria baicalensis extract: Scutellaria baicalensis was extracted twice with 70% ethanol under reflux at 60°C for 1 hour each time. The extracts were combined, concentrated under reduced pressure until there was no ethanol, and freeze-dried to obtain 10 parts of Scutellaria baicalensis extract, in which the baicalin content was 95%.

[0042] Weigh 4 parts of acetyl hexapeptide-8 and 2 parts of zinc gluconate, add them to PBS at 37°C, and stir at 150 rpm for 25 minutes until completely dissolved; add the above-mentioned human umbilical cord mesenchymal stem cell exosomes, mix well, add 7 parts of a moisturizer composed of glycerol and propylene glycol in a ratio of 2:1 and 0.5 parts of a thickener carbomer, adjust the pH to 5.5, and sterilize with a 0.22 μm filter membrane to obtain an exosome composition.

[0043] Example 2

[0044] Preparation of human umbilical cord mesenchymal stem cell exosomes: Human umbilical cord Wharton's jelly tissue was digested with 0.25% trypsin at 37°C for 45 minutes, and digestion was terminated by adding DMEM medium containing 10% fetal bovine serum. The cells were centrifuged at 1000 rpm for 10 minutes to obtain human umbilical cord mesenchymal stem cells. The cells were cultured in serum-free medium supplemented with 1% double-antibody at 37°C and 5% CO2 for 72 hours, and the culture supernatant was collected. The culture supernatant was first centrifuged at 3000g for 20 minutes at 4°C, the supernatant was filtered through a 0.22μm filter membrane, and then ultracentrifuged at 100,000g for 70 minutes. The exosome precipitate was collected and resuspended in PBS to obtain 25 human umbilical cord mesenchymal stem cell exosomes.

[0045] Preparation of Scutellaria baicalensis extract: Scutellaria baicalensis was extracted twice with 70% ethanol under reflux at 60°C for 2 hours each time. The extracts were combined, concentrated under reduced pressure until there was no ethanol, and freeze-dried to obtain 12 parts of Scutellaria baicalensis extract, in which the baicalin content was 96%.

[0046] Weigh 5 parts of acetyl hexapeptide-8 and 3 parts of zinc gluconate, add them to PBS at 37°C, and stir at 150 rpm for 30 minutes until completely dissolved; add the above-mentioned human umbilical cord mesenchymal stem cell exosomes, mix well, add 8 parts of a moisturizer composed of glycerol and propylene glycol in a ratio of 2:1 and 0.6 parts of a thickener carbomer, adjust the pH to 6.0, and sterilize with a 0.22 μm filter membrane to obtain an exosome composition.

[0047] Example 3

[0048] Preparation of human umbilical cord mesenchymal stem cell exosomes: Human umbilical cord Wharton's jelly tissue was digested with 0.25% trypsin at 37°C for 35 minutes, and digestion was terminated by adding DMEM medium containing 10% fetal bovine serum. The cells were centrifuged at 1000 rpm for 10 minutes to obtain human umbilical cord mesenchymal stem cells. The cells were cultured in serum-free medium supplemented with 1% double-antibody at 37°C and 5% CO2 for 60 hours, and the culture supernatant was collected. The culture supernatant was first centrifuged at 3000g for 20 minutes at 4°C, the supernatant was filtered through a 0.22μm filter membrane, and then ultracentrifuged at 100,000g for 70 minutes. The exosome precipitate was collected and resuspended in PBS to obtain 18 human umbilical cord mesenchymal stem cell exosomes.

[0049] Preparation of Scutellaria baicalensis extract: Scutellaria baicalensis was extracted twice with 70% ethanol under reflux at 60°C, each time for 1.5 hours. The extracts were combined, concentrated under reduced pressure until there was no ethanol, and freeze-dried to obtain 9 parts of Scutellaria baicalensis extract, in which the baicalin content was 95.5%.

[0050] Weigh 3 parts of acetyl hexapeptide-8 and 1.5 parts of zinc gluconate, add them to PBS at 37°C, and stir at 150 rpm for 28 minutes until completely dissolved; add the above-mentioned human umbilical cord mesenchymal stem cell exosomes, mix evenly, add 6 parts of a moisturizer composed of glycerol and propylene glycol in a ratio of 2:1 and 0.4 parts of a thickener carbomer, adjust the pH to 5.8, and sterilize with a 0.22 μm filter membrane to obtain an exosome composition.

[0051] Comparative Example 1

[0052] Preparation of the composition: excluding human umbilical cord mesenchymal stem cell exosomes, the other components and preparation method are the same as in Example 1, i.e., 10 parts of Scutellaria baicalensis extract, 4 parts of acetyl hexapeptide-8, 2 parts of zinc gluconate, 7 parts of moisturizer, and 0.5 parts of carbomer as a thickener, and the composition is prepared in the same way.

[0053] Comparative Example 2

[0054] Preparation of the composition: excluding the Scutellaria baicalensis extract, the other components and preparation method are the same as in Example 1, namely, 20 parts of human umbilical cord mesenchymal stem cell exosomes, 4 parts of acetyl hexapeptide-8, 2 parts of zinc gluconate, 7 parts of moisturizer, and 0.5 parts of carbomer as a thickener, and the composition is prepared in the same way.

[0055] 3. Test Method

[0056] 1. Skin fibroblast type I collagen synthesis promotion test

[0057] Test principle: The ability of the composition to promote collagen synthesis is evaluated by detecting the expression levels of type I collagen-related genes (COL1A1, COL1A2) in skin fibroblasts.

[0058] Test method:

[0059] Human skin fibroblasts in logarithmic growth phase were collected and 1×10 4 The cells were seeded at a density of 1000 cells / well in 6-well plates and cultured for 24 hours.

[0060] The original culture medium was discarded, and serum-free culture medium containing the composition prepared in Example 1-3 and Comparative Example 1-2 was added respectively at a concentration of 100 μg / mL. Three replicate wells were set up for each group, and a blank control group (only serum-free culture medium) was set up.

[0061] After culturing for 48 hours, the culture medium was discarded, the cells were washed twice with PBS, and TRIzol reagent was added to extract total RNA.

[0062] RNA was reverse transcribed into cDNA using a reverse transcription kit, and then real-time fluorescence quantitative PCR was performed to detect the expression levels of COL1A1 and COL1A2 genes. GAPDH was used as the internal reference gene, and the relative expression levels were calculated using the 2^(-ΔΔCt) method. The test results are shown in Table 1 below.

[0063] Table 1: Test results of promoting type I collagen synthesis in skin fibroblasts

[0064] Group Relative expression of COL1A1 Relative expression of COL1A2 Blank control group 1.00±0.05 1.00±0.06 Example 1 3.25±0.12 2.89±0.10 Example 2 3.56±0.15 3.12±0.13 Example 3 3.18±0.11 2.78±0.09 Comparative Example 1 1.56±0.08 1.42±0.07 Comparative Example 2 2.15±0.09 1.98±0.08

[0065] 2. Anti-inflammatory activity test (taking RAW264.7 cells as an example)

[0066] Test principle: The anti-inflammatory activity of the composition is evaluated by detecting the expression levels of inflammation-related genes (IL-10, TNF-α, iNOS).

[0067] Test method:

[0068] RAW264.7 cells in the logarithmic growth phase were taken and 2×10 5 The cells were seeded at a density of 1000 cells / well in 6-well plates and cultured for 24 hours.

[0069] The original culture medium was discarded, and a culture medium containing LPS (1 μg / mL) was added for stimulation for 2 hours. Then, culture medium containing different compositions (Examples 1-3 and Comparative Examples 1-2, all at a concentration of 100 μg / mL) was added. Three replicate wells were set up in each group, and an LPS model group and a blank control group were also set up.

[0070] After culturing for 24 hours, the culture medium was discarded, the cells were washed twice with PBS, and TRIzol reagent was added to extract total RNA.

[0071] RNA was reverse transcribed into cDNA using a reverse transcription kit, and then real-time fluorescence quantitative PCR was performed to detect the expression levels of IL-10, TNF-α, and iNOS genes. GAPDH was used as the internal reference gene, and the relative expression levels were calculated using the 2^(-ΔΔCt) method. The test results are shown in Table 2 below.

[0072] Table 2: Anti-inflammatory activity test results

[0073]

[0074] 3. Cytotoxicity test (MTT method)

[0075] Test principle: Evaluate the toxicity of the composition to cells by detecting cell survival rate.

[0076] Test method:

[0077] Human skin fibroblasts in logarithmic growth phase were collected and cultured at 5×10 3 The cells were seeded at a density of 1000 cells / well in a 96-well plate and cultured for 24 hours.

[0078] The original culture medium was discarded, and serum-free culture medium containing different concentrations of the composition (Example 1, concentrations of 25, 50, 100, 200, and 400 μg / mL) was added respectively. Five replicate wells were set up for each group, and a blank control group was also set up.

[0079] After culturing for 48 hours, 20 μL of MTT solution (5 mg / mL) was added to each well and the culture was continued for another 4 hours.

[0080] The culture medium was discarded, 150 μL DMSO was added to each well, and the cells were shaken for 10 minutes to fully dissolve the crystals.

[0081] The absorbance value was measured at a wavelength of 570 nm using an enzyme marker, and the cell viability was calculated. The test results are shown in Table 3 below.

[0082] Table 3: Cytotoxicity test results

[0083] Composition concentration (μg / mL) Cell survival rate (%) 0 (blank control) 100.00±2.00 25 98.50±1.50 50 97.80±1.20 100 96.50±1.30 200 95.20±1.00 400 92.80±1.20

[0084] In summary, it can be seen that the relative expression levels of COL1A1 and COL1A2 in Examples 1-3 were significantly higher than those in the blank control group and Comparative Examples 1-2, indicating that the exosome composition can effectively promote the synthesis of type I collagen by skin fibroblasts, and the synergistic effect of each component is obvious.

[0085] The promotion effect of Example 2 is the best, which may be due to the more optimized ratio of the components.

[0086] Comparative Example 1 does not contain human umbilical cord mesenchymal stem cell exosomes, and its collagen synthesis promoting effect is significantly reduced, indicating that human umbilical cord mesenchymal stem cell exosomes are the key active ingredient of the composition; Comparative Example 2 does not contain Scutellaria baicalensis extract, and its effect is also reduced, indicating that Scutellaria baicalensis extract plays a role in assisting in promoting collagen synthesis in the composition.

[0087] Examples 1-3 can significantly increase the expression level of IL-10 and reduce the expression levels of TNF-α and iNOS, indicating that the composition has good anti-inflammatory activity, can reduce the damage of inflammation to the skin, and provide a good environment for collagen synthesis.

[0088] The anti-inflammatory effect of Example 2 is relatively good, which further illustrates that a reasonable ratio of the components is helpful to enhance the anti-inflammatory activity of the composition.

[0089] The anti-inflammatory effects of Comparative Example 1 and Comparative Example 2 were not as good as those of Example 1, which once again verified the importance of the synergistic effect of each component.

[0090] Within the test concentration range (25-400 μg / mL), the composition has little effect on the survival rate of human skin fibroblasts, and the cell survival rate is above 92%, indicating that the composition has good safety.

[0091] In summary, the exosome composition of the present invention can effectively promote the synthesis of type I collagen by skin fibroblasts through the synergistic effect of various components, has good anti-aging and anti-inflammatory effects, and is highly safe, and can be widely used in anti-aging skin care products.

[0092] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An exosome composition, characterized in that In parts by weight: 15-30 parts of human umbilical cord mesenchymal stem cell exosomes, 8-15 parts of scutellaria baicalensis extract, 6 parts of acetyl hexapeptide-82, and 1-4 parts of zinc gluconate.

2. The exosome composition according to claim 1, characterized in that The method for preparing human umbilical cord mesenchymal stem cell exosomes comprises the following steps: Human umbilical cord Wharton's jelly tissue was obtained and digested with 0.25% trypsin at 37°C for 30-45 minutes. The digestion was terminated by adding DMEM medium containing 10% fetal bovine serum and centrifuged at 1000 rpm for 10 minutes to obtain human umbilical cord mesenchymal stem cells. Human umbilical cord mesenchymal stem cells were cultured in serum-free medium supplemented with 1% double antibody at 37°C and 5% CO2 for 48-72 hours, and the culture supernatant was collected; The culture supernatant was first centrifuged at 3000g for 20 minutes at 4°C, the supernatant was filtered through a 0.22 μm filter membrane, and then ultracentrifuged at 100,000g for 70 minutes to collect the exosome precipitate, which was resuspended in PBS to obtain human umbilical cord mesenchymal stem cell exosomes.

3. The exosome composition according to claim 1, characterized in that The content of baicalin in the scutellaria baicalensis extract is not less than 95%. The extract is extracted twice with 70% ethanol under reflux at 60°C for 1-2 hours each time, and the extracts are combined, concentrated under reduced pressure until there is no ethanol, and freeze-dried to obtain the extract.

4. The exosome composition according to claim 1, characterized in that The acetyl hexapeptide-8 has a molecular weight of 624.7 Da, is prepared by solid-phase synthesis, and has an amino acid sequence of Ac-Glu-Glu-Met-Ala-Arg-Arg-NH2.

5. The exosome composition according to claim 1, characterized in that The molecular formula of the zinc gluconate is C 12 H 22 O 14 Zn,.

6. The exosome composition according to claim 1, characterized in that The invention also includes a moisturizing agent and a thickening agent. The moisturizing agent is a mixture of glycerin and propylene glycol in a weight ratio of 2:1, and the addition amount is 5-10 parts by weight; the thickening agent is carbomer, and the addition amount is 0.3-0.8 parts by weight.

7. A method for preparing the exosome composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: The human umbilical cord mesenchymal stem cell exosomes were ultracentrifuged at a temperature of 4°C and a centrifugation time of 70 minutes; Weigh the scutellaria baicalensis extract, acetyl hexapeptide-8, and zinc gluconate, add them to PBS at 37°C, and stir at 150 rpm for 25-30 minutes until completely dissolved; Human umbilical cord mesenchymal stem cell exosomes were added to the above solution, mixed evenly, and then a moisturizer and a thickener were added. The pH was adjusted to 5.0-6.5, and sterilized using a 0.22 μm filter membrane to obtain an exosome composition.

8. Use of the exosome composition according to any one of claims 1 to 6 in promoting the synthesis of type I collagen by skin fibroblasts.

9. The use according to claim 8, characterized in that The exosome composition is used to prepare anti-aging skin care products, which are in the form of essence, mask or gel, and the application amount is 2-3 mg / cm 2 .

10. The use according to claim 9, characterized in that The preparation method of the essence is: mixing the exosome composition prepared according to claim 7 with Rosa damascena flower water in a weight ratio of 4:1, adding 0.8-1.2 parts by weight of 1,2-hexanediol and 0.15-0.25 parts by weight of ethylhexylglycerin, stirring evenly and then filling.

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