A highly effective anti-allergic, soothing, and repairing composition, its preparation method, and its application.

Abstract

This application belongs to the field of cosmetic manufacturing technology and discloses a highly effective anti-allergic, soothing, and repairing composition, its preparation method, and its application. The composition contains peony extract, peach resin extract, and purslane extract, with a mass ratio of 0.008–2.4:0.02–0.4:0.000008–0.48. This application enhances the composition's ability to inhibit inflammatory factors and provides a certain degree of improvement in the skin's barrier function through the combination of these plant extracts.

Description

Technical Field

[0001] This application relates to the field of cosmetic manufacturing technology, and in particular to a highly effective anti-allergic, soothing, and repairing composition, its preparation method, and its application. Background Technology

[0002] The skin is the largest organ in the human body, playing a vital protective role. When skin cells are damaged and their resistance is weakened, various allergic reactions can occur when exposed to external stimuli, such as during seasonal changes. Over time, the skin becomes abnormally fragile, developing into sensitive skin. Sensitive skin is mainly characterized by symptoms such as burning, stinging, itching, and tightness after exposure to external stimuli, with or without symptoms like erythema, scaling, and telangiectasia. The primary cause is damage to the skin barrier and weakening of its function due to exogenous factors.

[0003] The human skin barrier is located in the outermost layer of the skin, the stratum corneum, which is composed of keratinocytes and lipids in the intercellular space. The integrity of the skin barrier function is mainly based on the composition of epidermal lipids. In sensitive skin, the intercellular lipids are mainly composed of fat-soluble cholesterol and fatty acids, acting as "cement" in the skin's brick-and-mortar structure. A lack of lipids leads to increased transepidermal water loss (TEWL), reduced stratum corneum hydration, and weakened skin barrier function, making the skin more susceptible to various irritants and causing sensitivity symptoms. However, with increased urban air pollution, high stress levels, irregular lifestyles, and the growing popularity of cosmetic procedures, the number of people with sensitive skin is increasing.

[0004] Chinese patent application 202110588884.7 discloses a soothing and redness-reducing anti-allergy face cream, comprising the following components: 10-40 parts water, 2-5 parts butylene glycol, 3-10 parts glycerin, 1-7 parts decapeptide-4, 0.1-2 parts yeast fermentation product, 0.3-1.2 parts peach resin extract, 0.2-1 part purslane extract, 0.5-4 parts betaine, etc. This formula is suitable for people with allergies, improves facial redness symptoms, and is gentle and non-irritating. It effectively treats and relieves induced redness without side effects. Moreover, the gentle formula can reduce skin dryness, moisturize the skin, keep the skin moist and refreshed, help the skin absorb nutrients, increase skin elasticity, and promote skin repair.

[0005] Further examination of the specification section of the plan reveals that although the plan discloses purslane extract and peach resin extract, it does not disclose the use of peony extract.

[0006] The problem this solution aims to solve is: how to develop a composition that differs from existing technologies and possesses excellent anti-allergic, soothing, and repairing abilities. Summary of the Invention

[0007] The purpose of this application is to provide a highly effective anti-allergic, soothing, and repairing composition. This composition further repairs the skin barrier through the interaction between purslane extract, peach resin extract, and peony extract, and exhibits a certain inhibitory ability on inflammatory factors.

[0008] To achieve the above objectives, this application discloses a highly effective anti-allergic, soothing, and repairing composition containing peony extract, peach resin extract, and purslane extract, wherein the mass ratio of peony extract, peach resin extract, and purslane extract is 0.008–2.4: 0.02–0.4: 0.000008–0.48.

[0009] Peony extract contains a variety of anti-inflammatory components that can help relieve skin inflammation, and is especially effective for people with sensitive skin or those with redness and swelling. At the same time, peony extract also has strong antioxidant properties, which can protect the skin from free radical damage, thereby slowing down skin aging.

[0010] Peach resin extract can inhibit skin inflammation and help reduce sensitivity and redness. In addition, peach resin extract can also relieve skin discomfort and soothe allergic reactions caused by external stimuli.

[0011] Purslane extract is rich in anti-inflammatory components, which can effectively reduce skin inflammation, relieve redness and irritation, and is especially effective for sensitive skin. It also has a good soothing effect, which can reduce skin discomfort caused by environmental irritants or allergies and help restore the skin to its natural state.

[0012] Preferably, the peony extract is extracted from the root of the peony plant, and the extraction method of the peony extract is as follows:

[0013] Step 1: Crush the peony root, then enzymatically hydrolyze it, inactivate the enzyme after hydrolysis, centrifuge and dry it to obtain the hydrolysis product;

[0014] Step 2: Boil the enzymatic hydrolysis product in water at 75-85℃ for 3-5 hours, then centrifuge to collect the supernatant to obtain peony extract.

[0015] Preferably, the enzyme in step 1 is a mixture of cellulase, pectin lyase and amylase, and the mass ratio of cellulase, pectin lyase and amylase is 4-6:2:1.

[0016] Preferably, the highly effective anti-allergic soothing and repairing composition also contains at least one of cactus extract, oat bran extract, peony root bark extract, and centella asiatica extract.

[0017] Preferably, the highly effective anti-allergic, soothing, and repairing composition also contains ceramide, and the mass ratio of ceramide to purslane extract is 0.000025-0.2:0.000008-0.48.

[0018] Preferably, the product comprises at least the following components by mass:

[0019] Peony extract 0.008–2.4 parts;

[0020] Peach resin extract, 0.02–0.4 parts;

[0021] Purslane extract 0.000008–0.48 parts;

[0022] Cactus extract, 0.00005–0.03 parts;

[0023] Oat bran extract, 0.00003–0.018 parts;

[0024] Peony root extract, 0.00002–0.012 parts;

[0025] Centella asiatica extract, 0.00001–0.006 parts;

[0026] Ceramide 0.000025 to 0.2 parts.

[0027] In addition, this application also discloses a method for preparing the above-mentioned highly effective anti-allergic soothing and repairing composition, wherein peony extract, peach resin extract and purslane extract are mixed to obtain the highly effective anti-allergic soothing and repairing composition.

[0028] In addition, this application also discloses the formulation and application of cosmetics prepared using the above-mentioned highly effective anti-allergic, soothing and repairing composition.

[0029] The beneficial effects of this application are:

[0030] Peony extract contains a variety of anti-inflammatory components that can help relieve skin inflammation, and is especially effective for people with sensitive skin or those with redness and swelling. At the same time, peony extract also has strong antioxidant properties, which can protect the skin from free radical damage, thereby slowing down skin aging.

[0031] Peach resin extract can inhibit skin inflammation and help reduce sensitivity and redness. In addition, peach resin extract can also relieve skin discomfort and soothe allergic reactions caused by external stimuli.

[0032] Purslane extract is rich in anti-inflammatory components, which can effectively reduce skin inflammation, relieve redness and irritation, and is especially effective for sensitive skin. It also has a good soothing effect, which can reduce skin discomfort caused by environmental irritants or allergies and help restore the skin to its natural state.

[0033] Furthermore, through the combination of the aforementioned ingredients, this application demonstrates that purslane extract can effectively reduce skin inflammation, peach resin extract can strengthen the skin barrier, and peony extract has a good relieving effect on sensitivity and redness. The healing ability of purslane extract can be combined with the cell regeneration properties of peony extract, while peach resin extract helps enhance the repair effect of the skin barrier. The combination of these three ingredients can more comprehensively promote the skin's self-repair, resulting in healthier skin. Detailed Implementation

[0034] The present invention will now be clearly and completely described in conjunction with embodiments thereof. It should be noted that, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0035] Before presenting the examples and comparative examples, the raw materials involved in the examples and comparative examples are described below as follows, as shown in Table 1:

[0036] Table 1: Raw Material Information Table

[0037] raw material supplier Product information (such as the content of active ingredients, the monomers and degree of polymerization of the polymer, the molecular weight of the substance, etc.) raw material supplier Product information (such as the content of active ingredients, the monomers and degree of polymerization of the polymer, the molecular weight of the substance, etc.) Peony extract Guangzhou Panhai Fine Chemical Co., Ltd. The effective substance content is 8%. cactus extract Guangzhou Snoke Biotechnology Co., Ltd. The effective substance content is 10%. Peach resin extract Guangzhou Bodalongshang Trading Co., Ltd. The effective substance content is 20%. Oat bran extract Guangzhou Shengrui Chemical Technology Co., Ltd. The effective substance content is 10%. Purslane extract Guangzhou Yimu New Material Technology Co., Ltd., Raw Material Name The effective substance content is 0.8%. Peony root bark extract Guangzhou Snoke Biotechnology Co., Ltd. The effective substance content is 10%. ceramide Guangzhou Panhai Fine Chemical Co., Ltd. The effective substance content is 2.5%. Centella Asiatica extract Guangzhou Snoke Biotechnology Co., Ltd. The effective substance content is 10%. Cellulase Hubei Supu Chemical Co., Ltd. The effective substance content is 99%. Pectin lyase Jiangsu Ruister Biotechnology Co., Ltd. The effective substance content is 99.9%. amylase Hubei Maoerwo Biomedical The effective substance content is 98%.

[0038] It should be noted that, unless otherwise specified, the extraction method of peony extract in the following examples and comparative examples is this method, specifically as follows:

[0039] Step 1: Crush the peony root, then add cellulase at a mass ratio of 10:0.1 of crushed peony root to enzyme and enzymatically hydrolyze for 12 hours. After enzymatic hydrolysis, inactivate the enzyme, centrifuge and dry to obtain the enzymatic hydrolysis product.

[0040] Step 2: Boil the enzymatic hydrolysate in water at 80±3℃ for 4 hours, then centrifuge to collect the supernatant to obtain peony extract.

[0041] The method for preparing the composition is as follows (it should be noted that, unless otherwise specified, this method is used in the following examples and comparative examples):

[0042] Peony extract, peach resin extract and purslane extract were mixed and dispersed at 500 rpm for 20 ± 3 min to obtain a highly effective anti-allergic, soothing and repairing composition.

[0043] (It should be noted that in the following examples and comparative examples, the amount of each raw material added is based on the mass fraction of the effective substance in the raw material.)

[0044] Examples 1-3

[0045] A highly effective anti-allergic, soothing, and repairing composition, the formulation of which is shown in Table 2:

[0046] Table 2: Formulation Tables for Examples 1-3

[0047] Group Peony extract Peach resin extract Purslane extract Example 1 0.048 copies 0.1 copies 0.0016 copies Example 2 0.008 copies 0.02 copies 0.000008 copies Example 3 2.4 copies 0.4 copies 0.48 copies

[0048] Example 4

[0049] The method is basically the same as in Example 1, except that, while keeping the mass ratio between plant extracts unchanged, commercially available peony extract is used to replace the peony extract prepared in Example 1.

[0050] Example 5

[0051] The method is basically the same as in Example 1, except that in the extraction process of peony extract, a mixture of pectin lyase and amylase is used to replace cellulase, and the mass ratio of pectin lyase to amylase is 2:1.

[0052] Example 6

[0053] The method is basically the same as in Example 1, except that in the extraction process of peony extract, a mixture of cellulase, pectin lyase and amylase is used to replace cellulase, and the mass ratio of cellulase, pectin lyase and amylase is 5:2:1.

[0054] Examples 7-12

[0055] A highly effective anti-allergic, soothing, and repairing composition, the formulation of which is shown in Table 3:

[0056] Table 3: Formulation Tables for Examples 7-12

[0057] Group Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Peony extract 0.048 copies 0.048 copies 0.048 copies 0.048 copies 0.048 copies 0.048 copies Peach resin extract 0.1 copies 0.1 copies 0.1 copies 0.1 copies 0.1 copies 0.1 copies Purslane extract 0.0016 copies 0.0016 copies 0.0016 copies 0.0016 copies 0.0016 copies 0.0016 copies cactus extract 0.005 copies 0 0 0.005 copies 0.00005 copies 0.03 copies Oat bran extract 0.003 copies 0 0 0.003 copies 0.00003 copies 0.018 copies Peony root extract 0 0.002 copies 0 0.002 copies 0.00002 copies 0.012 copies Centella Asiatica extract 0 0.001 copies 0 0.001 copies 0.006 copies 0.00001 copies ceramide 0 0 0.0025 copies 0.0025 copies 0.2 copies 0.000025 copies

[0058] Comparative Examples 1-6

[0059] A composition, the formulation of which is shown in Table 4:

[0060] Table 4: Formulation Tables for Comparative Examples 1-6

[0061] Group Peony extract Peach resin extract Purslane extract Comparative Example 1 0.048 copies 0 0 Comparative Example 2 0 0.1 copies 0 Comparative Example 3 0 0 0.0016 copies Comparative Example 4 0.048 copies 0.1 copies 0 Comparative Example 5 0.048 copies 0 0.0016 copies Comparative Example 6 0 0.1 copies 0.0016 copies

[0062] Performance testing

[0063] 1. Hyaluronidase inhibition rate

[0064] 1.1 The relationship between hyaluronidase and skin soothing

[0065] Hyaluronidase is an enzyme that cleaves hyaluronic acid and plays a crucial role in allergic reactions, strongly correlated with the release of substances such as histamine. The soothing effect of a test sample can be determined using the hyaluronidase inhibition rate; a higher inhibition rate indicates a stronger soothing effect.

[0066] 1.2 Treatment of control and test substances

[0067] Based on the mass of the active substance in the compositions prepared in the examples and comparative examples, the active substance in the compositions prepared in each example and comparative example was diluted with pure water to a mass fraction of 5% aqueous solution as the test substance; in addition, pure water was used as a negative control.

[0068] 1.3 Experimental Procedures

[0069] Set up a test substance group, a test substance background group, a solvent group, and a solvent background group. Each group should have 3 replicates. Add different reagent solutions to each of the four groups, shake well, and let stand at room temperature for 30 minutes to develop color. Measure the absorbance value at a wavelength of 528 nm using a UV spectrophotometer.

[0070] 1.4 The calculation formula is shown in Equation 3:

[0071] Formula 3

[0072] In the formula: A—is the absorbance of the reaction solution without sample and the reaction solution containing enzyme;

[0073] B—The absorbance of the reaction solution without the sample and without the enzyme;

[0074] C—is the absorbance of the sample and the reaction solution containing the enzyme;

[0075] D—The absorbance of the reaction solution containing the sample and the reaction solution without the enzyme.

[0076] The final test results are shown in Table 5:

[0077] Table 5: Results of Hyaluronidase Inhibition Rate Test

[0078] Group Hyaluronidase inhibition rate (%) Group Hyaluronidase inhibition rate (%) Example 1 33.846 Example 10 39.936 Example 2 31.019 Example 11 37.852 Example 3 32.923 Example 12 37.014 Example 4 29.125 Comparative Example 1 18.843 Example 5 32.722 Comparative Example 2 19.065 Example 6 36.893 Comparative Example 3 21.530 Example 7 35.640 Comparative Example 4 22.197 Example 8 32.815 Comparative Example 5 23.099 Example 9 34.808 Comparative Example 6 20.573 negative control 1.038

[0079] 2. Inflammatory factor inhibition rate test

[0080] Representative experimental groups from the hyaluronidase inhibition rate test were selected for further testing of inflammatory factor inhibition rate. Using the mass of the active ingredient in the composition as the standard, the active ingredient in the compositions obtained in Examples 1 and 10 was diluted with pure water to a 5% (w / w) aqueous solution; this was used as the test substance, and the determination was subsequently performed. The specific method is as follows:

[0081] Step 1: Inoculate with mouse mononuclear macrophage leukemia cell line RAW264.7: Dilute cells with cell culture medium to the inoculation density (confluence reaches 45% to 60% 24 h after inoculation), then inoculate into 96-well plates with 200 μL of liquid per well, and incubate in a CO2 incubator for 24 ± 2 h.

[0082] Step 2: Discard the culture medium in the 96-well plate and proceed with the drug administration. Add culture medium containing 1% of the test substance to the test substance wells, and add normal cell culture medium to the blank / solvent control wells, 200 μL per well. After drug administration, place the 96-well plate in a CO2 incubator and incubate for 24 h ± 2 h.

[0083] (3) Testing: After a period of culture, 200 μL of cell culture supernatant was collected in a 1.5 mL sterile centrifuge tube and stored at -80℃. The TNF-α ELISA kit was used for testing, and the results are shown in Table 6.

[0084] Table 6: Results of Inflammatory Factor Inhibition Rate Test

[0085] sample TNF-α sample TNF-α Example 1 -12.44% Example 10 -18.56%

[0086] Results analysis:

[0087] 1. As can be seen from Table 5, after adjusting the mass ratio between the raw materials in Examples 1-3, the hyaluronidase inhibition rate of Examples 1-3 fluctuated to some extent, but the amplitude was small. It can be seen that adjusting the mass ratio between the raw materials in the composition within a small range has a relatively small impact on the hyaluronidase of the composition.

[0088] 2. As can be seen from Examples 4-6, when the enzymes used in the extraction process of peony extract were adjusted, firstly, after replacing the self-made peony extract with commercially available peony extract, the hyaluronidase in Example 4 showed a more obvious decreasing trend compared to Example 1.

[0089] Furthermore, when cellulase was replaced with a mixture of pectin lyase and amylase, the hyaluronidase inhibition rate of the composition prepared in Example 5 showed a slight decreasing trend compared to Example 1. However, when the enzyme was adjusted to a mixture of cellulase, pectin lyase and amylase, Example 6 showed a more significant increasing trend compared to Examples 1 and 5. It can be seen that the peony extract obtained by enzymatic hydrolysis using the above enzymes may contain a richer content or variety of active substances.

[0090] 3. As can be seen from Examples 1 and 7-12, when other plant extracts such as cactus extract, oat bran extract, peony root extract, and centella asiatica extract are added to the composition; or when ceramide is added, Examples 7-9 all show different degrees of improvement compared to Example 1 (except for Example 8). However, further observation of Example 10 shows that when cactus extract, oat bran extract, peony root extract, centella asiatica extract and ceramide are added to Example 10 at the same time, its hyaluronidase inhibition rate shows a more significant improvement compared to Example 1, and the improvement of Example 10 exceeds the sum of the improvements of Examples 7-9 compared to Example 1.

[0091] 4. Observation of Comparative Examples 1-3 shows that when the composition contains only peony extract, peach resin extract, or purslane extract, the hyaluronic acid inhibition rate of Comparative Examples 1-3 is around 18-21%. Furthermore, when any two of the peony extract, peach resin extract, and purslane extract are used in combination, the hyaluronidase inhibition rate of Comparative Example 4 is higher than that of any one of Comparative Examples 1-2, and the hyaluronidase of Comparative Example 5 is also significantly higher than that of any one of Comparative Examples 1 and 3. It can be seen that there is a certain synergistic effect between peony extract and peach resin extract, and between peony extract and purslane extract.

[0092] However, further observation of Example 1 shows that when peony extract, peach resin extract and purslane extract are used simultaneously, the hyaluronidase content of the composition prepared in Example 1 is significantly higher than that of any of Comparative Examples 1-3, and its increase relative to Comparative Examples 1-3 is significantly higher than that of Comparative Examples 4-6 relative to Comparative Examples 1-3. It can be seen that when the herbal extracts, peach resin extract and purslane extract are used together, the synergistic effect among the three is further amplified, thereby further improving the hyaluronidase inhibition ability of Example 1.

[0093] As can be seen from Table 6, the compositions prepared in Examples 1 and 10 both have good inhibitory ability on inflammatory factors.

Claims

1. A highly effective anti-allergic, soothing, and repairing composition, characterized in that, It contains peony extract, peach resin extract and purslane extract, wherein the mass ratio of peony extract, peach resin extract and purslane extract is 0.008-2.4:0.02-0.4:0.000008-0.48; The peony extract is extracted from the root of the peony plant, and the extraction method of the peony extract is as follows: Step 1: Crush the peony root, then enzymatically hydrolyze it, inactivate the enzyme after hydrolysis, centrifuge and dry it to obtain the hydrolysis product; Step 2: Boil the enzymatic hydrolysis product in water at 75-85℃ for 3-5 hours, then centrifuge to collect the supernatant to obtain peony extract.

2. The highly effective anti-allergic, soothing, and repairing composition according to claim 1, characterized in that, The enzyme in step 1 is a mixture of cellulase, pectin lyase and amylase, and the mass ratio of cellulase, pectin lyase and amylase is 4-6:2:

1.

3. The highly effective anti-allergic, soothing, and repairing composition according to claim 1, characterized in that, The highly effective anti-allergic, soothing, and repairing composition also contains at least one of the following: cactus extract, oat bran extract, peony root bark extract, and centella asiatica extract.

4. The highly effective anti-allergic, soothing, and repairing composition according to claim 1, characterized in that, The highly effective anti-allergic, soothing, and repairing composition also contains ceramides, and the mass ratio of ceramides to purslane extract is 0.000025–0.2:0.000008–0.

48.

5. The highly effective anti-allergic, soothing, and repairing composition according to claim 1, characterized in that, By weight, it shall include at least the following components: Peony extract 0.008–2.4 parts; Peach resin extract, 0.02–0.4 parts; Purslane extract 0.000008–0.48 parts; Cactus extract, 0.00005–0.03 parts; Oat bran extract, 0.00003–0.018 parts; Peony root extract, 0.00002–0.012 parts; Centella asiatica extract, 0.00001–0.006 parts; Ceramide 0.000025 to 0.2 parts.

6. A method for preparing the highly effective anti-allergic, soothing, and repairing composition according to any one of claims 1-5, characterized in that, A highly effective anti-allergic, soothing, and repairing composition was obtained by mixing peony extract, peach resin extract, and purslane extract.

7. The use of the highly effective anti-allergic, soothing, and repairing composition according to any one of claims 1-5 in the preparation of cosmetics.