Method for preparing nanogold compound for treating peritoneal cancer based on quercetin
By preparing quercetin-modified gold nanocomplexes, the problems of large side effects and limited therapeutic effects in the treatment of peritoneal cancer were solved, targeted treatment of peritoneal cancer cells and improved drug delivery efficiency were achieved, toxicity to normal tissues was reduced and the therapeutic effect was enhanced.
Patent Information
- Application Number
- CN202410398655.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-03
- Publication Date
- 2025-10-14
AI Technical Summary
Existing methods for treating peritoneal cancer have significant side effects, difficulty in early diagnosis, and limited therapeutic effects. In particular, peritoneal cancer is often difficult to detect early, making treatment more difficult.
By preparing a quercetin-modified gold nanoparticle complex, utilizing the anti-cancer properties of quercetin and the biocompatibility of gold nanoparticles, the nanoparticle size is controlled to 15nm-25nm, and modified with a surfactant to ensure that quercetin is successfully modified onto the gold nanoparticle surface, achieving targeted treatment of peritoneal cancer cells.
It achieves precise positioning and treatment of peritoneal cancer cells, reduces toxicity to normal tissues, enhances drug bioavailability, prolongs circulation time in the body, improves drug delivery efficiency, and alleviates multidrug resistance.
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of nanomaterials, and in particular to a method for treating peritoneal cancer by preparing a nano-gold complex based on quercetin. Background Art
[0002] Peritoneal cancer is a rare but malignant tumor with a steadily increasing incidence, yet current treatment options remain challenging. Conventional treatments such as surgical resection, radiotherapy, and chemotherapy have limitations in improving patient survival. This is particularly true because peritoneal cancer is often difficult to diagnose early and often progresses to advanced stages, further complicating treatment.
[0003] Traditional treatments are often associated with severe side effects and damage to normal tissues. Therefore, finding more effective and precise treatments is crucial for improving the survival of peritoneal cancer patients. Recent advances in nanotechnology have brought new opportunities to cancer treatment. Nanomaterials possess unique biological properties that can enhance therapeutic efficacy and reduce side effects by regulating drug release and improving drug targeting.
[0004] Quercetin, a natural compound derived from traditional Chinese medicine, has gradually attracted attention in the field of anti-cancer research. Quercetin possesses anti-inflammatory, antioxidant, and anti-tumor properties, but its practical application is limited by poor solubility and low bioavailability. Therefore, combining quercetin with nanotechnology, through the modification of gold nanoparticles, may bring new breakthroughs in the treatment of peritoneal cancer.
[0005] This invention aims to overcome the limitations of existing treatments by providing a quercetin-modified gold nanoparticle drug complex to improve the treatment of peritoneal cancer. By combining the anticancer properties of quercetin with the biocompatibility of gold nanoparticles, it is hoped that this complex will achieve highly effective targeted therapy for peritoneal cancer cells, alleviate patient suffering, and improve survival rates. Summary of the Invention
[0006] The purpose of the present invention is to solve the above technical problems and provide a method for treating peritoneal cancer by preparing a nano-gold complex based on quercetin.
[0007] The above technical objectives of the present invention are achieved through the following technical solutions:
[0008] S1: Ensure all glassware and tools used are rigorously cleaned to avoid the introduction of impurities. Dissolve chloroaurate (HAuCl4) in high-purity water to prepare a gold ion solution. Typically, the concentration used is in the range of 1mM to 10mM.
[0009] S2: Add reducing agent, usually sodium citrate and sodium borohydride (NaBH4), to reduce gold ions to gold nanoparticles. The specific steps are to slowly add sodium borohydride (NaBH4) solution to the gold ion solution at room temperature 20-25°C, adjust the drop rate to maintain a moderate reaction rate, and stir the solution to ensure uniform mixing.
[0010] S3: By adjusting the molar ratio of chloroaurate HAuCl4 and sodium borohydride NaBH4, the size of gold nanoparticles is controlled between 15nm-25nm.
[0011] S4: During or after the synthesis of gold nanoparticles, introduce the surfactant polyvinylpyrrolidone (PVP) for modification, continue to stir the mixed solution to ensure that the surfactant fully wraps the gold nanoparticles. The reaction time may range from 5 hours to 24 hours, depending on the desired particle characteristics.
[0012] S5: Use a centrifuge to separate the gold nanoparticles from the reaction mixture, and use an appropriate solvent to wash the particles to remove unreacted reagents and impurities.
[0013] S6: Use transmission electron microscopy (TEM), scanning electron microscopy (SEM), etc. to ensure that the shape, size and surface properties of the gold nanoparticles meet the requirements of 15nm-25nm.
[0014] S7: Quercetin is modified to thiol quercetin by hydroxyl group, the reaction condition is at room temperature, the reaction time is 24 hours, and the structure of functionalized quercetin is confirmed by nuclear magnetic resonance (NMR).
[0015] S8: React the functionalized quercetin with the surface modified gold nanoparticles, observe the morphology of the quercetin modified gold nanoparticles by transmission electron microscopy (TEM), and ensure that the gold nanoparticles are uniformly dispersed and successfully modified.
[0016] S9: Remove unreacted substances by centrifugal filtration, then measure the particle size distribution of the gold nanoparticles using dynamic light scattering (DLS), and characterize the quercetin modified gold nanoparticles using Fourier transform infrared spectroscopy (FTIR) to ensure that the quercetin is successfully modified to the surface of the gold nanoparticles.
[0017] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present application are:
[0018] 1. Since the quercetin modified gold nanoparticles have surface functionalization, they can bind to specific receptors or biomarkers on the surface of peritoneal cancer cells, thus exhibiting excellent targeting properties. This helps to achieve precise positioning and treatment of peritoneal cancer cells in vivo.
[0019] 2. Gold nanoparticles have good biocompatibility in the body, reducing toxicity and side effects on normal tissues. Quercetin-modified gold nanoparticles can more safely circulate through the bloodstream, helping to mitigate adverse reactions caused by treatment.
[0020] 3. Quercetin itself has anti-cancer properties, and its combination with gold nanoparticles may produce a synergistic effect. Quercetin-modified gold nanoparticles can affect peritoneal cancer cells through multiple pathways, including inhibiting cell proliferation, inducing cell apoptosis, and inhibiting angiogenesis, thereby achieving a more effective anti-cancer effect.
[0021] 4. Due to the small particle size and surface functionalization of quercetin-modified gold nanoparticles, it is expected to improve the delivery efficiency of drugs by increasing the bioavailability of nanomedicines, prolonging the circulation time of drugs in the body, and enhancing the enrichment of drugs in tumor tissues.
[0022] 5. The multiple effects of quercetin-modified gold nanoparticles may help alleviate the multidrug resistance of peritoneal cancer cells to drugs. This may be achieved by changing intracellular signaling, increasing drug accumulation in cells, or through other mechanisms. DETAILED DESCRIPTION
[0023] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.
[0024] Example 1:
[0025] A method for treating peritoneal cancer by preparing a nanogold complex based on quercetin, the method comprising the following steps:
[0026] S1: Ensure all glassware and tools used are rigorously cleaned to avoid the introduction of impurities. Dissolve chloroaurate (HAuCl4) in high-purity water to prepare a gold ion solution. Typically, the concentration used is in the range of 1mM to 10mM.
[0027] S2: Add a reducing agent, usually sodium citrate and sodium borohydride (NaBH4), to reduce gold ions to gold nanoparticles. The specific steps are to slowly add sodium borohydride (NaBH4) solution to the gold ion solution at room temperature 20℃-25℃, adjust the addition speed to maintain a moderate reaction rate, and stir the solution to ensure uniform mixing.
[0028] S3: By adjusting the molar ratio of HAuCl4 and sodium borohydride NaBH4, the size of gold nanoparticles can be controlled between 15nm-25nm.
[0029] S4: During or after the gold nanoparticle synthesis, introduce the surfactant polyvinylpyrrolidone (PVP) for modification. Continuously stir the mixed solution to ensure that the surfactant is fully coated with the gold nanoparticles. The reaction time may range from 5 to 24 hours, depending on the desired particle characteristics.
[0030] S5: Use a centrifuge to separate the gold nanoparticles from the reaction mixture, and wash the particles with an appropriate solvent to remove unreacted reagents and impurities.
[0031] S6: Use transmission electron microscopy (TEM), scanning electron microscopy (SEM) and other techniques to ensure that the shape, size and surface properties of the gold nanoparticles meet the 15nm-25nm requirements.
[0032] S7: Quercetin was modified to mercaptoquercetin via hydroxyl groups at room temperature for 24 hours. The structure of the functionalized quercetin was confirmed by nuclear magnetic resonance (NMR).
[0033] S8: The functionalized quercetin was reacted with the surface-modified gold nanoparticles, and the morphology of the quercetin-modified gold nanoparticles was observed by transmission electron microscopy (TEM) to ensure that the gold nanoparticles were uniformly dispersed and successfully modified.
[0034] S9: Unreacted substances were removed by centrifugal filtration, and the particle size distribution of gold nanoparticles was determined by dynamic light scattering (DLS). The quercetin-modified gold nanoparticles were characterized by Fourier transform infrared spectroscopy (FTIR) to ensure that quercetin was successfully modified onto the surface of the gold nanoparticles.
[0035] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A method for treating peritoneal cancer by preparing a nanogold complex based on quercetin, characterized in that: The method for treating peritoneal cancer by preparing a nano-gold complex using quercetin comprises the following steps: S1: Ensure all glassware and tools used are rigorously cleaned to avoid the introduction of impurities. Dissolve chloroaurate (HAuCl4) in high-purity water to prepare a gold ion solution. Typically, the concentration used is in the range of 1mM to 10mM. S2: Add a reducing agent, usually sodium citrate and sodium borohydride (NaBH4), to reduce gold ions to gold nanoparticles. The specific steps are to slowly add sodium borohydride (NaBH4) solution to the gold ion solution at room temperature 20℃-25℃, adjust the addition speed to maintain a moderate reaction rate, and stir the solution to ensure uniform mixing. S3: By adjusting the molar ratio of HAuCl4 and sodium borohydride NaBH4, the size of gold nanoparticles can be controlled between 15nm-25nm. S4: During or after the gold nanoparticle synthesis, introduce the surfactant polyvinylpyrrolidone (PVP) for modification. Continuously stir the mixed solution to ensure that the surfactant is fully coated with the gold nanoparticles. The reaction time may range from 5 to 24 hours, depending on the desired particle characteristics. S5: Use a centrifuge to separate the gold nanoparticles from the reaction mixture, and wash the particles with an appropriate solvent to remove unreacted reagents and impurities. S6: Use transmission electron microscopy (TEM), scanning electron microscopy (SEM) and other techniques to ensure that the shape, size and surface properties of the gold nanoparticles meet the 15nm-25nm requirements. S7: Quercetin was modified to mercaptoquercetin via hydroxyl groups at room temperature for 24 hours. The structure of the functionalized quercetin was confirmed by nuclear magnetic resonance (NMR). S8: The functionalized quercetin was reacted with the surface-modified gold nanoparticles, and the morphology of the quercetin-modified gold nanoparticles was observed by transmission electron microscopy (TEM) to ensure that the gold nanoparticles were uniformly dispersed and successfully modified. S9: Unreacted substances were removed by centrifugal filtration, and the particle size distribution of gold nanoparticles was determined by dynamic light scattering (DLS). The quercetin-modified gold nanoparticles were characterized by Fourier transform infrared spectroscopy (FTIR) to ensure that quercetin was successfully modified onto the surface of the gold nanoparticles.
2. The method for treating peritoneal cancer by preparing a nano-gold complex based on quercetin according to claim 1, characterized in that: The gold nanoparticle reducing agent is used at a temperature of 22°C.
3. The method for treating peritoneal cancer by preparing a gold nanocomposite based on quercetin according to claim 1, characterized in that: The size of the gold nanoparticles is 20 nm.
4. The method for treating peritoneal cancer by preparing a nano-gold complex based on quercetin according to claim 1, characterized in that: The surfactant polyvinylpyrrolidone (PVP) was introduced for modification, the mixed solution was continuously stirred, and the reaction time was controlled within 12 hours.