Lactobacillus paracasei subsp. Immunitas free exopolysaccharide with immunostimulatory activity, process for its preparation and use

Abstract

This invention relates to the field of microbial technology, and particularly to a free extracellular polysaccharide of *Lactobacillus paracasei* with immunostimulatory activity, its preparation, and its application. This invention provides an immunostimulatory extracellular polysaccharide of *Lactobacillus paracasei*, which is a free extracellular polysaccharide with a molecular weight of approximately 7.66 × 10⁻⁶. 5 Da contains the following monosaccharides: glucose content of 98.90%, glucuronic acid content of 0.61%, and mannose content of 0.49%. The main chain is composed of (1→4)-α-D-glucopyranosyl groups, and the side chains are composed of (1→6)-α-D-glucopyranosyl groups and (1→3)-α-D-glucopyranosyl groups, making it a novel polysaccharide. It promotes the proliferation and secretion of IL-1β, TNF-α, IL-6, and IL-10 by RAW264.7 cells. Furthermore, this invention provides a method for preparing this polysaccharide, which produces and achieves high yields, laying the foundation for its application.

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