An anti-wrinkle and whitening composition and its preparation method

Abstract

This invention relates to an anti-wrinkle and whitening composition and its preparation method, belonging to the field of cosmetic technology. The composition includes the following raw materials: complex peptides, hydroxypropyl tetrahydropyranotriol, plant extracts, plant oils, emulsifiers, preservatives, and solvents. This application selects acetyl hexapeptide-7 and tripeptide-10 citrulline as complex peptides, combined with *Sedum morganianum* bulb extract and hydroxypropyl tetrahydropyranotriol as the main anti-wrinkle components. This combination works from different dimensions—reducing dynamic wrinkles, increasing collagen content, and increasing extracellular matrix content—thereby assisting in enhancing collagen deposition. The synergistic effect results in a finished composition with excellent anti-wrinkle effects. Furthermore, this application also uses *Patrinia scabiosifolia* extract, *Lepidium apetalum* root extract, grape seed oil, and *Phyllostachys edulis* calyx extract in combination to synergistically exert outstanding whitening effects from different dimensions—inhibiting melanocyte activity, reducing melanin production, and reducing pigmentation.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle and whitening composition and its preparation method. Background Technology

[0002] In the cosmetics industry, anti-wrinkle and whitening are two core demands that consumers have long focused on. With rising living standards and changing aesthetic standards, consumer demand for cosmetics that combine both anti-wrinkle and whitening effects is increasing. However, current cosmetic compositions on the market still have many shortcomings in terms of anti-wrinkle and whitening effects. Most existing anti-wrinkle compositions often rely on a single active ingredient such as retinol or peptides, which can easily limit the amount added due to skin tolerance issues, resulting in slow and short-lasting wrinkle improvement. As for whitening, many compositions only focus on inhibiting melanin production, lacking effective interventions for existing melanin deposits, thus significantly reducing the whitening effect.

[0003] Therefore, by rationally combining various active ingredients, a cosmetic composition that can effectively reduce wrinkles and significantly whiten skin can be developed, which can effectively overcome the limitations of existing compositions in terms of anti-wrinkle and whitening effects, and provide consumers with a better choice of cosmetics. Summary of the Invention

[0004] The purpose of this invention is to provide an anti-wrinkle and whitening composition and its preparation method, which solves the problem of poor anti-wrinkle and whitening effects in existing compositions used to prepare cosmetic products.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] The first aspect of this invention provides an anti-wrinkle and whitening composition comprising the following raw materials in parts by weight:

[0007] 3-5.5 parts by weight of complex peptide, 2-5 parts by weight of hydroxypropyl tetrahydropyrantriol, 8.2-12.3 parts by weight of plant extract, 6-10 parts by weight of plant oil, 5-10 parts by weight of emulsifier, 0.1-0.5 parts by weight of preservative, and 61-70 parts by weight of solvent.

[0008] Hydroxypropyltetrahydropyrantriol has the ability to increase the content of extracellular matrix and can also promote the synthesis of elastin and collagen.

[0009] As a preferred embodiment of the present invention, the complex peptide includes at least one of acetyl hexapeptide-1, acetyl hexapeptide-7, acetyl hexapeptide-8, and tripeptide-10 citrulline.

[0010] Preferably, the complex peptide comprises acetyl hexapeptide-7 and tripeptide-10 citrulline;

[0011] Acetyl hexapeptide-7 has the ability to stimulate the proliferation and migration of keratinocytes, and can also induce collagen synthesis, which can effectively reduce the depth of dynamic wrinkles;

[0012] Tripeptide-10 citrulline can further stabilize the extracellular matrix content increased by hydroxypropyl tetrahydropyranotriol and improve wrinkles, thus playing a certain assisting role.

[0013] Further, the complex peptide comprises 2-4 parts by weight of acetyl hexapeptide-7 and 1-1.5 parts by weight of tripeptide-10 citrulline.

[0014] As a preferred embodiment of the present invention, the plant extracts include *Sedum aizoon* bulb extract, *Patrinia scabiosifolia* extract, *Lepidium apetalum* root extract, and *Phyllostachys edulis* calyx extract.

[0015] The bulb extract of Leucojuum aestivum has the ability to activate the TGF-β / Smad signaling pathway, thereby increasing collagen content;

[0016] The extract of Patrinia scabiosaefolia contains components such as valerin, isovalerin, hyoscyamine, oleanolic acid, and quercetin, which have inhibitory effects on the activity of B-16 melanocytes and can effectively reduce melanin content.

[0017] Lepidium meyenii root extract contains active ingredients such as fatty acids, phytosterols and maca alkaloids, which have a certain inhibitory effect on tyrosinase and can block the melanin synthesis pathway.

[0018] Physalis alkekengi calyx extract is rich in vitamin C, steroidal compounds, sugar acids, sterols and other ingredients, which can reduce pigmentation, brighten skin tone and make the skin whiter and more translucent.

[0019] Furthermore, the plant extracts include 4-7 parts by weight of *Sedum morganianum* bulb extract, 1-1.5 parts by weight of *Patrinia scabra* extract, 1.5-2 parts by weight of *Lepidium apetalum* root extract, and 1-2 parts by weight of *Phyllanthus urinaria* calyx extract.

[0020] As a preferred embodiment of the present invention, the vegetable oil includes any one of grape seed oil, carrot seed oil, and lemon peel oil.

[0021] Preferably, the vegetable oil includes grape seed oil;

[0022] Grape (VITIS VINIFERA) seed oil contains polyphenols, proanthocyanidins, fats, pantothenic acid, and vitamins, which can accelerate metabolism and prevent melanin deposition.

[0023] As a preferred embodiment of the present invention, the emulsifier includes at least one of cetyl alcohol, PEG-10 glyceryl stearate, and PEG-75 stearate, the preservative includes p-hydroxyacetophenone, and the solvent includes deionized water.

[0024] The second aspect of the present invention provides a method for preparing an anti-wrinkle and whitening composition, comprising the following steps:

[0025] S1. Mix the complex peptide, hydroxypropyltetrahydropyranotriol and solvent under controlled temperature to obtain mixture A;

[0026] S2. Mix the vegetable oil, emulsifier and preservative under controlled temperature to obtain mixture B;

[0027] S3. Add mixture A to mixture B, control the temperature and homogenize until uniform, then cool down, add plant extract, mix and homogenize until uniform, let stand, and the finished product is obtained.

[0028] As a preferred embodiment of the present invention, the temperature controlled in step S1 is 30-35℃.

[0029] As a preferred embodiment of the present invention, the temperature controlled in step S2 is 35-40℃.

[0030] As a preferred technical solution of the present invention, the temperature for temperature control and homogenization in step S3 is 80-85℃, the temperature for cooling is 40-45℃, and the standing is settling to room temperature.

[0031] The third aspect of this invention provides the application of an anti-wrinkle and whitening composition in the preparation of cosmetics, wherein the anti-wrinkle and whitening composition is added at 3-5% of the total mass of the cosmetic.

[0032] As a preferred embodiment of the present invention, the cosmetics include, but are not limited to, toners, serums, face creams, and moisturizers.

[0033] The beneficial effects of this invention are:

[0034] (1) This application uses two peptides, snowdrop bulb extract and hydroxypropyl tetrahydropyranotriol as the main anti-wrinkle components. Acetyl hexapeptide-7 blocks the transmission of nerve signals to muscles and rapidly reduces dynamic wrinkles; snowdrop bulb extract increases the content of collagen; at the same time, hydroxypropyl tetrahydropyranotriol is combined with tripeptide-10 citrulline to increase the content of extracellular matrix and help enhance the deposition of collagen. Therefore, the finished product of the composition obtained by this method has excellent anti-wrinkle effect.

[0035] (2) This application uses Patrinia scabiosifolia extract to inhibit B-16 melanocyte activity. At the same time, it combines Lepidium apetalum root extract and grape seed oil. The fatty acid chain in Lepidium apetalum root extract and the phenolic substances in grape seed oil can synergistically regulate the NF-κB and Nrf2 / ARE pathways, enhance the antioxidant capacity of cells, reduce UV-induced melanin production and prevent melanin deposition to a certain extent. On this basis, Prunus armeniaca calyx extract is used to reduce pigmentation and brighten skin tone. Therefore, the finished product of the composition can synergistically exert outstanding whitening effects from different dimensions such as inhibiting melanocyte activity, reducing melanin production and reducing pigmentation. Detailed Implementation

[0036] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0037] All raw materials used in this application are commercially available products. Specifically, the extract of *Sedum aizoon* bulb (product number: xcsw20210331) was purchased from Ningxia Xiangcao Biotechnology Co., Ltd.; the extract of *Patrinia scabiosifolia* was purchased from Ningxia Xiangcao Biotechnology Co., Ltd.; the extract of *Lepidium apetalum* root was purchased from Shanghai Boshuo Industrial Co., Ltd.; the extract of *Phyllanthus urinaria* calyx was purchased from Fufeng Sinote Biotechnology Co., Ltd.; and the grape seed oil was purchased from Nanjing Bermuda Biotechnology Co., Ltd. Further details will not be repeated hereafter.

[0038] Example 1

[0039] An anti-wrinkle and whitening composition comprising the following raw materials in parts by weight:

[0040] 4.2 parts by weight of complex peptide, 2 parts by weight of hydroxypropyl tetrahydropyrantriol, 8.2 parts by weight of plant extract, 8 parts by weight of plant oil, 10 parts by weight of emulsifier, 0.1 parts by weight of preservative, and 67.5 parts by weight of solvent.

[0041] The complex peptide comprises 3 parts by weight of acetyl hexapeptide-7 and 1.2 parts by weight of tripeptide-10 citrulline;

[0042] The plant extracts include 4 parts by weight of *Sedum morganianum* bulb extract, 1.2 parts by weight of *Patrinia scabra* extract, 1.5 parts by weight of *Lepidium apetalum* root extract, and 1.5 parts by weight of *Phyllanthus urinaria* calyx extract.

[0043] The vegetable oil includes grape seed oil;

[0044] The emulsifier includes cetyl alcohol;

[0045] The preservative includes p-hydroxyacetophenone;

[0046] The solvent includes deionized water;

[0047] The preparation method of the anti-wrinkle and whitening composition includes the following steps:

[0048] S1. Mix the complex peptide, hydroxypropyl tetrahydropyranotriol and solvent at a controlled temperature of 32.5℃ to obtain mixture A;

[0049] S2. Mix the vegetable oil, emulsifier and preservative at a controlled temperature of 40°C to obtain mixture B;

[0050] S3. Add mixture A to mixture B, control the temperature to 80℃ and homogenize. Then, cool the temperature to 45℃, add the plant extract, mix and homogenize. Let stand at room temperature to obtain the finished composition.

[0051] Example 2

[0052] An anti-wrinkle and whitening composition comprising the following raw materials in parts by weight:

[0053] 3 parts by weight of complex peptide, 5 parts by weight of hydroxypropyl tetrahydropyrantriol, 12.3 parts by weight of plant extract, 10 parts by weight of plant oil, 7.5 parts by weight of emulsifier, 0.3 parts by weight of preservative, and 61.9 parts by weight of solvent.

[0054] The complex peptide comprises 2 parts by weight of acetyl hexapeptide-7 and 1 part by weight of tripeptide-10 citrulline.

[0055] The plant extracts include 7 parts by weight of *Sedum morganianum* bulb extract, 1.5 parts by weight of *Patrinia scabra* extract, 1.8 parts by weight of *Lepidium apetalum* root extract, and 2 parts by weight of *Phyllanthus urinaria* calyx extract.

[0056] The vegetable oil includes grape seed oil;

[0057] The emulsifier includes PEG-75;

[0058] The preservative includes p-hydroxyacetophenone;

[0059] The solvent includes deionized water;

[0060] The preparation method of the anti-wrinkle and whitening composition includes the following steps:

[0061] S1. Mix the complex peptide, hydroxypropyl tetrahydropyranotriol and solvent at a controlled temperature of 30°C to obtain mixture A;

[0062] S2. Mix the vegetable oil, emulsifier and preservative at a controlled temperature of 35°C to obtain mixture B;

[0063] S3. Add mixture A to mixture B, control the temperature to 82.5℃ and homogenize until uniform. Then, cool the temperature to 42.5℃, add the plant extract, mix and homogenize until uniform. Let stand to room temperature to obtain the finished composition.

[0064] Example 3

[0065] An anti-wrinkle and whitening composition comprising the following raw materials in parts by weight:

[0066] 5.5 parts by weight of complex peptide, 3.5 parts by weight of hydroxypropyl tetrahydropyrantriol, 9.5 parts by weight of plant extract, 6 parts by weight of plant oil, 5 parts by weight of emulsifier, 0.5 parts by weight of preservative, and 70 parts by weight of solvent.

[0067] The complex peptide comprises 4 parts by weight of acetyl hexapeptide-7 and 1.5 parts by weight of tripeptide-10 citrulline.

[0068] The plant extracts include 5.5 parts by weight of *Sedum morganianum* bulb extract, 1 part by weight of *Patrinia scabra* extract, 2 parts by weight of *Lepidium apetalum* root extract, and 1 part by weight of *Phyllanthus urinaria* calyx extract.

[0069] The vegetable oil includes grape seed oil;

[0070] The emulsifier includes PEG-10 glyceryl stearate;

[0071] The preservative includes p-hydroxyacetophenone;

[0072] The solvent includes deionized water;

[0073] The preparation method of the anti-wrinkle and whitening composition includes the following steps:

[0074] S1. Mix the complex peptide, hydroxypropyl tetrahydropyranotriol and solvent at a controlled temperature of 35°C to obtain mixture A;

[0075] S2. Mix the vegetable oil, emulsifier, and preservative at a controlled temperature of 37.5℃ to obtain mixture B;

[0076] S3. Add mixture A to mixture B, control the temperature to 85℃ and homogenize. Then, cool the temperature to 40℃, add the plant extract, mix and homogenize. Let stand at room temperature to obtain the finished composition.

[0077] Comparative Example 1

[0078] Compared with Example 2, the difference is that Acetyl hexapeptide-7 was not added in Comparative Example 1, and the missing weight was made up with tripeptide-10 citrulline, hydroxypropyl tetrahydropyranotriol and snow lotus bulb extract in a mass ratio of 1:5:7. All other operation steps and parameters remained unchanged.

[0079] Comparative Example 2

[0080] Compared with Example 2, the difference is that tripeptide-10 citrulline was not added in Comparative Example 2, and the missing weight was made up with acetyl hexapeptide-7, hydroxypropyl tetrahydropyranotriol and snowdrop bulb extract in a mass ratio of 2:5:7. All other operation steps and parameters remained unchanged.

[0081] Comparative Example 3

[0082] Compared with Example 2, the difference is that hydroxypropyl tetrahydropyranotriol was not added in Comparative Example 3, and the missing weight was made up with acetyl hexapeptide-7, tripeptide-10 citrulline and snowdrop bulb extract in a mass ratio of 2:1:7. All other operation steps and parameters remained unchanged.

[0083] Comparative Example 4

[0084] Compared with Example 2, the difference is that Comparative Example 4 did not add the extract of *Sedum aizoon* bulb, and the missing weight was made up with acetyl hexapeptide-7, tripeptide-10 citrulline, and hydroxypropyl tetrahydropyranotriol in a mass ratio of 2:1:5. All other operating steps and parameters remained unchanged.

[0085] Comparative Example 5

[0086] Compared with Example 2, the difference is that Comparative Example 5 did not add Patrinia scabiosifolia extract, and the missing weight was made up with Lepidium apetalum root extract, grape seed oil and Prunus armeniaca calyx extract in a mass ratio of 0.9:5:1. All other operation steps and parameters remained unchanged.

[0087] Comparative Example 6

[0088] Compared with Example 2, the difference is that Comparative Example 6 does not add Lepidium apetalum root extract, and the missing weight is made up with Patrinia scabiosifolia extract, grape seed oil and Prunus armeniaca calyx extract in a mass ratio of 1.5:10:2. All other operation steps and parameters remain unchanged.

[0089] Comparative Example 7

[0090] Compared with Example 2, the difference is that grape seed oil was not added in Comparative Example 7, and the missing weight was made up with extracts of Patrinia scabiosifolia, Lepidium apetalum root extract and Pyrrosia lingua calyx extract in a mass ratio of 1.5:1.8:2. All other operation steps and parameters remained unchanged.

[0091] Comparative Example 8

[0092] Compared with Example 2, the difference is that Comparative Example 8 does not add calyx extract, and the missing weight is made up with extracts of Patrinia scabiosifolia, Lepidium apetalum root extract and grape seed oil in a mass ratio of 1.5:1.8:10. All other operating steps and parameters remain unchanged.

[0093] Test Example 1: Anti-wrinkle effect test

[0094] The compositions obtained in Examples 1-3 and Comparative Examples 1-4 were added to the same essence matrix at 3% of the total essence mass to prepare essence samples. Healthy female volunteers aged 30-50 years with similar degrees of facial wrinkles were randomly invited as subjects and randomly divided into 7 groups of 12 people each. Each group tried one sample. The sample was applied to the wrinkled area of ​​the subjects' cheeks. The method of application was as follows: the subjects took 1 mL of the corresponding essence for each group, applied it evenly to the wrinkled area of ​​their cheeks, and massaged it until fully absorbed. It was used twice a day, morning and evening, for 28 consecutive days. The Antera 3D skin analyzer was used to test the number of wrinkles and the degree of reduction in wrinkle area in the subjects' cheek wrinkled areas. The average value of each group was taken, and the results are shown in Table 1.

[0095] Table 1

[0096]

[0097] As shown in Table 1, the composition obtained in this application exhibits excellent anti-wrinkle effects. This is because the present application selects acetyl hexapeptide-7 and tripeptide-10 citrulline as complex peptides, which, together with *Sedum morganianum* bulb extract and hydroxypropyl tetrahydropyranotriol, serve as the main anti-wrinkle components. This synergistic effect addresses wrinkles from different dimensions, including reducing dynamic wrinkles, increasing collagen content, and increasing extracellular matrix content, thereby enhancing collagen deposition.

[0098] Test Example 2: Whitening Efficacy Test

[0099] Determination of tyrosinase inhibition rate: Using 10 mL test tubes, set up sample tubes (T2), sample background (T1), enzyme reaction tubes (C2), and solvent background (C1). For each sample and each test concentration, three parallel tubes should be set up for each sample tube (T2) and enzyme reaction tube (C2). The compositions prepared in Examples 1-3 and Comparative Examples 5-8 were used as sample solutions. Add the samples according to Table 2 and mix thoroughly. Incubate in a 37°C water bath for 10 min. Add 2 mL of levodopa solution to each tube sequentially, controlling the reaction time for each tube to 5 min. Immediately transfer the reaction solution from each tube to a cuvette and measure the absorbance at 475 nm. Calculate the tyrosinase inhibition rate of the composition using the following formula:

[0100] Tyrosinase inhibition rate (%) = 1 - [(AT2 -A T1 ) / A C2 -A C1 )];

[0101] Table 2 Sampling Requirements

[0102]

[0103] The results are shown in Table 3.

[0104] Table 3

[0105]

[0106] As can be clearly seen from Table 3, the finished product of the composition obtained in this application has outstanding whitening effects. This is because this application selects extracts of Patrinia scabiosifolia, Lepidium apetalum root extract, grape seed oil, and Prunus armeniaca calyx extract. These four ingredients can work synergistically to exert outstanding whitening effects by inhibiting melanocyte activity, reducing melanin production, and reducing pigmentation from different dimensions.

[0107] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0108] The above description is merely an example and illustration of the concept of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described or use similar methods to replace them, as long as they do not deviate from the concept of the invention or exceed the scope defined in the claims, they should all fall within the protection scope of the present invention.

Claims

1. An anti-wrinkle and whitening composition, characterized in that, Including the following parts by weight of raw materials: 3-5.5 parts by weight of complex peptide, 2-5 parts by weight of hydroxypropyl tetrahydropyrantriol, 8.2-12.3 parts by weight of plant extract, 6-10 parts by weight of plant oil, 5-10 parts by weight of emulsifier, 0.1-0.5 parts by weight of preservative, and 61-70 parts by weight of solvent. The complex peptide is 2-4 parts by weight of acetyl hexapeptide-7 and 1-1.5 parts by weight of tripeptide-10 citrulline; The plant extracts are 4-7 parts by weight of *Sedum morganianum* bulb extract, 1-1.5 parts by weight of *Patrinia scabiosifolia* extract, 1.5-2 parts by weight of *Lepidium apetalum* root extract, and 1-2 parts by weight of *Phyllanthus urinaria* calyx extract. The vegetable oil is grape seed oil.

2. The anti-wrinkle and whitening composition according to claim 1, characterized in that, The emulsifier includes at least one of cetyl alcohol, PEG-10 glyceryl stearate, and PEG-75 stearate, the preservative includes p-hydroxyacetophenone, and the solvent includes deionized water.

3. A method for preparing the anti-wrinkle and whitening composition according to any one of claims 1-2, characterized in that, Includes the following steps: S1. Mix the complex peptide, hydroxypropyltetrahydropyranotriol and solvent under controlled temperature to obtain mixture A; S2. Mix the vegetable oil, emulsifier and preservative under controlled temperature to obtain mixture B; S3. Add mixture A to mixture B, control the temperature and homogenize until uniform, then cool down, add plant extract, mix and homogenize until uniform, let stand, and the finished product is obtained.

4. The method for preparing the anti-wrinkle and whitening composition according to claim 3, characterized in that, The temperature controlled in step S1 is 30-35℃; the temperature controlled in step S2 is 35-40℃.

5. The method for preparing the anti-wrinkle and whitening composition according to claim 3, characterized in that, The temperature for temperature control and homogenization in step S3 is 80-85℃, the temperature for cooling is 40-45℃, and the standing time is to let it stand until it reaches room temperature.

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