Mussel mucoprotein and hyaluronic acid cross-linked self-repairing eye health-care cream and 4D forming method thereof

Abstract

The invention relates to the technical field of biological medicine, in particular to mussel mucin and hyaluronic acid cross-linked self-repairing eye health care cream and a 4D forming method thereof. The composition comprises stearic acid, cetostearyl alcohol and the like, a lactic acid bacteria fermentation extract, a jasmine flower extract, a wolfberry extract, a pseudo-ginseng extract, a cassia seed extract, a polygonum cuspidatum extract, a chrysanthemum extract, a leontopodium leontopodum extract, ethylparaben, methylparaben and the like, decapeptide-4, mussel mucin, proteoglycan protein and modified graphene. Modified graphene in the self-repairing eye health care cream can effectively promote crosslinking of mussel mucin and hyaluronic acid and improve the repairing effect, the micro-current effect of graphene can also promote skin tissue to absorb traditional Chinese medicine components and deeply repair eyes, the effect can be more obvious according to personal eye conditions and requirements, and the self-repairing eye health care cream has a good application prospect. The composition has the effects of relieving asthenopia, dryness and soreness, effectively delaying excessive growth of an ocular axis, relieving temporary myopia drift, relieving asthenopia and protecting near vision.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid and a 4D molding method thereof. Background Art

[0002] With the rapid development of technology, electronic products have become an integral part of our daily lives. Smartphones, tablets, laptops, and various screens dominate our work, study, and entertainment. However, this reliance on electronic devices has also led to a series of eye health problems, the prevalence and severity of which are increasingly gaining attention. Staring at a screen for extended periods, especially in low-light environments, can cause eye fatigue and impair vision. This vision loss is often gradual and, if left unchecked, can lead to permanent vision damage.

[0003] Radiation such as blue light emitted by electronic products is potentially harmful to the eyes. Long-term exposure to this radiation may increase the risk of eye diseases such as macular degeneration.

[0004] The use of electronic products causes the eye muscles to be in a tense state for a long time, accelerating the aging of the eye skin and making problems such as eye wrinkles, dark circles and bags under the eyes more obvious.

[0005] Although some self-repairing eye care creams are available on the market, which can relieve eye fatigue and improve eye skin conditions to a certain extent, the current self-repairing eye care creams still have shortcomings:

[0006] Many eye ointments rely solely on pharmaceutical ingredients to work, but these ingredients often only address surface issues and fail to penetrate deep into eye tissue for repair. While ointments may provide some short-term relief, their effectiveness diminishes with long-term use, rendering them ineffective for sustained eye care. Individuals vary in their eye conditions and responses to medications, resulting in some ointments being effective for some but less effective for others. Summary of the Invention

[0007] One of the purposes of the present application is to provide a self-repairing eye care cream with mussel adhesive protein and hyaluronic acid cross-linking to avoid the shortcomings in the prior art, which can deeply repair the eyes and provide personalized eye care effects according to individual eye conditions and needs, thereby improving the applicability and effects of the product. Specifically, the self-repairing eye care cream combines various traditional Chinese medicine ingredients and uses modified graphene as an auxiliary effect. The modified graphene can effectively promote the cross-linking of mussel adhesive protein and hyaluronic acid, so that the various traditional Chinese medicine extracts can be stably and tightly applied to the eye position, improve the repair effect, and the micro-current effect of graphene can also promote the skin tissue to absorb the traditional Chinese medicine ingredients, which can deeply repair the eyes and provide personalized eye care effects according to individual eye conditions and needs. On this basis, the formula can reduce the accumulation of lactic acid caused by continuous contraction of the ciliary muscle, thereby quickly relieving visual fatigue, dryness and acid swelling. At the same time, heating the eye area at 39-41℃ can accelerate local microcirculation, effectively delay the excessive growth of the eye axis, reduce the temporary myopia drift caused by close-range eye use, and individualize the relief of visual fatigue and myopia protection effects through the paste application time or frequency according to the intensity of eye use.

[0008] The second purpose of the present application is to provide a preparation method of a self-repairing eye care cream with mussel adhesive protein and hyaluronic acid cross-linking to avoid the shortcomings in the prior art.

[0009] The third purpose of the present application is to provide a 4D forming method of a self-repairing eye care cream with mussel adhesive protein and hyaluronic acid cross-linking, which can effectively improve the efficacy of the eye care cream by combining the 4D method.

[0010] To achieve one of the above purposes, the present application provides the following technical solutions:

[0011] The self-repairing eye care cream with mussel adhesive protein and hyaluronic acid cross-linking is provided, which comprises the following components by weight,

[0012] stearic acid 1-5 parts, cetearyl alcohol 1-3 parts, distilled monostearin 1-3 parts, vaseline 5-10 parts

[0013] butanediol 1-5 parts, menthol 0.1-1 part, borneol 0.1-1 part, lactic acid bacteria fermentation extract 1-5 parts, jasmine extract 1-3 parts, medlar extract 1-3 parts, panax notoginseng extract 1-3 parts, cassia seed extract 1-3 parts, giant knotweed extract 1-3 parts, chrysanthemum extract 1-3 parts, leontopodium leontopodioides extract 1-3 parts, nipagin ethyl ester 0.01-0.1 part, nipagin methyl ester 0.01-0.1 part, oat extract 1-5 parts, decapeptide-4 0.01-0.1 part, mussel adhesive protein 0.1-1 part, polysaccharide protein 0.5-2 parts, modified graphene 0.01-0.1 part, and the balance is pure water.

[0014] The modified graphene is prepared by the following method:

[0015] Step 1: Activate hyaluronic acid:

[0016] The formulated amount of hyaluronic acid is dissolved in PBS buffer, and the mass-to-volume ratio of the hyaluronic acid to PBS is: 1-2 mg: 8-10 mL;

[0017] Adding formulated amounts of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide to a hyaluronic acid solution, wherein the molar ratio of N-hydroxysuccinimide to 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide is 1-2:1-2;

[0018] Stirring at room temperature for 1 to 2 hours is to activate the carboxyl groups on the hyaluronic acid. After the reaction time is completed, adding ethanolamine to the first mixed solution in an amount of 0.2% to 0.5% of the first mixed solution to neutralize unreacted N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide to obtain activated hyaluronic acid;

[0019] Step 2:

[0020] The graphene of the formula amount is dispersed in PBS buffer solution, wherein the mass to volume ratio of the graphene to the PBS buffer solution is: 2mg-3mg:10ml-20ml, to obtain a graphene dispersion liquid, and the activated hyaluronic acid solution and the graphene dispersion liquid are mixed, wherein the weight ratio of the activated hyaluronic acid solution to the graphene dispersion liquid is 1-2:10-1,

[0021] The pH of the graphene dispersion was adjusted to neutral using PBS buffer, and the reaction was monitored by UV-visible spectroscopy until the hyaluronic acid and graphene were covalently bonded to obtain modified graphene.

[0022] In some embodiments, the modified graphene is purified by:

[0023] The modified graphene was placed in a dialysis bag and dialyzed in PBS buffer to remove small molecule byproducts and unreacted hyaluronic acid. The dialysis time was 12h to 24h.

[0024] The mixture was then centrifuged at 10,000 to 15,000 rpm for 8 to 15 minutes, and the supernatant was collected.

[0025] Provided is a method for preparing a self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid, comprising the following steps:

[0026] Oil phase preparation:

[0027] Mix stearic acid, cetostearyl alcohol, distilled glyceryl monostearate and vaseline in a container, and heat the mixture to 60-70°C to keep the oil phase in liquid form;

[0028] Aqueous phase preparation:

[0029] Add butylene glycol, ethylparaben, methylparaben, oat extract, decapeptide-4, mussel mucin and polysaccharide protein to purified water, heat to 40°C to 50°C, detect and adjust the pH of the aqueous phase to 6-7, and maintain the aqueous phase state;

[0030] Extract processing:

[0031] Dilute menthol, borneol, lactic acid bacteria fermentation extract, jasmine extract, wolfberry extract, Panax notoginseng extract, cassia seed extract, knotweed extract, chrysanthemum extract and leontopodium edulis extract in pure water respectively, so that each extract is completely dissolved;

[0032] Emulsification process:

[0033] Before starting emulsification, the oil phase and the water phase are heated to 40°C to 60°C respectively, and the temperature is maintained constant. The heated oil phase is slowly added to the water phase to perform a pre-mixing stage. The stirring speed of the pre-mixing stage is 100 rpm to 300 rpm, and then the stirring speed is 1000 rpm to 10000 rpm to ensure sufficient and uniform mixing. During the emulsification process, the pH is regularly monitored and adjusted to 6 to 7 to obtain an emulsion;

[0034] Add modified graphene:

[0035] Disperse the modified graphene in purified water to form a uniform suspension, slowly add the suspension to the emulsion, and continue stirring until a paste is obtained;

[0036] During the emulsification process, a formulated amount of crown ether is also added.

[0037] In some embodiments, the weight ratio of the crown ether to the emulsion is 1:8-10.

[0038] In some embodiments, the crown ether is any one of a tetraalkylammonium salt, a crown sulfide, or a tetraphenylphosphine.

[0039] In some embodiments, after emulsification is completed, the crown ether is dissolved in butanediol and then slowly added to the cooled emulsion or paste with sufficient stirring.

[0040] In some embodiments, the cooling temperature is 8-10° C., and the stirring speed is 100 rpm-150 rpm.

[0041] A 4D molding method for a self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid, using the self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid prepared above, is applied to a massage stick, the massage stick including a massage head, the massage head consisting of an electromagnet, the electromagnet being connected to an electric current, the self-repairing eye care cream being applied to the eye through the massage head, when in use, the massage head is connected to an electric current, the magnetic field generated by the electromagnet stimulates the self-repairing eye care cream applied to the eye and causes the cross-linking of hyaluronic acid and mussel mucin through the conductive effect of graphene.

[0042] In some embodiments, the surface of the massage head that contacts the eyes is designed to have honeycomb grooves, and the depth of each groove is 2 mm to 5 mm.

[0043] In some embodiments, the current is 50 to 80 microamperes.

[0044] The beneficial effects of the self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid of the present invention are as follows:

[0045] (1) In the present invention, hyaluronic acid is a polysaccharide naturally present in the human body, which has excellent moisturizing and lubricating effects and can provide sufficient moisture and nutrition for the skin around the eyes. Combined with the strong adhesion and biocompatibility of mussel mucin, the repair and protective effects of the eye care cream can be enhanced. Hyaluronic acid is repaired on graphene with conductive network lines, so that the eye care cream can not only provide traditional moisturizing and repair functions, but also stimulate the microcirculation around the eyes through the conductive properties of graphene, promote metabolism, and enhance the vitality of the skin around the eyes. The conductive function of graphene can serve as a physical stimulus to promote the cross-linking between mussel mucin and hyaluronic acid, forming a more stable gel network, and improving the self-repair ability and durability of the eye care cream.

[0046] Furthermore, by adjusting the amount of graphene used, the conductivity and repair effects can be customized to suit the characteristics and needs of the eye area at different ages, achieving a personalized eye care and repair plan. Young skin requires less graphene to avoid overirritation, while mature skin requires more to enhance the repair effect. The cross-linked structure of mussel mucin and hyaluronic acid forms a protective film on the surface of the eye area. This film not only locks in moisture but also promotes self-repair of skin cells, reducing the appearance of fine lines and wrinkles. Graphene's conductive properties help activate cells in the eye area and increase their vitality, thereby combating signs of aging, such as dark circles and eye bags. Since mussel mucin and hyaluronic acid are both highly biocompatible materials, combined with the biosafety of graphene, this eye care ointment not only provides efficient health care effects, but also ensures safety and comfort of use, achieving more significant relief of visual fatigue and near vision protection effects. It is suitable for problems such as excessive eye strain, blurred vision, myopia, astigmatism and amblyopia, and can achieve personalized intervention in visual fatigue in multiple scenarios such as myopia, astigmatism, amblyopia and blurred vision, which is beneficial to eye health and vision improvement.

[0047] (2) In the present invention, menthol (0.1-1 parts) has a cooling effect and can provide an immediate soothing effect, and is often used to relieve eye fatigue; borneol (0.1-1 parts) has a cooling effect and helps relieve eye discomfort and fatigue. Lactic acid bacteria fermentation extract (1-5 parts) contains a variety of beneficial ingredients, which are helpful for skin health and repair; jasmine extract (1-3 parts) has antioxidant and soothing effects and helps protect the skin; wolfberry extract (1-3 parts) is rich in antioxidants and helps protect the skin from free radical damage; Panax notoginseng extract (1-3 parts) has anti-inflammatory and blood circulation promoting effects; Cassia seed extract (1-3 parts) helps improve eye blood circulation and relieve eye fatigue; Polygonum cuspidatum extract (1-3 parts) has anti-inflammatory and antioxidant effects; Chrysanthemum extract (1-3 parts) has soothing and antioxidant effects; Leontopodium edelweiss extract (1-3 parts) helps soothe the skin and reduce irritation; Oat extract (1-5 parts) has soothing and moisturizing effects and helps protect the skin. Decapeptide-4 (0.01-0.1 parts) is a peptide ingredient that helps promote collagen production and reduce wrinkles. Mussel mucin (0.1-1 parts) has strong adhesion and biocompatibility, helping to form a protective film and promote skin repair. Polysaccharide protein (0.5-2 parts) helps moisturize and provide nutrition to promote skin health.

[0048] To achieve the second of the above objectives, the present invention provides the following technical solutions:

[0049] A self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid is provided, comprising the following components in parts by weight:

[0050] 1-5 parts stearic acid, 1-3 parts cetostearyl alcohol, 1-3 parts distilled monostearate glyceryl, 5-10 parts petrolatum

[0051] 1-5 parts of butylene glycol, 0.1-1 part of menthol, 0.1-1 part of borneol, 1-5 parts of lactic acid bacteria fermented extract, 1-3 parts of jasmine extract, 1-3 parts of wolfberry extract, 1-3 parts of Panax notoginseng extract, 1-3 parts of cassia seed extract, 1-3 parts of knotweed extract, 1-3 parts of chrysanthemum extract, 1-3 parts of leontopodium edodes extract, 0.01-0.1 part of ethylparaben, 0.01-0.1 part of methylparaben, 1-5 parts of oat extract, 0.01-0.1 part of decapeptide-4, 0.1-1 part of mussel mucin, 0.5-2 parts of polysaccharide protein, 0.01-0.1 part of modified graphene, and the balance is pure water;

[0052] The modified graphene is prepared by the following method:

[0053] Step 1: Activate hyaluronic acid:

[0054] The formulated amount of hyaluronic acid is dissolved in PBS buffer, and the mass-to-volume ratio of the hyaluronic acid to PBS is: 1-2 mg: 8-10 mL;

[0055] Adding formulated amounts of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide to a hyaluronic acid solution, wherein the molar ratio of N-hydroxysuccinimide to 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide is 1-2:1-2;

[0056] Stirring at room temperature for 1 to 2 hours is to activate the carboxyl groups on the hyaluronic acid. After the reaction time is completed, adding ethanolamine to the first mixed solution in an amount of 0.2% to 0.5% of the first mixed solution to neutralize unreacted N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide to obtain activated hyaluronic acid;

[0057] Step 2:

[0058] The graphene of the formula amount is dispersed in PBS buffer solution, wherein the mass to volume ratio of the graphene to the PBS buffer solution is: 2mg-3mg:10ml-20ml, to obtain a graphene dispersion liquid, and the activated hyaluronic acid solution and the graphene dispersion liquid are mixed, wherein the weight ratio of the activated hyaluronic acid solution to the graphene dispersion liquid is 1-2:10-1,

[0059] The pH of the graphene dispersion was adjusted to neutral using PBS buffer, and the reaction was monitored by UV-visible spectroscopy until the hyaluronic acid and graphene were covalently bonded to obtain modified graphene.

[0060] In some embodiments, the modified graphene is purified by:

[0061] The modified graphene was placed in a dialysis bag and dialyzed in PBS buffer to remove small molecule byproducts and unreacted hyaluronic acid. The dialysis time was 12h to 24h.

[0062] The mixture was then centrifuged at 10,000 to 15,000 rpm for 8 to 15 minutes, and the supernatant was collected.

[0063] Provided is a method for preparing a self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid, comprising the following steps:

[0064] Oil phase preparation:

[0065] Mix stearic acid, cetostearyl alcohol, distilled glyceryl monostearate and vaseline in a container, and heat the mixture to 60-70°C to keep the oil phase in liquid form;

[0066] Aqueous phase preparation:

[0067] Add butylene glycol, ethylparaben, methylparaben, oat extract, decapeptide-4, mussel mucin and polysaccharide protein to purified water, heat to 40°C to 50°C, detect and adjust the pH of the aqueous phase to 6-7, and maintain the aqueous phase state;

[0068] Extract processing:

[0069] Dilute menthol, borneol, lactic acid bacteria fermentation extract, jasmine extract, wolfberry extract, Panax notoginseng extract, cassia seed extract, knotweed extract, chrysanthemum extract and leontopodium edulis extract in pure water respectively, so that each extract is completely dissolved;

[0070] Emulsification process:

[0071] Before starting emulsification, the oil phase and the water phase are heated to 40°C to 60°C respectively, and the temperature is maintained constant. The heated oil phase is slowly added to the water phase to perform a pre-mixing stage. The stirring speed of the pre-mixing stage is 100 rpm to 300 rpm, and then the stirring speed is 1000 rpm to 10000 rpm to ensure sufficient and uniform mixing. During the emulsification process, the pH is regularly monitored and adjusted to 6 to 7 to obtain an emulsion;

[0072] Add modified graphene:

[0073] Disperse the modified graphene in purified water to form a uniform suspension, slowly add the suspension to the emulsion, and continue stirring until a paste is obtained;

[0074] During the emulsification process, a formulated amount of crown ether is also added.

[0075] In some embodiments, the weight ratio of the crown ether to the emulsion is 1:8-10.

[0076] In some embodiments, the crown ether is any one of a tetraalkylammonium salt, a crown sulfide, or a tetraphenylphosphine.

[0077] In some embodiments, after emulsification is completed, the crown ether is dissolved in butanediol and then slowly added to the cooled emulsion or paste with sufficient stirring.

[0078] In some embodiments, the cooling temperature is 8-10° C., and the stirring speed is 100 rpm-150 rpm.

[0079] A 4D molding method for a self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid, using the self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid prepared above, is applied to a massage stick, the massage stick including a massage head, the massage head consisting of an electromagnet, the electromagnet being connected to an electric current, the self-repairing eye care cream being applied to the eye through the massage head, when in use, the massage head is connected to an electric current, the magnetic field generated by the electromagnet stimulates the self-repairing eye care cream applied to the eye and causes the cross-linking of hyaluronic acid and mussel mucin through the conductive effect of graphene.

[0080] In some embodiments, the surface of the massage head that contacts the eyes is designed to have honeycomb grooves, and the depth of each groove is 2 mm to 5 mm.

[0081] In some embodiments, the current is 50 to 80 microamperes.

[0082] Beneficial effects of the preparation method of the self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid of the present invention:

[0083] The method for preparing the self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid of the present invention comprises preparing an oil phase and an aqueous phase separately, and then emulsifying the oil phase and the aqueous phase. After emulsification, we specifically add a crown ether, which is a macrocyclic compound containing multiple oxygen atoms. The crown ether enables it to form a stable complex with graphene particles, and the crown ether helps to evenly distribute the graphene in the emulsion. The evenly distributed graphene can better play its role in the eye care ointment, such as anti-oxidation, anti-inflammatory and promoting skin repair. The addition of the crown ether improves the stability of the emulsion, which is crucial for ensuring that the product maintains its performance during storage and use. The complex of the crown ether and graphene may enhance the functionality of the graphene and improve the health benefits of the self-repairing eye care ointment.

[0084] To achieve the third of the above objectives, the present invention provides the following technical solutions:

[0085] A 4D molding method for the self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid is provided. The self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid prepared above is used to apply the self-repairing eye care cream to a massage stick. The massage stick includes a massage head, and the massage head is composed of an electromagnet connected to an electric current. The self-repairing eye care cream is applied to the eye through the massage head. During use, the massage head is connected to the electric current, and the magnetic field generated by the electromagnet stimulates the self-repairing eye care cream applied to the eye and cross-links the hyaluronic acid and mussel mucin through the conductive effect of graphene.

[0086] In some embodiments, the surface of the massage head that contacts the eyes is designed to have honeycomb grooves, and the depth of each groove is 2 mm to 5 mm.

[0087] In some embodiments, the current is 50 to 80 microamperes.

[0088] Beneficial effects of the 4D molding method of the self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid of the present invention:

[0089] In the present invention, the self-repairing eye care cream contains graphene, which has a high magnetic permeability. When used, under the action of the magnetic field of the massage stick, the electrons in the graphene are affected by the Lorentz force, affecting the movement trajectory of the graphene, thereby improving the cross-linking efficiency of hyaluronic acid and mussel mucin. At the same time, the thermoelectric properties of graphene are improved in the magnetic field, thereby achieving auxiliary repair of the skin. DETAILED DESCRIPTION

[0090] The preferred embodiments of the present invention will be described in more detail below. Although preferred embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided to make the present invention more thorough and complete and to fully convey the scope of the present invention to those skilled in the art.

[0091] The terms used in this invention are for the purpose of describing specific embodiments only and are not intended to limit the invention. As used in this invention and the appended claims, the singular forms "a" and "the" are intended to include plural forms unless the context clearly indicates otherwise. It should also be understood that the term "and / or" as used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0092] It should be understood that although the terms "first", "second", "third", etc. may be used to describe various information in the present invention, such information should not be limited to these terms. These terms are only used to distinguish information of the same type from each other. For example, without departing from the scope of the present invention, the first information may also be referred to as the second information, and similarly, the second information may also be referred to as the first information. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, "plurality" means two or more, unless otherwise clearly and specifically defined.

[0093] Example 1

[0094] The self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid disclosed in this embodiment comprises the following components in parts by weight:

[0095] 1-5 parts of stearic acid, 1-3 parts of cetostearyl alcohol, 1-3 parts of distilled monostearate glyceryl, 5-10 parts of petrolatum, 1-5 parts of butylene glycol, 0.1-1 part of menthol, 0.1-1 part of borneol, 1-5 parts of lactic acid bacteria fermentation extract, 1-3 parts of jasmine extract, 1-3 parts of wolfberry extract, 1-3 parts of Panax notoginseng extract, 1-3 parts of cassia seed extract, 1-3 parts of knotweed extract, 1-3 parts of chrysanthemum extract, 1-3 parts of leontopodium cuspidatum extract, 0.01-0.1 part of ethylparaben, 0.01-0.1 part of methylparaben, 1-5 parts of oat extract, 0.01-0.1 part of decapeptide-4, 0.1-1 part of mussel mucin, 0.5-2 parts of polysaccharide protein, 0.01-0.1 part of modified graphene, and the balance is pure water;

[0096] The modified graphene is prepared by the following method:

[0097] Step 1: Activate hyaluronic acid:

[0098] The formulated amount of hyaluronic acid is dissolved in PBS buffer, and the mass-to-volume ratio of the hyaluronic acid to PBS is: 1-2 mg: 8-10 mL;

[0099] PBS buffer is a phosphate buffer with a pH of 7.4. Hyaluronic acid is dissolved in the PBS buffer to disperse the hyaluronic acid.

[0100] The mass-to-volume ratio of the hyaluronic acid to PBS is 1 mg:8 mL, or 1.5 mg:9 mL, or 2 mg:10 mL, which can be selected according to actual conditions.

[0101] Adding formulated amounts of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide to a hyaluronic acid solution, wherein the molar ratio of N-hydroxysuccinimide to 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide is 1-2:1-2;

[0102] N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide are used to activate the carboxyl groups on hyaluronic acid.

[0103] Specifically, hyaluronic acid is a polysaccharide with moisturizing and lubricating properties. Adding N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide (EDC) to the hyaluronic acid solution activates the carboxyl groups on the hyaluronic acid for further chemical modification or coupling reactions.

[0104] EDC is a zero-valent carboxylic acid activator that reacts with carboxylic acids to form active esters of carboxylic acids. Add EDC to the hyaluronic acid solution and allow the reaction to proceed at room temperature for a period of time to ensure that the carboxylic acid is fully activated. The chemical formula is:

[0105] HA-COOH+EDC→HA-COO-NH-CO-NH-CH2-CH2-N(CH3)2

[0106] NHS can further stabilize EDC-activated carboxylic acids to form NHS esters, which are more active intermediates and more easily react with amine compounds.

[0107] HA-COO-NH-CO-NH-CH2-CH2-N(CH3)2+NHS→HA-COO-NH-CO-NH-CH2-CH2-N(CH3)2-NH-CO-NH-S

[0108] Stirring at room temperature for 1 to 2 hours to activate the carboxyl groups on the hyaluronic acid, and after the reaction time is completed, adding ethanolamine to the first mixed solution in an amount of 0.2% to 0.5% of the first mixed solution to neutralize unreacted N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide to obtain activated hyaluronic acid;

[0109] Ethanolamine can neutralize unreacted N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide. This is because unreacted activator may interfere with subsequent coupling reactions or be toxic to biological systems.

[0110] Step 2:

[0111] The formula amount of graphene is dispersed in PBS buffer, and the mass and volume ratio of the graphene and the PBS buffer is 2 mg-3 mg: 10 ml-20 ml, the mass and volume ratio of the graphene and the PBS buffer is 2 mg: 10 ml, or the mass and volume ratio of the graphene and the PBS buffer is 3 mg: 20 ml, or the mass and volume ratio of the graphene and the PBS buffer is 2.5 mg: 15 ml, to obtain a graphene dispersion liquid, and the activated hyaluronic acid solution is mixed with the graphene dispersion liquid, and the weight ratio of the activated hyaluronic acid solution and the graphene dispersion liquid is 1-10: 1: 1, the carboxyl group of the activated hyaluronic acid reacts with the amine group on the graphene or reacts through other functional groups (such as hydroxyl, carboxyl, etc.) to form a covalent bond or a non-covalent interaction, thereby realizing the combination of hyaluronic acid and graphene.

[0112] The weight ratio of the activated hyaluronic acid solution and the graphene dispersion liquid is 1:1, or the weight ratio of the activated hyaluronic acid solution and the graphene dispersion liquid is 10:1, or the weight ratio of the activated hyaluronic acid solution and the graphene dispersion liquid is 8:1, which can be selected according to actual conditions.

[0113] The pH of the graphene dispersion liquid is adjusted to neutral using PBS buffer, and the reaction is monitored by ultraviolet-visible spectroscopy until the covalent combination of hyaluronic acid and graphene is obtained, thereby obtaining modified graphene.

[0114] Specifically, the dispersibility of graphene in water is poor and easy to aggregate, and adjusting the pH to neutral can reduce the electrostatic repulsion between graphene sheets, thereby improving the dispersion stability of graphene in water.

[0115] The pH value of PBS is close to the physiological environment of human body (pH 7.4), and adjusting the pH using PBS can ensure that the graphene dispersion liquid has good biocompatibility, which is crucial for subsequent biomedical applications. Under neutral conditions, the reactivity of functional groups (such as hydroxyl, carboxyl, etc.) on graphene is high, which is conducive to coupling reaction with biological macromolecules such as hyaluronic acid. Under acidic or alkaline conditions, the functional groups on graphene may undergo further chemical reactions, resulting in the generation of by-products. Adjusting the pH to neutral can reduce these side reactions and improve the purity and stability of the product.

[0116] In this embodiment, the modified graphene is purified by the following method:

[0117] The modified graphene is loaded into a dialysis bag and placed in PBS buffer for dialysis to remove small molecular by-products and unreacted hyaluronic acid, and the dialysis time is 12 h-24 h,

[0118] The mixture is then centrifuged at 10,000 rpm to 15,000 rpm for 8 to 15 minutes, and the supernatant is collected. The supernatant is the modified graphene.

[0119] Furthermore, the supernatant can be filtered using a 0.22 μm filter membrane to remove bacteria and particulate matter.

[0120] In this embodiment, the following steps are included:

[0121] Oil phase preparation:

[0122] Mix stearic acid, cetostearyl alcohol, distilled glyceryl monostearate and vaseline in a container, and heat the mixture to 60° C., 70° C., or 50° C., whichever is greater, to promote uniform mixing of the ingredients and keep the oil phase in a liquid state;

[0123] Aqueous phase preparation:

[0124] Add butylene glycol, ethylparaben, methylparaben, oat extract, decapeptide-4, mussel mucin and polysaccharide protein to purified water, heat to 40° C., 50° C., or 45° C., detect and adjust the pH of the aqueous phase to 6-7, and maintain the aqueous phase state;

[0125] Extract processing:

[0126] Dilute menthol, borneol, lactic acid bacteria fermentation extract, jasmine extract, wolfberry extract, Panax notoginseng extract, cassia seed extract, knotweed extract, chrysanthemum extract and leontopodium edulis extract in pure water respectively, so that each extract is completely dissolved;

[0127] The above-mentioned menthol, borneol, lactic acid bacteria fermentation extract, jasmine extract, wolfberry extract, Panax notoginseng extract, cassia seed extract, knotweed extract, chrysanthemum extract and leontopodium glutamate extract can be obtained on the market or obtained by extraction. The specific extraction methods are basically the same, specifically solvent extraction, water extraction, alcohol extraction, hydroalcohol extraction, distillation extraction, steam distillation, azeotropic distillation, supercritical fluid extraction (SFE), supercritical CO2 extraction, microwave-assisted extraction (MAE), using microwave heating to accelerate the interaction between solvent and medicinal materials and improve extraction efficiency, ultrasonic-assisted extraction (UAE), enzymatic extraction, membrane separation technology, and the specific selection can be made according to actual conditions.

[0128] Emulsification process:

[0129] Before starting emulsification, the oil phase and the water phase are heated to 40-60℃ respectively, and the temperature is kept constant. The heated oil phase is slowly added to the water phase, and the pre-mixing stage is carried out. The stirring speed in the pre-mixing stage is 100-300 rpm. First, low-speed stirring is carried out to avoid excessive shearing and foam generation. Low-speed stirring is helpful for the uniform dispersion of emulsifiers and active ingredients, and lays a foundation for subsequent emulsification. Then, stirring is carried out at a speed of 1000-10000 rpm to achieve sufficient and uniform mixing. During the emulsification process, the pH is regularly monitored and adjusted to 6-7 to ensure the stability of the system, and an emulsion is obtained.

[0130] Add modified graphene:

[0131] The modified graphene is dispersed in purified water to form a uniform suspension, and the suspension is slowly added to the emulsion, and stirring is continued until a paste is obtained.

[0132] After emulsification, the formula amount of crown ether is also added.

[0133] As a phase transfer catalyst, crown ether can form a stable complex with graphene ions for phase transfer of graphene.

[0134] In this embodiment, the weight ratio of the crown ether to the emulsion is 1:8, or the weight ratio of the crown ether to the emulsion is 1:10, or the weight ratio of the crown ether to the emulsion is 1:9.

[0135] In this embodiment, the crown ether is any one of a tetraalkylammonium salt, a crown thioether, or a tetraphenylphosphonium.

[0136] These four types of crown ethers are suitable for use in cosmetics. The tetraalkylammonium salt crown ether contains four alkylammonium salt groups, which form an ionic pair complex with metal ions. The cyclic structure of the crown thioether contains sulfur atoms instead of oxygen atoms. The introduction of sulfur atoms can change the electronic properties and coordination ability of the crown ether. The tetraphenylphosphonium crown ether contains four phenylphosphine groups, which can form a complex with metal ions. The tetraphenylphosphonium crown ether has a wide range of applications in metal organic chemistry due to its strong coordination ability and stability.

[0137] In this embodiment, after the emulsification is completed, the crown ether is dissolved in butanediol, and then slowly added to the cooled emulsion or paste and stirred thoroughly. Butanediol can better dissolve the crown ether.

[0138] In this embodiment, the cooling temperature is 8-10℃, and the stirring speed is 100-150 rpm.

[0139] The crown ether needs to be added after emulsification to avoid damage to the crown ether by stirring during the emulsification process. The stirring speed at the cooling temperature is 100-150 rpm.

[0140] In this embodiment, the self-repairing eye care cream cross-linked by the mussel adhesive protein and hyaluronic acid described above is applied to a massage stick, which includes a massage head embedded with an electromagnet connected to an electric current. When in use, the massage head is connected to an electric current, and the magnetic field generated by the electromagnet stimulates the self-repairing eye care cream applied to the eye and promotes the cross-linking of hyaluronic acid and mussel adhesive protein through the conductive effect of graphene.

[0141] Specifically, the massage head is embedded with an electromagnet that can generate a magnetic field. The electromagnet is connected to a power source through an electric wire, and generates a magnetic field when an electric current passes through it. The self-repairing eye care cream is evenly applied to the massage head of the massage stick.

[0142] Before use, ensure that the electromagnet of the massage stick is connected to the power source and the electric current is turned on. When the electromagnet is powered on, the magnetic field generated will act on the self-repairing eye care cream applied to the eye. The addition of graphene in the self-repairing eye care cream can enhance the effect of the magnetic field and promote the cross-linking of hyaluronic acid and mussel adhesive protein, thereby enhancing the self-repairing ability of the eye care cream. Gently place the massage head on the eye and use the magnetic field and the conductive effect of graphene, as well as the shape and design of the massage head, to gently massage the eye. This massage can promote blood circulation in the eye and relieve eye fatigue, while the moisturizing and lubricating effects of the self-repairing eye care cream can improve the health of the eye skin. The amount of graphene added can also be different according to age, for example, 0.01 for 18-28 years old, 0.05 for 29-35 years old, 0.08 for 35-45 years old, and 0.1 for 45 years old and above.

[0143] The specific structure of the massage stick described above is that a small electromagnet is embedded in the massage head, which can generate a magnetic field.

[0144] The electromagnet is connected to the power source through an internal electric wire, ensuring that the electric current can pass smoothly. A rechargeable battery or an adapter is used to provide power, ensuring portability and ease of use. A current switch and a knob are included to control the on-off and intensity of the electric current.

[0145] The self-repairing eye care cream is evenly applied to the massage head of the massage stick. Ensure that the electromagnet of the massage stick is connected to the power source and the electric current is turned on. When the electromagnet is powered on, the magnetic field generated will act on the self-repairing eye care cream applied to the eye. Gently place the massage head on the eye and use the magnetic field and the conductive effect of graphene, as well as the shape and design of the massage head, to gently massage the eye.

[0146] In this embodiment, the surface of the massage head that contacts the eye is designed as a honeycomb groove surface, and the depth of each groove is 2-5 mm.

[0147] The honeycomb groove surface can store a certain amount of eye cream, so that the eye cream can be treated by the electromagnetic field in the groove.

[0148] In this embodiment, the current is 50-80 microamperes.

[0149] Experimental results

[0150] To further illustrate the experimental results of the present application, the following comparison is made.

[0151] Experimental Example 1

[0152] The mussel mucin and hyaluronic acid cross-linked self-repairing eye care cream is prepared from the following components by weight,

[0153] Stearic acid 1-5 parts, cetostearyl alcohol 1-3 parts, distilled monostearate glyceride 1-3 parts, vaseline 5-10 parts, butanediol 1-5 parts, menthol 0.1-1 part, borneol 0.1-1 part, lactic acid bacteria fermentation extract 1-5 parts, jasmine extract 1-3 parts, wolfberry extract 1-3 parts, panax notoginseng extract 1-3 parts, cassia seed extract 1-3 parts, giant knotweed extract 1-3 parts, chrysanthemum extract 1-3 parts, leontopodium leontopodioides extract 1-3 parts, nipagin ethyl 0.01-0.1 parts, nipagin methyl 0.01-0.1 parts, oat extract 1-5 parts, decapeptide-4 0.01-0.1 parts, mussel mucin 0.1-1 parts, polysaccharide protein 0.5-2 parts, modified graphene 0.01-0.1 parts, and the balance of pure water.

[0154] The modified graphene is prepared by the following method:

[0155] Step one,

[0156] The formula amount of hyaluronic acid is dissolved in PBS buffer, and the mass and volume ratio of the hyaluronic acid and PBS is 3mg:9mL.

[0157] N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide are added to the hyaluronic acid solution, and the molar ratio of N-hydroxysuccinimide to 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide is 1:1.

[0158] Stir for 1-2h at room temperature to activate the carboxyl groups on the hyaluronic acid. After the reaction time is over, add ethanolamine to the first mixed solution, and the amount of ethanolamine added accounts for 02% of the first mixed solution, to neutralize the unreacted N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, and obtain activated hyaluronic acid;

[0159] Step two,

[0160] The graphene of the formula amount is dispersed in PBS buffer solution, and the mass to volume ratio of the graphene to the PBS buffer solution is: 2 mg: 10 ml to obtain a graphene dispersion liquid. The activated hyaluronic acid solution and the graphene dispersion liquid are mixed, and the weight ratio of the activated hyaluronic acid solution to the graphene dispersion liquid is 1:1.

[0161] The pH of the graphene dispersion was adjusted to neutral using PBS buffer, and the reaction was monitored by UV-visible spectroscopy until the hyaluronic acid and graphene were covalently bonded to obtain modified graphene.

[0162] The preparation method of the self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid is characterized by comprising the following steps:

[0163] Oil phase preparation:

[0164] Mix stearic acid, cetostearyl alcohol, distilled glyceryl monostearate and vaseline in a container, and heat the mixture to 60°C to keep the oil phase in liquid form;

[0165] Aqueous phase preparation:

[0166] Add butylene glycol, ethylparaben, methylparaben, oat extract, decapeptide-4, mussel mucin and polysaccharide protein to purified water, heat to 50°C, detect and adjust the pH of the aqueous phase to 6-7, and maintain the aqueous phase state;

[0167] Extract processing:

[0168] Dilute menthol, borneol, lactic acid bacteria fermentation extract, jasmine extract, wolfberry extract, Panax notoginseng extract, cassia seed extract, knotweed extract, chrysanthemum extract and leontopodium edulis extract in pure water respectively, so that each extract is completely dissolved;

[0169] Emulsification process:

[0170] Before starting emulsification, the oil phase and the water phase were heated to 60°C and kept at a constant temperature. The heated oil phase was slowly added to the water phase for a pre-mixing stage. The stirring speed in the pre-mixing stage was 200 rpm, and then the stirring speed was 9000 rpm to ensure thorough and uniform mixing. During the emulsification process, the pH was regularly monitored and adjusted to a pH of 6-7 to obtain an emulsion.

[0171] Add modified graphene:

[0172] Disperse the modified graphene in purified water to form a uniform suspension, slowly add the suspension to the emulsion, and continue stirring until a paste is obtained;

[0173] During the emulsification process, a formulated amount of tetraalkylammonium salt is also added.

[0174] The weight ratio of the crown ether to the emulsion is 1:9. After emulsification is completed, the crown ether is dissolved in butanediol and then slowly added to the cooled emulsion or paste and fully stirred. The cooling temperature is 9°C and the stirring speed is 100.

[0175] Applied to:

[0176] Objective: To evaluate the efficacy of the eye ointment in this study.

[0177] Volunteers: Recruit 20 healthy adult volunteers.

[0178] The volunteers underwent blood tests and medical history surveys and were divided into three groups: Group 1 (18 to 25 years old), Group 2 (28 to 35 years old), and Group 3 (40 to 50 years old).

[0179] Drug application method: apply the self-repairing eye care ointment to the massage stick. When using, the massage head is connected to the current and the massage head applies the eye ointment. The frequency of use is once in the morning and once in the evening. The dosage of each application is 2 mg. The massage time is 5 minutes and it is used for 4 weeks.

[0180] Comparative Example 1

[0181] The difference between Comparative Example 1 and Experimental Example 1 is that the modified graphene is omitted and 1 to 2 parts of hyaluronic acid are added.

[0182] Comparative Example 2

[0183] The difference between Comparative Example 2 and Experimental Example 1 is that 1-5 parts of lactic acid bacteria fermentation extract, 1-3 parts of jasmine extract, 1-3 parts of wolfberry extract, 1-3 parts of Panax notoginseng extract, 1-3 parts of cassia seed extract, 1-3 parts of knotweed extract, 1-3 parts of chrysanthemum extract, 1-3 parts of edelweiss extract, 0.01-0.1 parts of ethylparaben, 0.01-0.1 parts of methylparaben, 1-5 parts of oat extract, 0.01-0.1 parts of decapeptide-4, and 0.1-1 parts of mussel mucin are omitted, and the other components and operations are the same as those in Example 1.

[0184] The data comparison in Table 1 is obtained

[0185] Table 1

[0186]

[0187]

[0188] Table 2

[0189] Material Type Resistivity (Ω·M) Conductivity improvement percentage Experimental Example 1 10^12 Comparative Example 1 10^14

[0190] As can be seen from Table 2, the electrical conductivity is improved by 90%.

[0191] It can be seen from the above data that the eye ointment prepared by the present invention can effectively relieve eye symptoms. When using the ingredients of the formula of the present invention, the efficacy of the formula of the present invention can be better improved by adding graphene.

[0192] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid, characterized in that: The composition comprises the following components in parts by weight: 1-5 parts stearic acid, 1-3 parts cetostearyl alcohol, 1-3 parts distilled monostearate glyceryl, 5-10 parts petrolatum 1-5 parts of butylene glycol, 0.1-1 part of menthol, 0.1-1 part of borneol, 1-5 parts of lactic acid bacteria fermented extract, 1-3 parts of jasmine extract, 1-3 parts of wolfberry extract, 1-3 parts of Panax notoginseng extract, 1-3 parts of cassia seed extract, 1-3 parts of knotweed extract, 1-3 parts of chrysanthemum extract, 1-3 parts of leontopodium edelweiss extract, 0.01-0.1 part of ethylparaben, 0.01-0.1 part of methylparaben, 1-5 parts of oat extract, 0.01-0.1 part of decapeptide-4, 0.1-1 part of mussel mucin, 0.5-2 parts of polysaccharide protein, 0.01-0.1 part of modified graphene, and the balance is pure water; The modified graphene is prepared by the following method: Step 1: Activate hyaluronic acid: Dissolve the formulated amount of hyaluronic acid in PBS buffer, with the mass to volume ratio of hyaluronic acid to PBS buffer being 1-2 mg:8-10 mL; Adding formulated amounts of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide to a hyaluronic acid solution, wherein the molar ratio of N-hydroxysuccinimide to 1-ethyl-3-(3-dimethylaminopropyl)carboxydiimide is 1-2:1-2; Stirring at room temperature for 1 to 2 hours to activate the carboxyl groups on the hyaluronic acid to obtain a first mixed solution. After the reaction time is completed, adding ethanolamine to the first mixed solution in an amount of 0.2% to 0.5% of the first mixed solution to obtain activated hyaluronic acid; Step 2: Dispersing a formulated amount of graphene in another PBS buffer solution, wherein the mass-to-volume ratio of the graphene to the PBS buffer solution is 2 mg to 3 mg: 10 ml to 20 ml, to obtain a graphene dispersion solution; adjusting the pH of the graphene dispersion solution to neutrality using the PBS buffer solution; mixing the activated hyaluronic acid solution with the graphene dispersion solution, wherein the weight ratio of the activated hyaluronic acid solution to the graphene dispersion solution is 1 to 2:10 to 1; The reaction is monitored by UV-visible spectroscopy until the hyaluronic acid is covalently bonded to the graphene to obtain modified graphene.

2. The self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid according to claim 1, characterized in that: The modified graphene was purified by: The modified graphene was placed in a dialysis bag and dialyzed with PBS buffer for 12 to 24 hours. The mixture was then centrifuged at 10,000 to 15,000 rpm for 8 to 15 minutes, and the supernatant was collected.

3. The preparation method of the self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid according to claim 1 or 2, characterized in that: The following steps are involved: Oil phase preparation: Mix stearic acid, cetostearyl alcohol, distilled glyceryl monostearate and vaseline in a container, and heat the mixture to 60-70°C to keep the oil phase in liquid form; Aqueous phase preparation: Add butylene glycol, ethylparaben, methylparaben, oat extract, decapeptide-4, mussel mucin and polysaccharide protein to purified water, heat to 40°C to 50°C, detect and adjust the pH of the aqueous phase to 6-7, and maintain the aqueous phase state; Extract processing: Dilute menthol, borneol, lactic acid bacteria fermentation extract, jasmine extract, wolfberry extract, Panax notoginseng extract, cassia seed extract, knotweed extract, chrysanthemum extract and leontopodium edulis extract in pure water respectively, so that each extract is completely dissolved; Emulsification process: Before starting emulsification, the oil phase and the water phase are heated to 40°C to 60°C respectively, and the temperature is maintained constant. The heated oil phase is slowly added to the water phase to perform a pre-mixing stage. The stirring speed of the pre-mixing stage is 100 rpm to 300 rpm, and then the stirring speed is 1000 rpm to 10000 rpm to ensure sufficient and uniform mixing. During the emulsification process, the pH is regularly monitored and adjusted to 6 to 7 to obtain an emulsion; Add modified graphene: Disperse the modified graphene in purified water to form a uniform suspension, slowly add the suspension to the emulsion, and continue stirring until a paste is obtained; After emulsification is completed, the formulated amount of crown ether is also added.

4. The method for preparing the self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid according to claim 3, wherein: The weight ratio of the crown ether to the emulsion is 1:8-10.

5. The preparation method of the self-repairing eye health cream cross-linked with mussel mucin and hyaluronic acid according to claim 4, characterized in that, The crown ether is any one of tetraalkylammonium salt, crown sulfide or tetraphenylphosphine.

6. The method for preparing the self-repairing eye care ointment cross-linked with mussel mucin and hyaluronic acid according to claim 5, characterized in that: Dissolve the crown ether in butanediol and then slowly add it to the cooled emulsion or paste with thorough stirring.

7. The method for preparing the self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid according to claim 6, characterized in that: The cooling temperature is 8-10°C and the stirring speed is 100-150 rpm.

8. A 4D molding method for a self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid, characterized in that: A self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid prepared according to any one of claims 3 to 7 is used, and the self-repairing eye care cream is applied to a massage stick, the massage stick comprising a massage head, the massage head being composed of an electromagnet, the electromagnet being connected to an electric current, the self-repairing eye care cream being applied to the eye through the massage head, and when in use, the massage head is connected to an electric current, the magnetic field generated by the electromagnet stimulates the self-repairing eye care cream applied to the eye and causes cross-linking of the hyaluronic acid and the mussel mucin through the conductive effect of graphene.

9. The 4D molding method of the self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid according to claim 8, characterized in that, The surface of the massage head that contacts the eyes is formed into a honeycomb groove surface, and the depth of each groove in the groove surface is 2mm to 5mm.

10. The 4D molding method of the self-repairing eye care cream cross-linked with mussel mucin and hyaluronic acid according to claim 9, characterized in that: The current is 50 to 80 microamperes.

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