Nutrient for vitality of liver cell regeneration peptide

Abstract

The invention provides a nutrient for improving the activity of hepatocyte regeneration peptide, and belongs to the technical field of livestock and poultry breeding and animal health-care products, and the nutrient comprises the following components by mass: 20 g of polypeptide extracted from animal liver; 2g of vitamin B; 5 g of glycyrrhizic acid; and 6 g of silybum marianum. Through the synergistic effect of multiple components, the small molecule peptide extracted from the animal liver directly promotes liver cell regeneration in a targeted mode, the vitamin B2 strengthens the liver metabolism function, the beta-diammonium glycyrrhizinate efficiently resists inflammation and detoxifies, the silymarin extract accurately removes mycotoxin, and a complete protection chain of regeneration repair, metabolism enhancement, inflammation inhibition and toxin removal is formed; basic problems such as liver injury and metabolic disorder can be handled, complex diseases such as continuous endotoxin injury and mycotoxin accumulation which are difficult to overcome in the prior art can be solved, and the defect that the effect of a single-component product is limited is overcome.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of livestock breeding and animal health care products, in particular to a nutrient for liver cell regeneration peptide activity. BACKGROUND

[0002] In the current field of poultry and livestock breeding, the high incidence of liver diseases has become one of the key problems restricting the development of the breeding industry. Various factors such as viral and bacterial infection, endotoxin invasion, metabolic disorder, and mycotoxin influence can cause a series of liver diseases in poultry and livestock, such as liver damage, abnormal liver function, endotoxin poisoning, metabolic liver disease, mycotoxic hepatitis, liver rupture, fatty liver, and liver enlargement. These diseases not only slow down the growth rate of poultry and livestock, reduce their reproductive capacity, and lower their immunity, but also increase the mortality rate during breeding, causing huge economic losses to breeders.

[0003] In response to these liver diseases, the existing technical means have many limitations. Traditional chemical drug treatment can alleviate some symptoms in the short term, but long-term use can lead to drug resistance of pathogens, resulting in a gradual decline in treatment effectiveness. Moreover, the chemical drug residues in poultry and livestock are a major concern. These residues can enter the human body through meat, eggs, and milk products, posing a potential threat to human health, which is contrary to the current concept of green breeding and food safety.

[0004] Some single-component nutritional supplements or liver-protecting products are also difficult to meet the treatment needs of complex liver diseases due to their single efficacy. For example, some products can only have a simple detoxification effect but cannot promote the regeneration of damaged liver cells. Some products can improve liver metabolism to some extent, but they cannot control the inflammatory response caused by viral and bacterial infection. In addition, there are obvious deficiencies in the existing extraction technology when extracting polypeptides with liver cell regeneration function from animal livers. It is difficult to effectively remove impurities during the extraction process, resulting in low purity of the polypeptides and affecting their biological activity and efficacy. Moreover, improper control of extraction conditions can damage the structure of the polypeptides, rendering them unable to promote liver cell regeneration.

[0005] In addition, for more complex liver diseases such as endotoxin poisoning and mycotoxic hepatitis, the existing technology lacks comprehensive and effective intervention means. Endotoxins can continuously damage liver cells and destroy the normal physiological functions of the liver, while mycotoxins can accumulate in the liver, further exacerbating liver damage. Existing products are difficult to address these multiple damages simultaneously and cannot achieve comprehensive protection and repair of the liver. Therefore, a nutrient for liver cell regeneration peptide activity is proposed. SUMMARY

[0006] Therefore, the embodiment of the present application hopes to provide a nutrient for the activity of hepatocyte regeneration peptide to solve or alleviate the technical problems existing in the prior art, at least to provide a beneficial choice.

[0007] The object of the present application can be achieved by the following technical measures: a nutrient for the activity of hepatocyte regeneration peptide, which comprises the following components by mass:

[0008] polypeptide extracted from animal liver 20g;

[0009] vitamin B2 10g;

[0010] glycyrrhizic acid 5g;

[0011] silymarin 6g.

[0012] As further preferred of the present technical solution: the polypeptide extracted from animal liver is a small molecular peptide with hepatocyte regeneration promoting activity, the molecular weight range is 500-3000Da, the glycyrrhizic acid is β-glycyrrhizic acid diammonium salt, the purity is ≥95%, and the silymarin is silymarin extract, wherein the mass content of silymarin is ≥80%.

[0013] As further preferred of the present technical solution: the preparation method of the nutrient comprises the following steps:

[0014] S1, mixing the polypeptide extracted from animal liver, vitamin B2, glycyrrhizic acid and silymarin according to the mass ratio of claim 1, stirring at a speed of 100-150rpm under the condition of 25-30℃ and relative humidity of 40%-50% for 25-35 minutes to obtain a mixture;

[0015] S2, adding 5%-8% of anhydrous ethanol to the mixture obtained in S1, continuing to stir for 10-15 minutes to form a uniform dispersion system;

[0016] S3, placing the material obtained in S2 in a vacuum drying oven, setting the temperature to 55℃ and the vacuum degree to-0.085MPa, drying to a moisture content of ≤3%, and then cooling and crushing through an 80-100 mesh sieve, and returning the unpassed part to crushing;

[0017] S4, placing the powder obtained in S3 on a stainless steel tray and placing it in an ultraviolet sterilization cabinet, setting the wavelength to 254nm and irradiating for 35 minutes, the sterilization rate is ≥99.9%, and the tray is turned over every 10 minutes during sterilization;

[0018] S5, performing sub-packaging in a hundred-level clean area and filling with nitrogen before heat sealing.

[0019] As further preferred of the present technical solution: the anhydrous ethanol added in S2 is completely volatilized during the drying process in S3, and the residual amount is ≤0.05%.

[0020] As further preferred of the technical solution, the particle size distribution of the powder after crushing in S3 satisfies D90≤180μm.

[0021] As further preferred of the technical solution, the dose of the ultraviolet sterilization treatment in S4 is 4000-6000μW·s / cm 2 , and the polypeptide activity retention rate of the powder after sterilization is ≥95%.

[0022] As further preferred of the technical solution, the purity of the nitrogen filled in S5 is ≥99.9%, and the residual oxygen in the package is ≤0.5%.

[0023] As further preferred of the technical solution, the storage condition of the nutrient is sealed and placed in a cool and dry place, the temperature is ≤25℃, the relative humidity is ≤60%, the shelf life is 12 months, the activity retention rate of the polypeptide under this condition is ≥90%, and the content of vitamin B2 decreases ≤5%.

[0024] As further preferred of the technical solution, the specific operation mode of the nutrient when used is: added to the basic feed at a mass ratio of 1:500, the mixing time is ≥15 minutes, the coefficient of variation is ≤5%, or dissolved in drinking water at a mass concentration of 0.1%, and filtered with a 0.45μm filter before use.

[0025] As further preferred of the technical solution, for different animal species and growth stages, the recommended dosage is adjusted, for young poultry, the recommended daily dosage per kilogram of body weight is 0.05-0.1g; for adult livestock, the recommended daily dosage per kilogram of body weight is 0.1-0.2g; when the animal is in a stress state, such as transportation, group transfer, and early stage of disease infection, the dosage can be increased by 30%-50% based on the above, and the continuous use time does not exceed 21 days.

[0026] The embodiment of the present application has the following advantages due to the use of the above technical solution:

[0027] Firstly, the present application forms a complete protection chain of "regeneration repair + metabolic enhancement + inflammation inhibition + toxin removal" through the synergistic effect of multiple components, the small molecule peptides extracted from animal liver directly target to promote hepatocyte regeneration, vitamin B2 strengthens liver metabolic function, β-diammonium glycyrrhizinate efficiently anti-inflammatory and detoxification, and silymarin extract accurately removes mycotoxins, which can not only cope with basic problems such as liver damage and metabolic disorders, but also solve complex diseases such as endotoxin continuous damage and mycotoxin accumulation that are difficult to overcome in existing technologies, and make up for the short board of single component product efficacy limitation.

[0028] Secondly, the present application guarantees the reliability of the product through fine chemical process design. In the preparation process, the dose of ultraviolet sterilization is strictly controlled to ensure that the polypeptide activity retention rate is greater than or equal to 95%, the vacuum drying and nitrogen packaging technology effectively reduces the risk of component oxidation, and the polypeptide activity can still be maintained above 90% within 12 months during storage (≤25℃, humidity ≤60%), avoiding the problems of easy destruction of polypeptide structure and insufficient purity in traditional extraction technology. At the same time, the product does not contain chemical drug components, has no drug resistance hidden danger and residual risk, meets the core needs of green breeding and food safety, and provides a safe and sustainable solution for the breeding industry.

[0029] Thirdly, the present application has the convenience of flexible adaptation and the scientificity of precise regulation. In terms of use, it can be mixed into feed at a ratio of 1:500 (with high mixing uniformity), or dissolved in drinking water at a concentration of 0.1% (guaranteed clean through filter membrane), adapting to different breeding scenes. At the same time, in terms of dosage design, it provides stepwise recommended dosage for different objects such as young poultry and adult livestock and stress states, such as 0.05-0.2g / kg body weight / day, with an increase of 30%-50% under stress, which not only ensures maximum efficacy, but also avoids resource waste, greatly improving the practicality and generalizability of the product in breeding practice.

[0030] The above summary is merely intended to illustrate the present application and is not intended to limit in any way. Further aspects, embodiments and features of the present application will be readily apparent from the detailed description which follows, by reference to which, the above summary is to be interpreted. DETAILED DESCRIPTION

[0031] In the following, only certain example embodiments are simply described. As will be realized by those skilled in the art, the described embodiments can be modified in various different ways without departing from the spirit or scope of the application. Therefore, the description is considered to be essentially exemplary rather than restrictive.

[0032] It should be noted that the terms "first", "second", "symmetric", "array" and the like are only used for the purpose of distinguishing description and position description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "symmetric" and the like can explicitly or implicitly include one or more of the features; similarly, for some features that are not limited in number by the words "two", "three" and the like, it should be noted that the features also belong to explicitly or implicitly including one or more features in number;

[0033] In the present application, unless otherwise explicitly specified and limited, the terms such as "mounting", "connecting", "fixing" and the like shall be understood in a broad sense; for example, it can be fixed connection, or detachable connection, or integral molding; it can be mechanical connection, it can be direct connection, it can be welding, or it can be indirect connection through an intermediate medium, or it can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood in combination with the specific circumstances.

[0034] The embodiments of the present application are described in detail below.

[0035] A nutrient for the activity of hepatocyte regeneration peptide, the nutrient comprises the following components by mass:

[0036] Polypeptide extracted from animal liver 20g;

[0037] Vitamin B2 10g;

[0038] Glycyrrhizic acid 5g;

[0039] Silybum marianum 6g.

[0040] Specifically, the polypeptide extracted from animal liver is a small molecule peptide with hepatocyte regeneration promoting activity, the molecular weight range is 500-3000Da, the glycyrrhizic acid is β-glycyrrhizic acid diammonium salt, the purity is ≥95%, and the silybum marianum is silymarin extract, wherein the mass content of silymarin is ≥80%.

[0041] Specifically, the preparation method of the nutrient comprises the following steps:

[0042] S1, mixing the polypeptide extracted from animal liver, vitamin B2, glycyrrhizic acid and silybum marianum according to the mass ratio of claim 1, stirring at a speed of 100-150rpm under the condition of 25-30℃ and relative humidity of 40%-50% for 25-35 minutes to obtain a mixture;

[0043] S2, adding 5%-8% of anhydrous ethanol to the mixture obtained in S1, continuing to stir for 10-15 minutes to form a uniform dispersion system;

[0044] S3, placing the material obtained in S2 in a vacuum drying box, setting the temperature to 55℃ and the vacuum degree to-0.085MPa, drying until the moisture content is ≤3%, and then cooling and crushing through an 80-100 mesh sieve, and returning the unpassed part to crushing;

[0045] S4, placing the powder obtained in S3 on a stainless steel tray and placing it in an ultraviolet sterilization cabinet, setting the wavelength to 254nm and irradiating for 35 minutes, the sterilization rate is ≥99.9%, and the tray is turned over every 10 minutes during the sterilization process;

[0046] S5, sub-packaging in a hundred-level clean area and heat-sealing after filling with nitrogen.

[0047] Specifically, the anhydrous ethanol added in S2 is completely volatilized in the drying process in S3, and the residual amount is ≤0.05%.

[0048] Specifically, the particle size distribution of the powder after crushing in S3 meets: D90≤180μm.

[0049] Specifically, the dose of the ultraviolet sterilization treatment in S4 is 4000-6000μW·s / cm 2 , and the polypeptide activity retention rate of the powder after sterilization is ≥95%.

[0050] Specifically, the purity of the nitrogen filled in S5 is ≥99.9%, and the residual oxygen in the package is ≤0.5%.

[0051] Specifically, the storage condition of the nutrient is sealed and placed in a cool and dry place, the temperature is ≤25℃, and the relative humidity is ≤60%, the shelf life is 12 months, the activity retention rate of the polypeptide under this condition is ≥90%, and the content of vitamin B2 decreases ≤5%.

[0052] Specifically, the specific operation mode of the nutrient when used is: added to the basic feed at a mass ratio of 1:500, the mixing time is ≥15 minutes, the coefficient of variation is ≤5%, or dissolved in drinking water at a mass concentration of 0.1%, and filtered with a 0.45μm filter before use.

[0053] Specifically, for different animal species and growth stages, the recommended dosage is adjusted, for young poultry, the recommended dosage per kilogram of body weight per day is 0.05-0.1 grams, for adult livestock, the recommended dosage per kilogram of body weight per day is 0.1-0.2 grams, and in the case of stress, such as transportation, group transfer, and early stage of disease infection, the dosage can be increased by 30%-50% based on the above, and the continuous use time does not exceed 21 days.

[0054] The following are several specific embodiments of the application

[0055] Example 1: routine breeding application of young poultry

[0056] Test object: 200 healthy broilers of 30 days old were selected and randomly divided into a control group and a test group, 100 in each group, and the test period was 45 days, wherein the control group was basic feed, and the test group was basic feed and the nutrient.

[0057] Usage: The nutrient was mixed into the basic feed at a mass ratio of 1:500, and the mixing time was 20 minutes.

[0058] Dose standard: the daily dose of young poultry per kilogram of body weight was 0.08 grams.

[0059] Test results: The liver health indicators of the test group were better than those of the control group, the incidence of liver damage was reduced by 62%; the average daily gain was increased by 12% compared with the control group, and the feed conversion ratio was decreased by 8%; at the end of the test, the polypeptide activity retention rate in the liver tissue was 96%, and no drug residues were found.

[0060] Example 2: Application during the stress period of adult livestock

[0061] Test subjects: 10 adult cows in a state of transportation stress were selected and randomly divided into a control group and a test group, 5 in each group, with a test period of 14 days. The control group was fed with basic feed and regular drinking water, and the test group was fed with basic feed and drinking water containing the nutrient.

[0062] Usage: Dissolve the nutrient in drinking water at a mass concentration of 0.1%, and filter it through a 0.45 μm filter membrane before use.

[0063] Dose standard: The basic dose for adult livestock is 0.15 g / kg body weight / day, which is increased by 40% under stress, i.e. adjusted to 0.21 g / kg body weight / day, and used continuously for 14 days.

[0064] Test results: The alanine transaminase level of the test group during the stress period was reduced by 45% compared with the control group, and the mycotoxin content in feces was reduced by 58%; the milk yield was reduced by less than 5% compared with the control group, of which the control group was reduced by 18%; after the stress ended, the expression amount of liver regeneration related genes was increased by 30% compared with the control group, and the recovery speed was accelerated.

[0065] Example 3: Application during the early stage of disease infection of young livestock

[0066] Test subjects: 15 45-day-old piglets diagnosed with early stage of mycotic hepatitis were selected and randomly divided into a control group and a test group, 7-8 in each group, with a test period of 21 days. The control group was treated with basic treatment, and the test group was treated with basic treatment and the nutrient.

[0067] Usage: Mix into feed at a ratio of 1:500, and dissolve in drinking water at a concentration of 0.1%.

[0068] Dose standard: Refer to the standard for young poultry (0.05-0.1 g / kg body weight / day), and increase by 50% during the early stage of disease, i.e. use 0.075-0.15 g / kg body weight / day.

[0069] Test results: The appetite recovery rate of the test group of piglets within 7 days was 85%, of which the control group was 50%, and the cure rate within 21 days was 92%, while the control group was 60%; liver biopsy showed that the liver cell regeneration area of the test group was increased by 60% compared with the control group, and the inflammatory cell infiltration was reduced by 40%; throughout the process, the polypeptide activity did not decrease, the retention rate was more than 95%, and there was no adverse reaction.

[0070] The above-mentioned embodiments verify that the present application has the following advantages:

[0071] I. Efficacy level: Through the synergistic effect of multiple components, liver health is significantly improved, liver cell regeneration is promoted, disease incidence is reduced, and production performance is improved;

[0072] II. Applicability level: Two usage methods (feed addition / dissolution in drinking water) combined with dosage adjustment rules (age / stress state) adapt to different animal types and physiological states;

[0073] III. Safety level: No residue, no drug resistance, and process control ensures the stability of polypeptide activity and ingredients, meeting the needs of green breeding;

[0074] In summary, the nutrient of the present application can effectively solve the limitations of the prior art in practical application, and provides a reliable solution for animal liver health management.

[0075] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can easily think of various changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A nutrient for liver cell regeneration peptide vitality, characterized in that, This nutrient contains the following ingredients by mass: 20 grams of peptides extracted from animal liver; Vitamin B2 10g; Glycyrrhizic acid 5g; 6 grams of milk thistle.

2. A nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 1, characterized in that: The polypeptide extracted from the animal liver is a small molecule peptide with liver cell regeneration promoting activity, with a molecular weight range of 500-3000Da; the glycyrrhizic acid is β-glycyrrhizic acid diammonium salt, with a purity of ≥95%; and the milk thistle is a silymarin extract, wherein the mass content of silymarin is ≥80%.

3. A nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 1, characterized in that: The preparation method of the nutrient comprises the following steps: S1. Mixing the polypeptide extracted from animal liver, vitamin B2, glycyrrhizic acid, and milk thistle according to the mass ratio of claim 1, stirring at a speed of 100-150 rpm for 25-35 minutes at 25-30° C. and a relative humidity of 40%-50% to obtain a mixed material; S2. Add 5%-8% of anhydrous ethanol to the mixture obtained in S1, and continue stirring for 10-15 minutes to form a uniform dispersion system; S3. Place the material obtained in S2 in a vacuum drying oven, set the temperature to 55°C and the vacuum degree to -0.085MPa, dry until the moisture content is ≤3%, and then grind it through an 80-100 mesh sieve after cooling. The part that does not pass through the sieve is returned for grinding; S4. Spread the powder obtained in S3 on a stainless steel tray and place it in an ultraviolet sterilizer. Set the wavelength to 254nm and irradiate for 35 minutes. The sterilization rate should be ≥99.9%. Turn the tray every 10 minutes during the sterilization process. S5. Pack in a Class 100 clean area, fill with nitrogen and then heat seal.

4. A nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 3, characterized in that: The anhydrous ethanol added in S2 is completely volatilized during the drying process in S3, and the residual amount is ≤0.05%.

5. The nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 3, characterized in that: The particle size distribution of the powder after pulverization in S3 satisfies: D90≤180 μm.

6. A nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 3, characterized in that: The dosage of ultraviolet sterilization in S4 is 4000-6000 μW·s / cm 2 , and the polypeptide activity retention rate of the powder after sterilization is ≥95%.

7. The nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 1, characterized in that: The purity of the nitrogen filled in S5 is ≥99.9%, and the residual oxygen content in the package is ≤0.5%.

8. The nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 1, characterized in that: The storage conditions of the nutrient are to be sealed and placed in a cool and dry place, with a temperature of ≤25°C and a relative humidity of ≤60%. The shelf life is 12 months. Under these conditions, the activity retention rate of the polypeptide is ≥90% and the vitamin B2 content decreases by ≤5%.

9. The nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 1, characterized in that: The specific operation method of the nutrient when used is: add it to the basic feed at a mass ratio of 1:500, mix it for ≥15 minutes, and the coefficient of variation is ≤5%, or dissolve it in drinking water at a mass concentration of 0.1% and filter it with a 0.45μm filter membrane before use.

10. The nutrient for enhancing the vitality of hepatocyte regeneration peptide according to claim 1, characterized in that: The recommended dosage is adjusted for different animal species and growth stages. For young poultry, the recommended daily dosage is 0.05-0.1 grams per kilogram of body weight; for adult livestock, the recommended daily dosage is 0.1-0.2 grams per kilogram of body weight; when the animal is in a stressful state, such as during transportation, group transfer, and the early stages of disease infection, the dosage can be increased by 30%-50% on the above basis, and the continuous use time shall not exceed 21 days.