A protein blotting apparatus

Abstract

This invention belongs to the field of protein antibody incubation technology and provides a protein blotting processing device, comprising: a base; a limiting post fixedly disposed on the base, one end of the limiting post having a cam component, the cam component having at least one protrusion and / or one concave portion; a turntable rotatably disposed relative to the limiting post, comprising a first fixed plate and a second fixed plate, the cam component being located between the first fixed plate and the second fixed plate, and the cam component and the second fixed plate having a preset distance; at least one first push rod movably disposed on the lower side of the second fixed plate, one end of which can abut against the cam component, the cam component being able to drive the first push rod to reciprocate along its own axis; a reaction tank movably disposed on the upper side of the second fixed plate, and passing through the second fixed plate and movably connected to the other end of the first push rod. By adopting the above structure, the reaction tank can be oscillated simultaneously with the rotation of the turntable, aided by the cam component and the first push rod.

Description

Technical Field

[0001] This invention belongs to the field of protein antibody incubation, and in particular refers to a protein blotting processing device. Background Technology

[0002] Western blotting is an experimental technique used to detect and analyze specific proteins, widely applied in biomedical research. Its main steps are as follows: Proteins in the sample are separated using polyacrylamide gel electrophoresis (PAGE) based on their molecular weight. The separated proteins are then transferred to a solid support (such as a PVDF or nitrocellulose membrane) for immobilization. Non-specific binding sites on the membrane are then blocked with a protein containing no target protein (such as bovine serum albumin BSA or skim milk powder) to reduce background noise. First, the membrane is incubated with a specific primary antibody to bind the target protein. Then, a secondary antibody (usually labeled with an enzyme or fluorescent dye) is used to detect the target protein bound to the primary antibody. The bound secondary antibody is detected by chemiluminescence, fluorescence, or colorimetry, thereby identifying the target protein and analyzing its expression level.

[0003] Most existing protein blotting devices are semi-automatic. Furthermore, the various parts of the existing devices are independent of each other, with many driving devices, complex structures and large sizes. In addition, manual intervention is still required for some processes, making it impossible to achieve truly fully automated operation. Summary of the Invention

[0004] This invention provides a protein blotting processing device that solves the problems of existing devices in the background art, which are mostly semi-automatic processing structures and cannot achieve truly fully automated processing. At the same time, existing devices have separate modules, multiple driving devices, and large overall device size.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A protein blotting processing apparatus, comprising:

[0007] Base;

[0008] A limiting post, one end of which is fixedly mounted on the base, the limiting post having a preset length, and a fixedly mounted cam component at the end of the limiting post away from the base, the cam component having at least one recess and / or one protrusion;

[0009] A turntable is rotatable relative to the limiting post. The turntable includes a first fixed plate and a second fixed plate. The first fixed plate is disposed near the base, and the second fixed plate is disposed near the cam component. The cam component is located between the first fixed plate and the second fixed plate, and a preset distance is provided between the cam component and the second fixed plate.

[0010] At least one first push rod is movably disposed on the lower side of the second fixed plate. One end of the first push rod can abut against the cam component, and the first push rod can reciprocate along its own axis.

[0011] The reaction tank is movably disposed on the upper side of the second fixed plate. The reaction tank is provided with a first connecting part, and the second fixed plate is provided with a through first slot. The first connecting part passes through the first slot and is movably connected to the other end of the first push rod. The first push rod can drive the reaction tank to swing back and forth relative to the second fixed plate.

[0012] In some embodiments, the contact end between the first push rod and the cam component is provided with a first roller, the first roller being rotatably mounted on the first push rod, and the first roller being able to abut against the cam component.

[0013] In some embodiments, both the recess and the convex portion are arc-shaped structures, and the convex portion and the recess are connected by a smooth transition.

[0014] In some embodiments, the lower side of the second fixed plate is provided with a first sliding groove, the first push rod is movably inserted through the first sliding groove, and a plurality of second rollers are provided on both sides of the first sliding groove, and the plurality of second rollers abut against the side of the first push rod.

[0015] In some embodiments, a first return spring is also included, one end of which is connected to the outside of the first slide groove, and the other end of which is connected to the first push rod.

[0016] In some embodiments, the reaction tank is provided with a suitable incubation box, which is detachably mounted on the reaction tank.

[0017] In some embodiments, a third fixing plate is also included, which is disposed between the first fixing plate and the second fixing plate, and is connected to the first fixing plate and the second fixing plate respectively;

[0018] The second and third fixed disks are provided with a plurality of test tube assemblies. Each test tube assembly includes a first support on the second fixed disk and a heat preservation cavity on the third fixed disk. The first support is provided with a plurality of first mounting holes, which communicate with the heat preservation cavity. The heat preservation cavity is provided with a first adapter and a first cooling chip. The first adapter is used to support the bottom of the test tube, and the first cooling chip is used to cool the heat preservation cavity.

[0019] In some embodiments, a first radiator and a temperature monitoring sensor are also provided on the outside of the insulation cavity. The first radiator includes a plurality of first heat dissipation fins arranged at intervals between each other, and the temperature monitoring sensor is used to detect the temperature inside the insulation cavity.

[0020] In some embodiments, the first bracket is further provided with a cleaning tank, the cleaning tank including a first cleaning hole and a second cleaning hole, the inner diameter of the first cleaning hole being larger than the inner diameter of the second cleaning hole, and the depth of the first cleaning hole being greater than the depth of the second cleaning hole.

[0021] In some embodiments, the central axes of the plurality of first mounting holes, first cleaning holes and second cleaning holes are all located on the same circumference.

[0022] In some embodiments, the first support and the reaction tank are alternately disposed on the second fixed plate.

[0023] In some embodiments, a second cooling chip and a cooling base are also included, wherein the second cooling chip is fixedly disposed relative to the third fixing disk, and the second cooling chip and the cooling base are connected;

[0024] The reaction tank is provided with a second slot, and a cooling contact seat is provided at the second slot. The cooling contact seat contacts the incubation box, and one end of the cooling seat abuts against the cooling contact seat.

[0025] In some embodiments, a smooth guide structure is provided between the cooling contact seat and the cooling seat. The smooth guide structure includes a first arc-shaped groove disposed on the cooling contact seat and a first arc-shaped boss disposed at one end of the cooling seat. The first arc-shaped groove and the first arc-shaped boss are adapted to each other. The rotation axis of the cooling contact seat relative to the second fixed plate coincides with the center of the first arc-shaped groove.

[0026] In some embodiments, the third fixed plate is further provided with a first heat insulation seat, the first heat insulation seat is provided with a through first cooling channel, the cooling seat passes through the first cooling channel and abuts against the cooling contact seat, wherein the first heat insulation seat is provided with a first clearance groove, the position of the first clearance groove corresponds to the position of the first connecting part, and the first push rod can extend into the first clearance groove and connect with the first connecting part.

[0027] In some embodiments, the first heat insulation seat is provided with a second heat sink, the second heat sink including a plurality of parallel second heat dissipation fins, the second heat dissipation fins being located below the third fixing plate.

[0028] In some embodiments, a liftable aspiration needle is also included, wherein the aspiration needle is provided with a first aspiration channel, and one end of the aspiration needle is provided with a first fixing block, wherein the first fixing block is provided with a first sub-channel, a second sub-channel and a third sub-channel, and the first sub-channel, the second sub-channel and the third sub-channel are all connected to the first aspiration channel.

[0029] In some embodiments, the system further includes a waste liquid pump, a pure water pump, and a closed liquid pump mounted on the base, wherein the waste liquid pump is connected to the first sub-channel via a first valve, the pure water pump is connected to the second sub-channel via a second valve, and the closed liquid pump is connected to the third sub-channel via a third valve.

[0030] In some embodiments, a plunger pump is provided between the second valve and the second sub-channel, and the plunger pump is connected in series with the second valve.

[0031] In some embodiments, the system further includes a first connecting tube, a first lifting block, a first supporting block, and a second lifting block. The second lifting block is connected to the first supporting block, and a preset distance is provided between the second lifting block and the first lifting block. One end of the first connecting tube is connected to the first fixing block, and the other end of the first connecting tube is connected to the first lifting block. The first lifting block is disposed on the first supporting block, and the first lifting block can be detached from the first supporting block. The first lifting block, the first supporting block, and the second lifting block move in the same direction, and the central axis of the first connecting tube is parallel to the central axis of the aspiration needle.

[0032] In some embodiments, a first connecting rod and a first guide rod are arranged parallel to each other. The first guide rod is provided with a first guide sleeve, which is connected to the first lifting block. The first lifting block is movably inserted through the first guide rod, and the first connecting rod passes through the first lifting block and is connected to the first lifting block.

[0033] Compared with the prior art, the beneficial effects of this application are:

[0034] This invention features a limiting post on a base, with one end fixed relative to the base and a cam component at the other end. The cam component has at least one recess and / or one protrusion. A turntable is rotatably mounted on the limiting post, allowing it to rotate around the limiting post. At least one first push rod is mounted on a second fixed plate of the turntable. The first push rod can reciprocate linearly relative to the second fixed plate along its axial length. One end of the first push rod abuts against the cam component, and the other end is movably connected to a first connecting part of a reaction tank that is oscillating on the second fixed plate. The rotation of the turntable drives the first push rod to rotate synchronously, changing the abutment position of the first push rod on the cam component and causing it to reciprocate linearly. Because the first push rod is connected to the reaction tank, it simultaneously drives the reaction tank to oscillate relative to the second fixed plate during the rotation of the turntable. Only one driving device is needed to change the position of the reaction tank relative to the limiting post and simultaneously oscillate the reaction tank, achieving uniform mixing of the substances within the reaction tank.

[0035] Additional aspects and advantages of this application will be set forth in part in the description which follows, and will become apparent from the description or may be learned by practice of this application. Attached Figure Description

[0036] Figure 1 This is a first perspective view of a protein blotting processing apparatus according to the present invention;

[0037] Figure 2 An exploded view of the turntable of a protein blotting processing apparatus according to the present invention;

[0038] Figure 3 for Figure 2 Another view;

[0039] Figure 4 This is a top view of the reaction tank transmission structure of a protein blotting processing apparatus according to the present invention.

[0040] Figure 5 This is a perspective view of the reaction tank transmission structure of a protein blotting processing apparatus according to the present invention.

[0041] Figure 6 This is a perspective view of a test tube assembly of a protein blotting processing device according to the present invention;

[0042] Figure 7 for Figure 6 Exploded view in the image;

[0043] Figure 8 for Figure 7 Cross-sectional view of the intermediate cleaning tank;

[0044] Figure 9 An exploded view of the cooling module of the reaction tank in a protein blotting apparatus of the present invention.

[0045] Figure 10 This is a cross-sectional view of the cooling module of the reaction tank in a protein blotting apparatus according to the present invention.

[0046] Figure 11 This is a simplified schematic diagram of the reaction tank transmission structure of a protein blotting processing apparatus according to the present invention.

[0047] Figure 12 This is an exploded view of the internal structure of the reaction tank of a protein blotting processing apparatus according to the present invention;

[0048] Figure 13 This is a schematic diagram of the drive structure of the turntable in a protein blotting processing device according to the present invention.

[0049] Figure 14 for Figure 13 Mid-section view;

[0050] Figure 15 This is a cross-sectional view of the aspiration needle and its connection structure of a protein blotting processing device according to the present invention.

[0051] Figure 16 This is a second perspective view of a protein blotting processing apparatus according to the present invention;

[0052] Figure 17 for Figure 16 Enlarged view at point B in the middle;

[0053] Figure 18 An exploded view of the lifting structure of the sampling needle in a protein blotting processing device of the present invention.

[0054] Figure 19 An exploded view of the first groove of a protein blotting processing apparatus according to the present invention;

[0055] Figure 20 This is a schematic diagram of the pump body of a protein blotting processing device according to the present invention.

[0056] Figure 21 This is a schematic diagram of the pipe connections of various components of a protein blotting processing device according to the present invention. Detailed Implementation

[0057] The present application will be further described in detail below with reference to the accompanying drawings. In the description of the embodiments, unless otherwise stated, the terms "left", "right", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and are not intended to indicate or imply that the present application must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the present application.

[0058] like Figure 1 , Figure 2 , Figure 5 as well as Figure 13 As shown, a protein imprinting processing device provided by the present invention includes a base 1000, a limiting post 200 is provided on the base 1000, one end of the limiting post 200 is fixed relative to the base 1000, and the limiting post 200 is provided with a preset length. A fixed cam component 201 is provided at the end of the limiting post 200 away from the base. The cam component 201 has a certain thickness and at least one protrusion 20111 and / or one concave portion 20112 are provided on the cam component 201; for example, only one protrusion 20111 or only one concave portion 20112 is provided on the cam component 201, or the protrusion 20111 and the concave portion 20112 are alternately provided.

[0059] A turntable is rotatably mounted on a limiting post 200. The turntable includes a first fixed plate 101 and a second fixed plate 102. The first fixed plate 101 is disposed near the base 1000, and the second fixed plate 102 is disposed near the cam component 201. The cam component 201 is located between the first fixed plate 101 and the second fixed plate 102. A preset distance is provided between the second fixed plate 102 and the cam component 201 to ensure that the second fixed plate 102 can rotate relative to the cam component 201.

[0060] At least one first push rod 1022 is movably disposed on the lower side of the second fixed plate 102, such as... Figure 4 and Figure 5 As shown, one end of the first push rod 1022 can abut against the cam component 201. During the rotation of the turntable, one end of the first push rod 1022 is always in contact with the cam component 201. For example, one end of the first push rod 1022 abuts against the concave part 20112 or the convex part 20111. As the rotation of the turntable causes the position of the first push rod 1022 relative to the limiting post 200 to change, under the action of the cam component 201, the first push rod 1022 can reciprocate linearly along its own axial length.

[0061] The reaction tank 1028 is movably mounted on the upper side of the second fixed plate 102. The number of reaction tanks 1028 is the same as the number of first push rods 1022. A first connecting part 10281 is provided on the reaction tank 1028, such as... Figure 12As shown, a first slot 1021 is provided on the second fixed disk 102. A first connecting part 10281 passes through the first slot 1021 and is movably connected to the other end of the first push rod 1022. Through the reciprocating linear movement of the first push rod 1022, the reaction tank 1028 can be driven to reciprocate relative to the second fixed disk 102, thereby allowing the antibody in the reaction tank 1028 to be fully mixed and combined with the blocking membrane for antibody incubation. In this embodiment, the reaction tank 1028 is rotatably mounted on the second fixed disk 102 through a hole-shaft connection.

[0062] This application achieves this by setting a limiting post 200 on the base 1000, and a cam component 201 on the limiting post 200, combined with a first push rod 1022 set on the second fixed disk 102. By rotating the turntable relative to the limiting post 200, one end of the first push rod 1022 can switch between the concave part 20112 and the convex part 20111 of the cam component 201, driving the first push rod 1022 to move back and forth linearly. This allows the reaction tank 1028, which is movably connected to the other end of the first push rod 1022, to swing back and forth relative to the second fixed disk 102, so that the antibody and blocking membrane in the reaction tank 1028 can be fully shaken and mixed for incubation. This achieves the simultaneous rotation of the turntable and the reciprocating swing of the reaction tank 1028 by a single driving component.

[0063] In a preferred embodiment, the movable connection between the first push rod 1022 and the first connecting portion 10281 of the reaction tank 1028 is as follows: Figure 11 and 12 As shown, a through hole is provided at one end of the first push rod 1022, and a corresponding through U-shaped groove 102810 is provided on the first connecting part 10281. The two are connected by a pin. By providing the U-shaped groove 102810, the movement interference in the vertical direction of the plane where the second fixed plate 102 is located can be avoided when the reaction tank 1028 swings.

[0064] Alternatively, the U-shaped groove 102810 can be replaced with a through hole of a larger diameter, thereby compensating for the movement gap of the first connecting part 10281 in the direction perpendicular to the plane of the second fixed plate 102.

[0065] Alternatively, the U-shaped groove 102810 can be set on the first push rod 1022, wherein the length direction of the U-shaped groove 102810 should preferably be in the direction perpendicular to the second fixed plate 102. The U-shaped groove 102810 can also be inclined in the direction perpendicular to the plane of the second fixed plate 102, as long as the reaction tank 1028 can swing relative to the second fixed plate 102810.

[0066] In this embodiment, as Figure 11As shown, the cam component 201 includes four recesses 20112 and four protrusions 20111, corresponding to four first push rods 1022 and four reaction grooves 1028. The number of reaction grooves 1028 and first push rods 1022 can be set according to experimental requirements. For example, the number of recesses 20112 and protrusions 20111 can both be three, or both can be five, or other numbers. The number is not limited by this invention, as long as it allows for installation on the second fixed plate 102.

[0067] In one embodiment, such as Figure 4 and Figure 5 As shown, in a preferred embodiment, a first roller 1023 is provided at the contact end between the first push rod 1022 and the cam component 201. The first roller 1023 can rotate relative to the first push rod 1022 and abuts against the cam component 201. During the rotation of the turntable, the first roller 1023 is configured as a rolling structure, reducing the frictional resistance between the first push rod 1022 and the cam component 201, thereby making the rotation of the turntable smoother. In this embodiment, the first roller 1023 has a preset width, and the cam component 201 has a suitable thickness, thereby ensuring the contact surface between the first roller 1023 and the cam component 201, and ensuring the stability of the transmission between the first push rod 1022 and the cam component 201.

[0068] Furthermore, both the recess 20112 and the convex portion 20111 of the cam component 201 have arc-shaped structures, and a smooth transition connection is used between the convex portion 20111 and the recess 20112. Specifically, as shown... Figure 4 , Figure 5 as well as Figure 13 As shown, the smooth transition connection means that the connection between the convex part 20111 and the concave part 20112 of the cam component 201 is directly tangent or connected by a smooth plane, and both the convex part 20111 and the concave part 20112 are tangent to the smooth plane. By adopting the above structure, the contact between the first roller 1023 and the cam component 201 is a tangent structure, which can reduce the contact surface between the first roller 1023 and the cam component 201 on the first push rod 1022. At the same time, combined with the rotating first roller 1023, the frictional resistance between the first roller 1023 and the cam component 201 during the rotation of the turntable is further reduced.

[0069] In one embodiment, such as Figure 3 , Figure 4 as well as Figure 5 As shown, to facilitate the reciprocating linear movement of the first push rod 1022 relative to its own axial length, a first sliding groove is provided on the lower side of the second fixed plate 102, and the first push rod 1022 is movably inserted into the first sliding groove. Specifically, as shown... Figure 19As shown, the first slide groove consists of a slide groove base 1026 and a slide groove cover plate 1025. The slide groove base 1026 is disposed on the lower side of the second fixed plate 102. The slide groove cover plate 1025 is detachably disposed on the slide groove base 1026. A first slot is provided on the slide groove base 1026. The slide groove cover plate 1025 covers the first slot to form the first slide groove. The slide groove cover plate 1025 provides support for the first push rod 1022 in the vertical direction of the plane where the second fixed plate 102 is located.

[0070] Alternatively, the first groove structure can also be a bushing structure. For example, the first push rod 1022 is a cylindrical structure, and the bushing is sleeved on the lower side of the second fixed disk 102. The first push rod 1022 can move axially relative to the bushing, which can also achieve the above purpose.

[0071] Furthermore, a plurality of second rollers 1024 are provided on both sides of the first slide groove, and each of the second rollers 1024 abuts against the side of the first push rod 1022. Specifically, in this embodiment, as shown... Figure 4 and Figure 5 As shown, the second rollers 1024 include four, arranged in pairs at both ends of the slide base 1026. All four second rollers 1024 are rotatably mounted relative to the second fixed plate 102. The four second rollers 1024 pass through the slide base 1026 and are fixedly connected to the second fixed plate 102. During the installation of the second rollers 1024, the slide base 1026 is simultaneously further fixed, simplifying the assembly process of the slide base 1026. In this embodiment, when the second rollers 1024 are installed, the side of the first push rod 1022, which contacts the second rollers 1024, does not contact the first slide. The first slide only supports the first push rod 1022, thereby reducing the contact area between the first push rod 1022 and the first slide during movement. This also converts some sliding friction into rolling friction, reducing the frictional resistance during the movement of the first push rod 1022 and making its reciprocating linear movement smoother.

[0072] In one embodiment, such as Figure 4 , Figure 5 as well as Figure 19As shown, to facilitate the movement of the first push rod 1022 from the protrusion 20111 of the cam component 201 to the concave portion 20112 of the cam component 201, a first return spring (not shown) is also included. The first return spring enables the first push rod 1022 to move from the protrusion 20111 to the concave portion 20112. Specifically, a first connecting post 10252 is provided on the first push rod 1022, and a second connecting post 10251 is provided on the slide cover plate 1025. One end of the first return spring is connected to the first connecting post 10252, and the other end of the first return spring is connected to the second connecting post 10251. The first return spring is located on the outside of the first slide groove. By adopting the above structure, the extension and retraction direction of the first return spring is parallel to the movement direction of the first push rod 1022, so that the elastic force generated by the first return spring is completely used for the power of the first push rod 1022 to move and reset.

[0073] Optionally, the first connecting post 10252 can be disposed at one end of the first push rod 1022 near the reaction tank 1028. When the first push rod 1022 moves from the concave part 20112 to the convex part 20111 of the cam component 201, the first return spring is in a stretched state. When the turntable continues to rotate, the first push rod 1022 moves from the convex part 20111 to the concave part 20112 of the cam component 201 under the action of the first return spring.

[0074] Optionally, the first connecting post 10252 can also be disposed between the first slide groove and the cam component 201. In this case, when the first push rod 1022 moves toward the reaction tank 1028, the first reset spring is compressed. When the turntable continues to rotate, under the action of the first reset spring, the first push rod 1022 can move from the protrusion 20111 of the cam component 201 to the concave part 20112.

[0075] In one embodiment, such as Figure 12 As shown, to facilitate antibody binding to the blocking membrane and antibody incubation, a detachable incubation box 10284 is provided within the reaction tank 1028. The shape of the incubation box 10284 is adapted to the internal structure of the reaction tank 1028, thereby ensuring that the position of the incubation box 10284 within the reaction tank 1028 is relatively fixed. In this embodiment, the incubation box 10284 can only incubate one type of antibody at a time. Optionally, a partition can be provided within the incubation box 10284 to divide it into multiple independent parts, such as two, three, or four independent parts, to accommodate different antibody incubation requirements according to experimental needs. Optionally, the incubation box 10284 can also be for single use.

[0076] In one embodiment, such as Figure 2 and Figure 3As shown, the turntable also includes a third fixed plate 103, which is rotatably set relative to the limiting post 200. The third fixed plate 103 is set between the first fixed plate 101 and the second fixed plate 102. The third fixed plate 103 is fixedly connected to the first fixed plate 101 through multiple first fixed posts 1012. The third fixed plate 103 is set parallel to the first fixed plate 101 and the second fixed plate 102, and the third fixed plate 103 is fixedly connected to the second fixed plate 102 through multiple second fixed posts 1033.

[0077] Several test tube assemblies are arranged on the second fixed plate 102 and the third fixed plate 103, such as Figure 1 , Figure 6 as well as Figure 7 As shown, the test tube assembly includes multiple test tubes of different sizes, each used to hold different antibodies or washing solutions. Specifically, the test tube assembly includes a first support 10271 mounted on a second mounting plate 102 and an insulation shell 10311 mounted on a third mounting plate 103. The insulation shell 10311 contains an insulation cavity 10316. The first support 10271 has multiple first mounting holes, and the second mounting plate has first through holes 1027 corresponding to the positions, sizes, and numbers of the first mounting holes. Figure 4 As shown, multiple first mounting holes communicate with the insulation cavity 10316. A first adapter 10317 is disposed within the insulation cavity 10316. The first adapter 10317 has multiple first support positions adapted to test tubes. The first adapter 10317 is mainly used for mounting and placing multiple test tubes, supporting the bottom of the multiple test tubes. In addition, a first cooling chip 10318 is disposed within the insulation cavity 10316. The first cooling chip 10318 is used for cooling within the insulation cavity 10316 to reach the temperature required by the antibody reagents in the test tubes. For ease of assembly, the side of the insulation shell 10311 has an opening communicating with the insulation cavity 10316. An insulation foam block 10319 is disposed at the opening to seal the opening. The first cooling chip is disposed on the insulation foam block 10319 and located within the insulation cavity 10316, thereby facilitating the assembly of the first cooling chip 10318.

[0078] Furthermore, a first radiator 10314 and a temperature detection sensor 10312 are also provided on the outer side of the insulation shell 10311. The first radiator 10314 is located on the lower side of the insulation shell 10311, such as... Figure 3 and Figure 6As shown, a plurality of first mounting slots 1032 are provided on the third fixed plate 103. The heat insulation shell 10311 is fixed at the first mounting slots 1032. The first radiator 10314 includes a plurality of first heat dissipation fins arranged at intervals, which are connected by a plurality of first heat dissipation pipes 10315. A first heat dissipation block 10313 is provided at one end of the first heat dissipation pipe 10315. The first radiator 10314 is fixed to the outer wall of the heat insulation cavity 10316 by the first heat dissipation block 10313. A temperature detection sensor 10312 is provided inside the heat insulation cavity 10316 to monitor the real-time temperature inside the heat insulation cavity 10316, thereby regulating the temperature inside the heat insulation cavity in real time through the first cooling chip 10318.

[0079] In one embodiment, to simplify the operation of the device, the first support 10271 and the reaction tank 1028 are alternately arranged, both located near the outer edge of the second fixed disk 102. In this embodiment, there are four first supports 10271 and four reaction tanks 1028, arranged alternately. Each first support 10271 corresponds to one reaction tank 1028. That is, multiple test tubes on the first support 10271 contain the same consumables required for the experiment. For example, in this embodiment, two large-capacity test tubes and two small-capacity test tubes are used. Typically, since the antibody reagent requirement is small, the two small-capacity test tubes are generally used to hold the antibody reagent, and the two large-capacity test tubes are generally used to hold the corresponding washing solution. For example, when using antibody reagent A, the washing solution corresponding to antibody reagent A is placed; when using antibody reagent B, the washing solution corresponding to antibody reagent B is used. When a large amount of reagents is required, two large-capacity test tubes can be used to hold antibody reagents, and two small-capacity test tubes can be used to hold the corresponding washing solution. The specific application can be set according to the user's actual needs.

[0080] Furthermore, in this embodiment, for ease of differentiation, the same color is used to mark the first support 10271 and reaction tank 1028 in the same group. In this embodiment, the first support 10271 and reaction tank 1028 are divided into 4 groups, using 4 different colors, such as red, white, black, and blue, so that users can know the corresponding experimental situation in each group of first support 10271 and reaction tank 1028 at any time.

[0081] In one embodiment, such as Figure 9 and Figure 10 As shown, in order to provide a relatively stable incubation temperature for the reaction tank 1028, a second cooling element 10324 and a cooling base 10323 are also included. The second cooling element 10324 is fixedly mounted relative to the third fixed plate 103, and the second cooling element 10324 and the cooling base 10323 are connected; wherein, as Figure 12As shown, a second slot 10282 is provided on the reaction tank 1028, and a cooling contact seat 10283 is provided at the second slot 10282. The cooling contact seat 10283 is located inside the reaction tank 1028 and has a cooling transfer surface of a certain area. The cooling transfer surface is in full contact with the incubation box 10284 to ensure cooling transfer efficiency. The cooling transfer surface is in contact with the incubation box 10284 located inside the reaction tank 1028. One end of the cooling seat 10323 abuts against the cooling contact seat 10283 to realize heat transfer.

[0082] Furthermore, since the reaction tank 1028 is rotatably mounted relative to the second fixed disk 102, a smooth guide structure is provided between the cooling contact seat 10283 and the cooling seat 10323 to ensure both the cooling transfer effect and to prevent motion interference during the rotation of the reaction tank 1028. Specifically, the smooth guide structure includes a first arc-shaped groove 102831 disposed on the cooling contact seat 10283 and a first arc-shaped boss 103231 disposed at one end of the cooling seat 10323. The first arc-shaped boss 103231 and the first arc-shaped groove 102831 are adapted to each other, thereby increasing the contact area between the cooling seat 10323 and the cooling contact seat 10283 and ensuring the efficiency of cooling transfer. It should be noted that the axis of rotation of the reaction tank 1028 relative to the second fixed disk 102 coincides with the center of the first arc groove 102831, thus ensuring that the cooling seat 10323 does not push up the cooling contact seat 10283 during the rotation of the reaction tank 1028, and the relative position of the contact point between the cooling contact seat 10283 and the cooling seat 10323 remains unchanged, preventing the reagent mixture in the incubation box 10284 on the reaction tank 1028 from spilling out.

[0083] Alternatively, the first arc-shaped groove 102831 can be set on the cooling base 10323, and the first arc-shaped boss 103231 can be set on the cooling contact base 10283, which can also achieve the above effect.

[0084] In one embodiment, to achieve heat insulation and prevent the heat inside the device from affecting the cooling effect during the second cooling element 10324 process, a first heat insulation seat 10321 is also provided. Specifically, as shown... Figure 3 and Figure 9As shown, the third fixed plate 103 has a second mounting slot 1031, and the first heat insulation seat 10321 is fixedly installed in the second mounting slot 1031 of the third fixed plate 103. A through first cooling channel 10322 is provided on the first heat insulation seat 10321, and the cooling seat 10323 passes through the first cooling channel 10322 and abuts against the cooling contact seat 10283 to transfer cooling. It should be noted that because the first push rod 1022 is connected to the first connecting part 10281 of the reaction tank 1028, the cooling seat 10323 is located directly below the reaction tank 1028. Therefore… A first clearance groove 103210 is provided on the first heat insulation seat 10321. The position of the first clearance groove 103210 corresponds to the position of the first connecting part 10281. The first connecting part 10281 can be located in the first clearance groove 103210. When the first connecting part 10281 rotates with the reaction tank 1028, the first connecting part 10281 does not contact the first clearance groove 103210. A part of the first push rod 1022 is connected to the first connecting part 10281 through the first clearance groove 103210. While achieving heat insulation, it can also avoid interference with the movement of the reaction tank 1028.

[0085] Furthermore, to ensure heat dissipation, a second radiator 10325 is provided below the first heat insulation seat 10321. The second radiator 10325 has a similar structure to the first radiator 10314, and includes multiple parallel and spaced second heat dissipation fins, wherein the second heat dissipation fins are located below the third fixing plate 103. In this embodiment, both the second and first heat dissipation fins are located between the first fixing plate 101 and the third fixing plate 103, as shown below. Figure 1 As shown, multiple heat dissipation holes are provided on the side wall of the first housing 1001 of the overall device. The first heat dissipation fins and the second heat dissipation fins follow the rotation of the turntable and dissipate heat to the outside through the heat dissipation holes.

[0086] In one embodiment, such as Figure 15 As shown, the protein blotting processing device also includes a liftable aspiration needle 401, on which a first aspiration channel is provided. At one end of the aspiration needle 401, a first fixing block 402 is provided. The first fixing block 402 is provided with three independent first sub-channels 4021, second sub-channels 4022 and third sub-channels 4023. The first sub-channels 4021, second sub-channels 4022 and third sub-channels 4023 are all connected to the first aspiration channel.

[0087] In one embodiment, a cleaning groove 10310 is provided on the first bracket 10271, and the cleaning groove 10310 includes a first cleaning hole 103101 and a second cleaning hole 103102, such as Figure 8As shown, the inner diameter of the first cleaning hole 103101 is larger than the inner diameter of the second cleaning hole 103102, and the depth of the first cleaning hole 103101 is greater than the depth of the second cleaning hole 103102.

[0088] During the cleaning process of the aspiration needle, the first cleaning hole 103101 is used to clean the first aspiration channel of the aspiration needle 401. When cleaning the outer wall of the aspiration needle 401, the aspiration needle 401 moves to the second cleaning hole 103102 for cleaning. With the help of the smaller inner diameter of the second cleaning hole 103102, the water pressure impacts the inner wall of the second cleaning hole 103102, which is then reflected onto the outer wall of the aspiration needle 401 to clean the outer wall.

[0089] In one embodiment, to simplify the movement control of the aspiration needle 401, only the aspiration needle 401 is allowed to reciprocate up and down. The central axes of the multiple first mounting holes, the first cleaning holes 103101, and the second cleaning holes 103102 are all located on the same circumference 1020. Figure 4 As shown, the projection of the aspiration needle 401 on the plane of the second fixed plate 102 is located on the circumference line 1020. Therefore, the aspiration needle 401 can be accurately delivered simply by rotating the turntable and moving the aspiration needle 401 up and down.

[0090] Furthermore, in one embodiment, such as Figure 16 , Figure 17 as well as Figure 20 As shown, it also includes a waste liquid pump 501, a pure water pump 502, and a closed-loop liquid pump 503 mounted on the base. The waste liquid pump 501 is connected to the first sub-channel 4021 via a first valve 5011; the pure water pump 502 is connected to the second sub-channel 4022 via a second valve 5021; and the closed-loop liquid pump 503 is connected to the third sub-channel 4023 via a third valve 5031. One end of the waste liquid pump 501 is also connected to a waste liquid tank 601 via a pipe. The waste liquid pump 501 is used for the incubation box 10. Waste liquid generated during cleaning within compartment 284 is extracted and recycled into waste liquid container 601. One end of pure water pump 502 is connected to an external pure water container 602. Pure water pump 502 cleans the aspiration needle 401 and incubation box 10284 to prepare for the next experiment, avoiding cross-contamination. Blocking solution pump 503 is also connected to an external blocking solution container 603. Before antibody incubation, blocking solution is drawn by blocking solution pump 503 and delivered to a clean incubation box 10284. It should be noted that the connections between the above modules are all via flexible hoses, which will not be elaborated upon here. The first valve 5011, second valve 5021, and third valve 5031 can be solenoid valves for automated control. Solenoid valves are existing technology and will not be described in detail here.

[0091] Furthermore, to simplify the connection structure and enable the absorption and addition of antibody reagents, such as... Figure 21 As shown, a plunger pump 409 is installed between the second valve 5021 and the second sub-channel 4022. The inlet and outlet of the plunger pump 409 are connected to the second valve 5021 and the second sub-channel 4022 via flexible hoses, respectively. By placing the plunger pump 409 on the pure water pipeline, cross-contamination can be effectively avoided, ensuring the accuracy of experimental data. Simultaneously, since the amount of antibody reagent drawn is small, there is no risk of antibody being drawn into the pure water tank 602. When drawing or adding antibody reagent, the plunger pump 409 performs suction or exhaust actions to complete the above operations.

[0092] In one embodiment, such as Figure 17 As shown, to prevent the aspiration needle 401 from colliding with the needle, the lifting assembly of the liftable aspiration needle 401 includes a first connecting tube 403, a first lifting block 404, a first supporting block 405, and a second lifting block 4072. The second lifting block 404 is fixedly connected to the first supporting block 405. A preset distance is provided between the second lifting block 4072 and the first lifting block 404. One end of the first connecting tube 403 is connected to the first fixed block 402, and the other end of the first connecting tube 403 is connected to the first lifting block 404. The first lifting block 404 is disposed on the first supporting block 405. The first lifting block 404 and the first supporting block 405 are separable. The first lifting block 404, the second lifting block 4072, and the first supporting block 405 move in the same direction. The central axis of the first connecting tube 403 is parallel to the central axis of the aspiration needle 401. The three hoses connected to the first sub-channel 4021, the second sub-channel 4022, and the third sub-channel 4023 are all connected to the first valve 5011, the second valve 5021, and the third valve 5031 through the first channel 4031 in the first connecting pipe 403 and the first wiring channel 4041 provided on the first lifting block 404. Of course, it is known that the hoses between the first lifting block 404 and the first valve 5011, the second valve 5021, and the third valve 5031 are of sufficient length to allow the first lifting block 404 to reciprocate and move up and down.

[0093] Furthermore, such as Figure 18As shown, to ensure stable lifting and lowering movement of the first lifting block 404 and the second lifting block 4072, a first guide rod 408 and a first connecting rod 410 arranged parallel to each other are also included. A first guide sleeve 4081 is provided on the first guide rod 408, and the first guide sleeve 4081 can move relative to the first guide rod 408. The first guide sleeve 4081 is connected to the first lifting block 404. In this embodiment, there are two first guide rods 408 and two first guide sleeves 4081. A through hole adapted to the first connecting rod 410 is provided on the first lifting block 404. The first connecting rod 410 is movably inserted through the through hole. One end of the first connecting rod 410 is connected to the second lifting rod 4072, and the other end of the first connecting rod passes through the through hole on the first lifting block 404 and is connected to the first lifting block 405. There are two first connecting rods 410, which are arranged parallel to the first guide rods 408. When the second lifting block 4072 moves downward, the first lifting block 405 moves downward simultaneously. Due to its own gravity, the first lifting block 404 moves downward along the length of the first guide rod 408. When the suction needle 401 touches the bottom, the second lifting block 4072 can still drive the first lifting block 405 to move downward. Since the suction needle 401 touches the bottom, the first lifting block 404 and the first lifting block 405 separate and remain stationary, thereby preventing the suction needle 401 from colliding with the needle and protecting the suction needle 401 and the entire device.

[0094] In this embodiment, the reciprocating movement of the second lifting block 4072 is mainly embodied by a screw and nut structure. Specifically, it includes a first support 400 mounted on the base 1000, a first motor 407 mounted on the first support 400, a first screw 4071 mounted at the output end of the first motor 407, the first screw 4071 being rotatably mounted on the first support 400 via a bearing, the first screw 4071 being parallel to the first guide rod 408, and a first nut mounted on the second lifting block 4072. The second lifting block 4072 is movably mounted on the first guide rod 408 for limiting, thereby, in conjunction with the first nut and the first lead screw 4071, the second lifting block 4072 reciprocates by rotating the first lead screw 4071 in both directions. It should be noted that the other end of the first lead screw 4071 is suspended in the air, and corresponding clearance holes are provided on the first lifting block 404 and the first lifting block 405, so as to ensure that the other end of the first lead screw 4071 can avoid contact with the first lifting block 404 and the first lifting block 405.

[0095] Optionally, the lifting structure of the second lifting block 4072 can also be a synchronous belt and synchronous pulley structure, which can also realize the reciprocating lifting movement of the second lifting block 4072.

[0096] In one embodiment, since the turntable needs to rotate, this application employs a driving device to drive it, enabling the reaction tank 1028 located on the second fixed disk 102 to rotate simultaneously while the turntable rotates. Specifically, as shown... Figure 13 and 14 As shown, the turntable's driving device includes a second motor 301 mounted on the base 1000, a first driving synchronous pulley mounted at the output end of the second motor 301, and a first driven synchronous pulley 303 rotatably mounted on the limiting post 200 via bearings. A first fixed disk 101 is fixedly mounted on the first driven synchronous pulley 303, and the first fixed disk 101 and the first driven synchronous pulley 303 rotate synchronously. A first synchronous belt 302 is wound between the first driving synchronous pulley and the first driven synchronous pulley 303, thereby achieving power transmission. The turntable rotates in both directions by the forward and reverse rotation of the second motor 301.

[0097] Optionally, the transmission structure of the first active synchronous pulley and the first driven synchronous pulley 303 can be replaced by a gear assembly. It is understood that the power drive structure and composition of the turntable are not limited by the present invention, as long as the rotation of the turntable is achieved.

[0098] In one embodiment, since refrigeration requires electrical energy, and both the first refrigeration element 10318 and the second refrigeration element 10324 move by resisting the limiting post, the system also includes a conductive slip ring 1014 disposed on the limiting post 200 and a conductive carbon brush 1013 disposed on the first fixed disk 101. The conductive carbon brush 1013 is fixedly disposed on the first fixed disk 101 by a carbon brush holder 1011. The conductive carbon brush 1013 and the conductive slip ring 1014 achieve sliding electrical contact, which can realize stable transmission of current and signal. This is prior art and will not be described in detail here. The limiting post 200 has a first wire channel 2010 inside, and a through wire hole 20101 on its side. The external wire is electrically connected to the conductive slip ring 1014 through the first wire channel 2010 and the wire hole 20101. The conductive carbon brush 1013 is then electrically connected to the first cooling chip 10318 and the second cooling chip 10324 through the wire. Since the conductive carbon brush 1011 remains in a fixed position relative to the first fixed plate 101 and rotates synchronously with the turntable, the problem of wire tangling can be effectively avoided during the rotation of the turntable.

[0099] The above description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention. These improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A protein blotting processing device, characterized in that, include: Base; A limiting post, one end of which is fixedly mounted on the base, the limiting post having a preset length, and a fixedly mounted cam component at the end of the limiting post away from the base, the cam component having at least one recess and / or one protrusion; A turntable, rotatable relative to the limiting post, includes a first fixed plate and a second fixed plate connected to each other. The first fixed plate is positioned near the base, and the second fixed plate is positioned near the cam component. The cam component is located between the first and second fixed plates, and a preset distance is provided between the cam component and the second fixed plate. At least one first push rod is movably disposed on the lower side of the second fixed plate, one end of the first push rod abuts against the cam component, and the first push rod can reciprocate along its own axis. The reaction tank is movably disposed on the upper side of the second fixed plate. The reaction tank is provided with a first connecting part, and the second fixed plate is provided with a through first slot. The first connecting part passes through the first slot and is movably connected to the other end of the first push rod. The first push rod can drive the reaction tank to swing back and forth relative to the second fixed plate.

2. The protein blotting processing apparatus according to claim 1, characterized in that, The first push rod has a first roller at the contact end with the cam component. The first roller is rotatably mounted on the first push rod and abuts against the cam component.

3. The protein blotting processing apparatus according to claim 1, characterized in that, Both the concave and convex portions are arc-shaped structures, and the convex and concave portions are connected by a smooth transition.

4. The protein blotting processing apparatus according to claim 1, characterized in that, The lower side of the second fixed plate is provided with a first sliding groove, the first push rod is movably inserted through the first sliding groove, and a plurality of second rollers are provided on both sides of the first sliding groove, and the plurality of second rollers abut against the side of the first push rod.

5. The protein blotting processing apparatus according to claim 4, characterized in that, It also includes a first return spring, one end of which is connected to the outside of the first slide groove, and the other end of which is connected to the first push rod.

6. The protein blotting processing apparatus according to claim 1, characterized in that, The reaction tank is equipped with a suitable incubation box, which is detachably mounted on the reaction tank.

7. The protein blotting processing apparatus according to claim 6, characterized in that, It also includes a third fixing plate that is rotatably configured relative to the limiting post. The third fixing plate is disposed between the first fixing plate and the second fixing plate, and is connected to the first fixing plate and the second fixing plate respectively. The second and third fixed disks are provided with a plurality of test tube assemblies. Each test tube assembly includes a first support on the second fixed disk and a heat preservation cavity on the third fixed disk. The first support is provided with a plurality of first mounting holes, which communicate with the heat preservation cavity. The heat preservation cavity is provided with a first adapter and a first cooling chip. The first adapter is used to support the bottom of the test tube, and the first cooling chip is used to cool the heat preservation cavity.

8. The protein blotting processing apparatus according to claim 7, characterized in that, The outer side of the insulation cavity is also provided with a first radiator and a temperature monitoring sensor. The first radiator includes a plurality of first heat dissipation fins arranged at intervals. The temperature monitoring sensor is used to detect the temperature inside the insulation cavity.

9. The protein blotting processing apparatus according to claim 7, characterized in that, The first bracket is also provided with a cleaning tank, which includes a first cleaning hole and a second cleaning hole. The inner diameter of the first cleaning hole is larger than the inner diameter of the second cleaning hole, and the depth of the first cleaning hole is larger than the depth of the second cleaning hole.

10. The protein blotting processing apparatus according to claim 9, characterized in that, The central axes of the multiple first mounting holes, the first cleaning holes, and the second cleaning holes are all located on the same circumference.

11. The protein blotting processing apparatus according to claim 10, characterized in that, The first support and the reaction tank are alternately arranged on the second fixed plate.

12. The protein blotting processing apparatus according to claim 11, characterized in that, It also includes a second cooling chip and a cooling base, wherein the second cooling chip is fixedly disposed relative to the third fixed plate, and the second cooling chip and the cooling base are connected; The reaction tank is provided with a second slot, and a cooling contact seat is provided at the second slot. The cooling contact seat contacts the incubation box, and one end of the cooling seat abuts against the cooling contact seat.

13. The protein blotting processing apparatus according to claim 12, characterized in that, A smooth guide structure is provided between the cooling contact seat and the cooling base. The smooth guide structure includes a first arc-shaped groove on the cooling contact seat and a first arc-shaped boss at one end of the cooling base. The first arc-shaped groove and the first arc-shaped boss are adapted to each other. The rotation axis of the cooling contact seat relative to the second fixed plate coincides with the center of the first arc-shaped groove.

14. The protein blotting processing apparatus according to claim 13, characterized in that, The third fixed plate is also provided with a first heat insulation seat, and the first heat insulation seat is provided with a through first refrigeration channel. The refrigeration seat passes through the first refrigeration channel and abuts against the refrigeration contact seat. The first heat insulation seat is provided with a first clearance groove, the position of the first clearance groove corresponds to the position of the first connecting part, and the first push rod can extend into the first clearance groove and connect with the first connecting part.

15. The protein blotting processing apparatus according to claim 14, characterized in that, The first heat insulation seat is provided with a second heat sink, which includes a plurality of parallel second heat sink fins, and the second heat sink fins are located on the lower side of the third fixed plate.

16. A protein blotting apparatus according to any one of claims 1-15, characterized in that, It also includes a liftable aspiration needle, which has a first aspiration channel and a first fixing block at one end. The first fixing block has a first sub-channel, a second sub-channel and a third sub-channel, which are all connected to the first aspiration channel.

17. The protein blotting processing apparatus according to claim 16, characterized in that, It also includes a waste liquid pump, a pure water pump, and a closed liquid pump mounted on the base, wherein the waste liquid pump is connected to the first sub-channel through a first valve, the pure water pump is connected to the second sub-channel through a second valve, and the closed liquid pump is connected to the third sub-channel through a third valve.

18. The protein blotting processing apparatus according to claim 17, characterized in that, A plunger pump is provided between the second valve and the second sub-channel, and the plunger pump is connected in series with the second valve.

19. The protein blotting processing apparatus according to claim 16, characterized in that, It also includes a first connecting tube, a first lifting block, a first supporting block, and a second lifting block. The second lifting block is connected to the first supporting block, and there is a preset distance between the second lifting block and the first lifting block. One end of the first connecting tube is connected to the first fixing block, and the other end of the first connecting tube is connected to the first lifting block. The first lifting block is disposed on the first supporting block, and the first lifting block can be detached from the first supporting block. The first lifting block, the first supporting block, and the second lifting block move in the same direction, and the central axis of the first connecting tube is parallel to the central axis of the aspiration needle.

20. The protein blotting processing apparatus according to claim 19, characterized in that, It also includes a first connecting rod and a first guide rod arranged parallel to each other. The first guide rod is provided with a first guide sleeve, which is connected to the first lifting block. The first lifting block is movably inserted through the first guide rod, and the first connecting rod passes through the first lifting block and is connected to the first lifting block.

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