Liquid injection module, immunoassay equipment and liquid injection method

Abstract

The invention relates to a liquid injection module, an immunoassay device and a liquid injection method.The immunoassay device comprises an incubation module and the liquid injection module, the incubation module comprises a reaction disc cover, the liquid injection module comprises a support, a liquid injection assembly, a heating assembly and a driving assembly, the driving assembly can drive the support to slide on the reaction disc cover, and the liquid injection assembly is installed on the support; the liquid injection assembly comprises a liquid injection head and a liquid bottle assembly, a to-be-injected object is stored in the liquid bottle assembly, the heating assembly comprises a heating pipeline and a heating part, the heating part can heat the heating pipeline, one end of the heating pipeline can be communicated with the liquid bottle assembly, and the other end of the heating pipeline is communicated with the liquid injection head. After the heating assembly heats the to-be-injected object, the to-be-injected object is injected into the reaction cup through the liquid injection assembly, the to-be-injected object does not need to be heated in the reaction cup, the incubation time is shortened, and the incubation effect is guaranteed. The liquid injection method is achieved through the liquid injection module, liquid injection, cleaning and to-be-injected object replacement can be achieved, incubation in the immunoassay process is facilitated, and the immunoassay efficiency is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of liquid injection devices, in particular to a liquid injection module, an immunoassay device and a liquid injection method. BACKGROUND

[0002] In the process of immunoassay, the to-be-injected substance needs to be injected into the reaction cup and incubated. However, the to-be-injected substance is generally stored at low temperature, and it takes a long time to heat the to-be-injected substance to a specified temperature after being injected into the reaction cup, and the incubation time is long, and the incubation effect is not good enough.

[0003] Therefore, there is an urgent need for a liquid injection module, an immunoassay device and a liquid injection method to solve the above technical problems. SUMMARY

[0004] The first object of the present application is to provide a liquid injection module to at least solve one of the above problems.

[0005] To achieve the above object, the present application provides a liquid injection module, comprising:

[0006] a bracket configured to be slidably arranged on a reaction disc cover;

[0007] a liquid injection assembly mounted on the bracket, the liquid injection assembly comprising a liquid injection head and a liquid bottle assembly, the liquid bottle assembly storing a to-be-injected substance;

[0008] a heating assembly comprising a heating pipeline and a heating element, the heating element being capable of heating the heating pipeline, one end of the heating pipeline being capable of communicating with the liquid bottle assembly, and the other end communicating with the liquid injection head;

[0009] a driving assembly capable of driving the bracket to slide.

[0010] Further, the liquid injection module further comprises a push block mounted on the bracket, the push block being provided with a insertion hole, and the driving assembly is capable of being connected with the insertion hole.

[0011] Further, the driving assembly comprises a pipette, the pipette being movably arranged relative to the reaction disc cover, and the pipette is capable of being inserted into the insertion hole.

[0012] Further, the heating assembly further comprises a fixing seat, the fixing seat being provided with a heating pipeline groove in a serpentine distribution, the heating pipeline being mounted in the heating pipeline groove, and the heating element being attached to the heating pipeline.

[0013] Further, the heating assembly further comprises a heating cover plate, and the heating element is mounted between the heating cover plate and the fixing seat.

[0014] Further, the liquid injection module further comprises a reset assembly, the reset assembly comprises a tension spring, one end of the tension spring is fixed relative to the reaction disc cover, and the other end is fixed relative to the support.

[0015] Further, the liquid injection module further comprises a cleaning assembly, the cleaning assembly comprises a cleaning liquid bottle storing cleaning liquid, the liquid injection assembly further comprises a liquid discharge pipeline, the cleaning liquid bottle and the liquid bottle assembly are selectively communicated on the liquid discharge pipeline, and one end of the liquid discharge pipeline is communicated with the heating pipeline.

[0016] Further, the liquid injection module further comprises a drag chain assembly and a slide rail, the drag chain assembly comprises a drag chain, the slide rail is fixedly installed on the reaction disc cover, one end of the drag chain is fixedly installed on the reaction disc cover, and the other end is fixedly installed on the support, and the support is slidably arranged on the slide rail.

[0017] A second object of the present application is to provide an immunoassay device to at least solve one of the above problems.

[0018] To achieve the above object, the present application provides an immunoassay device, comprising:

[0019] An incubation module comprising a reaction disc cover;

[0020] The liquid injection module according to any one of the above solutions.

[0021] A third object of the present application is to provide a liquid injection method to at least solve one of the above problems.

[0022] To achieve the above object, the present application provides a liquid injection method, the liquid injection module further comprises a cleaning assembly, the cleaning assembly comprises a cleaning liquid bottle storing cleaning liquid, the liquid injection assembly further comprises a liquid discharge pipeline, one end of the liquid discharge pipeline can be communicated with the liquid bottle assembly through the liquid discharge pipeline, the cleaning liquid bottle and the liquid bottle assembly are selectively communicated on the liquid discharge pipeline, and the liquid injection method comprises the following steps:

[0023] Step S1: preheating the to-be-injected material: the liquid bottle assembly and the liquid discharge pipeline are communicated, the to-be-injected material enters the liquid discharge pipeline from the liquid bottle assembly, the heating assembly is started, and the to-be-injected material enters the heating pipeline from the liquid bottle assembly through the liquid discharge pipeline and is preheated to a specified temperature;

[0024] Step S2: liquid injection: the support is driven to slide on the reaction disc cover by the driving assembly, the support drives the liquid injection head to move until the liquid injection head moves above the specified liquid injection position of the incubation module, the preheated to-be-injected material enters the liquid injection head from the heating pipeline and is then injected into the reaction cup rotated to the specified liquid injection position;

[0025] Step S3: replacing the to-be-injected material: the reaction cups are sequentially rotated to the designated injection position, and the heated to-be-injected material is injected into each reaction cup through the injection head until the to-be-injected material in the liquid bottle assembly is exhausted, the communication between the liquid bottle assembly and the liquid discharge pipeline is cut off, and the to-be-injected material is replaced;

[0026] Step S4: cleaning the liquid discharge pipeline: the cleaning liquid bottle and the liquid discharge pipeline are communicated, the heating of the heating element is turned off, the cleaning liquid enters the liquid discharge pipeline from the cleaning liquid bottle to clean, forms cleaning waste liquid, and then enters the heating pipeline;

[0027] Step S5: discharging the cleaning waste liquid: the driving assembly drives the bracket to slide on the reaction disc cover, the bracket drives the injection head to move on the reaction disc cover until the injection head moves above the waste liquid tank, the cleaning waste liquid enters the injection head from the heating pipeline and is then injected into the waste liquid tank, the cleaning waste liquid is discharged, and the communication between the cleaning liquid bottle and the liquid discharge pipeline is cut off.

[0028] The present application has the following advantages:

[0029] The immune analysis device provided by the present application comprises an incubation module and an injection module, the incubation module comprises a reaction disc cover, the injection module comprises a bracket, an injection assembly, a heating assembly and a driving assembly, the driving assembly can drive the bracket to slide on the reaction disc cover, the injection assembly is installed on the bracket, the injection assembly comprises an injection head and a liquid bottle assembly, the liquid bottle assembly stores a to-be-injected material, the heating assembly comprises a heating pipeline and a heating element, the heating element can heat the heating pipeline, one end of the heating pipeline can be communicated with the liquid bottle assembly, and the other end is communicated with the injection head. The to-be-injected material is heated by the heating assembly and then injected into the reaction cup through the injection assembly, so that the to-be-injected material does not need to be heated in the reaction cup, the incubation time is shortened, and the incubation effect is ensured.

[0030] The injection method using the injection module provided by the present application further comprises a cleaning assembly, the cleaning assembly comprises a cleaning liquid bottle storing cleaning liquid, the injection assembly further comprises a liquid discharge pipeline, one end of the heating pipeline can be communicated with the liquid bottle assembly through the liquid discharge pipeline, and the cleaning liquid bottle and the liquid bottle assembly are selectively communicated on the liquid discharge pipeline. The injection method comprises the following steps:

[0031] Step S1: preheating the to-be-injected material: the liquid bottle assembly and the liquid discharge pipeline are communicated, the to-be-injected material enters the liquid discharge pipeline from the liquid bottle assembly, the heating assembly is started, and the to-be-injected material enters the heating pipeline from the liquid bottle assembly and is preheated to a specified temperature;

[0032] Step S2: injection: the driving assembly drives the bracket to slide on the reaction disc cover, the bracket drives the injection head to move until the injection head moves above the designated injection position of the incubation module, the preheated to-be-injected material enters the injection head from the heating pipeline and is then injected into the reaction cup rotated to the designated injection position;

[0033] Step S3: replacing the to-be-injected material: the reaction cups are sequentially rotated to the designated injection position, and the heated to-be-injected material is injected into each reaction cup through the injection head until the to-be-injected material in the liquid bottle assembly is exhausted, the communication between the liquid bottle assembly and the drainage pipeline is cut off, and the to-be-injected material is replaced;

[0034] Step S4: cleaning the drainage pipeline: the cleaning liquid bottle and the drainage pipeline are communicated, the heating of the heating element is turned off, the cleaning liquid enters the drainage pipeline from the cleaning liquid bottle to clean, forms cleaning waste liquid, and then enters the heating pipeline;

[0035] Step S5: discharging the cleaning waste liquid: the driving assembly drives the bracket to slide on the reaction disc cover, the bracket drives the injection head to move on the reaction disc cover until the injection head moves above the waste liquid tank, the cleaning waste liquid enters the injection head from the heating pipeline and is then injected into the waste liquid tank, the cleaning waste liquid is discharged, and the communication between the cleaning liquid bottle and the drainage pipeline is cut off.

[0036] The injection method is realized through the injection module, the injection, cleaning and replacement of the to-be-injected material can be realized, the incubation in the immune analysis process is facilitated, and the immune analysis efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0037] Figure 1 is a structural schematic diagram of an immune analysis equipment provided by an embodiment of the present application;

[0038] Figure 2 is a structural schematic diagram of an injection module and a reaction disc cover provided by an embodiment of the present application;

[0039] Figure 3 is a structural schematic diagram of an injection module provided by an embodiment of the present application;

[0040] Figure 4 is a partial structural schematic diagram of an injection module provided by an embodiment of the present application;

[0041] Figure 5 is a structural schematic diagram of a heating assembly provided by an embodiment of the present application;

[0042] Figure 6 is an exploded view of a heating assembly provided by an embodiment of the present application;

[0043] Figure 7 is a structural schematic diagram of a heating pipeline provided by an embodiment of the present application;

[0044] Figure 8 is a top view of an injection module provided by an embodiment of the present application;

[0045] Figure 9 is a structural schematic diagram of a moving module provided by an embodiment of the present application.

[0046] In the drawings:

[0047] 100, liquid injection module; 200, incubation module; 201, reaction disc cover; 202, liquid injection hole; 300, rack;

[0048] 1, support; 2, liquid injection assembly; 3, heating assembly; 4, cleaning assembly; 5, push block; 6, reset assembly; 7, detection assembly; 8, drag chain assembly; 9, slide rail; 10, driving assembly;

[0049] 20, bubble sensor; 21, liquid injection head; 22, fixed block; 23, liquid bottle assembly; 24, liquid injection switching valve; 25, plunger pump; 26, liquid inlet and outlet valve; 27, liquid inlet pipeline; 28, communication pipeline; 29, liquid outlet pipeline; 31, heating pipeline; 32, heating piece; 33, fixed seat; 34, heating cover plate; 35, temperature controller switch; 36, temperature sensor; 41, cleaning liquid bottle; 42, cleaning switching valve; 43, cleaning pipeline; 51, insertion hole; 61, tension spring; 71, travel switch; 72, mounting seat; 81, drag chain; 82, first fixed plate; 83, second fixed plate; 101, pipette; 102, first moving assembly; 103, second moving assembly; 104, third moving assembly;

[0050] 231, first liquid bottle; 232, second liquid bottle; 233, liquid bottle drawer; 271, first liquid inlet pipe; 272, second liquid inlet pipe; 281, first communication pipe; 282, second communication pipe; 311, first port; 312, second port; 331, heating pipe groove; 811, fixed end; 812, moving end. DETAILED DESCRIPTION

[0051] The technical solutions of the present application will be further described below in combination with the drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, but not all.

[0052] In the present application, some orientation words are defined. Without making the opposite statement, the orientation words such as "up", "down", "left", "right", "inner", "outer" are used for the convenience of understanding, and thus do not constitute the limitation to the protection scope of the present application.

[0053] In the present application, unless otherwise explicitly specified and limited, "on" or "under" of a first feature to a second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, "on", "above" and "over" of a first feature to a second feature includes that the first feature is directly above and obliquely above the second feature, or only indicates that the first feature is higher than the second feature in horizontal height. "Under", "below" and "underneath" of a first feature to a second feature includes that the first feature is directly below and obliquely below the second feature, or only indicates that the first feature is lower than the second feature in horizontal height.

[0054] In the description of the present application, unless otherwise explicitly specified and limited, the terms "connected", "connected", "fixed" should be understood broadly, for example, can be fixedly connected, or can be detachably connected, or integrated; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0055] As shown in Figure 1 The present embodiment provides an immunoassay device, which comprises a liquid injection module 100, an incubation module 200 and a rack 300, and the liquid injection module 100 and the incubation module 200 are installed in the rack 300. The incubation module 200 comprises a reaction disc and a reaction disc cover 201, a plurality of reaction cups are placed in the reaction disc, and the reaction disc cover 201 is arranged on the opening of the reaction disc. The reaction disc can rotate relative to the reaction disc cover 201. When the reaction disc rotates, the reaction cups are driven to rotate, so that the liquid injection module 100 injects the to-be-injected substance into each reaction cup.

[0056] As shown in Figure 2 Specifically, the reaction disc cover 201 is provided with a liquid injection hole 202, and the reaction disc drives each reaction cup to rotate to the position below the liquid injection hole 202. The liquid injection module 100 injects the to-be-injected substance into each reaction cup through the liquid injection hole 202.

[0057] As shown in Figures 2-9As shown, the liquid injection module 100 comprises a bracket 1, a liquid injection assembly 2, a heating assembly 3, a cleaning assembly 4 and a driving assembly 10. The bracket 1 is slidably arranged on the reaction disc cover 201. The liquid injection assembly 2 is mounted on the bracket 1. The liquid injection assembly 2 comprises a liquid injection head 21 and a liquid bottle assembly 23. The liquid bottle assembly 23 stores the to-be-injected substance. The heating assembly 3 comprises a heating pipeline 31 and a heating element 32. The heating element 32 can heat the heating pipeline 31. One end of the heating pipeline 31 can be in communication with the liquid bottle assembly 23, and the other end is in communication with the liquid injection head 21. The driving assembly 10 can drive the bracket 1 to slide. The to-be-injected substance is heated by the heating assembly 3, and then injected into each reaction cup through the liquid injection assembly 2. The to-be-injected substance does not need to be heated in the reaction cup, which shortens the incubation time and ensures the incubation effect.

[0058] Specifically, the liquid injection head 21 can extend into the liquid injection hole 202 and inject the heated to-be-injected substance into the reaction cup located below the liquid injection hole 202.

[0059] In this embodiment, the to-be-injected substance refers to a general liquid. Of course, the to-be-injected substance can also be other reagents, which are not limited and can be set according to actual conditions.

[0060] In this embodiment, the liquid injection head 21 can be a hose. When the to-be-injected substance drips from the liquid injection head 21, if pipe mouth condensate appears, a small section with pipe mouth condensate can be cut off in a short-cut manner to prevent blockage and ensure normal injection.

[0061] Further, the driving assembly 10 is mounted on the rack 300 and can move relative to the reaction disc cover 201 to facilitate sliding of the bracket 1.

[0062] Further, the liquid bottle assembly 23 comprises a first liquid bottle 231, a second liquid bottle 232 and a liquid bottle drawer 233. The liquid bottle drawer 233 is mounted in the rack 300, and the first liquid bottle 231 and the second liquid bottle 232 are arranged in the liquid bottle drawer 233.

[0063] As shown in Figure 2 and Figure 3 , the liquid injection module 100 further comprises a push block 5 mounted on the bracket 1. The push block 5 is provided with a insertion hole 51. The driving assembly 10 can be connected with the insertion hole 51. When the driving assembly 10 is connected with the insertion hole 51, the driving assembly 10 can drive the push block 5 and the bracket 1 to move, thereby driving the liquid injection head 21 to move on the reaction disc cover 201, so as to move the liquid injection head 21 above the liquid injection hole 202 to facilitate injection.

[0064] Further, the driving assembly 10 comprises a pipette 101 (as shown in Figure 9 . The pipette 101 can be movably arranged relative to the reaction disc cover 201. The pipette 101 can be inserted into the insertion hole 51.

[0065] Further, the liquid injection module 100 further comprises a drag chain assembly 8 and a slide rail 9, the drag chain assembly 8 comprises a drag chain 81, the slide rail 9 is fixedly installed on the reaction disc cover 201, a fixed end 811 of the drag chain 81 is fixedly installed on the reaction disc cover 201, a moving end 812 of the drag chain 81 is fixedly installed on the bracket 1, and the bracket 1 is slidably arranged on the slide rail 9. The drag chain 81 is provided with a wire or a pipeline, which can prevent the wire or the pipeline from being damaged.

[0066] Further, the drag chain assembly 8 further comprises a first fixing plate 82, the first fixing plate 82 is fixedly installed on the reaction disc cover 201, and the fixed end 811 of the drag chain 81 is fixedly installed on the first fixing plate 82.

[0067] Further, the drag chain assembly 8 further comprises a second fixing plate 83, the second fixing plate 83 is fixedly installed on the bracket 1, and the moving end 812 of the drag chain 81 is fixedly installed on the second fixing plate 83.

[0068] Further, the liquid injection assembly 2 further comprises a fixing block 22, the fixing block 22 is installed on the bracket 1 and located above the liquid injection hole 202, and the liquid injection head 21 is guided by the fixing block 22.

[0069] Specifically, the fixing block 22 is provided with a guide groove, the liquid injection head 21 can pass through the guide groove and be inserted into the liquid injection hole 202 to inject the heated to-be-injected substance into the reaction cup.

[0070] As shown in FIG. 1, Figure 4 The liquid injection module 100 further comprises a cleaning assembly 4, the cleaning assembly 4 comprises a cleaning liquid bottle 41 storing cleaning liquid, the liquid injection assembly 2 further comprises a liquid discharge pipeline 29, the cleaning liquid bottle 41 and the liquid bottle assembly 23 are selectively communicated with the liquid discharge pipeline 29, and the liquid discharge pipeline 29 is communicated with one end of the heating pipeline 31.

[0071] Further, the cleaning assembly 4 further comprises a cleaning switch valve 42, a first inlet of the cleaning switch valve 42 is communicated with the liquid bottle assembly 23, a second inlet of the cleaning switch valve 42 is communicated with the cleaning liquid bottle 41, an outlet of the cleaning switch valve 42 is communicated with the liquid discharge pipeline 29, and the first inlet and the second inlet of the cleaning switch valve 42 are selectively communicated with the outlet of the cleaning switch valve 42.

[0072] Further, the cleaning assembly 4 further comprises a cleaning pipeline 43, the cleaning liquid bottle 41 is communicated with the second inlet of the cleaning switch valve 42 through the cleaning pipeline 43.

[0073] Further, the liquid injection assembly 2 further comprises a communication pipeline 28, the communication pipeline 28 is used for connecting the liquid bottle assembly 23, the cleaning switch valve 42 and the liquid discharge pipeline 29.

[0074] Specifically, the communication pipeline 28 comprises a first communication pipeline 281, the liquid bottle assembly 23 is communicated with the first inlet of the cleaning switch valve 42 through the first communication pipeline 281.

[0075] Specifically, the communication pipeline 28 further comprises a second communication pipeline 282, the outlet of the cleaning switch valve 42 is communicated with the liquid discharge pipeline 29 through the second communication pipeline 282.

[0076] Further, the liquid injection assembly 2 further comprises a liquid injection switch valve 24, the liquid bottle assembly 23 comprises a first liquid bottle 231 and a second liquid bottle 232, the first liquid bottle 231 and the second liquid bottle 232 both store the liquid to be injected, the first liquid bottle 231 is communicated with the first inlet of the liquid injection switch valve 24, the second liquid bottle 232 is communicated with the second inlet of the liquid injection switch valve 24, the outlet of the liquid injection switch valve 24 is communicated with the first inlet of the cleaning switch valve 42, the first inlet and the second inlet of the liquid injection switch valve 24 are selectively communicated with the outlet of the liquid injection switch valve 24.

[0077] Further, the liquid injection assembly 2 further comprises a liquid inlet pipeline 27, the liquid bottle assembly 23 is communicated with the liquid injection switch valve 24 through the liquid inlet pipeline 27.

[0078] Specifically, the liquid inlet pipeline 27 comprises a first liquid inlet pipeline 271, the first liquid bottle 231 is communicated with the first inlet of the liquid injection switch valve 24 through the first liquid inlet pipeline 271.

[0079] Specifically, the liquid inlet pipeline 27 comprises a second liquid inlet pipeline 272, the second liquid bottle 232 is communicated with the second inlet of the liquid injection switch valve 24 through the second liquid inlet pipeline 272.

[0080] Further, the liquid injection assembly 2 further comprises a bubble sensor 20, the bubble sensor 20 is arranged on the second communication pipeline 282.

[0081] Further, the liquid injection assembly 2 further comprises a plunger pump 25 and a liquid inlet and outlet valve 26, the plunger pump 25 is arranged between the second communication pipeline 282 and the liquid discharge pipeline 29 through the liquid inlet and outlet valve 26.

[0082] Specifically, the first through port of the liquid inlet and outlet valve 26 is communicated with the second communication pipeline 282, the second through port of the liquid inlet and outlet valve 26 is communicated with the liquid discharge pipeline 29, the third through port of the liquid inlet and outlet valve 26 is communicated with the plunger pump 25, the first through port and the second through port of the liquid inlet and outlet valve 26 are selectively communicated with the third through port of the liquid inlet and outlet valve 26. The plunger pump 25 sucks the liquid (which can be the liquid to be injected or the cleaning waste liquid) in the second communication pipeline 282 into the plunger pump 25, and then pumps the liquid sucked into the plunger pump 25 into the liquid discharge pipeline 29.

[0083] As Figures 5-7As shown, the heating assembly 3 further comprises a fixing base 33, the fixing base 33 is provided with a heating pipe groove 331 in a serpentine distribution, the heating pipe 31 is installed in the heating pipe groove 331, and the heating element 32 is attached to the heating pipe 31. The serpentine distribution can increase the contact area of the heating pipe 31 and the heating element 32, and improve the heat conduction efficiency of the heating element 32 and the heating pipe 31.

[0084] Further, since the heat conduction efficiency of the heating element 32 and the heating pipe 31 is good, a heating element 32 with smaller power can be selected.

[0085] Further, the heating assembly 3 further comprises a heating cover plate 34, and the heating element 32 is installed between the heating cover plate 34 and the fixing base 33.

[0086] Further, the heating assembly 3 further comprises a temperature controller switch 35, and the temperature controller switch 35 is installed on the fixing base 33.

[0087] Further, the heating assembly 3 further comprises a temperature sensor 36, the temperature sensor 36 can detect the temperature of the to-be-injected material in the heating pipe 31, and the temperature sensor 36 is installed on the fixing base 33.

[0088] As shown in the drawings, Figure 7 The heating pipe 31 comprises a first port 311 and a second port 312, the first port 311 and the second port 312 both extend out of the heating pipe groove 331, and one of the first port 311 and the second port 312 is in communication with the liquid discharge pipe 29, and the other is in communication with the liquid injection head 21.

[0089] As shown in the drawings, Figure 8 The liquid injection module 100 further comprises a reset assembly 6, the reset assembly 6 comprises a tension spring 61, one end of the tension spring 61 is fixed relative to the reaction disc cover 201, and the other end is fixed relative to the support 1. When there is no to-be-injected material, the support 1 moves towards the liquid injection hole 202 under the action of the tension spring 61, so that the liquid injection hole 202 is blocked by the support 1, which can prevent the reaction cup in the reaction disc from being contaminated; when it is necessary to inject the to-be-injected material, the liquid injection gun 101 moves away from the liquid injection hole 202, so that the fixed block 22 is opposite to the liquid injection hole 202, and then the liquid injection head 21 is inserted into the liquid injection hole 202 to inject the to-be-injected material into the reaction cup.

[0090] Further, the liquid injection module 100 further comprises a detection assembly 7, the detection assembly 7 is used for detecting whether the support 1 reaches a preset position or not, so as to inject the liquid.

[0091] The detection assembly 7 comprises a travel switch 71, and the travel switch 71 is installed on the support 1.

[0092] Furthermore, the detection assembly 7 also includes a mounting seat 72 , which is mounted on the bracket 1 , and the travel switch 71 is mounted on the mounting seat 72 .

[0093] like Figure 9 As shown, the drive assembly 10 further includes a first movable assembly 102, a second movable assembly 103, and a third movable assembly 104. The third movable assembly 104 is mounted on the frame 300. The second movable assembly 103 can be slidably disposed on the third movable assembly 104 along the sliding direction of the bracket 1. The first movable assembly 102 can be slidably disposed on the second movable assembly 103 in a direction perpendicular to the sliding direction of the bracket 1. The pipette 101 can be slidably disposed on the first movable assembly 102 in a vertical direction. The pipette 101 can be inserted into the insertion hole 51 and drive the push block 5 and the bracket 1 to move so as to inject liquid into the reaction cups in the reaction tray.

[0094] In this embodiment, the specific structures of the first moving component 102, the second moving component 103 and the third moving component 104 are not limited. Common linear mechanisms (such as screw-nut mechanisms, screw-slider mechanisms, etc.) can be selected as long as the pipette gun 101 can be moved in three-dimensional space.

[0095] This embodiment also provides a liquid injection method, which is implemented by the liquid injection module 100 and includes the following steps:

[0096] Step S1: Preheating the material to be filled: connecting the liquid bottle assembly 23 and the liquid discharge pipe 29, allowing the material to be filled to flow from the liquid bottle assembly 23 into the liquid discharge pipe 29, starting the heating assembly 3, and allowing the material to flow from the liquid bottle assembly 23 through the liquid discharge pipe 29 into the heating pipe 31 to be preheated to a specified temperature;

[0097] Step S2: Liquid Injection: The drive assembly 10 drives the bracket 1 to slide on the reaction tray cover 201. The bracket 1 drives the liquid injection head 21 to move until the liquid injection head 21 moves above the designated liquid injection position of the incubation module 200. The preheated liquid to be injected enters the liquid injection head 21 through the heating pipe 31 and is then injected into the reaction cup rotated to the designated liquid injection position.

[0098] Step S3: Replacing the substance to be filled: The reaction cups are rotated to the designated injection positions in sequence, and the heated substance to be filled is injected into each reaction cup through the injection head 21 until the substance to be filled in the liquid bottle assembly 23 is exhausted. The connection between the liquid bottle assembly 23 and the drainage pipe 29 is then cut off, and the substance to be filled is replaced.

[0099] Step S4: Cleaning the drain pipe 29: Connect the cleaning liquid bottle 41 and the drain pipe 29, turn off the heating of the heating element 32, and the cleaning liquid flows from the cleaning liquid bottle 41 into the drain pipe 29 for cleaning, forming a cleaning waste liquid, and then enters the heating pipe 31;

[0100] Step S5: discharging the cleaning waste liquid: the driving assembly 10 drives the bracket 1 to slide on the reaction disc cover 201, and the bracket 1 drives the liquid injection head 21 to move on the reaction disc cover 201 until the liquid injection head 21 moves above the waste liquid tank, the cleaning waste liquid enters the liquid injection head 21 from the heating pipeline 31 and is then injected into the waste liquid tank, the discharging of the cleaning waste liquid is completed, and the communication between the cleaning liquid bottle 41 and the liquid discharging pipeline 29 is cut off.

[0101] The liquid injection method is realized by the liquid injection module 100, and the liquid injection, cleaning and replacement of the to-be-injected substance can be realized, the incubation in the immune analysis process is facilitated, and the immune analysis efficiency is improved.

[0102] In step S1, the specific steps of the communication between the liquid bottle assembly 23 and the liquid discharging pipeline 29 are as follows:

[0103] One of the first liquid bottle 231 and the second liquid bottle 232 is selected to be communicated with the first communication pipeline 281, the first inlet and the outlet of the cleaning switch valve 42 are communicated, the plunger pump 25 sucks the to-be-injected substance in the first liquid bottle 231 or the second liquid bottle 232 into the plunger pump 25, and then pumps the to-be-injected substance into the liquid discharging pipeline 29. If the first liquid bottle 231 is selected, the first inlet and the outlet of the liquid injection switch valve 24 are communicated; if the second liquid bottle 232 is selected, the second inlet and the outlet of the liquid injection switch valve 24 are communicated.

[0104] In step S2, the above of the specified liquid injection position of the incubation module 200 refers to the above of the liquid injection hole 202, and the reaction cup at the specified liquid injection position refers to the reaction cup located in the reaction disc and below the liquid injection hole 202.

[0105] In step S3, the exhaustion of the to-be-injected substance in the liquid bottle assembly 23 refers to the exhaustion of the to-be-injected substance in the liquid bottle communicated with the liquid discharging pipeline 29.

[0106] The replacement of the to-be-injected substance refers to that the liquid injection switch valve 24 can switch the communication between the first liquid bottle 231 and the second liquid bottle 232 and the first communication pipeline 281, so as to control the communication between the first liquid bottle 231 and the second liquid bottle 232 and the liquid discharging pipeline 29, without stopping the machine to replace the liquid bottle separately, and the incubation efficiency is improved.

[0107] The cut-off of the communication between the liquid bottle assembly 23 and the liquid discharging pipeline 29 refers to that the second inlet and the outlet of the cleaning switch valve 42 are communicated, and the cleaning step is prepared.

[0108] After the replacement of the liquid bottle, in order to ensure the accuracy of the immune analysis result, the to-be-injected substance in the replaced liquid bottle needs to be sucked into the second communication pipeline 282 and the liquid discharging pipeline 29 after the cleaning step in steps S4 and S5 is performed to clean the second communication pipeline 282 and the liquid discharging pipeline 29.

[0109] In step S4, the following steps are included:

[0110] The second inlet and the outlet of the cleaning switch valve 42 are communicated, the plunger pump 25 pumps the cleaning liquid in the cleaning liquid bottle 41 into the liquid discharge pipeline 29 through the second communication pipe 282, forming cleaning waste liquid, and then entering the heating pipeline 31.

[0111] Although the present application has been described in detail with general description, specific embodiments and experiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application claimed.

Claims

1. A liquid injection module characterized by comprising: The injection module (100) comprises: a support (1) configured to be slidably arranged on a reaction disc cover (201); an injection assembly (2) mounted on the support (1), the injection assembly (2) comprising an injection head (21) and a liquid bottle assembly (23) in which a to-be-injected substance is stored; a heating assembly (3) comprising a heating pipeline (31) and a heating element (32), the heating element (32) being capable of heating the heating pipeline (31), one end of the heating pipeline (31) being capable of communicating with the liquid bottle assembly (23), and the other end communicating with the injection head (21); a driving assembly (10) capable of driving the support (1) to slide.

2. The liquid injection module of claim 1, wherein, The injection module (100) further comprises a push block (5) mounted on the support (1), the push block (5) being provided with a insertion hole (51), and the driving assembly (10) is capable of being connected with the insertion hole (51).

3. The liquid injection module of claim 2, wherein, The driving assembly (10) comprises a pipette gun (101) movably arranged relative to the reaction disc cover (201), and the pipette gun (101) is capable of being inserted into the insertion hole (51).

4. The liquid injection module of claim 1, wherein, The heating assembly (3) further comprises a fixing seat (33) provided with a heating pipeline groove (331) in a serpentine distribution, the heating pipeline (31) is mounted in the heating pipeline groove (331), and the heating element (32) is attached to the heating pipeline (31).

5. The liquid injection module of claim 4, wherein, The heating assembly (3) further comprises a heating cover plate (34), and the heating element (32) is mounted between the heating cover plate (34) and the fixing seat (33).

6. The liquid injection module of claim 1, wherein, The injection module (100) further comprises a reset assembly (6) comprising a tension spring (61), one end of the tension spring (61) being fixed relative to the reaction disc cover (201), and the other end being fixed relative to the support (1).

7. The liquid injection module of claim 1, wherein, The injection module (100) further comprises a cleaning assembly (4) comprising a cleaning liquid bottle (41) storing cleaning liquid, the injection assembly (2) further comprises a drainage pipeline (29), and the cleaning liquid bottle (41) and the liquid bottle assembly (23) are selectively communicated with the drainage pipeline (29), and the drainage pipeline (29) communicates with one end of the heating pipeline (31).

8. The liquid injection module of claim 1, wherein, The injection module (100) further comprises a drag chain assembly (8) and a sliding rail (9), the drag chain assembly (8) comprises a drag chain (81), the sliding rail (9) is fixedly mounted on the reaction disc cover (201), a fixed end (811) of the drag chain (81) is fixedly mounted on the reaction disc cover (201), a moving end (812) is fixedly mounted on the support (1), and the support (1) is slidably arranged on the sliding rail (9).

9. An immunoassay device characterized by, The injection module (100) comprises: an incubation module (200) comprising a reaction disc cover (201) ； The injection module (100) according to any one of claims 1-8.

10. A liquid injection method using the liquid injection module according to any one of claims 1 to 8, characterized by, The liquid injection module further comprises a cleaning assembly (4), the cleaning assembly (4) comprises a cleaning liquid bottle (41) storing cleaning liquid, the liquid injection assembly (2) further comprises a liquid discharge pipeline (29), one end of the heating pipeline (31) can communicate with the liquid bottle assembly (23) through the liquid discharge pipeline (29), the cleaning liquid bottle (41) and the liquid bottle assembly (23) can selectively communicate with the liquid discharge pipeline (29), and the liquid injection method comprises the following steps: Step S1: preheating the to-be-injected material: the liquid bottle assembly (23) and the liquid discharge pipeline (29) are communicated, the to-be-injected material enters the liquid discharge pipeline (29) from the liquid bottle assembly (23), the heating assembly (3) is started, and the to-be-injected material enters the heating pipeline (31) from the liquid bottle assembly (23) and is preheated to a specified temperature; Step S2: liquid injection: the support (1) is driven to slide on the reaction disc cover (201) by the driving assembly (10), the liquid injection head (21) is driven to move by the support (1), until the liquid injection head (21) moves above the specified liquid injection position of the incubation module (200), the preheated to-be-injected material enters the liquid injection head (21) from the heating pipeline (31) and is then injected into the reaction cup rotated to the specified liquid injection position; Step S3: replacing the to-be-injected material: the reaction cup is rotated to the specified liquid injection position in sequence, and the heated to-be-injected material is injected into each reaction cup through the liquid injection head (21), until the to-be-injected material in the liquid bottle assembly (23) is exhausted, the communication between the liquid bottle assembly (23) and the liquid discharge pipeline (29) is cut off, and the to-be-injected material is replaced; Step S4: cleaning the liquid discharge pipeline (29): the cleaning liquid bottle (41) and the liquid discharge pipeline (29) are communicated, the heating of the heating element (32) is turned off, the cleaning liquid enters the liquid discharge pipeline (29) from the cleaning liquid bottle (41) to clean, forms cleaning waste liquid, and then enters the heating pipeline (31); Step S5: discharging the cleaning waste liquid: the support (1) is driven to slide on the reaction disc cover (201) by the driving assembly (10), the liquid injection head (21) is driven to move on the reaction disc cover (201) by the support (1), until the liquid injection head (21) moves above the waste liquid tank, the cleaning waste liquid enters the liquid injection head (21) from the heating pipeline (31) and is then injected into the waste liquid tank, the cleaning waste liquid is discharged, and the communication between the cleaning liquid bottle (41) and the liquid discharge pipeline (29) is cut off.

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