Mesona chinensis juice extraction method, Mesona chinensis beverage and preparation method of Mesona chinensis beverage
Through water extraction with specific alkali concentration, enzymatic treatment and cascade filtration combined with activated carbon-resin decolorization, the problem of dark brown color and bitterness of grass jelly extract was solved, and efficient decolorization and polysaccharide retention were achieved, making it suitable for high-end beverages and dairy products.
Patent Information
- Application Number
- CN202510989480.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-17
- Publication Date
- 2025-10-10
AI Technical Summary
The existing herbal jelly extraction process results in a dark brown and bitter extract, making it difficult to use in high-quality beverages and dairy products. In addition, the decolorization process is costly and has a high polysaccharide loss rate, which cannot meet the needs of clean-label foods.
After water extraction with a specific alkaline concentration, it is subjected to enzymatic treatment, nanofiltration membrane and reverse osmosis membrane cascade filtration, combined with activated carbon-resin decolorization, and the pigment is encapsulated using a combination of cellulase and pectinase. Nanofiltration + reverse osmosis concentration filtration retains the polysaccharide, and finally decolorization and adsorption are performed by activated carbon-resin.
It achieves efficient decolorization and odor removal, with a decolorization rate of ≥92% and a polysaccharide loss rate of ≤8%. The resulting grass jelly juice has extremely light color and native flavor, and can be used in high-end beverages and dairy products.
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of food preparation, and in particular to a grass jelly juice extraction method, a grass jelly beverage and a preparation method thereof. Background Art
[0002] Mesona chinensis Benth., a traditional medicinal and edible plant, is rich in functional ingredients such as herbal polysaccharides and flavonoids. It exhibits antioxidant and hypoglycemic properties and is widely used in the food industry. Traditional herbal jelly processing often utilizes an alkaline water extraction process, where the colloidal components are extracted by adding sodium hydroxide and boiling. However, this process often results in a dark brown extract with a distinct bitter taste, primarily due to the release of pigments (such as chlorophyll degradation products) and alkaloids from the collapse of the plant cell walls. These sensory deficiencies severely limit the application of herbal jelly extracts in light-colored food systems, such as high-quality beverages and dairy products.
[0003] To improve the sensory quality of herbal jelly extract, the industry generally employs physical or chemical decolorization techniques. Activated carbon adsorption is widely used due to its ease of operation, but its non-selective adsorption of high-molecular-weight polysaccharides often results in functional component losses exceeding 20%. While ion exchange resins can improve decolorization efficiency (approximately 60% decolorization), they still cannot prevent polysaccharides from being trapped due to charge, resulting in losses exceeding 25%. More critically, existing decolorization processes rely on multiple treatments or high adsorbent dosages, which not only increases production costs but also introduces the risk of secondary residues, making it difficult to meet the demands of clean-label foods.
[0004] Therefore, developing a herbal jelly deep processing technology that can simultaneously achieve efficient decolorization and odor removal and high polysaccharide retention rate has become the core demand to break through the barriers to industrial upgrading. Summary of the Invention
[0005] The technical problem to be solved by the present invention is to develop a grass jelly deep processing technology that can simultaneously achieve efficient decolorization and deodorization and high polysaccharide retention rate, and specifically provide a grass jelly juice extraction method and a grass jelly beverage preparation method.
[0006] In order to solve the above problems, the present invention proposes the following technical solutions:
[0007] A method for extracting grass jelly juice comprises the following steps:
[0008] S1. Add deionized water to the herbal jelly raw materials at a material-water ratio of 1:15-20, add 0.1-0.8% by mass of sodium hydroxide, and boil;
[0009] S2, keep boiling for 2-5 hours, then cool to below 60°C, pass through a 100-300 mesh sieve to obtain a coarse filtrate;
[0010] S3, after enzymatic hydrolysis, the crude filtrate is filtered through a nanofiltration membrane and then through a reverse osmosis membrane to obtain a concentrated solution;
[0011] S4. Passing the concentrated liquid through an activated carbon adsorption column and a resin exchange column in sequence for decolorization and deodorization to obtain grass jelly juice.
[0012] Furthermore, nanofiltration membrane filtration uses a ceramic membrane with a pore size of 180-200 nm and an operating pressure of 0.3-0.6 MPa.
[0013] Furthermore, the enzyme used for the enzymatic hydrolysis in step S3 is a mixture of cellulase and pectinase; wherein the added amount of cellulase is 3%-3.5% by mass, and the added amount of pectinase is 0.5%-1.5% by mass.
[0014] Furthermore, in step S3, the enzymatic hydrolysis treatment is performed by adding the enzyme, stirring at 45-50° C. for 100-150 minutes, and then filtering through a 50-100 mesh sieve.
[0015] Furthermore, the solid content by mass in the concentrated solution obtained in step S3 is ≥30%.
[0016] Furthermore, step S4 also includes maintaining the temperature of the concentrated liquid at 40±2° C. and adjusting the pH to match the conditions of the resin exchange column.
[0017] Furthermore, the chromaticity OD of the finally obtained grass jelly juice is less than 0.05.
[0018] Furthermore, the step S1 further includes pre-processing the raw materials of the grass jelly:
[0019] Cut the grass jelly raw materials into 3-5cm lengths, remove impurities, and control the moisture content below 12%.
[0020] The present invention also provides a grass jelly beverage, which comprises 18-25% grass jelly juice, 5-10% fruit juice, 6-8% white sugar and 55-70% RO water in percentage by mass. The grass jelly juice is prepared by the grass jelly juice extraction method described above.
[0021] The present invention also provides a method for preparing a grass jelly beverage, comprising: uniformly mixing, by mass percentage, 18-25% of grass jelly juice, 5-10% of fruit juice, 6-8% of white sugar, and 55-70% of RO water to obtain a mixed liquid; canning and sterilizing the mixed liquid; and the grass jelly juice is prepared by the grass jelly juice extraction method described above.
[0022] Compared with the prior art, the present invention can achieve the following technical effects:
[0023] The present invention provides a method for extracting grass jelly juice, comprising the steps of extracting the grass jelly raw material with water at a specific alkali concentration, followed by coarse filtration, enzymatic hydrolysis, and cascade concentration and filtration using nanofiltration and reverse osmosis membranes, followed by decolorization and deodorization with activated carbon and resin to obtain the grass jelly juice. The method utilizes a specific concentration of alkaline water for extraction to efficiently dissolve the colloid in the grass jelly raw material, inhibiting excessive pigment release and paving the way for subsequent purification. The enzymatic hydrolysis utilizes a combination of cellulase and pectinase to specifically cleave the encapsulated pigment, fully exposing the pigment molecules. The method utilizes a nanofiltration combined with reverse osmosis concentration and filtration method to preferentially retain polysaccharide colloids with a molecular weight greater than 180 nm, achieving a solids concentration of ≥30% in the concentrate and significantly reducing subsequent processing volume. These steps synergistically significantly reduce the difficulty of decolorization, ultimately achieving a decolorization rate of ≥92% through activated carbon and resin decolorization and adsorption, while controlling the polysaccharide loss rate to ≤8%, representing a qualitative improvement over conventional processes (decolorization rate ≤60% and polysaccharide loss ≥25%).
[0024] The proposed method for extracting grass jelly juice offers a simple and low-cost process. It utilizes a combination of enzymatic hydrolysis and nanofiltration plus reverse osmosis concentration and filtration, which helps reduce the amount of adsorbent required. Furthermore, due to the improved polysaccharide retention, the yield of active ingredients increases with the same raw materials. The resulting grass jelly juice exhibits a very light color (OD < 0.05) and a natural flavor, completely eliminating bitterness and astringency. It can be directly used in high-end products such as light-colored beverages and dairy products. DETAILED DESCRIPTION
[0025] The technical solutions in the embodiments are described clearly and completely below. Obviously, the embodiments described below are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are also within the scope of protection of the present invention.
[0026] It will be understood that when used in this specification and the appended claims, the terms “comprises” and “comprising” indicate the presence of described features, integers, steps, operations, elements and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or groups thereof.
[0027] It should also be understood that the terms used in this description of the embodiments of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the embodiments of the present invention. As used in the description of the embodiments of the present invention and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms unless the context clearly indicates otherwise.
[0028] Example 1
[0029] The embodiment of the present invention provides a method for extracting grass jelly juice, comprising the following steps:
[0030] S1. Cut the grass jelly raw material into 3-5 cm lengths, remove impurities, and make the moisture content below 12%; then add deionized water at a material-water ratio of 1:18 to the grass jelly raw material, add 0.5% by mass fraction of sodium hydroxide, and boil.
[0031] S2. Keep boiling for 4 hours, then cool to below 60°C, pass through a 200-mesh sieve, and centrifuge at 3500 r / min for 20 minutes to obtain a coarse filtrate.
[0032] S3. After centrifuging the crude filtrate, an enzyme is added and stirred at 50° C. for 120 minutes for enzymatic hydrolysis. The filtrate is then filtered through an 80-mesh sieve, and subsequently filtered through a nanofiltration membrane and a reverse osmosis membrane to obtain a concentrate having a solids content of ≥30% by weight. The nanofiltration membrane is filtered using a ceramic membrane with a pore size of 200 nm and an operating pressure of 0.5 MPa.
[0033] S4. Maintain the temperature of the concentrated liquid at 40±2° C., adjust the pH to match the conditions of the resin exchange column, and pass the filtrate through an activated carbon adsorption column and a resin exchange column in sequence at a flow rate of 4 BV / h (column volume / hour) for decolorization and deodorization to obtain grass jelly juice.
[0034] In this embodiment, the enzyme used for the enzymatic hydrolysis in step S3 is a mixture of cellulase and pectinase; wherein, the added amount of cellulase is 3% by mass, and the added amount of pectinase is 1.0% by mass.
[0035] In this embodiment, the resin used in the resin exchange column in step S4 is a sulfonic acid-amino bifunctional resin (LX-68), and the pH of the filtrate is adjusted to 4.0.
[0036] It is understood that the resin exchange column needs to be pretreated and loaded, wherein the pretreatment operation includes:
[0037] Sieve the resin (particle size 0.3-1.2mm), remove broken particles, and rinse with deionized water until there are no impurities; then activate the resin by alternating acid / base:
[0038] For cationic resin: soak in 4% HCl for 4 hours for activation, then wash with deionized water until the pH is 6.0.
[0039] For anion resin: activate it by soaking in 4% NaOH for 4 hours, and then wash it with deionized water until the pH is 8.0.
[0040] The loading operation is as follows:
[0041] Pre-fill the bottom of the exchange column with deionized water to 1 / 3 of its height, wet-load the resin (avoid bubbles), and fill the resin so that the ratio of resin bed height to diameter is ≥3:1, leaving 20% expansion space at the top.
[0042] Example 2
[0043] The embodiment of the present invention provides a method for extracting grass jelly juice, comprising the following steps:
[0044] S1. Cut the grass jelly raw material into 3-5 cm lengths, remove impurities, and make the moisture content below 12%; then add deionized water at a material-water ratio of 1:18 to the grass jelly raw material, add 0.5% by mass fraction of sodium hydroxide, and boil.
[0045] S2. Keep boiling for 4 hours, then cool to below 60°C, pass through a 200-mesh sieve, and centrifuge at 3500 r / min for 20 minutes to obtain a coarse filtrate.
[0046] S3. After centrifuging the crude filtrate, an enzyme was added and stirred at 50° C. for 120 minutes for enzymatic hydrolysis. The filtrate was then filtered through an 80-mesh sieve, and then filtered through a nanofiltration membrane and a reverse osmosis membrane to obtain a concentrate having a solids content of 32% by weight. The nanofiltration membrane was filtered using a ceramic membrane with a pore size of 180 nm and an operating pressure of 0.5 MPa.
[0047] S4. Maintain the temperature of the concentrated liquid at 40±2° C., adjust the pH to match the conditions of the resin exchange column, and pass the filtrate through an activated carbon adsorption column and a resin exchange column in sequence at a flow rate of 4 BV / h (column volume / hour) to decolorize and remove odor to obtain grass jelly juice.
[0048] In this embodiment, the enzyme used for the enzymatic hydrolysis in step S3 is a mixture of cellulase and pectinase; wherein, the added amount of cellulase is 3.5% by mass, and the added amount of pectinase is 0.8% by mass.
[0049] In this embodiment, the resin used in the resin exchange column of step S4 is a mixture of a strongly acidic cationic resin (model 001×7 (styrene-divinylbenzene copolymer, sulfonic acid group)) and an anionic resin (model 201×7 (quaternary ammonium group)), and the pH of the filtrate is adjusted to 4.0.
[0050] Example 3
[0051] The difference between this embodiment and embodiment 1 is that in step S1, the mass fraction of the sodium hydroxide used is 0.2%, and the remaining operations are the same as those in embodiment 1.
[0052] Example 4
[0053] The difference between this embodiment and embodiment 1 is that in step S1, the mass fraction of the sodium hydroxide used is 0.8%, and the remaining operations are the same as those in embodiment 1.
[0054] Comparative Example 1
[0055] The comparative example of the present invention provides a method for extracting grass jelly juice, comprising the following steps:
[0056] S1. Cut the grass jelly raw material into 3-5 cm lengths, remove impurities, and make the moisture content below 12%; then add deionized water at a material-water ratio of 1:18 to the grass jelly raw material, add 0.5% by mass fraction of sodium hydroxide, and boil.
[0057] S2. Keep boiling for 4 hours, then cool to below 60°C, pass through a 200-mesh sieve, and centrifuge at 3500 r / min for 20 minutes to obtain a coarse filtrate.
[0058] S3. Centrifuge the crude filtrate and then perform nanofiltration and reverse osmosis filtration to obtain a concentrate, wherein the solid content of the concentrate is ≥30%. The nanofiltration membrane uses a ceramic membrane with a pore size of 180 nm and an operating pressure of 0.5 MPa.
[0059] S4. Maintaining the temperature of the concentrated liquid at 40±2° C., passing the concentrated liquid through an activated carbon adsorption column at a flow rate of 4 BV / h (column volume / hour) for decolorization and deodorization to obtain grass jelly juice.
[0060] The remaining operations of this comparative example are the same as those of Example 1.
[0061] Comparative Example 2
[0062] The comparative example of the present invention provides a method for extracting grass jelly juice, comprising the following steps:
[0063] S1. Cut the grass jelly raw material into 3-5 cm lengths, remove impurities, and make the moisture content below 12%; then add deionized water at a material-water ratio of 1:18 to the grass jelly raw material, add 0.5% by mass fraction of sodium hydroxide, and boil.
[0064] S2. Keep boiling for 4 hours, then cool to below 60°C, pass through a 200-mesh sieve, and centrifuge at 3500 r / min for 20 minutes to obtain a coarse filtrate.
[0065] S3. Centrifuge the crude filtrate and then perform nanofiltration and reverse osmosis filtration to obtain a concentrate, wherein the solid content of the concentrate is ≥30%. The nanofiltration membrane uses a ceramic membrane with a pore size of 180 nm and an operating pressure of 0.5 MPa.
[0066] S4. Maintaining the temperature of the concentrated liquid at 40±2° C., adjusting the pH to match the conditions of the resin exchange column, and passing the filtrate through the resin exchange column at a flow rate of 4 BV / h (column volume / hour) for decolorization and deodorization to obtain grass jelly juice.
[0067] The resin type of this comparative example is the same as that of Example 1, and the rest of the operations are the same as those of Example 1.
[0068] Comparative Example 3
[0069] The comparative example of the present invention provides a method for extracting grass jelly juice, comprising the following steps:
[0070] S1. Cut the grass jelly raw material into 3-5 cm lengths, remove impurities, and make the moisture content below 12%; then add deionized water at a material-water ratio of 1:18 to the grass jelly raw material, add 0.5% by mass fraction of sodium hydroxide, and boil.
[0071] S2. Keep boiling for 4 hours, then cool to below 60°C, pass through a 200-mesh sieve, and centrifuge at 3500 r / min for 20 minutes to obtain a coarse filtrate.
[0072] S3. Centrifuge the crude filtrate and then perform nanofiltration and reverse osmosis filtration to obtain a concentrate, wherein the solid content of the concentrate is ≥30%. The nanofiltration membrane uses a ceramic membrane with a pore size of 180 nm and an operating pressure of 1.0 MPa.
[0073] S4. Maintain the temperature of the concentrated liquid at 40±2° C., adjust the pH to match the conditions of the resin exchange column, and pass the filtrate through an activated carbon adsorption column and a resin exchange column in sequence at a flow rate of 4 BV / h (column volume / hour) to decolorize and remove odor to obtain grass jelly juice.
[0074] The enzyme and resin types in this comparative example are the same as those in Example 1, and the remaining operations are the same as those in Example 1.
[0075] Comparative Example 4
[0076] The comparative example of the present invention provides a method for extracting grass jelly juice, comprising the following steps:
[0077] S1. Cut the grass jelly raw material into 3-5 cm lengths, remove impurities, and make the moisture content below 12%; then add deionized water at a material-water ratio of 1:18 to the grass jelly raw material, add 0.5% by mass fraction of sodium hydroxide, and boil.
[0078] S2. Keep boiling for 4 hours, then cool to below 60°C, pass through a 200-mesh sieve, and centrifuge at 3500 r / min for 20 minutes to obtain a coarse filtrate.
[0079] S3. After centrifuging the crude filtrate, an enzyme is added and stirred at 50° C. for 120 minutes for enzymatic hydrolysis. The filtrate is then filtered through an 80-mesh sieve, and subsequently filtered through a nanofiltration membrane and a reverse osmosis membrane to obtain a concentrate having a solids content of ≥30% by weight. The nanofiltration membrane is filtered using a ceramic membrane with a pore size of 180 nm and an operating pressure of 0.5 MPa.
[0080] S4. Maintaining the temperature of the concentrated liquid at 40±2° C., adjusting the pH to match the conditions of the resin exchange column, and passing the filtrate through the resin exchange column at a flow rate of 4 BV / h (column volume / hour) for decolorization and deodorization to obtain grass jelly juice.
[0081] In this comparative example, the enzyme used in the enzymatic hydrolysis of step S3 is cellulase, and the other operations are the same as those in Example 1. The amount of cellulase added is 4.0% by mass.
[0082] Comparative Example 5
[0083] The difference between this comparative example and comparative example 4 is that the enzyme used for the enzymatic hydrolysis in step S4 is pectinase, and the added amount is 4.0% by mass.
[0084] The decolorization rate, polysaccharide loss rate and chromaticity of the grass jelly juice obtained in Examples 1-2 and Comparative Examples 1-5 were tested, and the results are shown in Table 1.
[0085] Testing standards:
[0086] Chroma: Refer to GB / T 30648.2-2015 Food Colorimetry, measured at 420nm wavelength;
[0087] Decolorization rate = (1-OD value after decolorization / initial OD value of concentrate) × 100% (1-initial OD value of concentrate / OD value after decolorization) × 100% (initial OD = 0.80);
[0088] Polysaccharide loss rate: measured and calculated according to the aforementioned national standard phenol-sulfuric acid method.
[0089] Table 1 Test results of the grass jelly juice obtained in Examples 1-2 and Comparative Examples 1-2
[0090] Test items Chroma Decolorization rate Polysaccharide loss rate color Example 1 0.02±0.003 95.4±0.5 6.3±0.3 Colorless and transparent Example 2 0.04±0.005 93.1±0.8 7.9±0.4 Nearly colorless Example 3 0.02±0.003 95.0±0.5 15±0.5 Colorless and transparent Example 4 0.06±0.003 85.0±0.5 10±0.5 light yellow Comparative Example 1 0.38±0.02 52.6±1.8 21.3±0.9 light yellow Comparative Example 2 0.29±0.03 63.8±2.1 26.1±1.1 light yellow Comparative Example 3 0.17±0.01 82.5±1.5 18.7±0.7 light yellow Comparative Example 4 0.43±0.02 70.3±0.9 12.3±1.2 light yellow Comparative Example 5 0.53±0.02 75.3±0.1 16.9±1.5 light yellow
[0091] As can be seen from the results in Table 1, the decolorization effects of comparative examples 1-3 without enzymatic hydrolysis were not good. This is because the combination of cellulase + pectinase can completely decompose the cell wall residues of the grass jelly, release the encapsulated pigment molecules (such as chlorophyll derivatives), and make the adsorbent more efficient.
[0092] Comparative Example 1 uses traditional activated carbon adsorption method, the specific surface area is limited, and macromolecular polysaccharides are preferentially adsorbed, resulting in a high loss rate; Comparative Example 2 is a traditional single resin adsorption method, and the pigment adsorption capacity is limited.
[0093] The difference between Example 1 and Example 2 is that different resin materials are used, and the effect of Example 1 is the best, which is due to the fact that the sulfonic acid group adsorbs alkaline bitter substances (such as alkaloids); the amino group targets and binds anionic pigments (such as demethylated chlorophyllin); and the dual function avoids non-specific adsorption of neutral polysaccharides.
[0094] As can be seen from Comparative Examples 4-5, more than 80% of the pigments (such as chlorophyll derivatives and melanoidins) and bitter substances (alkaloids) in the genus are tightly wrapped in the cellulose-pectin network; the use of cellulase can open the cell wall'skeleton', and pectinase can assist in dissolving the intercellular 'adhesive' to fully expose the wrapped pigment molecules; using a single enzyme has the problem of poor effect, and only when the two are compounded, the pigment particle size is reduced from >500 nm to 50-100 nm after enzymatic hydrolysis, which is more easily captured by the subsequent adsorbent, and the resin treatment time can also be shortened to prevent polysaccharides from being adsorbed due to long residence time. At the same time, the enzymatic hydrolysis process of the embodiment of the present application at 50℃ can effectively avoid the degradation of the polysaccharides (molecular weight 200-500 kDa) in the genus.
[0095] As can be seen from Examples 3-4, when the concentration of sodium hydroxide is less than 0.5%, the extraction rate of the polysaccharides in the genus is reduced by about 10%. When the concentration is about 0.8%, the OD value increases significantly, increasing the difficulty of decolorization, and the extraction rate of the polysaccharides in the genus does not increase significantly.
[0096] Example 5
[0097] The embodiment of the present application provides a genus beverage, which comprises 20% of genus juice, 8% of fruit juice, 8% of white sugar and 64% of RO water by mass percentage; the genus juice is obtained by the genus juice extraction method of Example 1.
[0098] The embodiment of the present application also provides a preparation method of the genus beverage, which comprises: uniformly mixing 20% of genus juice, 8% of fruit juice, 8% of white sugar and 64% of RO water by mass percentage to obtain a blending liquid; and performing canning and sterilization on the blending liquid; the genus juice is obtained by the genus juice extraction method.
[0099] Example 6
[0100] The embodiment of the present invention provides a grass jelly beverage, which comprises, by mass percentage, 20% grass jelly juice, 8% fruit juice, 8% white sugar, and 64% RO water; the grass jelly juice is the grass jelly juice prepared in Example 1.
[0101] The embodiment of the present invention also provides a method for preparing a grass jelly beverage, comprising: uniformly mixing 20% grass jelly juice, 8% fruit juice, 8% sugar, and 64% RO water by mass to obtain a mixed liquid; and canning and sterilizing the mixed liquid.
[0102] The sterilization method is sterilization at 90°C for 10 minutes.
[0103] Example 7
[0104] The embodiment of the present invention provides a grass jelly beverage, which comprises, by mass percentage, 20% grass jelly juice, 8% fruit juice, 6% white sugar, and 66% RO water; the grass jelly juice is the grass jelly juice prepared in Example 1.
[0105] The embodiment of the present invention also provides a method for preparing a grass jelly beverage, comprising: uniformly mixing 20% grass jelly juice, 8% fruit juice, 6% sugar, and 66% RO water by mass to obtain a mixed liquid; and canning and sterilizing the mixed liquid.
[0106] The sterilization method is sterilization at 90°C for 10 minutes.
[0107] The present invention provides a method for extracting grass jelly juice, comprising the steps of extracting the grass jelly raw material with water at a specific alkali concentration, followed by enzymatic hydrolysis, cascade filtration using nanofiltration and reverse osmosis membranes, and finally decolorization and deodorization with activated carbon and resin to obtain the grass jelly juice. The alkaline water extraction with the specific concentration efficiently dissolves the colloids in the grass jelly raw material, inhibiting excessive pigment release and paving the way for subsequent purification. The enzymatic hydrolysis uses a combination of cellulase and pectinase to specifically cleave the encapsulated pigments, fully exposing the pigment molecules. The method utilizes nanofiltration combined with reverse osmosis concentration and filtration to preferentially retain polysaccharide colloids with a molecular weight greater than 180 nm, achieving a solids concentration of ≥30% in the concentrate and significantly reducing the subsequent processing volume. These steps synergistically significantly reduce the difficulty of decolorization, ultimately achieving a decolorization rate of ≥92% through activated carbon and resin decolorization and adsorption, with a polysaccharide loss rate of ≤8%, representing a qualitative improvement over conventional processes (decolorization rate ≤60% and polysaccharide loss ≥25%).
[0108] The proposed method for extracting grass jelly juice offers a simple and low-cost process. It utilizes a combination of enzymatic hydrolysis and nanofiltration plus reverse osmosis concentration and filtration, which helps reduce the amount of adsorbent required. Furthermore, due to the improved polysaccharide retention, the yield of active ingredients increases with the same raw materials. The resulting grass jelly juice exhibits a very light color (OD < 0.05) and a natural flavor, completely eliminating bitterness and astringency. It can be directly used in high-end products such as light-colored beverages and dairy products.
[0109] In the above embodiments, the description of each embodiment has its own focus. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.
[0110] The above description is a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present invention, and such modifications or substitutions are intended to be within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.
Claims
1. A method for extracting grass jelly juice, characterized in that: The following steps are involved: S1. Add deionized water to the herbal jelly raw materials at a material-water ratio of 1:15-20, add 0.1-0.8% by mass of sodium hydroxide, and boil; S2, keep boiling for 2-5 hours, then cool to below 60°C, pass through a 100-300 mesh sieve to obtain a coarse filtrate; S3, after enzymatic hydrolysis, the crude filtrate is filtered through a nanofiltration membrane and then through a reverse osmosis membrane to obtain a concentrated solution; S4. Passing the concentrated liquid through an activated carbon adsorption column and a resin exchange column in sequence for decolorization and deodorization to obtain grass jelly juice.
2. The method for extracting grass jelly juice according to claim 1, wherein: Nanofiltration membrane filtration uses a ceramic membrane with a pore size of 180-200nm and an operating pressure of 0.3-0.6MPa.
3. The method for extracting grass jelly juice according to claim 1, wherein: The enzyme used for the enzymatic hydrolysis in step S3 is a mixture of cellulase and pectinase; wherein the added amount of cellulase is 3%-3.5% by mass, and the added amount of pectinase is 0.5%-1.5% by mass.
4. The method for extracting grass jelly juice according to claim 1, wherein In step S3, the enzymatic hydrolysis treatment is performed by adding the enzyme, stirring at 45-50° C. for 100-150 minutes, and then filtering through a 50-100 mesh sieve.
5. The method for extracting grass jelly juice according to claim 1, wherein: The concentrated solution obtained in step S3 has a solid content of ≥30% by mass.
6. The method for extracting grass jelly juice according to claim 1, wherein: Step S4 also includes maintaining the temperature of the concentrated solution at 40±2° C. and adjusting the pH to match the conditions of the resin exchange column.
7. The method for extracting grass jelly juice according to claim 1, wherein: The chromaticity OD of the finally obtained grass jelly juice is less than 0.
05.
8. The method for extracting grass jelly juice according to claim 1, wherein: The step S1 also includes pre-processing the raw materials of the grass jelly: Cut the grass jelly raw materials into 3-5cm lengths, remove impurities, and control the moisture content below 12%.
9. A grass jelly beverage, characterized in that: Calculated by mass percentage, it comprises 18-25% of grass jelly juice, 5-10% of fruit juice, 6-8% of white sugar, and 55-70% of RO water; the grass jelly juice is prepared by the grass jelly juice extraction method according to any one of claims 1-8.
10. A method for preparing a grass jelly beverage, characterized in that: include: 18-25% of grass jelly juice, 5-10% of fruit juice, 6-8% of white sugar and 55-70% of RO water are mixed uniformly by mass to obtain a prepared liquid; the prepared liquid is canned and sterilized; the grass jelly juice is prepared by the grass jelly juice extraction method according to any one of claims 1-8.