Precise antibody screening equipment for phage antibody library

Abstract

The invention relates to the technical field of antibody screening equipment, in particular to precise antibody screening equipment for a phage antibody library, which comprises an equipment shell, a screening assembly is mounted in the equipment shell, the screening assembly comprises a mixing barrel, a stirring rod is rotatably mounted in the mixing barrel, one end of the stirring rod is connected with a blocking block, and the other end of the stirring rod is connected with a connecting rod. A one-way gear set is installed at the other end of the stirring rod, a rotating rod is connected to one end of the one-way gear set, a centrifugal barrel is installed at the lower end of the rotating rod in a transmission mode, a water pump is fixedly installed in the centrifugal barrel, and an auxiliary assembly is installed on the equipment shell; a constructor is externally connected with an air conditioner through a first spiral pipe access port, cold air surrounds the outside of the centrifugal barrel through the first spiral pipe and then is discharged through an adjusting pipeline and a first multi-way pipe, the centrifugal barrel absorbs the cold air for cooling, the temperature is maintained within a certain range, and the situation that phage particles are disintegrated due to the too high centrifugal temperature is avoided; the antibody display and screening results are influenced.

Description

Technical Field

[0001] The present invention relates to the technical field of antibody screening equipment, and in particular to a precise antibody screening equipment for phage antibody libraries. Background Art

[0002] Precision antibody screening equipment for phage antibody libraries is designed to efficiently and accurately screen antibody molecules with specific binding properties from a large phage antibody library. Phage antibody library technology involves cloning the variable region gene of an antibody into a phage vector, displaying the antibody on the phage surface as a fusion protein, and screening the target antibody through specific binding to the target antigen. Precision antibody screening equipment uses a series of technical means to improve the sensitivity, specificity, and efficiency of screening, providing high-quality antibody molecules for antibody drug development, disease diagnosis and other fields.

[0003] Currently, during the centrifugation process of phage antibody library screening antibodies, the centrifugation of the phage antibody library and the elution mixed solution needs to limit and consider its temperature range. If the temperature is too high and exceeds the temperature range required for centrifugation, it is easy to cause the phage particles to disintegrate, resulting in the antibodies in the phage antibody library unable to be normally displayed on the phage surface, thereby affecting the screening results. Summary of the Invention

[0004] The purpose of the present invention is to provide a precise antibody screening device for a phage antibody library. Construction personnel connect an external air conditioner through a first spiral tube inlet. The cold air surrounds the outside of the centrifuge barrel through the first spiral tube and is then discharged through a regulating pipe and a first multi-way pipe. The centrifuge barrel absorbs the cold air to cool down and maintain the temperature within a certain range, thereby preventing the centrifugal temperature from being too high and causing the phage particles to disintegrate, affecting the antibody display and screening results.

[0005] To achieve the above objectives, the present invention provides the following technical solutions: a precision antibody screening device for a phage antibody library, comprising a device housing, a screening assembly installed inside the device housing, the screening assembly including a mixing barrel, a stirring rod rotatably installed inside the mixing barrel, one end of the stirring rod being connected to a block, the other end of the stirring rod being installed with a one-way gear set, one end of the one-way gear set being connected to a rotating rod, a centrifugal bucket being transmission-mounted at the lower end of the rotating rod, and a water pump being fixedly installed inside the centrifugal bucket;

[0006] An auxiliary component is installed on the equipment casing, and the auxiliary component includes a first spiral tube, the first spiral tube is fixedly installed on the outside of the centrifugal barrel, one side of the first spiral tube is fixedly connected to an adjusting pipe, a scale tube is fixedly installed inside the adjusting pipe, a first push rod is slidably installed inside the scale tube, one side of the adjusting pipe is fixedly connected to a second spiral tube, the second spiral tube is fixedly installed on the outside of the mixing barrel, one side of the adjusting pipe is fixedly connected to a first multi-way tube, one side of the first multi-way tube is fixedly connected to an air collecting box, one side of the air collecting box is fixedly connected to a filter box, one side of the filter box is fixedly connected to a water pump, a gel filter screen is slidably installed inside the filter box, and a striking frame is rotatably installed inside the filter box.

[0007] Preferably, one end of the blocking block is fixedly connected to a first spring, a telescopic sleeve is provided on the outside of the first spring, one end of the first spring is fixedly connected to a fixing bracket, and the fixing bracket is fixedly installed on the device housing.

[0008] Preferably, one end of the one-way gear set is fixedly connected to a motor, one end of the one-way gear set is provided with a belt, one end of the belt is transmission-connected to a rotating rod, one end of the rotating rod is fixedly installed with a gear, one end of the centrifugal bucket is provided with teeth, and the gear is engaged with the teeth.

[0009] Preferably, the mixing barrel is provided with a feeding port, the centrifugal barrel and one side of the filter box are both provided with a feeding port, and a valve is provided on the feeding port on one side of the centrifugal barrel.

[0010] Preferably, the fixing frame is arranged inside the centrifugal barrel, one end of the water pump is fixedly mounted on the fixing frame, and one side of the water pump is fixedly connected to a transmission pipe.

[0011] Preferably, mercury is provided inside the scale tube, and scale values ​​are provided on the scale tube. A fixed plate is installed inside the first multi-way tube, and holes are provided on the fixed plate. A second spring is fixedly connected to the fixed plate, and one end of the first push rod and the second spring are both slidably connected to a blocking ball.

[0012] Preferably, one side of the gas collecting box is fixedly connected to a second multi-way tube, a second push rod is provided inside the second multi-way tube, one end of the second push rod is fixedly connected to a bevel block, one side of the bevel block is slidably connected to the gel filtration screen, a second spring is provided on the second push rod, one end of each second push rod is connected to a blocking ball, one end of the second multi-way tube main trunk is provided with a branch, and the main trunk and branches of the second multi-way tube are fixedly connected to the filter box.

[0013] Preferably, two groups of third springs are fixedly connected inside the filter box, and one end of the two groups of third springs is fixedly connected to one side of the gel filtration screen.

[0014] Preferably, a second spring is also fixedly installed on the first push rod, one end of the second spring is fixedly connected to the scale tube, one end of the second spring is fixedly installed on one end of the first push rod, one end of the transmission tube is fixedly connected to the filter box, and a discharge port is provided on one side of the filter box.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. During centrifugation of the mixed solution, the centrifuge bucket needs to be maintained at approximately 4°C to ensure accurate and stable centrifugation of the mixed solution. Construction personnel connect an external air conditioner through the first spiral tube inlet. Cold air flows through the first spiral tube around the outside of the centrifuge bucket and is then discharged through the regulating pipe and the first multi-way pipe. The centrifuge bucket absorbs the cold air to cool down and maintain the temperature within a certain range, thereby preventing the centrifugation temperature from being too high, which may cause the phage particles to disintegrate and affect the antibody display and screening results.

[0017] 2. In the present invention, when the indoor temperature exceeds 25 degrees Celsius in the summer, the mercury in the graduated tube in the regulating pipe expands due to the rising temperature, pushing the first push rod to extend, causing the blocking ball to leave the regulating pipe opening, and some cold air to enter the second spiral tube and be discharged. The mixing barrel absorbs the cold air and cools down to 20 to 25 degrees Celsius, preventing the high temperature from changing the chemical properties of the elution solution, affecting its interaction with the antibody-antigen complex, and reducing the elution efficiency.

[0018] 3. In the present invention, when the supernatant is transferred and accumulated on the gel filtration screen, it presses the third spring downward, and the third spring rebounds to drive the blocking ball to move, opening the second multi-way pipe passage, and the cold air from the air collecting box enters the filter box. The striking frame rotates to strike the gel filtration screen, causing it to vibrate up and down under the cooperation of the striking frame and the third spring, thereby preventing supernatant accumulation and ensuring accurate filtration of antibodies. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2 This is the second schematic diagram of the overall structure of the present invention;

[0021] Figure 3 It is a cross-sectional view of the overall structure of the present invention;

[0022] Figure 4 Schematic diagram of the screening assembly structure of the present invention;

[0023] Figure 5 This is a schematic diagram of the auxiliary component structure of the present invention;

[0024] Figure 6 This is the second schematic diagram of the auxiliary component structure of the present invention;

[0025] Figure 7 This is the third schematic diagram of the auxiliary component structure of the present invention;

[0026] Figure 8 For the present invention Figure 7 A magnified view of the mechanism at point B;

[0027] Figure 9 This is the fourth schematic diagram of the auxiliary component structure of the present invention;

[0028] Figure 10 For the present invention Figure 9 Enlarged view of the A mechanism.

[0029] In the figure: 1. Equipment housing; 2. Screening component; 201. Mixing barrel; 202. Stirring rod; 203. Block; 204. First spring; 205. One-way gear set; 206. Motor; 207. Belt; 208. Rotating rod; 209. Gear; 210. Centrifugal barrel; 211. Teeth; 212. Fixing frame; 213. Water pump; 214. Transmission pipe; 3. Auxiliary component; 301. First spiral tube; 302. Adjusting pipe; 303. Scale tube; 304. First push rod; 305. Blocking ball; 306. Second spiral tube; 307. First multi-way tube; 309. Gas collecting box; 310. Second multi-way tube; 311. Second push rod; 312. Oblique block; 313. Second spring; 314. Gel filter; 315. Filter box; 316. Third spring; 317. Beating frame. DETAILED DESCRIPTION

[0030] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] See Figures 1 to 4 As shown, the present invention provides a precise antibody screening device for a phage antibody library, comprising a device housing 1, a screening assembly 2 installed inside the device housing 1, the screening assembly 2 including a mixing barrel 201, a stirring rod 202 rotatably installed inside the mixing barrel 201, one end of the stirring rod 202 is connected to a blocking block 203, the other end of the stirring rod 202 is installed with a one-way gear set 205, one end of the one-way gear set 205 is connected to a rotating rod 208, a centrifugal barrel 210 is transmission-mounted at the lower end of the rotating rod 208, and a water pump 213 is fixedly installed inside the centrifugal barrel 210;

[0032] The construction personnel pour the phage antibody library and the elution solution into the mixing barrel 201 through the feed port, and then start the motor 206. The motor 206 rotates forward, and the motor 206 drives the one-way gear set 205 to rotate forward. When the one-way gear set 205 rotates forward, the two sets of gears inside the one-way gear set 205 are in a meshing state, so that the motor 206 drives the stirring rod 202 to rotate through the one-way gear set 205. The stirring rod 202 fully mixes the phage antibody library and the elution solution.

[0033] After the phage antibody library and the elution solution are fully mixed, the construction personnel start the motor 206 to reverse. Since the two sets of gears inside the one-way gear set 205 are in meshing state when the one-way gear set 205 rotates forward, the two sets of one-way gears inside the one-way gear set 205 are not meshing with each other when the one-way gear at the upper end of the one-way gear set 205 rotates, and the one-way gear at the lower end is continuously squeezed, so that the one-way gear at the lower end of the one-way gear set 205 drives the stirring rod 202 to squeeze the block 203 downward, and the block 203 squeezes the first spring 204, and the first spring 204 undergoes elastic deformation and shrinkage, causing the block 203 to 03 leaves the discharge port of the mixing barrel 201 and the centrifugal barrel 210, allowing the mixed solution in the mixing barrel 201 to enter the centrifugal barrel 210. At the same time, the motor 206 starts to reverse, and the motor 206 drives the rotating rod 208 to rotate through the belt 207. The rotating rod 208 drives the gear 209 to rotate. Since the gear 209 is engaged with the teeth 211 at the lower end of the centrifugal barrel 210, the gear 209 drives the centrifugal barrel 210 to rotate, so that the mixed solution in the centrifugal barrel 210 is centrifuged. After the separation is completed, the mixed solution is separated into layers, and the upper layer of the centrifugal barrel 210 is the supernatant, and the lower layer is the sediment.

[0034] See Figures 5 to 10 As shown, an auxiliary component 3 is installed on the equipment housing 1, and the auxiliary component 3 includes a first spiral tube 301, which is fixedly installed on the outside of the centrifugal bucket 210, and a regulating pipe 302 is fixedly connected to one side of the first spiral tube 301. A scale tube 303 is fixedly installed inside the regulating pipe 302, and a first push rod 304 is slidably installed inside the scale tube 303. A second spiral tube 306 is fixedly connected to one side of the regulating pipe 302, and the second spiral tube 306 is fixedly installed on the outside of the mixing barrel 201. A first multi-way tube 307 is fixedly connected to one side of the regulating pipe 302, and an air collecting box 309 is fixedly connected to one side of the first multi-way tube 307. A filter box 315 is fixedly connected to one side of the air collecting box 309, and a filter box 315 is fixedly connected to one side of the filter box 315. A gel filter screen 314 is slidably installed inside the filter box 315, and a striking frame 317 is rotatably installed inside the filter box 315.

[0035] Since the centrifuge bucket 210 maintains a certain temperature range inside the centrifuge bucket 210 during centrifugation, the centrifuge effect of the mixed solution can be better guaranteed to be more accurate and stable. Therefore, the construction personnel connect an external air conditioner through the access port of the first spiral tube 301, so that the cold air passes through the first spiral tube 301 and surrounds the outside of the centrifuge bucket 210. The cold air is transmitted to the regulating pipe 302 through the first spiral tube 301, and then transmitted to the first multi-way pipe 307 through the regulating pipe 302. The first multi-way pipe 307 is transmitted to the outside. During the transportation of the cold air in the first spiral tube 301, the temperature of the cold air is absorbed by the inside of the centrifuge bucket 210, which reduces the temperature inside the centrifuge bucket 210 and keeps the temperature inside the centrifuge bucket 210 within a certain range. If the centrifugal temperature is too high, the phage particles may disintegrate, resulting in the antibodies in the phage antibody library being unable to be normally displayed on the phage surface, thereby affecting the screening results.

[0036] When the mixing barrel 201 is mixing the phage antibody library and the elution solution, the temperature of the mixing barrel 201 must be maintained between 20 and 25 degrees in the room to better ensure the mixing effect of the phage antibody library and the elution solution. If the indoor temperature exceeds 25 degrees in summer, the scale tube 303 inside the regulating pipe 302 will expand as the temperature rises. The expansion of mercury pushes the first push rod 304 inside the scale tube 303 to extend. The first push rod 304 pushes the blocking ball 305, so that the blocking ball 305 leaves the opening of the regulating pipe 302, and the opening of the regulating pipe 302 is opened, so that When the cold air conditioning pipe 302 transmits the cold air to the first multi-way pipe 307, a portion of the cold air will be transmitted to the inside of the second spiral tube 306, transported in the second spiral tube 306, and then transmitted to the outside through the transmission port of the second spiral tube 306. During the transportation process in the second spiral tube 306, the mixing barrel 201 absorbs the cold air transported inside the second spiral tube 306, so that the internal temperature of the mixing barrel 201 is maintained within a certain range, for example, between 20 degrees and 25 degrees, to prevent the internal temperature of the mixing barrel 201 from being too high in summer, which would cause the high temperature to change the chemical properties of the elution solution, affect its interaction with the antibody-antigen complex, and thus reduce the elution efficiency;

[0037] The expansion of mercury pushes the first push rod 304 inside the graduated tube 303 to extend, and at the same time, compresses the second spring 313 on the first push rod 304, causing the second spring 313 to elastically deform and contract. When the temperature of the mixing barrel 201 is between 20 and 25 degrees Celsius, the mercury recovers, and the second spring 313 is no longer under pressure. The second spring 313 recovers, and the second spring 313 drives the blocking ball 305 to reset through the first push rod 304, re-blocking the opening of the regulating pipe 302, preventing the mixing barrel 201 from continuously absorbing cold air. Too low a temperature will reduce the diffusion rate and reaction rate of molecules, slowing down the interaction between the components in the elution solution and the antibody-phage complex, thereby reducing the elution rate. The state of the plugging ball 305 blocking the opening of the regulating pipe 302 indicates that the mercury is between 20 and 25 degrees on the scale of the graduated tube 303, which also indirectly reflects that the temperature range of the mixing barrel 201 at this time is between 20 and 25 degrees. The regulating pipe 302 is made of transparent glass, which makes it easier for construction personnel to clearly see the scale value on the graduated tube 303 and adjust the temperature of the mixing barrel 201.

[0038] While the cold air is transmitted to the outside through the first manifold 307, a portion of the cold air is transmitted to the inside of the pipe on one side of the first manifold 307. The cold air squeezes the blocking ball 305 inside the pipe on one side of the first manifold 307. The blocking ball 305 is subjected to air pressure, and the blocking ball 305 drives the second spring 313 to stretch and move, thereby opening the passage inside the pipe on the one side of the first manifold 307. The cold air is then transmitted to the inside of the air collecting box 309 for collection.

[0039] When the transmission of cold air stops, the blocking ball 305 is not affected by the cold air pressure, and the second spring 313 elastically deforms and rebounds, driving the blocking ball 305 to return to its original position, re-blocking the passage inside the pipe on one side of the first multi-way pipe 307, thereby preventing the cold air collected in the air collecting box 309 from leaking out;

[0040] After the mixed solution is centrifuged inside the centrifuge bucket 210, the construction personnel start the water pump 213 to extract the supernatant inside the centrifuge bucket 210. The supernatant is transferred to the inside of the filter box 315 through the transfer pipe 214. The supernatant is then filtered through the gel filtration screen 314. The filtered supernatant is directly transferred out through the discharge port on one side of the filter box 315. The construction personnel collect it and filter the supernatant again to make the antibody content in the supernatant more accurate. After the supernatant is completely extracted by the water pump 213, the construction personnel open the valve on the discharge port, and the sediment is finally discharged through the feed port below the mixing bucket 201.

[0041] When the gel filtration screen 314 is in the initial position, the gel filtration screen 314 squeezes the inclined block 312, and the inclined block 312 pushes the blocking ball 305 through the second push rod 311, so that the blocking ball 305 is blocked at the opening of the second multi-way tube 310. When the inclined block 312 pushes the blocking ball 305 through the second push rod 311, it squeezes the second spring 313 on the second push rod 311. When the supernatant is transmitted through the transmission tube 214, the position of the supernatant on the gel filtration screen 314 remains unchanged, which may cause the supernatant to accumulate on the gel filtration screen 314. At this time, the supernatant accumulates on the gel filtration screen 314, which increases the gravity of the gel filtration screen 314. The gel filtration screen 314 presses down the third spring 316, causing the third spring 316 to elastically deform and contract, thereby the gel filtration The screen 314 moves downward, and the inclined block 312 and the third spring 316 are not squeezed by the gel filtration screen 314. The third spring 316 undergoes elastic deformation and rebounds to reset, so that the third spring 316 drives the blocking ball 305 to move through the second push rod 311, opening the passage of the second multi-way pipe 310, and the cold air collected inside the air collecting box 309 is transmitted to the inside of the filter box 315 through the passage on the other side of the second multi-way pipe 310. The transmitted cold air drives the striking frame 317 to rotate, and the striking frame 317 will strike the gel filtration screen 314, causing the gel filtration screen 314 to rise. Under the cooperation of the striking frame 317 and the third spring 316, the gel filtration screen 314 vibrates up and down to prevent the supernatant from accumulating on the gel filtration screen 314 and affecting the accurate filtration of the antibodies inside the supernatant.

[0042] In an optional embodiment, one end of the blocking block 203 is fixedly connected to a first spring 204 , a telescopic sleeve is provided on the outside of the first spring 204 , and one end of the first spring 204 is fixedly connected to a fixing bracket 212 , which is fixedly installed on the device housing 1 .

[0043] It should be noted that the telescopic sleeve is used to protect the first spring 204 inside, and the fixing frame 212 is used to fix the first spring 204 .

[0044] In an optional embodiment, one end of the one-way gear set 205 is fixedly connected to the motor 206, one end of the one-way gear set 205 is provided with a belt 207, one end of the belt 207 is transmission-connected to the rotating rod 208, one end of the rotating rod 208 is fixedly installed with a gear 209, one end of the centrifugal barrel 210 is provided with teeth 211, and the gear 209 is engaged with the teeth 211.

[0045] It should be noted that when the motor 206 is reversed, the motor 206 will drive the rotating rod 208 to rotate through the belt 207, and the rotating rod 208 will drive the gear 209 to rotate. Since the gear 209 is engaged with the teeth 211 at the lower end of the centrifuge bucket 210, the gear 209 drives the centrifuge bucket 210 to rotate, so that the mixed solution inside the centrifuge bucket 210 is centrifuged.

[0046] In an optional embodiment, a feeding port is provided on the mixing barrel 201 , a feeding port is provided on one side of the centrifugal barrel 210 and the filter box 315 , and a valve is provided on the feeding port on one side of the centrifugal barrel 210 .

[0047] It should be noted that the construction personnel open the valve on the discharge port, and the sediment is finally discharged through the feed port below the mixing barrel 201. After the supernatant is completely extracted by the water pump 213, the construction personnel open the valve on the discharge port, and the sediment is finally discharged through the feed port below the mixing barrel 201.

[0048] In an optional embodiment, the fixing frame 212 is disposed inside the centrifugal barrel 210 , one end of the water pump 213 is fixedly mounted on the fixing frame 212 , and one side of the water pump 213 is fixedly connected to a transmission pipe 214 .

[0049] It should be noted that the construction personnel start the water pump 213 to extract the supernatant from the centrifuge barrel 210 , and the supernatant is transferred to the filter box 315 through the transfer pipe 214 , so that the supernatant is filtered by the gel filter sieve 314 .

[0050] In an optional embodiment, mercury is provided inside the calibrated tube 303, and scale values ​​are provided on the calibrated tube 303. A fixed plate is installed inside the first multi-way tube 307, and a hole is provided on the fixed plate. A second spring 313 is fixedly connected to the fixed plate, and one end of the first push rod 304 and the second spring 313 are both slidably connected to a blocking ball 305.

[0051] It should be noted that as the temperature of the calibrated tube 303 inside the regulating pipe 302 rises, the mercury inside the calibrated tube 303 expands, and the expansion of mercury pushes the first push rod 304 inside the calibrated tube 303 to extend, and the cold air squeezes the blocking ball 305 in the pipe on one side of the first multi-way pipe 307. The blocking ball 305 is stretched and moved by the air pressure to drive the second spring 313 to open the pipe passage on this side, and the cold air is transmitted to the gas collecting box 309 for collection; when the cold air stops being transmitted, the blocking ball 305 is not affected by the air pressure, and the elastic rebound of the second spring 313 drives the blocking ball 305 to reset, re-blocking the passage to prevent the cold air in the gas collecting box 309 from leaking out.

[0052] In an optional embodiment, a second multi-way tube 310 is fixedly connected to one side of the air collecting box 309, a second push rod 311 is provided inside the second multi-way tube 310, one end of the second push rod 311 is fixedly connected to an inclined block 312, one side of the inclined block 312 is slidably connected to the gel filtration screen 314, a second spring 313 is provided on the second push rod 311, one end of the second push rod 311 is connected to a blocking ball 305, a branch is provided at one end of the main trunk of the second multi-way tube 310, and the main trunk and branches of the second multi-way tube 310 are fixedly connected to the filter box 315.

[0053] It should be noted that the inclined block 312 pushes the blocking ball 305 to block the opening of the second multi-channel tube 310 through the second push rod 311. During this process, the inclined block 312 will squeeze the second spring 313 on the second push rod 311, and the third spring 316 drives the blocking ball 305 to move through the second push rod 311, opening the passage of the second multi-channel tube 310, and the cold air in the air collecting box 309 is transmitted to the filter box 315 through its other passage.

[0054] In an optional embodiment, two groups of third springs 316 are fixedly connected inside the filter box 315 , and one end of the two groups of third springs 316 is fixedly connected to one side of the gel filtration screen 314 .

[0055] It should be noted that the cooperation between the striking frame 317 and the third spring 316 causes the gel filter screen 314 to vibrate up and down.

[0056] In an optional embodiment, a second spring 313 is also fixedly mounted on the first push rod 304, one end of the second spring 313 is fixedly connected to the calibrated tube 303, one end of the second spring 313 is fixedly mounted on one end of the first push rod 304, and one end of the transmission tube 214 is fixedly connected to the filter box 315.

[0057] It should be noted that when the mercury is restored, the second spring 313 drives the blocking ball 305 to reset through the first push rod 304, and re-blocks the opening of the regulating pipe 302 to prevent the mixing barrel 201 from continuously absorbing cold air.

[0058] Working principle: The construction personnel pour the phage antibody library and elution solution into the mixing barrel 201 through the feed port, start the motor 206 to rotate forward, and drive the stirring rod 202 to rotate through the one-way gear set 205 to fully mix the two;

[0059] After mixing is complete, motor 206 reverses, disengaging one-way gear set 205. The lower one-way gear drives stirring rod 202 downward to squeeze block 203, forcing it out of the discharge port and allowing the mixed solution to enter centrifuge bucket 210. Simultaneously, motor 206 drives rotating rod 208 and gear 209 via belt 207, further rotating centrifuge bucket 210 for centrifugal separation, separating the solution into a supernatant and a precipitate.

[0060] During centrifugation of the mixed solution, the centrifuge bucket 210 must be maintained within a certain temperature range to ensure accurate and stable centrifugation of the mixed solution. The operator connects an external air conditioner through the first spiral tube 301. The cold air flows through the first spiral tube 301 and surrounds the outside of the centrifuge bucket 210. The cold air is then discharged through the regulating pipe 302 and the first multi-way pipe 307. The centrifuge bucket 210 absorbs the cold air and cools down, maintaining the temperature within a certain range. This prevents the centrifugation temperature from being too high, which may cause the phage particles to disintegrate and affect the antibody display and screening results.

[0061] When mixing the phage antibody library and the elution solution, the temperature of the mixing barrel 201 must be maintained between 20 and 25 degrees Celsius. When the indoor temperature exceeds 25 degrees Celsius in summer, the mercury in the graduated tube 303 in the regulating pipe 302 expands due to the temperature rise, pushing the first push rod 304 to extend, causing the blocking ball 305 to leave the opening of the regulating pipe 302. Some cold air enters the second spiral tube 306 and is discharged. The mixing barrel 201 absorbs the cold air and cools down to a range of 20 to 25 degrees Celsius. This prevents the high temperature from changing the chemical properties of the elution solution, affecting its interaction with the antibody-antigen complex, and reducing the elution efficiency.

[0062] When the cold air is transmitted through the first multi-way pipe 307, part of it enters the air collecting box 309 for collection. Initially, the gel filter screen 314 squeezes the inclined block 312, causing the blocking ball 305 to block the opening of the second multi-way pipe 310. When the supernatant is transmitted and accumulated on the gel filter screen 314, it presses the third spring 316 downward, and the third spring 316 rebounds to drive the blocking ball 305 to move, opening the passage of the second multi-way pipe 310. The cold air from the air collecting box 309 enters the filter box 315, and the striking frame 317 rotates to strike the gel filter screen 314, causing it to vibrate up and down under the cooperation of the striking frame 317 and the third spring 316, preventing supernatant accumulation and ensuring accurate filtration of antibodies.

[0063] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A precise antibody screening device for a phage antibody library, comprising a device housing (1), characterized in that: A screening assembly (2) is installed inside the device housing (1), and the screening assembly (2) includes a mixing barrel (201), a stirring rod (202) is rotatably installed inside the mixing barrel (201), one end of the stirring rod (202) is connected to a blocking block (203), the other end of the stirring rod (202) is installed with a one-way gear set (205), one end of the one-way gear set (205) is connected to a rotating rod (208), a centrifugal barrel (210) is rotatably installed at the lower end of the rotating rod (208), and a water pump (213) is fixedly installed inside the centrifugal barrel (210); An auxiliary component (3) is installed on the device housing (1), and the auxiliary component (3) includes a first spiral tube (301), the first spiral tube (301) is fixedly installed on the outside of the centrifugal bucket (210), one side of the first spiral tube (301) is fixedly connected to a regulating pipe (302), a scale tube (303) is fixedly installed inside the regulating pipe (302), a first push rod (304) is slidably installed inside the scale tube (303), one side of the regulating pipe (302) is fixedly connected to a second spiral tube (306), and the first push rod (306) is fixedly installed inside the regulating pipe (302). Two spiral tubes (306) are fixedly installed on the outside of the mixing barrel (201); one side of the regulating pipe (302) is fixedly connected to a first multi-way pipe (307); one side of the first multi-way pipe (307) is fixedly connected to an air collecting box (309); one side of the air collecting box (309) is fixedly connected to a filter box (315); one side of the filter box (315) is fixedly connected to a water pump (213); a gel filter screen (314) is slidably installed inside the filter box (315); and a striking frame (317) is rotatably installed inside the filter box (315).

2. The precise antibody screening device for phage antibody library according to claim 1, characterized in that: One end of the blocking block (203) is fixedly connected to a first spring (204), a telescopic sleeve is provided on the outside of the first spring (204), and one end of the first spring (204) is fixedly connected to a fixing frame (212), and the fixing frame (212) is fixedly mounted on the device housing (1).

3. The precise antibody screening device for phage antibody library according to claim 1, characterized in that: One end of the one-way gear set (205) is fixedly connected to a motor (206), one end of the one-way gear set (205) is provided with a belt (207), one end of the belt (207) is transmission-connected to a rotating rod (208), one end of the rotating rod (208) is fixedly mounted with a gear (209), one end of the centrifugal bucket (210) is provided with teeth (211), and the gear (209) is meshed with the teeth (211).

4. The precise antibody screening device for phage antibody library according to claim 1, characterized in that: The mixing barrel (201) is provided with a feeding port, and one side of the centrifugal barrel (210) and the filter box (315) are both provided with a feeding port, and a valve is provided on the feeding port on one side of the centrifugal barrel (210).

5. The precise antibody screening device for phage antibody library according to claim 2, characterized in that: The fixing frame (212) is arranged inside the centrifugal barrel (210), one end of the water pump (213) is fixedly mounted on the fixing frame (212), and one side of the water pump (213) is fixedly connected to a transmission pipe (214).

6. The precise antibody screening device for phage antibody library according to claim 1, characterized in that: Mercury is provided inside the graduated tube (303), and graduated values ​​are provided on the graduated tube (303). A fixed plate is installed inside the first multi-way tube (307), and a hole is provided on the fixed plate. A second spring (313) is fixedly connected to the fixed plate. One end of each of the first push rod (304) and the second spring (313) is slidably connected to a blocking ball (305).

7. The precise antibody screening device for phage antibody library according to claim 1, characterized in that: One side of the gas collecting box (309) is fixedly connected to a second multi-way tube (310), a second push rod (311) is provided inside the second multi-way tube (310), one end of the second push rod (311) is fixedly connected to an inclined block (312), one side of the inclined block (312) is slidably connected to the gel filtration screen (314), a second spring (313) is provided on the second push rod (311), one end of each of the second push rods (311) is connected to a blocking ball (305), a branch is provided at one end of the trunk of the second multi-way tube (310), and the trunk and branches of the second multi-way tube (310) are fixedly connected to the filter box (315).

8. The precise antibody screening device for phage antibody library according to claim 1, characterized in that: Two groups of third springs (316) are fixedly connected inside the filter box (315), and one end of the two groups of third springs (316) is fixedly connected to one side of the gel filtration screen (314).

9. The precise antibody screening device for phage antibody library according to claim 5, characterized in that: A second spring (313) is also fixedly mounted on the first push rod (304), one end of the second spring (313) is fixedly connected to the scale tube (303), one end of the second spring (313) is fixedly mounted on one end of the first push rod (304), and one end of the transmission tube (214) is fixedly connected to the filter box (315).

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