Layered stem cell incubator

Abstract

The present application relates to stem cell culture technical field, specifically to a layered stem cell incubator, including: incubator body, the incubator body is provided with the cavity, the cavity opening is hinged with the door, still including: limiting mechanism and anti -drop mechanism, limiting mechanism is used for limiting the circular vessel, its limiting can effectively avoid in carrying, accessing the damage of culture box article, because the damage of vessel sliding or falling; Anti -drop mechanism is used for preventing layered placement board from falling when drawing, prevents the layered placement board from separating from the cavity of incubator body when drawing too fast, when the experimental personnel draw the layered placement board, the connecting shaft slides along the anti -drop groove, when stopping, automatically embedded in the corrugated groove of anti -drop groove, avoid because of too violent or misoperation leads to layered board completely separate from the cavity, prevent the damage of culture vessel falling.

Description

Technical Field

[0001] This invention relates to the field of stem cell culture technology, specifically a layered stem cell culture box. Background Technology

[0002] Stem cell culture incubators are specialized devices used to maintain the survival, proliferation, and differentiation of stem cells. By precisely controlling environmental parameters such as temperature, humidity, and gas concentration, they simulate the physiological environment in vivo, ensuring the stability of stem cell biological characteristics. Stem cell culture incubators are core equipment in fields such as basic stem cell research, clinical treatment, and drug development. After connecting the power supply to the device, set the temperature and CO2 concentration, then place the culture dishes lined with matrix gel into the incubator for pre-incubation. When reviving cells, quickly thaw the cryopreservation tubes, centrifuge, resuspend them in complete culture medium, and then inoculate them into pre-treated culture dishes.

[0003] Generally, round plastic vessels are chosen for stem cell culture because they are suitable for cell growth and proliferation, and especially convenient for observing and selecting single-cell colonies. Their large openings make them easy to handle; the vessels are placed directly on the rack and then pushed into the incubator. However, because the contact area between the round vessel and the rack is small, uneven force or excessive speed during insertion can easily cause the vessel to tilt, slide, or even spill the culture medium, potentially leading to contamination or cell damage. Furthermore, the rack is secured to the incubator by protruding retaining strips. Excessive force when pulling the rack can cause it to detach rapidly from the incubator's placement chamber, resulting in violent shaking of the vessel due to inertia. This can cause the culture medium to spill outside the vessel or even into the incubator. Spilled culture medium can not only contaminate other samples but also cause cell loss or changes in local osmotic pressure, affecting stem cell viability. Summary of the Invention

[0004] The purpose of this invention is to provide a layered stem cell culture box to solve the problems mentioned in the background art.

[0005] The technical solution adopted by this application to solve its technical problem is: a layered stem cell culture box, comprising: a culture box body, wherein the culture box body is provided with a placement cavity, and a door is hingedly provided at the opening of the placement cavity, and further comprising:

[0006] A limiting mechanism is provided inside the placement cavity. The limiting mechanism includes layered placement plates that are equidistantly arranged inside the placement cavity. A limiting plate 1 and a limiting plate 2 are provided on the layered placement plates. The limiting mechanism is used to limit the culture vessel.

[0007] An anti-drop mechanism is provided on both sides of the limiting mechanism. The anti-drop mechanism includes fixing strips at the bottom of both sides of the layered placement plate. The top of the fixing strips has a slot, and the bottom of the layered placement plate slides in the slot. The anti-drop mechanism is used to prevent the layered placement plate from falling off when it is pulled out.

[0008] Preferably, a ventilation opening is provided on one side of the incubator body, a support is fixedly provided at the bottom of the incubator body, a sealing strip is fixedly provided on one side of the placement cavity opening, a handle is fixedly provided on one side of the door, and a control panel is provided on the side of the door near the handle.

[0009] Preferably, the limiting mechanism further includes a straight slot through which the layered placement plate is opened, a fixed shaft is fixedly provided on the top of the layered placement plate, a rotating disk is rotatably provided on the outer surface of the fixed shaft, and there is a resistance to the rotation between the rotating disk and the fixed shaft.

[0010] Preferably, a fixed plate is fixedly provided on the top of the rotating disk, and a limiting groove is provided at the bottom of the fixed plate. One part of the limiting groove is recessed towards the center of the fixed plate, and a roller is slidably provided in the limiting groove, with the bottom of the roller extending out of the limiting groove.

[0011] Preferably, the rotating disk is fixedly provided with multiple sets of guide rails symmetrically arranged, the multiple sets of guide rails are arranged in a circular array, the guide rails are fixedly connected to the first limiting disk, and the guide rails are slidably connected to the second limiting disk.

[0012] Preferably, both the first limiting disk and the second limiting disk have through-cut arc-shaped grooves, and the arc-shaped grooves on the first limiting disk and the second limiting disk are arranged opposite to each other. A connecting rod is fixedly provided on one side of the second limiting disk, and the end of the connecting rod away from the second limiting disk is fixedly connected to the roller shaft.

[0013] Preferably, the anti-fall-off mechanism further includes a groove formed on the fixing strip, the groove being interconnected with the slot, a sliding strip being provided in the groove, and the top of the sliding strip being fixedly connected to the bottom of the layered placement plate.

[0014] Preferably, the sliding bar is symmetrically fixed with connecting bars, the sliding bar slides in the groove through the connecting bars, and the fixed bar is symmetrically provided with anti-detachment grooves.

[0015] Preferably, a connecting shaft is symmetrically fixed on the sliding bar, a connecting piece is rotatably mounted on the connecting shaft, a movable shaft is fixedly mounted at the end of the connecting piece away from the connecting shaft, and the movable shaft is slidably mounted in the anti-detachment groove.

[0016] Preferably, each of the connecting pieces has a groove, and each of the grooves has a notch on one side. Each of the grooves has an elastic piece that is movably disposed in the groove. Each elastic piece has a fixing block fixedly disposed on both sides of the end away from the groove, and the fixing block is fixedly connected to the side wall of the connecting piece.

[0017] The beneficial effects of this application are:

[0018] This application provides a layered stem cell culture box that positions the culture dish between the arc-shaped grooves of limiting plate one and limiting plate two. This limiting mechanism allows researchers to quickly and accurately place the culture dish in the designated position without repeated adjustments, improving operational efficiency. The secure positioning of the dish effectively prevents damage caused by slipping or falling of the dish during handling and retrieval of items within the culture box, protecting valuable stem cell samples and experimental data.

[0019] This application provides a layered stem cell culture box. When the layered placement plate is pulled along the straight groove, a sliding bar moves the moving shaft within the anti-detachment groove. A connecting piece, via an elastic plate, presses against the notch, causing the connecting shaft to engage within the continuous corrugated section of the anti-detachment groove. When pulling stops, the position of the sliding bar is limited by the connecting shaft engaging within the anti-detachment groove, maintaining a relatively fixed position. This prevents the layered placement plate from detaching from the culture box's placement cavity if pulled too quickly. The continuous corrugated section and the connecting shaft create a physical locking effect. When the experimenter pulls the layered placement plate, the connecting shaft slides along the anti-detachment groove and automatically engages within the corrugated groove when stopped, preventing the layered plate from completely detaching from the placement cavity due to excessive force or misoperation, thus preventing the culture vessels from falling and being damaged.

[0020] In addition to the purposes, features, and advantages described above, this application has other purposes, features, and advantages. These will be further described in detail below with reference to figures. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the internal structure of the placement cavity of the present invention;

[0023] Figure 3 This is a schematic diagram of the cross-sectional structure of the incubator body of the present invention;

[0024] Figure 4 This is a schematic diagram of the cross-sectional structure of the fixed disk of the present invention;

[0025] Figure 5 This is an exploded view of the limiting mechanism of the present invention;

[0026] Figure 6This is a schematic diagram of the cross-sectional structure of the incubator sidewall of the present invention;

[0027] Figure 7 For the present invention Figure 6 Enlarged structural diagram at point A;

[0028] Figure 8 This is a schematic diagram of the anti-detachment mechanism of the present invention.

[0029] Drawing number explanation:

[0030] 1. Incubator body; 2. Placement chamber; 3. Door; 4. Ventilation opening; 5. Support; 6. Sealing strip; 7. Handle; 8. Control panel; 9. Limiting mechanism; 10. Layered placement plate; 11. Straight groove; 12. Fixed shaft; 13. Fixed plate; 14. Limiting groove; 15. Roller shaft; 16. Rotating plate; 17. Guide rail; 18. Limiting plate one; 19. Limiting plate two; 20. Arc groove; 21. Connecting rod; 22. Anti-falling mechanism; 23. Fixing strip; 24. Slot; 25. Sliding groove; 26. Sliding strip; 27. Connecting strip; 28. Anti-falling groove; 29. ​​Connecting shaft; 30. Connecting piece; 31. Moving shaft; 32. Groove; 33. Notch; 34. Elastic piece; 35. Fixing block. Detailed Implementation

[0031] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0032] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present application.

[0033] Please refer to Figures 1 to 8A layered stem cell culture chamber includes: a culture chamber body 1, a placement cavity 2, a door 3 hinged to the opening of the placement cavity 2, a ventilation opening 4 on one side of the culture chamber body 1, and a removable filter screen installed at the ventilation opening 4 to effectively filter impurities and microorganisms in the outside air and prevent contamination of the culture environment; a support 5 made of non-slip rubber is fixedly installed at the bottom of the culture chamber body 1 to enhance the stability of the equipment and effectively reduce vibration transmission during operation; a sealing strip 6 is fixedly installed on one side of the opening of the placement cavity 2 to form a tight seal when the door 3 is closed, preventing the intrusion of external air and leakage of the internal culture environment; a handle 7 is fixedly installed on one side of the door 3, and a control panel 8 is installed on the side of the door 3 near the handle 7.

[0034] It should be added that the incubator body 1 is also equipped with a temperature control system, including a temperature sensor, a heating element, and a cooling element. The temperature sensor monitors the temperature inside the placement chamber 2 in real time and feeds the data back to the control panel 8. The control panel 8 automatically adjusts the working status of the heating element or the cooling element according to the preset temperature value to maintain a stable incubation temperature.

[0035] Please refer to the details. Figures 1 to 6 A layered stem cell culture chamber further includes a limiting mechanism 9, which is disposed within a placement cavity 2. The limiting mechanism 9 includes layered placement plates 10 equidistantly arranged within the placement cavity 2. Each layered placement plate 10 is equipped with a limiting disc 18 and a limiting disc 19. The limiting mechanism 9 is used to limit the position of the culture vessels. Stem cell culture requires extremely high environmental stability. The limiting mechanism 9 prevents the culture vessels from shifting due to vibrations during chamber operation or the inertia of opening the door, avoiding shaking or even spillage of the culture medium, and maintaining the stable microenvironment required for cell growth. Limiting the position reduces the risk of contact between the vessels and the outside environment, such as preventing collisions with other components when the chamber door 3 is closed due to vessel shaking, thereby reducing the possibility of external contamination and ensuring the cleanliness of the culture environment.

[0036] The limiting mechanism 9 also includes a straight slot 11 that passes through the layered placement plate 10. Through the straight slot 11, an operator can pull the layered placement plate 10 out of the placement cavity 2. A fixed shaft 12 is fixedly installed at the top of the layered placement plate 10. A rotating disk 16 is rotatably mounted on the outer surface of the fixed shaft 12. There is resistance between the rotating disk 16 and the fixed shaft 12 during rotation. A fixed disk 13 is fixedly installed at the top of the rotating disk 16. A limiting groove 14 is formed at the bottom of the fixed disk 13. One section of the limiting groove 14 is recessed towards the center of the fixed disk 13. A roller 15 is slidably mounted within the limiting groove 14, with the bottom of the roller 15 extending out of the limiting groove 14.

[0037] It is worth mentioning that multiple sets of guide rails 17 are symmetrically arranged on the rotating disk 16. The multiple sets of guide rails 17 are arranged in a circular array. The guide rails 17 are fixedly connected to the first limiting disk 18, and the guide rails 17 are slidably connected to the second limiting disk 19. Both the first limiting disk 18 and the second limiting disk 19 have arc-shaped grooves 20 through them. The arc-shaped grooves 20 on the first limiting disk 18 and the second limiting disk 19 are arranged opposite to each other. A connecting rod 21 is fixedly installed on one side of the second limiting disk 19. The end of the connecting rod 21 away from the second limiting disk 19 is fixedly connected to the roller shaft 15.

[0038] By rotating the rotating disk 16 along the fixed axis 12, a relative displacement is generated between the rotating disk 16 and the fixed disk 13. When the rotating disk 16 rotates, the limiting groove 14 in the fixed disk 13 drives one of the rollers 15 to move towards the center of the fixed disk 13. During this process, the roller 15 pulls the limiting disk 19 away from the limiting disk 19 through the connecting rod 21. At this time, the culture dish can be placed between the arc groove 20 of the limiting disk 18 and the limiting disk 19, so that the culture dish is close to the arc groove 20 of the limiting disk 18. When the rotating disk 16 rotates again, under the restriction of the limiting groove 14, the roller 15 pushes the limiting disk 19 closer to and fits the limiting disk 18 through the connecting rod 21, thus limiting the culture dish. The experimenter can quickly and accurately place the culture dish in the designated position through the limiting mechanism 9 without repeated adjustments, thus improving the operating efficiency.

[0039] Please refer to the details. Figure 2 , Figure 4 as well as Figures 6 to 8 A layered stem cell culture box further includes: an anti-detachment mechanism 22, which is disposed on both sides of a limiting mechanism 9. The anti-detachment mechanism 22 includes fixing strips 23 disposed at the bottom ends of both sides of the layered placement plate 10. The top of the fixing strips 23 has a slot 24, and the bottom of the layered placement plate 10 slides within the slot 24. The anti-detachment mechanism 22 is used to prevent the layered placement plate 10 from falling off when pulled out. The anti-detachment mechanism 22 also includes a sliding groove 25 formed on the fixing strips 23, which is connected to the slot 24. A sliding strip 26 is disposed within the sliding groove 25, and the top of the sliding strip 26 is fixedly connected to the bottom of the layered placement plate 10.

[0040] A connecting strip 27 is symmetrically fixedly mounted on the sliding strip 26. The sliding strip 26 slides within the groove 25 via the connecting strip 27. A fixed strip 23 has symmetrically provided anti-detachment grooves 28. A connecting shaft 29 is symmetrically fixedly mounted on the sliding strip 26. A connecting piece 30 is rotatably mounted on the connecting shaft 29. A movable shaft 31 is fixedly mounted at the end of the connecting piece 30 away from the connecting shaft 29. The movable shaft 31 slides within the anti-detachment groove 28. Grooves 32 are provided on each connecting piece 30. A notch 33 is provided on one side of each groove 32. An elastic piece 34 is movably mounted within each groove 32. Fixing blocks 35 are fixedly mounted on both sides of the end of the elastic piece 34 away from the groove 32. The fixing blocks 35 are fixedly connected to the sidewall of the connecting piece 30.

[0041] When the layered placement plate 10 needs to be pulled out to place the culture dish, the experimenter can pull the layered placement plate 10 through the straight groove 11. At this time, the sliding bar 26 will pull the moving shaft 31 to move into the anti-detachment groove 28. The connecting piece 30 is pressed against the notch 33 by the elastic piece 34, causing the connecting shaft 29 to be locked in the continuous corrugated section of the anti-detachment groove 28. When pulling stops, the position of the sliding bar 26 can be limited by the connecting shaft 29 being locked in the anti-detachment groove 28, and its position remains relatively fixed to prevent the layered placement plate 10 from detaching from the placement cavity 2 of the incubator body 1 when pulled too quickly. The continuous corrugated section and the connecting shaft 29 form a physical locking effect. When the experimenter pulls the layered placement plate 10, the connecting shaft 29 slides along the anti-detachment groove 28, and automatically embeds into the corrugated groove of the anti-detachment groove 28 when stopped, avoiding the layered plate from completely detaching from the placement cavity 2 due to excessive force or misoperation, and preventing the culture dish from falling and being damaged.

[0042] The working principle of this invention is as follows:

[0043] The experimenter opens the door 3 and pulls the layered placement plate 10 through the straight groove 11. At this time, the sliding bar 26 pulls the moving shaft 31 into the anti-detachment groove 28. The connecting piece 30 is pressed against the notch 33 by the elastic piece 34, causing the connecting shaft 29 to be locked in the continuous corrugated section of the anti-detachment groove 28. When pulling stops, the position of the sliding bar 26 can be limited by the connecting shaft 29 being locked in the anti-detachment groove 28, and its position remains relatively fixed to prevent the layered placement plate 10 from detaching from the placement cavity 2 of the incubator body 1 when pulled too quickly. When the experimenter pulls the layered placement plate 10, the connecting shaft 29 slides along the anti-detachment groove 28, and automatically embeds into the corrugated groove of the anti-detachment groove 28 when stopped, to avoid the layered plate completely detaching from the placement cavity 2 due to excessive force or misoperation, and to prevent the culture vessels from falling and being damaged. Then, by rotating the rotating disk 16 along the fixed shaft 12, when When the rotating disk 16 rotates, a relative displacement occurs between it and the fixed disk 13. The limiting groove 14 in the fixed disk 13 drives one of the rollers 15 to move towards the center of the fixed disk 13 when the rotating disk 16 rotates. During this process, the roller 15 pulls the limiting disk 19 away from the fixed disk 13 through the connecting rod 21. At this time, the culture dish can be placed between the arc groove 20 of the limiting disk 18 and the limiting disk 19, so that the culture dish is close to the arc groove 20 of the limiting disk 18. When the rotating disk 16 rotates again, under the restriction of the limiting groove 14, the roller 15 pushes the limiting disk 19 closer to and fits the limiting disk 18 through the connecting rod 21, thus limiting the circular culture dish. The experimenter can quickly and accurately place the culture dish in the designated position through the limiting mechanism 9 without repeated adjustments, thus improving the operating efficiency. Securely holding the containers in place effectively prevents damage caused by the containers sliding or falling during handling and retrieval of items inside the incubator, thus protecting precious stem cell samples and experimental data.

[0044] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary. Under the framework of this invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.

[0045] This invention is intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A layered stem cell culture box, comprising: The incubator body (1) is provided with a placement cavity (2), and a door (3) is hinged at the opening of the placement cavity (2). The incubator body (1) is characterized by further comprising: The limiting mechanism (9) is disposed in the placement cavity (2). The limiting mechanism (9) includes a layered placement plate (10) equidistantly disposed in the placement cavity (2). The layered placement plate (10) is provided with a limiting disk one (18) and a limiting disk two (19). The limiting mechanism (9) is used to limit the culture vessel. The limiting mechanism (9) also includes a straight groove (11) that passes through the layered placement plate (10). A fixed shaft (12) is fixedly disposed on the top of the layered placement plate (10). A rotating disk (16) is rotatably disposed on the outer surface of the fixed shaft (12). There is a resistance to the rotation between the rotating disk (16) and the fixed shaft (12). An anti-drop mechanism (22) is provided on both sides of the limiting mechanism (9). The anti-drop mechanism (22) includes fixing strips (23) provided at the bottom ends of both sides of the layered placement plate (10). The top of the fixing strips (23) is provided with a slot (24). The bottom of the layered placement plate (10) slides in the slot (24). The anti-drop mechanism (22) is used to prevent the layered placement plate (10) from falling off when it is pulled out. The anti-drop mechanism (22) also includes a slot provided on the bottom end of the layered placement plate (10). The fixed strip (23) has a sliding groove (25) that is connected to the slot (24). A sliding strip (26) is provided in the sliding groove (25). The top of the sliding strip (26) is fixedly connected to the bottom of the layered placement plate (10). A connecting strip (27) is symmetrically fixed on the sliding strip (26). The sliding strip (26) slides in the sliding groove (25) through the connecting strip (27). Anti-detachment grooves (28) are symmetrically opened through the fixed strip (23).

2. The layered stem cell culture box according to claim 1, characterized in that, The incubator body (1) has a ventilation opening (4) on one side, a support (5) is fixedly installed at the bottom of the incubator body (1), a sealing strip (6) is fixedly installed on one side of the opening of the placement cavity (2), a handle (7) is fixedly installed on one side of the door (3), and a control panel (8) is installed on the side of the door (3) near the handle (7).

3. The layered stem cell culture box according to claim 1, characterized in that, A fixed disk (13) is fixedly installed on the top of the rotating disk (16). A limiting groove (14) is opened at the bottom of the fixed disk (13). One part of the limiting groove (14) is recessed towards the center of the fixed disk (13). A roller (15) is slidably installed in the limiting groove (14). The bottom of the roller (15) extends out of the limiting groove (14).

4. A layered stem cell culture box according to claim 3, characterized in that, The rotating disk (16) is fixedly provided with multiple sets of guide rails (17) arranged symmetrically. The multiple sets of guide rails (17) are arranged in a circular array. The guide rails (17) are fixedly connected to the first limiting disk (18), and the guide rails (17) are slidably connected to the second limiting disk (19).

5. A layered stem cell culture box according to claim 4, characterized in that, Both the first limiting plate (18) and the second limiting plate (19) are provided with arc-shaped grooves (20). The arc-shaped grooves (20) on the first limiting plate (18) and the second limiting plate (19) are arranged opposite to each other. A connecting rod (21) is fixedly provided on one side of the second limiting plate (19). The end of the connecting rod (21) away from the second limiting plate (19) is fixedly connected to the roller shaft (15).

6. A layered stem cell culture box according to claim 1, characterized in that, A connecting shaft (29) is symmetrically fixed on the sliding bar (26), and a connecting piece (30) is rotatably mounted on the connecting shaft (29). A movable shaft (31) is fixedly mounted on the end of the connecting piece (30) away from the connecting shaft (29), and the movable shaft (31) is slidably mounted in the anti-detachment groove (28).

7. A layered stem cell culture box according to claim 6, characterized in that, The connecting piece (30) is provided with grooves (32), and a notch (33) is provided on one side of the groove (32) on the connecting piece (30). An elastic piece (34) is movably arranged in the groove (32). A fixing block (35) is fixedly arranged on both sides of the end of the elastic piece (34) away from the groove (32). The fixing block (35) is fixedly connected to the side wall of the connecting piece (30).

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