Cold dispelling and dampness expelling massage medicated oil and preparation method thereof

Abstract

The application relates to the technical field of medicines, in particular to a cold dispelling and damp dispelling massage medicinal oil and a preparation method thereof. The cold dispelling and damp dispelling massage medicinal oil is prepared from the following raw materials in parts by weight: medicinal oil 40-80 parts, oil phase 17-19 parts, deionized water 35-45 parts and antioxidant 0.2-0.3 parts. The medicinal oil is prepared by extracting and compounding traditional Chinese medicine powder from sesame oil, and the traditional Chinese medicine powder comprises chebula, smilax, saussurea costus, storax, mace, cumin, asafetida, white mustard seed, safflower, fructus amomi roxburghii, amomum, clove, zanthoxylum and licorice. The cold dispelling and damp dispelling massage medicinal oil is prepared by homogenizing and emulsifying the medicinal oil, the oil phase, the deionized water and the antioxidant through gradient homogenization, and the cold dispelling and damp dispelling massage medicinal oil has the effects of dispelling cold and damp, dredging meridians, and is low in sensitivity, high in efficiency and high in safety.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical technology, and in particular to a massage oil for dispelling cold and dampness and its preparation method. Background Technology

[0002] With the promotion of the theory of external treatment in traditional Chinese medicine, massage oils based on Chinese herbal medicines have gradually become a commonly used preparation for unblocking meridians, relieving muscle soreness, joint stiffness and chronic strain due to their convenience, local targeting and natural ingredients.

[0003] Despite considerable research, current massage oils still face several key challenges: First, a trade-off between transdermal efficiency and safety: While chemical penetration enhancers, such as azone, can improve absorption, long-term use can damage the skin barrier or cause allergic reactions. Second, uneven penetration of compound ingredients: In traditional compound oils, the difference in polarity between lipid-soluble and water-soluble components leads to selective penetration during transdermal absorption, resulting in an imbalance in the proportion of active ingredients and affecting overall efficacy. Third, inactivation and irritation of active ingredients: Some volatile components are prone to oxidative degradation during preparation or storage; direct application of medicinal materials such as white mustard seed may cause skin burning and blisters. How to achieve efficient and balanced transdermal absorption of multiple components in compound oils without relying on chemically synthesized penetration enhancers, while simultaneously ensuring component stability and safety, remains a pressing technical challenge. Summary of the Invention

[0004] To address the core issues of low transdermal efficiency, component imbalance, and irritation in current cold-dispelling and dampness-removing massage oils, this invention provides a cold-dispelling and dampness-removing massage oil and its preparation method through optimization of a natural penetration-enhancing system, synergistic effects of multiple components in traditional Chinese medicine ("principal, assistant, adjuvant, and guide"), and innovation in preparation technology. This oil has the effects of dispelling cold and dampness, clearing meridians, and is low-allergenic, highly effective, and safe.

[0005] Firstly, this application provides a massage oil for dispelling cold and dampness, employing the following technical solution:

[0006] A massage oil for dispelling cold and dampness is made from the following raw materials in parts by weight: 40-80 parts of medicinal oil, 17-19 parts of oil phase, 35-45 parts of deionized water, and 0.2-0.3 parts of antioxidant.

[0007] A massage oil for dispelling cold and dampness is made from the following raw materials in parts by weight: 60 parts of medicinal oil, 18.6 parts of oil phase, 40 parts of deionized water, and 0.25 parts of antioxidant.

[0008] Preferably, the medicinal oil is a compound Chinese medicine powder and sesame oil in a mass ratio of 1:(8-10).

[0009] By adopting the above scheme, sesame oil has a high solubility for volatile oils, terpenes, alkaloids, fat-soluble vitamins and other effective components, which is superior to water extraction or alcohol extraction. At the same time, the oil phase can isolate oxygen and reduce oxidative degradation. With sesame oil as the matrix, there is no need to separate the solvent after extraction, which simplifies the process and ensures high safety. Sesame oil as the matrix can also more easily penetrate the skin lipid barrier, thereby improving the transdermal absorption efficiency of the cold-dispelling and dampness-removing massage oil.

[0010] Preferably, the compound Chinese medicine powder is prepared from the following raw materials in parts by weight: 20-35 parts of Terminalia chebula, 25-40 parts of Tinospora sinensis powder, 34-43 parts of Aucklandia lappa, 37-46 parts of Benzoin, 110-125 parts of Myristica fragrans, 54-63 parts of Fennel, 54-63 parts of Ferula assa-foetida, 36-42 parts of Sinapis alba, 7-16 parts of Carthamus tinctorius, 9-15 parts of Amomum tsao-ko, 7-16 parts of Amomum cardamomum, 9-15 parts of Clove, 27-36 parts of Zanthoxylum bungeanum, and 50-65 parts of Nardostachys jatamansi.

[0011] By adopting the above-mentioned treatment plan, with the core principles of warming yang and dispelling cold, eliminating dampness and unblocking meridians, and promoting qi and blood circulation, it conforms to the treatment principle of "treating cold with heat and dampness with dryness" in the Yellow Emperor's Inner Classic. Through the core theory of "principal, assistant, adjuvant, and guide" in the formulation, the principal herbs are: white mustard seed to warm the meridians and dispel cold, Sichuan pepper to dispel cold and relieve pain, and nutmeg to warm the middle and resolve dampness, forming a "dual-pronged approach" to directly target the root cause of cold and dampness; the assistant herbs are: *Caulis Clematis* to relax muscles and activate meridians, *Nardostachys chinensis* to promote qi and relieve pain, and *Benzoicum benzoin* to open the orifices and clear stagnation, fennel to dispel cold and promote qi and relieve pain, and costus root to relieve depression and pain, enhancing the warming and unblocking effect; the adjuvant herbs are: chebula to astringe and prevent excessive warming and drying, asafoetida to break up blood stasis and eliminate accumulation, and safflower to promote blood circulation and remove blood stasis, harmonizing the properties of the herbs and preventing warming and drying from damaging yin; and the guiding herbs are: clove to guide the herbs to the meridians, cardamom to invigorate the spleen and resolve dampness, and tsaoko to resolve turbidity, guiding the medicinal power to the surface and into the meridians. This formula is based on the principle of "warming and unblocking". Through the structure of "principal medicine to drive out cold and dampness, assistant medicine to penetrate the meridians, adjuvant medicine to stabilize, and guide medicine to the meridians", it has the effects of dispelling cold and dampness, unblocking the meridians, and is low in allergens, highly effective and safe.

[0012] Preferably, the compound Chinese medicine powder is prepared from the following raw materials in parts by weight: 25-30 parts of Terminalia chebula, 30-35 parts of Tinospora sinensis powder, 37-40 parts of Aucklandia lappa, 40-43 parts of Benzoin, 115-120 parts of Myristica fragrans, 57-60 parts of Fennel, 57-60 parts of Ferula assa-foetida, 38-40 parts of Sinapis alba, 10-13 parts of Carthamus tinctorius, 11-13 parts of Amomum tsao-ko, 10-13 parts of Amomum cardamomum, 11-13 parts of Clove, 30-33 parts of Zanthoxylum bungeanum, and 55-60 parts of Nardostachys jatamansi.

[0013] Preferably, the compound Chinese medicine powder is prepared from the following raw materials in parts by weight: 28 parts of Terminalia chebula, 34.8 parts of Tinospora sinensis powder, 38.6 parts of Aucklandia lappa, 42.6 parts of Benzoin, 119 parts of Myristica fragrans, 59 parts of Fennel, 57 parts of Ferula assa-foetida, 39.6 parts of Sinapis alba, 11.6 parts of Carthamus tinctorius, 12.5 parts of Amomum tsao-ko, 12.5 parts of Amomum villosum, 11.6 parts of Clove, 29 parts of Zanthoxylum bungeanum, and 56 parts of Nardostachys jatamansi.

[0014] Preferably, the powder of *Vitis thunbergii* is enzymatically hydrolyzed with cellulase, and the following technical solution is adopted: weigh *Vitis thunbergii* powder, sterilize it under high temperature and high pressure to eliminate interference from endogenous enzymes, add buffer solution and mix well, treat it with cellulase shaker, after the enzymatic hydrolysis is completed, immediately boil it in water bath for 10 minutes to inactivate the enzyme, centrifuge to collect the hydrolyzed *Vitis thunbergii* powder, and dry it.

[0015] By adopting the above scheme, cellulase hydrolyzes the cell wall of *Ipomoea quamoclit* in a short time, effectively destroying the cellulose structure and releasing small molecule triterpenoid active ingredients from the cells.

[0016] Preferably, the broadleaf vine powder is enzymatically hydrolyzed with cellulase, and the broadleaf vine powder, buffer solution and cellulase are in a mass ratio of 5:(18-22):1.

[0017] Preferably, the *Vitis thunbergii* powder is enzymatically hydrolyzed using cellulase, employing the following technical solution: Weigh the *Vitis thunbergii* powder, sterilize at 121℃ for 20 min to eliminate endogenous enzyme interference, add buffer solution and mix well, then treat with cellulase at 40-60℃ and 130-180 rpm on a shaker for 2-4 h to release small molecule triterpenes. Centrifuge at 3000 rpm for 5 min, collect the hydrolyzed *Vitis thunbergii* powder, and dry with hot air at 80-120℃ for 0.5-2 h.

[0018] Preferably, the buffer solution is a 0.05M citrate-disodium hydrogen phosphate buffer solution with a pH of 4.8 ± 0.1.

[0019] Preferably, the white mustard seeds are stir-fried at 120-150℃ for 5-8 minutes until they turn dark yellow.

[0020] The irritation of white mustard seeds mainly comes from their glucosinolate content. Under the action of myrosinase, glucosinolate decomposes to produce allyl isothiocyanate, which causes local burning sensation and erythema on the skin. In severe cases, it can cause persistent redness and swelling, papules or vesicles, urticaria, and systemic itching. By adopting the above method, the roasted white mustard seeds reduce adverse skin irritation. At the same time, roasting can kill enzymes and preserve glycosides, thereby reducing skin irritation.

[0021] Preferably, the compound Chinese medicine powder is obtained by pulverizing each Chinese medicine raw material, passing it through a 40-80 mesh sieve, weighing it according to the proportion, and mixing it with a three-dimensional mixer for 10-30 minutes.

[0022] Preferably, the oil phase is prepared from the following raw materials in parts by weight: 11-17 parts of sorbitol polyether-30 tetraoleate, 1-2.5 parts of cocamidopropyl betaine, 1-2.5 parts of tea saponin, and 0.5-1.5 parts of sodium lauroyl sarcosinate.

[0023] By adopting the above scheme, the emulsifier oil phase obtained by compounding sorbitol polyether-30 tetraoleate, cocamidopropyl betaine, tea saponin and sodium lauroyl sarcosinate has the advantages of improved dispersibility of fat-soluble components by the addition of sodium lauroyl sarcosinate, and the combination of tea saponin as a plant-derived surfactant with the functions of improving the stability of the emulsion system and antibacterial function, while reducing skin irritation. This formula reduces the amount of synthetic emulsifier used through natural-synthetic compounding, mild design and multi-component synergy, and achieves the effect of high-efficiency emulsification and low skin irritation.

[0024] Preferably, the oil phase is prepared from the following raw materials in parts by weight: 13-15 parts of sorbitol polyether-30 tetraoleate, 1.5-2 parts of cocamidopropyl betaine, 1.5-2 parts of tea saponin, and 0.8-1.2 parts of sodium lauroyl sarcosinate.

[0025] Preferably, the oil phase is prepared from the following raw materials in parts by weight: 14.6 parts of sorbitol polyether-30 tetraoleate, 1.6 parts of cocamidopropyl betaine, 1.5 parts of tea saponin, and 1 part of sodium lauroyl sarcosinate.

[0026] Preferably, the antioxidant is prepared from the following raw materials in parts by weight: 0.05-0.2 parts vitamin E, 0.05-0.2 parts curcumin, and 0.01-0.1 parts tea polyphenols.

[0027] By employing the above-mentioned approach, Vitamin E, as a fat-soluble antioxidant, captures free radicals through its phenolic hydroxyl groups, blocking the chain reaction of lipid oxidation. However, when used alone, it is easily degraded and rendered ineffective by high temperatures. Tea polyphenols contain highly active catechins, with antioxidant capacity 2-4 times that of BHA and BHT, and strong acid resistance, compensating for the thermal instability of Vitamin E. Curcumin has anti-inflammatory and lipid metabolism-regulating functions; its ketone structure can synergistically scavenge free radicals and alleviate oxidative stress damage by inhibiting the NF-κB pathway. The combination of these three components forms a multi-dimensional protection against free radical scavenging, enhanced thermal stability, and inflammation suppression, resulting in significantly higher antioxidant efficacy than any single ingredient. Furthermore, the combination of fat-soluble Vitamin E, water-soluble tea polyphenols, and amphiphilic curcumin creates a dual-phase system covering oil and water, enhancing the antioxidant coverage and increasing antioxidant activity. All ingredients in the formula are of natural origin and meet food and cosmetic safety standards, making it especially suitable for sensitive skin.

[0028] Preferably, the antioxidant is prepared from the following raw materials in parts by weight: 0.1-0.15 parts vitamin E, 0.1-0.15 parts curcumin, and 0.03-0.08 parts tea polyphenols.

[0029] Preferably, the antioxidant is prepared from the following raw materials in parts by weight: 0.1 parts vitamin E, 0.1 parts curcumin, and 0.05 parts tea polyphenols.

[0030] Secondly, this application provides a method for preparing a massage oil for dispelling cold and dampness, using the following technical solution:

[0031] S1 Raw Material Processing: Divide the compound Chinese medicine powder into a first and a second portion; divide the sesame oil into a first, a second, and a third portion for later use;

[0032] S2 First Extraction: The first portion of compound Chinese medicine powder is mixed with the first portion of sesame oil and heated for extraction. The second portion of compound Chinese medicine powder is mixed with the second portion of sesame oil and heated for extraction. After extraction, the mixtures are allowed to stand for 20-30 minutes to settle naturally, then filtered to obtain the first and second portions of first-extracted oil. The first and second portions of first-extracted oil are combined to obtain the combined first-extracted oil. The residue of the first and second portions of compound Chinese medicine powder after extraction is mixed with the residue of the second portion of compound Chinese medicine powder to obtain the mixed residue.

[0033] S3 Secondary Extraction: Mix the mixed residue with the third part of sesame oil and heat to extract. After extraction is complete, let it stand for 20-30 minutes to settle naturally to obtain the secondary extracted oil. Discard the residue.

[0034] S4 Medicinal Oil Emulsification: The combined first-extracted oil from step S2 and the second-extracted oil from step S3 are mixed to obtain medicinal oil; a certain amount of medicinal oil is weighed and heated in a water bath, the oil phase and antioxidant are added, stirred and kept warm, deionized water is added, heated in a water bath, emulsified with a homogenizer to form an emulsion, and cooled to room temperature for bottling.

[0035] By adopting the above-mentioned method, high-temperature extraction can significantly improve the solubility of sesame oil in the effective components of medicinal materials, accelerate the mass transfer rate, shorten the extraction time, reduce oil viscosity, and enhance permeability. At the same time, high-temperature extraction can soften or rupture plant cell walls and cell membranes, release intracellular fat-soluble components, improve the extraction rate, and also kill microorganisms in medicinal materials or oils, thus extending the shelf life of the product.

[0036] Preferably, in step S2, the initial extraction is performed by heating. Timing begins when the temperature reaches 100-150℃, and the extraction is carried out for 1-2 hours.

[0037] Preferably, in the secondary extraction in step S3, heating extraction is performed, and timing begins when the temperature reaches 100-150℃, with extraction maintained at this temperature for 1-2 hours.

[0038] Preferably, in step S4, the medicinal oil emulsification, the medicinal oil is heated to 70-90°C in a water bath, the oil phase and antioxidant are added, stirred for 10-20 minutes until evenly dispersed, and kept at 70-90°C. Deionized water is then added, and the mixture is heated to 70-90°C in a water bath.

[0039] Preferably, in step S4, the emulsification of the medicinal oil, the homogenizer performs emulsification through gradient homogenization: in the premixing stage, 1000-3000 rpm for 5-10 min; in the coarse emulsification stage, 5000-8000 rpm for 2-5 min; and in the high-speed homogenization stage, after cooling to below 40°C, 15000-18000 rpm for 3-8 min, until the system is uniformly milky white and free of visible particles.

[0040] By adopting the above scheme, homogenization emulsification is divided into three stages: premixing, coarse emulsification, and high-speed homogenization. Gradually increasing the homogenization pressure through gradient homogenization effectively improves homogenization efficiency, avoids the high-temperature damage to components and emulsification effects caused by long-term high-speed homogenization, enhances the mechanical stability of the emulsion, prevents stratification or demulsification, and improves the stability and uniformity of the emulsion. Cooling to below 40℃ before high-speed homogenization effectively avoids the degradation of active ingredients, such as volatile essential oils and heat-sensitive drugs, caused by high shear heat, ensuring the stability of the efficacy. The cold-dispelling and dampness-removing massage oil after gradient homogenization is milky white, free of particles, has good storage stability, and a long shelf life.

[0041] In summary, this application has the following beneficial effects:

[0042] 1. The massage oil prepared by this application for dispelling cold and dampness has the effects of clearing the meridians, dispelling cold and dampness, high transdermal efficiency, low irritation and good storage stability through the optimization of the natural penetration-enhancing system, the synergistic effect of multiple components of traditional Chinese medicine "principal, assistant, adjuvant and guide" and the innovation of the preparation process.

[0043] 2. The cold-dispelling and dampness-removing massage oil of this application utilizes the solubility of sesame oil in fat-soluble components, exhibiting a high solubility superior to water extraction or alcohol extraction. Simultaneously, the oil phase can isolate oxygen, reducing oxidative degradation. With sesame oil as the matrix, there is no need to separate the solvent after extraction, simplifying the process and ensuring high safety. Sesame oil as the matrix also more easily penetrates the skin's lipid barrier, acting as a natural penetration enhancer, effectively improving the transdermal absorption efficiency of the cold-dispelling and dampness-removing massage oil.

[0044] 3. This application discloses a massage oil for dispelling cold and dampness, in which the compound Chinese herbal powder has the core therapeutic principles of warming yang and dispelling cold, dispelling dampness and unblocking meridians, and promoting qi and blood circulation. This formula, with "warming and unblocking" as its core, achieves a synergistic effect of dispelling cold and dampness and promoting qi and unblocking meridians through a principal-assistant-adjuvant-guide structure of "principal medicine for dual external and internal dispelling, assistant medicine for penetrating meridians, adjuvant medicine for stabilizing, and guiding medicine for guiding the flow of qi." It also has the effects of inhibiting inflammation and promoting circulation.

[0045] 4. The cold-dispelling and dampness-removing massage oil of this application effectively destroys the cellulose structure by enzymatic hydrolysis of the broad-leaved vine powder in the compound Chinese medicine powder with cellulase, releasing small molecule triterpenoid active ingredients in the cells; the white mustard seed is roasted to reduce adverse skin irritation.

[0046] 5. The emulsifier oil phase of the cold-dispelling and dampness-removing massage oil of this application is a compound of sorbitol polyether-30 tetraoleate, cocamidopropyl betaine, tea saponin and sodium lauroyl sarcosinate. The mild design and multi-component synergy reduce the amount of synthetic emulsifier used, and achieve efficient emulsification and low skin irritation.

[0047] 6. The method for preparing a cold-dispelling and dampness-removing massage oil disclosed in this application utilizes high-temperature extraction, which significantly improves the solubility of sesame oil in the effective components of medicinal materials, accelerates mass transfer rate, shortens extraction time, reduces oil viscosity, and enhances permeability. Simultaneously, it can kill microorganisms in the medicinal materials or oil, extending the product's shelf life. Gradient homogenization emulsification gradually increases the homogenization pressure, effectively improving homogenization efficiency, avoiding the high-temperature damage to components and emulsification effect caused by long-term high-speed homogenization, and enhancing the mechanical stability of the emulsion. Detailed Implementation

[0048] The technical solution of this application is further illustrated by specific embodiments below. These specific embodiments do not represent a limitation on the scope of protection of this application. Any non-essential modifications and adjustments made by others based on the concept of this application still fall within the scope of protection of this application.

[0049] Unless otherwise specified, the experimental methods shown in the following examples are conventional methods. All reagents and materials shown are commercially available products.

[0050] This invention discloses a massage oil for dispelling cold and dampness. The compound herbal powder contains the following raw materials: Terminalia chebula, Cistanche deserticola, Aucklandia lappa, Benzoin, Nutmeg, Fennel, Asafoetida, White mustard seed, Safflower, Amomum tsao-ko, Cardamom, Clove, Sichuan pepper, and Nardostachys chinensis. These are mixed with sesame oil in a specific ratio to extract the effective components. Antioxidants, an oil phase, and deionized water are added for emulsification. The main components and efficacy of each part of the compound herbal powder are described below:

[0051] Terminalia chebula: also known as chebula lepidium or chebula suifengzi; taste and properties: bitter, sour, astringent, neutral; meridians: lung and large intestine; functions: astringes the intestines to stop diarrhea, astringes the lungs to stop cough, reduces fire and soothes the throat; indications: chronic diarrhea and dysentery, hematochezia and rectal prolapse; lung deficiency with wheezing and cough, persistent cough; sore throat and hoarseness.

[0052] *Vine of Broad Roots*: Also known as *Tinospora sinensis*, *Vine of Green*, and *Vine of Broad Roots*; Properties: Bitter, pungent, neutral. Channels Entered: Liver and Spleen. Traditional Functions: Dispels wind and dampness, relaxes muscles and tendons; used for rheumatic pain, joint contractures, and traumatic injuries.

[0053] Costus root: also known as costus, Guang costus, honey costus, and Nan costus; properties: pungent, bitter, and warm; meridians: spleen, stomach, large intestine, triple burner, and gallbladder; functions: promotes qi circulation and relieves pain, strengthens the spleen and aids digestion; indications: chest and rib pain, abdominal distension and pain, tenesmus after diarrhea; food stagnation and loss of appetite.

[0054] Benzoin: Also known as benzoin, white benzoin, and Siamese benzoin; Properties: pungent, bitter, neutral; Channels entered: heart, liver, and spleen. Functions: opens the orifices and awakens the mind, promotes qi and blood circulation, and relieves pain. Indications: stroke with phlegm syncope, epilepsy with coma; abdominal pain, postpartum hemorrhage; externally used to treat chronic ulcers that are slow to heal.

[0055] Nutmeg: Also known as fleshy fruit, jade fruit, top-end flesh, and kagule; Properties and Channels: pungent, warm; enters the spleen, stomach, and large intestine channels. Functions and Indications: warms the middle jiao and promotes qi circulation, astringes the intestines and stops diarrhea; used for spleen and stomach deficiency-cold, chronic diarrhea, abdominal distension and pain, poor appetite and vomiting, etc.

[0056] Fennel: Also known as small fennel, grain fennel, fragrant fennel seed, and Huai fennel; Functions and indications: dispels cold and relieves pain, regulates qi and harmonizes the stomach; used for symptoms such as abdominal pain due to cold hernia, testicular prolapse, abdominal distension and pain, poor appetite, vomiting and diarrhea, and dysmenorrhea. Properties and channels: pungent, warm; enters the liver, kidney, spleen, and stomach channels.

[0057] Asafoetida: also known as Xingqu, Xunqu, Haxini, Chou Asafoetida, and Wei Quji; properties and channels: pungent, bitter, warm; enters the spleen and stomach channels. Functions and indications: eliminates food stagnation, disperses abdominal masses, kills parasites; used for food stagnation, abdominal masses, abdominal pain due to intestinal parasites, malaria, dysentery, etc.

[0058] White mustard seed: also known as mustard seed, sage mustard, Sichuan mustard, and mustard seed; properties and channels: pungent, warm; enters the lung channel. Functions and indications: warms the lungs, resolves phlegm and promotes qi circulation, disperses nodules, unblocks the channels and relieves pain; used for symptoms such as cough with cold phlegm, chest and rib pain, numbness and pain in the joints due to phlegm stagnation in the channels, and carbuncles and boils.

[0059] Safflower: Also known as safflower, safflower, thorn safflower, safflower vegetable, yellow safflower; Properties and Channels: pungent, warm; enters the heart and liver channels. Functions and Indications: invigorates blood circulation and regulates menstruation, disperses blood stasis and relieves pain; used for amenorrhea, dysmenorrhea, lochia retention, abdominal masses, traumatic injuries, sores and swellings, etc.

[0060] Cardamom: Also known as safflower, red safflower, thorn safflower, red flower vegetable, yellow safflower; Properties and Channels: pungent, warm; enters the heart and liver channels. Functions and Indications: invigorates blood circulation and regulates menstruation, disperses blood stasis and relieves pain; used for amenorrhea, dysmenorrhea, lochia retention, abdominal masses, traumatic injuries, sores and swellings, etc.

[0061] Cardamom: Also known as white cardamom, round cardamom, multi-grain cardamom, purple cardamom, and wave cardamom; Properties and Channels: pungent, warm; enters the lung, spleen, and stomach channels. Functions and Indications: resolves dampness and promotes qi circulation, warms the middle jiao and stops vomiting, stimulates appetite and aids digestion; used for symptoms such as dampness obstructing the middle jiao, abdominal distension, loss of appetite, chest tightness and lack of hunger, vomiting, and hiccups.

[0062] Clove: Also known as male clove, clove bud, chicken tongue fragrance, male clove, and branch-shaped clove; Properties and Channels: pungent, warm; enters the spleen, stomach, lung, and kidney channels. Functions and Indications: warms the middle jiao and relieves nausea, tonifies the kidney and assists yang; used for symptoms such as vomiting due to stomach cold, hiccups, cold pain in the epigastrium and abdomen, impotence due to kidney deficiency, and infertility due to cold uterus.

[0063] Sichuan pepper: also known as Sichuan pepper, Qin pepper, Han pepper, Dianjiao, Dahongpao; properties and channels: pungent, warm; enters the spleen, stomach, and kidney channels. Functions and indications: warms the middle jiao and relieves pain, kills parasites and relieves itching; used for cold pain in the epigastrium and abdomen, vomiting and diarrhea, abdominal pain due to intestinal parasites; externally used for eczema, itching, vulvar itching and leukorrhea.

[0064] Nardostachys jatamansi: also known as sweet pine, fragrant pine, thyme, bitter pine, broad-leaved sweet pine; properties and channels: pungent, sweet, warm; enters the spleen and stomach channels. Functions and indications: regulates qi and relieves pain, invigorates the spleen and relieves depression; used for abdominal distension and pain, loss of appetite, and vomiting; externally used for toothache and beriberi swelling and pain.

[0065] The present application will be further described in detail below with reference to embodiments and comparative examples.

[0066] Preparation Example

[0067] Preparation Examples 1-3: Preparation of Tinospora sinensis powder

[0068] The powder of *Vitis thunbergii* was enzymatically hydrolyzed with cellulase using the following technical method: the powder of *Vitis thunbergii* was weighed, sterilized at 121℃ for 20 min to eliminate interference from endogenous enzymes, 0.05M citrate-disodium hydrogen phosphate buffer was added and mixed well, and the mixture was treated with a cellulase shaker to release small molecule triterpenes; the powder was centrifuged at 8000 rpm for 10 min, the hydrolyzed powder of *Vitis thunbergii* was collected, and dried with hot air at 100℃.

[0069] For 0.05M citrate-disodium hydrogen phosphate buffer, weigh 4.804g of citrate and 8.900g of disodium hydrogen phosphate, dissolve them in ultrapure water and bring the volume to 1L.

[0070] The amount of each component added and the preparation conditions during the preparation of *Vinealis macrocephala* powder by enzymatic hydrolysis with cellulase are shown in Table 1.

[0071] Table 1. Amounts of each component and preparation conditions in Preparation Examples 1-3

[0072]

[0073] Preparation of medicinal oils (Example 4-10)

[0074] The medicinal oil is a mixture of compound Chinese herbal powder and sesame oil in a certain proportion.

[0075] The compound Chinese medicine powder is prepared by weighing the following raw materials in proportion: Terminalia chebula, Tinospora sinensis powder, Aucklandia lappa, Benzoin, Nutmeg, Fennel, Asafoetida, White mustard seed, Safflower, Amomum tsao-ko, Cardamom, Clove, Sichuan pepper, and Nardostachys jatamansi.

[0076] White mustard seeds are stir-fried at 120-150℃ for 5-8 minutes until they turn dark yellow.

[0077] To obtain the compound Chinese medicine powder, pulverize each Chinese medicine raw material, pass it through a 40-80 mesh sieve, weigh it according to the proportion, and use a three-dimensional mixer for 20 minutes to obtain the compound Chinese medicine powder.

[0078] In preparation of Examples 4-10, each raw material and sesame oil in the compound Chinese medicine powder were weighed, and the compounding ratio and the amount of each component added are shown in Table 2.

[0079] Table 2. Proportions and weights of each component in the compound Chinese medicine powder prepared in Examples 4-10.

[0080]

[0081] Preparation of the oil phase in Examples 11-15

[0082] The oil phase is prepared from the following raw materials: sorbitol polyether-30 tetraoleate, cocamidopropyl betaine, tea saponin, and sodium lauroyl sarcosinate. The weighing of each raw material in the oil phase is shown in Table 3.

[0083] Table 3. Amounts (L) of each raw material added to the oil phase in Preparation Examples 11-15

[0084]

[0085] Preparation Examples 16-20: Preparation of Antioxidants

[0086] The antioxidant is prepared from the following raw materials: vitamin E, curcumin, and tea polyphenols. The weighing amounts of each raw material in the antioxidant are shown in Table 4.

[0087] Table 4. Amounts (L) of each raw material added in the antioxidants prepared in Examples 16-20

[0088]

[0089]

[0090] Preparation Example

[0091] Example 1

[0092] A method for preparing a massage oil for dispelling cold and dampness is provided, using the following technical solution:

[0093] S1 Raw Material Processing: Divide the compound Chinese medicine powder into a first and a second portion; divide the sesame oil into a first, a second, and a third portion; set aside.

[0094] S2 First Extraction: Mix the first portion of compound Chinese medicine powder with the first portion of sesame oil and start timing when the temperature reaches 100-150℃, keep warm and extract for 1-2 hours. Mix the second portion of compound Chinese medicine powder with the second portion of sesame oil and heat to extract; start timing when the temperature reaches 100-150℃, keep warm and extract for 1-2 hours. After extraction, let stand for 20-30 minutes for natural sedimentation and filtration to obtain the first and second portions of first-extracted oil. Combine the first and second portions of first-extracted oil to obtain the combined first-extracted oil. Mix the residue of the first and second portions of compound Chinese medicine powder after extraction to obtain the mixed residue.

[0095] S3 Secondary Extraction: Mix the mixed residue with the third part of sesame oil and heat to extract. Start timing when the temperature reaches 100-150℃ and keep it warm for 1-2 hours. After extraction is complete, let it stand for 20-30 minutes to settle naturally and obtain the secondary extracted oil. Discard the residue.

[0096] S4 Medicinal Oil Emulsification: Mix the combined first-extracted oil from step S2 and the second-extracted oil from step S3 to obtain a medicinal oil mixture. Weigh 6.0L of the mixture, heat it in a water bath to 70-90℃, add 1.86L of oil phase and 0.025L of antioxidant, stir for 10-20min until evenly dispersed, and keep it at 70-90℃. Add 4.0L of deionized water, heat it in a water bath to 70-90℃, and emulsify it using a homogenizer through gradient homogenization. In the premixing stage, homogenize at 1000-3000rpm for 5-10min; in the coarse emulsification stage, homogenize at 5000-8000rpm for 2-5min; in the high-speed homogenization stage, cool to below 40℃, homogenize at 15000-18000rpm for 3-8min, until the system is uniformly milky white and free of visible particles, forming an emulsion. Cool to room temperature and then bottle.

[0097] In the preparation of the medicinal oil, the compound Chinese medicine powder and sesame oil were prepared according to the method of Preparation Example 4; the emulsifier oil phase was prepared according to Preparation Example 11; and the antioxidant was prepared according to Preparation Example 16.

[0098] Example 2

[0099] A method for preparing a massage oil for dispelling cold and dampness is provided, which is the same as in Example 1, except that:

[0100] In the preparation of the medicinal oil, the compound Chinese medicine powder and sesame oil were prepared according to the method of Preparation Example 5; the emulsifier oil phase was prepared according to Preparation Example 12; and the antioxidant was prepared according to Preparation Example 17.

[0101] During the preparation process, 4.0L of medicinal oil, 1.7L of oil phase, 3.5L of deionized water, and 0.02L of antioxidant were taken respectively.

[0102] Example 3

[0103] A method for preparing a massage oil for dispelling cold and dampness is provided, which is the same as in Example 1, except that:

[0104] In the preparation of the medicinal oil, the compound Chinese medicine powder and sesame oil were prepared according to the method of Preparation Example 6; the emulsifier oil phase was prepared according to Preparation Example 13; and the antioxidant was prepared according to Preparation Example 18.

[0105] During the preparation process, 8.0L of medicinal oil, 1.9L of oil phase, 4.5L of deionized water, and 0.03L of antioxidant were taken respectively.

[0106] Example 4

[0107] A method for preparing a massage oil for dispelling cold and dampness is provided, which is the same as in Example 1, except that:

[0108] In the preparation of the medicinal oil, the compound Chinese medicine powder and sesame oil were prepared according to the method of Preparation Example 7; the emulsifier oil phase was prepared according to Preparation Example 14; and the antioxidant was prepared according to Preparation Example 19.

[0109] During the preparation process, 6.0L of medicinal oil, 1.8L of oil phase, 4.0L of deionized water, and 0.025L of antioxidant were taken respectively.

[0110] Example 5

[0111] A method for preparing a massage oil for dispelling cold and dampness is provided, which is the same as in Example 1, except that:

[0112] In the preparation of the medicinal oil, the compound Chinese medicine powder and sesame oil were prepared according to the method of Preparation Example 8; the emulsifier oil phase was prepared according to Preparation Example 15; and the antioxidant was prepared according to Preparation Example 20.

[0113] During the preparation process, 5.0L of medicinal oil, 1.7L of oil phase, 3.5L of deionized water, and 0.02L of antioxidant were taken respectively.

[0114] Example 6

[0115] A method for preparing a massage oil for dispelling cold and dampness is provided, which is the same as in Example 1, except that:

[0116] In the preparation of the medicinal oil, the compound Chinese medicine powder and sesame oil were prepared according to the method of Preparation Example 9; the emulsifier oil phase was prepared according to Preparation Example 12; and the antioxidant was prepared according to Preparation Example 16.

[0117] During the preparation process, 6.0L of medicinal oil, 1.8L of oil phase, 4.0L of deionized water, and 0.025L of antioxidant were taken respectively.

[0118] Example 7

[0119] A method for preparing a massage oil for dispelling cold and dampness is provided, which is the same as in Example 1, except that:

[0120] In the preparation of the medicinal oil, the compound Chinese medicine powder and sesame oil were prepared according to the method of Preparation Example 10; the emulsifier oil phase was prepared according to Preparation Example 14; and the antioxidant was prepared according to Preparation Example 18.

[0121] During the preparation process, 6.0L of medicinal oil, 1.8L of oil phase, 4.0L of deionized water, and 0.025L of antioxidant were taken respectively.

[0122] Comparative Example

[0123] Comparative Example 1

[0124] Similar to Example 1, except that the compound Chinese medicine powder is prepared from the following raw materials: 56g of Terminalia chebula, 77.2g of Aucklandia lappa, 85.2g of Benzoin, 238g of Myristica fragrans, 114g of Ferula assa-foetida, 79.2g of Sinapis alba, 23.2g of Carthamus tinctorius, 25g of Amomum tsao-ko, 58 parts of Zanthoxylum bungeanum, and 112g of Nardostachys jatamansi.

[0125] Comparative Example 2

[0126] Similar to Example 1, except that the compound Chinese medicine powder is prepared from the following raw materials: 80.6g of *Tinospora sinensis* powder, 77.2g of *Saussurea costus*, 85.2g of benzoin, 238g of nutmeg, 114g of *Ferula assa-foetida*, 43.2g of safflower, 25g of cardamom, 25g of nutmeg, 23.2g of cloves, and 58 parts of Sichuan pepper.

[0127] Comparative Example 3

[0128] Similar to Example 1, except that the compound Chinese medicine powder is prepared from the following raw materials: 56g of Terminalia chebula, 80g of Tinospora sinensis powder, 85.2g of benzoin, 258g of nutmeg, 118g of fennel, 114g of asafoetida, 79.2g of white mustard seed, 25g of cardamom, 23.2g of clove, 78 parts of Sichuan pepper, and 112g of Nardostachys jatamansi.

[0129] Comparative Example 4

[0130] Similar to Example 1, except that it was prepared using the following ingredients: 56g of Terminalia chebula, 69.6g of Tinospora sinensis, 77.2g of Aucklandia lappa, 85.2g of Benzoin, 238g of nutmeg, 118g of fennel, 114g of Ferula assa-foetida, 79.2g of white mustard seed, 23.2g of safflower, 25g of Amomum tsao-ko, 25g of cardamom, 23.2g of clove, 58g of Zanthoxylum bungeanum, and 112g of Nardostachys jatamansi. The Tinospora sinensis was not enzymatically hydrolyzed with cellulase, and the white mustard seed was not roasted.

[0131] Comparative Example 5

[0132] Same as Example 1, except that the oil phase is 1.2L of sorbitol polyether-30 tetraoleate and the deionized water is 2.8L.

[0133] Comparative Example 6

[0134] Same as Example 1, except that the oil phase is 1.2L cetearyl alcohol and the deionized water is 2.8L.

[0135] Comparative Example 7

[0136] Same as Example 1, except that the oil phase is 1.6L of sorbitol polyether-30 tetraoleate and 6L of deionized water.

[0137] Comparative Example 8

[0138] Same as Example 1, except that the oil phase is 1.46 L of sorbitol polyether-30 tetraoleate and 4 L of deionized water.

[0139] Comparative Example 9

[0140] Similar to Example 1, except that the oil phase is prepared by mixing 1.46 L of sorbitol polyether-30 tetraoleate and 0.16 L of cocamidopropyl betaine; and 4 L of deionized water.

[0141] Comparative Example 10

[0142] Similar to Example 1, except that the oil phase is prepared by mixing 1.46L of sorbitol polyether-30 tetraoleate and 0.25L of tea saponin; and 4L of deionized water.

[0143] Comparative Example 11

[0144] Similar to Example 1, except that the oil phase is prepared by mixing 1.46L of sorbitol polyether-30 tetraoleate, 0.16L of cocamidopropyl betaine and 0.15L of tea saponin; and 4L of deionized water.

[0145] Comparative Example 12

[0146] Same as Example 1, except that no antioxidants are added.

[0147] Comparative Example 13

[0148] Similar to Example 1, except that during the emulsification of the medicinal oil, the homogenization was performed at 10,000 rpm for 2-5 minutes, and then cooled to below 40°C for 15,000-18,000 rpm for 3-8 minutes.

[0149] Comparative Example 14

[0150] Similar to Example 1, except that during the emulsification of the medicinal oil, the homogenization was performed at 10,000 rpm for 10 minutes, and the homogenization was stopped every 2 minutes. The mixture was then cooled to below 40°C before homogenization was performed again.

[0151] Comparative Example 15

[0152] Similar to Example 1, except that the homogenization process for the medicinal oil emulsification was performed at 5000 rpm for 10 minutes.

[0153] Performance testing

[0154] 1. Stability test and results

[0155] Heat resistance stability: Take a small amount of the cold-dispelling and dampness-removing massage oil, seal it in a suitable container, and keep it in a 50℃ bath for 6 hours. Cold resistance stability: Place the sealed cream at -20℃ for 24 hours, then remove it and place it at room temperature. The evaluation standard is mainly based on whether the oil and water separate. After 5 days at room temperature, observe whether the surface of each cold-dispelling and dampness-removing massage oil has a glossy, uniform, and fine semi-solid state.

[0156] The cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 and Comparative Examples 1-14 were tested using the above methods, and the test results are shown in Table 5.

[0157] Table 5. Stability Test Results

[0158]

[0159]

[0160] As can be seen from the experimental data in Table 5, the cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 and Comparative Examples 1-4 and 12 do not separate into layers in heat-resistant and cold-resistant environments, and the oils also show good performance after being placed at room temperature, with a glossy, uniform, and delicate surface.

[0161] Comparative Examples 5-11 exhibited different states in each experiment. Under the same conditions, Comparative Examples 5 and 6 showed that sorbitol polyether-30 tetraoleate was more effective as an oil-phase emulsifier than cetearyl alcohol, and the process was more suitable. Therefore, sorbitol polyether-30 tetraoleate was selected as the primary emulsifier for subsequent experiments. In Comparative Example 7, the prepared cold-dispelling and dampness-removing massage oil showed stratification, with significant oil phase precipitation. Therefore, the oil-to-water ratio was reduced, and water-oil dispersion quickly occurred after emulsification. In Comparative Example 8, the amount of emulsifier added to the oil phase was further increased, and the resulting system was more stable than before. In Comparative Example 9, after determining the primary emulsifier and its amount, a co-emulsifier was considered to further improve the stability of the emulsion system. Comparative Example 10 showed slight stratification during cold-resistant storage, with a glossy, smooth, but uneven surface. Comparative Example 11 showed slight stratification during heat-resistant storage, with a glossy, smooth, and uniform surface. The oil phase of the emulsifier gradually showed a more stable effect through the compounding of the main emulsifier and the co-emulsifier.

[0162] In Comparative Example 13, homogenization was performed at 10,000 rpm for 2-5 minutes, followed by cooling to below 40°C and then homogenizing at 15,000-18,000 rpm for 3-8 minutes. The emulsification effect was poor, with slight stratification in both heat-resistant and cold-resistant environments. After being placed at room temperature, the surface of the medicinal oil was glossy and smooth, but uneven. In Comparative Example 14, homogenization was performed at 10,000 rpm for 10 minutes, with homogenization stopped every 2 minutes and then resumed after cooling to below 40°C. Although intermittent emulsification reduced the temperature rise under high shear forces, the emulsification effect remained poor. In Comparative Example 15, the medicinal oil emulsion was not completely emulsified and showed severe stratification.

[0163] Examples 1-7 show that the emulsion of cold-dispelling and dampness-removing massage oil, after emulsification, exhibits significantly improved stability compared to unemulsified oil. Simultaneously, the emulsification process disperses the cold-dispelling and dampness-removing massage oil into tiny droplets, significantly reducing particle size and increasing the contact area with the skin or mucous membranes. Poorly emulsified cold-dispelling and dampness-removing massage oil has large particle sizes, making it difficult to penetrate the stratum corneum, while emulsified droplets can penetrate rapidly, improving drug absorption efficiency. Cold-dispelling and dampness-removing massage oil with poor emulsification is easily oxidized or separated by temperature and light, while the interfacial film formed by the emulsifier can stabilize the droplets and prevent oil-water separation.

[0164] 2. Storage test and results

[0165] The condition and skin feel of each cold-dispelling and dampness-removing massage oil were observed after being left at room temperature for 18 months. The cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 and Comparative Example 12 were tested using the above method.

[0166] Through storage tests, the cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 and Comparative Example 12 remained stable for 6-18 months at room temperature. The cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 were milky white, had a refreshing feel, were easy to apply, left no residue, and retained a glossy, uniform, and fine semi-solid appearance. However, the cold-dispelling and dampness-removing massage oil prepared in Comparative Example 12 showed a slight yellowing color and a noticeable rancid odor after the third month, indicating that the oil would gradually decompose or oxidize during long-term storage, leading to rancidity.

[0167] 3. Allergy and efficacy tests and results

[0168] Allergy test: The cold-dispelling and dampness-removing massage oil was applied directly to the inside of the volunteers' arms. The volunteers were randomly selected from different age groups and genders, with 30 volunteers for each type of massage oil. After 48 hours of observation, the volunteers were investigated for any adverse reactions such as itching or allergies.

[0169] Efficacy test: Cold-dispelling and dampness-removing massage oils were applied to the affected areas of volunteers, who then gently massaged the areas with their palms for 10 minutes. Volunteers were randomly selected from different age groups and genders, with 30 volunteers for each type of massage oil. Test results were categorized into three levels: significantly effective, effective, and ineffective.

[0170] The cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 and Comparative Examples 1-4 were tested, and the results of allergy and efficacy tests were obtained, as shown in Table 6.

[0171] Table 6 Results of Allergy and Efficacy Tests

[0172]

[0173] As shown in Table 6, the cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 and Comparative Examples 1-4 were tested on 30 volunteers for each type of massage oil. In the allergy test, no allergic reactions were observed in Examples 1-7 and Comparative Examples 1-3. Only in Comparative Example 4 did 7 patients experience allergic reactions after application. This may be because the white mustard seeds in Comparative Example 4 were not roasted. The irritation of white mustard seeds mainly comes from the glucosinolates they contain. Under the action of myrosinase, glucosinolates decompose to generate allyl isothiocyanate, which causes local burning sensation and erythema on the skin. In severe cases, it can cause persistent redness and swelling, papules or vesicles, urticaria, and systemic itching.

[0174] In the efficacy test, it was clearly seen that patients who used the cold-dispelling and dampness-removing massage oils prepared in Examples 1-7 achieved significant and effective effects in both dispelling cold and dampness and unblocking meridians, with a large number of patients showing significant effects and good absorption. The cold-dispelling and dampness-removing massage oils in Examples 1-4 were not effective, with little effect on dispelling cold and dampness and unblocking meridians. The user experience was mostly focused on the two evaluation levels of effective and ineffective, and the absorption effect was slightly lower than that of Examples 1-7.

[0175] The above experimental results show that the cold-dispelling and dampness-removing massage oil prepared in this application, through the optimization of the natural penetration-enhancing system, the synergistic effect of multiple components of traditional Chinese medicine ("principal, assistant, adjuvant, and guide"), and the innovation of the preparation process, has the effects of unblocking meridians, dispelling cold and dampness, and has high transdermal efficiency, low irritation and good storage stability.

[0176] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but as long as they are within the scope of the present invention, they are protected by patent law.

Claims

1. A massage oil for dispelling cold and dampness, characterized in that, It is prepared from the following raw materials in parts by weight: 40-80 parts of medicinal oil, 17-19 parts of oil phase, 35-45 parts of deionized water and 0.2-0.3 parts of antioxidant; The medicinal oil is a compound Chinese medicine powder and sesame oil in a mass ratio of 1:(8-10). The compound Chinese medicine powder is made from the following raw materials in parts by weight: 20-35 parts of Terminalia chebula, 25-40 parts of Tinospora sinensis powder, 34-43 parts of Aucklandia lappa, 37-46 parts of Benzoin, 110-125 parts of Myristica fragrans, 54-63 parts of Fennel, 54-63 parts of Ferula assa-foetida, 36-42 parts of Sinapis alba, 7-16 parts of Carthamus tinctorius, 9-15 parts of Amomum tsao-ko, 7-16 parts of Amomum cardamomum, 9-15 parts of Clove, 27-36 parts of Zanthoxylum bungeanum, and 50-65 parts of Nardostachys jatamansi. The oil phase is prepared from the following raw materials in parts by weight: 11-17 parts of sorbitol polyether-30 tetraoleate, 1-2.5 parts of cocamidopropyl betaine, 1-2.5 parts of tea saponin, and 0.5-1.5 parts of sodium lauroyl sarcosinate. The massage oil for dispelling cold and dampness is prepared by the following steps: S1 Raw material processing: Divide the compound Chinese medicine powder into a first and a second portion; divide the sesame oil into a first, a second, and a third portion, and set aside. S2 First extraction: Mix the first part of compound Chinese medicine powder with the first part of sesame oil and heat to extract; mix the second part of compound Chinese medicine powder with the second part of sesame oil and heat to extract; after extraction, let stand for 20-30 minutes to settle naturally and then filter to obtain the first part of first extracted oil and the second part of first extracted oil. Combine the first part of first extracted oil and the second part of first extracted oil to obtain the combined first extracted oil. The residue of the first part of the compound Chinese medicine powder after extraction is mixed with the residue of the second part of the compound Chinese medicine powder to obtain a mixed residue; S3 Secondary extraction: Mix the mixed residue with the third part of sesame oil and heat to extract. After extraction is complete, let it stand for 20-30 minutes to settle naturally to obtain the secondary extracted oil. Discard the residue. S4 Medicinal Oil Emulsification: The combined first-extracted oil from step S2 and the second-extracted oil from step S3 are mixed to obtain medicinal oil; a certain amount of medicinal oil is weighed and heated in a water bath, the oil phase and antioxidant are added, stirred and kept warm, deionized water is added, heated in a water bath, emulsified with a homogenizer to form an emulsion, and cooled to room temperature for bottling. The powder of *Vitis thunbergii* was enzymatically hydrolyzed with cellulase: the powder of *Vitis thunbergii* was weighed, sterilized at 121℃ for 20 min to eliminate interference from endogenous enzymes, mixed with buffer solution, and treated with cellulase at 40-60℃ and 130-180 rpm for 2-4 h to release small molecule triterpenes. After centrifugation at 3000 rpm for 5 min, the hydrolyzed powder of *Vitis thunbergii* was collected and dried with hot air at 80-120℃ for 0.5-2 h. The white mustard seeds are stir-fried at 120-150℃ for 5-8 minutes until they turn a deep yellow color.

2. The cold-dispelling and dampness-removing massage oil according to claim 1, characterized in that, The powder of *Vitis thunbergii* was hydrolyzed with cellulase, and the mass ratio of *Vitis thunbergii* powder, buffer solution and cellulase was 5:(18-22):

1.

3. The cold-dispelling and dampness-removing massage oil according to claim 1, characterized in that, The antioxidant is prepared from the following raw materials in parts by weight: 0.05-0.2 parts vitamin E, 0.05-0.2 parts curcumin and 0.01-0.1 parts tea polyphenols.

4. The preparation method of the cold-dispelling and dampness-removing massage oil according to claim 1, characterized in that: In the initial extraction of step S2, timing begins when the temperature reaches 100-150℃, and the extraction is carried out for 1-2 hours.

5. The preparation method of the cold-dispelling and dampness-removing massage oil according to claim 1, characterized in that: In step S3, the secondary extraction is performed by heating. When the temperature reaches 100-150℃, the timing begins, and the extraction is carried out for 1-2 hours.

6. The method for preparing the cold-dispelling and dampness-removing massage oil according to claim 1, characterized in that: In step S4, the emulsification of the medicinal oil, the homogenizer performs emulsification through gradient homogenization: premixing stage, 1000-3000 rpm, homogenization for 5-10 minutes; coarse emulsification stage, 5000-8000 rpm, homogenization for 2-5 minutes; high-speed homogenization stage, cooled to below 40°C, 15000-18000 rpm, homogenization for 3-8 minutes.

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