A composition having multi-effect anti-aging efficacy and applications thereof

Abstract

The present application relates to the daily chemical technology field, especially to a composition with multiple anti-aging effects and application thereof.The composition comprises retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soybean milk fermentation product filtrate and resveratrol.The present application is a composition with a mild triple anti-aging system for promoting collagen regeneration and a preparation method thereof, which contains retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soybean milk fermentation product filtrate and resveratrol, has obvious effects on skin epidermis and dermis aging, up-regulates skin flora balance, enhances the water-locking ability of the stratum corneum, improves the penetration rate of active ingredients, and increases cell vitality on the epidermis;in the epidermis+dermis, it has antioxidant and anti-inflammatory properties, the components are mild and have good absorption effect, and there is no need to establish tolerance, so that the skin resistance is consolidated, and it is especially suitable for sensitive skin.

Description

Technical Field

[0001] This invention relates to the field of daily chemical technology, and in particular to a composition with multiple anti-aging effects and its application. Background Technology

[0002] Skin aging refers to the degeneration of cell structure and function in skin tissue under the continuous influence of internal and external environments. It typically manifests as dry skin, pigmentation spots, reduced skin elasticity, sagging, roughness, and wrinkles. In summary, skin aging can be divided into endogenous aging (accounting for 20%) and exogenous aging (accounting for 80%). Endogenous aging refers to aging caused by uncontrollable physiological factors such as genetics and endocrine disorders, while exogenous aging is caused by environmental factors, such as light radiation, environmental pollution, and unhealthy lifestyle habits. Besides sun exposure, oxidative stress, non-enzymatic glycation, and genetic and environmental factors, the most significant causes of skin aging are a decrease in the number and function of fibroblasts, rapid collagen loss, and reduced synthesis of mucopolysaccharides. This leads to a loosening of the dermal reticular structure, forming depressions, and resulting in wrinkles, sagging, dryness, and roughness on the skin surface. Elastin allows the skin to stretch and fold, maintaining and supporting its elasticity, and determining its suppleness and softness. Collagen is the main component of skin, accounting for 72% of its composition, with 80% of that in the dermis. Therefore, collagen plays a vital role in plumping the skin, rejuvenating it, and improving its fullness. Data shows that 53.2% of consumers begin focusing on anti-aging between the ages of 26 and 35, and 23.2% between 18 and 25. With the arrival of China's aging society, "anti-aging" has become a highly discussed topic. People's lifestyles and makeup habits are changing, and everyone wants to maintain a youthful appearance and minimize signs of aging, directly contributing to the rapid growth in sales of anti-aging products. The accelerating aging process provides enormous development potential for my country's anti-aging market.

[0003] One of the core functions of anti-aging products is to repair damaged cells and the skin barrier function. Single ingredients or low concentrations can only address a few individual signs of aging. A single product cannot meet the complex needs of anti-aging. Layering products can potentially increase the burden on the skin, exceed tolerance limits, and result in short-term rebound effects upon discontinuation. These issues constitute unresolved problems for anti-aging users. Furthermore, according to research, 42.2% of anti-aging users reported not finding an anti-aging product suitable for their skin type or specific skin problems. Moreover, many products are dominated by various emulsifiers or preservatives, which can easily disrupt the skin's ecological balance and lead to other skin problems with long-term use. Starting with the crucial step of activating cells, many brands neglect this crucial step. When skin lacks vitality and has poor metabolic absorption, simply supplementing with collagen lacks a comprehensive solution for multiple skin problems. Not only is it ineffective, but poor absorption can also clog pores and accelerate aging. Summary of the Invention

[0004] To address the shortcomings of the aforementioned technologies, this invention studies the entire aging process. Consequently, anti-aging concepts among consumers are undergoing a profound transformation—anti-aging ingredients face dual demands: a need for multi-dimensional improvements through complex efficacy, and an urgent desire to establish a sustainable, safe, and gentle anti-aging model. This is driving the consumer market to shift from a focus on "efficacy above all else" to a value proposition that balances efficacy and safety. The composition of this invention provides a comprehensive and systematic solution to anti-aging from various aspects, such as activating cells, replenishing collagen, and repairing and soothing the skin.

[0005] In this composition, retinol in the epidermis and dermis stimulates collagen regeneration, rapidly improving wrinkles; in the dermis, palmitoyl tripeptide-1 continuously activates collagen synthesis, palmitoyl tetrapeptide-7 prevents collagen degradation through anti-inflammatory effects, resveratrol neutralizes free radicals, and synergistically with retinol to reduce photosensitivity, inhibit MMP enzyme damage to collagen, and prolong the duration of activity of other components; natural lactic acid in the fermentation products gently metabolizes keratin, reducing retinol irritation; peptide components buffer the risk of peeling from retinol; and the oxidation system protects... It protects newly formed collagen from oxidative damage; forming a triple anti-aging closed loop of immediate repair, long-lasting regeneration, and prevention of loss. It regulates the skin's microecology balance with lactobacillus / soy milk ferment filtrate, enhances the stratum corneum's water-locking ability, and improves the penetration rate of active ingredients. Ultimately, it constructs a new paradigm of composite anti-aging, integrating "antioxidant-anti-inflammatory-collagen-promoting," particularly suitable for the comprehensive care needs of early signs of aging, photo-aged skin, and skin damaged by lack of sleep. It provides users with a fundamental, gentle, safe, and long-lasting anti-aging solution. It surpasses traditional ingredients in terms of broad-spectrum antioxidant activity, collagen regeneration efficiency, and anti-inflammatory depth, truly ushering in an era of gentle, long-lasting, precise, and highly effective anti-aging skincare.

[0006] To solve the above-mentioned technical problems, the present invention provides a composition with multiple anti-aging effects, the composition comprising: retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol.

[0007] In one embodiment of the present invention, the retinol is encapsulated and modified retinol, and the resveratrol is liposome-derived resveratrol.

[0008] In one embodiment of the present invention, the mass ratio of retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soybean fermentation product filtrate, and resveratrol is (0.01-0.25): (0.0003-0.0008): (0.00015-0.0004): (0.05-0.2): (0.0001-0.03).

[0009] In one embodiment of the present invention, the mass ratio of retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol is 0.2:0.0007:0.0004:0.2:0.003.

[0010] As one embodiment of the present invention, the method for preparing the encapsulated modified retinol is as follows:

[0011] Retinol was dissolved in a mixed solvent of ethanol / propylene glycol at a concentration of 3 mg / mL; the volume ratio of ethanol to propylene glycol was 3:2.

[0012] Weigh out 10 molar amounts of β-cyclodextrin relative to retinol, and add 0.7 molar amounts of stigmasterol relative to retinol. Add deionized water at 45–55°C to form a suspension and stir until homogeneous.

[0013] The organic phase containing retinol was slowly added dropwise to the suspension; the reaction was continued for 4 hours under magnetic stirring, and the temperature was controlled below 50°C in the dark. Subsequently, the mixture was freeze-dried to obtain the inclusion compound powder, which was then used to encapsulate and modify the retinol.

[0014] As one embodiment of the present invention, the method for preparing resveratrol after liposome implantation is as follows:

[0015] S01 Weigh 2g of lecithin, 0.8g of cholesterol, 0.1g of ceramide NP and 0.3g of phosphatidylserine, and place them in a glass beaker; add 10mL of anhydrous ethanol until completely dissolved;

[0016] S02 Add 0.1g of resveratrol to the mixture obtained in step S01, and continue stirring in a 60℃ water bath to form a uniform and transparent lipid phase;

[0017] S03: Mix glycerol and deionized water at a mass ratio of 2:96 and maintain the temperature at 55-60℃. Then, slowly add the transparent lipid phase dropwise to the aqueous phase while stirring at 800 rpm to maintain uniform emulsification. After the addition is complete, continue stirring for 15-20 minutes.

[0018] As one embodiment of the present invention, the composition having multiple anti-aging effects is applied in the daily chemical industry.

[0019] An essence, characterized in that its preparation ingredients include the aforementioned composition with multiple anti-aging effects.

[0020] In one embodiment of the present invention, the raw materials for preparing the essence include: water, pentylene glycol, hexanediol, glycerin, allantoin, disodium EDTA, trehalose, xanthan gum, sodium polyglutamate, β-glucan, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, squalane, tocopheryl acetate, caprylic / capric triglyceride, polyacrylate-13 polyglycerol-10 stearate, C14-22 alcohol / C12-20 alkyl glucoside, phosphatidylcholine, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, retinol, resveratrol, and Lactobacillus / soybean fermentation product filtrate.

[0021] By adopting the above technical solution, the present invention has the following beneficial effects:

[0022] Retinol: Utilizing a novel microencapsulation technology, this product offers a high concentration of retinol, resulting in a more stable and gentler sustained-release formulation. Retinol increases gene expression of elastin and fibrin-1 in normal human dermal fibroblasts (NHDF), 3D dermal culture models, and human skin explants. It is one of the most potent collagen protectors and promoters, promoting elastin synthesis, procollagen synthesis and elastic fiber network formation, type I and type III collagen synthesis, and increasing gene expression of elastin / fibrin-1 / type I collagen. Furthermore, it increases hyaluronic acid synthesis and effectively combats the activity of MMPs, thus preventing signs of photoaging, increasing skin firmness, plumpness, and smoothness, increasing epidermal thickness, and slowing down the aging symptoms caused by the loss of these two complexes, thereby preventing fine lines and wrinkles before they form. Retinol is a gentle exfoliant that regulates the shedding process of keratinocytes in the stratum corneum. It also softens the stratum corneum and moisturizes the skin, thus improving skin radiance and hydration. Retinol promotes cell proliferation and stimulates cell renewal, thereby thickening the epidermis and repairing and improving damage in naturally aging and photo-aged skin. Since the healing of skin wounds requires the migration and proliferation of keratinocytes, retinol can promote skin repair and regeneration.

[0023] Palmitoyl tripeptide-1 plays an important feedback regulatory role in connective tissue remodeling and cell proliferation. It can enhance fibroblast activity and promote the production of abundant skin repair proteins during skin repair. It also has effects such as promoting collagen and mucopolysaccharide synthesis, moisturizing, firming skin, and reducing wrinkles.

[0024] Palmitoyl Tetrapeptide-7: During aging, there is a strong correlation between the decrease in DHEA and the increase in IL-6. DHEA controls the levels of inflammatory cytokines in the circulatory system, while palmitoyl tetrapeptide-7 regulates the inflammatory factor (IL-6), mimics DHEA (youth hormone), and maintains skin immune homeostasis, thereby reducing the damage of various inflammations to skin tissues. It inhibits the release of the inflammatory factor IL-6 by acting on keratinocytes and fibroblasts, reducing inflammation-related skin damage, firming the skin, and diminishing wrinkles. It is hailed as a "youth hormone peptide."

[0025] Lactobacillus / Soybean Fermentation Product Filtrate: The synergistic effect of Lactobacillus / Soybean Fermentation Product Filtrate and highly nutritious black soybeans (through biomimetic fermentation) significantly regulates the skin's microbiome. In vitro tests show that this ingredient effectively enhances the diversity of beneficial bacteria in the skin, increases the abundance of Staphylococcus epidermidis, maintains a healthy, slightly acidic pH environment, reduces the risk of allergies, quickly relieves pain caused by external stimuli, improves inflammatory responses, and instantly increases skin hydration for long-lasting deep moisturization. Furthermore, it soothes and repairs the skin in multiple dimensions, helping to maintain skin stability and health, meeting consumers' evolving needs for optimal skin condition. In vitro experiments further confirm that this ingredient promotes collagen synthesis in fibroblasts, accelerates hyaluronic acid production, enhances the regenerative capacity of keratinocytes, and improves the healing and repair efficiency of damaged tissues.

[0026] Resveratrol: Resveratrol is a non-flavonoid phenolic substance, belonging to the stilbene class of phytoalexins and antioxidants. The skin-care effects of resveratrol have received increasing attention in recent years, and its mechanism of action is now largely clear. Resveratrol has significant whitening and anti-photodamage effects, enhancing protection against ultraviolet rays, boosting the body's antioxidant capacity, and reducing inflammation. Furthermore, resveratrol can improve excessive keratinization, promote keratinocyte differentiation, improve skin's repair capabilities, and reduce scar formation. Attached Figure Description

[0027] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0028] Figure 1 The graph shows the performance test results of the serum prepared in Example 2. Detailed Implementation

[0029] The present invention will be further explained below with reference to specific embodiments.

[0030] The composition of the present invention is formulated into an anti-aging serum, as shown in Table 1 below:

[0031]

[0032]

[0033]

[0034]

[0035] The preparation method of lipid resveratrol in the table above is as follows:

[0036] (1) Mix 2g of lecithin, 0.4g of stigmasterol and 0.1g of resveratrol in an ethanol:propylene glycol (4:1) system and stir at 60°C to form a transparent phase.

[0037] (2) Using pH 6.0 PBS buffer as the aqueous phase, the lipid phase was added dropwise after heating to 60°C. After high shear homogenization at 8000 rpm for 5 minutes, the lipids were extruded through a membrane to obtain liposomes with uniform particle size. Finally, freeze-dried liposome powder was obtained by freeze drying.

[0038] The preparation method of the serum is as follows, as shown in the table above:

[0039] Group A: Water, pentylene glycol, hexanediol, glycerol, disodium EDTA, trehalose, xanthan gum, sodium polyglutamate, beta-glucan, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer;

[0040] Group B: Squalane, Caprylic / Capric Triglyceride, Tocopheryl Acetate, Polyglycerol-10 Stearate, C14-22 Alcohol / C12-20 Alkyl Glucoside, Phosphatidylcholine;

[0041] Group C: Palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, retinol, resveratrol, Lactobacillus / soy milk fermentation product filtrate, allantoin;

[0042] S01 A phase: Aqueous phase preparation: Take purified water, heat to 75±2℃, add glycerol, pentanediol, hexanediol, trehalose, and disodium EDTA in sequence, and stir until completely dissolved; disperse xanthan gum, which can be pre-wetted with a small amount of glycerol before addition, and stir until no flocculation occurs; add sodium polyglutamate, β-glucan, and hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer in sequence, stir evenly, and maintain the temperature at 75℃ for later use;

[0043] S02 B phase: Oil phase heating and dissolution: Squalane, triglycerides, and tocopheryl acetate are placed in an oil phase beaker and heated to 75±2℃; polyglycerol-10 stearate and C14-22 alcohol / C12-20 alkyl glucoside are added and stirred thoroughly until completely dissolved; finally, phosphatidylcholine is added and stirring is continued until a uniform and clear state is reached.

[0044] S03. Slowly add the oil phase (B) to the water phase (A) and shear emulsify for 5-10 minutes under an 8000 rpm shear emulsifier; then switch to a stirrer and maintain the temperature at about 70°C for 10 minutes to form the initial emulsion.

[0045] Slowly cool the S04 emulsion system to below 40°C, add the remaining ingredients while stirring at low speed to avoid air bubbles, and it is ready.

[0046] illustrate:

[0047] 1. Comparative Example 1 is an essence obtained by removing palmitoyl tripeptide-1 from the essence of Example 2 of the present invention.

[0048] 2. Comparative Example 2 is an essence obtained by removing palmitoyl tetrapeptide-7 from the essence of Example 2 of the present invention.

[0049] 3. Comparative Example 3 is an essence obtained by removing retinol from the essence of Example 2 of the present invention.

[0050] 4. Comparative Example 4 is an essence obtained by removing resveratrol from the essence of Example 2 of the present invention.

[0051] 5. Comparative Example 5 is the essence obtained by removing the lactobacillus / soy milk fermentation product filtrate from the essence of Example 2 of the present invention.

[0052] 6. Comparative Example 6 is an essence obtained by using commercially available unencapsulated retinol instead of the retinol in the essence of Example 2 of the present invention.

[0053] 7. Comparative Example 7 is an essence obtained by removing polyglycerol-10 stearate, C14-22 alcohol, C12-20 alkyl glucoside and phosphatidylcholine from the essence of Example 2 of the present invention.

[0054] 8. Comparative Example 8 is an essence obtained by using commercially available lipid-free resveratrol instead of the resveratrol in the essence of Example 2 of the present invention.

[0055] 9. Comparative Example 9 is an essence obtained by using resveratrol from other commercially available lipids in the essence of Example 2 of the present invention.

[0056] Performance testing

[0057] (I) Efficacy Testing

[0058] I. Testing Instruments:

[0059] 1. This invention uses the Finnish Delfin ElastiMeter (skin elasticity measuring instrument). The ElastiMeter uses a pressure head to gently press on the skin. When external force is applied to the skin, the skin resists changes in its shape. The skin's response under short-term load indicates its instantaneous elastic properties, thus yielding the skin's elasticity value.

[0060] 2. Using the Finnish Delfin Antera 3D camera, Antera3D employs innovative optical methods and sophisticated mathematical algorithms to acquire three-dimensional images. This allows data to be extracted from images related to the three-dimensional shape of the skin, enabling the quantification of treatment effects and monitoring of changes over time. For example, it measures skin texture, smoothness, and the depth and width of fine lines and wrinkles. With its multispectral analysis, Antera software can also measure pigmentation and redness, providing meaningful information about sun damage, wrinkles, and more.

[0061] 3. Using the Finnish Delfin VapoMeter SWL5201 transdermal moisture loss meter, the VapoMeter has a cylindrical chamber containing relative humidity and temperature sensors. Shortly after the instrument comes into contact with the skin, the relative humidity (RH%) inside the measurement chamber increases linearly. The SCH value is calculated based on the increase in RH%. During measurement intervals, the measurement chamber is passively ventilated. The VapoMeter uses a closed-chamber measurement principle, therefore the measurement is unaffected by indoor airflow. The short measurement time ensures minimal obstruction of normal evaporation.

[0062] II. Experimental Principle:

[0063] Transepidermal water loss (SCH) measures the change in water vapor pressure at the surface of the skin. SCH indicates the amount of water lost from the stratum corneum and is an important standard for evaluating the barrier function of the stratum corneum. A lower SCH value indicates better skin barrier function, and vice versa. By testing the SCH value, the efficacy of moisturizing and repairing cosmetics can be evaluated. It can also be used to monitor allergic patch tests, contact dermatitis, physical therapy, burns, and new tissue formation, allowing for timely detection of whether the skin's protective function has been compromised. The unit for SCH is g / hm². 3 .

[0064] III. Test Subjects:

[0065] Each group consists of 20 people, totaling 11 groups, with volunteers aged 30-65 who meet the experimental requirements.

[0066] IV. Test conditions:

[0067] Under conditions of room temperature 21±1℃ and relative humidity 50±10%, and with real-time dynamic monitoring, the subjects sat still for 30 minutes before the relevant data were tested.

[0068] V. Experimental Procedure:

[0069] This experiment was divided into 11 groups. Group 1 used Example 1 of this invention on the entire face, Group 2 used Example 3 of this invention on the entire face, Group 3 used Comparative Example 7 on the entire face, and Groups 4-11 used Example 2 of this invention on the left side of the face. Comparative Examples 1-6 and 8-9 were applied to the right side of each group. The products were applied to the face twice a day, morning and evening, for 4 weeks. The skin elasticity (ISE) value, transepidermal water loss (SCH) value, average wrinkle length (WRC) (mm), average wrinkle width (WRK) (mm), and average wrinkle depth (WRS) (mm) were recorded. Then, one area of ​​each volunteer's face, one on each side of the forehead, one corner of the eye, and one cheek, was photographed using Antera 3D and archived.

[0070] VI. Experimental Results:

[0071] 1. Skin stratum corneum moisture content (SCH value) test

[0072] In this test, the SCH measured by the subjects in week 0 was set as SCH0, and the SCH in week 4 was set as SCH4. The change value of SCH was ΔSCH, ΔSCH = SCH4 - SCH0, and the rate of change of ΔSCH = (SCH4 - SCH0) / SCH0 * 100%. The rate of change of ΔSCH was positive, and the higher the value, the more moisture the skin had after four weeks of using the product, indicating that the skin was more hydrated and plump, and the better the product was at increasing the skin's moisture content.

[0073] Table 2 shows the average rate of change of ΔSCH for each group of 20 people after 4 weeks of use, compared with the initial value, as follows:

[0074] Table 2. Change rate of skin stratum corneum water content (SCH)

[0075]

[0076]

[0077] 2. Skin elasticity (ISE) test

[0078] In this test, the ISE measured by the subjects in week 0 was set as ISE0, and the ISE in week 4 was set as ISE4. The change in ISE was ΔISE, where ΔISE = ISE4 - ISE0, and the rate of change of ΔISE = (ISE4 - ISE0) / ISE0 * 100%. The rate of change of ΔISE is positive, and the higher the value, the greater the increase in skin elasticity after four weeks of product use, indicating higher skin elasticity, firmer, fuller, and more elastic skin. Table 3 shows the average rate of change of ΔISE for each group of 20 people after 4 weeks of use compared with the initial value, as follows:

[0079] Table 3. Change rate of skin elasticity (ISE)

[0080] Group △ISE change rate (%) Example 1 24.31 Example 2 29.061 Example 3 33.53 Comparative Example 1 17.65 Comparative Example 2 16.74 Comparative Example 3 15.43 Comparative Example 4 19.86 Comparative Example 5 12.32 Comparative Example 6 10.27 Comparative Example 7 8.94 Comparative Example 8 11.85 Comparative Example 9 13.67

[0081] Skin wrinkle length (WRC value), average wrinkle width (WRK), and average wrinkle depth (WRS) test: In this test, the average wrinkle length, width, and depth measured by the subject at week 0 are set as WRC0, WRK0, and WRS0, respectively. The average wrinkle length, width, and depth measured at week 4 are set as WRC4, WRK4, and WRS4, respectively. The wrinkle changes are ΔWRC, ΔWRK, and ΔWRS, where ΔWRC = WRC4 - WRC0, ΔWRK = WRK4 - WRK0, and ΔWRS = WRK0 - WRK0. S4-WRS0; △WRC change rate = (WRC4-WRC0) / WRC0*100%, △WRK change rate = (WRK4-WRK0) / WRK0*100%, △WRS change rate = (WRS4-WRS0) / WRS0*100%; △WRC, △WRK, and △WRS change rates are negative, and the lower the value, the shorter the wrinkle length, the narrower the wrinkle width, and the shallower the wrinkle depth. This indicates a better ability of the product to delay wrinkle formation, resulting in smoother and younger-looking skin. Table 4 shows the average change rates of wrinkle length, width, and depth for each group of 20 people after 4 weeks of use compared to the initial values, as follows:

[0082] Table 4. Rate of change of wrinkle length (WRC), width (WRK), and depth (WRS)

[0083]

[0084]

[0085] (II) Safety Testing (Human Skin Patch Test)

[0086] 1. Number of participants: 40 in total, 20 males and 20 females, aged between 18 and 60.

[0087] 2. Health status: Subjects have healthy skin, no history of skin diseases or allergies, and meet the criteria for voluntary inclusion.

[0088] 3. Test method: Select a qualified patch tester and use a closed patch test method. Place about 0.020-0.2025g of the test sample into the patch tester and cover it with special adhesive tape on the back of the subject. Each subject has 11 application points. Remove the test sample after 24 hours. Observe the skin reaction at 0.5, 24 and 48 hours after removal. Score according to the skin reaction grading standard in the "Cosmetic Hygiene Standard", calculate the average value and record the results in Table 6.

[0089] Table 5 Standards for Human Skin Patch Testing

[0090]

[0091] Table 6. Skin patch test scoring results

[0092]

[0093]

[0094] Stability testing (stability testing of the composition of this invention on the final product)

[0095] 1. Experimental instruments: 101-1AB type electric heating drying oven (constant temperature 50 degrees Celsius), QC609 biological ultraviolet yellowing resistance tester

[0096] 2. Experimental procedure: The essences prepared in Examples 1 to Comparative Examples 9 were placed in 100ml containers and placed in a drying oven and a yellowing resistance test, respectively. The test results are shown in Tables 7 and 8.

[0097] Table 7 Heat Resistance Test at 50°C

[0098]

[0099] Table 8 Ultraviolet Irradiation Test

[0100]

[0101]

[0102] Before-and-after comparisons of facial wrinkle images from a subset of volunteers using embodiments of the present invention are shown in the attached image. Figure 1 As can be clearly seen from the figure, after using the essence prepared in Example 2 of the present invention on the face, the overall level of facial wrinkles is reduced, indicating that the anti-wrinkle effect of the composition of the present invention is excellent.

[0103] This invention relates to a composition and its preparation method of a triple anti-aging system with a gentle collagen-regenerating effect. The composition comprises retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, Lactobacillus / soybean fermentation product filtrate, and resveratrol. It has significant effects on both epidermal and dermal aging. In the epidermis, it regulates the skin's microbiome balance, enhances the stratum corneum's water-locking capacity, improves the penetration rate of active ingredients, and increases cell vitality. In the epidermis and dermis, it possesses antioxidant and anti-inflammatory properties. The ingredients are gentle and easily absorbed, requiring no tolerance building, thus strengthening the skin's resistance and making it particularly suitable for sensitive skin. In the dermis, it increases the production of collagen, elastin, and mucopolysaccharides, improving skin elasticity, reducing wrinkles, and achieving a comprehensive anti-aging effect. Extensive experimental evidence demonstrates the effectiveness of this composition, and each component is irreplaceable. The optimal range of addition for this composition represents the best balance between effectiveness and cost. In conclusion, this invention provides a sustained-release, gentle, triple anti-aging composition with collagen-regenerating properties.

[0104] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An essence, characterized in that, The raw materials for its preparation, by weight, include: 3 parts pentanediol, 0.5 parts hexanediol, 5 parts glycerol, 0.2 parts allantoin, 0.05 parts disodium EDTA, 1 part trehalose, 0.15 parts xanthan gum, 0.2 parts sodium polyglutamate, 0.2 parts β-glucan, 0.3 parts hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, 1.5 parts squalane, 0.5 parts tocopheryl acetate, 2 parts caprylic / capric triglyceride, 0.25 parts polyacrylate-13, 2 parts polyglycerol-10 stearate, 2 parts C14-22 alcohol / C12-20 alkyl glucoside, 0.5 parts phosphatidylcholine, 0.0005 parts palmitoyl tripeptide-1, 0.0003 parts palmitoyl tetrapeptide-7, 0.1 parts retinol, 0.002 parts resveratrol, and 0.1 parts Lactobacillus / soybean fermentation product filtrate. Water replenished to 100 servings; The retinol is prepared as follows: Retinol was dissolved in a mixed solvent of ethanol / propylene glycol at a concentration of 3 mg / mL; the volume ratio of ethanol to propylene glycol was 3:

2. Weigh out 10 molar amounts of β-cyclodextrin relative to retinol, and add 0.7 molar amounts of stigmasterol relative to retinol. Add deionized water at 45–55°C to form a suspension and stir until homogeneous. The organic phase containing retinol was slowly added dropwise to the suspension; the reaction was continued for 4 hours under magnetic stirring, and the temperature was controlled below 50°C in the dark. Subsequently, the mixture was freeze-dried to obtain the inclusion compound powder, which was then coated with modified retinol. The resveratrol is prepared as follows: S01 Weigh 2g of lecithin, 0.8g of cholesterol, 0.1g of ceramide NP and 0.3g of phosphatidylserine, and place them in a glass beaker; add 10 mL of anhydrous ethanol until completely dissolved; S02 Add 0.1g of resveratrol to the mixture obtained in step S01, and continue stirring in a 60℃ water bath to form a uniform and transparent lipid phase; S03. Glycerin and deionized water are mixed at a mass ratio of 2:96 and the temperature is maintained at 55~60℃. Then, the transparent lipid phase is slowly added dropwise to the aqueous phase while stirring at 800 rpm to maintain uniform emulsification. After the addition is complete, continue stirring for 15-20 minutes.

2. The serum according to claim 1, characterized in that, It is used in the daily chemical industry.

Images